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The aim of this study was to monitor the Czech Aberdeen Angus population for backfat thickness (BT), musculus longissimus lumborum et thoracis (MUSCLE) and live weight (LW) in relation to environmental and genetic conditions. In this study, we also wanted to display the potential for future inclusion of these parameters into national monitoring. Parameters of BT, MUSCLE and LW were measured at 120, 210, and 365 days of age in 769 Aberdeen Angus calves (417 bulls and 352 heifers) on seven farms. Statistical evaluation was performed in the SAS v9.3 software. The bulls achieved significantly higher (P < 0.01) weights and larger MUSCLE compared to the heifers, although the heifers had higher BT. The age of the dam at calving significantly influenced the growth ability of tested calves, but it did not influence BT nor MUSCLE. The nested effect of the year-season, farm and sire bulls (or genetic background) significantly affected LW, BT and MUSCLE of tested calves. The use of the same breeding bulls on different farms resulted in significant differences in growth parameters. Our results displayed the potential of nationwide monitoring of MUSCLE and BT of beef breeds, as official parameters of the beef performance testing in the Czech Republic. The inclusion of these globally used traits in the Czech breeding system would allow to predict breeding values for the Czech beef population. This would lead not only to general improvement of MUSCLE in the population, but also to the production of better breeding animals and slaughter animals with higher value.

The objective of this study was to evaluate the inter-breed genetic diversity of Lithuanian Trakehner horses. The current population in Lithuania numbers 1 039 Trakehner horses. The study included the immunogenetic data analysis of 316 blood samples. Pedigree was traced back to 7-11 generations. The horses were assigned to genealogical clusters originating from East Prussian Trakehner Stud, Thoroughbred and Arab sires. The first two clusters were subdivided into nine-horse groups according to the most important ancestors. The evaluation of genetic diversity was based on six blood groups and five protein polymorphism systems. The differences between genealogical groups were confirmed by principal component analysis and applied cluster analysis. According to the allelic frequency, the Trakehner population changed very slightly through a 30-year period, and only one rare (0.013) allele Tf^RR (frequent in other Trakehner populations), has been newly found. The analysis of genetic polymorphism systems based on a very low rate of alleles Q^abc (0.093) and D^dkl (0.046), which are typical of other populations, indicated the exclusivity of the Trakehner population in Lithuania. The average expected heterozygosity by blood protein polymorphism and blood groups were 0.409 and 0.441, respectively. The genetic clustering diverged by observed heterozygosity of blood groups and by pedigree data in Pilger through Egoist, Bay Ronald through Dark Ronald and Dampfross through Hyperion subgroups. The determined distinctiveness of subdivided Trakehner horse groups suggests reconsidering the breeding strategies and conservation programme of Trakehner horses. Breeding and selection of subdivided sire lines could be among the appropriate solutions for the maintenance and extension of the genealogical structure.

The paper shows a large-scale shift in agroclimatic zones in the territory of the Czech Republic (CR) between 1961 and 2019. The method used for agroclimatic zoning took advantage of high-resolution (0.5 km × 0.5 km) daily climate data collected from 268 climatological and 787 rain-gauge stations. The climate information was combined with soil and terrain data at the same resolution. The set of seven agroclimatic indicators allowed us to estimate rates of changes in agroclimatic conditions over the 1961-2019 period, including changes in the air temperature regime, global radiation, drought, frost risks and snow cover occurrence. These indicators are relevant for all main crops and agroclimatic zoning and account for local soil and slope conditions. The study clearly highlights major shifts in the type and extent of agroclimatic zones between 1961-2000 and 2000-2019, which led to the occurrence of entirely new combinations of agroclimatic indicators.

Slow-release nitrogen fertiliser can potentially increase crop production and improve fertiliser nitrogen use efficiency. However, it is unclear that are suitable for different regions and crops in the northeast of China. Therefore, according to different soil and climate characteristics, we investigated the synchronised relationships between nitrogen slow release fertiliser and nitrogen maize requirements. Experiments were conducted at Shenyang Agricultural University, Liaoning province, Northeast China, from 2016 to 2017. Stabilised fertiliser treatments increased grain yield, nitrogen use efficiency and nitrogen accumulation at each maize growth stage. Grain yield increased by 2.32% and 11.33% (2016), and 1.55% and 7.87% (2017), respectively, when compared with the urea CK1 (233 kg N/ha) and CK2 (210 kg N/ha) treatments. Additionally, during the growth period of the stabilised fertiliser treatment, the stability of the synchronisation relationship between nitrogen absorption and absorption of spring maize was significantly higher than other treatments, and the effect was the best. Therefore, we conclude that the stabilised fertiliser is the most suitable option for promotion and application in spring maize in Northeast China.

Although various studies of codon usage bias have been reported in a broad spectrum of organisms, no studies to date have examined codon usage bias for herbicide target genes. In this study, we analysed codon usage patterns for the acetolactate synthase (ALS) gene in eight monocot weeds and one model monocot. The base composition at the third codon position follows C3 > G3 > T3 > A3. The values of the effective number of codons (ENC or Nc) indicate low bias, and ENC or Nc vs. GC3 plot suggests that this low bias is due to mutational pressure. Low codon adaptation index and codon bias index values further supported the phenomenon of low bias. Additionally, the optimal codons, along with over- and under-represented codons, were identified. Gene design using optimal codons rather than overall abundant codons produce improved protein expression results. Our results can be used for further studies, including eliciting the mechanisms of herbicide resistance (occurring due to elevation of gene expression levels) and the development of new compounds, their efficiency and risk assessment for herbicide resistance evolution.

Soil incubation and pot experiments were conducted to follow the sorption processes of added phosphorus (P) fertiliser using the radioisotope tracer technique. Increasing doses of P fertiliser (40, 80, 160, 320 mg P/kg soil) were added to Chernozem and Arenosol and incubated for 1, 3, and 13 weeks. After incubation, perennial ryegrass (Lolium perenne L.) was sown in one group of pots, and the experiment had been continuing for another 9 weeks. The yield, grass P uptake, isotopically exchangeable (P_IE), water-soluble (P_W), and ammonium lactate soluble phosphorus (P_AL) fractions of soils were measured. On Chernozem, plant P uptake, P_IE, P_W and P_AL were significantly less in the case of the longest incubation period compared to shorter incubations. This suggests a transformation of P into tightly sorbed form. On Arenosol, there were only small changes in the parameters as the incubation period increased, suggesting less intense P transformation to tightly sorbed form. The P_W/P_IE ratio enhanced with increasing P-doses, and the ratios were higher on Arenosol. On Arenosol, the higher P doses caused a greater increase of P_W than on Chernozem. The P_IE + P_W showed a good correlation with plant P uptake proving this value can be a good indicator of plant-available phosphorus.

This research optimised the application of a hexane-methanol mixture as a binary solvent for the concurrent oil-resin extraction and separation from Calophyllum seeds on a pilot scale, in a direct stage. The optimum oil and resin yields were determined by optimising the extraction conditions using response surface methodology and a second order polynomial model. The extraction conditions affected the oil and resin yields, with the extraction time as the biggest influencing factor. Optimum oil (65%) and resin (16%) yields were predicted to be obtained at 5.2 h and 433 rpm. The model validation with these extraction conditions showed that the predicted results and actual oil (62%) and resin (15%) yields were in passable agreement. The oil was composed of 75.4% triglycerides with a density of 0.874 g.cm^-3, a viscosity of 26.4 mPa.s^-1, an acid value of 46.4 mg KOH.g^-1, an iodine value of 98.0 g iodine.100 g^-1, trace water and sediment contents, and zero ash content. The resin had a viscosity of 4 694.8 mPa.s^-1, a total phenolic content of a 4.51% gallic acid equivalent, an antioxidant activity of an 8.82 mg ascorbic acid equivalent.g^-1, and an acid value of 126.2 mg KOH.g^-1.

Soils that contain rock fragments (particles > 2 mm in diameter) are distributed all over the world. The presence of these small rock fragments can have a great impact on soil water retention properties, as well as on the soil-water infiltration and vegetation restoration in semi-arid regions. To quantitatively describe the transport of water in stony soils, repacked soil cores were used to determine the infiltration rates for different rock fragment contents (0%, 10%, 20%, 30%, and 40%) and rock fragment sizes (2-5, 5-8, 8-11, and 2-11 mm). The results showed that both the content and size of the rock fragments and their interaction significantly affected the infiltration process. The infiltration rates over time and the saturated hydraulic conductivity (K_s) decreased with an increasing rock fragment content to an observed minimum value for a 40% rock fragment content. The soil-water infiltration processes were accurately described by the Kostiakov model. The measured and calculated K_s values decreased with an increasing rock fragment content, which was in accordance with the published data and in accordance with the K_s obtained by five empirical methods. The variations in the measured K_s were likely due to the variations in the soil properties caused by the soil sample repacking. The results of this study may improve the understanding of the effects of the rock fragment content and size on the infiltration processes in arid and semi-arid desert steppes.

External skeletal fixation has been used for the management of fractures of many types of bone. The use of polymeric free-form connecting bars in external fixators has become widely used in veterinary orthopaedics because of its versatile forms of frame construction and its relative low costs. Determining thermal-transfer to trans fixation pins during polymerisation of poly(methyl methacrylate) and epoxy putty polymers used for free-form external skeletal fixation connecting bars is important for avoiding temperatures of more than 47 °C, which would cause thermal soft tissue injury. Therefore, thermal transfer to trans fixation pins was measured in vitro during the polymerisation phase of these polymers. We used trocar-pointed pins of different diameters that punctured one wall of a connecting tube, resulting in the tip of the trocar-pointed pin reaching the centre of the tube. The FLUKE^® VT02 infrared digital camera was then used to measure heat transfer to the pins at 1 or 2 cm from poly(methyl methacrylate) or epoxy putty. The polymerisation temperatures of these polymers yield a potentially dangerous level of heat for soft and hard tissue. This was observed in almost all the experimental conditions tested. On the other hand, epoxy putty transfer to the pins did not cause the temperature to reach 47 °C at any time or in any of the setups examined. Poly(methyl methacrylate) did reach more than 47 °C and remained at that level for more than 1 min at 1 cm from the polymer. This acrylate exhibited polymerisation temperatures higher than epoxy and its heat transfer to the pins was potentially dangerous if used at less than 1 cm from soft or hard tissue.

The aim of this study is to evaluate the outcome of the treatment on twenty wild birds presenting with fractures that were stabilised with modified Meynard external fixators. The study material consisted of a total of twenty birds of eight different species, six white storks (Ciconia ciconia), one eastern imperial eagle (Aquila heliaca), one European honey buzzard (Pernis apivorus), one long-legged buzzard (Buteo rufinus), three common buzzard (Buteo buteo), one northern goshawk (Accipiter gentilis), one Armenian gull (Larus armenicus), and six yellow-legged gulls (Larus michahellis), which were diagnosed with fractures in their extremity bones following clinical and radiological examinations. The lesions of the twenty cases evaluated in the study were diagnosed as humerus fractures in nine cases, ulna fractures in three cases, tibiotarsus fractures in seven cases and a femur fracture in one case. The external fixators were removed between weeks five and nine postoperatively. While healing was seen in twelve of the twenty evaluated cases, the complete functional recovery occurred in six of these and they were released into nature. It was concluded that modified Meynard external fixator, which was made applicable for fractures in birds, could be an alternative method for fracture treatments in wild birds.

The objective of this prospective randomised clinical study was to determine the differences in the tracheal, oesophageal and rectal temperature in spontaneously breathing or mechanically ventilated dogs. A total of thirty dogs were allocated to the SPO-group breathing spontaneously (n = 15) or the MEC-group ventilated mechanically (n = 15). Anaesthesia was established using a medetomidine-butorphanol-propofol-isoflurane combination. The tracheal (T-Tra), oesophageal (T-Oes), rectal (T-Rec), inspired gas (T-Gas), room (T-Room) temperatures, respiratory frequency (f_R), heart rate (HR), mean arterial pressure (MAP) and end-tidal carbon dioxide concentration (ETCO₂) were measured after connecting to a re-breathing system (baseline) and subsequently in 10-minute intervals for 60 minutes. The data were analysed using ANOVA and Steel-Dwass tests (P < 0.05). In the SPO-group, the T-Tra, was significantly lower at T30, T40, T50, T60, the T-Oes and T-Rec at T40, T50, T60, compared to the baseline. In the MEC-group, the T-Tra and T-Oes was significantly lower at T30, T40_, T50, T60, the T-Rec at T40, T50, T60, compared to the baseline. In the SPO-group, the f_R was significantly lower for all the times and the ETCO₂ higher at T10, T20, T30, T40, T50 compared to the MEC-group. No other differences were detected. During anaesthesia, there is a comparable decrease in body temperatures, regardless of whether the dogs are breathing spontaneously or ventilated mechanically.

The standardisation of protocols and discussion of therapeutic procedures in the rehabilitation of turtles affected by oil spills are necessary to optimise the recovery time and increase the chances of survival of these animals. This study aimed at reporting the processes adopted for the stabilisation, decontamination, rehabilitation and release of an oiled olive ridley sea turtle (Lepidochelys olivacea), rescued alive on September 23, 2019, at Santa Rita Beach, Extremoz municipality, Rio Grande do Norte, Brazil. Its entire body was covered by oil. At first, the animal was mechanically dry cleaned using a gauze soaked in mineral oil in the keratinised regions (carapace and plastron) and a gauze soaked in vegetable oil was used on the oral, nasal, ocular, and cloacal mucous membranes. The second stage of the oil removal consisted of washing the animal with heated pressurised water (39 °C) and a neutral detergent using a soft foam sponge. The animal received treatment with antitoxins, antibiotics, analgesics, gastrointestinal protectors, and fluid therapy. After 7 days of treatment, the blood count showed that all the parameters were within the normal range. The oil cleaning process and the therapeutic protocol used in the rehabilitation of the olive ridley sea turtle were efficient.

A congenital defect that blocks the cerebrospinal fluid outflow is the most common cause of hydrocephalus in young cats. It usually manifests itself within a few weeks of birth. Diagnostic imaging is essential. Therefore, it was decided to report on, for the first time, a clinical case of congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy. A difference between the size of the skull bones in the Maine Coon foetuses was recognised during a routine pregnancy ultrasound. Analysis of the sonogram of the bones and brain structures of the diagnosed foetus revealed that the features were highly suggestive of a hydrocephalus. Five clinically healthy kittens and one with hydrocephalus were delivered without complications. The necropsy of the malformed foetus was consistent with the ultrasonographic findings. Bacteriological and virological tests were performed and all were negative which suggest a congenital background of the malformation. This is the first reported case of an intrauterine ultrasound diagnosis of congenital hydrocephalus in a Maine Coon of multiple gestation. The ultrasound control along with the foetometry seem to be useful tools to recognise certain pathologies of pregnancy in cats, including congenital malformations of various origins, which is of significant diagnostic and prognostic importance.

The aim of this retrospective study was to assess the abdominal fat distribution in toy breed dogs using computed tomography (CT) in relation to the breed, age, and sexual status. In 140 dogs (52 Maltese, 33 Poodles, 32 Shih-Tzus, and 23 Yorkshire Terriers), the total fat area (TA), visceral fat area (VA), subcutaneous fat area (SA) and body area (BA) were measured at the third and sixth lumbar vertebral level on non-contrast transverse CT images. The differences in the TA/BA and VA/SA according to the breed, age, and sexual status, and correlations with the age were analysed. The differences in the TA/BA and VA/SA among the breeds were revealed (P < 0.05). There was no difference for the TA/BA among the sexual statuses, but the VA/SA was higher in spayed females than in intact females (P = 0.001). Positive correlation of the age with the TA/BA in the Maltese, Poodles, and intact females, and the age with the VA/SA in the Maltese, Shih-Tzus, Yorkshire Terriers, neutered males, and spayed females were found. The results showed that the abdominal fat composition varied according to the breed, age, and sex, which may have implications on defining obesity-related disease risks in different populations. Careful monitoring of the VA/SA in the breed (Maltese, Shih-Tzu, and Yorkshire Terrier), age (senior dogs), and sexual status (neutered dogs) may be required.

An 8-year-old, neutered male Yorkshire Terrier dog presented with left ventromedial canthus swelling over a one-month period, refractory to pharmacological therapy. There was no history of trauma. On ultrasonography, the lesion had a cystic character filled with anechoic fluid and hyperechoic sedimenting debris. The computed tomography (CT) and CT-dacryocystography showed a cystic lesion protruding from the lacrimal sac fossa and occupying a defect in the orbital plate and an ethmoidal ectoturbinate surrounded by a bony structure with an intact nasolacrimal system. The dog underwent the surgical resection of the cyst and its fluid content was aspirated. Ethmoid mucocele was diagnosed based on the CT, cytologic examination, bacterial culture and histopathologic findings. This case describes the imaging characteristics of an ethmoid mucocele and highlights the importance of CT and CT-dacryocystography in dogs with ventromedial canthus swelling that had poor response to medical treatment.

A ten-year-old indoor, castrated male Cardigan Welsh Corgi (Canis familiaris) presented with the chief complaints of chronic vomiting, retching, hypersalivation, and bilateral submandibular masses for two months. The systemic examinations, including serum chemistry, radiography, ultrasonography, and fluoroscopy, were unremarkable. A fine-needle aspiration revealed bilateral submandibular sialadenitis. Broad-spectrum antibiotics with phenobarbital were prescribed to alleviate the ptyalism. Thereafter, the left submandibular glands were normalised, and the right submandibular glands decreased to half their size. Three weeks later, the animal had an emergency visit because of a sudden left exophthalmos. Computed tomography and magnetic resonance imaging revealed enlarged left zygomatic and right mandibular salivary glands. The affected glands were surgically removed; the histopathologic examination confirmed non-septic sialadenitis, and the patient was finally diagnosed with idiopathic sialadenitis. Vomiting continued after the gland removal and the dog required a gradual increase in the phenobarbital dosage and an additional antiepileptic drug (potassium bromide) to manage the symptoms. The patient died eight months later from an unknown cause. This case report of bilateral submandibular sialadenitis concurrent with unilateral zygomatic sialadenitis in a Welsh Corgi dog suggests that when multiple salivary glands are involved, the response to anti-epileptic drugs and the prognosis is poor compared to that involving a single salivary gland.

The effect of simultaneous intercropping of winter wheat (Triticum aestivum L.) with different legumes of faba bean (Vicia faba L.), pea (Pisum sativum L.), and purple clover (Trifolium incarnatum L.) on selected production and qualitative parameters of wheat was evaluated in field trials conducted both in organic and conventional cropping systems, in comparison with pure sown wheat. Wheat intercropped with legumes achieved higher grain yield compared to pure sown wheat for an average of two years. However, in organic cultivation, the positive effect of intercropping on wheat yields was more pronounced. In addition, a strong influence of the year was noted. In 2021, in the organic cropping system, the most yielding intercropped wheat (especially with pea and bean) achieved 114-117% higher yields compared to pure sown wheat (in the previous year of 2020, it was usually only about 102-106%). In the conventional cropping system, the effect of intercropping on wheat yield was significantly weaker, and in 2021, wheat intercropped with legumes reached even lower yields than pure sown wheat in some cases. In terms of sowing methods (both in organic and conventional cropping systems), mixed sowing with individual legumes significantly exceeded the yields of wheat grown with legumes in separate, alternating rows. As regards quality parameters, wheat intercropped with legumes reached in comparison with pure sown wheat usually had higher crude protein content in wheat grain dry matter and higher values of Zeleny sedimentation.

In this study, a non-linear control system was designed and proposed to control the greenhouse climate conditions. This control system directly uses the information of sensors, installed inside and outside the greenhouse. To design this proposed control system, the principles of a non-linear control system and the concepts of equilibrium points and zero dynamics of system theories were used. To show the capability and applicability of the proposed control system, it was compared with an integral sliding mode controller. A greenhouse with similar climatic conditions was used to simulate the performance of the integral sliding mode controller. In this study, it was seen that the integral sliding mode control system was more accurate; however, the actuator signals sent by this control system were not smooth. It could damage and depreciate the greenhouse equipment more quickly than the proposed non-linear control system. It was also shown that the regulation of the temperature and humidity was performed very smoothly by changing the reference signals according to the weather conditions outside the greenhouse. The ability of these two control systems was graphically demonstrated for temperature and humidity responses as well as for the signals sent to the actuators.

Preferential flow is associated with potential issues of poor irrigation water-fertiliser efficiency in a cultivated field. In addition, a preliminary understanding of how irrigation quotas contribute to this prevalent phenomenon is limited. Thus, one blank control group and three different irrigation quotas were set (0, 450, 550 and 650 mm) and the dye tracing image method was applied to investigate the characteristics of the preferential flow in cotton fields under mulched drip irrigation. On the basis of the results, we found significant differences in the preferential flow degree between the four groups (P = 0.02); the mean scores of the dyed area ratio D_c and variation coefficient C_v from the soil stained profile were 29.83%, 45.77%, 37.36%, 39.40% and 0.98, 1.12, 1.28, 1.17 for the total irrigation quota 0, 450, 550 and 650 mm, respectively, indicating an increasing and then decreasing tendency for the non-uniformity as well as variation in the soil water flowing as the irrigation quota being put on. At the same time, the preferential flow ratios showed a similar trend compared with D_c as well as C_v, which were 4.64%, 13.70%, 40.03%, and 23.60% for the irrigation amounts of 0, 450, 550, and 650 mm, respectively. In general, we concluded that the degree of preferential flow with an irrigation quota of 550 mm (local irrigation practice) was highest while no irrigation led to a more uniform flow in the cotton fields with film mulched. The present study goes some way towards supplementing our understanding of preferential flow in agricultural practice.

The project dealt with an evaluation of the soil quality in the Central Bohemian Region in the Czech Republic. The relevant attributes and characteristics were found regarding the soils in this selected area. Based on the data from soil probes, climate characteristics, soil production function and data on the land use, the characteristics, known as soil quality indicators, were selected. Then the soils were sorted into groups which indicated their suitability for the best land use and planning. The characteristics of the soils that contributed the most to the ecosystem services provided by this part of the environment were chosen as the soil quality indicators. In order to find out how the soils are able to provide ecosystem services, two types of approaches were used - the average score and the total amount of points gained. Maps indicating the soil quality were created using the ArcGIS program. At the same time, research on the differences in the quality in two different layers of the soil was carried out. In most cases, there was a decrease in the soil quality with an increasing depth. The results of this project can be used as a basis for a new soil valuation in the Czech Republic.

With the surrounding environment of Inner Mongolia lead (Pb) ore as the research background, the germination and physio-biochemical effects of Pb stress on alfalfa were discussed to employ this species for the remediation of Pb contaminated soil. Research has shown that a low Pb stress concentration could improve the biological resistance of alfalfa seeds, while a high Pb stress concentration cannot be tolerated. Interestingly, when the Pb concentration was 5 mg/L, the germination rate of the seed was promoted, and the chlorophyll content was especially increased. As the Pb content and stress increased, the amount of malondialdehyde (MDA), H2O2, catalase (CAT) increased; while the root cell viability, chlorophyll and soluble protein content decreased. In consequence, alfalfa was tolerant to Pb stress of 5 mg/L, inversely, its growth was inhibited at levels higher than 5 mg/L, and it was poisoned at 500 mg/L. Based on the principal component analysis (PCA), the H2O2, O2-, chlorophyll total, chlorophyll a, CAT and proline content explicitly reflected the change in the physiology on the alfalfa and its tolerance under Pb stress.

Upon its discovery and implementation in plant protection, metalaxyl became one of the most important fungicides against Phytophthora infestans, but its efficiency has also been proven against other soil pathogens of the class Peronosporomycetes. The most important genus - Phytophthora comprises more than 150 plant pathogens, which cause significant losses in crop production or damage to natural plant associations. Many species of related genera Pythium, Phytopythium and Globisporangium have a similar ability as the species of Phytophthora. Those pathogens are able to quickly spread in wet soils by actively movable zoospores or in the air by means of zoosporangia; they are able to persist in an environment for long periods once they are introduced into the locality, having durability from their resting structures (chlamydospores, hyphal swelling and oospores). Metalaxyl has proven to be very efficient against these pathogens. However, shortly after its release, the rapid development of resistance against this compound was recorded in many species of the class Peronosporomycetes. Such easily developed resistance is due to the monogenic nature of the resistance, which also determines any anti-resistant strategies. The solution of this issue rests in the cautious use of metalaxyl, with consideration given to these strategies, and should be based also on precise information about the environment and the present pathogenic agents.

The environment is changing quickly and it is ever more burdened in connection with the greater needs of human society. This fact has increased efforts to improve the management of land and natural resources and the necessity to evaluate them. Land valuations become more important as the land consumption increases. Soil needs to be evaluated in the whole context of how its quality is affected and the values it provides. The concept of ecosystem services offers this holistic view. This paper defines ecosystem services (ES), the various linkages between soil properties, their functions and benefits, the assessment of soil quality using indicators and then briefly mentions EU environmental assessment methods and terms used in the context of ES. The article also mentions frameworks with which to assess and evaluate the soil quality that can be divided into two groups. The first group is comprised of a framework of indicators that describe the current state of the soil system assessment for evaluating the quality of the agricultural land. This is based on a detailed measurement of the terrain, a statistical analysis of soil databases or processing the status of specific threats to the soil. The second group is comprised of a framework of indicators focused on changes in the soil quality and applied soil management. These frameworks deal with the productivity of the soil in various systems of farming, compare agricultural systems or discuss the advantages of soil biota as indicators of soil quality in detail. Many of the designs of the soil quality indicators focus on the soil management in the context of a single discipline such as agriculture or water pollution. There are concepts for considering the soil quality in regional planning.

In order to increase the reuse of wastes and residues, as required by the Waste Framework Directive, the potential use of waste, residue and natural minerals as low-cost permeable reactive barrier (PRB) materials was investigated. The performance of a kitchen waste compost, sepiolite and steel slag was compared with that of volcanic slag, pumice and activated carbon in removing specific contaminants from landfill leachate. The experiments represented that the activated carbon removed 27% of the ammonium (NH4+), 75% of the chemical oxygen demand (COD) and 100% of the phosphate (PO43-), zinc (Zn2+) and nickel (Ni2+) from the landfill leachate. Volcanic slag exhibited removal efficiencies of 50% COD and 100% PO43- and pumice exhibited removal efficiencies of 20% NH4+, 27% Zn2+, 65% COD and 100% PO43-. The reactive materials were also checked for their potential in releasing unwanted constituents and represented different levels of the solute (e.g., PO43-, SO42-, NH4+) release. Among the reactives, sepiolite was found to be the reactive material reflecting a minor release (e.g., Zn2+, Cd2+ and Ni2+), but also delivering removal efficiencies of 40, 50, 65, 95, 97, 98, 98 and 100% for Ni2+, COD, Zn2+, SO42-, Cl-, F-, NH4+ and PO43-, respectively. The results show that the studied materials have the potential as reactives for PRB systems treating high strength contaminant plumes.

Soil aggregates have great effects on soil properties and soil functions. Mulching (organic inputs) has been known as a factor influencing soil aggregate stability. Our study aimed to reveal the causes of the higher stability of soil aggregates under organic mulches. The primary soil characteristics such as organic carbon (Cox), humus quality (E4/E6), potential wettability index (PWI), and aromaticity index (iAR) were determined. The Cox was measured using rapid dichromate oxidation, and E4/E6 was measured using the UV-Vis spectrophotometry. The PWI and iAR were determined according to the intensity of selected bands in diffuse reflectance infrared spectra. Results showed that mulched plots contained higher Cox content in aggregates in comparison with whole soil. This indicates that the carbon was stabilized within the aggregates and sequestrated into the soil. The iAR was significantly higher after using the organic mulches, the aliphatic components of the organic matter thus contribute more to the aggregates stabilization. The PWI of aggregates was found to be higher after applying these mulches than in soil. Organic mulches are therefore able to reduce the wettability of the aggregates and also to protect the aggregate from dispersion with water.

The widespread hillslope- and gully-erosion in Liaoning Province of Northeast China, pose serious challenges to the local agricultural production. Hillslope- and gully-erosion have typically been studied separately; however, there has been little investigation on the relationship of these two types of erosion. In this study, the coupling relationship of the hillslope- and gully-erosion from the perspectives of erosion intensity and land use, as well as the slope gradient, aspect, and shape, was analysed. The study employed remote sensing and geographic information system techniques, and the universal soil loss equation and kriging were used to perform a macroscopic analysis. The results showed that gully-erosion was more severe compared with hillslope-erosion in the study area. The cultivated land has the highest level of human activities, therefore, the most intense hillslope- and gully-erosion. The threshold slope gradients for the hillslope- and gully-erosion are 14° and 6°, respectively. Above the threshold of 6°, the slope gradient is no longer the primary factor affecting the gully-erosion. Sunny slopes have observably more hillslope-erosion than shady slopes, and the highest hillslope-erosion is observed on the south-southeast-facing slopes. The effect of the slope aspect on the gully-erosion should not be ignored, as evidenced by the considerable gully density of the east-northeast-facing slopes which is obviously higher than for slopes with other slope aspects. The highest hillslope-erosion amount and gully density occur on concave slopes, followed by convex and straight slopes, and straight slopes have little effect on the hillslope-erosion, but have a marked impact on the gully-erosion. The results of this work may serve as a scientific reference for the comprehensive control of soil erosion across a slope-gully system in Northeast China.

Several experimental methods have been developed to fractionate soil organic carbon (SOC) into functional sub-pools. However, which fractions had the potential to better reflect the SOC dynamics responding to fertilisation are still under discussion. Thus, we compared different SOC fractions (microbial biomass carbon, MBC; dissolved organic carbon, DOC; permanganate-oxidisable carbon, POXC; particle organic carbon, POC, and aggregation organic carbon fractions) and the soil respiration rate in a wheat-corn rotation field after 40 years of manure and N fertilisation in North China to search for the most sensitive SOC fractions to fertilisation. Manure increased the organic carbon (OC) contents of all the soil fractions (26.5 to 362.8%) and the POC (18.0 to 43.7%) and macro-aggregation percentages (3.0 to 4.4%), which indicated an increasing physical-protected aggregated OC fraction. N fertilisation alone slightly increased the OC contents of all the soil fractions and DOC percentage, but decreased the macro-aggregation OC percentage, which suggests the increasing possibility that the SOC is exposed to microbial communities causing a decreasing aggregation formation. However, when a high level of both the manure and N fertiliser were applied, the excessive N in the soil stimulates the soil microbial activity and decreases the SOC content comparing it to the same level of the manure fertiliser addition.

Land degradation caused by erosion processes is a widespread global problem. Rain simulators are one of the tools often used to determine the resistance of soils to erosion processes. The aim of this publication is to present the process of the construction and calibration of a small, portable field simulator which would be implemented in research studies designed to determine the changes in the soils' shear strength parameters in forested areas (in situ) caused by a change in soil moisture content achieved by the rain simulation. The constructed simulator consists of a metal frame, sprayers (with specific nozzles), a sediment funnel/tray made of metal, water and a sediment collector unit, a water tank and pump, and a set of rubber hoses, manometer, valves, reducers, adapters and other supplementary equipment. The calibration was carried out by using the pluviometric method. The choice of nozzles was based on the criteria of low water consumption (losses), the Christiansen uniformity coefficient (CU) and the possibility of achieving specific downpour intensities for the investigated area. The further calibration of the device consisted of determining the raindrop diameter and the distribution of the rainfall when the simulator is positioned on the slopes (7° and 15°). The achieved rain intensity was 1.7-1.9 mm/min, with a CU of 92.23-93.70% for the raindrop diameters (D₅₀) equal to 1.2 mm. The kinetic energy of the simulated rain (Ke) was 2.82∙10^-6 J. The constructed simulator proved itself to be in accordance with all of the given criteria, and it can successfully be implemented in research studies aimed at determining the resistance of forest soils to erosion processes, infiltration, and sediment yield.

Use of low-quality water for supplemental irrigation is expected to become soon a common practice in the Mediterranean area, where durum wheat is the main cultivated cereal. Breeding for salt stress tolerance may contribute to the improvement of wheat resilience to irrigation with brackish water. Various traits can be considered as indicators of salt stress tolerance, which include agronomical and physiological criteria. However, the complexity of salinity tolerance mechanisms, the G × E interaction and the lack of correlation between controlled and open field conditions causes uncertainty in the selection process. The present review highlights the main advantages and limitations of different agronomical and physiological traits used in screening for salt stress tolerance in wheat. Special focus is given to carbon and nitrogen isotope discrimination, that remains a bottleneck in breeding for salt stress tolerance. The use of different statistical tools to analyse data related to salt stress tolerance is also discussed in this review.

Soil thermal properties regulate the thermal and water balance and influence the soil temperature distribution. The aim of the current study is to present data on the changes in the thermal properties of Cambisols at different ratios between the water content and the air in the pore space under different vegetation covers in mountain regions. The undisturbed soil samples were taken from the surface soil layers under grassland, deciduous and coniferous forests in three experimental stations of the Forest Research Institute - Gabra in Lozen Mountain, Govedartsi in Rila Mountain and Igralishte in Maleshevska Mountain. The soil thermal conductivity (λ), the thermal diffusivity (α) and the volumetric heat capacity (C_v) were measured with the SH-1 sensor of a KD2Pro device at different matric potentials in laboratory conditions. The thermal conductivity of the investigated soils was also measured with the TR-1 sensor of a KD2Pro device at the transitory soil moisture in field conditions. An increase in the thermal properties with the soil water content was best pronounced for λ and depended inversely on the total porosity. As the total porosity increased with the soil organic carbon content and decreased with the skeleton content, the lowest value of λ was established in the surface horizons of Dystric Cambisols (Humic) in the experimental station in Govedartsi. The soil thermal conductivity increased with the depth under the deciduous forest (Gabra and Igralishte) due to the lower soil organic carbon content (SOC) and the total porosity. There were no such changes in the subsurface horizon under the grassed associations. The increase in the heat capacity with the water content depended on the SOC to less extent. In the horizons with a SOC of less than 1.5%, the changes in the thermal diffusivity over the whole range of wetness were 1.7 times higher than those with a higher SOC.