Plant Protect. Sci., 2019, 55(2):134-141 | DOI: 10.17221/119/2018-PPS

Virulence of new strain of Heterorhabditis bacteriophora from Croatia against Lasioptera rubiOriginal Paper

Ivana Majić1*, Ankica Sarajlić1, Tamás Lakatos2, Tímea Tóth2, Emilija Raspudić1, Zlatko Puškadija1, Gabriella Kanižai Šarić1, Žiga Laznik3
1 Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
2 National Agricultural Research and Innovation Centre, Fruitculture Research Institute, Ujfeherto, Hungary
3 Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

Soil samples from 100 cultivated and natural sites were assessed for the presence of entomopathogenic nematodes. Heterorhabditid nematodes were recovered from three soil samples during spring months, with the overall positive sample rate of 3%. The isolates of entomopathogenic nematodes were identified as three different strains conspecific with Heterorhabditis bacteriophora (Heterorhabditidae). They were found from natural sites and vineyard, while no recovery occurred from intensively cultivated agricultural fields. The morphometrical characteristics of infective juveniles and males showed differences between all Croatian strains and from the original description. Heterorhabditis bacteriophora ISO9 was bioassayed on Lasioptera rubi (Cecidomyiidae) (the raspberry gall midge) larvae at different nematode concentrations under laboratory conditions. The significantly highest mortality was observed in treatments with 50 and 200 infective juveniles per insect larvae within 8 days after inoculation. This is the first report of entomopathogenic nematodes of the family Heterorhabditidae from Croatia, and susceptibility of L. rubi larvae to entomopathogenic nematodes. The Croatian strain H. bacteriophora ISO9 was proved to possess strong insecticidal properties against L. rubi larvae.

Keywords: entomopathogenic nematode; raspberry gall midge; bioassay; biocontrol

Published: June 30, 2019  Show citation

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Majić I, Sarajlić A, Lakatos T, Tóth T, Raspudić E, Puškadija Z, et al.. Virulence of new strain of Heterorhabditis bacteriophora from Croatia against Lasioptera rubi. Plant Protect. Sci. 2019;55(2):134-141. doi: 10.17221/119/2018-PPS.
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