Template-Type: ReDIF-Article 1.0 Author-Name: K-H Do Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: M-G Kim Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: D-H Ryu Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: H-J Ahn Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: S-B Kim Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: Y-K Go Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: SY Kim Author-Workplace-Name: Division of Zoonotic and Vector-Borne Diseases Research, Center for Infectious Diseases Research, Korea National Institute of Health, Cheongju, Republic of Korea Author-Name: S Kim Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: S-H Lee Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: D-K Kim Author-Workplace-Name: Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, Republic of Korea Author-Name: Y-E Cho Author-Workplace-Name: Department of Food and Nutrition, Kyungkook National University, Andong, Republic of Korea Author-Name: J Kim Author-Workplace-Name: School of Integrative Engineering, Chung-Ang University, Seoul, Republic of Korea Author-Name: YK Park Author-Workplace-Name: Department of Biomedical Comparative Science, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA Author-Name: K Youn Author-Workplace-Name: KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Dongdaemun-gu, Seoul, Republic of Korea Author-Name: H Oh Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: K-W Seo Author-Workplace-Name: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Title: Additive effects of ceftiofur-neomycin combination against multidrug-resistant pathogenic Escherichia coli in a murine infection model Abstract: This study aimed to evaluate the therapeutic efficacy of a ceftiofur-neomycin combination against a pathogenic multidrug-resistant Escherichia coli strain, KECS-0513, isolated from pigs, using a combination of genomic, in vitro, and in vivo approaches. The minimum inhibitory concentration, minimum bactericidal concentration, and checkerboard assays were performed. Time-kill assays were used to assess bactericidal activity over time, and a murine intraperitoneal infection model was used to evaluate survival outcomes following treatment with monotherapies or combination regimens. Whole genome sequencing indicated that KECS-0513 harboured multiple resistance genes, including blaTEM-1B, sul3, aadA12, aad(3)-IVa, aph(3')-Ia, and aph(4)-Ia. The resistance genes were localised within a mobile, element-rich plasmid. In vitro checkerboard assays revealed an additive interaction between ceftiofur and neomycin (fractional inhibitory concentration index = 1.0), and time-kill experiments demonstrated enhanced and sustained bacterial clearance with combination therapy. In vivo infection studies in mice demonstrated that the dual treatment resulted in a substantially higher survival rate (66.7%) compared to treatment with either agent alone (33.3% for each). These results support the practical application of ceftiofur-neomycin combination therapy for controlling swine-associated multidrug-resistant E. coli while minimising the risk of resistance emergence. Keywords: aminoglycosides, antimicrobial resistance, beta-lactams, combination therapy, whole-genome sequencing Journal: Veterinární medicína Pages: 18-26 Volume: 71 Issue: 1 Year: 2026 DOI: 10.17221/38/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/38/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202601-0001.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:1:id:38-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y Jeong Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea Author-Name: D Kwon Author-Workplace-Name: Bundang Leaders Animal Medical Centre, Seongnam, Republic of Korea Author-Name: K Park Author-Workplace-Name: Bundang Leaders Animal Medical Centre, Seongnam, Republic of Korea Author-Name: K Lee Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea Author-Name: H Yoon Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea Author-Workplace-Name: Biosafety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea Title: Presumed atypical cor triatriatum dexter in a geriatric dog: A case report Abstract: Membranous structures within the right atrium (RA), such as the Chiari network, eustachian valve, Thebesian valve, and cor triatriatum dexter (CTD), can present with overlapping imaging features and complicated diagnoses. A 17-year-old male Maltese presented with a cardiac murmur, cough, anorexia, and exercise intolerance. Echocardiography revealed a mobile membrane in the RA, dividing it into two chambers. Computed tomography (CT) confirmed this finding, demonstrating differential attenuation of contrast between the chambers. The vascular connections and anatomical relationships of the structure differed from those of previously reported CTD types. Although other sinus venosus valve remnants were considered, the findings supported a provisional diagnosis of atypical CTD. This case highlights the utility of multimodal imaging for the characterisation of right atrial membranous structures. In human medicine, a detailed anatomical comparison of sinus venosus valve remnants aids in the differential diagnosis. Applying a similar approach in veterinary medicine, especially in geriatric patients, may improve diagnostic accuracy. Comprehensive imaging evaluations, including echocardiography and CT, are recommended to assess RA membranes that may be misinterpreted as variants of the CTD or other embryological remnants. Keywords: computed tomography, congenital heart disease, echocardiography, right sinus venosus valve remnants Journal: Veterinární medicína Pages: 27-34 Volume: 71 Issue: 1 Year: 2026 DOI: 10.17221/50/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/50/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202601-0002.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:1:id:50-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F Sevilla Author-Workplace-Name: Doctorado en Ciencias Naturales para el Desarrollo (DOCINADE), Instituto Tecnológico de Costa Rica, Universidad Nacional, Universidad Estatal a Distancia, Alajuela, Costa Rica Author-Workplace-Name: Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, Alajuela, Costa Rica Author-Name: I Araya-Zuniga Author-Workplace-Name: Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, Alajuela, Costa Rica Author-Name: JM Solis Author-Workplace-Name: Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, Alajuela, Costa Rica Author-Name: C Corcini Author-Workplace-Name: Veterinary School, Pelotas Federal University, Rio Grande do Sul, Brazil Author-Name: P Cervantes-Acosta Author-Workplace-Name: School of Veterinary Medicine and Animal Husbandry, Veracruz University, Veracruz, México Author-Name: A Hernandez-Beltran Author-Workplace-Name: School of Veterinary Medicine and Animal Husbandry, Veracruz University, Veracruz, México Author-Name: R Molina-Montero Author-Workplace-Name: Agricultural Production Program, School of Agronomy, Costa Rica Institute of Technology, Alajuela, Costa Rica Author-Name: D Pichardo-Matamoros Author-Workplace-Name: Animal Husbandry Engineering, National Agrarian University, Chontales, Nicaragua Author-Name: A Valverde Author-Workplace-Name: Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, Alajuela, Costa Rica Title: Dairy bull sperm subpopulation behaviour in frozen-thawed semen across breed, temperature, and thawing time Abstract: Optimising dairy cattle reproduction with assisted reproductive technologies, such as artificial insemination, requires standardised semen handling and analysis. This study evaluated the kinematic structure of the spermatozoan subpopulation in frozen-thawed Holstein and Jersey bull semen doses under different thawing protocols. We used frozen semen doses taken from four bulls of each breed. Nine semen doses were collected from each animal, for a total of 72 cryopreserved doses. Straw thawing was performed at three temperatures (35 °C, 37 °C, and 40 °C) and three times (30 s, 40 s, and 45 s). Sperm kinematic patterns were evaluated using a CASA-mot system (ISAS®v1). Sperm kinematic variables for each breed, temperature, and thawing time identified four subpopulations. The analysis revealed an effect of breed and semen thawing protocols (P < 0.05) on sperm subpopulation distribution, sperm movement, and swimming patterns. Subpopulation analyses based on semen assessment are needed to further interpret the relevance and effect on fertility. Keywords: cryopreservation, dairy cattle, reproduction, spermatozoa, sperm kinematics Journal: Veterinární medicína Pages: 1-9 Volume: 71 Issue: 1 Year: 2026 DOI: 10.17221/56/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/56/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202601-0003.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:1:id:56-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Matiasovic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M Zouharova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: P Strakova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L Kavanova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: D Karasova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J Gebauer Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A Csorgo Author-Workplace-Name: ARVET, Gabcikovo, Slovak Republic Author-Name: I Rychlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: The safety of fermented milk as a feedback method to reduce diarrhoea in newborn piglets Abstract: Diarrhoea in newborn piglets represents a significant challenge to pig production. Controlled oral exposure, also known as "feedback", whereby sows are exposed at least two weeks before farrowing to pathogens that cause health problems in piglets, is a traditional method of diarrhoea prevention. One type of feedback involves fermenting cow's milk with faeces from piglets suffering from diarrhoea and administering it to sows before farrowing. The bacterial composition of the faecal inoculum and fermented milk was compared in this study, and the safety of administering the fermented milk to pregnant sows was evaluated. Using microbiota characterisation by 16S rRNA gene sequencing, the genera Acetobacter, Lactobacillus and Lactococcus formed the core microbiota of the fermented milk. However, Clostridium perfringens accounted for up to 33% of the total microbiota in some fermented milk samples. Interestingly, the drop in pH during the later stages of fermentation inactivated C. perfringens and the samples were thus enriched for inactivated C. perfringens antigen. Our findings contribute to a better understanding of the mode of action of fermented milk when used as a form of feedback. Keywords: Clostridium perfringens, controlled oral exposure, sow Journal: Veterinární medicína Pages: 10-17 Volume: 71 Issue: 1 Year: 2026 DOI: 10.17221/83/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/83/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202601-0004.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:1:id:83-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Editorial Department Title: List of volume 70 reviewers Journal: Veterinární medicína Pages: I-II Volume: 71 Issue: 1 Year: 2026 File-URL: http://vetmed.agriculturejournals.cz/artkey/vet-202601-0005_list-of-volume-70-reviewers.php File-Format: text/html Handle: RePEc:caa:jnlvet:v:71:y:2026:i:1:id:vet-202601-0005