Czech J. Food Sci., 2004, 22(10):S199-S202 | DOI: 10.17221/10659-CJFS
Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants
- 1 Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic
- 2 Department of Chemistry of Natural Substances, Institute of Chemical Technology, Prague, Czech Republic
The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4' via a carboxy methyl spacer. The developed ELISAs are highly specific, I50 values of the standard curves range between 0.3-1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid molecules is negligible. The immunoassays have been used for monitoring of changes in isoflavone levels in alimentary important plants, such as Medicago sativa, during germination; and during different vegetation stages of the Rutaceae family plants.
Keywords: ELISA; isoflavone; daidzein; genistein; biochanin A; Ruta; Citrus
Published: December 31, 2004 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.