Czech J. Food Sci., 2009, 27(10):S256-S258 | DOI: 10.17221/1097-CJFS

Anticancer Activity of Lignan from the Aerial Parts of Saussurea salicifolia (L.) DC.

G. Chunsriimyatav^*,1,2, I. Hoza¹, P. Valášek¹, S. Skrovanková¹, D. Banzragch², N. Tsevegsuren³

¹ Department of Food Engineering, Tomas Bata University in Zlin, 760 01 Zlín, Czech Republic

² Institute of Chemistry and Chemical Technology, Mongolian Academy Sciences, Ulaanbaatar, MON 51 Mongolia

³ Department of Organic Chemistry, Faculty of Chemistry, National University of Mongolia, Ulaanbaatar, Mongolia, *E-mail: chunsrii82@yahoo.com$2

Aerial parts of Saussurea salicifolia (L.) DC were studied for their lignan and flavonoids in solvent chloroform and n-butanol of ethanolic extract. Isolation and identification of phenolic compounds of the chloroform and n-butanol fractions were performed with Dionex HPLC-DAD system with water-methanol gradients in 4 different wave lengths (235 nm, 254 nm, 280 nm and 340 nm), using online UV and LC-MS as described previously. 9-OH-pinoresinol which is a lignan with anticancer activity was dominated in the chloroform fraction, whereas mainly flavonoid glycosides like quercetin-3-O-galactoside, apigenin-7-O-rhamnoside with anti-inflammatory effect were detected in the n-butanol fraction. Additionally, 9-OH-pinoresinol was also found in the n-butanol fraction. Anticancer tests were conducted in leukemia mouse lymphoma cells L5178Y at a concentration of 10 μg/ml of test compound. Crude ethanol extract of S. salicifolia reduced the growth of leukemia mouse lymphoma cells L5178Y to 23.8%.

Keywords: flavonoids; Saussurea salicifolia; anticancer activity; Dionex HPLC-DAD system

Published: June 30, 2009  Show citation