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The investigation of foliage biomass development including LAI and the growth of spruce and beech stands in the juvenile stage (age 1 to 21 years) represents a special complementary study to long-term research of water balance components on the experimental forest hydrology area Deštné Mountainside. The experiment is located in the Orlické hory Mts. at the altitude of 900 m on a clear-felled area. In 1982 the spruce plantation was established at 1.5 ´ 1.5 m spacing (4,600 plants/ha) and the beech plantation at 1.0 ´ 1.0 m (10,000 plants/ha). According to tending programs applicable to forest stands in the Czech Republic, two improvement fellings were carried out (1995 and 2001) in spruce and none in beech. In 2002, some 1,550 spruce trees/ha (mean height 10.1 m) and 7,440 beech trees/ha (mean height 4.8 m) were recorded. At the end of the first vegetation season in 1982, the dry matter (DM) of foliage in spruce and beech amounted to 35 and 70 kg/ha, respectively. Five years later (1987), these values increased to 770 and 360 kg/ha in spruce and beech, respectively. At a stand age of 21 years, foliage DM was determined to be 11,940 kg/ha for spruce and 3,050 kg/ha for beech. At the same time, the leaf-area index (LAI) was calculated to be 5.55 and 5.94 in spruce and beech, respectively. The method of foliage biomass quantification, based on the determination of foliage DM of mean sample trees and stand density, enabled to acquire complementary data usable in long-term research of the water regime of spruce and beech and simultaneously to provide information on the potential of biomass production and LAI value of both main commercial species in mountain forests of the Czech Republic.

In order to determine the environment carrying capacity in terms of biomass production utilizable by game 360 representative sample plots (1 × 1 m) were laid out in the growing season on the basis of typological classification in forest stand of an area of 1,796.49 ha in the studied region of the Soutok Game Preserve, Židlochovice Forest Enterprise, Lanžhot Forest District. On meadows, pastures and others areas producing grass and herbs of a total area of 532.87 ha, other 57 sample plots were laid out and sample of biomass utilizable by game. Quantification of the biomass was carried out on the basis of the area cover of grass and woody undergrowth. In total, forest and non-forest land provides 14,659,851 kg grass and herb utilizable biomass. In forest stands production was found of 6,826,662 kg grass and herb biomass (on average 380 g/m²) and on meadows and pastures 7,833,189 kg (on average 1,470 g/m²). Moreover, production of 1,401,262 kg (on average 78 g/m²). Laboratory analyses were carried out of naturally dried-up samples of biomass and these values available energy were ascertained: the energy of grass and herb biomass amounted to 5.7 MJ/kg, the utilizable energy of wooody origin amounted to 4.03 MJ/kg. In view of the standardized game population size up the available food supply sufficient, because the energy requirement was fully covered by their daily quantitative consumption of biomass.

The use of skidding machinery in logging operations causes destructive effects especially on soil but has many advantages such as extracting long and heavy logs, optimum use of useful logging time and absence of defect in wood production quality and thus an increase in the added value of wood. In this research compartment 40 of the second district of Nav-Asalem in northern Iran at an altitude of 1,050-1,450 m above sea level was chosen in order to assess the amount of displaced soil volume and depth of ruts due to the traffic of Timber Jack 450C rubber wheeled skidder along the skid trail. Therefore, the correlations between two independent variables, longitudinal slope and soil texture, with two dependent variables, displaced soil volume and average depth of rut, along the skid trail were examined after 20 passes of skidder machinery. The results of this research showed a significant difference between the longitudinal slope increase of skid trail and the amount of soil volume displaced (P < 0.001). However, there was no significant correlation between the mean rut depth and different classification of longitudinal slope along the skid trail. The highest measured rut depth was 22 cm and occurred in the slope class of more than 25%. There was no significant correlation between the amount of displaced soil volume and rut depth with changes in soil texture along the skid trail.

Psocid (Psocoptera) taxocoenoses were studied in forest ecosystems of the Western Carpathian Mts. in 1997-2001. As a study frame, vegetation tiers (VT = altitudinal vegetation zones) of geobiocoenological or forest-typological system were used. Lower units of forest typological system (forest type complexes) were used for the classification of ecological conditions and the material found in forest ecosystems of Abieti-fageta s. lat. communities (5^th fir-beech VT) was evaluated in detail. This VT is the most widespread in the regions under study (the Moravskoslezské Beskydy Mts., the Vsetínské vrchy Hills and Javorníky). 2,023 adults comprising 28 species were found in the 5^th VT. Caecilius burmeisteri was found as eudominant species; Philotarsus picicornis, Caecilius flavidus and Peripsocus subfasciatus were found as dominant species. In natural geobiocoenoses with the level of naturalness 1 or 2, the following species were found: Mesopsocus unipunctatus, Caecilius flavidus, and Caecilius burmeisteri as eudominant and Caecilius despaxi as dominant. Taxocoenoses of psocids were evaluated by Detrended Correspondence Analysis (DCA) and Divisive Cluster Analysis (DvClA). The axes were interpreted in DCA-analysis as follows: the x-axis denotes the influence of VTs and the q-axis refers to the influence of hydricity. This material was compared with other material obtained from various vegetation tiers in the Western Carpathians Mts. The characteristic species composition of psocids in the 5^th VT was as follows: Caecilius flavidus - C. burmeisteri - C. despaxi - Metylophorus nebulosus - Philotarsus picicornis.

The spruce-fir-beech dominated forest stands in Litschau in the Austrian part of the Bohemian Massif were converted by former forest management practices into pure Norway spruce stands and are now discussed to be reconverted into the potential natural vegetation type. The targeted potential natural vegetation type is usually defined by experts in vegetation sciences. Because meanwhile individual-tree growth simulators are a well acknowledged tool for predicting future forest stand development, in this study we investigate if PROGNAUS can also be used to predict the redevelopment of managed forest ecosystems into natural forest ecosystems regarding species composition. The development of 23 stands in Litschau has been simulated over 1,000 years under the "no-management" option. Generally, the simulated species distribution agrees quite well with the expectations of the potential natural vegetation type. However, the predicted amounts of silver fir and maple species are lower than expected, which probably is due to browsing and management effects represented in the parameterization data for PROGNAUS.

The impact of blue spruce (Picea pungens) and reed Calamagrostis villosa) cover on quantity and quality of upper soil layers was investigated. The research was conducted in the Jizerské hory Mts., Czech Republic (altitude 880 m, acidic spruce forest site type - 8K). Mean weight of dry matter of holorganic horizons was similar under both variants. Totally, there were accumulated 153 t/ha of dry matter of humus horizons in blue spruce and 174 t/ha in reed. Soil pH (KCl) varied from 3.7 to 3.2 under blue spruce stand and from 3.6 to 3.3 under reed. The differences of concentrations of nutrients (P, K, Ca, Mg) were not found significant either. Only L horizon showed significant differences: there were higher values of cation exchangeable capacity (T) and higher content of exchangeable bases (S) under reed. We found very similar forest-floor humus properties under both species. Therefore we can not state worsening of the soil conditions under blue spruce compared to areas covered with tested forest weed species.

Wild cherry is one of the noble hardwood species that increase the biodiversity of our forests and at the same time it could increase the income for forest owners. The preconditions for achieving these goals are the high quality of stem and appropriate silvicultural management. This means that wild cherry should occupy the main crown layer in the stand. The height/frequency diagram depicts two groups of wild cherry trees in the stand belonging to dominant/codominant and suppressed tree classes. Height periodic increment (measured between the years 2001 and 2007) is significantly (p < 0.01) different in these two groups confirming that there is no transition chance for the trees from the suppressed group to become a part of the main crown layer and play the role of future crop tree. The same is true of the diameter/frequency diagram which also has a two-peak shape remaining also at the end of the surveyed period. Our result suggests that silvicultural care should be focused only on trees belonging to future crop trees.

Effective forest management demands detailed knowledge of resources. Local forest users are usually the best source of information. In the Democratic Republic of Congo forest management partnerships between local people and government staff have become more common. A proper understanding of local perceptions of the forest is needed. This study was designed and guided by a multidisciplinary team including a sociologist, a linguist and a forester. It identified and compared classification and management units. It also touched on the different uses of the forest products, the species that provided these products and the locations of these species.

Multiaged silviculture is highly variable across North America but a commonality is the ties to the negative exponential diameter distribution to guide stocking control. These methods have evolved in several regions to include alternative stand structures and new stocking control tools are being developed. A trend in these new developments is integrating disturbance regimes and their effects on stand structure. The result, in some cases, is a movement towards longer cutting cycles and more flexible guidelines for stand structure.

A young speckled alder (Alnus incana [L.] Moench) stand was planted on a tract clear-felled due to air pollution and located on a summit plateau of the Jizerské hory Mts. (Central Europe, Czech Republic) at an altitude of 950 m a.s.l. The aim of the experiment was to test the suitability of Alnus incana to form preparatory stands covering the site and thus enabling the reintroduction of more sensitive target species. A potential of Alnus incana to respond to slow-release fertilizing was tested as well. The control treatment showed sufficient growth dynamics, nevertheless, the fertilization significantly promoted the growth (documented by height, height increment and stem-base diameter). If some limitations of alder such as high light requirements are respected, the speckled alder can be recommended as a suitable species for preparatory stands even in the 7^th and 8^th altitudinal (vegetation) zones, especially when fertilized.

High quality of planting material is an essential prerequisite for successful artificial forest regeneration. We carried out a detailed investigation aimed at differences between plantable bareroot and container plants of Norway spruce (Picea abies [L.] Karst.). Based on the results of this experiment, there exist marked differences in basic morphological traits between bareroot plants and plugs. The largest differences were observed in root collar diameter and root system volume. Differences in physiological quality (nutrient content, function of assimilatory organs) were also great. The results document that container seedlings of Norway spruce produced by intensive technology in controlled conditions of plastic greenhouses have very good predispositions for successful growth in difficult mountain conditions.

Geostatistical analysis of bark beetle activity and its use for the improvement of pest control measures are presented. Data on the volume of salvage timber felling due to bark beetle infestation for the period 2002-2004 were used for the analysis. Research was carried out in the northwestern part of Slovakia. The techniques used were variogram modelling, Turning Bands Simulation and selected techniques of simulation post-processing. The maps indicating the probability that a critical volume of timber felling was exceeded in a particular location were produced. The proposed system may be used effectively for the improvement of pest control measures and development of slow the spread strategies. The benefits and shortcomings of this approach in forest protection are discussed.

The aim of the study is to evaluate the role of the Grand fir (Abies grandis [Dougl. ex D. Don] Lindl.) as a soil-improving species in the area of the University Training Forest in Kostelec nad Černými lesy, Jevany district. The state of humus forms in the stand part with Grand fir was compared with the mature Norway spruce (Picea abies [L.] Karst.) without regeneration, with young beech (Fagus sylvatica L.), Norway spruce (Picea abies [L.] Karst.) and oak (Quercus spp. L.) stands (all of pole stage). The site is characterized as 4P1 - acid oak-fir site with Luzula pilosa, the geological bedrock is formed of cretaceous sandstone with loess overlays, the soils are Luvisols, the terrain is flat at the altitude of 420-440 m a.s.l. The humus form samples (L, F, H, Ah horizons) were taken in 4 replications, quantitatively for the holorganic layers. The dry mass amount and total nutrient contents were analyzed for holorganic horizons, the basic pedochemical characteristics (pH, soil adsorption complex characteristics, exchangeable acidity and exchangeable nutrients) were determined for all horizons. The results confirmed marked and positive effects of the Grand fir litter on the surface layer characteristics. This tree species supports the litter of good composition, transforming easily and forming humus forms of higher quality compared to coniferous as well as studied broadleaved species.

For the first time in the Czech Republic, bacteria identified as Pseudomonas marginalis, Pectobacterium carotovorum subsp. carotovorum and Pseudomonas putida were isolated from tubers of Zantedeschia spp. with symptoms of tuber soft rot. The symptoms occurred on mother tubers as well as on new daughter tubers of different calla lily hybrids with yellow spathe, calla lily cv. Mango with bright orange spathe and Zantedeschia rehmanii with pink spathe. The percentage of diseased plants of the total plants in the plot was around 10%. When inoculated into potato tuber slices, strains of P. marginalis and P. c. subsp. carotovorum produced soft rot. Pectolytic activity of P. marginalis strains was less intensive than that of the P. c. subsp. carotovorum strain. The results confirm that bacterial soft rot of Zantedeschia spp. may have several causes.

A downy mildew was observed on Buddleja × weyeriana Weyer ex Rehd. (B. davidii × globosa) cv. Sungold in a nursery in the northern part of the Czech Republic in July 2008. This downy mildew disease of Buddleja (Butterfly Bush) had been unknown in the country so far. Characteristics of visual symptoms and microscopic features are described. The morphology of the fungus was typical for Peronospora hariotii, conforming to the published description by GÄUMANN (1919). The sexual stage was not found. This is the first report of Peronospora hariotii causing leaf spot disease on Buddleja in the Czech Republic.

We conducted a pot experiment to evaluate the Cd and Zn accumulation in leaves and roots of Salix smithiana (BOKU-03DE-001) and S. caprea (BOKU-01AT-004) clones grown on a metal-contaminated soil as affected by native microbes extracted from the same experimental soil, and the fungus Cadophora finlandica. Plant biomass production of S. smithiana was decreased in all the treatments compared to the sterilized control. In contrast, S. caprea grew best on the non-sterilized soil. Similar effects were observed for plant Zn and Cd contents. Microbial treatments affected metal accumulation differently in the two Salix species. The effects of the microbial treatments on biomass and metal content of leaves were not related to the degree of mycorrhization. A comparison with literature data suggests that the plant response to microbial inoculation in terms of metal accumulation may depend on the plant-internal metal concentration. Our findings also illustrate a difficulty of successful rhizosphere management using metal-tolerant microbial isolates to further enhance the phytoextraction process.

Significant quantities of Cd have been added to soils globally due to various anthropogenic activities, posing a serious threat to safe food production and human health. Rhizosphere, as an important interface of soil and plant, plays a significant role in the agro-environmental system. This article presents a review of relationship between root excretion and microorganisms and plant resistance to Cd toxicity and possible mechanisms. Root exudates markedly altered in species and quantity under Cd stress. Root exudates can affect Cd absorption by plants through changing the physical and chemical characteristics of rhizospheres. The influence of root exudates on Cd bioavailability and toxicity may include modifying the rhizosphere pH and Eh, chelating/complexing and depositing with Cd ions, and altering the community construction, the numbers and activities of rhizospheric microbes. In this paper, the methods to reduce the transfer of Cd in soil-plant system by adjusting rhizosphere environment are discussed, and some aspects are also proposed that should be emphasized in the future research work.

Sequential extractions, metal uptake by Taraxacum officinale, Ruby's physiologically based extraction test (PBET) and toxicity characteristic leaching procedure (TCLP), were used to assess the risk of Pb and Zn in contaminated soils, and to determine relationships among soil characteristics, heavy metals soil fractionation, bioavailability and leachability. Regression analysis using linear and 2nd order polynomial models indicated relationships between Pb and Zn contamination and soil properties, although of small significance (P < 0.05). Statistically highly significant correlations (P < 0.001) were obtained using multiple regression analysis. A correlation between soil cation exchange capacity (CEC) and soil organic matter and clay content was expected. The proportion of Pb in the PBET intestinal phase correlated with total soil Pb and Pb bound to soil oxides and the organic matter fraction. The leachable Pb, extracted with TCLP, correlated with the Pb bound to carbonates and soil organic matter content (R² = 69%). No highly significant correlations (P < 0.001) for Zn with soil properties or Zn fractionation were obtained using multiple regression.

Fast-growing woody plants that can be grown under short-rotation systems offer an alternative to food production on arable land, and serve as a potential source of renewable energy. In order to establish the feasibility of future large scale production under the conditions of the Czech-Moravian highland, a high density experimental field plantation including a range of available clones of Populus sp. and Salix sp. with the total area of 1.5 ha was established in early 2001 in Domanínek (Czech Republic, 49°32'N, 16°15'E and altitude 530 m). The clone experiment of Populus sp. covered 0.3 ha in the center of the plantation and included 13 clones in total, with hardwood cuttings of only 6 clones available in numbers allowing 4-replicate experiment. The plantation was established on agricultural land and the trees were planted in a double row design with a density of 10 000 trees/ha. The trial was weeded by mechanical methods, and no irrigation, fertilization, or herbicides were applied. The experiment site was harvested at the end of 2006. It was found that the biomass yields of the tested clones of Populus sp. were in the higher range of results from national and European studies in case of hybrid clones. The satisfactory survival rate in the first year, when mortality tends to be highest, was supported by relatively wet weather conditions after plantation establishment. At the end of the first rotation, the highest yields were obtained from clones J-105 and J-104 (P. nigra × P. maximowiczii) and P-494 (P. maximowiczii × P. berolinensis) with J-105 showing a mean annual increment of dry matter close to 14 t/ha. Additional experiments seem to suggest that well managed poplar plantation might produce even better values if higher survival rates can be achieved.

The effects of different concentrations of copper (0-800 μmol) on growth, protein contents, peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) in Jatropha curcas L. seedlings were assessed by means of pot experiments. Results suggested that increased copper concentrations lead to decreased shoot elongation and seedling biomass. Protein content in the leaves and roots reached their highest levels at the copper concentrations of 400 μmol, while the highest protein content in the stem was observed at 800 μmol copper. POD activity in leaves and stems was unaffected at low copper concentrations, but showed a considerable variation at high copper concentrations. In roots, the highest POD activity was observed at 200 μmol copper. Under copper stress, SOD activity in leaves increased concomitantly with increasing copper up to 400 μmol, and SOD activity in stems and roots showed a slight increase. Catalase activity significantly elevated in leaves and roots but showed no significant changes in stems of the seedlings exposed to copper. A gradual increase of PAL activity in leaves and roots at the copper concentration of 400 and 200 μmol was observed, while PAL activity remained unchanged in stems.

Pot experiments were conducted to determine the best time for application of (ethylenediaminetetraacetic acid) EDTA and sheep manure extract (SME) in phytoremediation of a contaminated soil by Helianthus annuus. The plant was grown in a mine calcareous soil treated with increasing concentrations of EDTA or SME in 30 and 10 days before sowing (T1 and T2) and 10 and 30 days after sowing (T3 and T4). The best time for EDTA application was T4. The EDTA application before seed germination significantly reduced sunflower seedling emergence and dry weight. Soil available Pb and lead concentrations in plant organs increased with EDTA concentration but the actual amount of phytoextracted Pb decreased at high EDTA concentrations significantly, due to severe growth depression. SME application after sowing can increase plant dry weight and Pb concentration in the soil solution, enhancing the accumulated metal concentrations in shoots and roots. However the results showed that the most efficient treatments for Pb phytoextraction by sunflower are applications of 0.5 and 2 g EDTA/kg soil at T3 and T4, respectively.

Soils were sampled from the plots with four variants of fertilisation: Nil - without fertilisation, NPK - mineral fertilisation, FYM - farmyard manure, FYM + NPK - farmyard manure with mineral fertilisation, and two variants of crop rotation: field IV - classical 9-year crop rotation, field B - 2-year rotation of alternative growing. Determination of urease, CFU of Azotobacter spp. and potential nitrogenase activity was conducted during the period 1999-2004. The urease activity was positively affected by manure fertilisation (FYM) and by the combination of FYM + NPK. The statistically significantly highest counts of Azotobacter spp. and the highest nitrogenase activity were determined on field B in variants FYM and FYM + NPK. The results show that there was a higher amount of accessible nitrogen present on field IV than on field B. This might explain the lower counts of Azotobacter spp. and therefore the lower nitrogenase activity. According to our results, activity of urease, CFU of Azotobacter spp. and potential nitrogenase activity are very closely connected with N inputs.

The aim of the paper was to contribute to the acquisition of background data for the specification of safe boron levels in soils in relation to diagnostics by multi-nutrient soil tests and to CEC (cation exchange capacity) value of soil, pH and soil organic matter. The research was conducted on 24 soils. Sorption was determined after 97 days from the application of B at the rates of 0, 1, 2.5, 5 mg B/kg in H₃BO₃. The closest regression of B-sorption was with the CEC value of soil in NH₄-acetate and water tests and it increased with the increasing application of B (regression at a rate of 5 ppm B; NH₄-acetate: linear R

This paper is aimed to investigate the effects of some terricolous lichens on soil bacteria's growth in natural conditions. It is focused on species of bacteria and also on numbers of colony of soil specimens that were taken from substrates of three different terricolous lichen species. Peltigera rufescens (Weiss) Humb., which has not secondary metabolites, did not show an inhibition effect on soil bacteria. However, Peltigera neckerii Hepp ex Müll. Arg., which has secondary metabolites, has a negative effect on soil bacteria's growth. Besides, it was observed that Cladonia rangiformis Hoffm., which has many kinds of secondary metabolites, has the highest inhibition effect among the studied species. For this reason, we think that in the next researches, it is convenient to investigate elaborately by soil analysis the effect of lichen's secondary metabolites that have an effect on soil mineralization, on soil bacteria's growth.

The objective of this study was to investigate the capability of radish to extract lead from soils contaminated with lead resulting from air pollution. A randomized block experiment design was performed. The soil was contaminated with PbNO₃ and the treatments consisted of 180 (standard), 250, 350, 450, 800 and 1000 mg/kg lead. After development, plants were harvested and divided into shoots and roots. The lead content of each plant part as well as the soil-lead were measured. The results indicated a non-linear positive relation between the lead concentrations in soil and that accumulated in plant roots and shoots. By increasing the lead concentration in soil, its accumulation in plant tissues was also increased. Most of the extracted lead was accumulated in the roots (208.1 mg/kg) compared to shoots (27.25 mg/kg). Since radish can be seeded up to five times a year, and its yield may reach up to 20 t/ha, it can be used to remediate lead-polluted topsoils (0-10 cm).

In this research cyanobacteria (Blue-Green Algae, BGA) were isolated, identified, multiplied and used as an inoculum in pot rice experiment. The pH, moisture and algal population were measured in four seasons. The highest and lowest pH (6.7, 6.2), moisture of soil (43%, 34%) and algal population (12, 20 Colony-Forming Units/50 ml on A and B medium and 4, 5 Colony-Forming Units/50 ml on A and B medium) were recorded in spring and winter, respectively. The only heterocystous cyanobacteria were found in soil samples identified as Anabaena with four species (A. spiroides, A. variabilis, A. torulosa and A. osillarioides). The germination of rice seeds treated with cyanobacteria was faster than control. The result of pot experiment were: increase of 53% in plant height; 66% in roots length; 58% in fresh leaf and stem weight; 80% in fresh root weight; 125% in dry leaf and stem weight; 150% in dry root weight; 20% in soil moisture; 28% in soil porosity and a decrease of 9.8% in soil bulk density and 4.8% in soil particle density. There were significant differences (P < 0.05) in pot treated with BGA as compared with control.

Plant root and shoot dry weight, leaf relative water content (LRWC) and chlorophyll content were reduced by 30%, 21%, 15%, 34%, respectively, at 40mM NaCl as compared to non-salt stress conditions. However, membrane permeability (MP) of plant increased (85.0%) with increasing salinity. Foliar nutrient application (FNA) alleviated deleterious effects of salinity stress on growth and this effect was statistically significant. The highest alleviation effect of FNA at 40mM salinity stress was observed in the case of 10mM foliar KNO₃ and Ca(NO₃)₂ application, resulting in increase in plant root dry weight (50%), shoot dry weight (50%), LRWC (8.2%) and MP decrease (27.4%) at 40mM NaCl. Phosphorus, Fe and Zn contents in shoots and roots of plants also increased with FNA treatments, but they were still much lower than those of non-salt stress treatment. Sulphur, P, Fe and Zn contents of shoots reached similar values as in non-salt stress treatment when KNO₃ was applied, whereas Fe, Mn, Zn, and Cu contents of roots reached the values of non-salt stress treatment when Ca(NO₃)₂ was applied.

Soil samples (from Czech and German long-term field experiments) were used to estimate different soil phosphorus (P) fractions. More than 200 topsoil (0-30 cm) samples from different fertilizing treatments were taken. These were analyzed for P in soil solution (P_CaCl2) [0.01M CaCl₂ extract], exchangeable sorbed P (P_ex) [anion exchange (AE) membranes] and bioavailable P [Doppel-Lactat and Mehlich 3 (P_DL and P_M3)]. Other fractions analyzed were total inorganic (P_in), total (P_M-tot) and organic (P_org) P [fractionation after Marks], P sorbed on Fe and Al (P_FeAl) [fractionation after Schwertmann] and residual P (P_ar) [aqua regia extract]. Comparison of medians appeared to be better for evaluating extraction abilities. Phosphorus fractions were in the following order: (P_ar = 100%); P_CaCl2 (0.2%) < P_ex (9%) < P_DL (10%) < P_M3 (16%) < P_in (24%) < P_org (37%) < P_FeAl (55%) < P_M-tot (59%). Low amounts of P_in, P_org and P_M-tot did not verify the applicability of the Marks' fractionation for the set of studied soils. Close correlations at P ≤ 0.001 were found for all methods for estimating the fractions of bioavailable phosphates (P_CaCl2, P_ex, P_DL and P_M3). Statistically significant relations were observed between P_in with P_ar, P_M-tot and P_FeAl.