Plant Protect. Sci., 2023, 59(3):278-283 | DOI: 10.17221/1/2023-PPS

Culture of Steinernema glaseri on three solid media and their virulence against Galleria mellonella larvaeOriginal Paper

Carlos I. Cortés-Martínez ORCID...1, Adriana Ines Rodríguez-Hernández2, Ma del Rocío López-Cuellar2, Norberto Chavarría-Hernández2, Rodolfo De los Santos Romero ORCID...3
1 Tecnológico Nacional de México, Instituto Tecnológico del Valle de Etla, Oaxaca, México
2 Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma de Hidalgo, Hidalgo, México
3 Tecnológico Nacional de México, Instituto Tecnológico del Valle de Oaxaca, Oaxaca, México

Steinernema glaseri is a potential biocontrol agent against white grubs of the Phyllophaga spp. complex; however, its suitability for in vitro multiplication has been scarcely investigated. In this study, the effects of the surface culture of NJ-43 strain with its symbiotic bacteria Xenorhabdus poinarii on egg-yolk agar (P2), chicken-liver agar and nutrient meat-peptone (MP) agar on infective juvenile (IJ) productivity and their virulence against Galleria mellonella larvae were investigated. The bacteria on the surface of the agar were first incubated in darkness at 30 °C for 54–102 h, and then 100 surface-sterilised IJs were added. After two harvests, the accumulated productivity was higher on chicken-liver agar (536 × 103 IJs/m2 day) and P2 agar (534 × 103 IJs/m2 day) than on nutrient MP agar (58 × 103 IJs/m2 day). The mean virulence of the in vitro produced IJs was 46–60% and showed no statistically significant difference among the three culture media. In conclusion, the maximum multiplication factor of S. glaseri NJ-43 on solid media was 385, and its original virulence was retained.

Keywords: biocontrol, entomopathogenic nematodes, in-vitro production, one-on-one assay

Received: January 3, 2023; Revised: April 18, 2023; Accepted: May 29, 2023; Prepublished online: August 7, 2023; Published: September 20, 2023  Show citation

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Cortés-Martínez CI, Rodríguez-Hernández AI, del Rocío López-Cuellar M, Chavarría-Hernández N, De los Santos Romero R. Culture of Steinernema glaseri on three solid media and their virulence against Galleria mellonella larvae. Plant Protect. Sci. 2023;59(3):278-283. doi: 10.17221/1/2023-PPS.
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