Czech J. Food Sci., 2009, 27(10):S366-S368 | DOI: 10.17221/603-CJFS

Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation

M. Du¹ková1, A. ©panová2, V. Dráb3, B. Rittich2*
1 Department of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences Brno, 612 42 Brno, Czech Republic
2 Institute of Experimental Biology, Masaryk University, 602 00 Brno, Czech Republic
3 Dairy Research Institute Ltd., 160 00 Praha, Czech Republic

In this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as a DNA probe for the screening of homologous DNA sequences in 7 Lactococcus lactis subsp. lactis strains from the Culture Collection of Dairy Microorganisms (CCDM). Dot blot hybridisation and hybridisation of colonies were used for DNA/DNA hybridisation. It was shown that 6 tested strains of Lactococcus lactis subsp. lactis have genes encoding nisin. One strain had probably a defective gene encoding nisin.

Keywords: Lactococcus lactis subsp .lactis; screening; nisin; gene; PCR probe; DNA, DNA hybridisation

Published: June 30, 2009  Show citation

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Du¹ková M, ©panová A, Dráb V, Rittich B. Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation. Czech J. Food Sci. 2009;27(Special Issue 1):S366-368. doi: 10.17221/603-CJFS.
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