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Hepatic lipidosis and ketosis are frequent metabolic disorders in dairy cows during the transition period. The 2-methyl-2-phenoxy propionic acid (MFPA) is an activator of energy metabolism. The objective of this study was to evaluate the effect of administration of MFPA 10 days prepartum to day 36 postpartum on serum lipid profile, ovarian reactivity, and milk yield. Fifty seven multiparous Holstein cows were divided into four groups. The groups 1 (n = 14) and 2 (n = 14) had body condition score (BCS) above 4; BCS of the groups 3 (n = 15) and 4 (n = 14) was between 3.25 and 3.75 at calving. The solution containing 10 mg/kg MFPA was injected intramuscularly (IM) to cows of groups 1 and 3, 10-7 days prepartum and 6 h postpartum. The groups 2 and 4 received 50 ml of 0.9% NaCl as placebo in the same way. Blood samples for serum lipid profile were collected from all cows 10 days before calving without treatment, 2 days after the first administration of MFPA and postpartum at days 2, 10, and 21 after the second administration of MFPA. At days 21, 24, 27, 30, 33, and 36 postpartum, blood samples were obtained for plasma progesterone determination. Milk yield was registered daily during 60 days postpartum. Prepartum free fatty acids (FFA) were mildly increased without difference among all the groups (P > 0.05). b-hydroxybutyrate (BHB) was increased only at day 10 after calving in cows of group 1 treated with MFPA (P < 0.05) due to higher number of postpartum diseases. In cows from groups 3 and 4 (BCS normal), BHB was mildly increased, not indicating subclinical ketosis. The serum concentrations of triacylgycerols, cholesterol, albumin, activities of aspartate aminotransferase and creatine kinase were within the limits of reference values, indicating adequate liver integrity and function. Cows of normal BCS treated with MFPA presented better milk production but without significant difference among four groups (P > 0.05). Ovarian reactivity was present at day 21 in all groups of cows without difference (P > 0.05). The administration of MFPA had no effect on serum lipid profile and ovarian activity in this study.

Field experiments were conducted to determine the effects of field bindweed and powdery mildew on growth and yield of a pepper crop. This study also evaluated the efficacy of fungicide application programs for controlling powdery mildew. Field bindweed influenced growth and yield of pepper. The greatest dry weight and yield of pepper were recorded with weed-free control treatment. Moreover, powdery mildew was severe. The fungicide application programs positively influenced growth and yield of pepper. The control plots had the lowest yield of pepper. The first symptoms of powdery mildew on pepper plants developed 20-25 days after powdery mildew diagnosis on field bindweed. Two azoxystrobin applications, at 10 and 25 days after infection of field bindweed by powdery mildew, restricted the disease progress. Our results indicate that field bindweed is highly susceptible to powdery mildew infection and could be used as an indicator species of L. taurica presence in pepper plants.

Comprehensive analyses of soil properties of sites of native Scandinavian broadleaved tree species were performed in 36 habitats in East Norway. The material consisted of stands of silver birch (Betula pendula Roth.), white birch (Betula pubescens Ehrh.), black alder (Alnus glutinosa Gaertn.), speckled alder (Alnus incana Moench.), European ash (Fraxinus excelsior L.), pedunculate oak (Quercus robur L.) and sessile oak (Quercus petraea [Matt.] Liebl.). The main objective was to describe the vertical characteristics and variations in some selected soil variables of the soil profiles. Particular soil horizons of 15 Brunisolic soils, 11 Regosolic soils, 6 Gleysolic and 4 Podzolic were sampled and analyzed for soil texture, bulk density, specific density, porosity, oxidizable carbon, total nitrogen content, pH in water, exchangeable acidity, exchangeable cations and anions (Mg, Ca, Mn, Al, S, Fe, B, P and K), cation exchange capacity and base saturation. No regular patterns were found in selected soil properties when tested between various soil units in silver birch stands. Furthermore, silver birch stands were found on sites, which topsoil (i) significantly differed in their cation exchange capacities, (ii) did not differ significantly in their pH values, and (iii) mostly differed in their clay contents and (iv) mostly did not differ in BS. Differences among the Humic Regosols, Luvic Gleysols, Sombric Brunisols, Eutric Brunisols and Humo-Ferric Podzols for silver birch stands in their topmost horizons of humified organic matter intimately mixed with the mineral fraction horizons and differences among particular soil horizons for the main soil properties under all the selected broadleaved tree species stands are discussed.

The Lusatian lignite-mining district (Eastern Germany) is characterized by a high share of marginal post-mining areas. At these sites, crop yield is generally low, and hence, conventional land use systems often fail in terms of reliable and efficient crop production. In this paper the attempt is made to evaluate the production of woody biomass for bioenergy in short rotation coppices (SRC) and alley cropping systems (ACS) with black locust (Robinia pseudoacacia L.) and lucerne (Medicago sativa L.) from the aspect of possible ecological and economic benefits compared to the conventional agricultural recultivation practice. The results show that, due to both high establishment and harvesting costs and comparatively low prices of energy wood, land use systems such as SRC or ACS are currently hardly profitable compared to conventional agriculture. However, the cultivation of black locust resulted in a higher humus accumulation and in a lower harvest-related nutrient export than the cultivation of lucerne as a typical recultivation crop in this region. Therefore, it can be concluded that for an improvement of soil fertility woody biomass production is more beneficial than the conventional agricultural recultivation practice.

The aims of the paper were as follows: (1) to assess the applicability of speckled alder for pioneer stands on the mountainous clear-cut tracts and (2) to evaluate the effects of slow-release fertilizer on the survival and growth of speckled alder under harsh environmental conditions. On the basis of seven-year results we can presume that speckled alder is a convenient pioneer species that can be cultivated on harsh mountain sites where the climatic extremes need to be alleviated and a layer of surface humus renewed. The initial slow-release fertilization is, nonetheless, highly desirable. As contrasted to the control the surface and planting hole applications of amendment reduced the total seven-year mortality rate by 9.5% and 20.1%, respectively, and the periodic annual height increment (2003-2009) was promoted by 47% and 59%, respectively. Analogous results were obtained when the values of basal stem diameter and crown diameter were compared. As for the method of application, the placement of the slow-release amendment in the planting holes seems to be more efficient mainly in terms of survival promoting. The surface application is, nonetheless, less laborious and also yielded satisfactory results.

This paper presents research results on the performance of the Timberjack 450C skidder in timber skidding at two working sites of broadleaved trees in mountainous conditions in the Hyrcanian forest. To evaluate the current skidding system in the Hyrcanian forest in northern Iran and possibility of finding out better techniques and group organization an empirical time study has been conducted. The elements of the skidding work phase were identified and 123 cycles were recorded for short-log and long-log method. The models for effective time consumption, total productivity and unit cost of skidding in short-log and long-log method were calculated. The time consumption and productivity of skidding depend on several variables such as distances and slope, number of logs per cycle and volume. The average load per cycle in short-log and long-log method was 2.77 m³ and 3.08 m³, the average one-way skidding distance was 380 and 497 m, the average slope was 18 and 20% in the short-log and long-log method, respectively. The average travel speeds of unloaded skidder were 5.74 km.h^-1 and the average speeds of loaded skidder were 7.67 and 6.16 km.h^-1 in short-log and long-log method, respectively. The average speeds of pulling the cable were 1.71 km.h^-1, and of load winching 0.72 km.h^-1 and 0.69 km.h^-1. The average outputs in short-log and long-log method were 10.86, 11.11 m³.effective h^-1. Results indicated that hourly costs of operation were higher for the short-log method than for the long-log method (12.69 vs.12.40 USD.m^-3).

Among other important factors, vegetation cover strongly affects the hydrological processes in mountain catchments. In this paper, we present the results of field infiltration measurements at the location of various vegetation covers, together with an estimation of the infiltration characteristics of a small mountain forest catchment. Measured steady infiltration rate values were extrapolated on the basis of the dominant plant species distribution in the catchment. We determined which plant species are dominant, and infiltration tests were carried out where these species were located in selected sites in the catchment. The characteristic steady infiltration rates were averaged for each dominant vegetation species. The percentages of dominant plant species were determined for established points placed in a regular network throughout the basin. An extrapolation of the directly measured infiltration values to these established points was calculated using (1) percentages of the dominant plant species determined at these points, and (2) characteristic infiltration rates averaged for these species. An infiltration map was created from the infiltration values calculated for the established points.

The effects of storage on the lipid composition of three redgram (TTB-7, BRG-1, and ICP-8863) and three greengram (PUSA BISAKI, KDM-1, and CHINA MOONG) varieties were studied. Environment variables such as temperature and relative humidity were recorded during storage period. Moisture content was found to increase along with the percent incidence of storage insects. The incidence of storage moulds and insects was recorded in all the samples; results showed that storage moulds belonged to Aspergillus species and the insect Callosobruchus chinensis infested the stored samples. The total fat, triglycerides, phospholipids, free fatty acids and peroxide value were evaluated in the control and in samples stored for three and six months. Results showed that storage depleted total fat (1.94-1.75 g), triglycerides (1.46-1.07 g), whereas phospholipids (0.06-0.21g), free fatty acids (0.002-0.01 g) and peroxide values (2.14-4.46 meq) increased. The fatty acid content of palmitic (26.03-23.56%), stearic (7.4-5.46%), linoleic (56.2-45.2%) and linolenic acids (6.9-4.7%) decreased, but oleic acid content increased (8.3-21.6%) in all the varieties during storage.

The CULTAN (Controlled Uptake Long Term Ammonium Nutrition) system is based on one-time injection of the whole dose of nitrogen required for the vegetation period. The effect of this method on yield and grain quality of winter wheat was observed in a 2-year small-plot trial at 4 different experimental sites in the Czech Republic. The experiment comprised two treatments with the total amount of nitrogen applied during fertilization of 150 kg N/ha. At the CULTAN treatment the whole dose was applied all at once using the GFI 3A injection machine (Maschinen und Antriebstechnik GmbH Güstrow), whereas at the control treatment, the dose was divided into three applications. The average grain yield of winter wheat in 2007 was 9.56 t/ha (control) and 8.78 t/ha (CULTAN); in 2008 it was 9.91 t/ha (control) and 9.63 t/ha (CULTAN). The differences in 2008 were not statistically significant. The contents of nitrogen and gluten were significantly lower at CULTAN treatment in both years. The values of falling number, Zeleny test and bulk density were generally similar at both treatments.

Road salting is used as a dominant way to keep road safety in winter, even in the protected natural areas. In our study, possible effects of winter road salting on soil microorganisms in close road vicinity were investigated. Soil chemical and microbial properties were monitored at a forest site in the Krkonoše Mountains national park and at a grassland site in the Kokořínsko protected landscape area (both located in the Czech Republic) in two sampling campaigns (autumn and spring). Effects of road salting on soil chemical properties (Na⁺ and Cl^- levels, pH, base saturation etc.) were clearly apparent at both sites. The most affected plots were 1 and 5 m from the road (increased pH, base saturation, and Na⁺ accumulation). At these plots, changes of microbial parameters were observed in both autumn and spring sampling, which suggested influence of salts. Increased value of metabolic quotient (qCO₂) indicated stress and potential ammonification was inhibited even 5 m from the road at the forest site. Hence, possible influence on soil biological quality should be considered when assessing the ecological risks of this kind of road treatment, especially in natural protected areas.

The establishment of plants with high damage tolerance may provide a means for soil protection on sites exposed to strong disturbance. In a pot experiment, we investigated the tolerance to mechanical strain of ten grassland plant species representing three growth form groups (cespitose: Festuca arundinacea, Lolium perenne, Taraxacum officinale; rhizomatous: Achillea millefolium, Elymus repens, Poa pratensis; stoloniferous: Agrostis stolonifera, Festuca rubra rubra, Poa supina, Trifolium repens). We hypothesised that growth form and pre-disturbance biomass allocation to the root serve as predictors of damage tolerance. With a tool imitating the action of cleated football boots or scratching chicken, we applied three standardized levels (moderate, medium, strong) of a torsional force which exceeded the shear strength of the sward and impacted on shoots and roots. Post-treatment shoot biomass in relation to shoot biomass of the non-treated control plants served as a measure of damage tolerance. Species, but not growth form groups, differed significantly in damage tolerance, with F. arundinacea and P. pratensis showing the best performance. Shoot re-growth was strongly correlated with relative post-treatment root biomass across all species and treatment levels (R² = 0.25, P < 0.001), but not with pre-treatment root biomass. We conclude that root resistance to mechanical damage is the prevalent determinant of tolerance to disturbance.

This study aims to reveal and to compare effect of two various systems of nitrogen nutrition, namely injection and sidedress application, on the levels of NO3- and phytohormones (cytokinins, abscisic acid (ABA), indole-3-acetic acid (IAA)) in Festulolium plants. Calcium nitrate (CN) or ammonium sulphate (AS) were used as N source in the pot experiment. The yield of Festulolium above and below ground biomass was more substantially enhanced after sidedress application of both AS or CN in comparison with injection application. The levels of endogenous cytokinins peaked 5 days after application regardless the form of N source and the method of application. The increased concentration of endogenous cytokinins as well as the enhanced content of selected free amino acid suggest that N applied sidedress can maintain better distribution patterns of nutrients and provide improved conditions for growth and nitrogen uptake. The nitrogen application affected ABA concentrations on all tested treatments. The correlation between NO3- and ABA concentrations ranged from 0.77 to 0.89. A significant IAA increase was recorded in plants after ammonium nitrogen sidedress application and it was confirmed that applications of nitrate reduced IAA concentration (correlation between NO3- and IAA for AS treatment R2 = 0.77-0.94).

Thirty-day-old barley (Hordeum vulgare L. cv. Zafer-160) calli were treated with 0, 0.5, 1 µmol/L 22(S),23(S)-Homobrassinolide (HBL) for four weeks. Transfer of calli to hormone-free or HBL-supplemented medium caused an increase in total soluble protein and DNA contents and a decrease in superoxide dismutase (SOD; E.C. 1.15.1.1) activity. 0.5 µmol/L HBL caused the highest protein and DNA levels and the minimum decrease in fresh weight; while the lowest superoxide dismutase activity was recorded in 1 µmol/L HBL-treated group. Calli developed nodular structures and green cell clusters after transfer onto hormone-free or HBL-supplemented medium. Methylation-sensitive restriction fingerprinting (MSRF) analysis detected a few changes in methylation between control and 0.5 µmol/L groups. Combination of data led us to the conclusion that HBL favoured regeneration and shoot development and may ameliorate tissue culture stress in a mechanism, which is independent of cytosine methylation.

The large-diameter time-domain reflectometry soil water sensors placed horizontally in a structured loamy soil are very sensitive to rapid preferential percolation events. Their readings on these occasions rise considerably, often becoming higher than the native soil's porosity. The effect is caused by gaps between the native soil and the sensors. The geometry of the gaps, even if filled with soil slurry at installation, is not exactly reproducible, which leads to sensor-to-sensor variability of readings. Field calibration in percolation-free periods lead to non-unique trajectories rather than monotonous calibration curves, which can be commented in terms of soil heterogeneity and the dual porosity theory. Data of two typical percolation events are presented. Sensors of this type can be used for detection of preferential flux.

Field experiments with reed canary grass were conducted during 1996-2000 at four different sites (Ruzyně, Troubsko, Lukavec and Chomutov in the Czech Republic). In the period 2001-2009 the experiments were run at Ruzyně and Lukavec. During vegetation the following indicators were monitored: the course of weather in individual years at given sites and infestation of stands by pests and diseases. Furthermore, we monitored the effects of N application rates, year, site, as well as the effect of harvest time on yields of harvested biomass, moisture content, elements content, and energy content. The content of heavy metals in soils and subsequently in plants was also monitored. The zoning methodology was created for reed canary grass. The highest average dry matter yield of reed canary grass harvested in late autumn was achieved for comparable periods during 1996-2000 at Ruzyně (8.33 t/ha), and the lowest at Chomutov (6.80 t/ha). The greatest effect of N fertilization on yields was recorded at Ruzyně, where the N2 rate (80 kg/ha) increased the yield on average by 28.6% in comparison with the non-fertilized variants. Average loss of biomass over winter was 23.0%.

Field experiments were conducted at the Agricultural Engineering Experimental Farm of The Federal University of Technology, Akure, during 2006/2007 and 2007/2008 seasons to investigate the response of cassava under drip irrigation. The experiment was laid out in a randomised complete block design (RCBD) with three replications. The treatments were based on four different water regimes; with T100 receiving 100% available water (AW), T₅₀ and T₂₅ receiving 50% and 25% of AW and T₀ with zero irrigation (control treatment). Disease free stems of the cassava cultivar TMS 91934 were planted at a spacing of 1 m by 1 m. The results indicated that T₁₀₀ full treatment produced the highest average total dry matter yield of 49.12 and 37.62 t/ha in 2006/07 and 2007/08 cropping seasons, respectively. However, the average total dry matter production in T₅₀, T₂₅, and T₀ showed significant differences in their values. Low total dry matter yields of 7.12 and 5.92 t/ha, respectively, were associated with T₀ for the two cropping seasons. The total water use of 1491.75 and 1701.13 mm was recorded for T₁₀₀, while total water use of 729.00 and 651.13 mm were obtained for T₀ in the two cropping seasons. The water use efficiency determined for the two cropping seasons ranged between 7.38 kg/ha and 32.93 kg/ha. The percentages of total water applied from total water use for T₁₀₀ were 51.11% and 61.72%, while 14.83% and 17.85% were recorded for T₂₅ for 2006/07 and 2007/08 cropping seasons, respectively.

Heavy metal (Cu, Zn, Cd, Pb and Fe) concentrations in salt marsh sediments of the Karnafully River coast were investigated in this study. Sediment samples of four different sites were analysed for selected heavy metals by AAS (atomic absorption spectrophotometry). The mean values of heavy metals were 45.79 µg/g for Cu, 105.0 µg/g for Zn, 0.43 µg/g for Cd, 26.70 µg/g for Pb and 3297.38 µg/g for Fe in this study. It is observed that Pb and Cu have positive correlations (r = 0.370, P < 0.05) and Fe and Zn have negative correlations (r = -0.729, P < 0.05) in the correlation matrix. Except Fe, all the metals were found at contamination levels which may indicate a recent and continuous contamination from domestic and industrial discharges. The results of sediment samples showed that the Karnafully River coast is facing heavy metal pollution.

Oil with higher a content of ω-3 polyunsaturated fatty acids (PUFA) was administered for 4 weeks to experimental germ-free piglets and from 21 days of age the piglets were inoculated perorally with probiotic micro-organisms Lactobacillus casei subsp. casei. Control piglets were administered saline solution in identical doses and starting from the fourth week were also supplemented with probiotics. At the age of 28 days, the number of Lactobacillus casei subsp. casei that had adhered to jejunal mucosa of experimental piglets was significantly higher (P < 0.05) compared to that in the control group. The differences in the number of lactobacilli, which colonised mucosa of the ileum and colon of experimental and control piglets, were insignificant. With the exception of the stomach, the pH level in the digestive tract of piglets was lower in the experimental piglets. Significantly higher levels of propionic (P < 0.05), acetoacetic and succinic (P < 0.01) acids were observed in germ-free experimental piglets in colon. The differences in the level of lactic and acetic acid were insignificant. PUFA-potentiated probiotics positively affected the adhesion of lactobacilli, pH and the level of organic acids in the digestive tract of germ-free piglets. Supplementation of oil containing ω-3 PUFA significantly increased the blood level of α-linolenic, eicosapentaenoic and docosahexaenoic acids in experimental piglets at the expense of arachidonic acid in comparison with the control.

A six year old crossbred male dog presented with non-pigmented, pale pink, unhaired, ulcerative tumor of the medial canthus and upper eyelid region. As treatment, surgery was proposed. During the surgery complete excision of the tumor was performed. The extent of skin loss, especially in the upper eyelid required plastic reconstruction by pedicle flap transposition. The soft skin of the cheek was chosen as a graft donor site. The postoperative period proceeded without any complications and ended with successful engraftment. The soft cheek skin did not impede movement of the upper eyelid. Histopathological examination indicated histiocytoma and tumor-free excisional margins were confirmed. There has been no recurrence during the subsequent 12 month period.

This paper reports the isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens). The microorganism was isolated from cutaneous lesions, identified by the commercial API 20 C AUX system, and confirmed by sequencing. Topical treatment with sertaconazol resulted in complete clinical recovery of the animal and repeat testing did not result in the recovery of the yeast from the healed lesion sites.

Bird skin has a number of specific properties. The uropygial gland is a significant skin gland in many species. The secretion of this gland is particularly necessary for maintaining physical characteristics, including feather waterproofing. In some bird species this gland secretion has a repellent effect against potential mammalian predators; in other species it affects the final colour of feathers. In the investigated species of storks (genus Ciconia), secretions of the uropygial gland have been found to be mixtures of monoester waxes, diester waxes, triester waxes and triglycerides. Wax diesters were also found in the red knot Calidris canutus (order Charadriiformes). Lipid substances in the secretions of the rock dove (Columba livia) consist mainly of unsaturated fatty acids (59% secretion; mostly oleic acid - 37%, linoleic acid - 6% and arachidonic acid - 7%). Free fatty acids, which are decomposition products of epidermal lipids, can regulate microbial colonization of skin (e.g., by modification of pH); a shift of these values was detected in poultry in battery husbandry. Analysis of fatty acids from lipids shows the influence of age, diet, and also the relationship to feather pecking - the individual composition affects the smell and taste, and thus the attractiveness to other individuals. The antibacterial activity of skin secretions has been demonstrated. Secretions of the hoopoe (Upupa epops) have besides the function of maintenance of physical properties of feathers also a repellent effect on parasites and predators. Its active substance is a peptide bacteriocin, produced by strains of Enterococcus faecalis. This substance is active against a number of both G + and G-bacteria and helps to sustain the nest hygiene, it is also effective against Bacillus licheniformis that produces keratin-decompositing enzymes. A similar antimicrobial activity of uropygial secretion against bacteria which degrade feathers was demonstrated in the wild house finch Carpodacus mexicanus. Changes in skin microflora have been demonstrated in parrots kept for breeding in comparison with those living in the wild, which may have significance for husbandry practices and veterinary care. Passerines of the genus Pitohui and Ifrita living in New Guinea store in their skin and feather batrachotoxins, which they receive from food - beetles of the genus Choresine. These toxins are active against parasites (e.g. lice - Phthiraptera). In contrast, substances that act as potential attractants for hematophagous insects (e.g. mosquitoes of genus Culex) were found in the skin of chickens. Alcohols, ketones and diones were detected in these substances. The composition of uropygial gland secretions may be a guide in assessing the relatedness of bird species. Feather waxes can be analyzed also from old museum specimens. Lipid-enriched organelles, multigranular bodies in the epidermis mean that zebra finches (Taeniopygia castanotis) are facultatively waterproof, which appears to have a function in protecting the organism against dehydrating when water is unavailable.

Claw horn disorders, infectious diseases of hooves and leg injuries cause lameness in dairy cows. However, such diseases as sole haemorrhages, sole ulcers or white line diseases, cause clinical lameness. Lameness reduces milk production, the fertility of cows and also causes earlier culling of cows, as well resultings in a deterioration of their welfare. In this review we focus on the impact of lameness on bovine behaviour. The time spent lying down is an important behaviour of dairy cows. As an increased locomotion score is associated with an increased percentage of cows lying down, also the position of cows within the milking parlour is associated with lameness. Lame cows are more likely to present toward the end of milking. Clinical lameness is a chronic stressor, reducing progesterone concentrations prior to oestrus, and resulting in reduced sexual behaviour; however, lame cows have the same potential period of oestrus when compared with non-lame cows. Hoof diseases, particularly those which are a source of pain, also reduce animal welfare. A high standard of cow welfare may be achieved by improving the lives of animals and the people who work with them. A lack of comfort while lying presents a significant risk for lameness. Improvements in comfort on more than 75% of farms (32 out of a total number of 53 farms) reduced the incidence of mastitis, while on 42 farms it reduced the prevalence of lameness. The keeping of cows on the straw bed of stalls does not only improve animal welfare, mainly through the greater comfort of the floor, but has also been showed to increase eating and ruminating behaviour. Cows also prefer straw to sand bedding and lay down longer on straw than on sand; however, cleanliness and hoof health have been shown to be better on sand. Apart from comfort, the main factors which promote improvements in bovine welfare and health, include good management of dairy farms, keeping cows in free stalls with accompanying regular exercise, and a long time spent at pasture. The prevalence of clinical lameness was demonstrated to be higher on farms using mattresses when compared with farms using deep-bedded stalls. No differences were found in behaviour among cows with different degrees of lameness housed in mattress stalls. Hence, measures of laying behaviour are not good indicators of lameness.

Bovine tuberculosis (TB) is an important neglected zoonosis in Cameroon, where many communities depend on their livestock for livelihood and the incidence of human TB and TB-HIV/AIDS co-infection are high and increasing annually. The aim of this study was to estimate the prevalence of bovine TB in cattle in the highlands of Cameroon. The magnitude and trend of detecting TB lesions in slaughtered cattle (1994 to 2010) and tuberculin skin tests (TST) in 2853 cattle (84 herds) of 39 livestock rearing communities were analysed. Of 129 165 slaughtered cattle inspected, 599 (0.46%; 95% CI: 0.43%-0.50%) showed suspected TB lesions among a total of 983 (0.76%; 95% CI: 0.71%-0.81%) identified pathologies. The monthly TB detection rates ranged from 0.30% (95% CI: 0.20%-0.40%) to 0.81% (95% CI: 0.64%-0.98%) and annual rates from 0.04% (95% CI: 0%-0.11%) to 1.46% (95% CI: 1.22%-1.69%). The rates were not affected (P < 0.05) by season and fluctuating peaks were also recorded. The comparative TST revealed that bovine TB was widely distributed in live cattle (4.67%; 95% CI: 3.89%-5.44%) and was higher (χ² = 17.50, P ≤ 0.001) in the Western highlands than Adamawa plateaux. Comparative TST bovine TB reactors were higher (P < 0.05) in cattle managed in semi-intensive and beef production systems compared to the others. Animals in small herds showed higher (χ² = 4.283, P = 0.038) rates than those in large herds. Bovine TB prevalence in exotic/upgraded cattle was comparable to that of the Red Bororo zebu but higher than the rates in Guadali (χ² = 4.971, P = 0.026) and White Fulani (χ² = 5.6, P = 0.018) zebus. Among the indigenous zebus, the rate was higher in Red Bororo than the Guadali (χ² = 6.244, P = 0.012) and White Fulani (χ² = 6.568, P = 0.010). Sex did not influence (χ² = 0.410, P = 0.522) bovine TB prevalence in this study but diagnosis of the disease was higher (χ² = 5.787; P = 0.016) among adult/older cattle than in younger animals. Further analysis of the TST responses revealed that atypical mycobacterial infections was widespread and 6.83% of tested animals showed positive reactions at both bovine and avian tuberculin injection sites and a strong association (χ² = 2.512; P = 0.113) between skin responses to both tuberculins. The study confirms that bovine TB is prevalent in live cattle and meat production abattoirs in Cameroon and we recommend strict a interpretation of TST results for maximum diagnosis of the disease in the local environment. A need for comprehensive investigation of the molecular epidemiology, zoonotic risks and the public health importance of bovine TB in Cameroon cannot be overemphasised.

The article focuses on selected aspects of risk management in agricultural business with the aim to discuss and compare different hedging methods which are relevant for managing the commodity risks associated with agricultural production. The article provides a broader context for understanding the risks and possible responses to it and analyses four basic hedging strategies - commodity futures, forward contracts, options and option strategies. The substance, advantages and disadvantages of each hedging technique are pointed out and compared to each other with the conclusion that there is always some kind of trade-off between the advantages and disadvantages of the particular strategies. The farmers shall, therefore, consider both all aspects of the relevant strategies and their expectations, before they make the final decision which instruments to use.

The food market is created by the farmers, whose importance arises from their production of the raw materials from which food is made. These products are processed and supplied to the retail chains, where they are offered to the end consumers. The food market is further regulated, and in a certain sense limited, by the EU organs, as well as by the domestic agricultural policies, by the means of a whole host of directives, norms and regulations. The aim of this article is to define the individual organs on the food market in the CR, to evaluate their work in the food chain from the production of the primary raw materials to their distribution to the end consumer. The article will also present the selected regulatory factors of the market in the context of the EU agricultural policy.

The comprehensive risk analysis of a business such as farming entails questions on what is at stake, how important is the risk concern and how to deal with it. We performed a sequential mixed method, with the in-depth interviews in the first stage (n = 35), followed by a survey on the Flemish FADN (n = 614) in the second, to investigate the farmers' risk perception, the attitudes towards risk and the perceived usefulness of the risk management strategies. We find that, rather than the short-term volatility in prices, the longer term co-evolution of expenses versus receipts is of a major concern to farmers, next to the land availability and the policy risks. Farmers are shown to be only slightly risk averse, rather risk neutral even. Further, our results suggest that farmers do not consider extensively studied risk management strategies such as contracts, futures and insurances, a valid option for their farm, and put more faith in internal strategies such as the debt management, the liquidity management and diversification. Last, risk management is to a substantial degree performed at the household level, rather than at the farm level, with strategies such as cutting the private expenses and the off-farm employment. These results hardly differ according to the farm and farmer characteristics.

In the study, some important herbal agricultural products with respect to their production have been clustered, in addition to determining the most important or the best countries in terms of the production of certain herbal agricultural products by using the factor analysis. The FAO data set has been used in obtaining production of 30 agricultural products in 86 countries. 8 factors have been achieved by considering the Eigen values the numbers of which are greater than one. Each factor contains certain herbal agricultural products. First factor explains 40.51% of the total variation whilst the last factor explains only 3.89% of the total variability. 10 best countries for each factor have been revealed.

The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (AluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.

The feasibility of metabolomic fingerprinting approach based on ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-QTOFMS) was studied to assess its ability to discriminate between maize varieties, and to show the associations between them on the metabolomic level. The non-targeted metabolomic analysis was applied to assess the variability within two varieties grown under different environmental conditions and to characterise the association within a sample set comprising both conventional and transgenic (MON-ØØ81Ø-6) maize varieties cultivated under the same environmental conditions (locality). Typical metabolomic fingerprints were established for individual plants. The plants representing two varieties formed well separated clusters. Metabolomic fingerprints of the second sample set enabled their unambiguous discrimination. The differences in metabolomic fingerprints between maize varieties were identified and documented by grouping in PCA and/or CA. The results indicate a similar genetic basis of transgenic maize varieties as they descend from a MON 810 event. The results explicitly showed that the variability of the metabolites in MON 810 did not exceed the ranges measured within the conventional varieties, thus supporting the concept of substantial equivalence.