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Anti-coccidial effects of dietary chamomile against experimentally induced coccidiosis in broiler chickenOriginal PaperSleman Said Mohammed BeskiCzech J. Anim. Sci., 2023, 68(1):30-43 | DOI: 10.17221/160/2022-CJAS
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Characterisation of the HbSnRK2 gene family members and revealing specific HbSnRK2.2 functions in the stress resistance of the rubber treeOriginal PaperYu Zhang, Mingyang Liu, Dong Zhang, Meng Wang, Lifeng WangPlant Protect. Sci., 2023, 59(1):59-72 | DOI: 10.17221/48/2022-PPS SNF1-related protein kinase (SnRK2) is a critical positive regulatory factor in the abscisic acid (ABA) signalling pathway. However, the roles of the HbSnRK2 gene family members in the rubber tree, especially in response to stress, have not been thoroughly characterised. Here, we cloned six HbSnRK2 genes from the rubber tree. Based on the phylogenetic analysis, the HbSnRK2 family genes were divided into three groups. The motifs and intron numbers of HbSnRK2 were conserved. Analysis of cis-regulatory element sequences of all HbSnRK2 genes identified ABRE and TC-rich elements in the prompter of all the HbSnRK2 genes, illustrating that HbSnRK2 could be adjusted by the ABA and stress responsiveness. The qRT-PCR analysis showed that the expression patterns of the six HbSnRK2 genes differed in different tissues. The expression of these genes also differed under treatment with the plant hormone ABA, the HbSnRK2.2 gene was especially significantly expressed under the ABA treatment. Moreover, the HbSnRK2.2 gene responded to glyphosate, powdery mildew, heat stress and cold stress processes, which indicates that the HbSnRK2.2 gene plays an important role in phytohormone signalling and stress response in rubber trees. Taken together, the study provides valuable information to further define the role of the HbSnRK2 gene in rubber trees. |
Developing a decision-making model for improving the groundwater balance to control land subsidenceOriginal PaperMojtaba Zangeneh, Mahdi Sarai Tabrizi*, Amir Khosrojerdi, Ali SaremiSoil & Water Res., 2023, 18(1):55-65 | DOI: 10.17221/57/2022-SWR This study aimed to control land subsidence by improving the groundwater balance in the Varamin plain using the Groundwater Modelling System software and a multi-criteria decision model. For this purpose, aquifer level quantification and subsidence rate simulation were performed with the MODFLOW model and SUB package, respectively. The results showed a 6 m decrease in the aquifer level over a 5-year period and the subsidence rate in the central parts was 37 cm. Accordingly, the aquifer was evaluated by considering eight different restoration strategies based on reduced exploitation and artificial feeding. The results showed that the environmental criterion related to the subsidence adjustment index had the highest weight (0.27) and was introduced as the most important decision-making criterion. The evaluation of the results and priorities using the Complex Proportional Assessment (COPRAS) method showed that a 30% reduction in exploitation with artificial feeding is the best restoration strategy and can improve the subsidence rate and aquifer level by 36% and 76%, respectively, over a 5-year period (2024). |
Estimation of corn coefficients with vegetation indices using multispectral camera and droneOriginal PaperChristos Dimitrios Papanikolaou*, Maria Andreas Sakellariou-MakrantonakiRes. Agr. Eng., 2023, 69(1):36-47 | DOI: 10.17221/19/2022-RAE Optimum irrigation scheduling and new technologies are the key to the successful practice of modern agriculture and natural resources, such as water management. Α three-year research project was conducted at Velestino, Magnesia, Greece. The aim was to study whether vegetation indices can be used to estimate the crop coefficients of corn in order to apply an intelligent method of irrigation using drones in the future. The normalised difference vegetation index (NDVI), the soil-adjusted vegetation index (SAVI), the renormalised difference vegetation index (RDVI) and a new index [difference infrared – green vegetation index (DIGVI)] were calculated using multispectral photos from a camera adapted to a drone. Three different methods were applied to calculate the crop coefficients: (i) the water balance and the FAO Penman-Monteith reference evapotranspiration, (ii) the climatic data, (iii) the vegetation indices. The irrigation dose covered 100% of the crop water needs according to the soil moisture measurements and the single crop coefficient values. The statistical analysis and the simple linear regression method showed that the corn crop coefficients can be estimated when these indices are used as independent variables. |
Current nutritional guidelines in terms of the effect on gut microbiota and human health considering the WHO and FAO recommendationsReviewBarbora Rù¾ièková, Pavel KohoutCzech J. Food Sci., 2023, 41(1):1-7 | DOI: 10.17221/186/2022-CJFS The purpose of this paper is to evaluate current nutritional guidelines of modern diets based on medical and nutrition facts and their effect on gut microbiota and health, considering current recommendations of world authorities such as FAO and WHO. For this purpose, the first part is devoted to the impact of microbiota on human health, and special attention is committed to the effect of fibre on gut microbiota. The second part is dedicated to the fundamental division of diets and the evaluation of concrete nutritional guidelines of modern diets into microbiota and health, followed by the recommendations of global authorities. Modern diets include diets from the point of view of medical science (e.g. Mediterranean), promoted by nutritionists and authorities (e.g. Nordic) and by social trends (e.g. vegan). The evaluation summarises that high-fibre diets have tremendous benefits on human health. Diets with fresh, local and naturally fermented food positively impact the gut microbiota, hence human health (agrarian diets). The results of the review show that the nutritional guidelines associated with the lowest mortality are the Mediterranean with the Atlantic or Nordic diet, which is in line with the recommendation of the world authorities (FAO, WHO, UN). The low-fibre western diet with highly processed foods with no or very low levels of live bacteria appears to be high-risk in terms of preventing civilisation diseases with a negative impact on gut microbiota, which is in line with current FAO and WHO guidelines. |
Potential of moringa (Moringa oleifera) leaf powder for functional food ingredients: A reviewReviewLina Novi Ariani, Teti Estiasih, Wenny Bekti Sunarharum, Alfi KhatibCzech J. Food Sci., 2023, 41(1):8-20 | DOI: 10.17221/221/2022-CJFS
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A methodological proposal for the sociocultural valuation of ecosystem servicesOriginal PaperÓscar González-Yebra, José Ángel Aznar-Sánchez, Juan Francisco Velasco-Muñoz, Belén López-FelicesAgric. Econ. - Czech, 2023, 69(2):68-77 | DOI: 10.17221/298/2022-AGRICECON
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First cases of herbicide resistance of Tripleurospermum inodorum in the Czech RepublicOriginal PaperJaromír ©uk, Jakub Mikulka, Madhab Kumar Sen, Pavlína Ko¹narová, Kateøina Hamouzová, Josef SoukupPlant Soil Environ., 2023, 69(2):81-87 | DOI: 10.17221/427/2022-PSE Tripleurospermum inodorum (L.) Sch. Bip. is one of the most economically important and yield-reducing weeds in cereals in Europe. Random and systematic monitoring of this weed might provide an early warning for the farmers and slow down the pace of the evolution of herbicide resistance. This study aimed to identify resistant populations of T. inodorum in the Czech Republic and elucidate their possible resistance mechanism/s. Monitoring and screening of T. inodorum for herbicide resistance against acetolactate synthase (ALS) inhibitors and synthetic auxins was carried out. Greenhouse experiments and molecular-genetics studies were conducted to characterize the resistance. While all the tested populations were found to be susceptible to synthetic auxins, two populations (MATIN 2 and MATIN 4) were found to be resistant against tribenuron (ALS inhibitor). However, their cross-resistance to florasulam was not confirmed. The resistance mechanism detected was the target-site substitution of Pro197 at the ALS gene. The two populations carried different point mutations: Pro197Ser (MATIN 2) and Pro197Gln (MATIN 4). This is the first study in the Czech Republic to identify the survival mechanism in T. inodorum for resistance to ALS-inhibiting herbicides. Our results from this research will provide basis for resistance management in T. inodorum in the Czech Republic and other countries. |
Molecular mechanism of drought stress tolerance in barley (Hordeum vulgare L.) via a combined analysis of the transcriptome dataOriginal PaperMostafa Alamholo, Alireza TarinejadCzech J. Genet. Plant Breed., 2023, 59(2):76-94 | DOI: 10.17221/69/2022-CJGPB One of the main issues addressed by phytology in recent years has been plant tolerance mechanisms for abiotic stress. No combined analysis has been made to identify the genes involved in drought stress tolerance. The meta-analysis of microarray data related to drought stress was analysed by the R software packages and showed 3 029 upregulated genes and 3 017 downregulated genes. The upregulated genes were mostly related to the drought tolerance protein, abiotic stress response, and the Cys2His2 Zinc Finger Transcription Factor (C2H2 zinc finger TF). The downregulated genes were mainly related to the late embryogenesis abundant protein, abiotic stress response, and the basic leucine zipper (bZIP) TF. The common gene ontology (GO) terms in the upregulated and downregulated genes were mainly related to the metabolic process, response to stimulus, cellular metabolic process, and photorespiration. The up and down meta-differential expressed genes (meta-DEGs) mainly belonged to the those following Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including: the biosynthesis of secondary metabolites, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signalling pathway, and RNA degradation. Moreover, in the upregulated and downregulated genes, the TFs with a high percentage mainly belonged to the Teosinte branched1/Cincinnata/proliferating cell factor (TCP), basic helix loop-helix (bHLH) and bZIP. Next, the hub upregulated genes were mainly related to the thiamine biosynthesis protein thiC, 4-hydroxyphenylpyruvate dioxygenase, ribose-5-phosphate isomerase precursor and heat shock protein. The hub downregulated genes were mainly associated with the elongation factor Ts, aldehyde dehydrogenase, and trigger factor. Finally, the data from the present meta-analysis were compared with previous studies on the qRT-PCR results and their up and down expressions were confirmed. Based on the findings of the current study, novel insights into the drought stress molecular response can be provided and various candidate genes can be introduced for barley drought stress tolerance breeding. |
QTL localisation of seed-related traits in Tibetan hulless barley based on a high-density single-nucleotide polymorphism genetic mapOriginal PaperYan Wang, Xiaohua Yao, Youhua Yao, Yixiong Bai, Likun An, Xin Li, Yongmei Cui, Kunlun WuCzech J. Genet. Plant Breed., 2023, 59(2):95-108 | DOI: 10.17221/74/2022-CJGPB The effective use of high-quality and high-yielding germplasm resources is of great importance for the development of hulless barley varieties. Therefore, the search for such resources has long been a goal of the breeding community. In this study, a genotyping-by-sequencing (GBS) analysis was performed on an F2 (Nierumuzha × Kunlun 10) population. A high-density genetic map of hulless barley was constructed, which contained 1 475 efficient single-nucleotide polymorphism markers with 7 052 bin markers. The total length of the seven chromosomes was 1 139.4 cM, with an average marker distance of 0.59 cM. Based on this high-density linkage map, a total of 54 quantitative trait loci (QTLs) related to the seed traits were detected, including seed colour (SC), thousand kernel weight (TKW), seed average area (SAA), seed perimeter (SP), seed length (SL), seed width (SW), seed length/width (SLW), seed diameter (SD), and seed circular degree (SCD). These QTLs explained 3.32–35.73% (mean = 11.45%) of the phenotypic interpretation, including 24 main QTLs and 30 epistatic QTLs. A total of 24 candidate genes were identified within the QTL region, including one SC-associated transcription factor (ANT1), two TSW-related genes, a transcription factor (NAC021), a gene associated with the non-homologous end joining (NHEJ) pathway (ku70), three SAA-associated genes (LOL2, NAC021, TSK), two SL-associated genes (MADS21, MADS4), six SW-associated genes (FIP1, NAC021, DREB 1A, HVA22A, CYP78A6, SAUR71), five LW-related genes (NAM-B2, CRY1, LHY, CYP710A1, WRKY72), two SP-related genes (SKIP11, TCP18), two SD-related genes (NAC021, SKIP8), and three SCD-related genes (MYB1R1, RAX3, NAC100). These genes are involved in the regulation of the cell development, material transport, signal transduction, and plant morphogenesis and play an important role in the regulation of agronomic traits in hulless barley. The high-density genetic mapping and QTL identification of the seed traits in hulless barley provide a valuable genetic resource and the basis for further molecular marker-assisted selection and genomic studies. |
Effect of debranching enzyme hydrolysis and microwave treatments on the resistant starch enrichment of breadfruitOriginal PaperThanh Le, Trang Tran, Tuyen Kha*Czech J. Food Sci., 2024, 42(1):1-9 | DOI: 10.17221/136/2023-CJFS
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The impact of geopolitical risk on agricultural commodity pricesOriginal PaperKristína Hudecová, Miroslava RajèániováAgric. Econ. - Czech, 2023, 69(4):129-139 | DOI: 10.17221/374/2022-AGRICECON
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Analysis of global warming potential: Organic vs. conventional tomatoesOriginal PaperAlessandro Scuderi, Giuseppe Timpanaro, Mariarita CammarataAgric. Econ. - Czech, 2023, 69(7):267-275 | DOI: 10.17221/104/2023-AGRICECON
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Inorganic and organic foliar fertilization in olivesReviewSavros Sotiropoulos, Christos Chatzissavvidis, Ioannis Papadakis, Viktor Kavvadias, Christos Paschalidis, Chrysovalantou Antonopoulou, Antonia KorikiHort. Sci. (Prague), 2023, 50(1):1-11 | DOI: 10.17221/69/2022-HORTSCI The application of nutrient solutions to the foliage of plants is an alternative means to fertilise crops. Foliar fertilisation is used to overcome the disadvantages of soil applications. Variable plant responses to these foliar fertilisation spray solutions have often been described in the literature. However, knowledge about the penetration mechanisms, the role of a leaf-applied inorganic or organic solution, and the results obtained by its application is still limited. The complex character of the factors determining the effects of sprays hinders the development of suitable foliar fertilisation strategies, applicable under variable local conditions and for various plant types. This review describes some basic factors involved in the penetration process of foliar sprays in olive (Olea europaea L.) trees. Chemistry, leaf penetration, and plant nutrition principles will be merged with the aim of clarifying the constraints, opportunities, and future perspectives of sprays to cure olive tree nutrient deficiencies and, hence, both increase the yield and improve the fruit quality. |
A review on seasonal succession and management of key insect- pest infesting tomatoReviewSoniya Dhanda, Surender Singh Yadav, Anil Jakhar, Sonu KumariHort. Sci. (Prague), 2023, 50(1):12-24 | DOI: 10.17221/31/2022-HORTSCI Tomatoes have become one of the most significant and profitable vegetable crops farmed for fresh market and processing in tropical and subtropical parts of the globe and they are an important element of our human nutrition. Tomatoes, like other vegetables, are more susceptible to insect pests and illnesses than other crops, owing to their sensitivity and softness. Insect pests are among the many causes that cause low tomato yields includes fruit borer, jassid, white fly, aphid and leaf miner. On the other hand, enough understanding about seasonal abundance of insect pests is required for the formulation and implementation of an appropriate, effective, and timely pest management approach. The current demand focuses not only on the use of various eco-friendly chemical groups, but also the employment of unique modes of action to provide sufficient control of insect pest populations. So, this work reviews and presents a pool of research on seasonal succession and management of key insect pests of tomato. |
The impact of urine storage methods on the results of quantitative bacterial culture in dog and catOriginal PaperCJ Lien, SL WangVet Med - Czech, 2023, 68(3):116-121 | DOI: 10.17221/111/2022-VETMED Quantitative bacterial culture (QBC) is the gold standard for determining urinary tract infections. However, the majority of urine samples were sent to a lab for further bacterial culture. Therefore, the storage condition was vital to maintain the quality and accuracy of the urine samples. The main objective of our study was to examine the urine QBC outcomes of (1) immediate culture, (2) culture after urine sample storage at ambient temperature for 24 h, and (3) culture after urine sample storage at 4 °C for 24 hours. There were 49 feline samples and 30 canine samples included in this study. All QBC samples kept at ambient temperature and refrigerator were consistent with immediate QBC in cats. Eight positive results from immediate QBC were in accordance with refrigerated results in dogs. There were ten positive results in the room-temperature sample with two false-positive results. Our study showed that storing conditions at room temperature or refrigeration for 24 h does not impact the results of QBC in cat urine samples. For dog samples, chilled samples have a higher accuracy rate than room temperature samples, although the overall agreement was still satisfactory. |
Operating performance of manual, semi-automatic, and automatic tractor guidance systems for precision farmingOriginal PaperPaola D'Antonio, Andi Mehmeti, Francesco Toscano, Costanza FiorentinoRes. Agr. Eng., 2023, 69(4):179-188 | DOI: 10.17221/5/2023-RAE Precision agriculture is increasingly relying on tractor auto-steer systems to boost productivity and optimize crop inputs. Identifying field variations and performance, on the other hand, is necessary for giving site-specific recommendations. This study reports the field operating performance indicators of manual (MG), semi-automatic (SG), and automatic (AG) tractor guidance for weed control in wheat production in Southern Italy. Performance indicators include effective worked area, overall working time, effective field capacity, field efficiency, fuel consumption, and product usage. The SG tractor guidance working times were similar to the MG, but with significant savings in the herbicide spray solution and work quality. In terms of all parameters examined, the AG outperformed the SG and MG. The AG was 54% faster than the MG, resulting in an increased area worked and effective field capacity of 5 and 46%, respectively. The total time (effective time plus non-productive time) was reduced by 28%, while overlapped areas by 88.9%. Herbicide and fuel input was reduced by 30 and 11.5%, respectively. A streamlined environmental analysis indicated that AG could reduce the energy and carbon intensity of the one-time weed control process by 25 and 27% for each hectare. Our results confirm that auto guidance provides numerous benefits (e.g., machining uniformity, increased work quality, reduced resource use, and reduced environmental burdens), supporting the larger goal of agricultural production sustainability. |
Research on the promotion effect and mechanisms of digital empowerment of food enterprisesOriginal PaperPenglong Li, Xuan YeAgric. Econ. - Czech, 2024, 70(2):60-72 | DOI: 10.17221/271/2023-AGRICECON
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Meta-analysis of genetic parameters for productive traits in Nile tilapia (Oreochromis niloticus)Original PaperElizangela Zayana Lima D'suze, Nandrya Hayne Santos de Lima, Denise de Assis Paiva, Nilsa Duarte da Silva Lima, Thalles Ribeiro Gomes, José Teodoro de PaivaCzech J. Anim. Sci., 2024, 69(5):200-208 | DOI: 10.17221/6/2024-CJAS The advances in the production traits of Oreochromis niloticus are due to the development of genetically superior broodstock animals. Understanding the genetic variability of fish stocks is crucial to any animal breeding program. The aim of this study was to perform a meta-analysis of genetic parameters that considers the variability between studies for economically important traits in the Nile tilapia (Oreochromis niloticus) production. The final dataset from literature review contained 226 estimates of heritability and 53 estimates of genetic correlation across 37 peer-reviewed articles published between 2002 and 2022. Weighted genetic parameter estimates were obtained by fitting a random effect model for all traits studied. Heterogeneity across studies were assessed using the Q statistic and I2 index. Heritability estimates ranged from low to moderate magnitude, indicating that a greater response to selection is expected for body length (0.37), body weight (0.29), body width (0.28), and daily weight gain (0.28), compared to the other traits, body height (0.25) and fillet yield (0.19). Genetic correlations ranged from moderate to high magnitude (0.47 to 0.94), which suggests that part of expression of traits is regulated by the same gene groups. The meta-analysis study provided reliable genetic parameter estimates and these findings may be served as the baseline reference for new Nile tilapia (Oreochromis niloticus) breeding programs. |
Application of the physical properties of local cowpea varieties in the development of a multi-variety cowpea cleanerOriginal PaperBabatunde Olusola Adetifa, Elizabeth Ayobami Olumomi, Taiwo Moses Samuel, Ayoola Abiola BabalolaRes. Agr. Eng., 2024, 70(2):82-91 | DOI: 10.17221/51/2023-RAE Cowpea, an annual legume widely grown and consumed in Nigeria, has been observed to contain between 27–33% impurities when freshly harvested and threshed. This poses a threat to humans when consumed and in large-scale agricultural processing. Therefore, this study is aimed at developing and evaluating the performance of a multi-variety cowpea cleaner (MVCC). Using standard methods, some selected engineering properties of the cowpea varieties were examined and used in the design of the MVCC. The cowpea had a moisture content of 8–14%, depending on the locations and varieties. Other properties investigated include the length, width, thickness, sphericity, geometric mean diameter, unit volume, arithmetic mean diameter, aspect ratio, surface area, unit weight, true density, terminal velocity, and angle of repose. The MVCC comprised the hopper, winnower, cleaning unit, fan assembly, and frame. The performance of the MVCC was also evaluated. The efficiency of separating good products of honey, drum, and Sokoto White beans was 95, 91, and 84%, respectively, while separating bad products was 87, 94, and 96%, respectively. |
Studies on the growth, immunomodulation and gut morphometry of Labeo rohita fed pectinOriginal PaperHumaira Sehrish Hashmi, Noor Khan, Khalid Javed Iqbal, Mahroze Fatima, Khalid Mahmood Anjum, Sumaira Abbas, Muhammad Awais, Sadia Nazir, Muhammad Asghar, Mahanama De ZoysaCzech J. Anim. Sci., 2023, 68(4):178-187 | DOI: 10.17221/132/2022-CJAS
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The effect of irrigation treatments at different development stages on the bioactive components of sunflower cakeOriginal PaperErhan Göçmen, Sıla Barut Gök, Yasemin ErdoğduPlant Soil Environ., 2023, 69(4):179-187 | DOI: 10.17221/440/2022-PSE The aim of the study was to determine the effect of water deficiency at different development stages on the bioactive content and phenolic compounds in sunflower (Helianthus annuus L.) cake, the residue left after oil is extracted from sunflower seeds. A sunflower genotype was randomly planted in a complete block design with eight different combinations of irrigation (T1–T8) by increasing the available soil moisture measured at different plant growth stages (vegetative, flowering and grain formation). Results indicated that the total phenolics of extracts varied between 1.03–2.03 times more than under drought stress (T8). The antioxidant capacity response of seed cake was 14–39% lower than under drought stress. Irrigation treatment, except in the grain formation stage, was found to enhance the biosynthesis of phenolic compounds such as vanillic and caffeic acids. Irrigation only in the grain formation stage induced the accumulation of phenolic compounds such as coumaric acid and rutin hydrate. The present study established that residues resulting from oil extraction could be converted to a polyphenol-enrichment agent for food systems by manipulating the irrigation treatments. |
Thinning effects on growth and occurrence of rotting in aspen standsOriginal PaperAlina Nasibullina, Marieke van der Maaten-Theunissen, Ernst van der Maaten, Holger Fischer, Sven WagnerJ. For. Sci., 2023, 69(12):525-538 | DOI: 10.17221/103/2023-JFS Poplar species such as aspen (Populus tremula L.) play a very important role in the forest formation process not only in Eastern European regions. Unfortunately, such aspen stands are often severely affected by fungal diseases, causing mainly core rot. In this study, the indirect effects of thinning on the phytosanitary condition of aspen by promotion of tree growth were investigated. Two thinning methods, manual (thinning from below) and mechanical thinning (schematic), were applied to young stands dominated by Eurasian aspen to study their effects on tree growth and health. All trees were measured at breast height and diameter frequency distribution was determined twice, i.e. three and 24 years after the beginning of the experiment. In addition, during the second measurement, tree-ring samples were obtained from individual trees to evaluate growth and wood decay damage. Neither manual nor mechanical thinning of aspen significantly increased its growth at the stand level, but positive effects on individual trees were observed in plots where mechanical thinning was applied. The thicker the trees, the less decayed they were. The analysis suggests that thinning in general should not be used to increase stand production, but the positive effects of mechanical thinning on individual aspens can be recommended to promote the growth of individual vigorous trees. |
Estimation of coffee shelf life under accelerated storage conditions using mathematical models – Systematic reviewReviewMaría Alina Cueva Ríos, Frank Fernández Rosillo, Lenin Quiñones Huatangari, Eliana Milagros Cabrejos BarriosCzech J. Food Sci., 2023, 41(2):92-102 | DOI: 10.17221/163/2022-CJFS
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Corporate governance, ownership concentration and performance of European agricultural companies: New empirical evidenceOriginal PaperGraþiela Georgiana Noja, Mirela Cristea, Nicoleta Sîrghi, Oana-Ramona Socoliuc Gurițã, Ioana Vãdãsan, Daniel CîrciumaruAgric. Econ. - Czech, 2023, 69(4):151-161 | DOI: 10.17221/78/2023-AGRICECON
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Mapping of genomic regions associated with dwarfing and the determinate growth habit in horsegram (Macrotyloma uniflorum)Original PaperMala Ram Modi, Megha Katoch, Nisha Thakur, Manisha Gautam, Sunny Choudhary, Rakesh Kumar ChahotaCzech J. Genet. Plant Breed., 2023, 59(4):196-204 | DOI: 10.17221/119/2022-CJGPB Horsegram (Macrotyloma uniflorum) – an important, self-pollinated food legume, however due to limited genomic and genetic resources the genetic improvement could not be achieved as compare to other major legumes. Our work aims at finding novel microsatellite markers and their use for the construction of a linkage map from 157 individuals of F9 recombinant inbred lines (RILs) of horsegram. The determinate growth habit and plant height are important traits for its suitability for different cropping systems. The genotypic data were generated by screening 2 395 molecular markers, of which 600 (25.05 %) polymorphic markers were selected. Two-hundred eighty-seven (287) markers were mapped on ten linkage groups (LGs) at a log of odds (LOD) of 3.5 straddling 796.76 cM with 2.78 cM of marker density. For the identification of the quantitative trait loci (QTLs), the phenotypic data recorded on the RILs for the plant height and growth habit were analysed using the statistical tools JoinMap®and Windows QTL cartographer, based on the composite interval mapping (CIM) technique. Across the ten linkage groups, we detected four QTLs (LOD ≥ 2.5) for four traits. All the traits were major QTLs as indicated by the percentage of phenotypic variance (PVE) (≥ 10%) that ranged from 13.5% to 40.3%, therefore, this is very important information which can be used in marker-assisted selection (MAS). The present genomic information generated in this orphan crop, thus, provides the base for genetic improvements by devising molecular breeding strategies. |
Assessment of heavy metals in soil, oilseed rape (Brassica napus L.) and honeyOriginal PaperAtanas Atanasov, Ivaylo Hristakov, Gergana Kuncheva, Milan Koszel, Veselin DochevPlant Soil Environ., 2023, 69(8):400-407 | DOI: 10.17221/265/2023-PSE The aim of this study was to determine the heavy metals concentrations in soil, inflorescences of Brassica napus and rapeseed honey sampled from some regions of north-eastern Bulgaria. Thirteen locations were selected for experimental studies. The soils, plants and honeys samples were taken from conventional beekeeping areas away from major industrial pollutants. The median amounts of elements in the soil samples (mg/kg DW) are 1.1246 for Fe(s), 0.7048 for Al(s), 0.5636 for Pb(s), 0.1658 for Cu(s) and 0.0148 for Zn(s). The median amounts of heavy metals measured in the inflorescences of Brassica napus (mg/ kg DW) are 5.5430 for Fe(p), 2.9095 for Zn(p), 1.3225 for Pb(p), 0.2593 for Cu(p) and 0.2105 for Al(p). The median heavy metals concentrations in tested honey (mg/kg DW) are 1.0026 for Fe(h), 0.1849 for Al(h), 0.1832 for Pb(h), 0.1250 for Zn(h), 0.0702 for. Cu(h). The relationship between the heavy metal in soil, plants and honey was investigated using the Spearman’s rank correlation coefficient. Significant differences in the concentrations of Fe(s), Al(s) and Pb(s) in soils, Fe(p) and Zn(p) in the plant samples, Fe(h) in honey samples from the different locations were found. The heavy metal content tested in honey did not pose a risk to human health. |
The importance of slaughterhouses in monitoring the occurrence of tail biting in pigs – ReviewReviewM Svoboda, N Hodkovicová, A Siwicki, W SzwedaVet Med - Czech, 2023, 68(9):349-358 | DOI: 10.17221/85/2023-VETMED Tail biting in pigs represents a very serious problem in modern pig farming, particularly with the intensification of the industry. It is considered a multifactorial syndrome and can be caused by various factors, leading to significant economic losses through reduced weight gain and partial or total condemnation of slaughtered carcasses due to secondary bacterial infections. The aim of this article is to summarise the current knowledge regarding tail biting in pigs, with a primary focus on the use of slaughterhouses for evaluating tail lesions and monitoring their prevalence. The introduction addresses the factors influencing the incidence of tail biting in pig farms and prevention strategies. Subsequent sections cover topics such as tail docking, the negative effects of pig tail biting, the advantages and drawbacks of examining tail lesions in slaughterhouses, and the methodical procedure for evaluating such lesions. Additionally, the article discusses the relationship between tail lesions and meat inspection findings, as well as the prevalence of tail lesions in various European countries. |
