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Scattered Greek juniper (Juniperus excelsa M. Bieb.) trees have ecological values, such as soil protection and soil erosion reduction in the Irano-Turanian region; however, intensive exploitation puts their habitats at risk. Therefore, knowing the soil characteristics of these habitats plays an important role in their management. The aim of this study was to investigate the soil quality of semi-arid woodlands dominated by a pure Juniperus excelsa community. At three sites in the Irano-Turanian region, soil samples were randomly taken from a depth of 0–20 cm in the eastern direction under the crowns of Greek juniper trees and from bare soil. The evaluated physical properties included bulk density (Bd), moisture, sand percentage, silt percentage and clay. Chemical properties included pH, electrical conductivity (EC), organic carbon (SOC), total nitrogen (TN), and nutrient concentrations of bare soil compared to the below-crown soil. Additionally, we investigated basal respiration (BR) and microbial biomass carbon (MBC) to evaluate the influence of Greek juniper trees on soil microbial activity. The bulk density value in samples taken under crowns was significantly lower by 42% compared to bare soil; however, significantly higher soil moisture (+39.8%) was obtained under tree crowns. Although clay and sand content under tree crowns was significantly higher than that of bare soil, sand content under tree crowns was significantly lower than in bare soil. According to our findings, soil pH under tree crowns and in bare soil was 7.13, which increased to 7.67 in bare soil. Soil EC (+15%), SOC (+76%), and TN (+29%) were significantly higher under tree crowns. The concentrations of magnesium and sodium were similar between bare and below-crown soils, but significantly higher phosphorus (+46%), potassium (+41%), calcium (+31.1%), iron (43.3), and zinc (+56.6%) were observed under Greek juniper crowns. Soil microbial activity was higher under tree crowns compared to bare soil, as evidenced by significantly increased BR (+49.1%) and MBC (+43.5%). Our findings indicate that scattered Greek juniper trees can improve soil properties, and their destruction leads to a significant decrease in soil quality. Generally, planting Greek juniper seedlings in degraded areas can help restore the soil quality.

The research was conducted at the Department of Agronomy and Agricultural Extension, Rajshahi University, from December 2021 to April 2022. The objective was to develop a fuzzy expert system for site-specific N fertilisation using leaf colour code (RGB) and irrigation frequencies for maize yield. The experiment encompassed two primary factors: nitrogen fertiliser application rates (N₁: 100%, N₂: 75%, N₃: 50% of conventional rates) and irrigation frequencies (I₁: 100%, I₂: 75%, I₃: 50% of pan evaporation). A completely randomised design (CRD) with three replications was used to arrange the experimental pots, each receiving recommended doses of phosphorus, potassium, and sulfur, with urea applied per treatment instructions. Results revealed significant chlorophyll content and grain yield differences among the various nitrogen fertiliser rates. The highest grain yield (219.27 g·pot^–1) was observed with N₁, whereas the lowest (186.6 g·pot^–1) was with N₃. Similarly, irrigation frequencies significantly influenced chlorophyll content and cob characteristics, with I₁ resulting in the highest grain yield (211.27 g·pot^–1) and I₃ the lowest (184.6 g·pot^–1). Furthermore, the interaction between fertiliser application rates and irrigation frequencies had notable effects on various parameters, leading to the highest grain yield of 227.62 g·pot^–1 with the combination of N₁ and I₁ and the lowest (168.00 g·pot^–1) with N₃ I₃. The agricultural experiments were facilitated using the Matlab fuzzy toolbox, employing the Mamdani inference method. Fuzzy rules were delineated for nitrogen application rates and irrigation frequencies, with three fuzzy sets each. Membership functions were developed utilising Matlab's fuzzy interface system (FIS) editor and membership function editor, optimising leaf chlorophyll content, evaporation rate as input tiger N fertilisation, and irrigation frequencies as output for precise maize production in Bangladesh.

Denitrification, anaerobic ammonium oxidation (Anammox), and ferric iron reduction coupled with anaerobic ammonium oxidation (Feammox) are the nitrogen removal pathways in natural ecosystems. In this study, the differences between these three nitrogen removal pathways in a Phragmites australis covered site (LW), artificial grassland covered site (CD), poplar covered site (YD), and topsoil tillage after harvesting reed site (GD) in the Yinchuan Yellow River wetland were investigated using isotope tracing, metagenome, and quantitative polymerase chain reaction (Q-PCR) techniques. No ³⁰N₂ accumulation was detected in ¹⁵NH₄⁺ addition incubations, indicating that Feammox was weak in all sites, which is consistent with a low abundance of the Feammox functional bacteria Acidimiprobiaceae sp. A6. The denitrification rates were 0.36 (LW), 0.5 (CD), 0.76 (YD) and 0.12 (GD) mg N/kg/day. The Anammox rates were 0.18 (LW) and 0.26 (GD) mg N/kg/day; other sites did not detect Anammox rate. Denitrification was the dominant pathway except for the CD site. The YD site had the highest abundance of denitrification genes, which was consistent with the denitrification rate.

The aim of the study was to define and compare the quality properties of dry-cured heat-treated meat products from the meat of free-living semi-aquatic wildlife species. Eurasian beaver (Castor fiber) and nutria (Myocastor coypus) are wild animals whose presence in the countryside is regulated in the Czech Republic. Basic chemical, microbiological, sensory analyses, and instrumental measurements of the colour and texture of dry-cured sausages (pork, as a control group with 51% lean pork and experimental groups with 51% lean beaver and nutria meat in fresh state) were performed. There were no statistically significant differences (P > 0.05) in microbiological counts per gram between the sausage groups according to the meat used after 3 weeks of storage. In the sensory analysis, beaver sausages were rated as the darkest and least attractive (P < 0.05). The data obtained using the Meullenet-Owens Razor Shear (MORS) test did not show a statistically significant difference (P > 0.05). However, a significant difference (P < 0.05) was measured between the beaver product and the pork and nutria variants using the compression method.

Joint field experiments were established in Southwestern Germany to investigate the potential of herbicide savings on-farm sites with high densities of problematic weed species. From 2020 until 2024, 21 field studies were conducted in maize, soybean and winter wheat, all realised as randomised complete block designs with four replications. Mechanical weeding and two combined chemical with mechanical weeding methods were compared to conventional broadcast pre- and post-emergence herbicide spraying and an untreated control. Weed density, herbicide savings, greenhouse gas emissions and crop yield were determined for all treatments. On average, 142 weeds/m² were counted in the untreated plots. The most frequent weed species were Chenopodium album, Echinochloa crus-galli, Solanum nigrum, Stellaria media, and Veronica persica. Combined chemical with mechanical weed control in soybean and winter wheat was more effective than chemical and mechanical weed control alone. In maize, the combination of hoeing and herbicide application achieved equal weed control efficacy (WCE) as chemical weeding alone. Hoeing removed less intra-row weeds than inter-row weeds. Hoeing and harrowing had low WCE against Chenopodium album and perennial weed species. Combined treatments reduced herbicide use by 24–60% in relation to conventional herbicide treatments. Mechanical and combined weed control achieved equal yield as the conventional herbicide treatment. This study underlines the potential for herbicide savings by integrating mechanical weed control methods.

The 2021 Cyclone Seroja was a category 3 storm that made landfall on Lembata Island, causing extensive damage. This study aims to identify key interpretations of sediment transport related to tropical cyclones (TC) Seroja and past floods using a geopedological approach, estimate the return period through frequency analysis, and determine the rainfall threshold for flooding using HEC-RAS software. Extreme rainfall data from global precipitation model (GPM) (2000–2023) in Wei Laing watershed were analysed alongside LiDAR terrain data, physical and chemical properties of soil, and land cover data. Based on geopedological analysis, the result shows that the erosional-transfer zone of Wei Laing Watershed has thin, loamy, and slightly sandy soils due to erosion and limited pedogenesis. The depositional zone contains flood deposits with abrupt vertical texture changes, reflecting transported coarse grains and finer in-situ sediments. The modern flood deposit (TC Seroja flood deposit) was identified by texture, CaCO₃ content, organic matter, and coarse organic material. The fine-grained flood deposits (≤ 4 cm) are classified as slackwater deposits, consist of silty clay loam and silt loam textures, reflecting deposition under slow-flowing conditions. TC Seroja corresponds to a 50-year return period. Hydrological modelling indicates a 60 mm/day rainfall threshold for flooding, with 77 flood events recorded between 2000–2023. The model is confirmed by thick past flood deposits enriched with coarse organic materials. These findings provide insight into flood dynamics and sedimentary responses, supporting future flood risk mitigation efforts.

Two-year experiments were conducted on two different soil types during 2021/22 and 2022/23 to study the impact of microbiological preparations, Mycor-FAZOS and Vitality-FAZOS, on the yield and quality elements of winter wheat cultivar Maja (Agrigenetics). The experiment was set up in a split-block design with four repetitions across two soil types, with 12 different variants on each soil type. The base plot area was 40 m2, and the effective plot area was 27 m2. The research factors included: A (soil type): A1 – gley soil; A2 – eutric cambisol; B (disease control): B1 – control; B2 – chemical pesticides; B3 – biopreparates (seed treatment + foliar treatment) and C (nitrogen fertilisation): C1 – based on soil analysis; C2 – 70% of recommended application. Variables included grain yield (t/ha), grain protein content (%), hectoliter weight (kg), and plant height (cm). The highest average grain yield was achieved with variant B3C2, with a 12.50% increase compared to variant B2C1. Differences between these variants included 6.0% for protein content, 5.97% for hectoliter weight, and 7.93% for plant height. It was observed that Mycor-FAZOS seed treatment promoted root development, resulting in healthier, taller, more robust plants with a more uniform growth and darker colour than untreated seed variants (indicating increased photosynthesis index). The protection achieved with Vitality-FAZOS biopreparations (fungicidal microorganisms synthesising growth hormones) ensured healthier wheat plants, leading to higher seed yield and better quality parameters.

The paper deals with the assessment of the influence of the length of cold stratification period on the germination parameters of seeds of wild service tree [Sorbus torminalis (L.) Crantz]. The germination tests (4 × 100 seeds) were conducted in two runs in 2020 and 2021; they were performed for three seed lots and three cold stratification periods (two, three and four months) before the testing of germination in each run. The values of germination energy and germination capacity were assessed, and the germination rate was computed. The results showed that the two-month cold stratification period has not been sufficient to overcome the dormancy of tested seeds. Regardless of the year in which the tests were conducted, seeds stratified for two months showed almost zero germination. For seeds treated with three- and four-month cold stratification, the germination percentage varied from ca. 5% to 32% and from ca. 13% to 46%, respectively. Our results basically correspond with findings reported in the reviewed literature. In 2020, the seeds subjected to four-month stratification performed significantly better than the seeds subjected to three-month stratification. However, in 2021, the seeds stratified for three months reached slightly better results. Four-month stratification also led to an increased percentage of prematurely germinated seeds during stratification. To conclude, the two-month period of cold stratification proved to be insufficient and at least three months of cold stratification are needed to obtain the adequate germination of seeds in subsequent laboratory tests.

A 12-year-old castrated male domestic shorthair cat was referred for respiratory distress. Physical examination revealed a systolic heart murmur at the left apex and crackles in all lung fields. Thoracic radiography showed Valentine-shaped cardiomegaly, pulmonary oedema, and pleural effusion. Echocardiography revealed focal thickening of the interventricular septum [11.01 mm; reference interval (RI) = 3.00–5.20 mm] and left ventricular posterior wall (7.41 mm; RI = 3.00–5.10 mm) during diastole. In the apex region, the free wall was focally thinned to approximately 1.6 mm with hypokinetic myocardial movement, indicating thin and hypokinetic myocardial segments. Additionally, decreased left atrial fractional shortening (12.5%; RI = 23.9–34.9%) and an increased left atrial-to-aortic ratio (2.87; RI = 0.88–1.43) were observed, along with spontaneous echocardiographic contrast in the left atrium, indicating increased thrombotic risk. The electrocardiogram showed a left axis deviation with small R waves and deep S waves in lead II, which is consistent with a left anterior fascicular block caused by delayed conduction in the left anterior fascicle. This case report describes the coexistence of a left anterior fascicular block and thin, hypokinetic myocardial segments in feline hypertrophic cardiomyopathy, suggesting a possible pathophysiological link.

As essential natural carbon sinks, woody plants play a key role in urban ecological restoration. The lignite-derived organic fertiliser (LOF) may promote plant growth and carbon sequestration by improving soil properties. This study investigated LOF effects on three typical woody plants – Styphnolobium japonicum (L.) Schott. with taproots, Malus × micromalus Makino with fibrous roots, and Malus domestica Borkh. with both taproots and fibrous roots – focused on soil properties improvement during a three-year planting experiment (2021–2023). The results indicated that LOF application significantly increased soil organic matter (SOM) content, with and without woody plants, by 82.3% and 54.9%, respectively. Concurrently, LOF influenced soil microbial characteristics, especially enhancing the 16S rRNA gene copy number by 0.99 times. For plant growth, LOF application increased root length, volume, and tip number in Malus domestica Borkh. by 37.4, 27.4, and 26.0%, respectively, and in Styphnolobium japonicum (L.) Schott by 43.8, 76.7, and 26.6%, respectively. However, in Malus × micromalus Makino, while root volume increased by 3.8%, root length and tip number decreased by 10.0% and 26.9%, respectively. Additionally, the LOF application increased the soil plant analysis development (SPAD) values of woody plant leaves by 5.3%, indicating improved chlorophyll content and plant health. These findings demonstrate that LOF applications may significantly enhance soil quality and promote plant growth, contributing to improved terrestrial carbon sequestration.

The purpose of this study was to assess the amino acid and fatty acid profiles of swamp buffalo meat (Bubalus bubalis) obtained from a local market. The extractable free amino acids and free fatty acids of raw and cooked loin (Longissimus lumborum: LL) and round (Semimembranosus: SM) buffalo meat were analysed. These experiments were performed in a 2 × 2 factorial in randomised complete block design (RCBD) and there were four treatments combinations, including raw-loin, cooked-loin, raw-round and cooked-round with five replications. The results revealed the amino acid composition for the raw-loin included leucine [4.51 mg·(100 g)^–1 sample) and isoleucine [4.56 mg·(100 g)^–1 sample], whereas methionine, aspartic acid and asparagine were the least common amino acids found in both raw and cooked swamp buffalo meat [0.002, 0.020 and 0.034 mg·(100 g)^–1] of the sample. The amino acid composition of the cooked meat decreased by 50% as compared to raw meat. Swamp buffalo meat contains 10 fatty acids, including saturated fatty acids (SFAs) such as myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0) and docosanoic acid (C22:0); monounsaturated fatty acids (MUFAs) such as palmitoleic acid (C16:1, n-7) and oleic acid (C18:1); and polyunsaturated fatty acids (PUFAs) such as linoleic acid (C18:2, n-6, ω6), gamma-linolenic acid (C18:3, n-6, ω6), eicosatetraenoic acid (C20:5, n-3, ω3) and docosahexaenoic acid (C22:4, n-6, ω6). Stearic acid (C18:0) was the most abundant saturated fatty acids found in the lipid component of buffalo meat. Other medium- and long-chain saturated fatty acids (C14:0, C16:0, C22:0) contributed to around 3–4% of the total fatty acid composition. The most abundant MUFAs and PUFAs were oleic acid (C18:1) and eicosapentaenoic acid (C20:5). The PUFA/SFA ratio, total polyunsaturated fatty acids n-3, total polyunsaturated fatty 64 acids n-6, and n-6/n-3 ratios were not significantly different between raw and cooked buffalo meat.

Assessing the agricultural sustainability of farms is challenging, since it involves various aspects that can change over time and differ by location. This paper develops a composite index to evaluate the sustainability of cereal farming in Catalonia, Spain. Using factor analysis, we integrate 21 indicators across economic, environmental, and social dimensions based on the Farm Accountancy Data Network (2016–2021). The results show sustainability scores ranging from 2 to 5, with larger economic s farms outperforming smaller ones by 0.4 points. Five key factors explain the variance in sustainability across farms, with profitability, benefit-cost ratio, and agri-footprint carrying the highest weights. In addition, our empirical findings indicate that subsidy dependence negatively affects the sustainability of farms, while modernisation and environmental management improvements enhance farm performance. This suggests a need for size-specific policy interventions focusing on smallholder management capacity and broader climate adaptation strategies. The methodology could offer a practical tool for monitoring sustainability progress in Mediterranean cereal production systems, and for identifying possible sources of improvements with regard to more sustainable agricultural practices.

In most developing countries, the agricultural industry has the potential to alleviate poverty at a faster rate compared to other sectors. As such, many governments have initiated policies and programme to improve agricultural performance. However, some of these projects have not achieved success because the local agricultural markets are often controlled by a small group of stakeholders who tend to hide information in the market despite having huge market power. This paper aimed to determine the issues and impacts of the lack of market information in developing countries and to provide strategies for solving such problems. Results show that the presence of an oligopsonic market system, current information and communication technology (ICTs), the lack of market infrastructure and gender limitations are the main issues related to a lack of information. In turn, lack of information leads to an inefficient agricultural market, causes negative impacts on market participants, and ultimately leads to harmful socio-economic effects. Thus, to solve these problems, it is necessary to improve capacity building, increase access to ICTs and improve market infrastructure.

One hundred per cent apple, orange and grapefruit juices were analysed for ascorbic acid (AA) content, total polyphenolic content (TPC), and other parameters: titratable acids (TA), malic acid (MA), citric acid (CA), volatile acid (VA), soluble solids (SS), density, and the content of free amino acid as formol number (FN). Some apple juices on the Czech market contain added vitamin C, and the AA content of such juices (268-632 mg L^-1) is several times higher than that of apple juices without added vitamin C (around 50 mg L^-1). Therefore, the enriched apple juices had a vitamin C content comparable to citrus fruit juices (orange and grapefruit). All contents were compared separately for juices made from concentrate and for directly pressed juices. The effect of the production method was statistically significant (P < 0.05) only for TPC in apple juices, where it was major [473 ± 136 mg gallic acid equivalents (GAE) L^-1 from concentrate vs. about twice the value of 798 ± 193 mg GAE L^-1 in directly pressed juice], and only to a smaller extent for TA in grapefruit juices (12.5 ± 0.8 g L^-1 from concentrate, which was about 30% more than in directly pressed juice).

The paper presents the maps of ecosystems and 11 dominant woody species of Ambrolauri municipality, Georgia (Europe). Forest cover comprises approximately 68% of the study area, most of which are natural forests. Almost all formations of natural forests, depicted on the Georgian section of the Map of the Natural Vegetation of Europe, are still preserved in the research area. Nearly 40% of the forested land is broadleaf forest. Woody species are better preserved in the upper reaches of rivers, while an anthropogenic transformation is most visible in the Lower Racha Floodplain. A majority of the species discussed in the paper are included in the IUCN Red List of Threatened Species. The paper also deals with the issues of forest land degradation and threats imposed by invasive species to biodiversity.

Air quality in Nur-Sultan, the fast-growing new capital city of Kazakhstan, has been poorly investigated. This research was carried in Kerey and Janibek Khans Park in Nur-Sultan, the capital city of Kazakhstan, which is affected by "different traffic density" on the roads. Three different categories of contamination (i) high pollution (distance from the main road 10-15 m), (ii) moderate pollution (distance from the side road 15-30 m), and (iii) low pollution (distance from the source of contamination 30-80 m) at different levels resulting from urban transportation were examined. The aim of the study is to determine the pollution accumulation amounts of Ni, Cd, Pb, Cr, Li, Co, Fe, and Zn in five different tree species: Norway spruce (Picea abies /L./ H. Karst.), blue spruce (Picea pungens Engelm.), Scots pine (Pinus sylvestris L.,), Siberian larch (Larix sibirica Ledeb.), and silver birch (Betula pendula Roth). Significant differences in Ni, Cd, Pb, Cr, Li, Co, Fe, and Zn accumulation amounts were determined between three different parts of the park exposed to different levels of contamination. Approximately twice higher Ni, 2.3 times Cd, 3.3 times Pb, 2.7 times Co, 1.6 times Zn accumulation were determined in the area exposed to high pollution than in the parts of the park exposed to low pollution. Cd deposition in the area exposed to high contamination was higher in silver birch than in Scots pine and Norway spruce, while Cr and Co deposition values were higher in Norway spruce than in birch and Scots pine.

The objective of this research is the identification of burned forest areas that occurred in Syria from September 2^nd to October 15^th, 2020. Forest fire risk classes were determined using Sentinel-2 images. Normalized Burn Ratio (NBR), Differenced Normalized Burn Ratio (dNBR), and Burned Area Index for Sentinel-2 (BAIS2), and Normalized Difference Vegetation Index (NDVI) were used for the identification how much the forests have been destroyed and to establish fire risk classes. According to the study results, the size of the vegetation area that was destroyed due to fire was determined, and the probability of the forest fire exposure of these areas was established. The fires also altered some chemical properties in the soil during the combustion process. Thus, this study was focused on the impact of fire on the availability of soil nutrients. Soil samples were collected from three depths (0-10 cm, 10-20 cm and 20-30 cm) under the forest land a month after the fire in three locations: Al-Fazeen, Sawda and Gard Al-rihan. Pine (Pinus brutia) trees cover these areas. The results of this study indicated that the fire increased pH, EC and sand, the fire also led to an increase in the solubility of the available major soil elements N, P and K. There was an increase in the solubility of the soil microelements Zn, Cu, Mn and Fe while the content of organic material and silt and clay ratio decreased at the three sites in comparison with unburned soil.

The double-spined bark beetle Ips duplicatus (Coleoptera: Curculionidae, Scolytinae) is an invasive forest pest having a broad range of coniferous hosts within Europe. We found this species to be also developing in the Serbian spruce Picea omorika. Ips duplicatus infested 14 (52%) of 27 P. omorika mature trees in an arboretum garden in northern Slovakia in Central Europe during the summer of 2019. Logs from the upper part of stems of P. omorika trees placed in eclectors in the laboratory, yielded a total of 179 individuals of three scolytine species, with prevalence of I. duplicatus. Our results show that I. duplicatus also colonized less likely host such as the Serbian spruce, causing threat to this ornamental tree and contributing to its mortality in urban habitats during hot and dry summer weather.

The population structure of Phytophthora capsici among asexual and sexual progenies was analyzed using ISSR. Thirty asexual progenies of one parent and three sexual populations were assayed for genetic diversity using 5 ISSR primers and DNA from 120 offspring of P. capsici was amplified. In total, 71 reproducible ISSR fragments were obtained, of which 100% were polymorphic, revealing high polymorphism among the isolates. Among them, the percentages of polymorphism of sexual and asexual progeny isolates were 100.00 and 77.46%, respectively. Genetic similarity coefficients among all the isolates ranged from 0.54 to 0.73. The sexual offspring population showed much more variability than the asexual offspring population with 76.26% variability attributed to diversity within populations as compared with 23.74% among populations. This research reveals that the sexual progeny population of P. capsici contributes more genetic diversity than that of asexual progeny population.

New techniques for Ceratitis capitata (Wiedemann, 1824) control are being studied and developed to replace traditional organophosphate pesticide applications. A mass trapping strategy offers promising medfly control within integrated pest management (IPM) programmes. Field assays were performed to study the efficacy of two mass trapping techniques based on PheroNorm^® and Ceratrap^® lures that were compared to a conventional approach to control the medfly in Tunisian apricot orchards. The results showed that both mass trapping techniques had a similar efficiency in reducing the C. capitata population. The degrees Brix in the fruits was a determinant issue since the initial apricot fruit damage was detected at 6.4%. At harvesting, the lowest fruit damage rate was recorded in the PheroNorm^® (4.25%) and Ceratrap^® (6.50%) treated orchards, compared with the conventional approach (10.75%). Therefore, the use of 50 PheroNorm^® and Ceratrap^® traps per ha density within an IPM approach may be very useful to control the C. capitata populations in apricot orchards.

The soybean (Glycine max) is an important crop. The pod borer (Etiella zinckenella) is one of the most serious insects that attacks various Leguminosae. Common insecticidal controls are ineffective because of the insect's growth properties. Use of resistant crop varieties offers stabilisation of the yield and has benefits over the use of insecticides. Bacillus thuringiensis is widely used as a bioinsecticide for pest control and a genetic material for pest-resistant transgenic plants. However, the resistance evolution of target insects is emerging as a major threat to the long-term efficacy of these applications. Studies on the detection of novel highly host-specific pesticidal proteins have been in urgent demand. A search for the source of Bt Cry toxins against E. zinckenella in the Vietnamese B. thuringiensis strain collection has been performed. The B. thuringiensis serovar canadensis SP142 is one of strains that resulted in more than 80% mortality to this pod borer. Its genome was estimated about 7.1 Mb and revealed a putative novel cry2Ab gene. The sequence analysis of cry2Ab gene revealed an open reading frame of 1 899 bp encoding a 633-amino acid protein with a calculated molecular mass of 70 kDa and 99.05% to 99.21% homology to known cry2Ab genes in the GenBank. There are eighteen different nucleotide sites which lead to five amino acid changes in Domain I and II. This gene was expressed in Escherichia coli BL21(DE3) and the purified Cry2Ab was toxic to E. zinckenella larvae with an LC₅₀ value of 1.74 µg/g diet. The novel Cry2Ab was designated as Cry2Ab39 by the Bacterial Pesticidal Protein Resource Center and its sequence was deposited in the GenBank (MN319700.1). This is a type of novel Cry2 toxin from B. thuringiensis against E. zinckenella, and it is important for breeding E. zinckenella-resistant soybeans.

In the present work, the phenology of the most important peach pests was studied to estimate the risk thresholds to be used in applying an updated automatic pest management decision support system. Particularly, the seasonal occurrence of Anarsia lineatella, Grapholita molesta and Adoxophyes orana was determined during 2018 and 2020 using pheromone traps from April till October in eight peach orchards in the prefecture of Pella in northern Greece. Additionally, the accumulated degree-days (DDs) were calculated for each moth and further related to the seasonal flight patterns to determine the period of which the activity of each species starts as well as the period of the moth population peak. Moth capture data of one more year, 2021, were used to validate the risk threshold predictions. In most cases, the risk threshold predictions were at acceptable levels and especially in forecasting the start and the peak of the first and second flight period of the above three species. The first captures of A. lineatella, G. molesta and A. orana, early in the season were observed at 70, 33 and 362 DDs, respectively (lower temperature thresholds: 11.4 °C, 9.5 °C and 7.2 °C, for A. lineatella, G. molesta and A. orana, respectively, and Biofix: 1^st of January in all the cases). The highest number of moth captures of A. lineatella, G. molesta and A. orana were observed at 150.6, 77.9 and 428.7 DDs, while the start of the subsequent second flight was observed at 365, 133 and 362 DDs, respectively. Moreover, the peak of the second moth flight was observed at 511.5, 204.8 and 1 239.5 DDs, for A. lineatella, G. molesta and A. orana, respectively. The current degree-day risk thresholds can be used for the precise timing of pesticides and are a prerequisite to implement automated real time decision support systems at a farm level.

Sustainable crop production requires an efficient usage of fossil energy. This six-year study on a silt loam soil (chernozem) analysed the energy efficiency of four tillage systems (mouldboard plough 25-30 cm, deep conservation tillage 35 cm, shallow conservation tillage 8-10 cm, no-tillage). Fuel consumption, total energy input (made up of both direct and indirect input), grain of maize yield, energy output, net-energy output, energy intensity and energy use efficiency were considered. The input rates of fertiliser, herbicides and seeds were set constant; measured values of fuel consumption were used for all tillage operations. Total fuel consumption for maize (Zea mays L.) production was 81.6, 81.5, 69.5 and 53.2 L/ha for the four tillage systems. Between 60% and 64% of the total energy input (17.0-17.4 GJ/ha) was indirect energy (seeds, fertiliser, herbicides, machinery). The share of fertiliser energy of the total energy input was 36% on average across all tillage treatments. Grain drying was the second highest energy consumer with about 22%. Grain yield and energy output were mainly determined by the year. The tillage effect on yield and energy efficiency was smaller than the growing year effect. Over all six years, maize produced in the no-tillage system reached the highest energy efficiency.

To provide reference for scientific management of nitrogen (N) fertiliser on tartary buckwheat, the effects of the mixed application of controlled-release N fertiliser (a kind of thermoplastic polymer-coated urea types that are characterised by a semi-permeable membrane) and common urea was studied in the main tartary buckwheat production area in China. In 2018 and 2019, a two-year field experiment was conducted a randomised block design with five treatments: (1) no nitrogen fertilisation (CK); (2) 100% N from common urea (T1); (3) 15% N from controlled-released urea fertiliser (plastic coated) + 85% N from common urea (T2); (4) 30% N from controlled-released fertiliser + 70% N from urea (T3); (5) 45% N from controlled-released fertiliser + 55% N of urea (T4). The N fertilisation rate was 90 kg N/ha in all fertilisation treatments. The results showed: (1) the mixed application of controlled-release N fertiliser and common urea was conductive to enhance the yield, dry mass, N uptake and apparent N fertiliser efficiency (NFE), compared with a single application of common urea. In two seasons, NFE was 38.6% (T1), 48.6% (T2), 53.6% (T3) and 53% (T4), separately; (2) the mixed application of controlled-release N fertiliser and common urea could significantly increase the soil inorganic N content in the soil surface layer and decreased the leaching loss of N; (3) with the increasing ration of controlled-release N fertiliser, the tendency of increasing N content of crop uptake and soil residual and decreasing rate of N loss and N surplus was visible. Overall, considered the indicators of grain yield, input cost, N utilisation and N balance, the suitable N fertilisation mode for tartary buckwheat production is the mixed application of 30% controlled-release N fertiliser and 70% common urea when 90 kg N/ha is applied.

Among the various disposal strategies for sewage sludge (SS), soil application is the most suitable. This study was conducted to evaluate soil amendment with SS (0, 10, 20, 30 and 40 g/kg) and its impact on soil fertility and tomato (Solanum lycopersicum L.) growth. The SS significantly improved the agromorphological attributes, the number of produced fruits, and the fruit biomass of tomato plants. The 30 g/kg application of SS led to the highest growth rate and fruit yield. Considering the fruits, the best safe enrichment of metal nutrients was recorded at 30 g/kg, with a significant increase in the micronutrient metals Mn, Zn, Ni, Cu, and Fe with 624, 193, 125, 70, and 32%, respectively, compared to the control. The SS amendment enhanced soil fertility, and heavy metals were within the permissible ranges for agricultural soils. Bioaccumulation factors (BFs) indicated that SS application induced the accumulation of most of the studied metals in the roots, and the BF values of Zn, Cu, Ni, and Pb were > 1. The current study concluded that recirculating SS nutrient components to agricultural soils could offer a valid solution for the sustainable management of this organic waste and enhance plant-crop productivity.

Recently, plant-available sulphur (S) in the soil is decreasing due to the limited use of S containing fertilisers and the reduction of atmospheric S deposition. The aim of this work was to evaluate the S status in a long-term fertilisation experiment on a Chernozem in Hungary, with control and 2 NPK rate treatments, considering that after 27 years of superphosphate (SP) use, SP was replaced by monoammonium phosphate in 2010. Plant and soil sampling were performed in 2017 at three different development stages of winter wheat. To assess the S status, the S balance was estimated (for 34 years), KCl soluble soil sulphate, S as well as nitrogen (N) concentration and some amino acids in wheat grain were measured. N/S ratios, S and N uptake of wheat were calculated. The residual effect of SP could be measured only in terms of KCl soluble SO₄^2--S in soil. According to the wheat grain S concentration (0.08-0.10%) and N/S ratio (14.9-22.0), wheat was S deficient, despite the positive S balance in the fertilised plots. In this experiment, where S fertiliser was applied with 84 kg S/ha dose in 1983-2010, followed by a 7-year period without S fertilisation, S supply is necessary for achieving adequate wheat quality.

Microplastics (MPs) are plastic particles smaller than 5 mm in size, which are widely present and have become one of the major pollutants in the natural environment, and are increasingly recognised as emerging pollutants in agricultural ecosystems. Due to their small size and high mobility, MPs can easily migrate into farmland soils and attach to plant surfaces, thereby altering the physical, chemical and microbial properties of the soil. These changes may affect seed germination, plant growth, and physiological and biochemical functions. This review systematically synthesises current research on the impact of MPs on agricultural soil, focusing on their effects on soil structure, chemical properties and microbial diversity. The positive and negative effects of MPs on plant seed germination, growth, and physiological and biochemical processes are critically analysed. Furthermore, the potential ecological risks of MPs to soil and plant health are discussed. Mitigation strategies and future research priorities are proposed to address MPs contamination in agricultural systems. This study aims to provide both theoretical insights and practical references to support the prevention and control of MPs pollution in farmland soils, thereby contributing to sustainable agricultural development and soil ecosystem resilience.

The study aimed to evaluate the concentration of potentially toxic elements in the soil samples of landfill sites in An Giang province. Eighty-eight soil samples were collected from five landfills. The potentially toxic elements including Cu, Zn, Pb, Cd, and As were analysed and compared with the National technical regulations on the allowable limits of heavy metals in the soils. A cluster analysis was applied to identify the sampling sites with similar soil toxic elements properties. The ecological potential risk index was used to determine the risk of the landfills to the ecosystem. The results showed that four out of five potentially toxic elements were detected in the soil, and their concentration decreased in the order of Zn > Cu > Pb > As. Most of the potentially toxic element concentrations were within the allowable limits, except for Cu and As in some positions. The ecological potential risk index in unsanitary landfills was higher than that in a sanitary landfill; however, the level of risk was low. The occurrence of toxic elements in the soil around the landfills affects the ecosystems as well as human health. Therefore, it is necessary to prevent the effect of heavy metals in the surrounding environments.

Nitrogen (N) is essential for crop productivity, yet excessive fertilisation decreases the nitrogen use efficiency and increases the environmental risks. Despite the economic and medicinal importance of the shallot, its molecular response to N deficiency remains unclear. We performed transcriptome profiling of two cultivars (Bima-0 and Bima 1) under N deficient and N-sufficient conditions. The RNA sequencing identified 438 differentially expressed genes (DEGs). The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed significant modulation of the nitrogen metabolism, amino acid biosynthesis, glutathione metabolism, and stress responses pathways, indicating coordinated metabolic reprogramming under N limitation. The cultivar-specific expression patterns suggested differential adaptive strategies. The quantitative real-time polymerase chain reaction (qRT-PCR) validation of fifteen DEGs confirmed the reliability of the RNA-Seq results. The nitrogen deficiency predominantly repressed the photosynthetic and growth-linked genes while activating the stress-responsive pathways. These findings provide the first transcriptome-level insights into the N deficiency responses in shallots and identify candidate genes and pathways for improving the nitrogen use efficiency (NUE) and developing the sustainable fertiliser management of Allium crops.