Czech J. Food Sci., 2009, 27(10):S102-S104 | DOI: 10.17221/944-CJFS

Determination of Lactoferrin in Goat Milk by HPLC Method

M. Dračková*, I. Borkovcová, B. Janštová, M. Naiserová, H. Přidalová, P. Navrátilová, L. Vorlová
Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, 612 42 Brno, Czech Republic, *E-mail: drackovam@vfu.cz

The aim of this study was the determination of lactoferrin in goat milk using HPLC method. Milk samples were collected at a goat farm in the South Moravia Region, the Czech Republic. It were established bulk tank samples of raw milk (n = 24) and pasteurised milk (nn = 27) that were collected during lactation. Lactoferrin contents were analysed by reverse phase high-performance liquid chromatography (RP-HPLC) with diode-array detector PDA 2996. Detection was carried out at the wavelength 205 nm. The average concentration of lactoferrin in goat milk was 120 ± 18 μg/ml. The lactoferrin content was increasing within the lactation period in the ranges of 98 ± 170 μg/ml in April to 149 ± 19 μg/ml in November. The heat treatment (pasteurisation at 72°C for 20 s) resulted in no significant effect on the lactoferrin content. No statistically significant differences (P = 0.05) were found between the values of raw and pasteurised goat milk.

Keywords: lactoferrin; milk; RP HPLC; goat

Published: June 30, 2009  Show citation

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Dračková M, Borkovcová I, Janštová B, Naiserová M, Přidalová H, Navrátilová P, Vorlová L. Determination of Lactoferrin in Goat Milk by HPLC Method. Czech J. Food Sci. 2009;27(Special Issue 1):S102-104. doi: 10.17221/944-CJFS.
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