Czech J. Food Sci., 2009, 27(10):S369-S371 | DOI: 10.17221/945-CJFS

Methods for the Determination of Allergenic Substances in Foods

K. Tomková1, F. ©tumr1, P. Dvorská1, P. ©afáøová1, J. Rysová2, D. Gabrovská2*, P. Hanák2, J. Plicka3
1 SEDIUM RD, s.r.o., 530 02 Pardubice, Czech Republic
2 Food Research Institute Prague, 102 31 Prague, Czech Republic
3 Immunotech a Beckman Coulter Company, 102 27 Prague, Czech Republic

Within the framework of the research project ELISA methods for the quantitative determination of allergenic substances in foodstuff and raw materials were developed. ELISA kits for allergenic proteins of milk (casein, beta-lactoglobulin and BSA) egg white proteins and mustard proteins were validated and collaborative studies were performed to prove the validation of the ELISA methods developed. Various methods of extraction were tested. The parameters as a limit of detection, as a limit of quantification, robustness, repeatability and accuracy were determined. A broad range of zero matrices for allergens were tested as well. The ELISA kits are suitable for the determination of allergens according to EU legislation Directive 2005/26/EC and Directive 2006/142/EC in the laboratories focused on this topic.

Keywords: food allergy; ELISA methods; allergenic protein determination

Published: June 30, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Tomková K, ©tumr F, Dvorská P, ©afáøová P, Rysová J, Gabrovská D, et al.. Methods for the Determination of Allergenic Substances in Foods. Czech J. Food Sci. 2009;27(Special Issue 1):S369-371. doi: 10.17221/945-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anonymous (2004): Opinion of the Scientific Panel on Dietetic products, nutrition and allergies [NDA] on a request from the Commission relating to the evaluation of allergenic foods for labelling purposes. The EFSA Journal, 32: 1-197.
    2. Directive 2005/26/EC of the European Parliament and of the Council of 21 March 2005.
    3. Directive 2006/142/EC of the European Parliament and of the Council of 22 December 2006.
    4. Decree No. 101/2007 Coll. (the Czech Republic), on the method of labelling food and tobacco products. Date of entry into force: 12 April 2005.
    5. Sathe S.K., Teuber S.S., Roux K.H. (2005): Effects of food processing on the stability of food allergens. Biotechnology Advances, 23: 423-426. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content