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Anthracnose leaf spot caused by Colletotrichum gloeosporioides is an important disease of apples, resulting in serious damage to the fruit production. In this paper, the pathogenic and physiological characters of sixteen isolates and the wild isolate "Stj16" were studied. In the current study, we generated C. gloeosporioides strains expressing green fluorescence by introducing a GFP gene via an Agrobacterium tumefaciens-mediated transformation (ATMT). To confirm the subcellular localisation of the A2799 gene, an A2799gfp fusion expression mutant was constructed. After observation of the fusion expression, the A2799gfp fusion expression protein was located in the peroxisomes of the cell. The pathogenicity results showed that the mutants A4204, M44, A1919, A3638 and A1598 lost the pathogenic capability and virulence, however, the virulence of the mutants A1764, A439, A3885, G1183, A3144, A1649 and A2675 increased significantly to the apple fruits compared to the wild isolate "Stj16". The biological study indicated that a Rose Bengal Agar (RBA) medium decreased the mycelium growth, but it can increase the sporulation for most of the isolates. The mutant A4204 does not grow well at pH 4.0 and pH 8.0, and mutant M44 just has the optimum growth at pH 8.0, and a 12 h light and 12 h dark condition stimulates the sporulation for most of the tested mutants, but the A1764 mutant more sporulated at regular dark conditions. All the mutants and "Stj16" grew vigorously at 25 °C-30 °C, for "Stj16", it produced the highest number of conidia at 30 °C compared with the other temperatures. Based on the biological study, we found the best growing and sporulation conditions for all the tested isolates. The information generated in the present study will facilitate molecular research on this devastating fungus.

The experiment was established in the Pomological Orchard of The National Institute of Horticultural Research in Skierniewice in a system of randomized blocks. The aim of the experiment was to investigate the impact of innovative organic fertilizers: BioIlsa, BioFeed Ecomix, biostimulator Ausma and mycorrhizal inoculum Mykoflor on the fine roots growth characteristics of 'Vanda' sweet cherry trees in comparison with NPK mineral fertilization. The experiment involved five combinations, in three repetitions of three trees each, treated with tested preparations. The study assessed the influence of fertilization on the lifespan of the roots, the depth of their formation, their diameter and survivorship using minirhizotron camera. The highest numbers of roots were found in the treatment where the plants were fertilized with NPK and the lowest following the use of the biofertilizer BioFeed Ecomix. The longest lifespan was shown by the roots of the trees treated with BioFeed Ecomix - 347 days, and the shortest - by those fertilized with the Ausma - 225 days. The lifespan of the roots increased with their diameter. The roots that lived the longest had a diameter in the range from 0.9 to 1.0 mm - 568 days, and the shortest-living were the roots with a diameter smaller than 0.3 mm - 238 days. The roots that formed in late autumn and winter had the shortest median lifespan of 159 days, while the roots formed in the spring where characterized by the longest lifespan of 300 days. The lifespan of the roots formed close to the soil surface was the shortest - 225 days, while that of the roots formed at a depth of 10 to 20 cm was the longest - 326 days. Biological origin, organic nitrogen rich fertilizers positively influence on fine roots lifespan and longevity. Mineral fertilization increases number of new formed roots.

Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.

For 2 years (2019-2020), a field experiment to test the activities of different fertilisation schemes on the yield, colour and health status of five different grass mixtures for football pitches was conducted. Two grass mixtures were composed of different varieties of perennial ryegrass, one mixture was composed of varieties of perennial ryegrass and Kentucky bluegrass, one mixture consisted of the species Lolium perenne, Festuca rubra, Festuca arundinacea and Poa pratensis, and the seeds of only one variety of perennial ryegrass were sowed in one plot. Three different fertilisation schemes were included in the experiment. The first scheme (A) contained an inorganic fertiliser with added soil improvers, the second scheme (B) included an organic fertiliser with added soil improvers and the third scheme (C) was composed of an inorganic fertiliser. According to this study, the grass yield largely depends on the soil temperature, amount of precipitation and soil water content, and the occurrence of the fungus Laetisaria fuciformis is influenced by the fertilisation scheme, as the % infection with the fungus was highest when organic fertilisers with low % nitrogen was used. The selection of fertilisers is seen as an important factor for the turfgrass colour.

In temperate climatic conditions, plantations of poplar and willow species provide sustainable production of biomass. Short rotation coppice plantations on agricultural lands have a great potential to increase the amount of biomass available for the production of biofuels, bioenergy and bioproducts. We studied and measured the growth of poplar and willow clones in two experimental research sites in Batys and Astaninskiy in the steppe zone of Astana in northern Kazakhstan. We measured tree heights, stem diameters at breast height and crown diameter. The mean height of 6-years-old trees of the "Kazakhstanskiy" hybrid poplar was 4.03 m in the Batys site, which is 1.6 m less than the height of poplars from the Astaninskiy experimental site that were measured in autumn. The results of this study show different reactions of selected clones/cultivars of poplar and willow to climatic conditions of northern Kazakhstan as well as their usability for biomass production in plantations under limiting hydrological conditions.

Currently, it is difficult to identify correct data on wood sources and their consumption in the Czech Republic. Official statistics of wood production are relatively limited to obtaining data that accurately captures the objective wood production, which would enable the observation of the direction or consumption of particular tree species or products. This uncertainty is then transferred to the wood flow for industrial and energy processing, and it is not possible to compose a detailed picture of how much wood and which sources enter into the wood processing and how large the total material flow is. The aim of this paper is to present an option for how to determine this lack of valid information that enables clear outcomes necessary for decision making by means of the recalculation of wood sources consumed in the Czech Republic, using the data of processing capacities and foreign trade. The reverse model better expressed a conversion value of roundwood into the products and allowed optimal approximation in the wood sources assessment.

Calculating the factor of safety (FS) as slope stability factor is necessary to prevent environmental damage. Therefore, this paper aims to (i) calculate FS using the limit equilibrium approaches and Slide software and (ii) investigate the stability of slopes according to calculated FS (Janbu and Bishop methods) and status of different sites in the study area (Hyrcanian forest of Iran). Six landslides were selected along the forest road by a field survey. Landslide dimensions including length, width, and height were measured using meters. Slope gradients were measured using the Suunto clinometer. The Slide software was used to evaluate the stability analysis of slopes. According to laboratory tests on soil samples the average of the liquid limit was recorded as 58%. The results of the direct shear test showed that the rate of soil cohesion (c) and coefficient of friction angle (φ) decreased with an increase in moisture content. According to the results, the factors of safety for landslide sites (Sites 1-6) were calculated to be 1.3, 0.65, 0.76, 0.55, 1.19 and 1.51, respectively. These calculated FS can accurately determine the slope status in terms of slope stability. According to the software classification, the status of Site 1 is "susceptible to landside". Sites 2, 3 and 4 are "very high risk". Also, the status of Sites 5 and 6 are "high risk" and "stable", respectively. The instability of the slopes in studied sites is related to the drainage system (lack of culverts or ditches) as well as marlstone as bedrock. According to the calculated FS in different sites and comparison of the obtained results with the real conditions of sites, it can be concluded that the slope stability analysis in the Slide software is very accurate and it can be used to determine the factor of safety under different conditions in terms of morphology, hydrology and soil mechanics.

Lodgepole pine (Pinus contorta Douglas ex Loudon) was distributed from its natural range in western North America to different destinations, primarily to Europe (Scandinavia, British Islands), South America (Chile, Argentina), and New Zealand. It is used for its superior timber production and resistance to environmental conditions. This literature review paper consists of 150 references and presents a summary of research results dealing with the lodgepole pine potential in general with a specific focus on Europe from 1910 to 2022. It summarizes the importance, taxonomy, biological and ecological characteristics, site requirements, production and silviculture, risks and pests, as well as the potential of this tree species for forestry and the wood industry in relation to global climate change. Pinus contorta also has a considerable potential in Central Europe, especially at extreme sites with strong anthropogenic impact and in polluted regions. This tree species is very resistant to climatic factors and extreme events compared to other coniferous tree species. Moreover, its annual increment reached from 3 m³.ha^-1.yr^-1 on reclamation sites to 18 m³.ha^-1.yr^-1 in favourable environmental conditions. On the other hand, caution must be taken for possible invasive behaviour outside its natural range. Its importance can increase with ongoing climate change and the decline of native tree species.

Young trees (saplings) of the Norway spruce (Picea abies [L.] Karst.) regenerating populations were analysed on 7 plots in the Šumava Mts. (Bohemian Forest), on 5 plots in the Jeseníky Mts. (Eastern Sudetes), and 1 plot in the Krkonoše (Giant Mts.). All 13 plots were located at the forest altitudinal (vegetation) zones of natural Picea abies stands. Each selected tree was characterized by microscopic features of the first-year needles. The free-hand needle cross-sections were prepared from three needles of each tree and measured by digital microphotos. The following needle characteristics were measured: width, thickness, and vascular bundle diameter. Each population was described by variability of these parameters. Populations were classified based on the data set. Two artificially planted populations were most different. Populations resulting in natural stands have different phenotype variability, possibly as a result of the parent stand history: two extreme examples are Eustaška locality (Jeseníky Mts.) with no known disturbance, and Trojmezí locality (Šumava Mts.), where wind and bark beetle disturbances were repeatedly recorded.

Endophytic fungi are widespread in plants and affect the host fitness and population size. We found 12 fungal taxa in C. secalina, a critically endangered species in several European countries, at two study sites in Poland. The most frequently occurring fungal taxa were Colletotrichum destructivum and Acremonium sp. Both taxa were found in half of the examined tussocks. The highest number of fungal taxa was noted in the C. secalina plants growing in the roadside area, where 7 of the 12 identified fungal taxa occurred. These fungi, inhabiting leaf tissues, are known for their pathogenicity but no visible symptoms of any diseases were observed on C. secalina leaves. This suggests that these fungi are latent pathogens.

The objectives of this study were to analyse the relationship between the contents of elements and free amino acids (AAs) in fronds of As-hyperaccumulator Pteris cretica cv. Albo-lineata (PC) and non-hyperaccumulator Pteris straminea (PS) during reversible senescence. The time-course effect on senescence was also investigated. The two ferns were grown in a pot experiment with soil containing 16 mg As_total/kg soil for 160 days. The contents of elements and AAs in both ferns and in individual sampling periods differed. The highest accumulation of elements and AAs was measured in PS fronds after 83 days; however, the accumulation of As, Ca, Cu, Fe, Mg, P and asparagin in PC fronds was highest after 160 days. The results of principal component analysis showed more rapid senescence of PS compared to PC. This was caused by changes in the relationship between the contents of elements (cofactors of metalloenzymes, stress metabolites) and AAs (transport of NH₂ group and stress metabolites). The hyperaccumulator plant (PC) was more resistant than the bioindicator plant (PS) to the conversion from reversible to irreversible senescence.

To investigate the effect of microbial inoculum on soil heavy metal immobilisation, pot experiments were conducted with paddy soils contaminated by cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg), respectively. The results showed that the inoculation of Rhodopseudomonas palustris was more effective in the immobilisation of Pb and Cd in soils than the composite of R. palustris and Bacillus subtilis. Interestingly, a lower dosage of inoculum immobilised significantly more heavy metals than the higher dosage, potentially due to the competition of bacteria with limited nutrients. The heavy metal contents in rice grains also supported this finding, as less Pb and Cd were accumulated under the lower dosage. However, there were limited effects of microbial inoculations on the immobilisation of Hg and As. In general, our study indicated the effectiveness of R. palustris in immobilising Pb and Cd in soils and highlighted the importance of determining the optimal dosage of inoculum in bioremediation.

Sustainable cropping systems require efficient usage of fossil energy. This study performed on a long-term field experiment in the Pannonian Basis investigated the energy efficiency of four tillage systems (mouldboard plough (MP), deep conservation tillage (CT_d), shallow conservation tillage (CT_s) and no-tillage (NT)) for sugar beet and soybean production, taking fuel consumption, total energy input (made up of both direct and indirect inputs), crop yield, energy output, net-energy output, energy intensity and energy use efficiency into account. The input rates of fertiliser, chemical plant protection, and seeds were set constant across years; whereas measured values of fuel consumption were used for all tillage treatments. NT required a considerably lower energy input than MP and CT_d as no fuel is needed for tillage and just slightly more fuel for additional spraying of glyphosate. Anyhow, the energy efficiency parameters did not differ between tillage treatments, as theses parameters were mainly determined by energy output, which was considerably higher than the energy input. However, year effects on the energy efficiency were observed for both crops. Nitrogen fertilisation and diesel fuel consumption were identified as the most energy-intensive inputs. Consequently, the energy input for sugar beet was higher than that for soybean, which was identified as a low-input crop. But sugar beet attained a more than 4 times higher net-energy output, a 2.5 times higher energy use efficiency, and an energy intensity for yield production of less than 3 times those of soybean.

Small field plot experiments were carried out at the testing station of the Central Control and Testing Institute in Agriculture in Veľký Meder (Slovakia) in the experimental years 2014/2015, 2015/2016 and 2016/2017. Selenium salts in the form of sodium selenite and sodium selenate were applied in growth phases: end of tillering (BBCH 29) and flag leaf ligule and collar visible (BBCH 39). The effect of experimental years 2014/2015, 2015/2016 and 2016/2017 on the yield of wheat grain was not statistically significant within the observed variants. The achieved mean yields were in the range from 10.06 ± 0.81 to 11.07 ± 0.29 t/ha in 2014/2015, from 9.82 ± 0.54 to 10.32 ± 0.10 t/hain 2015/2016 and from 11.23 ± 0.76 to 11.64 ± 0.51 t/ha in 2016/2017. Selenate in comparison with selenite influenced the selenium accumulation in wheat grains more positively. However, a significant difference was recorded in variants with selenite application in the flag leaf growth phase in comparison with the end of tillering phase. The influence on the content of macroelements P, K, Ca and microelements Cu and Fe was observed in sodium selenite only; its application decreased the element content in comparison with the control variant. Statistically significantly higher values of fiber and fat were achieved after application of selenium in the flag leaf growth stage in comparison with the end of tillering.

Drought stress is a major environmental factor limiting crop growth and productivity. Hydrogen peroxide (H₂O₂) plays an essential role during stress response by acting as a signal molecule that activates multiple stress tolerance mechanisms. In this study, the effects of H₂O₂ on agronomic traits were studied in rice (Oryza sativa L.) cv. Khao Dawk Mali 105 (KDML 105) was subjected to drought stress. H₂O₂ was applied by either seed priming or foliar application method with a concentration of 1, 5, and 15 mmol/L. The results showed that both seed priming and foliar application with H₂O₂ improved some yield components. The tiller numbers, number of panicles, number of filled grains, filled grain weight, and harvest index were improved approximately 1.13, 1.04, 1.23, 1.21, and 1.1 times compared to the untreated plants. Foliar application, however, helps the plant by reducing yield loss as indicated by a 0.5-time reduction in the number of unfilled grain and lower unfilled grain weight. It was suggested that 5 mmol/L H₂O₂ was the most effective concentration to alleviate the effect of drought stress during the reproductive stage in rice.

Finding an appropriate method with the highest rate of polycyclic aromatic hydrocarbon (PAH) removal from naturally polluted soils is an important research issue. A pot factorial experiment (using contaminated soil samples from the Isfahan Refinery, Iran) was conducted in a 90-day period to compare the following bioremediation strategies: (1) natural attenuation (NA): the inherent ability of soil for bioremediation; (2) bioaugmentation (BA): inoculating soil with PAH degrading microbes Marinobacter hydrocarbonoclasticus; (3) biostimulation (BS): using N, P and K nutrients for the stimulation of bioremediating soil bacteria to achieve the C:N:P ratio of 100:10:1, and(4) bioaugmentation + biostimulation (BS + BA). Treatments NA (22.8%) and BA + BS (63.9%) resulted in the least and the highest rate of PAH removal from the soil. The 2-4 ring compounds had a significantly (P ≤ 0.05) higher rate of degradation than the 5-6 ring compounds. The highest rates were resulted by fluorene (76.41%) and acenaphthylene (72.28%) using the BA + BS treatment. However, the lowest degradation rates were resulted by indeno (1,2,3-cd) pyrene (10.05%), benzo [b] fluoranthene (10.17%), benzo (g, h, i) perylene (12.53%), and benzo [k] fluoranthene (13.67%), using NA treatment. The BA + BS treatments are the most effective method for the bioremediation of PAH polluted soils.

Intercropping system plays a crucial role in improving crop yield, nitrogen utilisation efficiency (NUE) and economic benefit. The difference in crop yield and interspecific relationship under different bandwidth and row ratio allocation patterns are still unclear. A field experiment was carried out to explore change regularities between crop yield and interspecific relationships under maize soybean intercropping with different bandwidths and row ratios. The results showed that the yield of intercropped crops was lower than that of the sole crop. The nitrogen accumulation (NA), NUE and nitrogen competition ratio was the highest under the intercropping mode with a bandwidth of 2.0 m, which indicated that this mode was more conducive to the N uptake and utilisation in crops. In all intercropping systems, nitrogen equivalent ratio (NER) and land equivalent ratio (LER) were all greater than one, indicating that intercropping systems were conducive to improving land utilisation efficiency and NUE. Under the same bandwidth pattern, expanding the maize soybean row ratio from 2:4 to 3:4 was beneficial to the improvement of LER, NER, NUE, crop group yield. In conclusion, it was preferable in the NA, NUE, crop group yield under the system of bandwidth 2.0 m and row ratio 2:2, which could be a reference for maize soybean intercropping system.

CO2 emissions represent one of the greenhouse gases significantly affecting climate change. Reduced tillage practices can contribute to the mitigation of CO2 emissions from soils. The effect of tillage practices with different straw incorporation on CO2 emissions was studied in field experiments in the years 2020 and 2021. The winter wheat straw was used in 2020, and spring barley straw in 2021. Treatments were: (1) chiselling to 10-12 cm; (2) shallow chiselling (5-6 cm depth); (3) straw mulch, and (4) stubble. The chiselling to 10-12 cm in the warm summer period of 2020 increased the CO2 emissions from soils even twice in comparison with other used soil tillage practices. The soil temperature and CO2 emissions decreased in the following order: chiselling to 10-12 cm < shallow chiselling ≤ stubble ≤ mulch in 2020. Low CO2 emissions without significant differences among treatments were observed in the year 2021 when low soil temperatures and excessive soil water content filling soil pores caused by intensive precipitations (23 mm) coming shortly after soil tillage were observed. The CO2 emissions from soils are affected by a number of factors. Among them the current weather conditions as well as soil temperature the depth of soil tillage and handling of postharvest residues, are important.

Present experiments were conducted to determine the effects of different irrigation levels on yield, yield components and quality of drip-irrigated sugar beet under sub-humid conditions. Field experiments were conducted in the 2019 and 2021 growing seasons in the Bursa province of Turkey. Experiments were carried out in completely randomised blocks design with three replications. Irrigations were scheduled based on the replenishment of 100 (S1), 66 (S2), 33 (S3), and 0% (S4) of soil water depletion within the soil profile of 90 cm using 7-day irrigation intervals. In 2019, root yields varied between 29.32 (S4)-86.31 (S1) t/ha and sugar yields between 6.33-13.57 t/ha. In 2021, root yields varied between 26.18 (S4)-74.56 (S1) t/ha and sugar yields between 6.56-12.53 t/ha. Effects of different irrigation levels on investigated parameters were found to be significant (P < 0.01). The crop water consumption values were significantly related to root and sugar yields (P < 0.01). Based on present findings, S1 treatment was recommended to get the highest root and sugar yields. In case of limited water resources, S2 (33% water shortage) treatment with the highest water productivity and irrigation water productivity values could be recommended to ensure maximum efficiency from the applied irrigation water quantity.

The study determined the effects of the speed of loading and the loading orientation on some selected mechanical properties of the TME 419 cassava tuber variety at different ages of the tuber which are essential in the design and construction of the processing and handling equipment of a cassava peeler. The properties considered include the bioyield and rupture points, compressive and rupture strengths, toughness and firmness, and moduli of stiffness and toughness, which were carried out in the transverse and longitudinal loading direction using an Instron Universal Testing Machine (UTM). As the loading rate increased from 5.00 to10.00 mm.min^-1 and the age of the tuber varies from 1.00 to 2.00 years, the bioyield and rupture points, compressive and rupture strengths, toughness, firmness, moduli of stiffness and toughness in the transverse and longitudinal direction varies from 1 619.61 to 3 636.19 N and 136.08 to 384.52 N, 0.48066 to 1.07913 N.mm^-2 and 0.26604 to 0.75173 N.mm^-2, 766 to 1055 N.mm^-1 and 1 262 to 2 965 N.mm^-1, 303.98 to 553.68 mm.min^-1 and 28.08 to 53.71 mm.min^-1 2.30 to 4.19 N.mm^-2 and 5.376 to 8.94N.mm^-2 respectively. Generally, the values of the properties examined are higher in the longitudinal loading orientation than in the transverse and for a year and half old tuber which will be useful in designing an efficient cassava peeling system.

Soil properties can be influenced by long-term agricultural management practices as described in pedological literature. In this study, selected physical properties (particle density and bulk density, total porosity, maximum capillary water capacity, minimum air capacity, field capacity, permanent wilting point and available water capacity) of topsoils from different reference soil groups (Cambisols, Luvisols, Fluvisols, Chernozems and Phaeozems, Leptosols, Stagnosols and Gleysols) were sampled and analysed in the years 2016-2017. The topsoil samples were taken from points of so-called S (specific) soil pits to be sampled from the General Soil Survey of Agricultural Soils (GSSAS) which was accomplished in the years 1961-1970. In addition, some of the properties were also compared with those measured during the GSSAS. Recognising the properties, only the particle density, the maximum capillary water capacity, the permanent wilting point and the available water capacity of the topsoil of the individual soil groups were statistically significantly (P < 0.05) different. A comparison of the physical properties with those analysed after more than 40 years was performed, the bulk density increased and the total porosity decreased in the topsoil of the major part of the studied soil groups.

Freeze-thaw erosion occurs primarily at high latitudes and altitudes. Temperature controlled freeze-thaw events dislodge soil particles and serve as a catalyst for erosion. This review paper provided an overview of the effects of freeze-thaw on soil properties and water erosion. The process of freeze-thaw cycles results in temporary and inconsistent changes in the soil moisture, and affects the soil's mechanical, physical and chemical properties, such as the soil moisture content, porosity, bulk density, aggregates stability, shear strength and organic matter content and so on. The variation trend and range of the soil properties were related to the soil texture, water content and freeze-thaw degree. Furthermore, the soil erosion was affected by the freeze-thaw processes, as thawing and water erosion reinforce each other. However, research of different experimental conditions on indoor simulations have numerous limitations compared with field experiments. The use of indoor and field experiments to further reveal the freeze-thaw effect on the soil erosion would facilitate improved forecasting.

Forest soils have a high potential to store carbon and thus mitigate climate change. The information on spatial distribution of soil organic carbon (SOC) stocks is thus very important. This study aims to analyse the importance of environmental predictors for forest SOC stock prediction at the regional and national scale in the Czech Republic. A big database of forest soil data for more than 7 000 sites was compiled from several surveys. SOC stocks were calculated from SOC content and bulk density for the topsoil mineral layer 0-30 cm. Spatial prediction models were developed separately for individual natural forest areas and for four subsets with different altitude range, using random forest method. The importance of environmental predictors in the models strongly differs between regions and altitudes. At lower altitudes, forest edaphic series and soil classes are strong predictors, while at higher altitudes the predictors related to topography become more important. The importance of soil classes depends on the pedodiversity level and on the difference in SOC stock between the classes. The contribution of forest types as predictors is limited when one (mostly coniferous) type dominates. Better prediction results can be obtained in smaller, but consistent regions, like some natural forest areas.

Dog barking, which reaches around 100 dB, often becomes a noise issue in urban environments. One potential solution, a simple soundproof cage, has recently been marketed. To our knowledge, no study has been conducted to investigate what sort of stress burden is imposed on dogs kept in cages, and it may raise animal welfare concerns. The purpose of this study was, therefore, to reveal whether staying in a soundproof cage caused stress for the dogs or not. Ten healthy domestic dogs (5 males, 5 females) of small body size were evaluated. The heart rate variability (HRV), behaviour, and internal and external temperature during confinement in a soundproof cage for each dog were analysed. The HRV analyses revealed no significant differences for any variables between confinement and non-confinement (HR, P > 0.999 9; rMSSD, P = 0.359 4; SDNN, P = 0.359 4; LF, P = 0.652 3; HF, P = 0.128 9; LF/HF, P = 0.222 7). Overall, in our behavioural analysis, there were no significant differences between confinement and non-confinement (P = 0.105 5). In conclusion, the HRV and behavioural analyses did not indicate an increased stress burden on the dogs during confinement in the soundproof cage compared with non-confinement. The inner temperature of the cage was not elevated either. Nevertheless, the stress imposed on dogs caused by a soundproof cage should always be considered on an individual basis, and the time spent in such a cage should always be kept to a minimum.

In human medicine, skin grafting is an innovative surgical technique widely used in reconstructive surgery to repair skin loss. This case evaluated the effectiveness of a treatment with dermal micro-grafting obtained through the Rigenera^® technology in a chronic open wound resulting from a suture dehiscence of a limb amputation in a cat. Significant differences were observed between the aesthetic aspects of the injury using traditional treatments (cleaning and curettage) and the regenerative technology. The results showed that the healing periods were significantly reduced after the Rigenera^® treatment and that, moreover, a perfect skin status and a complete reduction in the wound area (100%) were achieved in one month. Given these results, Rigenera^® has proven to be a simple yet highly effective method in the treatment of inactivated chronic wounds.

The aim of this clinical retrospective study was to analyse the prevalence of Trichomonas gallinae in budgerigars (Melopsittacus undulatus). The role of T. gallinae in budgerigars as the cause of a typical clinical picture (young vomiting budgerigars) is clear; however, the frequency of latent and subclinical diseases in pet birds is unclear. Over the period of 20 years (2000-2019), 16 759 birds were examined, of which 5 256 (31.36%) belonged to the genus Melopsittacus (budgerigars). Within the 5 256 presented budgerigars, 2 547 (48.45%) were examined for trichomonads using a microscopic examination of a crop wash, and, of those, 978 birds (38.39%) were found to be positive for T. gallinae. Only 96 (9.8%) of the positive budgerigars showed typical signs of the disease, such as loss of appetite, vomiting, weight loss or anorexia. Unspecific clinical signs were seen in 312 (31.9%), while 570 birds (58.3%) did not show any clinical signs and had been presented for a routine check or other medical problems. Treatment using metronidazole (Anaerobex, Flagyl) was successful in 82-85% of these diseased birds. Trichomonads can be detected in over a third of budgerigars, even if less than 10% of the positive ones show clinical symptoms.

The effect of Bacillus subtilis on the immune responses and morphometry of the immune organs was evaluated in broilers challenged with S. gallinarum. For this purpose, Salmonella-free birds (n = 240) were split into four groups with six replicates of ten birds each. Groups included an NC (negative control, non-infected + non-medicated), a PC-S (positive control, Salmonella-infected + non-medicated), an AT-S (Salmonella-infected + medicated with enrofloxacin), and a BS-S (Salmonella-infected + B. subtilis (2.0 × 10¹⁰ cfu/g; 0.1 g/kg) group. On day 21, the thickness of the thymus cortex and medulla, germinal centre area of the spleen, bursal follicular length and bursal follicular area increased (P < 0.05) in the BS-S when compared to the NC and PC-S groups. On day 35, the BS-S group exhibited a higher (P < 0.05) antibody titre against the Newcastle disease virus (NDV), and cortex of the thymus was thicker (P < 0.05) compared to the other groups. A decrease in the thymus medulla thickness, germinal area of the spleen and bursal follicular number were noted in the PC-S group when compared to the other treatment groups. In conclusion, the prophylactic use of B. subtilis type probiotics alleviated the stress resulting from a Salmonella gallinarum infection and improved the immune organs development and function in infected broilers.

This study explored the regulatory effect of temperature stress on fatty acid biosynthesis in the early stage of common carp (Cyprinus carpio) based on Δ6-fatty acids desaturase (Δ6FAD) and elongase-5 (ELOVL5) gene expression and fatty acid composition. One-day-old carp larvae were selected, and after seven days of acclimatisation at 25 °C, the larvae were subjected to temperature stress for 96 h in water at 32 °C or 10 °C. In the post-larval stage, 30-day-old carp juveniles were selected and, after seven days of acclimatisation at 25 °C, were subjected to temperature stress for seven days in water at 32 °C or 10 °C. The results showed that common carp larvae could rapidly and highly express Δ6FAD and ELOVL5 genes within 48 h at high temperature (32 °C) compared with the 0 h group (P < 0.05), while gene expression began to gradually increase after 48 h at low temperature (10 °C). There was a significant improvement in C22:6n-3 and C20:5n-3 after 96 h at low temperature compared with the 0 h group (P < 0.05). In common carp juveniles, the Δ6FAD gene in the intestine, brain and liver was sensitive to low temperature, but the ELOVL5 gene in the intestine, brain and liver of common carp was sensitive to high temperature. The low temperature increased the amount of highly unsaturated fatty acids (HUFA) in the common carp juveniles. The results indicated that temperature could regulate the expression of the Δ6FAD and ELOVL5 genes for HUFA production, as well as for participation in the biosynthesis of fatty acids in the body during the early development of common carp. The results of this study help clarify the regulatory effects of temperature on fatty acid biosynthesis during the early development of common carp.

Technology has made it possible to achieve the very efficient use of water resources in agriculture. However, there are a set of practices that could increase both the availability and quality of the water resources, but which are not yet widely used. In view of the scarcity situation mainly produced by the consequences of climate change, the objective of this work is to analyse the adoption of sustainable irrigation management practices in agriculture. To this end, the aim is to identify and evaluate the variables involved in adopting sustainable practices in agriculture through the use of different qualitative research tools in successive phases (literature review, in-depth interviews, Delphi method and workshop). The results indicate that the sustainable practices to be adopted to improve water management in the study area are rainwater harvesting (RWH) and pond covering (PC). The main barriers are the costs, some farm characteristics and lack of research, while the facilitators include easy access to technology and the existence of farmer-to-farmer networks. Furthermore, the most influential stakeholders for these practices are the farmers, policymakers and researchers. Proposals for the adoption of sustainable water use practices have also been made consensually with all the involved agents.