Fulltext search in archive

: The paper describes the basic potential methods and their costs of maintenance of forest lands in protective zones under high-voltage power lines (HVPL) 400 kV and 220 kV. In the present situation and with available information and data, it was possible to accomplish the average cost calculations for the whole area of the Czech Republic. The paper analyses the following mechanical and chemical methods of maintenance of forest lands: full-area scrub clearing with power saw (PS), selective individual scattered clearing of trees with PS, full-area scrub clearing by milling, full-area scrub clearing by the application of herbicides and arboricides, selective individual scrub clearing of individual trees by cutting followed by spray treatment with arboricides. The maintenance of forest lands in zones of HVPL (scrub clearing) by milling belongs to the economically most convenient mechanical methods, for example when removing 3,000 trees.ha^-1, the cost of this option is 20,000-23,000 CZK.ha^-1ha in a period of 3-year periodic frequency, while the most expensive method is selective individual scrub clearing of scattered individual plants with PS at costs of around 84,000 CZK.ha^-1.

This paper documents the development of soil conditions in the set of 32 permanent research plots in the Krkonoše (Giant) Mts. These plots represent an altitudinal gradient covering the ecosystems of beech, mixed beech-spruce and spruce stands. In all plots, representing the site conditions of the highest areas of the mountain range, standard soil pits were prepared and the soil sampling was performed in autumn of years 1980, 1993, 1998, 2003 and 2009. The results reflect extreme site conditions, soil acidification, large scale surface liming and in minor extent also different tree species composition of the stands. The general type of the soil-genesis is represented by the podzolisation, overlapping the other soil-genetic factors, including the tree species composition. Nevertheless, this development is mostly expressed in the spruce stands. The beech dominance and/or co-dominance are reflected especially by more efficient N-cycling, higher pH, S and V values and fluctuation and lower extractable Al3+ content. More efficient cycling in beech ecosystems is insignificantly documented for plant available phosphorus, calcium and magnesium contents; on the contrary higher dynamics for iron ions was registered in the spruce stands. The long-term soil dynamics with a hysteresis (evident on the base of ordination analysis) can be divided into some periods - processes of acidification (typical in the 1980's samples), liming (main effect in 1993 and 1998) and regeneration (2003, 2009). Other features, important for the soil development, are probably related to the vegetation change, but this relation is not statistically significant.

This study is focused on substitute European larch stands in the Krušné hory Mts. (northern part of the Czech Republic). Research was conducted within larch thinning experiment Kalek (780 m a.s.l. in the category Piceeto-Fagetum oligo-mesotrophicum - Calamagrostis villosa). Results showed that the aboveground biomass of the investigated substitute unthinned larch stand represented approximately 102 thousand kg of dry matter per ha at the age of 20 years. Stemwood (ca 59%) is the most important part of the aboveground biomass. Needles, live and dead branches accounted approximately for 6%, 17% and 11%, respectively, and stem bark only for 7%. At the age of20 years, the investigated substitute unthinned larch stand accumulated: nitrogen - 307 kg, phosphorus - 21 kg, potassium - 136 kg, calcium - 122 kg, magnesium - 53 kg per hectare. Thinning with consequent removal of aboveground biomass may result in nutrient losses. Especially, the removal of whole tree biomass by thinning for chipping in areas previously degraded by acid deposition may result in calcium and magnesium deficiency because of their low content in forest soil. On the other hand, thinning supported faster growth of trees left after thinning and consequently faster biomass and nutrient accumulation. Our results supported the recommendation that the use of biomass from thinning for chipping should be limited to stemwood only and the remaining aboveground biomass (mainly needles and branches) should be left in the forest ecosystem for decomposition in conditions of the historically disturbed area of the Krušné hory Mts.

Climate change is expected to influence the distribution and population dynamics of many insect pests, with potential severe impacts on forests. Spruce bark beetle Ips typographus (L.) (Col.: Curculionidae, Scolytinae) is the most important forest insect pest in Europe whose development is strictly regulated by air temperature. Therefore, climate change is anticipated to induce changes in the pest's distribution and development. We used the PHENIPS model to evaluate climate change impacts on the distribution and voltinism of spruce bark beetle in the Czech Republic. Two future time periods - 2025-2050 (near future) and 2075-2100 (distant future) - are addressed. The period 1961-1990 is used as the reference. We found that while a two-generation regime dominated in the Czech Republic in the reference period, significant three-generation regime regions are projected to appear in the near future. In the distant future, the three-generation regime can be expected to occur over all existing coniferous stands in the Czech Republic. The analysis of altitudinal shift of n-generation regime regions indicates noticeable expansion of Ips typographus development to higher elevations, leading for example to disappearance of one-generation regime regions in the distant future. Uncertainties and limitations of the presented findings are discussed as well.

The survey was conducted in three selected areas of the Czech Republic in 2008, with the results processed in 2009. Forests visitors received inquiry forms (face to face interviews, random sample). The total number of visitors on 8 survey days was 7,369. The total number of filled-in questionnaires in the three areas was 1,122. Tree planting and tree protection are considered as the most important forest operations, followed by road and stream bank maintenance. On the contrary, timber transport and harvesting are considered as the least important activities. The nature-protecting function is considered as the most important forest function, followed by soil-conservation, climatic, hydrological and health (recreational) functions. Timber production and non-timber production are the least important functions according to the respondents. 20% of respondents claimed that increased costs needed to improve non-market forest functions, used by the visitors, should be partially or fully financed from the timber sales revenues, while only 6.5% of respondents say the costs should be partially or fully financed from payments by the users of forest functions.

The life cycle operation phase of John Deere 1490D energy wood harvester from the aspect of energy audit and environmentally negative influence caused by emissions were evaluated. Energy audit quantifies energy used in the form of fuels and oils as well as energy expended for manufacture, transport and distribution of these fuels and oils. Emissions produced by operation are determined according to the consumption of fuels and oils based on emission factors. We also made a comparison of the general calculation of costs based on data provided by the manufacturer with costs ascertained in real operating conditions. The costs are divided into purchase, operating and other costs. Total costs are subsequently converted into unit costs according to the assumed productivity of the machine, its estimated lifetime, and the mean real throughput of the machine. The machine throughput, and thus also its operating economics, is greatly dependent on the character of the processed material, its stem volume, moisture, way of dendromass preparation, and operator's skills. Energy use was calculated at 74.4 MJ.FU^-1 (functional unit) related to fuel consumption and 13.4 MJ.FU^-1 related to the consumption of oils. The total energy use of the life cycle operation phase of the slash bundler was determined as 87.8 MJ per each bundle produced. The real productivity of the machine in the given operating conditions was several times lower than the productivity indicated by the manufacturer.

Information on the historical background of the present forest conditions is needed for proper decisions on forest management. Disturbances play a major role in the formation of forest structure and composition. This paper compares the present break-up of mountain spruce stands in the Bohemian Forest (in the Šumava Protected Landscape Area) with conditions during their initiation using dendrochronological techniques. On three selected localities we established two study plots within relatively old stands of unknown origin. Stands were recently broken up by a windstorm. The present situation was described by diameter and decay class distribution. To describe the situation at the stand initiation, we cored at least 40 stems on each plot to get the age structure and growth series of trees. Disturbances were marked by discovering synchronous releases on tree-ring series. Main population waves were initiated in association with severe disturbances during a relatively short period, leading to the establishment of relatively even-aged, homogeneously looking stands. The disturbances were synchronized between plots and accounted for by historically known windstorms or bark beetle outbreaks; notwithstanding, logging as a disturbance factor is also particularly possible.

The Fusarium species associated with maize are widely distributed in Malaysia. Eight Fusarium species were obtained in this country. A series of field samplings was conducted from 2006 to 2008, when 167 Fusarium isolates were obtained from maize plants in seven locations throughout Malaysia. The determination was based on micro- and macromorphological features (growth rates, colony features, mode of production of microconidia, macroconidia, conidiophores, and chlamydospores). F. proliferatum (29.9% isolates), F. semitectum (22.2% isolates), F. verticillioides (13.7% isolates), and F. subglutinans (12.6% isolates) were found out most frequently. F. equiseti, F. pseudograminearum, F. oxysporum, and F. solani were also isolated. This is the first report on the occurrence of F. equiseti, F. pseudograminearum, and F. subglutinans associated with maize plants in Malaysia.

Occurrences of pea aphids and their natural enemies (syrphids, mummies caused by entomopathogenic fungi Beauveria sp. and by the parasitic wasp Aphidius ervi) were compared in monocultures and mixtures of field peas and spring cereals in three seasons (2008-2010). At the beginning of colonisation, the occurrence of aphids was not substantially influenced by intercropping with cereals. However, the numbers of pea aphids located on inflorescences started to decline earlier in mixtures compared with monoculture. More syrphids (eggs + larvae) were found in mixtures than in monoculture, and more syrphid eggs were found in young aphid colonies (10 to 20 individuals) in mixtures. Intercropping did not influence the occurrence of fungal mummies (Beauveria sp.), but mixtures tended to have more aphid colonies infested by A. ervi in 2008 and 2009.

The morphology and biology of immature stages and the distinguishing characters of generations in summer are briefly reviewed. The time of migration of the overwintered generation and the use of the median flight time are given. The longevity of overwintered adults, and the start and course of oviposition by overwintered females were observed mainly during June and July. The interval between adult emergence and flights from carrots appears to be very short, 2-3 days. Departure from developmental summer host plants culminates at the beginning of September. The sex ratio of the overwintered generation is not persistent, since it is usually female-biased. In emerged adults in summer the ratio is 50:50, with weak protandry. The systemic action of sucking for the curling of leaves was found by Láska already in 1964. Inliterature records, both subspecies of Daucus carota dominate as developmental hosts, more recently Coriandrum sativum was shown to be more suitable than Petroselinum hortense. Norway spruce, and to a lesser extent other conifers, are predominantly mentioned as overwintering shelter plants. It is not clear how overwintering occurs in regions without coniferous forests, and only undefined shrubs or trees are given in the literature. The first damage was reported inDenmark about in 1896, spreading from about 1918 over just a few years to the other Fennoscandian countries, and later in some other European countries. The spring migration is discussed, which occurs in central and northern Europe on the same date, perhaps explicable by the orientation of this pest or its particular populations to long days, despite the climate and phenology of each region. It is hypothesised that a particularly aggressive race evolved inSjaelland (Denmark) at the turn of the nineteenth and twentieth century, which later spread to other regions of northern and centralEurope.

Peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae), has been a serious pest in the last decade attacking a wide range of fruits in Egypt. The toxicity of Malathion, Diazinon, Methoxyfenozide, and Lufenuron to adult males and females of Bactrocera zonata was studied under laboratory conditions. Diazinon was the most toxic among the tested compounds followed by Malathion, Lufenuron and Methoxyfenozide to Bactrocera zonata at 24 h post treatment, the respective LC₅₀ values were 0.20 ppm, 0.48ppm, 8.97ppm, and 9.73ppm for males and 0.26 ppm, 0.91ppm, 11.26ppm, and 14.12ppm for females. At 48 h post treatment Diazinon was the most toxic followed by Malathion, Methoxyfenozide and Lufenuron to Bactrocera zonata, LC₅₀ values were 0.09ppm, 0.34ppm, 1.60ppm, and 1.88 ppm for males and 0.14 ppm, 0.44ppm, 1.68ppm and 2.17 ppm for females. At 72 h post treatment Diazinon was the most toxic followed by Malathion, Lufenuron and Methoxyfenozide to Bactrocera zonata, LC₅₀ values were 0.02 ppm, 0.13ppm, 0.22ppm and 0.51ppm for males and 0.07 ppm, 0.16ppm, 0.55 ppm and 0.62 ppm for females. It is observed that LC₅₀ values for treated adult females increased more than in the treated adult males at 24 h, 48 h, and 72 h post treatment. It means that the adult males were more susceptible to the tested insecticides than the adult females.

The susceptibility to five new insecticide mixtures: chlorosan, feroban, cygron, engeo, and kingbo was studied in the 2^nd and 4^th instar larvae of the cotton leafworm Spodoptera littoralis (Boisd.). The efficiency and residual effects of these compounds against S. littoralis under field conditions were also investigated. Obtained results revealed that feroban was the most effective compared with the other toxicants, while engeo was the least toxic insecticide in both instars after 2 and 5 days from treatment. Data also indicated that feroban had the longest half-life (Lt₅₀) while engeo recorded the shortest one. Biochemical analysis showed that the tested compounds caused pronounced changes in acetyl cholinesterase and phenol oxidase.

Nitric oxide (NO) is a gaseous diatomic molecule with a wide variety of physiological and pathological implications in plants. Presence of unpaired electron in its molecular orbital makes it highly reactive; it can react directly with metal complexes, radicals, DNA, proteins, lipids and other biomolecules. Nitric oxide (NO) and reactive oxygen species (ROS) are known to play essential role in a number of important plant physiological processes. This manuscript reviews the role of NO on these processes during various biotic and abiotic stresses.

In this experiment we proved an effect of dolomite limestone on chemical immobilization in soil contaminated by trace metals, namely Cd, Pb, and Zn. Primary, we set up lysimeter pot experiment to measure soil leaching without vegetation. Willow clone (S. × smithiana Willd) was cultivated in the second lysimeter pot as a new approach to monitor Pb, Cd and Zn leaching, which was affected by soil liming (used in 1% rate). At the time of both harvests, aboveground biomass increased significantly at the amended variant. After the second harvest aboveground biomass production increased by 80% in comparison with the first one, Cd and Zn concentration in biomass decreased 2-fold and 3-fold, respectively. Dolomite limestone as a process of liming: (i) restricted metals leaching from the soil substrate; (ii) reduced metal uptake by willow; and (iii) increased biomass production of willow. Liming also alleviated the plant stress imposed by risk elements resulting in better plant growth and lower levels of stress markers (total nitrogen content and the main amino acid metabolism parameters in the willow leaves) yet through different mechanisms.

This study aims to reveal and to compare effects of two different systems of nitrogen (N) nutrition (sidedress application or injection application) on toxicity of NH₄⁺ and mixed nutrition. We investigated whether NH₄⁺ or mixed (NH₄NO₃) application causes significant changes in the endogenous levels of cytokinins (CK), whole plant N and their effects on yield of selected plants. Ammonium sulphate or ammonium nitrate were used as N source in the pot experiment. The yield of Festulolium and Trifolium pratense L. above-ground biomass and roots was more substantially enhanced after sidedress application of both ammonium sources in comparison with injection application. Our results confirmed that the accumulation of CKs in plants is in correlation with their N content (R² = 0.66-0.98). Proportions between individual CK forms remained relatively steady and their dynamics exhibited similar trends after N application. Our results indicate that the negative effect of the application of NH₄⁺ on the growth of Festulolium and clover plants could be effectively modulated by the presence of NO₃^-.

Aluminium (Al) mobilisation in the forest soils is a serious problem due to the soil acidification. The rate and magnitude of leaching of Al and other elements and compounds from soils can be examined by means of percolation experiments. Aluminium elutriation was studied under laboratory conditions using undisturbed samples of forest topsoil from the Paličník area in the Jizera Mountains (Czech Republic), which originated under two different vegetation covers: European beech (Fagus sylvatica L.) and Norway spruce (Picea abies (L.) Karst). Ponding infiltration was performed using three subsequently applied solutions. KCl solution was used to simulate the soil solution. Solutions with sulphates and nitrates addition (of two different pH values) were used to simulate acid rainfall. Passing liquid phase was analysed with respect to Al content and aluminium speciation. Differences were found in Al content and transport between different soils under spruce and beech covers. The soil sample under the spruce forest (SF sample) had a higher initial Al content than the soil sample under the beech forest (BF sample). As a result, the aluminium leaching from the spruce soil sample and the final content of water-extractable Al in the soil (Al content after the leaching experiment) were higher compared to the beech soil sample. This suggests that Al mobility and potential toxicity in the beech forest are grater than those in the spruce monoculture when studied in the acidification endangered areas.

In this study, the effects were investigated of salinity, foliar and soil applications of humic substances on the growth and mineral nutrients uptake of Corn (Hagein, Fardy10), and the comparison was carried out of the soil and foliar applications of humic acid treatments at different NaCl levels. Soil organic contents are one of the most important parts that they directly affect the soil fertility and textures with their complex and heterogenous structures although they occupy a minor percentage of the soil weight. Humic acids are an important soil component that can improve nutrient availability and impact on other important chemical, biological, and physical properties of soils. The effects of foliar and soil applications of humic substances on the plant growth and some nutrient elements uptake of Corn (Hagein, Fardy10) grown at various salt concentrations were examined. Sodium chloride was added to the soil to obtain 20 and 60mM saline conditions. Solid humus was applied to the soil one month before planting and liquid humic acids were sprayed on the leaves twice on 20^th and 40^th day after seedling emergence. The application doses of solid humus were 0, 2 and 4 g/kg and those of liquid humic acids were 0, 0.1 and 0.2%. Salinity negatively affected the growth of corn; it also decreased the dry weight and the uptake of nutrient elements except for Na and Mn. Soil application of humus increased the N uptake of corn while foliar application of humic acids increased the uptake of P, K, Mg,Na,Cu and Zn. Although the effect of interaction between salt and soil humus application was found statistically significant, the interaction effect between salt and foliar humic acids treatment was not found significant. Under salt stress, the first doses of both soil and foliar application of humic substances increased the uptake of nutrients.

We have estimated soil organic carbon and crop yield changes under distinct climate change scenarios for the Kočín farm in Slovakia. Two regional climate change scenarios, i.e. the A2 and B2 SRES emission scenarios, and a reference climate scenario have been included into the bio-physical process model EPIC to simulate the effects on the topsoil organic carbon stocks and crop yields for the period of 2010-2050. In addition, we have used the data from several fields of the Kočín farm including the soil data, crop rotational and management data as well as topographical data. The topsoil organic carbon stocks show a decreasing trend for the period of 2010-2050. Among all crop rotation systems and soil profiles, the losses over the period are 9.0%, 9.5%, and 10.7% for the reference, A2, and B2 climate scenarios, respectively. Increasing temperatures accelerate the decomposition of the soil organic carbon particularly when soils are intensively managed. The soil organic carbon changes are crop-rotation specific, which is partly due to the climate scenarios that affect the crop biomass production differently. This is shown by comparison of the crop yields. We conclude that EPIC is capable to reliably simulate effects of climate change on soil organic carbon and crop yields.

One peculiar feature of the inland valleys abundant in West Africa is their site-specific hydrology, underlain mainly by the prevailing landforms and topography. Development and management of these land resources under the increasingly popular sawah (a system of bunded, puddled and levelled rice field with facilities for irrigation and drainage) technology is a promising opportunity for enhancing rice (Oryza sativa L.) production in the region. Information on the variations in selected soil physical properties as influenced by the prevailing landforms may serve as a useful guide in site selection. This is of practical importance since majority of the inland valleys are potentially unsuitable for sawah development and most farmers in the region are of low technical level. Three landforms (river levee, elevated area and depressed area) were identified within a sawah field located in an inland valley at Ahafo Ano South District of Ghana. Each of these landforms was topsoil-sampled along on identified gradient (top, mid and bottom slope positions). Parameters determined included particle size distribution, bulk density, total porosity and field moisture content. The soil is predominantly clayey. There were no variations in the particle size distribution among the slope positions in the river levee. Overall, the river levee had lower silt content than the elevated and the depressed landforms. The bulk density, total porosity, and gravimetric moisture content indicated relative improvements only in the depressed area in the order, bottom > mid > top slope. Irrespective of slope position, the three landforms differed in these parameters in the order, depressed > river levee > elevated. The sand fraction impacted negatively on the silt fraction and bulk density of the soil, both of which controlled the soil moisture status. Despite the fairly low silt content of the soil, the silt fraction strongly influenced the gravimetric moisture content (R² = 0.80). So too did the soil bulk density on the gravimetric moisture content (R² = 0.90). It is concluded that: (1) since the landforms more prominently influenced the measured parameters than the slope positions, the former should take pre-eminence over the latter in soil suitability judgment; (2) with respect to moisture retention, variations in silt fraction and bulk density of this and other clayey inland-valley soils should be used as guide in site selection for sawah development.

The present study describes pathogenesis and morphology of tuberculous and tuberculoid lesions in small terrestrial mammals, above all, in rodents. The most serious infectious agents that cause tuberculous and tuberculoid lesions in these animals are also cited. Besides others, the diseases caused by pathogenic mycobacteria that are members of Mycobacterium tuberculosis and M. avium complexes, M. lepraemurium, tularaemia, brucellosis and salmonellosis are included in the present study.

In the Czech Republic, most mycobacterial infections in pigs are caused by the Mycobacterium avium complex (MAC) and potentially pathogenic mycobacteria (PPM) derived from the environment. This study was undertaken after the isolation of PPM from various components of the environment of pig herds between 1996 and 2002 (Matlova et al., Veterinarni Medicina, 48, 2003, 343-357). Between 2003 and 2007, a total of 1114 environmentally-derived samples from 24 farms were examined. After staining according to Ziehl-Neelsen, acid-fast rods were found in 42 (3.8%) samples by direct microscopy, and PPM were isolated from 223 (20.0%) samples by culture. PPM occurred primarily in soil from the paddocks (53.8%), peat (53.2%), bedding (28.4%) and biofilm from the pipeline (21.0%). From MAC, M. avium subsp. hominissuis (MAH) genotype IS901- and IS1245+ was most frequent; M. avium subsp. avium (MAA) genotype IS901+ and IS1245+ and M. intracellulare genotype IS901- and IS1245- were isolated from one (0.4%) and three (1.3%) samples, respectively. The remaining isolates were identified as 19 other mycobacterial species: M. gordonae (n = 8), M. triviale (n = 6), M. flavescens (n = 3), M. nonchromogenicum (n = 3), M. terrae (n = 3), M. xenopi (n = 3), M. fortuitum (n = 2), M. chelonae (n = 2), M. chitae (n = 2), M. abscessus (n = 1), M. gastri (n = 1), M. kumamotonense (n = 1), M. marinum (n = 1), M. parafortuitum (n = 1), M. peregrinum (n = 1), M. porcinum (n = 1), M. scrofulaceum (n = 1), M. smegmatis (n = 1) and M. simiae (n = 1). The remaining 41 isolates of unidentified mycobacterial species did not contain the sequences IS901 and/or IS1245, specific for medically important members of MAC (MAA and MAH); a further 44 isolates were not tested due to their contamination or loss of ability to grow in vitro. A farm where MAH was often detected in the lymph nodes of pigs and in the environment between 1996 and 2002 (Period I), was selected for further investigation between 2003 and 2007 (Period II). A comparison of the findings of mycobacteria on the investigated farm in Period I and in the following Period II showed a significant increase (P < 0.01) in the occurrence of mycobacteria other than MAH, especially in peat samples.

Carprofen or meloxicam are nonsteroidal anti-inflammatory drugs (NSAIDs), which may elicit a variety of renal disturbances. Prior to this study, the effects of carprofen or meloxicam on renal function in pigs were unknown. A total of 21 clinically healthy Goettingen miniature pigs (36.9 ± 7.22 kg) were divided into three groups based on what they were administered - carprofen, meloxicam or saline. First, blood was collected from the jugular vein and urine by ultrasound-guided cystocentesis. Serum urea (U) and creatinine (CR), fractional clearance of sodium (FCNa), urine gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) activities, urine protein/creatinine ratio (UP/UC), urine gamma-glutamyltransferase/creatinine ratio (GGT/CR) and urine alkaline phosphatase/creatinine ratio (ALP/CR) and urine analysis - urine specific gravity (USG) and sediment microscopy were assessed before and seven days after daily intramuscular administration (IM) of saline (1.5 ml per animal), carprofen (2 mg/kg) or meloxicam (0.1 mg/kg). All animals had identical housing, feeding and unlimited water intake and had not undergone surgery or been administered any medication for three months prior to this. All pigs served as control groups for an experimental study of fracture healing using transplantation of mesenchymal stem cells and scaffolds. The data were analyzed using a one way ANOVA and a Mann-Whitney test (P < 0.05). In pigs receiving carprofen, serum urea and creatinine were significantly decreased, compared to the control (P < 0.01) or meloxicam (P < 0.05) groups. In animals receiving meloxicam FCNa was significantly increased (P < 0.05) and urine specific gravity significantly decrease (P < 0.05) compared to the pretreatment values. Two carprofen-treated pigs had a slight increase in renal tubular epithelial cells upon urine sediment examination. Intramuscular administration of carprofen or meloxicam in healthy miniature pigs for seven days causes no clinically important changes in selected renal parameters (without azotemia). However these changes indicate mild damage of renal tubules. Despite these findings, meloxicam or carprofen are recommended for analgesia in healthy pigs.

The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. This study followed the previous monitoring of the herd using a cultivation method which showed only a minimal infection load among the animals. Although no mouflon showed clinical symptoms, 35.7% and 80% of ewes were IS900-positive in 2008 and 2009, respectively. In seven out of 21 adult ewes, the presence of the IS900 sequence was determined in 2008 as well as in 2009. Between the first and second sampling, twenty-three mouflon lambs born and kept with the ewes were examined. Almost one third of them (30.4%) were proven to have the MAP sequence in their faeces. Also, 75% environmental samples from the mouflon farm showed positivity. Infected animals without clinical symptoms which low sensitivity cultivation does not detect represent a source of infection for other animals. Therefore, real-time PCR has a crucial role in paratuberculosis control programs, especially in control of the disease by the culling of infected animals.

A four-year old, 26.5 kg, Boxer bitch was presented to the Department of Reproduction in the Clinic of Dogs and Cats with a six month history of vulvar swelling and vaginal discharge. General gynaecological examination showed an extremely swollen, oedematous and tough-elastic highly irritable vulva. A vaginal smear revealed the presence of superficial cells and red blood cells. After repeated unsuccesful administrations of HCG and GnRH the owner of the bitch agreed to surgical treatment. An ovariohysterectomy was performed and on the left ovary a grapefruit-size structure was found. Three nodular structures were found retroperitoneally and were also dissected. Histological examination showed a disgerminoma in metastasises, mitotically active with a bad prognosis. However, at a check up twenty months subsequently the patient was still alive.

Studies of the migratory dynamics of cyathostomin infective larvae (L₃) in different seasons and with two types of irrigation were carried out over 12 months (September 2006 to September 2007) in the subtropical climate of the Baixada Fluminense region of Rio de Janeiro state, Brazil. Four faecal masses weighing 500 g each from naturally infected horses were placed in a Bermuda grass (Cynodon dactylon) pasture in the beginning of each season. Samples of faeces and grass were collected every 15 days until the end of each season. The highest recovery in faeces occured in Autumn (491 910 L₃/kg dried herbage) and in pasture was achieved in Winter (9 963 L₃/kg dried herbage). The lowest number of infective larvae recovered from faeces (55 100 L₃/kg dried herbage) and pastures (2 188 L₃/kg dried herbage) were achieved in Spring. The nonparametric Kruskal-Wallis test showed a significant difference in infective larvae recovery between the seasons. The collection time of the samples did not affect the larva recovery. The results suggest that in the conditions of the region studied, animals maintained in pasture are at permanent risk of infection.

Data of toxicological analyses for baits carried out in the Laboratory of Toxicology of the Department of Veterinary Clinics (University of Pisa) over a 10-year period are summarized. The 508 lures have been grouped according to their preparation features. This classification has generated six classes: (1) baits prepared with discarded or out-of-date food; (2) laborious and original/particular baits; (3) baits containing more than one toxic substance; (4) baits containing non-toxic material; (5) baits prepared with non-food material and (6) in vivo baits. The most commonly detected toxic substances in baits were organophosphorus and carbamate pesticides (48%), anticoagulant rodenticides (18%), zinc phosphide (12%), strychnine (9%), metaldehyde (8%) and others (5%). This survey shows that in Italy, the deliberate misuse or abuse of toxic substances intended to kill domestic animals, is very common and still far from being eradicated. Elaboration of a complex strategy, involving authorities as well as veterinarians and citizens, is the first step to defeat this harmful practice.

Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More than 10 000 cells per gram were present in four samples (7.8%). Such concentrations mean that one package of milk contains 5 million MAP cells, which are ingested by a bottle-fed baby over the course of several days. Premature babies and bottle-fed newborns can be affected by pro-inflammatory triggers from a huge number of mycobacteria despite not suffering from infection with bacteria or viruses often linked with the etiology of Crohn's disease.