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Results 331 to 360 of 4875:

Silver fir restitution: The role of seedling stock type in adapting to various environmental conditionsOriginal Paper

Wojciech Kowalkowski, Rados³aw Jagie³³o, Marlena Baranowska, Wladys³aw Barzdajn

J. For. Sci., 2025, 71(7):358-371 | DOI: 10.17221/28/2025-JFS

Due to past human activities, Abies alba Mill. (European silver fir) now covers only 0.7% of the forest area in the Sudety Mountains. A large-scale restitution program has been launched to produce, plant, and effectively protect over 200 million seedlings. This study aims to validate commonly used seedling stock types and provide critical insights into identifying the most effective one. Three-year-old bareroot seedlings were produced in the open-canopy nursery (3/0) or under-canopy nurseries in Scots pine (Pinus sylvestris L.) (3/0 Sp) or Norway spruce [Picea abies (L.) H. Karst] (3/0 Ns) stands. Two stocks were produced in a greenhouse and transplanted to an open-canopy nursery (2/1) after the second year or into containers designed by Kosterkiewicz (2/1 K). Seedlings have been planted in four regions in the Sudety Mountains. Two performance characteristics have been measured: height and survival rate. Our findings demonstrate a clear gradient in sapling performance among seedling stock types (survival rate): 2/1 K (81%), 3/0 (73%), 3/0 Sp (73%), 2/1 (70%), 3/0 Ns (62%). This paper has shown that under favourable environmental conditions, bareroot seedlings can sufficiently achieve stable regeneration. Containerised seedlings perform better in harsher and more challenging microclimatic or trophic conditions. Kosterkiewicz's method presents a relatively low-cost and environmentally friendly alternative for silver fir restitution.

Assessing stem rust tolerance in commercial wheat varieties: Insights from field trials in KazakhstanOriginal Paper

Akerke Maulenbay, Aralbek Rsaliyev

Plant Protect. Sci., 2025, 61(4):333-349 | DOI: 10.17221/219/2024-PPS


This study provides the first comprehensive assessment of stem rust tolerance in commercial wheat varieties from Kazakhstan and Russia, including spring and winter varieties. Field trials were conducted to compare yield and agronomic traits between stem rust-inoculated and fungicide-treated plots, providing a practical framework for assessing tolerance. Key indicators such as disease severity, area under the disease progress curve, thousand kernel weight, and the stress tolerance index were evaluated to gauge variety resilience under stress. Significant variations in tolerance were observed, with varieties such as 'Pamyat' 47', 'Nadezhda', 'Lyubava 5', 'Tselinnaya 3s', 'Severyanka', 'Egemen-20', 'Zhemchuzhina Povolzh'ya', 'Dimash', 'Serke' and 'Korona' maintaining yield potential despite high disease pressure. Correlations revealed that traits such as flag leaf area, vegetative period, and plant height were associated with greater tolerance, highlighting their potential in breeding. With the expected increase in stem rust outbreaks due to climate change and the evolving virulence of stem rust pathogens, these findings emphasise the need for breeding programs incorporating resistance and tolerance, offering a sustainable alternative to fungicide use. This study provides critical insights for breeders and plant pathologists seeking to enhance wheat resilience in regions prone to rust epidemics.

Physiological and biochemical bases of AMF-mediated antimony stress tolerance in Linum usitatissimum: enhancing growth, phytochemical production, and oxidative damage resilienceOriginal Paper

Ahlem Zrig, Shereen M. Korany, Hana Sonbol, Emad A. Alsherif, Foued Hammouda, Danyah A. Aldailami, Marwa Yousry A. Mohamed, Mohamed S. Sheteiwy, Maria Gabriela Maridueña-Zavala, Salma Yousif Sidahmed Elsheikh

Plant Soil Environ., 2025, 71(9):650-665 | DOI: 10.17221/246/2025-PSE

Antimony (Sb) pollution from industrial activities poses a severe global threat, particularly impacting valuable medicinal crops like linseed, which are highly sensitive to heavy metals. This study reveals the remarkable potential of arbuscular mycorrhizal fungi (AMF) as a sustainable solution to this challenge. Our research demonstrates that while Sb stress significantly impairs linseed growth and photosynthesis, it also triggers oxidative damage. AMF improved photosynthetic performance and water status, and notably enhanced the biosynthesis of crucial phytochemicals like phenolics, flavonoids, and citric acid. These compounds are vital for both plant defence and human health. Furthermore, AMF promoted the accumulation of essential detoxifying agents, leading to a better redox balance and significantly reducing Sb uptake and translocation by 47%. This dual action not only bolsters the plant’s tolerance to Sb but also enhances its medicinal value by boosting health-promoting bioactive metabolites. These promising findings underscore AMF’s dual role: a powerful tool for phytoremediation and a natural enhancer of phytochemical quality. Arbuscular mycorrhizal fungi provide a sustainable, nature-inspired approach to safely cultivate medicinal plants in environments contaminated with heavy metals, underscoring the vital role of plant-microbe interactions in alleviating environmental stresses.

Unearthing the hidden domain of epicormic shoots: Insights into forest management impacts on Quercus petraea (Matt.) Liebl.Original Paper

Igor ©tefanèík, Stanislav Vacek, Zdenìk Vacek, Michal Kubìnka, Václav ©imùnek, Josef Gallo

J. For. Sci., 2026, 72(1):28-41 | DOI: 10.17221/2/2026-JFS

Forest management of durmast oak stands [Quercus petraea (Matt.) Liebl.] is focused on the production of high-quality assortments for the furniture industry. Due to various forest management factors and impacts of climate change, their vitality and quality are often reduced. Sudden illumination of the oak trunk caused by management cuts can lead to epicormic shoot formation. This study compared two localities and nine long-term research plots at the stand age of 62–68 years in Slovakia. While one locality was affected by the massive dieback of oak in the past, the other was not. The epicormic shoot occurrence was monitored separately in the lower and upper parts of the trunk, depending on selected quantitative tree parameters and on the various management methods. The formation of epicormic shoot was influenced especially by DBH, crown width and crown volume. The occurrence of shoots was always markedly higher in the upper part of the trunk compared to the lower one. During a 35-year period, the development of the proportion of epicormic shoots in the lower part of the trunk in the locality with massive dieback of oak confirmed a higher occurrence on control plots compared to the managed plots with thinning interventions. It showed that the occurrence of epicormic shoots in durmast oak stands was influenced not only by different methods of management but also by the dieback effect in the past. Promoting more intensive thinning in early-stage oak stands is advised, as it prompts trees to develop larger crowns with limiting formation of epicormic shoots.

Endogenous inhibitory compounds in bovine milk, their significance and methods of detection

Marcela Klimesova, Ludmila Krizova, Hana Nejeschlebova, Oto Hanus, Lenka Vorlova

Czech J. Anim. Sci., 2025, 70(12):499-517 | DOI: 10.17221/146/2025-CJAS


Raw cow’s milk is one of the most strictly controlled commodities in the food industry. Besides basic hygienic and nutritional parameters, the presence of inhibitory and contaminant substances plays a critical role in technological quality and safety. While the vast majority of inhibitory residues are represented by antibiotics originating from mastitis prevention and therapy, milk also contains a broad spectrum of naturally occurring native antimicrobial compounds. These include immunoglobulins, lactoferrin, lysozyme, lactoperoxidase, phosphatases, lipases, and proteases acting as integral elements of the innate immune system within the mammary gland. Although these substances are not legislatively regulated, they significantly influence milk processing, starter culture growth, cheese yield, and shelf-life of final dairy products. Their concentration is strongly associated with animal health status, especially mastitis, and may thus also serve as an indirect quality indicator. This review provides a comprehensive overview of native inhibitory compounds in bovine milk, their concentration ranges, biological significance, impact on dairy technology, and the analytical methods currently used for their detection.

Sustainable agricultural performance in the European Union in the context of ecological transition, economic fairness, and support policiesOriginal Paper

Valentin Marian Antohi, Jean Vasile Andrei, Costinela Fortea, Dumitru Nancu

Agric. Econ. - Czech, 2026, 72(7):435-460 | DOI: 10.17221/329/2025-AGRICECON

This paper investigates the structural determinants of sustainable agricultural performance across the 27 European Union member states over the period 2012–2023, focusing on the interplay between economic efficiency, environmental sustainability, and social equity. Employing a panel quantile regression approach, the study captures the heterogeneous effects of organic farming practices, public support for agricultural research and development, income inequality, and environmental emissions on multiple dimensions of agricultural performance.  The analysis underscores the importance of empirical evidence in designing integrated agricultural policies that enhance sustainability, competitiveness, and social cohesion, in alignment with key European strategies, including the Common Agricultural Policy 2023–2027, the European Green Deal, and the Farm to Fork strategy. The findings reveal that the influence of structural determinants varies significantly across the performance distribution, with stronger effects observed in lower-performing contexts. These results support increased public investment in agricultural research and innovation, the development of customised incentives for organic farming tailored to performance levels and farm structures, and the implementation of redistributive mechanisms to mitigate regional disparities in agricultural income. Higher agricultural income is consistently associated with greater value-added efficiency and lower ecological intensity, suggesting structural decoupling between economic growth and environmental pressure. Overall, the study highlights the need for targeted, data-driven policy interventions to foster innovation, resilience, and inclusive rural development across the European Union.

Insights into panicle trait variation and DUF-640 gene conservation in Indonesian foxtail millets (Setaria italica)Original Paper

Marisa Vidya Luthfiani, M. Reza Pahlevi, Bambang Sapta Purwoko, Sintho Wahyuning Ardie

Czech J. Genet. Plant Breed., 2025, 61(4):235-246 | DOI: 10.17221/86/2025-CJGPB

Foxtail millet (Setaria italica) is a resilient yet underutilised C4 cereal valued for its adaptability to abiotic stress and high nutritional content. While panicle traits have been linked to yield in many cereals, the spatial arrangement of foxtail millet panicles remained unexplored, especially among locally adapted genotypes. This study aimed to characterise spatial panicle architecture traits and to analyse the DUF-640, a gene controlling primary branch number, among Indonesian foxtail millet genotypes. Results revealed substantial variation in panicle architecture, including primary branch number, grain number, and grain density in eight Indonesian foxtail millet genotypes, suggesting potentially greater diversity across broader germplasm. In contrast to the substantial panicle trait variations, phylogenetic and structural analyses showed that DUF-640 genes were highly conserved across Setaria species. Although previously associated with primary branch development, the coding sequence of DUF-640 was not associated with branching variation in this study. However, its high sequence conservation across Setaria species suggests a vital and possibly conserved regulatory function. This study enhances the understanding of the morphological and genetic diversity of foxtail millet, particularly among Indonesian foxtail millet genotypes. Future research should focus on the functional characterisation of DUF-640 and the identification of regulatory sequences governing its gene expression.

Remediation of cadmium-polluted acidic soil with dolomite and calcite to enhance soil health and pak choi growthOriginal Paper

Muhammad Shaaban, Lei Wu, Aneela Younas, Yupeng Wu

Plant Soil Environ., 2025, 71(12):873-882 | DOI: 10.17221/390/2025-PSE

Cadmium (Cd) contamination in agricultural soils threatens crop productivity and food safety. This study examined the use of dolomite and calcite amendments in reducing Cd toxicity in pak choi grown in Cd-contaminated soil. Treatments included: control (CK), Calcite 1 (Cal1, 10 g/kg soil), Calcite 2 (Cal2, 20 g/kg soil), Dolomite 1 (Dol1, 10 g/kg soil), and Dolomite 2 (Dol2, 20 g/kg soil). Amendments significantly increased soil pH (P ≤ 0.05), with Cal2 (6.5) and Dol2 (6.2) achieving the highest values at harvest. Cd availability declined (P ≤ 0.05), with Dol2 being the most effective, reducing the toxicity characteristic leaching procedure-extractable Cd from 0.03 to 0.01 mg/kg, NH4NO3-extractable Cd from 0.05 to 0.02 mg/kg, and CaCl2-extractable Cd from 0.40 to 0.01 mg/kg. Dol2 improved biomass and chlorophyll content, while reducing Cd accumulation in shoots by 73.3% and in roots by 70% relative to the control. Antioxidant enzymes were regulated, with decreased peroxidase and superoxide dismutase indicating reduced oxidative stress, while Dol2 maximised urease, catalase, invertase, phosphatase, and phenol oxidase activities. Dissolved organic carbon and microbial biomass carbon also increased, thereby enhancing microbial activity. Dolomite and calcite significantly reduced biological concentration factors, biological accumulation coefficients, and translocation factors, thereby restricting Cd uptake. Overall, dolomite, especially at higher levels, effectively mitigated Cd toxicity, improved plant resilience, and enhanced soil health in contaminated systems.

Modelling the circular bioeconomy in the palm oil industry: Emerging approaches to address cattle feed shortages in IndonesiaOriginal Paper

Atien Priyanti, Priyono, I Gusti Ayu Putu Mahendri, Dwi Yulistiani, Hotmatua Daulay, Yuhendra, Nasir Rofiq, Astu Unadi, Uning Budiharti, Adji Parikesit, Arsyadi Ali (email: ali_arsyadi

Agric. Econ. - Czech, 2025, 71(12):647-663 | DOI: 10.17221/231/2024-AGRICECON

Indonesia is presently ranked as the first crude palm oil producer in the world. However, the palm oil industry faces significant challenges, including extensive criticism related to environmental degradation and social impacts. The circular bioeconomy concept emphasises sustainable production and consumption through the repurposing, recycling, and regeneration of resources to address these challenges. Integrating palm and cattle farming represents a promising approach to enhancing resource efficiency and sustainability in agricultural systems. This study employs a system dynamics analysis to model the circular bioeconomy in the palm oil industry, with a specific focus on addressing cattle feed shortages. The results demonstrate that utilising oil palm biomass can yield a total of 21 204.52 tonnes of feed and generate a yearly revenue of USD 317 020.14. Key findings indicate that integrating palm by-products into cattle feed not only addresses feed shortages but also reduces waste and enhances overall farm productivity. The implications of this study suggest that adopting circular bioeconomy practices in the palm oil industry can promote more resilient and sustainable agricultural practices.

Closed-circle bioeconomy: Applied aspects of agricultural implementationReview

Yelizaveta Chernysh, Viktoriia Chubur, Miroslav Hájek, Hynek Roubík

Agric. Econ. - Czech, 2025, 71(12):680-690 | DOI: 10.17221/36/2025-AGRICECON

In the face of global challenges, innovative methods of production and consumption are vital, and the bioeconomy signifies a transformational shift based on biological processes, minimal energy use and full integration of resources into ecosystems. This study focused on a sectoral analysis of bioeconomy opportunities with a focus on bioenergy and waste management in an agriculture sustainable approach. Literature-based analysis was used to approach the research objectives of the roadmap design methods. A comprehensive sectoral analysis of the bioeconomy was conducted with a special emphasis on bioenergy and food security. A conditional roadmap of bioeconomy implementation clusters was presented, which is in line with global trends.  Agriculture, being an integral part of the bioeconomy, faces challenges due to ecosystem degradation, water scarcity, and poverty. The global trend of biotechnology adoption in agriculture offers benefits for sustainable bio-economic development, including soil conservation, cost reduction, job creation and improved food quality. Thus, the bioeconomy has significant potential to address global challenges and achieve sustainable development, which requires innovation, regional adaptation and a commitment to harmonise economic growth with environmental conservation. Further research into the involvement of the rural areas in the development of bioeconomy is required.

Mitigating methane in dairy cattle: Integrated strategies and the evolving role of precision livestock farmingReview

Eva Mixtajová, Joana Nery, Radovan Kasarda, Muzaffer Denlí, Achille Schiavone, Alkan Çağlı, José Francisco Pérez, Hasan Hüseyin İpçak, Jo

Czech J. Anim. Sci., 2026, 71(2):41-58 | DOI: 10.17221/116/2025-CJAS


Modern dairy farming faces the dual challenge of meeting global food demands while mitigating its environmental impact, particularly greenhouse gas (GHG) emissions, such as methane (CH4), a potent contributor to climate change. This review explores the role of Precision Livestock Farming (PLF) technologies in monitoring and reducing CH4 emissions from dairy cattle. We evaluate state-of-the-art methods, including direct monitoring (e.g. respiratory chambers, GreenFeed systems) and indirect approaches (e.g. infrared milk spectroscopy, AI-driven analytics), alongside mitigation strategies such as nutritional optimisation, genetic selection, and ruminal additives. PLF emerges as a transformative tool, integrating real-time data on animal health, feed efficiency, and environmental conditions to optimise management practices and reduce emissions per unit of milk produced. By synthesising current research, we highlight the potential of PLF to reconcile productivity with sustainability, offering scalable solutions for the dairy sector. Critical gaps in real-time CH4 monitoring and farm-level implementation are identified, underscoring the need for further innovation. This review provides a roadmap for aligning dairy production with global climate goals while ensuring food security for the growing population.

What drives post-acquisition farm growth? Empirical evidence from UkraineOriginal Paper

Igor Ostapchuk, Taras Gagalyuk

Agric. Econ. - Czech, 2026, 72(3):171-189 | DOI: 10.17221/385/2024-AGRICECON

This study contributes to the limited body of research on mergers and acquisitions in primary agriculture by examining how large corporate agroholdings achieve profitable post-acquisition growth for the farms they acquire. Using System GMM estimation, we analyse farm-level data from 648 farms acquired by agroholdings in Ukraine between 2005 and 2016. Our findings show that agroholdings facilitate post-acquisition growth and performance improvements by employing several integration strategies. Specifically, they leverage horizontally integrated structures to: (i) consolidate land resources of acquired farms to achieve scale economies; (ii) reallocate farm resources toward more profitable production lines; and (iii) intensify production on acquired farms. These results support established firm growth theories, namely, the independence of firm growth rates from firm size (Gibrat's law) and the existence of Penrosean limits to growth.

Genetic parameters for foot and claw disorders in Czech Holstein cattleOriginal Paper

Ludmila Zavadilová, Eva Ka¹ná, Zuzana Krupová, Miloslava ©típková, Michaela Brzáková

Czech J. Anim. Sci., 2026, 71(1):11-20 | DOI: 10.17221/140/2025-CJAS


Our study investigated the genetic variability of specific foot and claw disorders, namely dermatitis digitalis, sole ulcer, and three broader categories (infectious diseases, claw horn lesions, and general claw disorder) in Czech Holstein cows. We also examined their genetic relationships with clinical mastitis and key fertility traits. Using both linear and threshold animal models, we concluded that heritability estimates for foot and claw disorders were consistently higher when employing the threshold model. A significant finding was the genetic correlation between sole ulcer and dermatitis digitalis, which ranged from 0.21 (calving to 305 days in milk) to 0.53 (calving to 90 days in milk). Furthermore, we observed stronger genetic correlations between clinical mastitis and overall foot and claw disorders, claw horn lesions, and infectious diseases during early lactation (calving to 90 days in milk) compared to the entire lactation period. Within this early lactation phase, the strongest genetic correlation was identified between clinical mastitis and claw horn lesions. Interestingly, our research uncovered a unique genetic link between days open and dermatitis digitalis, suggesting that a genetic predisposition to a higher occurrence of dermatitis digitalis may coincide with shorter days open. These results underscore the importance of implementing distinct genetic selection strategies for infectious claw diseases and claw horn lesions in dairy cows. This distinction is crucial due to their differing genetic correlations with other economically important traits, ultimately contributing to a better understanding of claw health genetics and its application in breeding programs.

Nexus of agricultural informatisation and sustainable practices: Food security implications for drought-affected maize farmers in ZambiaOriginal Paper

Shangao Wang, Gershom Endelani Mwalupaso, Xianhui Geng, Emmanuel Kiprop, Jotham Bett, Dancun Kibiwott Kimwei

Agric. Econ. - Czech, 2026, 72(2):101-119 | DOI: 10.17221/84/2025-AGRICECON

Agricultural informatisation (AgI) is hailed as a 'game-changer' for farmers worldwide, even as climate change increases agriculture's vulnerability to climatic risks and threatens sustainable agrifood production. While AgI aspires to help alleviate hunger and poverty in smallholder farm households by improving on-farm productivity through the promotion of sustainable agricultural practices (SAPs), limited empirical evidence exists on the AgI–SAPs nexus, particularly under severe environmental stress such as drought. We analysed data from a survey of maize farmers in central Zambia – a country exemplifying the impact of severe drought, declared a national emergency and disaster – to explore whether and how AgI can optimise SAP adoption and improve crop yields. Given the potential endogeneity of AgI adoption, we employed a recursive bivariate probit (RBP) and endogenous-treatment regression (ETR) to estimate the former and the latter, respectively. We focused on adoption portfolios of three AgI tools – radio, television and mobile phones – and five SAPs: minimum tillage, residue retention, planting basins, improved seed varieties and irrigation. The results reveal that AgI adoption significantly influences SAP adoption, with varying impacts across different AgI and SAP portfolios. Importantly, the adoption of productivity-enhancing SAPs, particularly improved seed and drip irrigation, produced the largest yield effects (124.46 g/capita/day) for AgI adopters. This increase potentially contributes 43.21% towards daily maize-supply quantity, which is crucial for helping households meet the minimum recommended daily caloric intake. The study therefore underscores that AgI plays a critical role in improving yields through SAP adoption, serving as a compelling pathway for agricultural resilience, especially under adverse climatic conditions. These insights align with the United Nations Sustainable Development Goals (SDGs), particularly those aimed at zero hunger, climate action and poverty alleviation, which advocate re-thinking and transforming food-production strategies.

Secondary antibody therapy outperforms corticosteroids in an ameliorating lipopolysaccharide-induced rat model of premature ovarian failureOriginal Paper

AA Zaki, SM Albarrak

Vet Med - Czech, 2026, 71(3):117-128 | DOI: 10.17221/66/2025-VETMED

Premature ovarian failure (POF) is a significant cause of infertility and is often linked to autoimmune aetiologies. Lipopolysaccharide (LPS)-induced inflammation is a well-established model of autoimmune POF in rodents. Immunomodulatory treatments involving corticosteroids, frankincense, and targeted secondary antibodies have been hypothesised to mitigate the autoimmune response, reduce anti-ovarian antibody (AOA) levels, and restore ovarian function in an LPS-induced POF rat model. A POF model was established in female albino rats via the intraperitoneal injection of LPS. The rats were then divided into groups that received no treatment (LPS control), dexamethasone (DEX-treated LPS-treated rats), methylprednisolone (MP-treated LPS-treated rats), frankincense (Frankincense-treated LPS-treated rats), or secondary anti-ovarian antibodies (secondary Ab-treated LPS-treated rats) for 3 to 4 weeks. The serum levels of AOA, 17β-oestradiol, follicle-stimulating hormone (FSH), and luteinising hormone (LH) were assayed via commercial enzyme-linked immunosorbent assay (ELISA) kits. Ovarian tissues were examined histopathologically to assess structural damage and recovery. LPS induction successfully created a POF phenotype, as evidenced by significantly elevated AOA levels (P < 0.001), reduced 17β-oestradiol (P < 0.001), elevated FSH/LH (P < 0.001 and P < 0.05, respectively), and severe histopathological damage, including follicular atresia. All the treatments restored 17β-oestradiol levels. Secondary antibody therapy was most effective, normalising all hormonal parameters, significantly reducing AOA levels, and demonstrating complete histological recovery with healthy follicles and corpora lutea. MP potently suppressed AOA but paradoxically elevated FSH, without improving ovarian histology. DEX and frankincense showed intermediate efficacy, improving some hormonal and serological markers but failing to achieve full histological restoration. These findings demonstrate that targeted immunotherapy using secondary antibodies is superior to broad immunosuppression or anti-inflammatory treatment for restoring ovarian function in patients with autoimmune POF. While corticosteroids effectively reduce AOA titres, they may not reverse ovarian damage and can disrupt the hormonal balance. This underscores the need for precise, biomarker-guided therapies over nonspecific immunosuppression in patients with autoimmune ovarian insufficiency.

Molecular markers and genomic resources in caraway (Carum carvi L.): Current status, research gaps, and strategic directions for breedingReview

Samuel Goitom Misginna, Petra Röszlerová, Omar Gaoua, Patrick Kamulegeya, Marie Pichová, Eva Jozová, Vladislav Èurn

Czech J. Genet. Plant Breed., 2026, 62(3):121-134 | DOI: 10.17221/16/2026-CJGPB

Caraway (Carum carvi L.) is an economically important spice and medicinal crop valued for its essential oil composition, particularly its high content of carvone and limonene. Despite its commercial relevance, compared with that of other Apiaceae species, the development of genomic resources remains limited. Molecular research has progressed from early dominant marker systems, including random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR), to more recent SNP-based genotyping approaches that clarify population structure and flowering-type differentiation. However, key genomic resources, such as simple sequence repeat (SSR) markers, a high-quality reference genome, quantitative trait locus (QTL) mapping, genome-wide association studies (GWAS), and transcriptomic datasets, are still lacking. This review synthesises current knowledge on molecular marker applications in caraway and identifies major gaps limiting breeding progress. Evidence from related Apiaceae species indicates that systematic SSR development and integration of genome-based tools can substantially enhance breeding efficiency. Particular emphasis is placed on a phased strategy for SSR development in caraway, positioned as complementary to single-nucleotide polymorphism (SNP)-based approaches within a progressive genome-enabled breeding framework. Strengthening the molecular infrastructure of caraway will support precision breeding aimed at improving yield stability, essential oil quality, and environmental adaptability.

Drought-induced metabolic adjustments in woodland strawberry leaves: the role of soluble carbohydrates and starchOriginal Paper

Jaromír Hamet, Hana Konrádová, Helena Lipavská

Plant Soil Environ., 2026, 72(2):87-101 | DOI: 10.17221/561/2025-PSE

Water deficit is one of the most important abiotic factors limiting crop yields. To better understand the link between carbohydrate balance and drought stress response in strawberry plants (Fragaria vesca), we monitored by liquid chromatography the carbohydrate status in leaves during exposure to drought of different duration and intensity as well as subsequent recovery. In two greenhouse experiments that differed in the rate of reaching the target water deficit, strawberry leaves showed osmotic adjustment, with gradual increases in glucose and fructose content, likely provided by observed starch degradation. At the point of the most severe stress, proline content increased, while stress markers, such as malondialdehyde content and chlorophyll fluorescence, showed no significant changes. It indicates the defence mechanisms’ ability to protect cellular structures effectively. Strawberry, a member of the Rosaceae family, motivated us to investigate the role of sorbitol in the stress response. However, we found no sorbitol in any stress or control situations. Finally, testing sorbitol’s ability to support strawberry plant or non-green callus growth in vitro did not indicate that sorbitol could be used as a carbon and energy source. In conclusion, strawberries exhibit marked changes in soluble carbohydrate and starch content as an efficient defence against drought, without apparent involvement of sorbitol.

Impact of hemp (Cannabis sativa L.) variety on the seed and stem yield, biochemical characteristics of the inflorescences and nutritional quality of seedsOriginal Paper

Marko Flaj¹man, Anita Ku¹ar, Helena Abramoviè, Jerneja Jakopiè, Darja Kocjan Aèko, Barbara Èeh

Plant Soil Environ., 2026, 72(2):122-137 | DOI: 10.17221/556/2025-PSE

Hemp is becoming increasingly popular, and many new varieties are coming onto the market to meet the requirements of different industries. In this study, the seed and stem yield, seed nutritional properties and the biochemical characteristics of the inflorescences of seven European varieties (Fedora 17, Futura 75, KC Dóra, Monoica, Santhica 27, Tiborszallasi, USO 31) were investigated in a 3-year field trial. Futura 75 and Tiborszallasi stand out as varieties with the highest potential in the conditions of the experiment (humid continental climate with oceanic influences, heavy soil). Futura 75 achieved the highest seed yield (505 kg/ha dry matter), stem yield (8 036 kg/ha fresh matter), protein yield (140 kg/ha) and oil yield (181 kg/ha). There were no differences in protein content (average 21.0%) among varieties. The total unsaturated fatty acid content was as high as 87.6% at Tiborszallasi. The best ratio between omega-6 and omega-3 fatty acids was 3 : 1 in Tiborszallasi, which had also the highest oil content (30.2%), the highest total phenolic content (2.8 mg caffeic acid (CA)/g) and the best antioxidant potential (6.69 EC50 DPPH (2,2-diphenyl-1-picrylhydrazyl) mg/L). Most varieties had higher cannabidiol and tetrahydrocannabinol contents in the inflorescence at seed maturity (from 0.22 to 3.3 for cannabidiol (CBD) and from 0.00 to 0.32 for tetrahydrocannabinol (THC)) compared to full flowering (from 0.17 to 4.33 for CBD and from 0.00 to 0.52 for THC, on average 2.64% for CBD and 0.19% for THC), presenting an opportunity for dual-purpose use.

Hormetic growth of Pinus pseudostrobus seedlings exposed to low-dose gamma and X-ray irradiationOriginal Paper

Laura Yasmin Flores López, César Valenzuela Encinas, Eulogio De la Cruz Torres, María de Lourdes Palafox Chávez, Ivar Diego Aceves Sánchez, Lourdes Georgina Iglesias Andreu

J. For. Sci., 2026, 72(3):107-118 | DOI: 10.17221/83/2025-JFS

Mexico ranks among the countries with the highest deforestation rates, increasing the demand for high-quality forest seedlings of valuable species such as Pinus pseudostrobus Lindl., which face germination limitations due to seed dormancy and low viability. Ionising radiation has emerged as an alternative pregermination treatment capable of inducing adaptive responses in plants through hormesis. This study evaluated the effects of different doses of gamma radiation (60Co) and high-energy X-rays (linear accelerator, 6 MeV) on the germination, growth, and quality of P. pseudostrobus seedlings. A total of 1 440 seeds were irradiated per radiation source with 12 doses (0–25 Gy) and sown under nursery conditions in a completely randomised design. Germination parameters, morphological traits, photosynthetic pigment content, and quality indices were analysed. With both radiation sources, low doses (0.5–1.5 Gy) significantly enhanced germination, chlorophyll content, and seedling height and diameter, while doses above 15 Gy inhibited these responses. The LD50 (median lethal dose) was estimated at 20 Gy for gamma rays and 12 Gy for X-rays, whereas GR50 (median growth reduction dose) exceeded 45 Gy in both treatments. These findings demonstrate that low radiation doses elicit a beneficial hormetic effect in P. pseudostrobus, representing a viable biotechnological approach to improve seedling production and ecological reforestation efficiency.

Degradation of Betula spp. under the influence of biotic factors in the forests of Ukrainian PolissiaOriginal Paper

Ivanna Kulbanska, Maryna Shvets, Svitlana Matkovska, Tetiana Melnyk, Vasyl Zayachuk, Nataliya Horbenko, Oleh Zymaroiev, Anastasiia Zymaroieva

J. For. Sci., 2026, 72(3):148-160 | DOI: 10.17221/96/2025-JFS


The paper examines the impact of biotic factors – mainly pests and pathogens – on the degradation of Betula spp. in the forests of Ukrainian Polissia. The taxonomic structure of the identified mycobiota includes representatives of 9 genera, 8 families, 5 orders, 4 classes, and two divisions (Ascomycota 44.4%, Basidiomycota 55.6%). Xylotrophic Basidiomycetes, particularly Fomitopsis betulina and Fomes fomentarius, pose the greatest threat due to their ability to destroy wood tissues. The bacterial pathogen Lelliottia nimipressuralis, the agent of wetwood, also plays a major role, impairing xylem water transport and causing systemic physiological imbalance in affected trees. Entomofauna significantly contributes to degradation processes. A total of 31 insect species were recorded, belonging to five orders, 24 families, and 29 genera, with Coleoptera being the most numerous (32.3%). The most harmful are xylophagous species (e.g. Agrilus betuleti, Rhagium mordax) and phytophagous species (e.g. Geometra papilionaria, Parornix betulae), which cause mechanical tissue damage and facilitate secondary infections. The results indicate that pathogenic complexes intensify under environmental stress, accelerating the decline of Betula spp. stands. These findings underscore the necessity for ongoing phytosanitary monitoring and adaptive management measures to mitigate further degradation risks in the birch forests of Polissia.

Effects of foliar application of potassium dihydrogen phosphate on the physiological responses of rice seedlings under high temperature stressOriginal Paper

Hui Xu, Lei Wang, Dongyue Sun, Wei Liu, Shuhua Jiang, Lijun Zhou, Lu Tang, Xin Gu, Muhammad Ahmad Hassan

Plant Soil Environ., 2026, 72(6):347-361 | DOI: 10.17221/168/2026-PSE

This study investigated the alleviating effects and physiological responses to foliar-applied potassium dihydrogen phosphate (KDP) on rice seedlings under high-temperature (HT) stress. An early indica hybrid rice, YLY17 (high-temperature-sensitive), was used as the planting material. Four treatment groups were set up: (a) NT – normal temperature; (b) NT + KDP – normal temperature with foliar application of different KDP concentrations (0.1, 0.2, 0.3, and 0.4%); (c) HT – high temperature treatment without foliar application of KDP, and (d) HT + KDP – high temperature with foliar application of different KDP concentrations. At the three-leaf stage, rice seedlings were subjected to simulated HT stress (32~38 °C during the day and 26~32 °C at night) for 10 days. Growth indicators, photosynthetic parameters, antioxidant characteristics, osmotic adjustment substances, and related metabolic enzymatic activities of young rice seedlings were quantified, and the alleviating effect of KDP was comprehensively evaluated by principal component analysis (PCA). The results showed that HT stress significantly reduced plant height, fresh weight, and dry weight, decreased chlorophyll content and SPAD value, and decreased the net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr), while increasing intercellular carbon dioxide (CO2) concentration (ci). At the same time, it led to the accumulation of superoxide anion (O2), hydrogen peroxide (H2O2), and malondialdehyde (MDA), and induced increases in antioxidant enzyme and osmotic adjustment-related enzyme activities. Foliar spraying of KDP could effectively alleviate the above damage caused by HT stress, with 0.3% KDP being the most effective treatment. Compared with HT treatment, 0.3% KDP treatment significantly increased plant height, fresh weight and dry weight by 7.6, 10.6 and 10.2%, respectively, improved chlorophyll content and photosynthetic parameters, enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), reduced the accumulation of reactive oxygen species (ROS) and MDA, and promoted the accumulation of osmotic adjustment substances such as soluble protein (SP), proline (Pro), soluble sugar (SS) and sucrose (SUC), as well as increased the activities of nitrate reductase (NR), glutamine synthetase (GS), sucrose synthase (SUS) and sucrose phosphate synthase (SPS). The PCA results showed that the order of comprehensive physiological activity index was NT + KDP > NT > KDP + HT > HT, indicating that KDP enhances heat tolerance by coordinately regulating photosynthesis, antioxidant defence, and osmotic adjustments. This study provides a theoretical basis and technical reference for using KDP to alleviate HT stress in rice seedlings.

Hydrological risks of clear-cuts after the bark beetle outbreaks and related forest management decisions in Central EuropeReview

Vít ©rámek, Kateøina Neudertová Hellebrandová, Vìra Fadrhonsová

J. For. Sci., 2026, 72(4):161-173 | DOI: 10.17221/19/2026-JFS


The review synthesises current knowledge on the hydrological and hydrochemical risks associated with large-scale clear-cutting following unprecedented bark beetle outbreaks in Central Europe. By analysing 107 sources published primarily between 2000 and 2026, we evaluate the divergent impacts of natural forest dieback versus intensive salvage logging. The rapid loss of Norway spruce [Picea abies (L.) Karst.] canopy has fundamentally altered the microclimate, shifting the energy balance from latent to sensible heat flux, which results in ground temperature increases of up to 5.2 °C. Hydrological consequences include a 16–48% reduction in interception and a cessation of transpiration, leading to an increase in annual water yield by 6–21% and potentially accelerated peak discharges, particularly in headwater catchments where maximum flows can increase by over 50%. The synthesis highlights a critical 'nitrate pulse' exceeding 50 mg·L–1 in managed areas, contrasting with higher hydrochemical resilience in unmanaged stands. We emphasise that forest management must ensure sufficient soil protection during the logging and wood transportation, which can reduce hydraulic conductivity by over 40%. The role of logging residues is important because they cover and protect the soil environment. The study concludes that utilising pioneer species and spontaneous succession in combination with timely artificial reforestation represents a superior strategy for stabilising the microclimate and restoring hydrological functions.

Optimisation of agricultural logistics: A systematic review of modelling techniques and economic potentialsReview

Nikolas Bublik, Sebastian Hess

Agric. Econ. - Czech, 2026, 72(4):253-271 | DOI: 10.17221/76/2025-AGRICECON

Agricultural logistics face unique challenges such as seasonal demand fluctuations, perishability, and geographic dispersion. The paper systematically analyses 63 peer-reviewed articles from 2013 to 2025, focusing on key optimisation techniques, including multi-criteria decision-making (MCDM), vehicle routing problems (VRP), and path planning problems (PPP). The findings highlight how logistics optimisation can reduce operational costs, improve resource utilisation, and enhance supply chain resilience. Additionally, the study identifies gaps in inbound logistics research and emphasises the need for further integration of digital technologies. Future research should focus on comprehensive, technology-driven solutions to improve adaptability and transparency in agricultural supply chains. Key findings reveal that optimised logistics models can lead to cost reductions of up to 58%, emissions savings of over 60%, and significant improvements in delivery time, field efficiency, and customer satisfaction.

Occurrence of Norovirus genogroup II in leafy greens in the region of Marrakech using a molecular methodOriginal Paper

Salma Berrouch, Nouhaila El Fellaki, Abdelkader Biary, Hibatallah Lachkar, Houda Rafi, Halima Rouane, Simeon Goïta, Jamal Eddine Hafid

Czech J. Food Sci., 2026, 44(2):123-131 | DOI: 10.17221/101/2025-CJFS

Leafy greens are important vectors for enteric viruses, including human noroviruses (HuNoV), which are a leading cause of foodborne disease. These viruses can contaminate the agricultural environment through untreated wastewater or direct contamination. While studies on HuNoV in vegetables have been conducted, few have focused on Morocco. This study aimed to detect HuNoV in leafy greens collected in the region of Marrakech region over a fifteen-week period (March–June 2023). For this purpose, 112 samples (coriander, lettuce and parsley) were collected and analysed using the ISO 15216-2:2019 method with minor modifications, after validation. The method involved eluting viruses using an alkaline buffer, concentrating them through polyethylene glycol precipitation, and detecting viral RNA via real-time RT-PCR. The applied method yielded variable recovery rates among the tested matrices, with coriander showing the highest recovery (1.5%), followed by lettuce (1.2%) and parsley (0.6%), confirming a significant matrix-dependent variation in HuNoV recovery (ANOVA, P < 0.001). This method enabled the assessment of leafy green contamination, which was found to be 0.89% (1/112). This study underscores the need to enhance detection methods to better assess the risks associated to noroviruses in leafy greens, with implications for human health.

A systematic review on the modulation of heat shock protein 70 in broilers at different stages of growth under thermal stressReview

Hudu Ramalan Abdullahi, Abubakar Abubakar Ahmed, Suriya Kumari Ramiah, Hasliza Abu Hassim, Yong Meng Goh

Czech J. Anim. Sci., 2026, 71(4):141-158 | DOI: 10.17221/1/2026-CJAS


This systematic review synthesises evidence from published articles investigating nutritional strategies to modulate heat shock protein 70 (HSP70). A total of 1 616 records were identified from four databases and snowballing. After screening and eligibility assessment, 25 studies comprising 29 reports published between 2004 and 2025 were included. The analysis shows that targeted interventions particularly antioxidants (vitamins C, E, and selenium), amino acids and their derivatives (methionine, betaine), and phytogenic compounds effectively downregulate HSP70 expression in a dose or tissue-dependent manner. This modulation is associated with improved growth performance, enhanced redox balance, and normalised stress hormone profiles. Key findings indicate that synergistic combinations outperform single additives in suppressing HSP70 and improving carcass yield under chronic heat stress (32–38 °C); the early-life nutrient delivery modulates HSP70 expression and influences post-hatch thermotolerance; and organic mineral or methionine hydroxy analogue are more effective than inorganic forms. However, efficacy varies by life stage, with most studies focused on Ross and Cobb genotypes, and is influenced by gut microbiota interactions. Major research gaps remain, including defining stage-specific optimal HSP70 thresholds, clarifying links between HSP70 modulation, immunity or gut health, understanding long-term effects of early-life nutritional programming, and determining the influence of administration routes on nutrient efficacy.

A global meta-analysis of fertiliser management on soil available and total zincOriginal Paper

Junyan Ren, Waseem Hassan, Qindi Zhang, Andong Cai

Plant Soil Environ., 2026, 72(5):321-337 | DOI: 10.17221/23/2026-PSE

Soil available and total zinc are important indicators of soil zinc status, yet the global effects of different fertilisation practices on soil available and total zinc and the drivers of their variation remain insufficiently quantified. We conducted a global meta-analysis based on data extracted from published field studies. A total of 1 240 paired observations of soil available zinc from 94 studies and 364 paired observations of soil total zinc from 44 studies published between 1993 and 2024 were compiled. The effects of eight fertiliser types (mineral fertilisers without zinc [CF], compost, manure, zinc fertiliser, CF combined with either compost [CFC] or manure [CFM] or zinc fertiliser [CFZn], and compost combined with zinc fertiliser [CZn]) on the soil available and total zinc content were assessed by meta-analysis. The results indicated that compared to the control group, soil available zinc content increased significantly under treatments CZn, CFZn, zinc fertiliser, CFM, manure, CFC, and compost by 158, 134, 133, 84, 78, 43, and 35%, respectively. Additionally, manure, CFM, zinc fertiliser, CFZn, and CZn treatments significantly enhanced soil total zinc content, with increases ranging from 25% to 32%. Applying zinc fertiliser at > 20 kg Zn/ha significantly increased soil-available zinc. In the medium-rate CZn class (10–20 kg Zn/ha), soil available zinc increased from 0.78 mg/kg in the control soils to 3.46 mg/kg in the treated soils. Among crop systems, wheat showed a stronger response in soil-available zinc, whereas rice-growing systems showed relatively larger increases in soil-total zinc under manure and CFM treatments. Fertilisation intensity, crop types, soil organic carbon, and soil pH emerged as key drivers of variation in soil available zinc, whereas the main drivers of soil total zinc varied among fertiliser types and were more often associated with fertiliser rate and crop types. When soil organic carbon was ≤ 12 g/kg or soil pH was > 7.5, applying CZn at 10–20 kg Zn/ha showed greater potential to increase soil available zinc. These findings suggest that soil zinc management should be optimised based on fertilisation intensity, crop type, soil organic carbon, and soil pH to improve zinc availability while avoiding excessive accumulation.

The effect and dynamic transmission mechanism of African swine fever on pork prices in China: A study based on the staggered DID model and SVAR modelOriginal Paper

Yi Li, Yu Wang, Liling Zhu, Beining Yan

Agric. Econ. - Czech, 2026, 72(5):314-339 | DOI: 10.17221/350/2024-AGRICECON

African swine fever (ASF) has spread rapidly, substantially disrupting the pork market. In this study, we treat the 2018 ASF outbreak in China as a quasi-natural experiment, using staggered difference-in-differences (DID) and spatial DID methods to assess its effect on pork prices. We use a structural vector autoregression model to identify the sources of price fluctuations. In the study, we also explore the mechanisms and heterogeneity in the effects of ASF on pork prices. The results show that (i) ASF significantly raises pork prices across various provinces; (ii) live pig prices are positively correlated with finishing pig feed prices, piglet prices and pork prices in the long term but negatively correlated with the ASF index; (iii) the key drivers of live pig price fluctuations include price inertia (59%), finishing pig feed prices (13%), piglet prices (11.5%), pork prices (10.8%) and the ASF index (5.6%); (iv) for pork prices, the largest driver is live pig prices (53.4%), followed by finishing pig feed prices (14%), piglet prices (13.6%), price inertia (13.4%) and the ASF index (5.6%); (v) mechanism analysis reveals that ASF affects pork price fluctuations through farming costs and wholesale-retail profits. The heterogeneity analysis results reveal that provinces with higher internet information levels, weaker agricultural development and those in the eastern region are more vulnerable to the effects of ASF. On the basis of these findings, we offer targeted policy recommendations.

Investigating the role of 6-BAP and 2,4-DPA in enhancing biochemical nutrients and reducing fruit drop and cracking of litchi cv. BombaiOriginal Paper

Bikash Chandra Sarker, Shams Shaila Islam, Kanij Farhana, M.A. Hakim, Thanet Khomphet

Hort. Sci. (Prague), 2026, 53(2):148-159 | DOI: 10.17221/47/2025-HORTSCI

Litchi fruit dropping is a major issue that leads to low yields while degrading biochemical nutrient qualities by fruit cracking and reduced income for litchi growers in Bangladesh. To address this, an experiment was conducted to determine the effects of biochemical nutrients and the ability to reduce fruit cracking and dropping in the litchi cv. ‘Bombai’ under various concentrations of 6-benzylaminopurine (6-BAP) and 2,4-dichlorophenoxyacetic acid (2,4-DPA). The study followed a two-factor factorial experiment in a randomised complete block design, using four levels for each growth regulator: control, 20, 30, and 40 ppm 2,4-DPA, and similarly for 6-BAP. Various parameters were measured, including the number of flowers, fruit retention, fruit cracking, moisture content, ash content, acidity, fruit growth, and fruit quality, as well as yield per tree. The results demonstrated that both plant growth regulators, 2,4-DPA and 6-BAP, significantly reduced fruit dropping and cracking while improving the overall quality and nutrient content of the fruits. The retention of fruits was higher with 6-BAP compared to 2,4-DPA. The highest fruit weight, length, diameter, pulp-to-seed ratio, and pulp-to-peel ratio were achieved with the 20 ppm treatments of both 2,4-DPA and 6-BAP. Additionally, biochemical fruit quality indicators, including vitamin C, vitamin A, total phenol content, and key mineral nutrients, were significantly enhanced by both growth regulators. Based on the findings, it is recommended that either 20 ppm of 2,4-DPA or 20 ppm of 6-BAP be applied three times at the pea-size, marble-size, and pre-harvest stages after fruit set to enhance fruit quality by reducing fruit crack and reducing fruit drop resulted in higher yield in litchi.

The Auto Sprinkler Rover: An innovative fertiliser applicator for sustainable agricultureOriginal Paper

Wan Khaima Azira Wan mat khalir, Azimah Ismail, Nur Maizatul Akma Abdul Aziz

Res. Agr. Eng., 2026, 72(2):95-108 | DOI: 10.17221/165/2025-RAE


Fertilisers are essential to agriculture since they provide crucial nutrients that stimulate crop growth, increase the yield and improve the yield quality. However, this practice requires the use of manual labour, which consumes energy and time as well as limits the effectiveness of fertilisation methods and is environmentally harmful. These factors have led to the development of an innovative product based on the Internet of Things (IoT) called the Auto Sprinkler Rover, which is a remote-controlled machine that operates automatically. It contains a 12 L water barrel that serves as a storage tank for a liquid fertiliser. This study designed a liquid sensor and light emitting diode to alert th–e user to the amount of liquid in the tank. The water pump facilitates the smooth spraying of liquid fertiliser from the pipe's nozzle. The Auto Sprinkler Rover differs from conventional methods because it integrates real-time monitoring via Wi-Fi, an automatic liquid level sensor as well as an automatic spraying system and an ergonomic design that is manually operated. The Auto Sprinkler Rover was designed to be a cutting-edge technology that benefits sustainable crop cultivation for households, communities and the global agriculture industry.

Investigation of the drying kinetics of a residual nut mixture using mathematical models and artificialneural networksOriginal Paper

Tran Huu Duy, Nguyen Doan Kim Dang, Dang Nguyen Gia Han, Pham Tran Thanh Vy, Tran Ngoc Giau, Hong Van Hao, Nguyen Minh Thuy, Vo Quang Minh, Ngo Van Tai

Res. Agr. Eng., 2026, 72(2):121-131 | DOI: 10.17221/218/2025-RAE

The objective of this study was to evaluate the potential for the sustainable reuse and the value of the residual nut mixture (RNM) by-products (cashew nut, peanut, and soybean) after extraction. To investigate the drying kinetics, the RNM was dried at various temperatures (50 to 80 °C). The Balbay and ªahin model, which had a high coefficient of determination (R2) of 99.62–99.96%, a low root mean square error of 0.007–0.021, and χ2 of 0.001–0.005, was the one that best fit the experimental data out of the eight mathematical models that were used. Artificial neural networks showed higher and faster prediction capacity than the mathematical models. The effective moisture diffusion coefficient (Deff) increased gradually with the temperature, with an activation energy (Ea) of 13.67 kJ∙mol–1. The RNM powder produced by the optimal drying process (60 °C for 3.75 h) has a bright colour, high polyphenol content (2.68 mg gallic acid equivalent (GAE)∙g–1) and antioxidant activity, low moisture content (4.9%) and relatively high nutritional value, especially protein (27.27%), lipid (40.19%), and fibre (4.2%). Under these conditions, not only is efficient drying and preservation achieved, but the quality of the by-product powder is also maintained.

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