Template-Type: ReDIF-Article 1.0 Author-Name: S Sassaroli Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: F Dini Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: V Sisti Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: V Riccio Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: S Meggiolaro Author-Workplace-Name: Veterinary freelance, Abano Terme, Italy Author-Name: L Bellodi Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: A Palumbo Piccionello Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, Italy Title: Pulsed electromagnetic fields vs NSAID therapy in canine osteoarthritis: A randomised comparative pilot study Abstract: Non-steroidal anti-inflammatory drugs (NSAIDs) are a cornerstone in the management of canine osteoarthritis (OA), despite concerns regarding their long-term safety. Among non-pharmacological alternatives, pulsed electromagnetic field (PEMF) therapy has gained attention for its potential analgesic and anti-inflammatory effects, although veterinary-specific evidence remains limited. This randomised, controlled pilot study compared the clinical efficacy of PEMF therapy versus NSAID treatment (Mavacoxib) in 16 dogs with clinically and radiographically confirmed OA. Dogs were randomly assigned to receive either a 12-session PEMF protocol over 45 days or a standard Mavacoxib regimen. Clinical evaluations included pain scores (VAS), pain on palpation, lameness severity (NRS), gait analysis (GLS and TPI), muscle circumference, and radiographic progression. Assessments were performed at baseline (T0), 30 days (T1), and 60 days (T2). PEMF therapy showed earlier improvements in pain, lameness, and muscle mass, and a favourable trend in joint mobility. No significant differences were detected in gait or radiographic parameters between groups. These preliminary findings suggest that PEMF is non-inferior to NSAIDs and highlight its potential role in multimodal OA management. Limitations include a small sample size and a short follow-up. Further studies are needed to confirm these preliminary results in larger cohorts. Keywords: canine rehabilitation, degenerative joint disease, joint mobility, magnetotherapy, pain assessment Journal: Veterinární medicína Pages: 106-116 Volume: 71 Issue: 3 Year: 2026 DOI: 10.17221/54/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/54/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202603-0001.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:3:id:54-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: AA Zaki Author-Workplace-Name: Department of Medical Biosciences, College of Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia Author-Workplace-Name: Department of Physiology, College of Veterinary Medicine, Cairo University, Cairo, Egypt Author-Name: SM Albarrak Author-Workplace-Name: Department of Pathology and Laboratory Diagnosis, College of Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia Title: Secondary antibody therapy outperforms corticosteroids in an ameliorating lipopolysaccharide-induced rat model of premature ovarian failure Abstract: Premature ovarian failure (POF) is a significant cause of infertility and is often linked to autoimmune aetiologies. Lipopolysaccharide (LPS)-induced inflammation is a well-established model of autoimmune POF in rodents. Immunomodulatory treatments involving corticosteroids, frankincense, and targeted secondary antibodies have been hypothesised to mitigate the autoimmune response, reduce anti-ovarian antibody (AOA) levels, and restore ovarian function in an LPS-induced POF rat model. A POF model was established in female albino rats via the intraperitoneal injection of LPS. The rats were then divided into groups that received no treatment (LPS control), dexamethasone (DEX-treated LPS-treated rats), methylprednisolone (MP-treated LPS-treated rats), frankincense (Frankincense-treated LPS-treated rats), or secondary anti-ovarian antibodies (secondary Ab-treated LPS-treated rats) for 3 to 4 weeks. The serum levels of AOA, 17β-oestradiol, follicle-stimulating hormone (FSH), and luteinising hormone (LH) were assayed via commercial enzyme-linked immunosorbent assay (ELISA) kits. Ovarian tissues were examined histopathologically to assess structural damage and recovery. LPS induction successfully created a POF phenotype, as evidenced by significantly elevated AOA levels (P < 0.001), reduced 17β-oestradiol (P < 0.001), elevated FSH/LH (P < 0.001 and P < 0.05, respectively), and severe histopathological damage, including follicular atresia. All the treatments restored 17β-oestradiol levels. Secondary antibody therapy was most effective, normalising all hormonal parameters, significantly reducing AOA levels, and demonstrating complete histological recovery with healthy follicles and corpora lutea. MP potently suppressed AOA but paradoxically elevated FSH, without improving ovarian histology. DEX and frankincense showed intermediate efficacy, improving some hormonal and serological markers but failing to achieve full histological restoration. These findings demonstrate that targeted immunotherapy using secondary antibodies is superior to broad immunosuppression or anti-inflammatory treatment for restoring ovarian function in patients with autoimmune POF. While corticosteroids effectively reduce AOA titres, they may not reverse ovarian damage and can disrupt the hormonal balance. This underscores the need for precise, biomarker-guided therapies over nonspecific immunosuppression in patients with autoimmune ovarian insufficiency. Keywords: antibodies, autoimmunity, ELISA, frankincense, glucocorticoids Journal: Veterinární medicína Pages: 117-128 Volume: 71 Issue: 3 Year: 2026 DOI: 10.17221/66/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/66/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202603-0002.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:3:id:66-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K Matiaskova Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Reichelova Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Author-Name: E Jeklova Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Zouharova Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: S Kobzova Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: K Nedbalcova Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: J Matiasovic Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Faldyna Author-Workplace-Name: Department of Infectious diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Title: Establishment of an experimental pig model for the induction of a Staphylococcus hyicus skin infection Abstract: Staphylococcus hyicus is one of the causative agents of exudative epidermitis in pigs. The aim of this study was to establish a porcine challenge model of a skin infection caused by S. hyicus to assess the effectiveness of a medicinal product intended for local application. Based on the results of the presence of toxin encoding genes and antimicrobial resistance (detected resistance to clindamycin, penicillin, ampicillin, erythromycin and tetracycline), three field strains were selected for the trial. At D0, six surface defects were created on the back of six piglets. The defects were inoculated with bacteria at two different concentrations: 1 × 108 or 1 × 109 CFU/ml. Every day throughout the experiment, the pigs were monitored, and their rectal temperatures were measured. On D4, D9, and D14, a visual evaluation and indirect bacteriological imprints of the defects were performed. Crusts were present from D9, and a lower bacterial concentration led to reduced secretion and crust formation. Based on the results of bacterial cultivation of the indirect imprints, however, S. hyicus was present in greater amounts in the wound defects infected with the lower bacterial concentration. As there were differences in the obtained results among the strains used, it can be concluded that the strain marked as CAPM 6689 seems to be the most applicable and the lower concentration was enough for the infection development. Keywords: bacteria, exudative epidermitis, Staphylococcaceae, weaning, wounds Journal: Veterinární medicína Pages: 83-94 Volume: 71 Issue: 3 Year: 2026 DOI: 10.17221/68/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/68/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202603-0003.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:3:id:68-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V Karaffova Author-Workplace-Name: Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Author-Name: D Mudronova Author-Workplace-Name: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Author-Name: M Levkut Author-Workplace-Name: Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Author-Name: L Rajcakova Author-Workplace-Name: Research Institute for Animal Production, National Agricultural and Food Centre, Lužianky, Slovak Republic Author-Name: Erik Hudec Author-Workplace-Name: Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Author-Name: R Zitnan Author-Workplace-Name: Research Institute for Animal Production, National Agricultural and Food Centre, Lužianky, Slovak Republic Author-Name: P Patras Author-Workplace-Name: Research Institute for Animal Production, National Agricultural and Food Centre, Lužianky, Slovak Republic Title: Influence of the drone brood homogenate on the gut integrity and cellular immunity: A pilot study on pigs Abstract: Drone brood homogenate (DBH), a nutrient-rich bee product, has received limited scientific attention despite its potential immunomodulatory and gut-protective properties. This study evaluated the effects of a dietary DBH supplementation on the intestinal barrier-related gene expression, phagocytic activity, and lymphocyte subpopulations in pigs. Eighteen weaned pigs were assigned to three groups (control, DBH100, DBH200) and fed DBH at 0, 100, or 200 mg/kg feed for 18 days. The gene expression of tight junction markers (occludin, claudin-1) and mucosal integrity-associated proteins (lumican, OLFM4) was assessed in the ileum by qRT-PCR. Phagocyte function and peripheral blood lymphocyte subpopulations were analysed by flow cytometry. DBH200 significantly upregulated the occludin, claudin-1, lumican, and OLFM4 expression, indicating enhanced intestinal barrier support. The phagocytes from both DBH-treated groups exhibited an increased engulfing capacity and an elevated oxidative burst index, though the percentage of active phagocytes was only weakly affected. The DBH supplementation did not alter the total T (CD3+) or B (CD21+) cells; however, both DBH groups showed a significantly increased CD4+ : CD8+ lymphocyte ratio, which is consistent with immune stimulation. These findings suggest that DBH may beneficially modulate the gut barrier integrity and selected components of innate and adaptive cellular immunity in pigs. Keywords: Apilarnil, gut integrity, lymphocyte subpopulations, phagocytosis, tight junctions Journal: Veterinární medicína Pages: 95-105 Volume: 71 Issue: 3 Year: 2026 DOI: 10.17221/98/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/98/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202603-0004.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:3:id:98-2025-VETMED