Template-Type: ReDIF-Article 1.0 Author-Name: M Falis Author-Workplace-Name: Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: K Benova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: M Spalkova Author-Workplace-Name: Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: L Leskova Author-Workplace-Name: Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Title: Comparison of the effect of Mospilan SP and its active substance on Artemia franciscana Abstract: Contaminants formed after the use of pesticides raise concerns for human health and aquatic ecosystems, i. a., due to their high persistence in surface waters and their further migration to other components of the environment. In our study, we compared the effect of the insecticide Mospilan SP and its active substance acetamiprid on the aquatic crustacean Artemia franciscana. The concentration was 367.44 mg/l for Mospilan SP LC50 after 120 h, and 102.82 mg/l after 144 hours. The concentration was 148.04 mg/l for the active substance acetamiprid LC20 after 144 h and 28.84 mg/l after 168 hours. The commercial insecticide Mospilan SP showed a significant suppressive effect on the measured morphological parameters (body length and body width), with statistically significant differences observed after 10 days of exposure. When comparing the changes after the application of the insecticide and the active substance, it is impossible to confirm the same effect on the morphological parameters within the given time intervals. The objective of the study was to examine morphological changes at sublethal concentrations, as these changes can provide a more sensitive indication of the adverse effects of commercial products and active substances on morphological parameters. Keywords: LC50, morphological changes, neonicotinoids Journal: Veterinární medicína Pages: 201-208 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/73/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/73/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202605-0001.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:5:id:73-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Cahova Author-Workplace-Name: Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: L Kvasnickova Author-Workplace-Name: Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Illek Author-Workplace-Name: Large Animal Clinical Laboratory, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: T Palenik Author-Workplace-Name: Ruminant and Swine Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: A Staffa Author-Workplace-Name: Large Animal Clinical Laboratory, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: V Vlasakova Author-Workplace-Name: State Veterinary Administration Prague, Czech Republic Author-Name: Z Svobodova Author-Workplace-Name: Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: L Ravonjison Author-Workplace-Name: State Veterinary Administration Pardubice, Czech Republic Author-Name: M Svoboda Author-Workplace-Name: Ruminant and Swine Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Title: From grain to forage: A decade of mycotoxin contamination in ruminant feed in the Czech Republic (2013-2023) Abstract: This study is based on analytical data on mycotoxin contamination in ruminant feeds in the Czech Republic over a ten-year period (2013-2023), specifically deoxynivalenol (DON), zearalenone (ZEN), T-2 toxin (T-2/HT-2), and ochratoxin A (OTA). Analyses were performed in accredited State Veterinary Institutes using validated immunochemical and chromatographic methods. Samples included forage and concentrate feeds for dairy cows, calf feed, and fattening cattle feed. During the monitoring period, DON was detected at higher concentrations, reflecting the susceptibility of field crops to Fusarium infection under local environmental conditions. Forage feeds showed significantly higher concentrations of DON (472.6 ± 13.2 µg/kg), ZEN (61.7 ± 4.4 µg/kg), and T-2/HT-2 toxin (36.7 ± 2.7 µg/kg) than concentrates for dairy cows (P < 0.05). Additionally, ZEN levels differed significantly between concentrates for dairy cows (17.1 ± 2.3 µg/kg) and calf feed (16.2 ± 3.3 µg/kg) (P < 0.05). Although mycotoxin concentrations generally remained below recommended limits, a small proportion of samples exceeded guideline values for DON (0.13%) and ZEN (0.56%). These findings highlight the importance of continued monitoring and preventive strategies to manage mycotoxin risks. Keywords: cattle, feed, fungal toxins, occurrence pattern Journal: Veterinární medicína Pages: 180-189 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/74/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/74/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202605-0002.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:5:id:74-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S-H Park Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Buk-gu, Gwangju, Republic of Korea Author-Name: S-W Pak Author-Workplace-Name: Center for Convergence Toxicology Research, Korea Institute of Toxicology, Yuseong-gu, Daejeon, Republic of Korea Author-Name: W-I Kim Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Buk-gu, Gwangju, Republic of Korea Author-Name: B-R Jin Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Buk-gu, Gwangju, Republic of Korea Author-Name: Y-K Cho Author-Workplace-Name: College of Health Sciences, Cheongju University, Sangdang-gu, Cheongju-si, Chungbuk, Republic of Korea Author-Name: T-W Kim Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chungnam National University, Daejeon, Republic of Korea Author-Name: J-W Ko Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chungnam National University, Daejeon, Republic of Korea Author-Name: J-S Kim Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Buk-gu, Gwangju, Republic of Korea Author-Name: J-C Kim Author-Workplace-Name: College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Buk-gu, Gwangju, Republic of Korea Author-Name: AY Lee Author-Workplace-Name: KM Data Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea Author-Name: I-S Shin Author-Workplace-Name: Center for Convergence Toxicology Research, Korea Institute of Toxicology, Yuseong-gu, Daejeon, Republic of Korea Title: Protective effect of Loranthus tanakae Franch. & Sav. on allergic asthma induced by ovalbumin Abstract:  Allergic asthma is a widespread disease with elevated eosinophil levels. Although corticosteroids are widely prescribed for allergic asthma, numerous patients experience limited sensitivity and side effects. Loranthus tanakae Franch. & Sav., a traditional herbal plant, has anti-inflammatory and antioxidant properties in pulmonary inflammation caused by Asian sand dust and cigarette smoke condensate. To assess the protective effects of L. tanakae, we examined the influence of an L. tanakae ethanol extract (LTE) in ovalbumin (OVA)-induced asthma. Mice received intraperitoneal sensitisation with OVA, and challenged using OVA inhalation. LTE was consecutively orally gavaged for 6 days. Following sacrifice, the bronchoalveolar lavage fluid (BALF) and lung tissue was analysed. The LTE treatment considerably reduced inflammatory cell counts, proinflammatory cytokine levels in the BALF, and immunoglobulin E, compared with the OVA group, along with a reduction in airway hyperresponsiveness. The LTE also improved airway inflammation and suppressed mucus hypersecretion in the lung tissues. Additionally, the expression of MMP-9 and activation of ERK, JNK, and p38 were notably diminished in the LTE groups. This study revealed reduced airway inflammation in OVA-induced asthma via suppressing the MMP-9 and mitogen-activated protein kinase-associated factors. Consequently, our findings demonstrated that LTE is suggested as a potential remedy for allergic asthma. Keywords: airway inflammation, herbal medicine, immune disease, matrix metalloproteinase-9 Journal: Veterinární medicína Pages: 190-200 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/77/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/77/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202605-0003.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:5:id:77-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I Szabo Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: L Sabova Author-Workplace-Name: Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: R Sabo Author-Workplace-Name: Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: M Sucik Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Title: Drugs and products in beekeeping: Efficacy and perspectives of natural alternatives Abstract: Honeybee health is a key factor in sustainable apiculture, with the most significant colony losses attributed to the mite Varroa destructor and microsporidia of the genus Nosema spp. The aim of this review article is to summarise current knowledge on the use of pharmaceuticals and preparations in the prevention and treatment of these diseases and to highlight promising alternatives to synthetic acaricides. Commonly used active substances such as amitraz and pyrethroids show decreased efficacy in many countries due to the development of resistance. Therefore, organic acids (formic, oxalic, and lactic), essential oils, and plant extracts are increasingly used as environmentally friendly, residue-free alternatives. Some plant preparations and extracts have been shown to be effective against Nosema ceranae. In addition to therapeutic interventions, the importance of preventive measures and immune support through supplements containing vitamins, amino acids, and probiotics is emphasised. The article highlights the need for integrated bee health management combining pharmacological, natural, and biotechnological approaches to limit resistance development, minimise residues in bee products, and promote overall colony vitality. Keywords: integrated bee protection, Nosema ceranae, organic acids, plant extracts, veterinary preparations, Varroa destructor Journal: Veterinární medicína Pages: 169-179 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/88/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/88/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202605-0004.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:5:id:88-2025-VETMED