Fulltext search in archive

Soybean yields can be considerably improved by inoculation with selected Bradyrhizobium japonicum strains and foliar fertilization. An exact field experiment was carried out in 2012-2014 at the Experimental Station of Cultivar Assessment in Przecław, Poland. The test plant was soybean cv. Aldana. The experimental factors were: bacterial inoculant Nitragina (Bradyrhizobium japonicum); foliar fertilization with Mikrokomplex; combined applications Nitragina + Mikrokomplex and the control treatment. Significant effect of Nitragina on an increase in the number of plants prior to harvest, plant height and the number of pods per plant was indicated. Fertilization with Mikrokomplex caused an increase in the number of pods per plant and thousand seed weight. Nitragina + Mikrokomplex increased the number of plants prior to harvest, plant height, the number of pods per plant and thousand seed weight. Seed yield was significantly higher in all the treatments compared to the control (2.31 t/ha). Higher soil plant analysis development values were found after the application of Nitragina + Mikrokomplex, and in the stage of pod development, also after foliar fertilization with Mikrokomplex. Application of Nitragina and Nitragina + Mikrokomplex resulted in an increase in leaf area index and mean tip angle and total protein in seeds. Fe content in seeds was the lowest in the control (69.2 mg/kg) and significantly higher in the other treatments (Nitragina, Nitragina + Mikrokomplex), and Mg content significantly increased after the application of Mikrokomplex and Nitragina + Mikrokomplex.

Forests in the mountain ranges are supposed to meet multiple objectives at the same time. There are different expectations and priorities among stakeholder groups. The object of the research is the case study area in the Goat Backs Mountains in Slovakia (representing Western Carpathians). The stakeholders' panel which included local government officials, landowners, foresters, interest group representatives etc. judged the current and future importance of ecosystem services in a special questionnaire. The context analysis provided main strengths and weaknesses, threats and opportunities that influence mountain forest management and the provision and use of ecosystem services in the case study area were done by involved experts from practice and science. Based on the SWOT results a possible strategy for the provision of ecosystem services was proposed. Some of the key issues that have been identified by experts concerning the balancing of ecosystem service provision are: strengthening the local stakeholder involvement; enhancing regional initiatives; and economic market-oriented instruments and economic incentives.

Alfalfa (Medicago sativa L.) is concluded to be the principal reservoir of Pea streak virus (PeSV, genus Carlavirus) which induces necrotic streaking symptoms in pea. This virus is prevalent in pea growing areas in the USA, but in Europe it was recorded only once almost 60 years ago. Recently, filamentous virus particles 600-700 nm long have been observed in examined plant sap of alfalfa with leaf malformation, local necrotic lesions and yellow spots on leaves. Four kilo base pairs nucleotide sequence of PeSV including partial replicase gene, triple gene block, and capsid protein (CP) gene has been determined. On the nucleotide level, the sequence of the CP has about 80% identity with the North American isolates of PeSV, however, on the amino acid level the sequence has more than 94% identity. This is the first sequence-based proof of PeSV presence in Europe.

This research was conducted to assess the influence of long-term tillage system on soil organic carbon, microbial biomass carbon, root biomass and crop yield in spring wheat-field pea rotation fields in a rainfed semi-arid environment from 2013 through 2015. The treatments were; conventional tillage with stubble removed (T); no-till with stubble removed (NT); no-till with stubble retained (NTS) and conventional tillage with stubble incorporated (TS) arranged in a randomised complete block design with three replicates. The soil organic carbon in NTS increased by 16% and 14% over T and NT. Compared with the T and NT, NTS increased soil microbial biomass carbon by 42% and 38% in 0-30 cm depth, respectively. Root biomass was significantly increased in NTS by 47% and 54% over T and NT, respectively. Across the three years, NTS had an average grain yield of 53% and 41% higher than T and NT, respectively. Compared with NTS, T and NT decreased root biomass by 54% and 48%, respectively. In view of the limited and erratic biomass production in this region, integration of no-till with straw mulching is recommended for soil fertility improvement, environmental quality and sustainable crop production.

A one-year-old, 2.6 kg, spayed female Maltese dog was referred with passage of faeces coming from the vulva, repeated vaginal discharge and vaginitis. Through physical examination, contrast radiograph and vaginoscopic exploration, the dog was diagnosed with rectovaginal fistula which was surgically corrected. After midline perineal incision, the rectovaginal fistula was isolated and transected. The vaginal and rectal defects were closed separately, but dehiscence of the surgical region took place three days after surgery. For the second operation, the rectal segment containing the fistula was removed by transanal rectal pull-through, and anastomosis was performed. Total follow-up time was 10 months and no sign of recurrence was reported. Early postoperative complications included perineal soiling and wound dehiscence, but the long-term outcome was good. This case is the first report of surgical correction of a rectovaginal fistula in a dog with a normal anus.

Increased detection records of Listeria monocytogenes in foods have been recently observed. Standard methods of L. monocytogenes elimination from food products including pasteurisation or acidification may, however, be unreliable. The elimination of L. monocytogenes using specific bacteriophages which are natural antagonists of these pathogenic bacteria has become an innovative method that does not affect the taste, smell or appearance of the product. The use of phage preparations in food production is becoming an increasingly popular method for the effective and safe elimination of L. monocytogenes from food products.

In order to improve the gut microbiome of calves, probiotic bacteria can be fed as active living-cells (fermented milk), or as live but inactive (freeze-dried) cultures. Ten bifidobacterial strains with suitable probiotic properties (as determined in our previous study) were tested for survival during the freeze-drying process, and screened for their ability to ferment cow's milk. The viability of both freeze-dried and live-cell cultures during storage was also tested. All of the strains tested were able to ferment cow's milk, with average counts of 8.26 ± 0.62 log CFU/ml. Eight out of the ten strains were able to survive in milk for 2 months in counts higher than 10⁶ CFU/ml. Bifidobacteria showed high viability following the freeze-drying process, with average numbers of 9.03 ± 0.22 log CFU/vial and did not decrease after 12 months of storage. The mixture of rifampicin-resistant variants of bifidobacteria (RRBs) was fed to 2-day-old dairy Charolais calves in the form of living-cells, or as freeze-dried bacteria. The control group was given no probiotics. Survival of the RRBs administered and the numbers of other bacterial groups in faecal samples was monitored by culturing. Bifidobacteria that were administered passed successfully through the upper parts of the gastrointestinal tract, and were found in numbers higher than 10⁹ CFU/g for two weeks. RRBs colonized the intestines of calves for at least 63 days in both treatment groups. Significantly higher total counts of bifidobacteria were found in the treated groups, compared to the control group. Reduction in Escherichia coli and total coliforms numbers, and an increase in lactobacilli counts were observed in both experimental groups following the application of the probiotic mixtures. Our results show that both forms of administering probiotic bifidobacteria to calves are effective, but that the freeze-dried form is more suitable from a practical viewpoint.

The aim of this work is to determine the compatibility, the scion biomass, and the yield of the grapevine variety Welschriesling grafted onto 12 grapevine rootstocks. As an index of compatibility, the callus development and graft success were determined. Dry weight of canes was measured at the end of the growing seasons (2011-2014), while root dry weight only in the first year in the nursery. The grape yield was measured in the first production year. Welschriesling showed good compatibility with all examined rootstocks. More than 85% of grafts had a complete callus development (8BČ rootstock 100%). The average of graft success in the nursery was 67%, but the average of 5BB, G251, and G103 was above 80%. The G103 rootstock had the highest root dry weight after one season. The dry weight of canes in vineyards was above the average with 5BB, SO4, Binova, Börner, and M V rootstocks. All Georgikon rootstocks had a lower cane dry weight per vine than the others. The highest yields were recorded on SO4, G251, and Börner rootstocks.

The aims of the paper were as follows: (1) to assess the applicability of speckled alder for pioneer stands on the mountainous clear-cut tracts and (2) to evaluate the effects of slow-release fertilizer on the survival and growth of speckled alder under harsh environmental conditions. On the basis of seven-year results we can presume that speckled alder is a convenient pioneer species that can be cultivated on harsh mountain sites where the climatic extremes need to be alleviated and a layer of surface humus renewed. The initial slow-release fertilization is, nonetheless, highly desirable. As contrasted to the control the surface and planting hole applications of amendment reduced the total seven-year mortality rate by 9.5% and 20.1%, respectively, and the periodic annual height increment (2003-2009) was promoted by 47% and 59%, respectively. Analogous results were obtained when the values of basal stem diameter and crown diameter were compared. As for the method of application, the placement of the slow-release amendment in the planting holes seems to be more efficient mainly in terms of survival promoting. The surface application is, nonetheless, less laborious and also yielded satisfactory results.

The aim of this study was to investigate the wood density of Turkish hazel (Corylus colurna L.), an introduced species in the Czech Republic. Sample trees coming from a Czech site were tested for basic wood density and oven-dry density according to Czech national standards. Four sections were taken from each tree to evaluate the variability of wood density along the stem height and along the stem radius. The mean values for the basic density and the oven-dry density were 544 kg.m^-3 and 627 kg.m^-3, respectively. The results show that the highest density can be found in the bottom part of the tree, followed by a decline with the increasing height of the stem. For the radial direction from the pith to the bark, the pattern of density variability was more complex, reaching its highest value close to the cambium. Practically no correlation between annual ring width and density values was found out.

This article summarizes basic estimates of productivity and trend analysis of one of the principal introduced forest tree species in the Czech Republic, i.e. Douglas-fir (Pseudotsuga menziesii [Mirbel] Franco). As a comparison, we also examine grand fir (Abies grandis [D. Don] Lindl), northern red oak (Quercus rubra L. syn. Quercus borealis Michx.) and black locust (Robinia pseudoacacia L). This paper presents estimates of forest land area, standing volume, annual and total increments, distribution of age classes, average ages and site indexes for the period 1979-2010. All data were obtained from the national forest inventory of the Czech Republic. Korf's growth function was used for the assessment of current and mean annual increments (CAI, MAI) of Douglas-fir compared to other tree species. Our results suggest a decline in the annual area afforested by Douglas-fir, as influenced by the State administration management choices, a low rate of an increase in the forest land area, increasing average age of the forests. On the other hand, we observed a dramatic increase in the standing volume as well as high annual increments in volume. Douglas-fir is the most productive major tree species in the Czech Republic and there is a great potential to expand its use throughout the country.

The onset and course of selected vegetative phenological phases of beech along the altitudinal gradient in Slovak Republic were studied. Observations were done in the Burda Mts. (200-300 m a.s.l.), Kremnické vrchy Mts. (500 m a.s.l.) and in the Poľana Mts. (900-1,000 m a.s.l., 1,200-1,400 m a.s.l.). Selected spring phenological phases (budburst and leaf unfolding) as well as autumn phenological phases (autumn colouring and leaf fall) were investigated over the period of 5 years (2007-2011). The earliest onset of spring phenological phases during the period of study was found at the lowest-lying sites in the Burda Mts. By contrast, the latest one was observed at the uppermost site in the Poľana Mts. The dynamics of autumn phenological phases had the opposite course compared to spring phenophases. The earliest onset, observed in the uppermost locality in the Poľana Mts., was gradually delayed with decreasing altitude. The phenological gradient, expressing a shift in the onset of spring phenophases along the gradient, reached the mean values of 2.83-3.00 days per 100 m of an increase in altitude. In the case of autumn phenological phases the gradient ranged from -1.00 to -1.78 days per 100 m. On average, the growing season of beech lasted from 128 to 181 days along the altitudinal gradient. Significant correlations (P < 0.001) were calculated between the date of the onset of phenophases and altitude.

The quantification of stems (under- and overbark), foliage and fine roots in 14-year-old stands of European beech and Norway spruce grown on the same site were evaluated. Therefore, 60 trees of each species were sampled, dry masses of stems and foliage were established and expressed by allometric equations with stem diameter as an independent variable. The spruce allocated a much larger portion of biomass into the foliage than beech. The equations on a tree level were constructed also for specific leaf area and one-sided projected leaf area. Moreover, the quantity and morphological characteristics of fine roots in both stands were surveyed through soil coring. While standing stocks of fine roots were similar in the stands of both tree species, significant interspecies differences occurred in morphological properties of roots. Growth efficiency, expressing annual stem production on a variety of foliage and fine root parameters was calculated. The largest differences, specifically fivefold in favour of spruce, were found in growth efficiency based on a number of root tips.

The serious oak decline was reported for the 1979-80 period and 63.0% of adult oaks died in a mixed oak forest in the Síkfőkút site, Hungary. The data were used to obtain (1) quantitative information on diversity indices of shrub layer and shrub canopy, including foliage cover percentage of the shrub layer, mean cover of shrub species before and after the oak decline and (2) structural information on shrub basal area and shrub foliage arrangement. Since 1972 we have determined diversity indices, cover percentage and basal area of shrubby vegetation on the monitoring and plus plots. A negative relation was detected between Shannon-Wiener and Evenness indices of the shrub layer and living oak tree density. A positive relation was confirmed between basal area and mean cover of dominant woody species (Acer campestre, Acer tataricum and Cornus mas). The mean cover of shrub species except of A. campestre increased non-significantly after the oak decline on the 48 m × 48 m plot. The findings of the study indicate that diversity indices of the shrub layer and mean cover of A. campestre can be used as a principal indicator of natural disturbance in the studied mature stand and the species of the shrub layer respond differently to the decreasing stand density.

Visna/Maedi virus (VMV), a small ruminant lentivirus responsible for lymphoproliferative pneumonia, encephalitis, arthritis and/or mastitis in sheep, has been detected in different non-lymphoid organs. However, only a few investigations have been carried out in lymphoid tissues. In this study, some lymphoid tissues and lymph node draining or non-draining VMV target organs from five sheep infected experimentally by the respiratory route three years previously were investigated. Archival samples of spleen, red bone marrow, caudal mediastinal lymph nodes, mammary lymph nodes, popliteal lymph nodes and mesenteric lymph nodes were tested by PCR for the presence of proviral DNA. Popliteal and mesenteric lymph node samples were tested also by immunohistochemical staining of the viral capsid antigen p28. The proviral DNA was detected by PCR in all the lymphoid tissue samples from the infected sheep. The viral antigen was stained in mononuclear cells in popliteal and mesenteric lymph nodes of the infected sheep. Although the lymph nodes draining the classical target organs seem to be more infected than the others, both the viral capsid antigen and the proviral DNA were present also in lymph nodes draining non-target organs, such as the mesenteric lymph nodes. These findings show the presence of VMV in different lymphoid tissues in the late stages of infection and suggest a potential role of these tissues as a site for viral reservoir and replication, even three years after infection.

The aim of the study was to select wheat-Aegilops biuncialis addition lines carrying Aegilops biuncialis chromosomes differing from those which were introgressed into the wheat-Ae. biuncialis addition lines produced earlier in Martonvásár, Hungary. In the course of the experiments new wheat-Ae. biuncialis addition lines carrying chromosomes 2U^b, 6M^b, 6U^b; 5U^b, 3U^b, 7U^b; 5M^b, 6M^b and 7M^b were selected. The 2U^b disomic addition line is relatively stable, as 91% of the progenies contain this chromosome pair. The 6M^b disomic addition line proved to be dwarf and sterile, but it still exists as a monosomic addition line. Progenies analysed from the 6U^b monosomic addition line did not carry the 6U^b chromosome. One plant containing the 5U^b, 3U^b and 7U^b chromosomes and one plant carrying 5M^b, 6M^b and 7M^b chromosomes showed very low fertility. Each of the plants produced a single seed, but seeds of the parent plants are still available. Line No. 49/00 carried a submetacentric Ae. biuncialis chromosome pair and the chromosome number 44 has been constant for several generations. After FISH no hybridisation site was observed on the Ae. biuncialis chromosome pair using the pSc119.2 and Afa family repetitive DNA probes, so it was not possible to identify the Ae. biuncialis chromosome pair. However, the use of wheat SSR markers and the (GAA)_n microsatellite DNA probe allowed it to be characterised more accurately. These new lines facilitate gene transfer from Ae. biuncialis into cultivated wheat and the selection of U and M genome-specific wheat SSR markers.

Single-domain antibody (sdAb) fragments derived from heavy-chain antibodies of camelids and cartilaginous fish represent a new generation of therapeutic agents and immunoreagents. Due to their unique characteristics, such as low molecular weight, high physical-chemical stability, good water solubility, and the ability to bind antigens inaccessible to conventional antibodies, they could potentially act as a substitute for conventional therapeutic drugs in the treatment of serious human diseases, and, moreover, could be broadly used in analyses and diagnostics. In this review article, an analysis of 826 publications oriented to heavy-chain antibodies and their sdAb fragments indexed in the Web of Science^® database since 1993 has been carried out. Attention has predominantly been paid to papers published from 2010 to June 2012. Key publications are presented in tables and are characterised by descriptive words, abstracts and references. The presented publications have been sorted according to seven basic criteria: review articles and monographs, heavy-chain antibodies of camelids and sharks, production of sdAb fragments using recombinant technology, characteristic properties of sdAb fragments, application of sdAb fragments in therapy, application of sdAb fragments in diagnostic and immunoanalytical methods and other prospective uses of sdAb fragments. This review article should highlight the typical properties of heavy-chain antibodies and sdAb fragments which differentiate them from conventional antibodies and other available recombinant fragments, and also emphasize their extremely broad application potential, mainly in human disease therapy. At the same time it allows an easy and rapid orientation in numerous publications written on this subject, and facilitates the search for the required data.

Assemblages of earthworms were evaluated in spruce and beech stands in the Kienhaida Nature Reserve (Krušné hory Mts., Czech Republic) and its immediate surroundings, where site conditions were modified by the soil preparation for forest regeneration. The beech stands of the reserve showed low diversity but higher earthworm abundance than did the spruce stands, which in turn showed the lowest Lumbricidae abundance (18 individuals.m^-2). The highest abundance of earthworms (124 individuals.m^-2) was found in the soil of mounds created 30 years prior to sampling from the organic soil of the A_h horizon. The highest species diversity and low abundance were characteristic of areas between the mounds, the soil surface of which was greatly disturbed after removal of the A_h horizon to create those mounds. The degree to which the reforested clear-cuts created due to air pollution were overgrown with weeds contributed positively to the diversity and abundance of Lumbricidae.

Recently, geophysical methods have been widely used in many fields including pedology. Two of them, ground penetrating radar (GPR) and vertical electrical sounding (VES) were employed at the Dehtáře experimental site with the aim to evaluate their application in the Cambisol and Stagnosol soil types and crystalline bedrock survey in Czech conditions. These measurements were complemented by the classical soil survey using a gouge auger. As a result, interpreted soil and rock environment profiles were obtained, with the identification of boundaries of Bg, C, and R soil horizons and bedrock at various degrees of weathering. The interpretation of measurement records demonstrated suitability of the VES and GPR method application, using GPR for imaging the soil profile and the top of bedrock, while the VES method gave better results in imaging greater depths. The research demonstrated advantages of the geophysical methods such as instancy, continuous imaging, and no disturbance of the subsurface. In spite of needing classical survey data for interpretation of the results obtained by the geophysical methods, their usage can bring better quality to the soil profile imaging.

The study evaluates the mortality, growth and qualitative characteristics of Douglas fir transplants planted out on clear-cut areas with different shelter. We assessed 11 clearings situated in the Forest Site Complex 4K - acidic beech (Fagetum acidophilum). The young trees were assessed 3 years after planting for the following parameters: total shoot length, increment, root collar diameter, number of multiple stems, stem curvature, crown form, length of needles, colour of needles and frost damage. The lowest mortality and the best growth of Douglas fir plants were recorded in small, sheltered gaps. Large, unsheltered gaps showed low survival, slower growth and poor vitality of plants.

The paper presents results of an assessment of needle age classes in Scots pine partial populations in the period of three and five years after outplanting in a lowland site in relation to conditions created by climatic elements. The method is based on Skuhravý's (1987) description when the state of needle age classes of the third whorl (from the apex) is evaluated just once. Five-year summary of meteorological data was assessed according to the criteria of determination of periods of heat waves and dry spells. Differences in numbers of living needle age classes in the groups of Scots pine populations in the period of three and five years after their outplanting in permanent lowland sites proved the highest total number of living needle classes in the group of local lowland populations. On the contrary, the lowest total number of living needle classes was found out in the group of mountain populations. Critical climatic periods are overlaps of heat waves and dry spells that illustrate the complexity of moisture and temperature conditions of lowland sites. Although the Scots pine is a xerophytic species, it responded to the cumulation of adverse factors by accelerated needle senescence. Its physiological response to heat and dry stress consists in needle shedding, i.e. reduction in the assimilating area. As mountain populations are not usually exposed to heat waves, the impact of this phenomenon on them was the highest. On the other hand, the local (lowland) populations carrying information on the patterns of local climate from the reproduction period do not undergo any marked reduction in needle age classes.

The pine stand in the Havraní Skála locality in the Bohemian Switzerland National Park was affected by an extensive mixed-severity wildfire in summer 2006. The fire severity, fire type and fire extent were assessed by various fire severity measures collected in the field (mean bole char height on stems on sample plots, content of oxidizable C and total N at a soil depth of 0-5 cm), as well as by classification and filtering of green canopy cover from time series of aerial photographs acquired before the fire (2005), soon after the fire (2006) and one year after the fire (2007). The specific image analyses made it possible to uncover the spatial and temporal pattern of the stand defoliation. The central part of the site was mainly affected by the crown fire and thus defoliated substantially right during the fire. On the contrary, the peripheral part of the site was mostly affected by low-severity ground fire and therefore defoliated progressively one year later. All the fire severity measures used were well related.

In recent years more attention has been paid to the issue of pain in animals, particularly in association with increasing awareness of animal welfare. It is therefore necessary for veterinarians to be able recognise unambiguously whether an animal suffers from pain. Adult humans suffering from pain can more or less characterise their painful experiences, including the site and intensity of the pain. However, pain in animals is in some aspects more complex and it can be rather difficult to evaluate the seriousness and impact of painful events. Therefore, in animals we have to recognise the signs of pain according to indirect markers which involve behavioural, physiological and finally clinical responses. Moreover, in particular the behavioural changes associated with pain can be along with the general signs also species-specific, and hardly recognisable (and for an inexperienced observer seemingly unimportant) which makes pain assessment even more complicated. Therefore, the current review formulates definitions of pain, its classification and is focused on methods that may facilitate pain recognition in animals, which is crucial for an effective pain assessment and consequent effective pain management. The review combines recent knowledge with well proven facts concerning pain and furthermore also highlights the author's own research on pain assessment.

The paper presents the evaluation of data on precipitation totals and on SO₄^{2 -}concentrations and S-SO₄^{2 -} fluxes in lysimetric waters in a submontane beech stand. The records cover continually a 19-year period. The site is situated in the Kremnické vrchy Mountains, belonging to the West Carpathians Mountains. The material was sampled on two plots. The first had been clear cut immediately before the research started (1989), the second was covered with the original forest stand. In total, the highest SO₄^{2 -} concentrations on the open plot were measured four years after the intervention (1993) at the soil depth of 0.25 m (40.50 mg.l^-1). The lowest values were recorded in 1989 on the control plot at the soil depth of 0.25 m (4.54 mg.l^-1). The sulphate sulphur deposition was the highest in 1995 in the surface humus layer on the open plot (51.8 kg.ha^-1.year^-1). A very significant influence of precipitation totals on sulphur amounts deposited in the particular soil horizons was also confirmed. No interannual differences in SO₄^{2 -} concentration and S-SO₄^{2 -} fluxwere proved.

To keep forest soils fertile, forest practitioners plant mixed stands that are composed of both economically efficient trees such as conifers and soil-improving broadleaves. This is a mandated practice in the Czech Republic. As the new forest grows, it creates a dense canopy. The canopy is a principal source of organic matter to the forest soil. The formation of new forest humus is particularly important in first-generation forests on the former agricultural soil. Former meadow is a suitable site for forest floor and soil investigation since forest-floor humus covering the surface of the soil is a completely new layer. Both pure evergreen conifer and mixed treatments were planted in 2001. The experimental plot was established in order to investigate performance of tree species and restoration of forest-site conditions. We sampled dead-plant material and 0-10 cm topsoil to investigate their properties under the 10-year-old stands. We compared the treatments by descriptive statistics using both univariate and multivariate techniques. Dry mass (medians of weight) varied among the treatments from 11 to 19 Mg.ha^-1. The forest floor nutrient concentrations appeared to be dependent on the presence of admixed deciduous tree species (sycamore maple, small-leaved linden and European larch) as these forest floors (EL1, EL2, NS3, SM) were higher in base cations and phosphorus compared to pure spruce (NS1, NS2) and pure Douglas fir (DF) treatments. The first axis of PCA ordination plot revealed 45% of total variability and showed a clear distinction between evergreen coniferous and mixed species treatments. Young plantations produced forest floors of different quality; however it was not reflected in the topsoil properties.

Influence of the nitrogen injection fertilization 'controlled uptake long term ammonium nutrition' (CULTAN) on winter rape yield and seed composition was studied in 2009 and 2010 at two sites with different soil-climatic conditions in the Czech Republic. Two conventional treatments consisted of fertilization using the system of divided doses applied on soil surface. Two CULTAN treatments used injection fertilization with the whole dose of nitrogen applied once in the vegetation period, in early spring at the BBCH 26 stage (6 side shoots detectable). The trial compared conventional and CULTAN treatments. The overall dose of nitrogen was 200 kg N/ha in each treatment. The seed yield in a two-year average was 4.83 t/ha at conventional and 4.80 t/ha at the CULTAN treatment. This difference was not statistically significant. The higher nitrogen content in seed was recorded mainly at CULTAN treatments at the Hněvčeves site in 2009. An inconclusively higher phosphorus content was recorded in winter rape seed fertilized with the CULTAN method at both sites in 2010. Content of K, Ca, Mg and S did not show statistically significant differences between the two treatments during both experimental years. In oiliness no differences between conventional and CULTAN methods were observed.

This study was aimed to determine the impact of risk element content on the prevalence of the selected microbial groups in industrial soils of one of the steelworks in Poland. The concentration of heavy metals: Cd, Cr, Cu, Hg, Fe, Ni, Pb and Zn along with soil pH and the number of mesophilic bacteria, fungi, actinomycetes and Azotobacter was assessed in 20 soil samples. The limit concentrations of Cd, Pb and Zn were exceeded in five sites. However, even in these strongly contaminates sites, the studied microorganisms were abundant. The correlation between the concentration of heavy metals and microbial numbers was very weak and statistically insignificant. High numbers of fungi were observed in the contaminated sites, which was particularly interesting in one of the sites, strongly contaminated with Cd, Pb and Zn.

Autotransplantation of teeth is the most natural technique to for replacing missing teeth in exposed parts of the dental arch. Reports from human patients indicate great progress toward successful transplantations. However, complications such as inflammation and ankylosis still occur. To understand regenerative processes after autotransplantations, several animal models have been used (monkeys, rodents, rabbits, cats and dogs) and histological/molecular methods have been established. This review aims to summarise knowledge from animal models and discuss their advantages or disadvantages with respect to possible usage in research.

The Alpine ecosystem is very sensitive to climatic changes, which have an influence on glaciers, snow, vegetation and soils. The aim of this review is to illustrate the effects of global change on the Alpine soil ecosystem, which is an optimal marker to record them. The manuscript enhances our understanding of the global change effect on the Alpine environment: on morphology, on ice, on vegetation and points out how the cycles of soil nutrients equilibrium have been changed with a direct effect on soils that support plant species. The changes in cryosphere, glacier reduction and periglacial environment as glaciers retreat, decrease in the snow cover extent and earlier snowmelt, determine an effect on soils (on the structure, organic matter and humus forms, soil processes and soil types) from the top of the Alpine horizon to the bottom. The processes induced by climate change (such as erosion and tree line shifting) have a direct effect on water balance that can be observed on soil profile characters with an effect on upward migration, change in phenology, extensive losses of species. The equilibrium of the biogeochemical cycles has been changed and this has a direct effect on soils that support plant species.

Long-term field experiments are important for assessing the yield response of crops to different tillage systems and pre-crops. An experiment was established in 1996 in Raasdorf (Austria) on a chernozem with four tillage treatments (mouldboard ploughing (MP), no-till (NT), deep conservation tillage and shallow conservation tillage) and two crop rotations. Winter wheat yields were generally at similar levels with all four tillage systems in most years between 1998 and 2012. Yields increased with higher amounts of rainfall during the vegetation period (from October until June) with the smallest increase among tillage treatments in NT. This indicates that MP can be superior to NT regarding yield at higher amounts of rainfall. Pre-crops considerably influenced winter wheat with higher yields after maize, soybean and winter wheat than after sugar beet. In one year with high rainfall, a tillage × pre-crop interaction showed that yields were lower after maize in NT than in other tillage systems whereas yields after sugar beet tended to be higher with NT in years with low rainfall.