Czech J. Food Sci., 2009, 27(10):S417-S420 | DOI: 10.17221/932-CJFS

Analysis of Bread Lipids for 3-MCPD Esters

M. Doležal^*, J. Kertisová, Z. Zelinková, J. Velíšek

Department of Food Chemistry and Analysis, Institute of Chemical Technology in Prague, Czech Republic

Objective of this work was determination of processing contaminant known as 3-MCPD (3-chloropropane-1,2-diol) in its free and bound form in breads with defined parameters of processing. Selected and analysed were 24 samples, which represented two sets of breads produced in bakeries equipped with a continual line. In all cases determinations were carried out for breadcrumb and crust separately. The first set of samples were wheat-rye breads produced chronologically in ten days in the bakery Michelská pekárna, slightly different in temperatures and times of baking. The second set contained 14 samples of wheat-rye breads with a content of rye flour less than 40% differing in the yeast type and acidity. These breads were produced in the bakery Kontinua. The fat content was determined in all samples by Soxhlet extraction. Free and bound 3-MCPD was determined by gas chromatography-mass spectrometry method. Concentration of free 3-MCPD in samples was at interval < 9-54.5 μg/kg. Concentration of bound 3-MCPD was at interval 1.56-23.60 mg/kg of fat (i.e. 5.7-84.9 μg/kg of sample).

Keywords: bread; 3-chloropropane-1,2-diol; 3-MCPD; 3-MCPD esters; contaminants

Published: June 30, 2009  Show citation