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Status and trend analysis of the production, export and import of wood and wood products in the G20 countries from 2004 to 2021Original Paper

Vinod Kumar Upadhyay, Ritesh Tailor, Rashmi Ramesh Shanbhag, Narasimha Murthy, Pradeep Kumar Kushwaha, Manish Ranjan

J. For. Sci., 2025, 71(4):159-170 | DOI: 10.17221/42/2024-JFS

The aim of this work was to study the use of different types of wood products by the G20 countries. In the current scenario, every country is concerned about the severe exploitation of forests and the ensuing lack of markets for timber. Production and consumption of wood and wood products by the G20 countries account for 85% of global GDP and two-thirds of the world's population. Data for the study includes eight major categories of wood products in the G20 countries from 2004 to 2021: industrial raw wood, sawlogs and veneer logs, sawn wood, wood fuel, wood-based panels, paper and paperboard, pulp for paper, wood pellets, and other agglomerates. Autoregressive integrated moving average (ARIMA) models were used to predict the production, export, and import behaviour of wood and wood products from 2022 to 2030. According to the findings, the G20 countries would consume 1 644.6 million m3 of industrial raw wood in 2030, followed by 984.8 million m3 of sawlogs and veneer logs, 454.7 million m3 of sawn wood, 882.5 million m3 of wood fuel, 406.2 million t of paper and paperboard, 199.4 million t of pulp for paper, and 51.7 million t of wood pellets and other agglomerates. This study provides decision-makers with an analysis of the production and consumption of wood product markets in the G20 countries, which can be used for policy decisions.

Height growth of Quercus petraea coppices: Effects of tree type, stand management, and site conditions – A case study from the Czech lowlandsOriginal Paper

Jan Kadavý, Michal Kneifl, Barbora Uherková

J. For. Sci., 2026, 72(2):93-105 | DOI: 10.17221/7/2026-JFS


This study examines height growth and its driving factors in stools and standard trees, the two key structural components of coppice-with-standards stands. We focused on sessile oak [Quercus petraea agg. (Matt.) Liebl.] stands over 90 years old at two contrasting sites near Brno (South Moravian Region, Czech Republic): one actively managed and one left unmanaged for more than 40 years as a strict nature reserve. Tree heights were analysed using two-factor analysis of variance (ANOVA) with post hoc tests and multinomial logistic regression. A total of 1 239 trees were measured (584 stools and 655 standards). Standard trees were the tallest on average (21.8 m), significantly exceeding all the stool categories, whereas managed triple-stem stools were the shortest (15.97 m). Across all categories, unmanaged stands generally reached greater heights than managed stands did, except for standard trees. The forest type, exposure and slope had significant effects on height class probabilities, whereas elevation was important only for smaller trees. Overall, the results demonstrate that both the tree type and site conditions strongly influence height growth in Q. petraea coppice-with-standards stands, with management history leaving a clear biological legacy in the present-day stand structure.

Isolation of aerobic bacteria from surgical site infections following orthopaedic operations in cats and dogsOriginal Paper

A Gulaydin, O Gulaydin, MB Akgul

Vet Med - Czech, 2024, 69(7):243-253 | DOI: 10.17221/26/2024-VETMED

Surgical site infections (SSIs) cause significant complications in the postoperative period in veterinary surgeries. Determining the aetiology of infections is crucial for increasing treatment success rates. This study aims to assess treatment processes by identifying the bacterial agents responsible for infections occurring in cats and dogs after orthopaedic operations and to determine the antimicrobial resistance profiles of these agents. Strains isolated from SSIs were retrospectively analysed in patients brought to the Surgical Clinic of Siirt University Animal Health Application and Research Hospital between 2021 and 2023. The isolates were identified using MALDI-TOF MS. The disc diffusion method was applied to determine the antimicrobial susceptibility of the isolates. A high isolation rate was detected in methicillin-resistant Staphylococcus pseudintermedius and extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae. According to the antimicrobial susceptibility results, cephalosporin treatment was continued in only one of the cases in which prophylactic treatment with cephalosporin group antibiotics was applied. Consequently, this study revealed that preoperative prophylactic antibiotic administration may not be sufficient in preventing surgical site infections. Diagnosis of aetiological agents and evaluation of antimicrobial susceptibility are essential in preventing surgical site infections and determining effective treatment options.

Temporal variations in flowering of ‘Honeycrisp’ apple grafted on eight different rootstocksOriginal Paper

Oscar Cruz-Alvarez, José de Jesus Ornelas-Paz, Diana Laura Araujo-Pallares, María Nohemí Frias-Moreno, Graciela Dolores Ávila-Quezada, Damaris Leopoldina Ojeda-Barrios, Juan Luis Jacobo-Cuellar

Hort. Sci. (Prague), 2025, 52(3):211-223 | DOI: 10.17221/32/2024-HORTSCI

Malus domestica is one of the world’s most important deciduous fruit trees. Over a four-year period (2017–2020), temporal variations in flowering were evaluated in ‘Honeycrisp’ apple trees on eight rootstocks (G.30, G.969, G.202, G.41, G.11, M.9T337, M.26 EMLA and V.6) planted in 2014 in Chihuahua, México. Among the variables evaluated were the probability of late-spring frost, winter chill units, growing degree days, flowering period, foliar nutrient concentrations, trunk cross-sectional area, number and weight of fruit per tree, and production efficiency. Significantly different chill unit accumulations occurred over the four years, with values falling between 974 and 1 415, where for the latter value, the start of flower opening was earlier, but there was a higher risk of damage by temperatures ≤ –2 °C. There was no effect of rootstock on the time of onset and end of flowering. The most productive combinations were ‘Honeycrisp’ on G.969, G.11 and V.6 with yield estimates of 35 300, 34 200 and 33 600 kg/ha, respectively. The commercial production of ‘Honeycrisp’ apple trees requires the evaluation of their agronomic performance with different rootstocks. Flowering is particularly important since this phenological stage is so closely linked to productivity and is strongly affected by variations in winter temperatures.

Research on an effective artificial regeneration method for selected commercially important coniferous tree species on a large sanitation cut site situated in the Javorníky Mts., Western CarpathiansOriginal Paper

Martin Belko, Anna Tuèeková, Roman Longauer, Valéria Longauerová, Vladimír Maèejovský, Ivan Horvát

J. For. Sci., 2024, 70(7):353-367 | DOI: 10.17221/1/2024-JFS

In response to the decline of Central European spruce monocultures driven by various factors, the Demonstration Object of Reconstruction of Spruce Forests (DORS) was established in Husárik locality, Javorníky Mts., northwestern Slovakia. The area includes the Husárik trial site, where the applicability and efficiency of different artificial regeneration methods are studied. The trial was established on a 24-ha area cleared following the outbreak of spruce bark beetles in 2011. Its altitude is 800 m a.s.l., aspect NW, slope 30%, the soil is Ranker on the soft flysch sandstone bedrock. Our study covered 4 conifers – Norway spruce (spruce), European larch (larch), silver fir (fir), and Douglas fir (doug fir). Each species was regenerated using 4 different approaches: planting of commercial bareroot transplants (BR), planting of container transplants (CON), direct seeding (DS) and vegetative cell seeding using seed shelters (VCS). Results concerning the nine-year development of transplants and seedlings, along with the calculation of cost-efficiency, are presented. As to the species, BR and CON transplants of spruce and larch reached the best survival and height. The DS larch was the most cost-efficient method of establishment of a successfully established plantation (survival > 50%; stem height > 2/3 of the weed height; ratio of damaged individuals < 50%) with a total cost of 2 372 EUR·ha–1. On the contrary, the slow initial growth of fir and Douglas fir and their extensive damage resulted in the incomparably higher cost of establishment of their successfully established plantation, such as 4 980 EUR·ha–1 for five-years-old BR fir transplants. Our findings documented that current efforts related to the restoration of salvage-felled clearings remained difficult, especially in the case of introduction or reestablishment of coniferous tree species more vulnerable to open site conditions.

The effect of mechanical site preparation on sandy soil properties in Scots pine plantationsOriginal Paper

Ján ®idó, Milan Ka¹iar, Marián Homolák, Erika Gömöryová

J. For. Sci., 2024, 70(11):593-601 | DOI: 10.17221/54/2024-JFS

Sandy soils represent an extreme environment for tree growth. Traditionally, site preparation before planting involves removing logging residues (LRR) and ploughing. An alternative method is incorporating logging residues (LRI) into the topsoil which may enhance tree regeneration and seedling growth. The aim of this study was to assess whether and how different site preparation techniques affect soil physico-chemical and microbial properties over the long term. The study was performed in the Záhorská ní¾ina lowland (Slovakia) in September 2020. Soil samples were taken in two 25-year-old Pinus sylvestris (L.) plantations along five soil profiles in each stand, down to a depth of 30 cm. Results showed a significant increase in carbon and nitrogen concentration and soil moisture in the LRI plot. However, soil pH and phosphorus content significantly decreased. No significant differences were observed in calcium, magnesium, and potassium concentrations between the differently treated plots. The LRI plot also exhibited a significant increase in microbial biomass carbon, N-mineralisation, and catalase activity. The results indicate that different mechanical site preparation methods may impact soil properties over the long term, likely through improved seedling survival and tree growth.

European rose chafer prevalence on cultivated highbush blueberriesOriginal Paper

Maxhun Shehaj, Erzen Shehaj, Elbasan Çekaj, Agrinë Naqa, Blerinë Krasniqi

Hort. Sci. (Prague), 2025, 52(2):155-161 | DOI: 10.17221/28/2024-HORTSCI

The purpose of this study was to detect the prevalence rate of European rose chafer (Cetonia aurata) on American highbush blueberries (Vaccinium corymbosum). The observation was made from the beginning of flowering to the end of harvest of the blueberry cultivars ‘Duke’, ‘Legacy’, and ‘Huron’. The incidence occurs during the first harvest of the ‘Duke’ cultivar. Three rows per cultivar, with 90 plants per row, were monitored during the research. Insects were collected on 15 infested plants per row, randomly selected, and counted. This procedure was repeated every second day, 3 times a day: in the morning, at noon, and in the afternoon. The highest presence of the insect was recorded at the full ripening phase of the ‘Duke’ cultivar, with 25.5% of bushes being infested and an average of 12.7 beetles per bush counted. The damage percentage varied over the harvesting periods: 29% of the fruits were damaged in the first harvest, 13% in the second harvest, and 8% during the third harvest. While ‘Legacy’ and ‘Huron’ stood out with 5.55% and 3.33% of infected bushes, respectively, and minor fruit damage. This research proves that the European rose chafer in the area of the experiment is a harmful pest of early cultivated blueberry cultivars.

Knowing your enemy before taking the field: A screening of salt and UV-B treatments to boost the biochemical defences of tomato (Solanum lycopersicum L., ‘Moneymaker’ cultivar) plantlets in controlled conditionsOriginal Paper

Maria Calogera Sciampagna, Alessia Mannucci, Salvatore Limpido, Annamaria Ranieri, Antonella Castagna, Marco Santin

Hort. Sci. (Prague), 2025, 52(4):353-362 | DOI: 10.17221/109/2023-HORTSCI


Plants are commonly exposed to different abiotic stress factors that can threaten their growth and development. Abiotic treatments might be used as a tool to increase the tolerance of plants grown in controlled environments before being transplanted in open fields, but this technique needs a careful choice of the stressor dose. We screened several UV-B radiation doses (1 h, 3 h, 6 h and 8 h per day) and NaCl doses (75 mM, 150 mM, 300 mM), separately, to be employed as preconditioning treatments of tomato plantlets (Solanum lycopersicum L., ‘Moneymaker’ cultivar) cultivated in a controlled environment. Biometric parameters and antioxidant compounds were quantified in leaves and roots. Our results showed that the application of mild abiotic treatments such as 75 mM NaCl for 14 days or 8 h/day UV-B (18.43 kJ/m2) for 7 days to hydroponically grown tomato plantlets can increase the content of foliar total phenolics and flavonoids without inducing strong impairments in the plant growth and development. Interestingly, this work provides evidence that the UV-B radiation, although perceived only by the above-ground organs of the plants, also induces some morphological and biochemical changes in the root system.

Advancements in fuzzy expert systems for site-specific nitrogen fertilisation: Incorporating RGB colour codes and irrigation schedules for precision maize production in BangladeshOriginal Paper

Bitopi Biswas, Mohammad Tariful Alam Khan, Mohammad Billal Hossain Momen, Mohammad. Rashedur Rahman Tanvir, Abu Mohammad Shahidul Alam, M Robiul Islam Islam

Res. Agr. Eng., 2024, 70(3):155-166 | DOI: 10.17221/35/2024-RAE


The research was conducted at the Department of Agronomy and Agricultural Extension, Rajshahi University, from December 2021 to April 2022. The objective was to develop a fuzzy expert system for site-specific N fertilisation using leaf colour code (RGB) and irrigation frequencies for maize yield. The experiment encompassed two primary factors: nitrogen fertiliser application rates (N1: 100%, N2: 75%, N3: 50% of conventional rates) and irrigation frequencies (I1: 100%, I2: 75%, I3: 50% of pan evaporation). A completely randomised design (CRD) with three replications was used to arrange the experimental pots, each receiving recommended doses of phosphorus, potassium, and sulfur, with urea applied per treatment instructions. Results revealed significant chlorophyll content and grain yield differences among the various nitrogen fertiliser rates. The highest grain yield (219.27 g·pot–1) was observed with N1, whereas the lowest (186.6 g·pot–1) was with N3. Similarly, irrigation frequencies significantly influenced chlorophyll content and cob characteristics, with I1 resulting in the highest grain yield (211.27 g·pot–1) and I3 the lowest (184.6 g·pot–1). Furthermore, the interaction between fertiliser application rates and irrigation frequencies had notable effects on various parameters, leading to the highest grain yield of 227.62 g·pot–1 with the combination of N1 and I1 and the lowest (168.00 g·pot–1) with N3 I3. The agricultural experiments were facilitated using the Matlab fuzzy toolbox, employing the Mamdani inference method. Fuzzy rules were delineated for nitrogen application rates and irrigation frequencies, with three fuzzy sets each. Membership functions were developed utilising Matlab's fuzzy interface system (FIS) editor and membership function editor, optimising leaf chlorophyll content, evaporation rate as input tiger N fertilisation, and irrigation frequencies as output for precise maize production in Bangladesh.

A study on effects of rootstocks on vine balance of 'Cerason' cultivarOriginal Paper

Jiøí Tethal, Jiøí Sochor, Radek Sotoláø, Pavel Pavlou¹ek, Mojmír Baroò, Richard Danko, Magdalena Kap³an, Kamila Klimek

Hort. Sci. (Prague), 2025, 52(4):293-303 | DOI: 10.17221/16/2025-HORTSCI


‘Cerason’ is an interspecific cultivar of grapevine that has been bred and selected in the Czech Republic (2008). The cultivar is a result of crossing the cultivars ‘Merlan’ and ‘Fratava’ and shows very promising potential within the framework of a fungus-resistant (PIWI) cultivar, making it particularly suitable for organic wine-making. This paper presents the results of an experiment performed with seven selected rootstock cultivars (viz. ‘Kober 125AA’, ‘Amos’, ‘Börner’, ‘Crâciunel 2’, ‘Kober 5BB’, ‘K1SO4’ and ‘Teleki 5C’) and some data about the effects of these rootstocks on the quantitative variables of the cultivar ‘Cerason’. The aim of this study was to choose and specify the rootstock cultivars that would be the most suitable for a given locality. The following traits were monitored and recorded: plant vitality, the yielding capacity of the individual plants, and the number and weight of clusters per vine. The weight of 100 berries was also an important variable. The rootstock cultivars ‘Kober 125AA’ and ‘Kober 5BB’ were evaluated as the best for optimal growth and fertility (especially a higher fertility index, pruning weight and weight of clusters). The experimental results indicated that the cultivar ‘Crâciunel 2’ could also be a suitable rootstock. The ‘Kober 125AA’ rootstock also showed the most optimal Ravaz index, with an average of 5.9 over the three-year observation period.

Effect of ripening temperature on early-season ‘Hass’ avocado fruit exocarp colour development and pigmentation during ripeningOriginal Paper

December Simphiwe Sibeko, Kingsly Shikwambana, Nhlanhla Mathaba

Hort. Sci. (Prague), 2024, 51(2):152-159 | DOI: 10.17221/72/2023-HORTSCI

South African ‘Hass’ avocado fruit harvested early season are vulnerable to colour desynchronisation with softening during ripening, reducing their international and local market aesthetic value. So far, research has proven that ‘Hass’ avocado fruit exocarp colour desynchronization is an early season conundrum. However, there is insufficient literature on underlying factors causing exocarp colour desynchronisation. Therefore, the study aimed to investigate the ripening temperature effect on early season ‘Hass’ avocado fruit exocarp colour development and pigments during ripening. Early season ‘Hass’ avocado fruit were stored at 5.5 °C for 28 days thereafter, ripened at 16, 21 and 25 °C and evaluated for firmness, visual and objective colour (L*, C* and h°), total chlorophyll and anthocyanin content. The results showed that the visual colour of fruit ripened at 25 °C was significantly higher compared to 21 and 16 °C after 2 and 4 ripening days. This study found that ripening temperature had no significant influence on chlorophyll degradation. However, fruit ripened at 25 °C accumulated significantly higher exocarp anthocyanin concentration compared to lower ripening temperatures. In conclusion, ripening early season ‘Hass’ avocado fruit at 25 °C resulted in improved exocarp colour development and anthocyanin accumulation.

Selection of trees for rubbing by the wild boar (Sus scrofa) in the Sidi Boughaba forested Moroccan Ramsar site: Assessment, implications, and perspectivesOriginal Paper

Abdellah Ichen, Saâd Hanane, Mohammed Bouaamama, Mohamed Alaoui, Najib Magri, Abdelaziz Benhoussa

J. For. Sci., 2023, 69(1):11-20 | DOI: 10.17221/139/2022-JFS


Knowledge of mechanisms by which large mammals select rubbing trees (RT) is a major challenge for the effective management of forests and wildlife resources. In this study, we investigated this issue regarding the Wild boar (Sus scrofa) in the Moroccan forested site of Sidi Boughaba as a case study. We used data from four sets of variables, namely topography, forest type, landscape composition, and microhabitat, measured at 58 rub and control trees, to determine the factors associated with the occurrence of RT by means of generalized linear mixed models. Our results showed that the RT occurrence increased with a high density of red juniper trees and declined with distance to the nearest footpath. Variation partitioning analysis revealed that the pure fraction of microhabitat was the most robust in explaining this occurrence (adj. R2 = 0.17, P < 0.001), followed by that of forest type (adj. R2 = 0.05, P < 0.05). A scientific monitoring system must be set up to strike a balance between the availability of forest trees on the one hand and the pressure exerted by wild boars on this internationally importance site on the other. It is imperative to test the geographical generality of our results in other Mediterranean forests.

Organic versus conventional ‘Willamette’ raspberry: yield, bioactive compounds and antioxidant propertiesOriginal Paper

Senad Murtiæ, Milena Ðuriæ, Jasmin Fazliæ, Fikreta Behmen, Sanel Haseljiæ, Amina ©erbo, Haris Berbiæ

Hort. Sci. (Prague), 2023, 50(4):275-282 | DOI: 10.17221/164/2022-HORTSCI

This paper presents the results of a two-year trial on the yield, total phenolic and flavonoid contents and total antioxidant capacity of the ‘Willamette’ red raspberry under organic and conventional farming. A trial was conducted in a commercial plantation of ‘Willamette’ raspberries located in the southwestern part of Serbia. The total phenolics and flavonoids in the raspberry fruits were determined by the Folin-Ciocalteu reagent and aluminium chloride assay, respectively. The ferric reducing antioxidant power assay was used to evaluate the total antioxidant capacity of the raspberry fruits. The results of this study showed that the raspberry yields were significantly affected by the different farming systems. The raspberry yield in conventional farming was higher than that of organic farming. Contrastingly, the raspberries from the organic farming contained higher levels of the total phenolics and flavonoids and also had a higher total antioxidant activity compared to those from the conventional farming in both 2021 and 2022. This study also showed that the total phenolic and flavonoid contents of the ‘Willamette’ red raspberry have a significant and positive correlation with their total antioxidant capacity, indicating that the phenolic compounds play an important role in the antioxidant activity of the raspberry.

Effects of abscisic acid and gibberellin on sugar accumulation in ‘Fengtang’ Plum (Prunus salicina LindI.)Original Paper

Qianjun Song, Xiaoshuang Nie, Hong Chen

Hort. Sci. (Prague), 2024, 51(4):314-326 | DOI: 10.17221/25/2024-HORTSCI

To investigate the regulation mechanism of exogenous plant growth regulators on the sugar accumulation of ‘Fengtang’ plum fruits, and to provide theoretical support for improving the sugar content of ‘Fengtang’ plum fruits. The expression of fruit sugar fractions and related genes was determined by spraying different concentrations of exogenous  abscisic acid (ABA) and Gibberellic acid 3 (GA3) during the critical period of sugar accumulation in ‘Fengtang’ plum. The results showed that exogenous ABA treatment increased fruit soluble solids content, delayed the decline in fruit firmness, increased fruit sucrose and sorbitol content and decreased glucose and fructose content at 90 and 100 days after flowering, while exogenous GA3 treatment decreased fruit sorbitol and sucrose content and increased glucose and fructose content at 110 days after flowering; Exogenous ABA treatment significantly increased the expression of the fruit sugar transporter protein genes PsSWEET4 and PsSTP1 as well as the sucrose phosphate synthase genes PsSUS4 and PsSPS2 at 90 and 100 days after flowering, whereas exogenous GA3 treatment increased the expression of the neutral converting enzyme genes PsNINV1/3/4 at 90, 100, and 110 days after flowering to convert sucrose to fructose and glucose. The conclusion is that ABA increases fruit sugar content by increasing the expression of the fruit sugar transporter protein genes PsSWEET4 and PsSTP1 as well as the sucrose synthase genes PsSUS4 and PsSPS2, whereas GA3 decreases sugar accumulation and delays fruit ripening by decreasing the accumulation of sugar during ripening by increasing the expression of the neutral transforming enzyme genes PsNINV1/3/4 to break down sucrose into fructose and glucose.

Effects of sodium selenate and selenomethionine on reproductive growth, fruit quality, and 5-hydroxytryptophan metabolism of ‘Qingcui’ plumOriginal Paper

Sun Xieping, Chen Yang, Lin Ling Kou, Chun Xia Lu, Guo Qiang Han

Hort. Sci. (Prague), 2024, 51(2):141-151 | DOI: 10.17221/45/2023-HORTSCI

Selenium (Se) is a beneficial element for plant growth and development. In this study, three-year-old potted ‘Qingcui’ plums were treated with Na2SeO4 (Se6+) or selenomethionine (SeMet Se2-) to explore the effect of Se on the plant growth, fruit quality, and 5-hydroxytryptophan (5-HTP) metabolism. Flower and fruit numbers, fruit quality and yield, Se content, and 5-HTP metabolites and enzymes were detected. The results showed that the flower and fruit numbers, and yield were significantly increased by the Se application. There were no significant differences in the fruit diameters, fruit mass, edible part ratio, titratable acids, water content, and solid acid ratio among the treatments. The total soluble solids, soluble protein, and malondialdehyde contents under the Se6+ treatment showed no significant difference compared to the Se2- treatment, but they were significantly higher than these under control by 16.71%, 39.13%, and 36.27%, respectively. The Se application markedly increased plant the Se content, and Se contents in the roots and leaves, or the fruits were significantly larger by the Se6+ treatments than the Se2- treatment. The leaves’ pigment contents under the Se2- treatments were significantly larger than those under the control or Se6+ treatment. Tryptophan was not significantly influenced, the 5-HTP and 5-methoxytryptophan contents were reduced in the roots, and increased in the leaves, and the serotonin content was only significantly increased in the roots by the Se treatments. The tryptophan hydroxylase and hydroxyindole-O-methyltransferase levels were slightly influenced, and the tryptophan decarboxylase level in the roots or fruits was significantly increased by the Se treatments. The Se application had beneficial effects on the plant growth, fruit quality, and Se content, especially in the Se6+ treatment, and influenced the 5-HTP metabolism.

Colonization of ‘Sampion’ apple tree roots and rhizosphere by mycorrhizal fungi following the application of AquaGel or organic compost enriched with beneficial microorganismsOriginal Paper

Edyta Derkowska, Beata Sumorok, Lidia Sas-Paszt, Krzysztof Górnik, S³awomir G³uszek, Anna Lisek, Augustyn Mika, Mateusz Fr±c

Hort. Sci. (Prague), 2023, 50(4):253-261 | DOI: 10.17221/1/2023-HORTSCI

Apple trees of the cultivar ‘Sampion’ were planted at the Experimental Orchard of National Institute of Horticultural Research in D±browice. In a three-year experiment (2019–2021) the following fertilization combinations were applied: AquaGel (commercial hydrogel), AquaGel combined with a consortium of beneficial bacteria, AquaGel combined with a consortium of beneficial filamentous fungi, organic compost alone, compost combined with a consortium of beneficial bacteria, and compost combined with a consortium of beneficial filamentous fungi. The presence of arbuscular mycorrhizal fungi was assessed both in the rhizosphere soil and in the roots of apple trees. The results of the study indicate the most advantageous effect of compost combined with bacteria or fungi on the degree of root colonization by arbuscular mycorrhizal fungi and the formation of their spores in the soil.

Evaluation of five rootstocks on the growth, gas exchanges and chlorophyll fluorescence of Juglans regia L. cv. ‘Xiangling’Original Paper

Bing Hua Liu, Liang Cheng, Ji Xia Zhang, Lian Jia Yui, Hai Lin Ma, Fangchun Liu

Hort. Sci. (Prague), 2023, 50(3):208-218 | DOI: 10.17221/152/2022-HORTSCI

Our study comparative analyzed the morphological and physiological performance of Juglans regia L. cv. ‘Xiangling’ (JRX) grafted onto five rootstock species originated from China. A wide range of coefficient of variation (CV), 1.155–39.848%, was detected for fourteen indexes among the studied seedlings. CV of plant height, total dry biomass, total leaf area, root/shoot ratio, chlorophyll index, and stomatal conductance was higher than 15.00%, suggesting higher variation. Principal component analysis, hierarchical cluster analysis and subordinate function analysis were used to screen engrafted seedlings for their quality. All three methods produced the same result that seedling quality of ‘Xiangling’ engrafted with the five rootstock species from high to low were – Juglans regia L. cv. ‘Jizhaomian’ (JRJ), JRX, Juglans regia L. cv. ‘Lvxiang’ (JRL), Juglans mandshurica, Maxim., and Juglans hopeiensis Hu.. The evaluation results were highly consistent with the actual growth performance of the engrafted seedlings, it verified the reliability of our results and the evaluation methods. The results demonstrated that JRJ, JRX, and JRL are potential rootstock candidates in the studied walnut planting regions. It would provide important implication for the selection of proper rootstock species for a certain particular walnut variety.

First cases of herbicide resistance of Tripleurospermum inodorum in the Czech RepublicOriginal Paper

Jaromír ©uk, Jakub Mikulka, Madhab Kumar Sen, Pavlína Ko¹narová, Kateøina Hamouzová, Josef Soukup

Plant Soil Environ., 2023, 69(2):81-87 | DOI: 10.17221/427/2022-PSE

Tripleurospermum inodorum (L.) Sch. Bip. is one of the most economically important and yield-reducing weeds in cereals in Europe. Random and systematic monitoring of this weed might provide an early warning for the farmers and slow down the pace of the evolution of herbicide resistance. This study aimed to identify resistant populations of T. inodorum in the Czech Republic and elucidate their possible resistance mechanism/s. Monitoring and screening of T. inodorum for herbicide resistance against acetolactate synthase (ALS) inhibitors and synthetic auxins was carried out. Greenhouse experiments and molecular-genetics studies were conducted to characterize the resistance. While all the tested populations were found to be susceptible to synthetic auxins, two populations (MATIN 2 and MATIN 4) were found to be resistant against tribenuron (ALS inhibitor). However, their cross-resistance to florasulam was not confirmed. The resistance mechanism detected was the target-site substitution of Pro197 at the ALS gene. The two populations carried different point mutations: Pro197Ser (MATIN 2) and Pro197Gln (MATIN 4). This is the first study in the Czech Republic to identify the survival mechanism in T. inodorum for resistance to ALS-inhibiting herbicides. Our results from this research will provide basis for resistance management in T. inodorum in the Czech Republic and other countries.

The potential of glyphosate-alternatives like electrophysical weeding in the stale seedbed method for Alopecurus myosuroides (Huds.) controlOriginal Paper

Benjamin Klauk, Jan Petersen

Plant Soil Environ., 2023, 69(11):522-531 | DOI: 10.17221/386/2023-PSE


Changing political demands requires the search for alternatives to glyphosate, which has often been used in the stale seedbed method. In three field trials between 2020 and 2023, three electrophysical treatments (XPower System, Zasso®) differing in speed, three mechanical treatments (disc harrow, rotary harrow, cultivator) and three chemical treatments (glyphosate, maleic hydrazide, pelargonic acid + maleic hydrazide) were evaluated concerning the efficacy and economic performance in stale seedbed method in Alopecurus myosuroides control. Process costs for each treatment were calculated. Furthermore, the maximum investment costs for the XPower system were calculated to be on the same level as the other treatments. In all treatments, the density of A. myosuroides in autumn was significantly lower than in the control. In general, the fewest plants and heads were found in the chemical treatments. Despite the lower plant density, the electrophysical method did not show a significant difference in the number of heads compared to the untreated control, especially at higher speeds. Calculated process costs ranged between
40 €/ha (glyphosate) and 430 €/ha (pelargonic acid + maleic hydrazide). To be economically comparable with the other treatments, the investment of the XPower should be at maximum between – 219 000 € (glyphosate) and 300 000 € (pelargonic acid + maleic acid), depending on the driving speed. In a holistic view, electrophysical control, as well as pelargonic acid and maleic hydrazide as glyphosate alternatives in the stale seedbed method, are not suitable. A stale seedbed with mechanical control of A. myosuroides is recommended.

Characterisation of iodo- plus mesosulfuron resistance in an Alopecurus myosuroides Huds. Population from the Czech RepublicOriginal Paper

Kateøina Hamouzová, Jakub Mikulka, Jaromír ©uk, Madhab Kumar Sen, Pavlína Ko¹narová, Soham Bhattacharya, Josef Soukup

Plant Soil Environ., 2023, 69(12):608-614 | DOI: 10.17221/377/2023-PSE

The intensification of Alopecurus myosuroides Huds. (black-grass) is becoming a major problem due to its growing resistance to a broad spectrum of acetolactate synthase (ALS)-inhibiting herbicides. Hence, the present study was conducted to evaluate the resistance level of a black-grass population to iodosulfuron plus mesosulfuron and to identify the underlying resistance mechanism. Dose-response studies revealed that the resistance population is 22 times less sensitive to iodosulfuron plus mesosulfuron than the susceptible population. The probable resistance mechanism identified was the target-site substitution of proline (Pro) by threonine (Thr) at the 197th position of the ALS enzyme. Furthermore, whole plant response bioassay experiments demonstrated that this population is also resistant to pinoxaden, chlorotoluron, diflufenican plus pendimethalin plus chlorotoluron, fenoxaprop and flufenacet plus diflufenican. In summary, the current findings recommend using alternative herbicides in integrated weed management to interrupt the possible evolution of herbicide resistance in these species.

Scots Pine (Pinus sylvestris L.) establishment success under climate change: Effect of site, stock type and planting timeOriginal Paper

Anna Plaèková, Zdenìk Vacek, Stanislav Vacek, Jan Cukor, Josef Gallo, Jakub Èerný

J. For. Sci., 2025, 71(11):555-564 | DOI: 10.17221/84/2025-JFS

Scots pine (Pinus sylvestris L.) is one of the most widespread and economically important coniferous species in the Northern Hemisphere. However, its regeneration success has recently been increasingly affected by global climate change (GCC), particularly through increasing mortality. This research aimed to evaluate the early establishment success of 30 400 bare-root and containerised Scots pine seedlings and saplings of different height classes, planted at various times on acidic and gleyed sites (479–610 m a.s.l.) across four locations in the Czech Republic. On acidic sites, seedlings exhibited 16% higher height growth and 11% lower mortality compared to gleyed sites. Containerised planting stock achieved significantly (P < 0.05) greater height growth (by 83%) and lower mortality (by 36%) than bare-root stock. For containerised material, spring plantings showed superior growth and vitality compared to autumn plantings. Planting stock height class had a stronger influence on height growth than on mortality. Based on the results, planting containerised saplings of 26–35 cm or 36–50 cm height in April is recommended for optimal establishment success of Scots pine under GCC.

Modulating Norway spruce growth and resilience through thinning intensity under climate change conditionsOriginal Paper

Petra Jablonická, Pavel Horák, Jakub Èerný

J. For. Sci., 2025, 71(10):482-500 | DOI: 10.17221/55/2025-JFS

In recent decades, Norway spruce (Picea abies) stands have become increasingly vulnerable to frequent droughts and associated outbreaks of secondary biotic pests, resulting in significant degradation of forest ecosystems. To preserve their production and ecological functions, it is necessary to apply well-adapted silvicultural practices that mitigate the risk of stand decline. This study examines the effects of two thinning intensities (moderate and heavy) on stand productivity and resilience under varying site conditions. Three long-term research sites with paired differently thinned plots located within and outside the natural range of Norway spruce in the Czech Republic were analysed. Tree-ring width measurements were used to calculate radial growth trends and four resilience indices (resistance, resilience, recovery and average relative growth reduction). Across sites Blaník (BL), Tetøeví Boudy (TB) and ®elezná Ruda (ZR), basal area increment (BAI) differed significantly between thinning intensities (BL: P = 0.044; TB: P = 0.0076; ZR: P < 0.001), with moderate thinning showing higher BAI at BL and TB, whereas heavy thinning reduced growth at the waterlogged TB site. Site-specific differences in tree growth responses to negative pointer years were evaluated, particularly concerning drought events. Resilience metrics computed for five drought pointer years (1976, 2000, 2003, 2015, 2019) showed no consistent differences between thinning intensities; however, at ZR, heavy thinning yielded higher resilience (Rs) and resistance (Rt) in 2015 and 2019. During drought years, the average relative growth reduction (ARGR) ranged from 3% to 31%, with the lowest values under moderate thinning. Overall, moderate thinning enhanced stand productivity and resilience, whereas heavy thinning had adverse effects at the waterlogged site. These results highlight the need to adapt silvicultural practices to local ecological conditions to ensure long-term stability and productivity.

Seasonal and microclimate-responsive expression of VRN-A1 and VRN-B1 in wheat under field conditionsOriginal Paper

Nicole Frantová, Ilja Tom Prá¹il, Ludmila Holková

Czech J. Genet. Plant Breed., 2026, 62(2):53-63 | DOI: 10.17221/76/2025-CJGPB

The need of vernalisation, controlled by the gene VRN-1, impacts wheat adaptation and yield stability, yet field evidence on the plasticity of VRN-1 homoeologs expression is limited. We quantified VRN-1 homoeolog dynamics across two sites and two seasons in seven cultivars, by sampling their apex and leaf. VRN-A1 varied with genotype (P < 0.001***), tissue (apex > leaf; P < 0.001***), apex development (P < 0.001***), day length (P < 0.001***), and to a lesser extent, on short-term freezing exposure, quantified as a 5-day freezing-degree sum (FDS; P = 0.019*). Photoperiod class (Ppd-D1a vs Ppd-D1b) added an additional effect (P = 0.001***). VRN-B1 showed strong genotype effects (P < 0.001***), a modest effect of site on its expression (P = 0.025*), and pronounced associations with microclimate variables (day length, thermal sums, freezing exposure; all P < 0.001***). Directionally, Ppd-D1a backgrounds tended to advance the development while showing earlier apex VRN-A1 peaks. Overall, VRN-A1 expression mainly reflected developmental stage and seasonal forcing, whereas VRN-B1 might be more microclimate-responsive, indicating complementary roles for timing and stress-response plasticity. To isolate causal effects and to further explain these dynamics, targeted sequencing and tests in near-isogenic lines will be needed in future work.

Assessment of genetic diversity and population structure of almond germplasm in Van Province, Türkiye, using iPBS-retrotransposon-based markersOriginal Paper

Berna Sarman, Metin Koçak

[Ahead of Print]CAAS Agricultural Journals, X:X | DOI: 10.17221/12/2026-CJGPB

The genetic characterisation of naturally occurring almond genotypes can guide the selection of genetic resources to be used in the breeding programme. Therefore, this study aims to assess the genetic diversity and population structure of almond germplasm comprising 50 accessions naturally occurring in Van, Türkiye, along with two commercial varieties (Pabuç and Dokuzoğuz). Thirteen inter-primer binding site (iPBS) retrotransposon markers generated a total of 102 bands, of which 95 were polymorphic. The average polymorphic band number per marker was 7.3, with a range of 5 to 13. A formula yielding a maximum of 0.5 resulted in polymorphic information content (PIC) values between 0.27 and 0.43, with a mean value of 0.36. Unweighted pair group method algorithm (UPGMA), principal coordinate analysis (PCoA), and STRUCTURE analysis, based on Bayesian clustering analysis, yielded consistent results, indicating that local populations (Akdamar and Çarpanak) were distinctly grouped, while commercial accessions were clustered with Çarpanak accessions. The diversity metrics and classification analysis utilising 13 iPBS-retrotransposon markers demonstrated that the iPBS-retrotransposon marker system possesses significant promise for evaluating the genetic variety and population structure of almonds.

Advancements in sensor-based weed management: Navigating the future of weed controlReview

Santhappan Vignesh, Palanisamy Murali Arthanari, Rengabashyam Kalpana, Ranganathan Umarani, Subburamu Karthikeyan, Ponnusamy Janaki

Plant Protect. Sci., 2025, 61(2):95-109 | DOI: 10.17221/76/2024-PPS

Controlling weed populations in agricultural land is challenging due to various factors, such as soil conditions, crop type, and environmental conditions. Substantial experience is needed to develop a strategy for minimising pressure from weed infestation. For a relatively longer period, weed control was taken care of using herbicides and mechanical and manual weeding. While herbicides simplify weed control, they pose issues like residual effects and the development of herbicide resistance in weeds, necessitating the deployment of alternate smart weed-management technologies. Lately, smart weeding robots and sensor-based site-specific spraying systems have been developed. Sensors as varied as hyperspectral imaging cameras, Global Navigation Satellite System (GNSS), Real Time Kinematics-Global Positioning System (RTK-GPS), optoelectronic, fluorescence sensors, laser and ultrasonic systems can help to improve weed control efficacy when combined with mechanical and spraying robotic systems. Camera-steered mechanical weeding robots and unmanned aerial vehicles are now widely available for weed management. This review focuses on the developments in sensor-based mechanical and chemical weeding, identification of herbicide-resistant weeds, and herbicide effect assessment. This is a comprehensive overview of studies of sensor-based weed-management strategies being adopted worldwide. Furthermore, an outlook towards future sensor-based weed control strategies and necessary improvements are given.

Greenhouse gas emissions from alluvial soils in grassland and cropland in northern part of Europe’s temperate climate zone (Latvia)Original Paper

Raitis Normunds Meļņiks, Arta Bārdule, Oleh Prysiazhniuk, Oksana Maliarenko, Inga Jansone, Sanita Zute, Aldis Butlers, Andis Lazdiņ¹

Plant Soil Environ., 2026, 72(3):194-209 | DOI: 10.17221/323/2025-PSE

Alluvial soils have high importance for both agriculture and biodiversity; however, these soils can also contribute to greenhouse gas (GHG) emissions including carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4). In this study, we examined GHG fluxes of three grassland and two cropland sites with alluvial soils in Abava river floodplain, Latvia (Europe). Soil CO2 fluxes representing heterotrophic respiration (Rhet) were determined using a portable CO2 gas analyser, while ecosystem respiration (Reco), soil CH4 and N2O fluxes were quantified using a manual closed chamber method combined with gas chromatography. Most alluvial soils acted as source of GHG emissions with the exception of two grassland site where annual CH4 exchange reflected a slight CH4 removal from the atmosphere. Mean total GHG emissions (sum of net CO2, CH4 and N2O) were 7.0 ± 3.3 t CO2 eq./ha/year in grassland sites and 14.5 ± 4.8 t CO2 eq./ha/year in cropland sites. Net CO2 contributed the most to total annual GHG emissions with mean values of 6.2 ± 3.3 t CO2/ha/year in grassland and 13.6 ± 4.8 t CO2/ha/year in cropland sites. Although the number of study sites is limited, the results support that, in the context of climate change mitigation, grassland represents a more climate-friendly type of floodplain land use than cropland in the hemiboreal region.

On-site composting of waste hop biomass: the impact of covering piles on leachate quantity and compost qualityOriginal Paper

Barbara Èeh, Julija Polan¹ek, ®an Tro¹t, Ana Karniènik Klanènik

Plant Soil Environ., 2025, 71(2):109-122 | DOI: 10.17221/197/2024-PSE

Covering hop waste composting piles with semipermeable membrane after the thermophilic phase until spring, when the compost was ready to use, reduced the volume of leachate and leached nutrients amounts significantly; there was a negligible amount of leachate and low amount of leached nutrients during winter and spring at all treatments. At treatments with additives (biochar, preparation effective microorganisms) and larger percent of particles of 2–5 cm, it was indicated that composting pile should probably also be covered in the first months of composting and be opened only when turning/mixing it; on the other hand, the amount of leachate was much lower in a pile without additives and particles of 2–10 cm by the time of covering (2.2- and 2.5-fold less respectively) and the amount of leached nutrients as well. All treatments produced compost without a bad smell, had a total nitrogen content greater than 2%, could be considered as mature (the C : N ratio was below 20) and stable, and the biomass hygienisation threshold was reached. However, there were some significant differences among them. The compost of the pile with added biochar and starting hop waste biomass particles of 2–5 cm was considered phytotoxic (germination index of radish was 31%); also, its other results were less promising in comparison to other treatments. The pile with no additive and the starting particles of waste hop biomass 2 to 10 cm, mixed properly related to regular temperature measurements in the thermophilic phase, reached the best results; the leachate amount and leaked nutrients amount were significantly the lowest, while the final compost contained significantly higher amount of nutrients and had the highest germination index.

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