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Will cultural and regulatory forest services provide enough balance to provisioning services in forest enterprise portfolios in future? Marketing case studies of selected forest enterprises in SlovakiaOriginal Paper

Alex Bumbera, Daniel Halaj, Iveta Hajdúchová

J. For. Sci., 2025, 71(2):59-71 | DOI: 10.17221/68/2024-JFS

The study aims to identify the difference between the current and future strategic management of forest ecosystem services (FES) portfolios in public enterprises of Slovakia. The case studies focus on forest enterprises in Ko¹ice, Banská ©tiavnica, Kremnica, and Bratislava, representing the best practice examples of providing cultural services in the country. A marketing decision-making model, Boston Consulting Group (BCG) matrix, was used to analyse each company's current FES portfolio. Content analysis assessed the data collected from interviews with managers of the public forest enterprises. Duncker's forest management approaches classification was applied to evaluate the future strategic management of the FES portfolio, and a horizon of 10 years was set. According to the results, the urban forest enterprise in Bratislava is an example of receiving a subsidy from the capital for building and reconstructing recreational facilities. Therefore, this FES portfolio is and will be the most balanced. The portfolio of the forest enterprises in Ko¹ice, Kremnica, and Banská ©tiavnica will remain unchanged for the next decade, and they plan to use a combination management approach that caters to economic, ecological, and social needs and objectives. Urban forests in Bratislava will focus even more on close-to-nature forestry.

List of reviewers 2024List of reviewers

Editorial Department

J. For. Sci., 2025, 71(1):I-II

List of reviewers 2024

Editorial Department

Agric. Econ. - Czech, 2025, 71(1):I-III

Bridging credit gaps for sustainable agriculture: The role of rural savings and credit cooperatives among smallholder farmersOriginal Paper

Gershom Endelani Mwalupaso, Shangao Wang, Xianhui Geng, Shadrack Kipkogei

Agric. Econ. - Czech, 2025, 71(5):254-272 | DOI: 10.17221/436/2024-AGRICECON

Despite the recognised benefits of climate smart agriculture (CSA) in enhancing farmers' adaptive capacity to climate risks, adoption rates remain low in Sub-Saharan Africa. This disparity can be attributed, in part, to the significant challenges smallholder farmers face in accessing credit from the formal financial sector. In response, Rural Saving and Credit Cooperatives (RUSACCOs) have emerged as crucial sources of funding for both household expenses and agricultural activities. However, despite their increasing importance in improving financial inclusion, little is known about whether participation in RUSACCOs can help alleviate existing credit constraints and promote the adoption of CSA among smallholder farmers. To address this knowledge gap, we employ a recursive bivariate probit (RBP) and propensity score matching (PSM) analysis using data from 400 randomly selected smallholder farmers in Zambia. The analysis controls for three main sources of endogeneity: program placement, endogenous covariates, and self-selection. Our findings indicate that participation in RUSACCOs has the potential to mitigate farmers' credit constraints by 42% and facilitate CSA adoption by 25%. Notably, the alleviation of existing credit constraints is associated with a 14% increase in CSA adoption. These results underscore the previously overlooked role of RUSACCOs in promoting agricultural sustainability. By effectively addressing financial inclusion barriers and providing access to practical agricultural knowledge, RUSACCOs can contribute to reducing the vulnerability of agriculture while fostering sustainable production. Our study suggests that repurposing RUSACCOs to emphasise financial inclusion and promote access to agricultural learning platforms can yield triple benefits: agricultural, environmental, and livelihood sustainability.

Revealing the challenges and prospects of Asian rice gall midge resistance in riceReview

Sivasubramaniyan Sathishkumar, Loganathan Arul, Swaminathan Manonmani, Sheela Venugopal, Jagadeesan Ramalingam

Plant Protect. Sci., 2025, 61(4):305-325 | DOI: 10.17221/159/2024-PPS


The Asian rice gall midge (Orseolia oryzae) (Wood-Mason) is a major pest of rice, significantly reducing yields and challenging sustainable rice production. This review provides a comprehensive overview of the biology, lifecycle and geographical distribution of the rice gall midge, along with the damage symptoms it causes in rice crops. The interactions between rice and gall midge, the pest's infestation mechanism and the plant’s defensive responses are also explored. Various management strategies are discussed in detail, including insecticides, cultural practices and resistant varieties. The review emphasises that breeding for resistance, especially through the pyramiding of resistance genes and integrated pest management approaches, shows the most promise for long-term control. Advances in crop improvement through breeding methods such as genotyping, phenotyping, field and greenhouse screening and the pyramiding of resistance genes are highlighted. The review emphasises the importance of monitoring virulence in gall midge populations to guide breeding efforts. The genetic basis of resistance is examined through studies of resistance genes, QTL mapping and marker-assisted breeding. Furthermore, molecular approaches, including metabolomic regulations, microarray analysis and biotechnological strategies, are reviewed for their potential in developing durable gall midge-resistant rice varieties. This article synthesises the current knowledge and highlights future research directions, such as identifying novel resistance genes, improving molecular breeding techniques, and developing integrated pest management strategies that combine genetic resistance with eco-friendly controls.

Role of glycine betaine in mitigating salt-induced oxidative stress in Vigna radiataOriginal Paper

Khalid H. Alamer

Plant Soil Environ., 2026, 72(1):1-15 | DOI: 10.17221/451/2025-PSE

The impact of exogenously applied glycine betaine (GB; 0, 5, 10, 20 and 50 mmol) was evaluated in preventing Vigna radiata from the adverse effects of salt (100 mmol NaCl) stress. Salinity reduced growth parameters, such as plant height and fresh and dry weight of plants, while GB application significantly alleviated the decline. Salinity stress led to a decline in total chlorophylls and carotenoids, as well as a reduction in the net photosynthetic rate and gas exchange attributes, including stomatal conductance, transpiration rate, and intercellular CO2. However, GB supplementation significantly alleviated this decline. Salinity stress increased the accumulation of hydrogen peroxide, superoxide and methylglyoxal, while as applied GB reduced their accumulation, causing a significant decline in the lipid peroxidation. Application of GB, at all concentrations, increased the activity of the antioxidant enzymes under normal and salinity stress treatments with 10 and 20 mmol concentrations, imparting the highest increase. Increase in the radical scavenging activity due to GB application was also supported by increased total antioxidant activity assays measured as percent DPPH and ABTS radical scavenging. In addition, GB-supplemented plants exhibited an apparent increase in the activities of glyoxalase I and glyoxalase II enzymes. Accumulation of osmotic compounds like proline, sugars and GB increased significantly due to GB application and showed a further increase in salt-stressed plants. More importantly, the GB-treated plants exhibited a considerable decline in sodium accumulation, causing a decline Na/K in them. Glycine betaine was effective in mitigating the deleterious effects of salinity.

List of Reviewers 2024List of reviewers

Editorial Office

Hort. Sci. (Prague), 2025, 52(1)

List of varieties registered in the Czech Republic in 2024New Varieties

Tomá¹ Mezlík

Czech J. Genet. Plant Breed., 2025, 61(2):110-112 | DOI: 10.17221/16/2025-CJGPB

The role of seed transmission in the spread of cereal viruses: Global challenges and prevalent threats in UkraineReview

Halyna Snihur, Tetiana Shevchenko, Oleksiy Shevchenko, Anhelina Kyrychenko

Plant Protect. Sci., 2025, 61(3):201-221 | DOI: 10.17221/51/2025-PPS

The transmission of plant viruses through seed plays a fundamental role in virus spread, persistence, and survival, particularly in economically important crops. Besides its considerable ecological significance, seed transmission influences plant and virus evolution. Virus contamination of the seed also has critical epidemiological implications, especially when combined with subsequent or additional insect vector spread. Plants grown from contaminated seeds serve as primary viral inoculum sources, facilitating the introduction of viruses into new regions and triggering disease outbreaks with substantial economic losses for growers. Changes in environmental conditions increasingly influence plant virus epidemiology by affecting vector populations, host susceptibility, and transmission dynamics, thus increasing virus transmission risks in cereal crops. This review explores the mechanisms of seed transmission and its consequences, with a focus on key cereal viruses in Ukraine: barley stripe mosaic virus, wheat streak mosaic virus, High Plains wheat mosaic virus, sugarcane mosaic virus, and maize dwarf mosaic virus. Hereby, the biological properties of these viruses, the risks posed by seed transmission, and the economic impact on crop production are discussed. Given the widespread distribution of these pathogens, presented data will also be valuable for other cereal-growing regions, particularly those bordering Ukraine and engaged in seed import/export. This review underscores the global need to manage seed-transmitted viruses to safeguard cereal crop productivity and food security. Future research should focus on developing resistant cultivars and advanced diagnostics to control their spread.

Flavonoids as bio-insecticides: Harnessing plant metabolites as a biochemical shield against insectsReview

Thamaraikannan Sivakumar, Sunilkumar Devanathan, Parthasarathi Ganesan, Kavithamani Duraisamy, Murugan Marimuthu, Vellaikumar Sampathrajan, Karthikeyan Adhimoolam, Senthil Natesan (ORCI

Plant Protect. Sci., 2026, 62(3):191-209 | DOI: 10.17221/56/2025-PPS

The global decline in crop production poses a significant threat to food security, particularly in the context of a growing human population. Among various environmental constraints on agriculture, biotic stress, particularly that caused by insect pests, remains a major reason for yield losses. Traditionally, synthetic pesticides have been used to manage insect infestations; however, their excessive and non-targeted application has raised serious concerns regarding environmental pollution, adverse health effects, and the accelerated development of pesticide-resistant pest populations. In this context, plant-derived biocactive compounds, particularly flavonoids, have emerged as promising bioinsecticides due to their potent insecticidal properties. Flavonoids, a diverse group of secondary metabolites found abundantly in plants, exhibit strong insecticidal activity by disrupting insect digestion, interfering with nutrient absorption, and inhibiting growth and metamorphosis. These bioactive compounds act through multiple mechanisms, reducing the likelihood of resistance development while offering an eco-friendly alternative to conventional chemical pesticides. Additionally, flavonoids contribute to integrated pest management strategies by enhancing plant defence responses and synergising with other bioinsecticides. Despite their potential, research on flavonoid-based insect control remains limited, particularly in terms of their formulation, stability, and large-scale applicability. Further studies are needed to investigate their interactions with insect physiology, optimise delivery methods, and assess their environmental impact. Advancing flavonoid-based bioinsecticides can contribute significantly to sustainable pest management in modern agriculture, reducing dependence on synthetic pesticides while preserving ecosystem balance. This review examines the potential role of flavonoids as biopesticides in pest management, highlighting the existing research gaps and prospects.

Consumer perceptions of lab-grown cells: Awareness, barriers, and the power of information. A reviewReview

Katalin Szendrõ

Czech J. Anim. Sci., 2025, 70(6):203-222 | DOI: 10.17221/31/2025-CJAS


Lab-grown cells (also known as cell-based or cultured meat) are novel food innovations that face uncertain consumer acceptance. This review examines recent research (2020–2024) on consumer perceptions of lab-grown cells, focusing on three main areas: (1) public awareness and familiarity, (2) psychological and cultural aspects shaping perceptions, and (3) the impact of information on acceptance. Results show that the awareness of lab-grown cells varies widely across regions; in countries with high meat consumption, such as the USA and Australia, or where culinary delights are highly valued, such as France, respondents rated lab-grown cells lower than in those countries where meat consumption is now rising, for example, China or Mexico. The knowledge of lab-grown cells positively impacted attitude, while psychological barriers (e.g. food neophobia) and disgust emerged as key deterrents. The information provided to respondents significantly influenced their willingness to accept, buy, try, eat, and pay premium prices. Personal (mainly health-related) benefits significantly increased the acceptance of lab-grown cells, while societal benefits (e.g. benefits to the environment or animal welfare) had less prominent effects than expected. At the same time, information regarding production technology (high-tech, laboratory, artificial) resulted in lower ratings from respondents. The conclusion is that overlooking the positive attributes of meat and focusing on the unproven advantages of lab-grown cells may lead to misleading results. On the other hand, effective communication – especially messages highlighting personal benefits – can substantially improve the consumer openness.

Toxicity of oxalic acid and its toxic effect on antioxidative enzymes in honey bee larvaeOriginal Paper

T Majchrak, M Ratvaj, L Sabova, J Toporcak, L Molnar

Vet Med - Czech, 2025, 70(7):261-271 | DOI: 10.17221/18/2025-VETMED

The production of food of plant origin is critically dependent on the pollination ability of honey bees, whose health has been deteriorating for a long time, and whose population is declining. In our in vivo experiment on a honey bee brood at the 4-day larval stage, we tested the following concentrations of oxalic acid: 0% (control – applied distilled water), 0.87%, 1.75%, 3.5% and 7%, corresponding to doses of 0 mg, 2.61 mg, 5.25 mg, 10.5 mg, and 21 mg of oxalic acid per dm2 of honeycomb with the brood. The LC50 values (72 h) ranged between 3.17% and 3.33%. The different LC50 values obtained resulted from three different methods used to calculate this indicator. The therapeutic index (TI) of oxalic acid was set to be 1.1, indicating a high risk to the honey bee brood. We observed an increased gene expression for the detoxifying enzyme glutathione-S-transferase (GST), but did not detect an increased gene expression for superoxide dismutase (SOD1 and SOD2), which protects the organism from oxidative stress. A decrease in gene expression was observed for prophenoloxidase and hymenoptaecin, while defensin and lysozyme did not show significant changes. These results emphasise the need for the accurate dosage and application of oxalic acid in the treatment of varroosis.

Key features for forest bathing development: A Q-methodology study applied in ItalyOriginal Paper

Sofia Baldessari, Alessandro Paletto, Giorgia Di Domenico, Valerio Di Stefano, Sandro Sacchelli

J. For. Sci., 2025, 71(7):347-357 | DOI: 10.17221/37/2025-JFS

Forest bathing is gaining attention for its health and well-being benefits, leading to growing interest among academics, policymakers, and practitioners. While most studies have focused on its physiological and psychological effects, less is known about how different stakeholders perceive the characteristics that make forest bathing effective and meaningful. This study explored expert and non-expert perspectives on forest bathing in Italy through the Q-methodology, aiming to identify shared and divergent views on key aspects such as site features, accessibility, and perceived benefits. The results highlighted four distinct viewpoints: a scientific-health-oriented perspective emphasising medical efficacy (mainly experts); a preference for more naturalness and ecological integrity (mainly non-experts); a possible socio-economic opportunity (mainly non-experts); and a preference for a more managed forest environment with supporting structures (mainly experts). Despite differences, consensus emerged on several points, including the cultural and physical relevance of forest bathing and the importance of inclusive accessibility while preserving low-impact environments. The findings highlighted the potential of Q-methodology to capture the plurality of stakeholder voices, offering a basis for more inclusive planning and policy development in the context of nature-based well-being initiatives.

Wheat (C3) and maize (C4) adaptive responses to soil thallium toxicity under elevated CO2 conditionsOriginal Paper

Wael A. Obaid, Samy Selim, Seham M. Hamed, Emad A. Alsherif, Shereen Magdy Korany, Hana Sonbol, Danyah A. Aldailami, Soad K. Al Jaouni

Plant Soil Environ., 2025, 71(8):534-552 | DOI: 10.17221/239/2025-PSE

This study investigated how wheat (C3) and maize (C4) respond to soil thallium (Tl) contamination and elevated CO2 (eCO2), aiming to understand strategies for mitigating oxidative stress. Under eCO2, both crops showed higher biomass production. However, high Tl concentration (120 mg/kg) significantly decreased fresh and dry weights by 31–59%, which translated directly to compromised yield. This growth decline is linked to impaired photosynthesis, evidenced by a 54–57% drop in net photosynthetic rate under elevated Tl. Such photosynthetic inhibition intensifies oxidative stress, marked by increased membrane damage and hydrogen peroxide (H2O2). Furthermore, photorespiration contributed to oxidative stress by generating H2O2, with increased activities of glycolate oxidase and hydroxypyruvate reductase rising by 122% and 201%, in wheat and by 179% and 39% in maize, respectively, in response to 120 mg/kg TI under eCO2 conditions. Simultaneously, to mitigate oxidative damage, antioxidant defences were significantly enhanced, resulting in increased activity of the ascorbate (ASC)/glutathione (GSH) cycle, along with elevated levels of metallothionein and phytochelatin for Tl sequestration, as well as augmented glutathione S-transferase activity. Overall, findings reveal complex interactions between CO2 and Tl, highlighting species-specific adaptive responses of C3 and C4 plants. C3 plants use photorespiration to combat oxidative stress, while C3 and C4 plants have strong antioxidant systems to reduce the effects of oxidative stress, promoting crop resilience and growth despite Tl toxicity.

Soil macroarthropod dynamics in response to environmental disturbances in a forest remnant ecosystem: A case study at Cibodas Botanical GardenOriginal Paper

Anita Rianti, Fenky Marsandi, Taufikurrahman Nasution, Musyarofah Zuhri, Muhammad Efendi, Hari Prayogi, Setyawan Agung Danarto, Hidayatul Fajri, Vivin Silvaliandra Sihombing (ORC

J. For. Sci., 2026, 72(1):1-13 | DOI: 10.17221/38/2025-JFS


Disturbing the remaining forest ecosystem in the Cibodas Botanical Garden (CBG) has affected the dynamics of the soil macroarthropod communities. This study was conducted in three remaining forest locations in the CBG with different levels of disturbance. Soil macroarthropod samples were collected using the pitfall trap method with 30 traps and analysed using the Shannon-Wiener diversity index, Pielou's evenness, Simpson's dominance, and Margalef's species richness to assess the dynamics of the soil macroarthropod community. This study analysed how these communities respond to different levels of disturbance in the garden, namely Jalan Akar (JA; low), Wornojiwo (WJ; moderate), and Ciismun (CI; high), which were influenced by tourism activities and local environmental conditions. The results showed that individuals from the Hymenoptera group accounted for 60.05% of the total number of soil macroarthropods found. Site WJ, which experienced moderate disturbance, had the highest number of individuals and species richness of soil macroarthropods. In contrast, site CI, which experienced high levels of disturbance, had a lower number of individuals and lower species richness, diversity and evenness indices. Site JA, which experienced low levels of disturbance, exhibited higher diversity and evenness indices. These results demonstrate that disturbance affects the presence of soil macroarthropods at their respective levels of disturbance. However, analysing the spatial distribution of soil macroarthropods in each studied taxon using the Morisita index revealed that they were dominantly clustered and exhibited varied distribution patterns. The study concludes that maintaining minimal disturbance is essential to preserve soil biodiversity and ecological balance in managed forest ecosystems such as the Cibodas Botanical Garden.

Assessment of genetic diversity and population structure of almond germplasm in Van Province, Türkiye, using iPBS-retrotransposon-based markersOriginal Paper

Berna Sarman, Metin Koçak

Czech J. Genet. Plant Breed., 2026, 62(3):135-145 | DOI: 10.17221/12/2026-CJGPB

The genetic characterisation of naturally occurring almond genotypes can guide the selection of genetic resources to be used in the breeding programme. Therefore, this study aims to assess the genetic diversity and population structure of almond germplasm comprising 50 accessions naturally occurring in Van, Türkiye, along with two commercial varieties (Pabuç and Dokuzoğuz). Thirteen inter-primer binding site (iPBS) retrotransposon markers generated a total of 102 bands, of which 95 were polymorphic. The average polymorphic band number per marker was 7.3, with a range of 5 to 13. A formula yielding a maximum of 0.5 resulted in polymorphic information content (PIC) values between 0.27 and 0.43, with a mean value of 0.36. Unweighted pair group method algorithm (UPGMA), principal coordinate analysis (PCoA), and STRUCTURE analysis, based on Bayesian clustering analysis, yielded consistent results, indicating that local populations (Akdamar and Çarpanak) were distinctly grouped, while commercial accessions were clustered with Çarpanak accessions. The diversity metrics and classification analysis utilising 13 iPBS-retrotransposon markers demonstrated that the iPBS-retrotransposon marker system possesses significant promise for evaluating the genetic variety and population structure of almonds.

Beta-hydroxy-beta-methylbutyrate in functional food ingredients: An overview of biosynthesis, metabolic mechanisms and applicationsReview

Xizi Zhang, Huijing Zhang, Jiaxing Li, Chenchen Qi, Di Zhang, Wei Chen, Chengtao Wang

Czech J. Food Sci., 2026, 44(1):1-15 | DOI: 10.17221/88/2025-CJFS

β-Hydroxy-β-methylbutyrate (HMB) is a metabolite of the essential amino acid leucine, which can be produced naturally in mammals and is also found in trace amounts in citrus fruits and fish. Studies have shown that HMB plays an important role in maintaining human health by improving muscle health and inhibiting muscle catabolism. This review summarises the synthesis and metabolism of HMB and discusses its potential use as a nutrient, highlighting and analysing the importance of HMB supplementation for athletes' physical recovery and the treatment of muscular dystrophy-related diseases between 2019 and 2025. This study will help us to deepen our understanding of the application of HMB as a dietary supplement for the treatment of different diseases, providing the latest insights into its sustainability.

Discovering complex pesticide pollution in river water irrigated soil/groundwater systems: From targeted analyses to non-targeted screening and backOriginal Paper

Alina Sadchenko, Petra Nováková, Ale¹ Klement, Miroslav Fér, Antonín Nikodem, Vít Kode¹, Radka Kode¹ová, Roman Grabic

[Ahead of Print]CAAS Agricultural Journals, X:X | DOI: 10.17221/30/2026-SWR

Pesticides and their transformation products are increasingly detected in agricultural soils and surface waters, raising concerns about their persistence, mobility, and ecological impacts. Irrigation with river water contaminated by agricultural runoff represents a significant but understudied pathway contributing to soil pollution. In this study, we investigated pesticide occurrence across soils, irrigation water, and groundwater in three intensively cultivated river basins. Soil samples from vegetable-producing fields exhibited complex contamination profiles, with 12–40 co-occurring compounds and total residues frequently exceeding levels reported for European arable soils. Several pesticides, including pendimethalin, mandipropamid, and azoxystrobin, were found at notably high concentrations, with some soils surpassing 5 000 µg/kg. Comparison of detection frequencies across matrices revealed diverse transport and retention behaviour: while certain legacy compounds (e.g., atrazine metabolites) were ubiquitous in both surface and groundwater, others showed strong soil accumulation with limited mobility. Irrigation water was identified as a non-negligible contamination source, particularly for persistent and mobile substances, although direct field applications remained the dominant contributor to peak soil concentrations. By integrating targeted and non-target screening, this study provides the most comprehensive assessment to date of pesticide burdens in riverirrigated agricultural soils and highlights the need for improved monitoring strategies in systems where soil and water pollution are tightly interconnected.

Decoupling of stomatal and mesophyll recovery drives photosynthetic resilience to water deficit in sugar beet: evidence from multiscale structural and functional traitsOriginal Paper

Yangyang Li, Zengyuan Tian, Jixia Su, Kaiyong Wang, Pengpeng Zhang, Hua Fan

Plant Soil Environ., 2026, 72(1):49-65 | DOI: 10.17221/564/2025-PSE

Water deficit severely constrains sugar beet productivity by impairing photosynthetic capacity. However, the underlying structure-function mechanisms conferring photosynthetic resilience remain poorly characterised. This study investigates the temporal dynamics of photosynthetic limitations and structural adaptations in sugar beet during water deficit and subsequent rehydration. We found that water deficit significantly reduced the maximum net CO2 assimilation rate (ANmax) and the Rubisco carboxylation rate (Vcmax) by impairing CO2 diffusion and biochemical processes. The reduction in photosynthetic capacity is primarily and stably attributed to mesophyll limitation, while contributions from stomatal and biochemical limitations flexibly change with deficit degree and rehydration. Severe water deficit caused irreversible structural damage that hinders recovery even after rehydration, while moderate water deficit allows partial restoration of leaf and chloroplast function. Partial least squares structural equation modelling (PLS-SEM) demonstrated that CO2 diffusion was governed by the volume fraction of intercellular air space (fias, β = 0.28) and surface areas of the chloroplasts exposed to leaf intercellular air spaces (Sc/S, β = 0.35), with Sc/S indirectly influencing mesophyll conductance (gm) through fias mediation (β = 0.53). Severe water deficit caused irreversible fias reduction and chloroplast interface damage (59% cell volume loss). These findings establish that resilience to water deficit in sugar beet depends on mesophyll structural integrity, with fias and Sc/S as key modulators of gm recovery. The study advances understanding of stress recovery mechanisms in sugar beet and provides a framework for multiscale crop improvement in the context of climate change.

Coppice forests: Between management, conversion and restorationReview

Valeriu-Norocel Nicolescu, Gavriil Spyroglou, Sanja Periæ, Martina Ðodan, Cornelia Buzatu-Goanțã, Milun Krstic, Joao Carvalho, Iryna Matsiakh, Halil Bariº Özel (ORCID:

J. For. Sci., 2026, 72(2):57-72 | DOI: 10.17221/5/2026-JFS


Coppice is the oldest form of systematic and sustainable use of forests, and is currently applied on about 29 million ha (about 14% of total forest land area) in Europe. It had its maximum spread in the 16th century, when an estimated 36% of all forested land in Europe was managed for coppice. Coppice forests were the most important source of fuelwood until the mid-19th century, when firewood and charcoal were substituted by alternative fuels, and the demand for construction wood increased. Consequently, coppices (both low and coppice-with-standards) started to be converted to high forests, and the process was driven by national policies and/or subsidies, which is still the situation in some European countries. During the 20th century, coppicing was abandoned in many places across Europe due to the abandonment of the countryside and population migration into cities, as well as changes in socio-economic conditions, technical advances and political restrictions. However, coppicing is still important in many European countries as the main source of firewood for the rural population, who has limited access to other sources of energy. In this context, this paper presents the most important characteristics of this complex abandonment/management/conversion picture, emphasising the pros and cons for the future of coppices across Europe.

Effect of bacteriophages on growth performance and health indicators in broiler chickens in the absence of bacterial challenge – A reviewReview

MA Mohd Shaufi, SL Chia, H Abu Hassim, MT Yusof, MA Akbar, AAL Song, S Mustafa

Vet Med - Czech, 2026, 71(2):35-53 | DOI: 10.17221/5/2025-VETMED

This review examines bacteriophages as alternatives to antibiotic growth promoters (AGPs) in broiler chickens, with a unique focus on effects observed in studies without experimental bacterial challenges. Driven by global antimicrobial resistance (AMR) concerns and sustainable poultry production demands, phage supplementation, a targeted strategy, potentially improves growth performance and gut health by preserving beneficial microbiota. This literature analysis assesses phage efficacy in healthy broilers under non-challenge conditions, evaluating key variables such as phage concentration, delivery, and targets, as well as outcomes such as feed conversion ratio (FCR) and gut health markers. Crucially, this review extends beyond efficacy to explore phage immunomodulatory capabilities, outlines optimisation strategies, and addresses risks and mitigation. Key findings show mixed efficacy of phages in non-challenged broilers: significant improvements in FCR and gut health were generally observed with high phage concentrations (e.g., ≥108 PFU/g) and continuous delivery protocols, whereas lower doses yielded inconsistent or minimal benefits. Furthermore, choosing which bacteria phages target – for example, aiming at pathogens like Salmonella or managing common gut bacteria such as E. coli – greatly impacts outcomes. While phages show considerable potential as AGP alternatives, the review highlights that further research is vital to resolve inconsistencies, standardise protocols, and understand host genetic and environmental influences to optimise their commercial application.

Long-term effects of organic and mineral fertilisation on soil manganese dynamics and agricultural sustainabilityOriginal Paper

Sarfo Kwaku Obeng, Martin Kulhánek, Jiøí Balík, Jindøich Èerný, Ondøej Sedláø

Plant Soil Environ., 2026, 72(3):155-164 | DOI: 10.17221/562/2025-PSE

Manganese (Mn) is often underestimated in plant nutrition. Its availability to plants is influenced by several factors, which can lead to Mn deficiency or toxicity. The objective was to evaluate the transformation of soil Mn over 21 years in a long-term field experiment. Fertilising with (i) sewage sludge 1 (SS1); (ii) sewage sludge 3 (3 times higher nitrogen (N) dose, SS3); (iii) farmyard manure (FYM); (iv) mineral nitrogen, phosphorus and potassium (NPK) and (v) mineral nitrogen in addition to straw (Nst) was studied to evaluate the transformations of Mn in soil using different extraction methods at the 5 locations. There was a general reduction in the pH during the experiment. Soil acidification caused by mineral N fertiliser increased the bioavailable Mn forms under NPK treatment. This Mn was mobilised from soil reserves, leading to depletion of Mn sources. Application of SS and FYM led to an increase in non-bioavailable Mn fractions, while the expected increase in biologically available Mn was not observed. As the high pH of soil limits Mn availability, foliar Mn application can be recommended for agricultural practice in high-pH soils. On the contrary, liming can be recommended for low-pH soil with high bioavailable Mn content to mitigate the risk of Mn toxicity.

Research on the optimal design and process parameters of a castor seed cleaning machineOriginal Paper

Elchyn Aliiev, Valentyn Holovchenko, Olha Aliieva

Res. Agr. Eng., 2026, 72(1):41-58 | DOI: 10.17221/121/2025-RAE


In the context of the modern EU bioeconomy, the use of industrial crops, particularly castor beans (Ricinus communis L.), is relevant for the production of industrial and energy products without competing with food crops. Castor oil is used for the production of biodiesel, lubricants, paints and coatings, cosmetics, and pharmaceuticals. In Ukraine, castor beans can be cultivated on low-yield soils, with seed yields ranging from 1.5 t/ha to 2.1 t/ha depending on the variety. However, the morphological features of the fruits and uneven ripening complicate the mechanisation of harvesting and seed cleaning processes. Existing equipment designed for other crops cannot be applied due to the risk of damaging the castor seeds. A design of a castor seed cleaning machine has been proposed, combining mechanical fruit shelling with the aerodynamic separation of the mixture. The machine is equipped with an eccentric crushing cone, rubber linings, a pneumatic separation channel, a cyclone, and an automated control unit. As a result of numerical modelling and experimental studies of the process of separating and cleaning castor seeds, dependencies were obtained for the productivity of the developed machine Q, power consumption P, specific energy consumption E, fraction of unshelled fruits (segments) ξf, content of the clean seed in the seed collector ψs-s depending on the distance between the reverse and crushing cones δ, rotation frequency of the crushing cone n, diameter of the feed opening Din, inclination angle of the crushing cone axis γ, and airflow velocity V. A multi-criteria optimisation method was applied to find the optimal operating modes: δ = 10.8 mm; n = 282 rpm; V = 3.6 m/s; Din = 98 mm; γ = 3.6°; β = 20.3°. The following results were achieved: E = 0.0394 MJ/kg; Q = 163.4 kg/h; P = 1 861 W; ξf = 0.099; ψs-s = 0.958. The obtained results confirm the efficiency of the proposed design for industrial implementation.

A reproducible rat model for warfarin studies: Prothrombin time assessment with minimal blood using the CoaguChek XS and determination of an effective warfarin dosageOriginal Paper

ET Akyol, MT Hekim, M Erol

Vet Med - Czech, 2026, 71(6):245-253 | DOI: 10.17221/4/2026-VETMED

Warfarin research in rats is often challenged by methodological inconsistencies and large blood volume requirements. This study aimed to validate a refined, reproducible rat model for serial prothrombin time (PT) monitoring using the CoaguChek XS, in alignment with the 3R principles, and to determine an effective warfarin sodium dosage. Male Wistar albino rats received daily oral warfarin (0.125–0.750 mg/kg) for 5 days. PT was measured using the whole-blood CoaguChek XS and two laboratory plasma assays (Innovin, Thromborel S). Agreement was assessed using Bland–Altman and Passing–Bablok regression. The CoaguChek XS consistently yielded higher PT values than laboratory tests but showed strong agreement up to 25 seconds. A daily dose of 0.125 mg/kg resulted in a stable anticoagulant effect without mortality. Doses Doses of 0.250 mg/kg and higher were toxic, causing PT prolongation beyond the device’s range and high mortality within 4–7 days after the initiation of treatment. In conclusion, the CoaguChek XS is a reliable tool for experiments requiring repeated measurements. Values remain reliable up to 25 s, and 0.125 mg/kg is a safe dose for short-term studies, effectively supporting animal welfare and the 3R principles.

An investigation of the presence of Xylella fastidiosa in Cicadomorpha specimens collected in different habitats in PolandOriginal Paper

Monika Ka³u¿na, Gra¿yna Soika, Wojciech Warabieda

Plant Protect. Sci., 2026, 62(2):146-162 | DOI: 10.17221/207/2024-PPS


A quarantine organism, the bacterium Xylella fastidiosa (Xf), is a xylem-inhabiting, vector-transmitted, Gram-negative, and very slow-growing bacterium in the Lysobacteraceae (earlier Xanthomonadaceae) family. The spreading of X. fastidiosa over long distances occurs mainly via import/export human-mediated transportation of mainly latently or symptomatically infected plant material. Short-distance distribution is usually by xylem sap-feeding insects. Until now, the presence of X. fastidiosa has not been reported or studied in Poland. During our study, over 500 individuals from the four families: Cicadellidae, Aphrophoridae, Delphacidae and Membracidae were collected in different geographical regions of Poland. Real-time PCR with primers for rimM gene and nested PCR to detect X. fastidiosa, using DNA extracted directly from selected insects known as potential vectors of X. fastidiosa, did not confirm the bacterium's presence in these insects.

Assessing the links between climate change resiliency and food security in northwestern region of BangladeshOriginal Paper

Abhi Sarker, Salman Ibn Yasin, Bikash Chandra Ghosh, Gershom Endelani Mwalupaso, Asma Akter, Emmanuel Kiprop, Liu Zhenzhen, Farjana Eyasmin, Geng Xianhui

Agric. Econ. - Czech, 2026, 72(6):358-380 | DOI: 10.17221/218/2025-AGRICECON

Climate change is intensifying threats to food security across the developing countries, pushing vulnerable nations like Bangladesh towards a breaking point – where resilience is no longer an option but a survival imperative. Despite growing concerns, however limited empirical research exists on how climate resilience influences food security across rural and urban farming households. This study examines climate resilience in northwestern Bangladesh and its impact on farming households' food security. Using multistage random sampling, 498 households across 16 villages in extreme climate zones were surveyed. A climate resilience index (CRI) was developed to assess resilience, and a binary logistic model analysed its effect on food security; the findings indicate a positive association between them. Other key determinants of food security include household income, non-farm employment, crop diversity, education level of the household head and farm size. Also, urban households exhibit greater resilience than rural ones, with 38.7% and 32.8% classified as food secure, respectively. Enhancing climate resilience through adaptive strategies can improve food security, while off-farm activities provide critical financial support. Policy interventions, such as government or NGO-led agricultural financing, could further strengthen food security.

The effect of cattle breed on the quality of intramuscular fatOriginal Paper

Natalie Klessy, Eva Straková

Czech J. Anim. Sci., 2026, 71(4):170-179 | DOI: 10.17221/29/2026-CJAS


The aim of the study was to verify whether differences in intramuscular fat quality, evaluation based on fatty acid profile and ratios, occur between cattle genotypes – the combined performance cattle breed Czech Fleckvieh and the beef breed Aberdeen Angus – under identical rearing conditions (same pasture location and nutritional management) with extensive grazing. The results of observation show the difference in the quality of intramuscular fat of bulls with combined performance and bulls with meat performance in extensive pastoral farming. Breed differences were reflected by a statistically significantly higher mean intramuscular fat (P ≤ 0.05) in the beef cattle breed (149 ± 60.1 g/kg dry matter) compared to the combined performance breed (120 ± 51.8 g/kg dry matter). A higher saturated fatty acid (SFA) value of 53.4 ± 22.2 g/kg dry matter was observed in the beef cattle breed compared to SFA 40.8 ± 18.9 g/kg dry matter (P ≤ 0.05) in the combined performance cattle breed; a higher monounsaturated fatty acid (MUFA) value of 43.8 ± 26.3 g/kg dry matter compared to a MUFA value of 42.3 ± 20.8 g/kg dry matter (P > 0.05); and a higher value of polyunsaturated fatty acids n-3 (PUFA n-3) 0.76 ± 0.33 g/kg dry matter (P ≤ 0.05) compared to PUFA n-3 0.48 ± 0.21 g/kg dry matter in combined performance cattle breed. A higher value of polyunsaturated fatty acids n-6 (PUFA n-6) 3.66 ± 1.39 g/kg dry matter was found in the combined performance cattle breed compared to the meat cattle breed with PUFA n-6 3.54 ± 0.96 g/kg dry matter (P > 0.05). A more favourable n-6/n-3 PUFA ratio was observed in the Aberdeen Angus (P ≤ 0.05). The results suggest that the different genotypes of the bull breeds studied, reared on extensively used pasture, may influence the amount of intramuscular fat and its quality.

Decision Support Systems in dairy cows farming: A 20-year scoping review of characteristics, applications, and future challengesReview

Jan Saro, Jaromír Ducháèek, Ludìk Stádník, Helena Bro¾ová

Czech J. Anim. Sci., 2026, 71(5):191-207 | DOI: 10.17221/40/2026-CJAS


Decision Support Systems (DSS) streamline dairy farm management by addressing challenges in productivity, animal welfare, sustainability, and economics. Yet, their precise impact on dairy cattle farm operations remains unclear. This scoping review systematically analyses DSS applications in dairy farming using studies from Scopus and Web of Science published between 2005 and June 2025, following PRISMA-ScR guidelines. From 1 112 identified records, 84 studies were included, after deduplication and screening, and classified into four mutually exclusive primary categories, namely data-, model- and knowledge-driven and other specialised DSS. The findings revealed that DSS complexity increased over time, with model-driven systems dominating (40.5%), followed by data- (38.1%) and knowledge-driven (15.5%) DSS, while other specialised systems accounted for the remaining 6.0%. Temporal multi-label analysis also highlighted trends towards integrated methodologies, with 20 DSS combining data- and model-driven approaches. DSS are mainly applied in Animal Health and Welfare (48% model- and 32% data-driven) and in Farm Business and Management (54.5% model- and 22.7% data-driven). Consequently, the top data inputs are Animal Health & Performance (28.0%), Farm & Business (22.4%), and Environmental & Spatial Data (21.3%). The most commonly applied models are Mathematical/Deterministic (22.7%) and Simulation (13.6%) models, increasingly alongside ML techniques. Key challenges include data integration, real-farm validation, model interpretability, bias reduction, and practical usability. Bridging these gaps will enhance DSS effectiveness and strengthen their potential to optimise dairy farming.

Effects of red oak (Quercus rubra L.) and Scots pine (Pinus sylvestris L.) on early soil development during afforestation of nutrient-poor post-mining sandsOriginal Paper

Ondøej ©pulák

J. For. Sci., 2026, 72(6):297-307 | DOI: 10.17221/29/2026-JFS

The article aims to raise the knowledge on how the Northern red oak affects the soil formation and nutrient cycling compared to Scots pine during the early-stage reclamation of nutrient-poor mineral soil that originated in the deep soil layers in a former sand quarry. Furthermore, the aim is to also answer the question if the admixture of red oak helps to regenerate the soil properties more effectively than pure stands. Three treatments were established: pure red oak, pure pine, and a mixed stand, where organic [composite samples of L+F (litter and fermented), F+H (fermented and humic)] and mineral (A, C) soil horizons were analysed. The oak significantly outperformed the pine in the soil amelioration, showing elevated pH, higher base saturation, and enriched concentrations of basic cations in the humus horizons. The lower litter accumulation under the oak reflected the enhanced decomposability of its litter. A pattern of reduced cation exchange capacity, but the elevated base saturation of the oak soils suggested the development of a nutrient-rich microenvironment with a diminished risk of aluminium toxicity. Phosphorus remained critically limiting. The mixed stand exhibited intermediate properties closer to the pine stand, with the synergistic effects currently limited. Our results support red oak as an effective reclamation species for infertile post-mining soils, though sustained amelioration requires a decades-long perspective and integrated forest management strategies.

Empowering farmers through agricultural supply chains: An ESR and IVQR analysis of income effects in ChinaOriginal Paper

Zhao Ding, Qianyu Zhang

Agric. Econ. - Czech, 2026, 72(7):417-434 | DOI: 10.17221/366/2025-AGRICECON

Integrating farmers into the modern agricultural industrial system is key to sustaining rural household income growth. Based on field survey data from rural areas in three western Chinese provinces, this study uses an endogenous switching regression (ESR) model to analyse the impact of farmers' participation in agricultural supply chains on their income, and explores the heterogeneous effects of credit constraints and agricultural loans on the incomes of participating and non-participating farmers. The results show that supply chain participation significantly increases farmers' total and operational income, while driving the modernisation of agricultural production methods. Specifically, supply chain participation enables farmers to use agricultural loans efficiently to drive income growth and alleviate income constraints caused by credit restrictions. Furthermore, the positive effect of supply chain participation on operational income is more prominent, especially for low- and middle-income farmers. In contrast, the income-increasing benefits of agricultural loans are more pronounced among middle- and high-income farmers, reflecting differences in loan utilisation capacity across income groups.

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