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Rabbit and quail: Little known but valuable meat sourcesReviewAntonella Dalle Zotte, Marco CullereCzech J. Anim. Sci., 2024, 69(2):39-47 | DOI: 10.17221/165/2023-CJAS In the actual scenario of increasing demand of animal food and the need to develop sustainable production systems, rabbit (Oryctolagus cuniculus) and quail (Coturnix japonica) represent animal species of undeniable potential, profoundly linked to human’s eating habits in several parts of the World, yet not fully exploited. Given the remarkable adaptability of quail and rabbit to different farming contexts, they can be successfully farmed both in specialised and technologically advanced production systems targeting market diversification, as well as in such areas of the World where malnutrition is a severe issue and technological limitations are present. Together with the above-mentioned socio-cultural aspects, rabbit and quail possess intrinsic traits that make them meat species of interest to improve the sustainability of the livestock sector, challenge of utmost importance for present and future development strategies. Furthermore, available nutritional data indicate that quail and rabbit provide food (meat) that has the potential to improve the nutritional adequacy of any diet. It also emerged that about 130 g of rabbit and quail meat are sufficient to meet the daily dietary recommendations for essential amino acids provided by the World Health Organization. Despite the dietary quality of the meat obtained from these two animal species, the present review also unveils that, while the factors affecting meat quality have been extensively studied, little focus has been devoted on those specifically affecting the protein content and quality, thus emphasising that this is a key aspect for future researches. |
Effects of plant sterol microcapsules on growth performance and serum biochemical indicators in pigsOriginal PaperBin Song, Shengwei Di, Yan Zhao, Ziwei Li, Junjie Jin, Fengxiang Hou, Yawen Zhang, Hongxi XuCzech J. Anim. Sci., 2023, 68(12):497-507 | DOI: 10.17221/64/2023-CJAS To better explore the effects of adding phytosterol (PS) microcapsules to feed on pig growth performance, nutrient apparent digestibility, and serum biochemical indicators, 200 healthy ternary hybrid Duroc × Landrace × Yorkshire piglets with an average initial weight of 7.53 ± 0.57 kg) were used as experimental subjects, and randomly divided into five groups with four replicates in each group. The control group of pigs was fed the basic diet, while the experimental group received diets supplemented with different PS levels. They were divided into experimental groups 1 to 4 (100 mg/kg to 5 502 mg/kg) according to different dosage added components. The duration of the experiment was 54 days. The results showed that the total triglyceride (TG) index and diarrhoea status in the experimental group were significantly improved compared to the control group (P < 0.05). The increase in PS addition levels was more significant in the change of pig average daily gain (ADG) (P < 0.05), and the difference in average daily weight gain and feed conversion ratio between PS-400 and PS-550 was significant (P < 0.05). Compared with the control group, the experimental group showed significant differences in crude fibre (CF), ether extract (EE), dry matter (DM), organic matter (OM), average digestible energy (ADE), metabolizable energy, xylan, and calcium (P < 0.05), with a maximum increase of 7.02% in calcium content. The addition of PS can be effective in making the experimental and control groups show a significant difference in value changes in high-density cholesterol (HDL-C) and low-density cholesterol (LDL-C) indicators (P < 0.05), while no significant changes were revealed in the other indicators (P > 0.05). From the above, plant sterol microcapsules can effectively improve pig growth performance and nutrient apparent digestibility, and improve their blood lipid status. |
Genomic characterization of Serbian Holstein-Friesian cattle populationOriginal PaperMomèilo ©aran, Ljuba ©trbac, Dobrila Jankoviæ, Mihajla Djan, Sne¾ana Trivunoviæ, Minja ZorcCzech J. Anim. Sci., 2023, 68(12):486-496 | DOI: 10.17221/89/2023-CJAS The use of genomic data makes it possible to examine genetic variability and calculate the genetic parameters of the population in an efficient and precise way. The aim of this research was to analyse linkage disequilibrium (LD), contemporary effective population size (Nec), haplotype block structure, minor allele frequency (MAF), observed (HO) and expected heterozygosity (HE), calculate the genomic relationship matrix and perform a principal component analysis (PCA) in the Serbian Holstein-Friesian cattle population using SNP data from the GGP Bovine 100K chip. After quality control (QC), 83 208 SNPs and 1 575 cows were retained for further analysis. LD on autosomes had an average value of ≥ 0.2 up to a distance of 50–60 kb (r2 = 0.211), while on BTX r2 ≥ 0.2 was represented at distances of 80–90 kb (r2 = 0.211). LD differed between chromosomes. The average HO for autosomes and X chromosome SNPs was 0.412 and 0.422, respectively. 81.30% of SNPs that passed QC had MAF > 0.2. The total number of haplotype blocks in the studied population was 15 642. On average, blocks contained 2.932 SNPs. The average block length was 32.657 kb and ranged from a minimum of 0.019 kb (BTA21 and BTA26) to a maximum of 999.562 kb (BTX). The estimated value of Nec in the this cattle population was 142. The results of PCA showed a significant variability of genotypes in the population, but there was no clear stratification of the population. The obtained results will serve as a basis for future genomic analyses such as the detection of QTLs for important economic traits and the implementation of genomic selection. |
Effects of age and litter-of-origin on cryopreserved spermatozoa in Sumava ramsOriginal PaperAne¾ka Málková, Martin Ptáèek, Filipp Georgijeviè Savvulidi, Szabolcs Tamás Nagy, Ludìk StádníkCzech J. Anim. Sci., 2024, 69(4):129-138 | DOI: 10.17221/32/2024-CJAS This study aimed to evaluate the influence of the internal factors of ram age and litter-of-origin on semen quality during the cryopreservation process in Sumava sheep rams. This breed is included in the protected genetic resources of the Czech Republic. The sires were systematically divided into four groups according to age (under 1.5 years, 1.5 to 2.5 years, 2.5 to 3.5 years, and over 4.5 years) and according to litter frequency (singletons vs twins). Semen was evaluated after equilibration, and after cryopreservation using iSperm® mCASA and flow cytometry. During cryopreservation, there was a significant decrease in total sperm motility by 53.5%, progressive motility by 38%, and cells with intact plasma membrane and acrosome by 47%. Frozen-thawed sperm kinematic parameters showed significant age-related variations, with rams aged 4.5 and older displaying notably higher total and progressive motility (16.2% and 6.24%, respectively). Rams born as twins exhibited 3.77% (P < 0.05) higher progressive motility and 5.5% (P < 0.05) higher total motility compared to those born as singles. The sperm of older rams (> 4.5 years) exhibited higher viability (10.1%) and lower damage to the plasma membrane after freeze-thawing (23.6%), (P < 0.05). Twins showed significantly higher sperm viability (4.98%, P < 0.05) than singletons. These rams produce a larger quantity of higher-quality insemination doses after cryopreservation. For Sumava rams, in particular, broadening the sire selection base helps to select suitable rams for breeding. As the sire ages, his genetic value within the production herd may decline with time. This contrasts with genetic resource protection, where the aim is to preserve and store as many high-quality semen samples as possible. |
Promotional effects on naturally occurring lactic acid bacteria without impairing chickpea germinationOriginal PaperEli¹ka Kováøíková, Veit Ny, Miloslav ©ulc, Jana Rysová, Natálie Peèenková, Milan Hou¹kaCzech J. Food Sci., 2024, 42(2):85-92 | DOI: 10.17221/12/2024-CJFS
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Effects of foliar application of amino acid-chelated selenite on photosynthetic characteristics of peanut (Arachis hypogaea L.) leaves at the podding stageOriginal PaperYing Wang, Qing Zhu, Zhiwei Wang, Junpei Wang, Zhen Wang, Feiyan Yu, Lianhe ZhangPlant Soil Environ., 2024, 70(1):17-25 | DOI: 10.17221/406/2023-PSE Foliar application of selenium (Se) is an effective measure to increase Se concentrations in peanut pods. However, how the foliar application of amino acid-chelated selenite affects the photosynthetic characteristics of peanut leaves at the podding stage is still unclear. Here, the effects of Se on the activities of antioxidant enzymes, the concentrations of chlorophyll, soluble protein, soluble sugar, and reduced glutathione (GSH), photosynthetic parameters, and Se concentration of peanut leaves were investigated by spraying selenite, L-lysine-chelated selenite, and amino acid-chelated selenite solutions, respectively. The results indicated that foliar application of Se could significantly increase leaf Se concentration. The net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr) of leaves were significantly higher than those in the control. However, peanut leaves’ intercellular CO2 concentration (ci) decreased significantly. Further study found that the concentrations of chlorophyll, soluble protein, soluble sugar, and GSH in peanut leaves increased significantly, and the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in peanut leaves were significantly higher than those in control. However, there were no significant differences between the foliar application of selenite, L-lysine-chelated selenite, and amino acid-chelated selenite. Thus, foliar application of selenite, L-lysine-chelated selenite, and amino acid-chelated selenite could effectively enhance the photosynthetic functions of peanut leaves, which was closely associated with the improvement of antioxidant enzyme activities and the concentrations of soluble sugar, soluble protein, and GSH, resulting in inhibiting chlorophyll degradation and improving the photosynthetic functions of peanut leaves. |
Responses of soil nutrients, enzyme activities, and maize yield to straw and plastic film mulching in coastal saline-alkalineOriginal PaperZhen Liu, Mengkun Zhang, Zengjiao Wang, Ying Shen, Deheng Zhang, Shenghao Zhang, Xingchao Qi, Xuepeng Zhang, Tao Sun, Shenzhong Tian, Tangyuan NingPlant Soil Environ., 2024, 70(1):40-47 | DOI: 10.17221/284/2023-PSE To address the issue of low soil nutrients and low crop yields in coastal alkaline salines, a field experiment of straw combined with plastic film mulching in coastal alkaline salines was conducted in this study to explore the effects of different treatments on soil nutrients, enzyme activities and maize yield. Four treatments, including no mulching (NM), straw mulching (SM), plastic film mulching (PM), and straw mulching combined with plastic film mulching (SP), were set up during 2019–2020. In the 0–20 cm soil layer, compared with NM, the soil organic carbon (SOC) and soil catalase activity (SCA) of SM significantly increased by 23.4% and 46.2%, respectively (P < 0.05). The soil total nitrogen (STN), soil available phosphorus (SAP), available potassium (SAK), sucrase activity, urease activity, alkaline phosphatase activity, and maize yield (MY) of SP significantly increased by 40.7, 26.8, 13.9, 34.6, 73.8, 36.2 and 19.0%, respectively (P < 0.05). SOC, STN, SAP, SAK and SCA were significantly correlated with MY. Therefore, straw mulching combined with plastic film mulching has the best effect on increasing soil nutrients, soil enzyme activity, and maize yield and is suitable for promotion and application in coastal alkaline salines. |
The effects of diverse microbial community structures, driven by arbuscular mycorrhizal fungi inoculation, on carbon release from a paddy fieldOriginal PaperXue Zhang, Xiaodong Yu, Yunxiao Cao, Jiani Yue, Shan Wang, Yunxia LiuPlant Soil Environ., 2024, 70(1):48-59 | DOI: 10.17221/340/2023-PSE Arbuscular mycorrhizal fungi (AMF) play a key role in regulating the carbon cycle in terrestrial ecosystems. However, there is little information on how AMF inoculation affects the carbon fluxes of paddy fields, which are major sources of global carbon emissions. We, therefore, designed an experiment to study the effects of AMF inoculation on methane and carbon dioxide emissions from a paddy field. Results showed that: (1) Among the tested factors, the C/N ratio was the main environmental determinant of microbial community structure in the investigated soil; (2) compared with traditional fertilisation (control), the soil C/N ratio increased by 2.1~15.2% and 1.4~10.5% as a result of AMF application alone (M) or in combination with mineral fertiliser (FM) throughout the growing season, respectively. This change shifted microbial community composition to higher G+/G− bacterial and fungal/bacterial ratios; (3) the microbial community change favoured soil carbon retention. Methane (CH4) emission peaks were reduced by 59.4% and 76.0% versus control in the M treatment and by 52.5% and 29.4% in the FM treatment in the midseason and end-of-season drainage periods, and CO2 emission peaks were reduced by 70.1% and 52.3% in the M plots and by 55.4% and 66.4% in the FM plots. |
Protective effects of green tea (Camellia sinensis) extract against cypermethrin-induced neurotoxicity in rainbow trout (Oncorhynchus mykiss) brain tissuesOriginal PaperMurteza Cakir, Tayfun Karatas, Serkan YildirimCzech J. Anim. Sci., 2024, 69(1):29-37 | DOI: 10.17221/110/2023-CJAS
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Beef, lamb, pork and poultry meat commodity prices: Historical fluctuations and synchronisation with a focus on recent global crisesOriginal PaperMassimiliano CalviaAgric. Econ. - Czech, 2024, 70(1):24-33 | DOI: 10.17221/361/2023-AGRICECON
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Genetic diversity and phylogenetic analysis of Robinia pseudoacacia L. populations using ISSR markers, ITS1 and trnL-F intergenic spacer sequencesOriginal PaperMehmet Emin Uras, Ertugrul Filiz, Ugur Sen, Ibrahim Ilker OzyigitJ. For. Sci., 2024, 70(1):1-13 | DOI: 10.17221/95/2023-JFS Robinia pseudoacacia L. is a deciduous tree planted almost all around the world for a wide variety of uses such as ornamental in urban ecosystems and forest trees in afforestation. This study aims to evaluate the genetic diversity and phylogenetic relations of R. pseudoacacia using some selected populations in Istanbul and Kocaeli cities. For this aim, molecular marker-assisted and DNA sequence-based analyses were performed. According to the results, nine of 15 inter simple sequence repeats (ISSR) primers gave clear and distinguishable bands with a total of 100 loci. The percentage of polymorphic loci (PPL) was calculated as 100% for multi-populations and ranged from 46% to 76% for single populations. Nei's gene diversity value was calculated between 0.165 and 0.251. The lowest and highest PPL were found in populations of Barbaros Boulevard and Dilovası District, respectively. Population structure analysis showed seven different genetic structures for five populations. Internal transcribed spacer 1 region (ITS1) and trnL-F intergenic spacer region were used to examine the phylogenetic relationships of R. pseudoacacia, and both regions showed a high discriminative power at the family level. Based on the findings, R. pseudoacacia, as a forest tree residing in the urban ecosystem, may face the risk of population decline in the upcoming years due to its moderate/low genetic diversity and susceptibility to environmental pressures. |
Comparison of the growth of the provenances of silver fir (Abies alba Mill.) on research plots in the regions Novohradské hory and Èeskomoravská vrchovina at the age of 50 yearsOriginal PaperJiøí Èáp, Martin Fulín, Franti¹ek Beran, Jaroslav Dostál, Martina KomárkováJ. For. Sci., 2024, 70(1):14-23 | DOI: 10.17221/113/2023-JFS The study evaluates the results of a long-term research trial lasting 50 years in two research plots established in the 1970s aimed at solving the decline of the silver fir in the Czech Republic. A total of 103 different provenances occurring naturally across 12 countries were planted in research plots No. 67, Èernovice (Èeskomoravská vrchovina), and No. 77, Nové Hrady (Novohradské hory). The total tree height, diameter at breast height (DBH) and assessment of the health status of all trees in the area were measured with the same methodology. Subsequently, the volume of large wood was calculated by the volume equation, and the growing stock per hectare was recalculated. Based on our analysis of average height growth, it was found that trees with the best growth came from provenances that included the Czech provenances CZ 70 – ®dírec nad Doubravou, CZ 82 – Vizovice, CZ 42 – Lukov, CZ 76 – Nýrsko, Suchý Kámen, and CZ 53 – Opoèno. The last three mentioned provenances also dominated in other growth parameters (DBH and volume of large wood). Of the foreign provenances, D 146 – Schwarzwald mit Baar, S 9 – Kriváò, and S 6 – Èierny Váh produced trees that showed above-average values. The smallest growth in height was found in the trees from the provenances BG 132 – Rila, Borovets, F 104 – Departement de µ Aude, I 228 – Vallombrosa, A 93 – Wörschachwald. Although some trees of Italian provenance achieve above-average volume, due to high mortality, they are not suitable for economic use in the forests of the Czech Republic. Based on the European zoning for forests, the units from the Central European region (3.11.0 – Èeské Polabí, 3.32.0 – Schwarzwald with foothills and Baar, and 9.14.0 – Southern Apennine Mountains) were the best rated in all parameters. Specimens from 9.12.0 – Mountain forest of the northern Apennines, and 4.05.0 – Vosges showed the lowest parameters. |
Characteristics of powdery mildew [Sawadaea bicornis (Wallr.) Miyabe] influence on the photosynthetic process in Norway maple (Acer platanoides L.) seedlingsShort CommunicationAnna Alexeyeva, Kyrylo Holoborodko, Iryna Ivanko, Olexandr Zhukov, Iryna LozaJ. For. Sci., 2024, 70(1):31-39 | DOI: 10.17221/30/2023-JFS The article presents the results of research on the impact of Sawadaea bicornis (Wallr.) Miyabe on the state of photosynthetic apparatus in Acer platanoides L. seedlings using a technique of chlorophyll fluorescence induction (ChlF) measurement, which at the present time can be implemented through the use of biosensors. The research was conducted in September 2022 in the territory of the Botanical Garden of Oles Honchar Dnipro National University. To diagnose a violation of the native chlorophyll photosynthesis in fresh leaves of A. platanoides, a portable fluorometer 'Floratest' was used (the selected spectral range for fluorescence intensity measurement was 670–800 nm). The research was carried out on fresh leaves of Norway maple seedlings both not unaffected and affected with powdery mildew. Analysis of the data obtained indicates a high sensitivity of the parameters of chlorophyll fluorescence induction to damage by the disease regardless of environmental conditions of local growth of A. platanoides seedlings. The high informativeness of induction changes in chlorophyll fluorescence in the structural organisation of chloroplasts in Norway maple leaves determined by the parameters Fo, Fm, Fv, Fv/Fо, Fv/Fm, (Fm – Fst)/Fst, (Fp – Fо)/Fv was revealed. This study demonstrated the effectiveness of using the studied chlorophyll fluorescence parameters to detect severe stress in Norway maple seedlings caused by powdery mildew exposure when the fungus affects more than 50% of the leaf blade area. It is further necessary to conduct dynamic studies throughout the growing season to determine the effectiveness of using these parameters to detect mild stress in the early stages of infection. |
Effect of dietary housefly maggot extract on egg production and egg quality in laying hens under a digital livestock systemOriginal PaperVictor A. Zammit, Sang O. ParkCzech J. Anim. Sci., 2024, 69(2):59-67 | DOI: 10.17221/146/2023-CJAS Antibiotics in poultry feed are banned in many countries owing to their side effects, and insects containing antimicrobial peptides are proven to have potential as antibiotic alternatives in such feed. Thus, the primary objective of this study was to investigate the effects of dietary housefly maggot extract (HME) as the biofunctional material for antibiotic replacement in laying hens. The biofunctional properties of HME on egg production, egg quality, blood biomarkers of immune function, and microbiota were evaluated over a period of 40 to 50 weeks in laying hens under a digital livestock system. A total of 120 forty-week-old Hyline Brown laying hens were randomly divided into four groups with thirty replicates as follows: no added HME (CON), positive control (PC, 8 ppm of avilamycin), 100 ppm of HME (HME100), and 150 ppm of HME (HME150). Egg production and egg weight increased significantly in the HME150, HME100, PC, and CON groups (P < 0.05), but there were no differences between the HME and PC groups. Egg quality, such as eggshell thickness, eggshell strength, Haugh unit, and albumin height, were significantly improved in the HME and PC groups than in the CON group (P < 0.05). A blood biomarker of immune function, IgG, was significantly higher in the HME and PC groups than the CON group (P < 0.05), but there were no differences between the HME and PC groups. Blood corticosterone and heterophil to lymphocyte ratio were significantly lower in the HME and PC groups than in the CON group (P < 0.05). Microbiota Lactobacillus in the faeces were significantly higher in the HME and PC groups than in the CON group (P < 0.05). The faecal total aerobic bacteria, Escherichia coli, and coliform counts were significantly lower in the HME and PC groups than in the CON group (P < 0.05). Consequently, the HME showed the same significant effects as antibiotics on improving egg production and egg quality in laying hens. These results show that 100 ppm of HME can be used as a biofunctional material for an effective alternative to antibiotics in laying hens under a digital livestock system to improve egg production and quality by stimulating their immune functions and balancing the microbiota populations. |
The relationship of soil sulfur with glomalin-related soil protein and humic substances under different mineral and organic fertilisationOriginal PaperPavel Suran, Jiøí Balík, Martin Kulhánek, Ondøej Sedláø, Jindøich ÈernýPlant Soil Environ., 2024, 70(2):93-100 | DOI: 10.17221/493/2023-PSE In recent years, sulfur inputs into the soil have greatly diminished due to the significant decrease in SO2 emissions. Plant nutrients, like sulfur, can be released by the mineralisation of soil organic matter (SOM), which is a complicated mixture of substances (or fractions) like glomalin-related soil protein (GRSP) and fulvic acids (FA), humic acids (HA), humic substances (HS) and others. GRSP, FA, HA, and HS content, as well as the content of mineral and organic fractions of sulfur, was determined in different mineral and organic fertiliser treatments of the long-term field experiment. Using these results, the sulfur content in GRSP was calculated based on the soil’s organic matter carbon and soil’s organic bound sulfur (CSOM/SORG) ratio. Sulfur content in GRSP was 4.08–5.46 (easily extractable GRSP), 9.77–15.7 (difficultly extractable GRSP), and 13.9–21.1 (total GRSP) mg S/kg of soil. Overall, the application of the organic fertiliser caused an increase in S content bound to GRSP. A strong significant relationship was also observed between GRSP fractions and soil organic sulfur. A similar relationship was also observed for the HA and HS with organic sulfur. |
Digital soil mapping using machine learning-based methods to predict soil organic carbon in two different districts in the Czech RepublicOriginal PaperShahin Nozari, Mohammad Reza Pahlavan-Rad, Colby Brungard, Brandon Heung, Lubo¹ BorùvkaSoil & Water Res., 2024, 19(1):32-49 | DOI: 10.17221/119/2023-SWR Soil organic carbon (SOC) is an important soil characteristic as well as a way how to mitigate climate change. Information on its content and spatial distribution is thus crucial. Digital soil mapping (DSM) is a suitable way to evaluate spatial distribution of soil properties thanks to its ability to obtain accurate information about soil. This research aims to apply machine learning algorithms using various environmental covariates to generate digital SOC maps for mineral topsoils in the Liberec and Doma¾lice districts, located in the Czech Republic. The soil class, land cover, and geology maps as well as terrain covariates extracted from the digital elevation model and remote sensing data were used as covariates in modelling. The spatial distribution of SOC was predicted based on its relationships with covariates using random forest (RF), cubist, and quantile random forest (QRF) models. Results of the RF model showed that land cover (vegetation) and elevation were the most important environmental variables in the SOC prediction in both districts. The RF had better efficiency and accuracy than the cubist and QRF to predict SOC in both districts. The greatest R2 value (0.63) was observed in the Doma¾lice district using the RF model. However, cubist and QRF showed appropriate performance in both districts, too. |
Erosion risk analysis in a changing climateOriginal PaperJan Prachowski, Jan Szturc, Josef Kuèera, Jana PodhrázskáSoil & Water Res., 2024, 19(1):50-63 | DOI: 10.17221/110/2023-SWR Soil is an irreplaceable natural resource, with irreplaceable ecosystem functions. One of the greatest risks of soil degradation in the Czech Republic is accelerated erosion, which causes numerous damages to soil properties with negative impacts on the environment. The climate development in recent decades and its forecasts may further intensify these processes. This article deals with the analysis of the impacts of changes in selected climatic factors on the development of erosion processes, which in the conditions of the Czech Republic are influenced mainly by the development of precipitation in the growing season and the development of the values of erosion potential of water released by snowmelt in the winter (non-growing) period. The analysis was carried out on a total area of 459.5 km2, in different morphological and climatic conditions. The impact of climate change was assessed using historical and updated values of rain erosivity and snow erosion potential factors. The results show an increase in the risk of erosive loss in the growing season for all the analysed areas, while the values of erosive loss in the non-growing period differ from each other depending on the climatic and morphological conditions of the areas under study. |
Antifungal effect of fresh and stored olive mill wastewater and its ethyl acetate extract against plant pathogenic fungiOriginal PaperRaja Jarboui, Mona Saber Azab, Hallouma Bilel, Shaima M.N. MoustafaPlant Protect. Sci., 2024, 60(1):65-79 | DOI: 10.17221/91/2023-PPS Olive mill wastewater (OMW) has serious environmental implications due to its high organic matter content, particularly its phenolic compounds. Using OMW in crop protection has been suggested as an environmentally friendly alternative to reduce the impact of chemical pesticides on human health and the environment. This study aimed to investigate the antifungal activity of fresh and stored OMW, as well as its ethyl acetate extract, against several phytopathogenic fungi: Syncephalastrum racemosum, Paramyrothecium roridum, Fusarium oxysporum, and Verticillium dahliae. OMW was stored at 25 °C and 45 °C for three months, and both fresh and stored OMW were used in non-sterile, sterile, and centrifuged conditions. Phenolic and flavonoid compounds were extracted and identified by high-performance liquid chromatography (HPLC) analysis. Results showed that fresh OMW and its derivative compounds significantly inhibited the studied fungi. In contrast, OMW storage, sterilization, and centrifugation increased the mycelium growth of the fungi, particularly S. racemosum, which demonstrated relative resistance to stored OMW and its ethyl acetate extract. During storage, some phenolic and flavonoid compounds disappeared (resorcinol and vanillic acid), while the concentration of others increased (gallic acid, chlorogenic acid, and quercetin). This work highlights the potential use of fresh OMW as a bio-agent to protect plants from fungal diseases. |
Sex ratio dynamic of the field population of the sugarcane rust mite Abacarus sacchari (Acari: Eriophyidae)Original PaperNur Asbani, Hardev Sandhu, Oscar Liburd, Julien Beuzelin, Ronald Cherry, Gregg NuesslyPlant Protect. Sci., 2024, 60(1):89-96 | DOI: 10.17221/50/2023-PPS Sugarcane in southern Florida suffers from sugarcane rust mite (SRM) infestations, Abacarus sacchari, from summer to early fall. The mite’s sex ratio is important in understanding the mite's demography and population dynamics. This is the first report on the sex ratio of the field population of the SRM. The objectives of the study were to determine the sex ratio of the SRM and the factors that affect any changes in the sex ratio. To determine the sex ratio, mites were sampled from a sugarcane canopy each month over a 12-month period and the sex ratio was expressed as the proportion of females to the total number of sexed mites. The population density and aerial mites were monitored weekly for 8 and 6 months, respectively. The total number of sexed mites from the sugarcane canopy was 27 941 mites, while 2 248 airborne mites were recorded. The result showed that the sex ratio of the SRM in the canopy was dynamic during the study, with a female bias more common than a male bias among the samples, which ultimately resulted in a slightly female bias. An obvious change from a female bias to a male bias occurred simultaneously with the increased mite density and dispersal. The factors affecting the oscillation of the sex ratio are discussed. |
Subsidy policies for the grain supply chain considering postharvest loss of grain and agricultural pollutant emission in ChinaOriginal PaperPan Liu, Bin Zhao, Haodong Tang, Jiamin ZhuAgric. Econ. - Czech, 2024, 70(5):207-225
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Increasing Boro rice productivity through credit: Evidence from BangladeshOriginal PaperShah Johir Rayhan, Md. Sadique Rahman, Kaiyu LyuAgric. Econ. - Czech, 2024, 70(2):49-59 | DOI: 10.17221/341/2023-AGRICECON
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Valuation of an innovative investment project using real options approach: A case study of a viticulture company in SpainCase StudyRaisa Pérez-Vas, Javier Hervés-Estévez, María Dolores Garza Gil, Raquel Fernández-GonzálezAgric. Econ. - Czech, 2024, 70(2):91-100 | DOI: 10.17221/299/2023-AGRICECON
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Pharmacokinetics of praziquantel and its metabolites in grass carp (Ctenopharyngodon idella) following the oral administration of a single bolusOriginal PaperR Dobsikova, J Blahova, P Marsalek, V Doubkova, E Zuskova, J VelisekVet Med - Czech, 2024, 69(2):52-60 | DOI: 10.17221/109/2023-VETMED The study aimed to evaluate and compare the routes and rates of the depletion of the antiparasitic praziquantel (PZQ), a derivative of pyrazinoisoquinoline, following its oral administration in grass carp (Ctenopharyngodon idella). We focused on the depletion of PZQ and its major metabolites – cis-hydroxy praziquantel (CPZQ) and trans-hydroxy praziquantel (TPZQ), in water, the plasma, hepatopancreas, kidney, muscle, and skin, following a single oral administration of PZQ in a concentration of 50 mg/kg. Fish were sampled before the drug administration and then eight times in the course of the 30-day-long experiment. Our results indicate the rapid absorption and elimination of PZQ and its metabolites in all the analysed matrices. The most PZQ-burdened tissue was the hepatopancreas, the gill and the skin. In all the samples, the concentration of the drug and its metabolites consistently declined over time. The residue of the parent compound was detected for the longest time in all the tissues. During the study, a significant (P < 0.01) correlation was found within the concentration of PZQ, CPZQ, and TPZQ in the water and all the biological matrices. It was also found that the PZQ residue was not detected below the maximum residue levels (i.e., 20 µg/kg) until 16 days after exposure in the muscle and skin. |
Effect of compounds used for soaking narcissus bulbs on mycelium coverage of bulbs and substrateOriginal PaperAdam Tadeusz Wojdy³a, Jacek Stanis³aw Nowak, Jan Bocianowski, Monika Ka³u¿na, Jacek Wi¶niewski, Emilia WaszkiewiczHort. Sci. (Prague), 2025, 52(2):103-110 | DOI: 10.17221/42/2024-HORTSCI In the experiments, two compounds were used: hydrogen peroxide with silver (H2O2-Ag+) and captan, pyraclostrobin + boscalid and methyl thiophanate + tetraconazole to soak narcissus bulbs before planting for the period of 20 minutes. The bulbs were planted in such a way that 2/3 of the bulb was above the surface of the substrate. All the pots with bulbs were subsequently placed in plastic boxes and transferred to a cooling chamber adjusted precisely for 9 °C and 94–99% air humidity. During the rooting of the bulbs in the cold room after 4, 8, 12 and 15 weeks, and then in the greenhouse after cutting the above-ground parts, the observations of the bulbs and percentage substrate coverage by the fungi most frequently isolated in the previous observations. The research showed that H2O2-Ag+ and fungicides used for soaking significantly limited the fungal development on bulbs and also on the substrate during the rooting period in the cold store and after they were placed in the greenhouse until the flowering. |
Enhancing cattle production and management through convolutional neural networks. A reviewReviewJean de Dieu Marcel Ufitikirezi, Roman Bumbálek, Tomá¹ Zoubek, Petr Barto¹, Zbynìk Havelka, Jan Kresan, Radim Stehlík, Radim Kune¹, Pavel Ol¹an, Miroslav Strob, Sandra Nicole Umurungi, Pavel Èerný, Marek Otáhal, Lubo¹ SmutnýCzech J. Anim. Sci., 2024, 69(3):75-88 | DOI: 10.17221/124/2023-CJAS The rise in demand for animal products associated with global population growth has driven the world toward precision livestock farming, where convolutional neural networks (CNN) have gained increasing attention due to their potential to enhance animal health, productivity, and welfare. However, the effectiveness and generalizability of CNN applications in cattle production are limited by several challenges and limitations, which require further research and development to address. This systematic literature review aims to provide a comprehensive overview of the applications of CNN in cattle production. It identified some potential applications of CNN in this field and highlighted the challenges and limitations that need to be addressed to improve the effectiveness and efficiency of CNN applications in cattle production. It also provides valuable insights for researchers, practitioners, and policymakers interested in the use of CNN to enhance cattle production practices, animal welfare, and sustainability. Additionally, it also provides the reader with a summary of the literature on the fundamental concepts of convolutional neural networks and their commonly used model architectures in cattle production. This is because agriculture digitalisation is going more multidisciplinary and people from different areas of expertise may find it helpful to learn more from a combined source. |
Fabrication and performance test of a multipurpose ohmic heating apparatus with a real-time data logging system based on low-cost sensorsOriginal PaperDiang Sagita, Dadang Dayat Hidayat, Doddy Andy Darmajana, Ari Rahayuningtyas, Hari HariadiRes. Agr. Eng., 2024, 70(1):23-34 | DOI: 10.17221/21/2023-RAE Ohmic heating is an emerging technology currently in high demand for application in various processes. In this research, a prototype of a multipurpose ohmic heating apparatus was successfully designed, fabricated, and tested. This apparatus was designed based on low-cost and versatile sensors and components available worldwide. Three independent chambers could be operated parallelly and individually with different treatments. Parameter data, i.e., voltage, electrical current, the temperature of heated material and environmental humidity-temperature, could be recorded by an embedded data logging system. The sensor had been tested and validated by comparing all the sensors used with commercial standard instruments. The result showed that all sensors had high measurement accuracy, indicated by very low mean absolute error (MAE) and mean absolute percentage error (MAPE), with R2 > 0.999. The performance test revealed that product temperature could be accurately maintained according to the set point temperature with a deviation value lower than 0.1 °C. The data logging system was able to record and store the parameter data in SD card memory for up to several days without interruption. The prototype of the ohmic heating apparatus could be an effective alternative to process many purposes such as pasteurisation, cooking, warming, and fermentation based on the ohmic heating principle. |
Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosaOriginal PaperB Lipovy, L Vacek, D Polastik Kleknerova, E Jeklova, L Liskova, J Holoubek, D Matyskova, F RuzickaVet Med - Czech, 2024, 69(6):191-197 | DOI: 10.17221/25/2024-VETMED Pseudomonas aeruginosa poses a significant threat to both immunocompetent and immunocompromised individuals, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for P. aeruginosa complicated skin and soft tissue infections (cSSTIs). This study presents a novel porcine model inducing and sustaining cSSTI for 14 days. Six pigs (120 wounds) were used for the development of infections, and within this group, two pigs (40 wounds) were used to evaluate the progression of the cSSTI infection. The model demonstrated bacterial loads of more than 107 CFU/gram of tissue or higher. The cSSTI fully developed within three days and remained well above these levels until day 14 post-infection. Due to the immunocompetence of this model, all the immunological processes associated with the response to the presence of infection and the wound healing process are preserved. |
Location-specific technology transfer model in an agricultural technology park, IndonesiaOriginal PaperHarmi Andrianyta, Elisa Anggraeni, Sapta Raharja, Sukardi SukardiRes. Agr. Eng., 2024, 70(2):61-72 | DOI: 10.17221/7/2023-RAE Diversity in the characteristics of agricultural locations has the potential to develop. Unfortunately, the weak transfer of technology based on the characteristics of the location indicates that this potential cannot grow properly. This research aims to synthesise a technology transfer model for an agricultural technology park (ATP) by considering site-specific conditions. This cross-case study was conducted at selected ATP locations. Model synthesis uses the system development life cycle stages of initiation, analysis, and design. The resource-based view approach was broken down into several variables during the analysis stage. Three location-specific technology transfer models were successfully developed: a technology transfer model based on highland specificity, a unique technology transfer model for urban farming, and a tourism village-based model. |
Transitional, fused and/or supernumerary vertebrae in the lumbosacrocaudal region of the spine – A reality in many domestic mammalsOriginal PaperC Toader CovasaVet Med - Czech, 2024, 69(5):156-168 | DOI: 10.17221/102/2023-VETMED The aim of this study was to identify the presence of transitional lumbosacral and sacrocaudal vertebrae in domestic mammals, to realise a comparative analysis of the localisation and conformation of this abnormal condition. The research included the following species: cattle – 29 specimens, sheep – 32 specimens, horse – 31 specimens, pig – 26 specimens, rabbit – 33 specimens, dog – 89 specimens and cat – 57 specimens. The spine of the animals was analysed post-mortem or radiologically. The investigations revealed the presence of transitional vertebrae as follows: in cattle – 3 cases (8.7%), all being about the lumbarisation of the first sacral vertebra (S1); in sheep – 3 cases (9.37%), two lumbarisation cases of S1 and one caudalisation of S4 (the last sacral vertebra); in horses – 4 cases (12.9%), all about the sacralisation of Cd1 (first caudal vertebra); in pigs – 3 cases (11.53%), two lumbarisation cases of S1 and one sacralisation of Cd1; in rabbits – 3 cases (9.09%), a lumbar supernumerary vertebra (L8) and two cases of caudalisation of S4; in dogs – 4 cases (4.49%), a lumbar supernumerary vertebra (L8) and 3 cases of sacralisation of the last lumbar vertebra (L7 or L8); in cats – 3 cases (5.26%), two sacralisation cases of the last lumbar vertebra and one case of caudalisation of the last sacral vertebra (S3). A strong lumbarisation process was observed in ruminants (especially in cattle), then in pigs, the sacralisation being prevalent in carnivores. The sacrocaudal transitional vertebra was predominant in horses. No evident influence of the sex and age of the animals was observed. |
Assessment of agricultural carbon emissions reduction potential and optimisation pathways based on a framework of equity and efficiency principles: Evidence from Fujian Province in ChinaOriginal PaperJie Ye, Renshan Xie, Xingwei Deng, Minling Lin, Yang Chen, Ketao Lin, Jianzhou YangAgric. Econ. - Czech, 2024, 70(3):125-136 | DOI: 10.17221/329/2023-AGRICECON Fujian Province, China was chosen as the study area for estimating the marginal agricultural abatement costs within the province based on data for 2010–2020. The study employed these estimations as a pivotal factor affecting the efficiency of carbon emissions reduction, constructed an index model to gauge the potential of agricultural carbon emissions reduction, and delved into the urban agricultural carbon emissions reduction potential from the perspectives of economic development rights (equity) and carbon emissions reduction difficulty (efficiency). Our findings indicated a marked regional disparity in the marginal abatement costs of agriculture across Fujian Province, with the highest recorded at EUR 1.3771 × 108 per 104 tonnes and the lowest at EUR 0.6526 × 108 per 104 tonnes, albeit demonstrating general upward inter-annual trends. Furthermore, the assessment of agricultural carbon emissions reduction potential, grounded in the principles of equity and efficiency, revealed four distinct developmental tiers. Resource allocation pathways for carbon emissions reduction were subsequently delineated, informed by the stratification of high- and low-carbon emissions reduction potential indices alongside typological characteristics. The outcomes of this study offer strategic guidance to the government of Fujian Province in crafting suitable carbon emissions reduction strategies and in devising targeted action plans aimed at achieving the twin goals of 'carbon peaking' and 'carbon neutrality'. |
