Template-Type: ReDIF-Article 1.0 Author-Name: AO Sanni Author-Workplace-Name: Department of Veterinary Tropical Diseases, University of Pretoria, Onderstepoort, South Africa Author-Workplace-Name: Agro-Processing, Productivity Enhancement and Livelihood Improvement Support (APPEALS) Project, Lokoja, Kogi State, Nigeria Author-Name: AH Jibril Author-Workplace-Name: Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Sokoto State, Nigeria Author-Name: A Jonker Author-Workplace-Name: Department of Veterinary Tropical Diseases, University of Pretoria, Onderstepoort, South Africa Author-Name: FO Fasina Author-Workplace-Name: Department of Veterinary Tropical Diseases, University of Pretoria, Onderstepoort, South Africa Author-Workplace-Name: Department of Agriculture and Animal Health, College of Agriculture and Environmental Sciences, University of South Africa (UNISA), Florida Park, Roodepoort, South Africa Title: Salmonella enterica isolates from poultry in North-Central Nigeria reveal multidrug-resistant patterns and risk of contamination to the human food chain Abstract: Non-typhoidal (NT) salmonellosis is a major poultry-associated bacterial zoonosis. We undertook environmental sampling (poultry faeces and dust, n = 600) to evaluate the prevalence of NT Salmonella in poultry farms in North-Central Nigeria (NCN). Samples from five NCN states and Abuja were processed using standard bacterial culture and the invA-based PCR method, and isolates were tested against the 11 most used poultry antimicrobials by means of the Kirby-Bauer disk diffusion method. We measured inhibition zones using WHONET to classify resistance patterns. The overall prevalence rate was 18.7%, and prevalence rates in dust and faeces were 20.5% and 17.1%, respectively. Prevalence was lower in battery-caged birds, in flocks >1 000 birds, in older birds (>52 weeks, one year) and in layer farms. The odds of infection with NT Salmonella was ≥ twofold in younger birds. Isolates were most resistant to tetracycline (73.8%), nalidixic acid (59.5%) and sulphonamides (54.8%) and were most susceptible to ceftazidime (88.1%) and cefotaxime (78.6%). Single drug-resistant, multidrug-resistant, extensively drug-resistant and pandrug-resistant isolates were obtained, indicating abuse of antimicrobials in the poultry industry. Stricter control of access to antimicrobials will mitigate antimicrobial resistant pathogens, and inaction will complicate antimicrobial resistance in the human food chain with subsequent health and economic implications. Keywords: antibacterial susceptibility, environmental contamination, food safety, livestock pathogens, zoonotic bacteria Journal: Veterinární medicína Pages: 422-433 Volume: 71 Issue: 10 Year: 2026 DOI: 10.17221/76/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/76/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202610-0001.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:10:id:76-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y Mesfer Alharbi Author-Workplace-Name: Department of Medical Biosciences, College of Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia Author-Name: A Ibraheem Alnuqaydan Author-Workplace-Name: Zoo Supervisor, King Khalid Military City Under the Ministry of Defense, Hafar Al-Batin, Saudi Arabia Author-Name: AKA Zaki Author-Workplace-Name: Department of Medical Biosciences, College of Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia Author-Workplace-Name: Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza Square, Egypt Title: The impact of 5% flaxseed supplementation on sex hormones, antioxidant status, and blood profile in female spotted deer during the breeding season Abstract: Phytoestrogens, such as those found in flaxseed, can mimic oestrogen and influence animal physiology. For spotted deer, an economically significant species in Saudi Arabia, information on how phytoestrogen-rich diets affect reproductive health and overall physiology is limited, particularly in captive zoo settings. This work aimed to clarify the effects of a 5% flaxseed dietary supplement on the sex hormone profile, antioxidant capacity, haematological parameters, and histological features of female spotted deer during the onset of the breeding season. Twelve female deer were separated into two groups: a control group fed a standard diet of alfalfa and pellets, and an experimental group receiving the same diet supplemented with 5% flaxseed for six weeks. Weekly measurements included body weight and feed intake. Blood samples were analysed for sex hormones (oestradiol, testosterone), sex hormone-binding globulin (SHBG), antioxidant enzymes, oxidative stress markers, complete blood count (CBC), and biochemical parameters. Ovarian and reproductive tract histology was also examined. Flaxseed supplementation significantly increased serum oestradiol (E2) levels and SHBG (P < 0.05), reducing the Free Androgen Index (FAI). It also significantly enhanced antioxidant enzyme activities (SOD and CAT). Haematologically, it increased red blood cell count but induced microcytic hypochromic erythropoiesis, suggesting possible iron chelation. A notable increase in eosinophils was observed. Histologically, reproductive tissues showed enhanced development in the flaxseed group. Incorporating 5% flaxseed into the diet of female spotted deer induces a significant oestrogenic shift, improves antioxidant defence, and enhances reproductive tissue morphology. However, it may also cause iron deficiency-like haematological changes and a potential allergic response. This suggests that flaxseed is a potent dietary tool for modulating reproduction in captive deer, but its application requires careful management to mitigate adverse effects. Keywords: antioxidants, Axis axis, captive wildlife, erythropoiesis, lignans, oestradiol, phytoestrogens Journal: Veterinární medicína Pages: 399-414 Volume: 71 Issue: 10 Year: 2026 DOI: 10.17221/78/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/78/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202610-0002.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:10:id:78-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S-Y Park Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine & Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu, Republic of Korea Author-Name: W-K Yoon Author-Workplace-Name: Guardian Angel Veterinary Hospital, Anyang, Republic of Korea Author-Name: Y Oh Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine & Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu, Republic of Korea Title: Heartworm removal using the basket catheter technique in a 1.85 kg cat with pulmonary hypertension and right-sided heart failure: A case report Abstract: Feline heartworm infection poses significant diagnostic and therapeutic challenges owing to its distinct clinical features compared to canine cases. Even a single worm can cause life-threatening complications, including pulmonary hypertension and right-sided heart failure. There is no definitive treatment available at present. A 5-month-old intact male domestic shorthair cat (1.85 kg) exhibited respiratory distress, pleural effusion and ascites. Clinical examination revealed a grade 4/6 systolic murmur, jugular vein distension and 7% dehydration. Despite negative antigen and antibody tests for heartworm, echocardiography revealed hyperechoic structures in the right atrium consistent with Dirofilaria immitis, alongside pulmonary hypertension and right-sided heart failure. A 10-cm-long immature female heartworm was surgically removed through jugular venotomy using a basket device under fluoroscopic guidance. Postoperatively, the cat developed persistent pulmonary hypertension, tricuspid regurgitation and refractory hypotension, leading to death on day five. Necropsy confirmed no residual heartworms and showed eosinophilic arteritis, pulmonary oedema, alveolar histiocytosis and thrombus formation, all of which are consistent with heartworm-associated pulmonary vascular disease. Although basket device-assisted removal is feasible, existing vascular damage and postoperative complications significantly affect prognosis. This case report describes a patient managed for clinical diagnostic and therapeutic purposes. All procedures were performed as part of standard veterinary clinical care, and informed consent for publication was obtained from the rescuer. Since this report did not involve experimental interventions, approval from an institutional animal ethics committee was unnecessary. Keywords: Dirofilaria immitis, feline heartworm disease, heartworm-associated respiratory disease, surgical removal Journal: Veterinární medicína Pages: 434-439 Volume: 71 Issue: 10 Year: 2026 DOI: 10.17221/91/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/91/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202610-0003.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:10:id:91-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L Culka Author-Workplace-Name: Clinic for Swine, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: J Novotny Author-Workplace-Name: Clinic for Swine, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: L Leskova Author-Workplace-Name: Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: Z Krepelkova Author-Workplace-Name: Clinic for Swine, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: V Karaffova Author-Workplace-Name: Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: M Madarova Author-Workplace-Name: Clinic for Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: P Schusterova Author-Workplace-Name: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Title: Impact of preanalytical sample handling on GLP-1 measurement in porcine plasma: A pilot ELISA-based study Abstract: Glucagon-like peptide-1 (GLP-1) is an incretin hormone that plays a key role in the regulation of glucose metabolism; however, it is highly sensitive to biological sample handling conditions. The aim of this pilot study, conducted in two piglets and two adult finishing pigs, was to compare the effects of selected preanalytical factors on measured GLP-1 concentrations in porcine plasma and to identify conditions suitable for its reliable determination using ELISA. The evaluated factors included blood collection tube type (EDTA vs EDTA with a DPP-4 inhibitor), centrifugation temperature (4 °C vs room temperature), and sample processing conditions (fresh vs frozen samples). In addition, the influence of the physiological status of the animals (fasting vs post-glucose administration) was assessed. Samples collected in tubes containing a DPP-4 inhibitor showed higher measured GLP-1 concentrations than standard EDTA samples. Samples processed at room temperature showed higher GLP-1 concentrations compared to samples centrifuged at 4 °C. The obtained results suggest that sample handling conditions may significantly influence GLP-1 determination by ELISA. The use of EDTA tubes containing a DPP-4 inhibitor combined with rapid sample processing and centrifugation at 4 °C may represent a suitable procedure for standardised GLP-1 measurement. Keywords: DPP-4 inhibitor, ELISA, GLP-1, porcine plasma, sample handling, sample stability Journal: Veterinární medicína Pages: 415-421 Volume: 71 Issue: 10 Year: 2026 DOI: 10.17221/34/2026-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/34/2026-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202610-0004.txt Handle: RePEc:caa:jnlvet:v:71:y:2026:i:10:id:34-2026-VETMED