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A 4.3 kg, nine-year-old, spayed female Shih Tzu was presented for a two-month history of seizures, ataxia, and hyper-salivation. A diagnosis of a splenophrenic shunt was made by use of computed tomography angiography with volume-rendered imaging. A cellophane band was placed around the shunt after its isolation from the central tendon of the diaphragm. Clinical signs continued to wax and wane. Preprandial and postprandial bile acids levels were still elevated 10 months after surgery. An ameroid ring constrictor was placed around the shunt vessel before the vessel entered the diaphragm from its caudal aspect. At three months after the second surgery, the dog was near the normal ranges of preprandial and postprandial bile acids. Although a study of the anatomy of different types of extrahepatic portosystemic shunts has been reported in dogs, to the authors' knowledge, there is a lack of information on clinical presentation, treatment, and postoperative results in a specific type of extrahepatic portosystemic shunt, such as a splenophrenic shunt. Cellophane banding should be avoided for occlusion of a splenophrenic shunt passing along the central tendon of the diaphragm.

The objective of this study was to analyze the effect of tillage systems and nitrogen fertilization on the grain yield and quality of spring wheat and on selected chemical and biological properties of soil. The first order factor was the tillage system: (1) conventional (CT) - shallow ploughing and harrowing after harvest of the previous crop, and pre-winter ploughing; (2) reduced (RT) - only a cultivator after harvest of the previous crop, and (3) no-tillage (NT) - only Roundup 360 SL herbicide (a.s. glyphosate) after harvest of the previous crop. The second order factor was nitrogen dose: (1) 90 kg N/ha and (2) 150 kg N/ha. Higher yields were demonstrated for spring wheat sown in CT and RT systems, compared to the NT system. In addition, grain yield was increased by a nitrogen dose of 150 kg/ha, compared to 90 kg/ha. Contents of protein and wet gluten in the grain were also increased by the higher nitrogen dose. The RT and NT systems were observed to increase the content of organic C, total N and available phosphorus in the soil, compared to the CT system. They also increased the number and mass of earthworms in the soil, compared to the CT system.

The present study was aimed at the detection and describing the lesions of akabane virus in foetal and new-born calves tissues using immunohistochemical and immunofluorescence techniques. Akabane virus lesions were evaluated in 12 foetuses and three new-born calves using serological and pathological methods and immunohistochemistry and immunofluorescence. Macroscopically, prominent arthrogryposis and hydranencephaly (A-H syndrome) were the main symptoms. At the histopathological examination, lesions were especially localised in the midbrain, pons, and medulla oblongata in the central nervous system (CNS) in calves naturally infected with akabane virus. In these areas, degenerative and necrotic neurons were observed. There was prominent mononuclear infiltration in perivascular areas. While akabane virus antigen was only detected in brain using immunofluorescence, immunohistochemistry against akabane virus yielded positive antigenic reactions in the cerebrum, cerebellum, liver, spleen and kidneys. In addition to these findings, there was a relationship between akabane virus infection and neurofilament (NF), glial fibrillary acidic protein (GFAP), and N-methyl-D-aspartate receptor (NMDAR) immunoreaction in astrocytes and neurons.

Statistical methods of imputation allow predicting genotypes of markers (which were not genotyped in the whole population) based on known linkage disequilibrium relationships between the flanking polymorphisms and the information obtained from reference datasets used as a pattern. In this study we attempted to predict genotypes of two bovine spongiform encephalopathy (BSE) susceptibility associated indel polymorphisms located in the promoter region of PRNP gene relying on the data obtained from middle density SNPs arrays in a sample of the population of Holstein cattle. The two SNPs panels spanning PRNP locus were tested in terms of imputation efficiency. Both panels gave satisfactory imputation results showing high accuracy and high probabilities of imputed genotypes. Our results suggest that the approach applied can be used to evaluate the frequency of the disease associated polymorphisms in large populations of animals genotyped with whole-genome SNPs panels based on a limited-size reference population and small financial outlays.

To avoid ecosystem degradation, forestry planning needs to be based on current information about the state of forest environment. Phytoindication is an inexpensive tool that allows tracking the environmental change at fine spatial scales. The present study uses this approach to assess changes within abiotic conditions of forests in the area of the Moravian-Silesian Beskids Mts. (Czech Republic). Phytosociological relevés collected in 2013 at 118 permanent plots were compared with records from the 1960s and 1970s. The changes were expressed using average Ellenberg's Indicator Values and units of the Czech Forest Typological System. Persisting soil acidification was detected, and linked to industrial deposition and planting of Norway spruce beyond its natural range. Conversion towards a higher share of broadleaved species was suggested to support soil recovery.

The article presents the possibilities of visual and statistical outputs from the telemetric tracking of game: activity data, heat map, home regions, movement routes and the points of occurrence. Nowadays the methods of the telemetric tracking of game are also used for finding the best ways to eliminate damage caused by wild boar generally, and field crops damage specifically. From telemetrically gained data it is possible to study the local habits of wild boar and their preference of crops and cultivars in various periods. On the basis of this knowledge it is possible to implement the necessary agrotechnical measures. The pilot processing and verification is run on the portal Zvěř (game) online (http://zver.agris.cz/). Currently there are 11 wild boars marked and tracked. The public part of portal is used for the basic presentation of data; in the non-public part the data of private subjects (agricultural companies and hunting organizations) that are not interested in public presentation are processed in the same way. In this way there is at disposal an integral system of wild boar tracking: capturing, marking, data collection, processing and presentation. This system can be used by research institutions, farmers and hunters.

The aim of EU legislators with respect to experiments on animals was to reduce to an absolute minimum both the number of animals used in experiments and their suffering. This study presents, from a historical point of view, expectations related to the coming into force on 1 January 2014 of Directive 2010/63/EU adopted on 22 September 2010. At the same time, it points out that, in some situations, carrying out experiments on animals in practice is different than in theory. The study calls attention to the issue of mere literal conformity of the Polish law with EU directives, illustrating it with examples and takes a critical view of the omission of veterinarians (Veterinary Inspection) from the supervision of experiments.

The present study examines the relationships between the indicators of tree species and structural diversity and the quality of timber production in young even-aged forest stands with the average age below 40 years. The study is based on the forest inventory data from University Forest Enterprise Kostelec nad Černými lesy, Czech Republic, performed from 2009 to 2011. The examined young stands were recorded in 256 sample plots representing 21.2% of the enterprise area. On each sample plot, we quantified 171 partial biodiversity indicators. In total, we analysed 16,416 different variants of the relationship between the diversity indicator and the quality of timber production. The analysis revealed that similarity indicators such as the range of tree heights, Canberra distance, Bray and Curtis index, and index of species evenness and heterogeneity were the most frequent basic indicators occurring in significant correlations. The results indicate a positive relationship between the proportions of assortments in quality classes I to IV and stand diversity expressed by the number of tree species and Canberra distance.

Contamination of Pansky Pond, in March 2013, with 119 mg/l aluminium, and 87 mg/l iron by acidic (pH 3.17) inflow from a nearby quarry caused fish die-off, while exhibiting symptoms of suffocation. Transformation of soluble forms of aluminium and iron into insoluble forms occurred on fish gill where the content of aluminium and iron was 100-fold and 12-fold, respectively, that found in control fish in an unaffected pond. In addition to insoluble aluminium and iron, gills showed presence of iron bacteria. Histopathology was characterised by expression of reactive processes and regressive alterations resulting in gill tissue necrosis. Impairment of the excretory function of gills was reflected in significantly (P < 0.01) higher concentrations of ammonia in the blood plasma of exposed fish compared to the control. Damage to parenchymatous tissues (kidney, liver, spleen) of the exposed fish was manifested as dystrophic alterations, higher aluminium and iron content, and enhanced activity of transaminases in blood plasma compared to the control.

The aim of this work is to evaluate the changes of Mehlich 3 - P, K, Ca and Mg contents in soil during a long-term field experiments with sewage sludge, farmyard manure (FYM) and mineral NPK (NPK) application, compared to the control non-fertilized treatment. The experiment was established at the Humpolec and Suchdol sites (Czech Republic). Potatoes, wheat and barley were grown in crop rotation. Fertilizing system was based on the same nitrogen dose of 330 kg N/ha per one crop rotation. Archive soil samples from the beginning of the experiment (1996) and from the end of each year's crop rotation (1999, 2002, 2005, 2008 and 2011) were analyzed. In spite of the different soil-climatic conditions of the studied sites, very similar tendencies of P, K, Ca and Mg contents changes after the fertilizing systems used were observed in the soil. In case of the same nitrogen dose (330 kg N/ha), sewage sludge appeared to be better source of bioavailable soil phosphorus compared to the farmyard manure and NPK. On the contrary, FYM was a better source of bioavailable potassium and magnesium, despite the lower total magnesium content in FYM. The NPK treatment was the best long-term source of bioavailable potassium.

Radiographic contrast studies have been recommended to identify the thoracic duct (TD) and its branches before and after surgery for total occlusion. The macroscopic identification of the TD and its branches during surgery usually involves injection of methylene blue (MB). Radiographic contrast and methylene blue can be injected into different anatomical structures (lymph node, lymph vessel, s.c.). The purpose of this study was to compare two different techniques (radiographic and thoracoscopic) for visualisation of the TD after intrapopliteal lymph node injection in the pig. Six piglets from the same litter (two males and four females), two months of age were used. Iohexol at 245 mg/ml was injected into the left popliteal lymph node (LN) under general anaesthesia; hindlimb, abdominal, and thoracic radiographs were taken. A 0.25% methylene blue solution was injected into the right popliteal lymph node and the thoracic duct colouration was assessed thoracoscopically. The thoracic duct was visualised radiographically in one out of six pigs after iohexol injection and thoracoscopically in five out of six pigs after methylene blue injection. The difference was statistically significant (P = 0.040). Popliteal LN lymphangiography using iohexol at 245 mg/ml in piglets should not be the recommended method for TD visualisation. Intrapopliteal injection of a 0.25% solution of methylene blue is recommended as a method of TD visualisation prior to thoracic duct ligation.

The spread pattern of Abutilon theophrasti (velvetleaf) in Austria, the Czech Republic, and Slovakia was analysed based on an exhaustive distribution data set (389 records). Cumulative number of all records showed a continuous increase since its introduction in the 19^th century and > 1970 its spread gained momentum with an exponential increase > 2000. In fields, the species remained rare until 2000. Since then, A. theophrasti has invaded fields much more frequently (78% of all records in fields > 2000) and it was mainly found in sugar beet and maize. Approximately a total of 188 000 ha of the agricultural area are currently at risk of being invaded. Given the on-going spread into fields, the results provide evidence that A. theophrasti may cause serious impacts. Control measures should focus on the prevention of spreading seeds and the establishment of new foci.

To investigate the effect of fertilization practice on dissolved organic nitrogen (DON) in aggregates, the study was conducted and involved four fertilization treatments: no fertilizer; mineral fertilizer (MF); pig manure matched with mineral fertilizer (MFP) and crop straw matched with mineral fertilizer (MFR). The results showed that DON content and storage were the highest in > 5 mm aggregates and were the lowest in < 0.25 mm aggregates. Compared with MF, MFP and MFR significantly increased DON contents in > 5 mm by 404.7% and 184.4%. In comparison with MFR, DON content and storage in > 5 mm aggregates for MFP were significantly enhanced by 77.5% and 75.0%. A significantly positive linear correlation relationship between DON content and microbial biomass carbon content was observed in aggregates (R² = 0.84; P < 0.01). The results suggest that pig manure matched with mineral fertilizer is a preferred strategy for retaining DON nutrient due to enhanced microbial biomass in aggregates on sloping upland of purple soil.

The aim of this work was to compare herbicide efficacy and reduction of weed reproduction after the application of three frequently used soil residual herbicides during pre-emergent (PRE) and early post-emergent (EPOST) application in maize. Plot field trials were carried out in Central Bohemia during two growing seasons (2010 and 2011). Good efficacy (88%, resp. 83%) was found in isoxaflutole + thiencarbazone (ISF + THC) and terbuthylazin + S-metolachlor (TBA + SMC) on Echinochloa crus-galli, especially in PRE application use. Efficacy on Amaranthus retroflexus was 91% at both tested application periods and there were no significant differences between experimental years. Significant differences in A. retroflexus control were recorded among all tested herbicides (ISF + THC > TBA + SMC > pendimethalin (PEM) + dimethenamid (DMA)). No significant differences between PRE and EPOST were recorded in efficacy on Chenopodium album. Significant differences in efficacy on C. album were recorded among all tested herbicides (ISF + THC > TBA + SMC > PEM + DMA). Mercurialis annua was the most tolerant tested weed, which was significantly better controlled at EPOST herbicide application (73%) compared to PRE application (32%). TBA + SMC showed a significantly higher efficacy on M. annua compared with other tested herbicides. Seed production of all tested weeds was strongly effected by weather conditions, which were significant during 2011, when there was higher than average precipitation during May and June. The most seeds were produced by A. retroflexus > C. album > E. crus-galli > M. annua. ISF + THC best reduced seed production of E. crus-galli, A. retroflexus and C. album, especially when applied at PRE. TBA + SMC best reduced seed production of M. annua. Weed competition on untreated control plots caused yield loss by 90%and 47% in 2011 and 2012, respectively, compared to treatments with the highest yield (ISF + THC).

The aim of this study is to identify a period of time over which smaller, less costly tree transplants can reach the height of larger tree transplants and thus offset their performance. The following Central European native tree species were used: Quercus robur L., Carpinus betulus L., Fraxinus excelsior L., Acer campestre L., Acer pseudoplatanus L., Tilia cordata Mill. and Prunus avium L. In the period after planting, the best growth was observed for small tree transplants. These transplants reached the height of the medium-sized tree transplants for all species except C. betulus. However, the large transplants of A. campestre, F. excelsior and P. avium were still significantly taller than the medium-sized transplants ten years after planting. In contrast, slow growth was observed for the large tree transplants of C. betulus and Q. robur. During the monitoring period, the height of the medium-sized transplants of C. betulus even exceeded the height of the large transplants of this species. These differences suggest that the differences in the establishment rates of individual species are reflected in the growth rates of their plantations during longer periods after planting.

The objective of the present study was to evaluate the effect of P, Ca, S, and/or K and/or Mg application at different rates on the botanical composition, herbage yield and its nutritive value in dehesas developed on calcareous soils. In two growing seasons, 2009/10 and 2010/11, five surface-broadcast fertiliser treatments were applied once in autumn. Two grazing periods were simulated by cutting the pasture in mid-spring and late-spring. Treatment K1, with the highest amount of Ca and without K in its composition, provided higher crude protein values in the herbage than controls in 2009/10. When the effect of each nutrient was analysed separately, a strong and highly positive response of Mg application on herbage production was recorded. A surface application of fertilisers containing 25 kg Mg/ha in autumn could increase herbage yield by 40% under the studied conditions. However, Mg was not applied alone, and thus this increase could be due to an effect of Mg by itself or to a synergistic effect between Mg and other nutrients applied. The application of Ca, P and Mg may also favour the production of high-quality forage by yielding a greater cover of legumes and digestible protein.

This article describes two cases of augmentation of ruptured tendon with fresh frozen Achilles tendon allograft (FFATA) in dogs. Case 1 was a two-year-old crossbreed dog (29 kg) that presented with an open wound on the right forelimb and with complete rupture of the flexor carpi ulnaris and superficial digital flexor tendons. Case 2 was a four-year-old crossbreed dog (4 kg) with partial ruptures of the patellar tendon and detachment of the tibial tuberosity in the right hind limb. In both cases, the ends of the ruptured tendon were sutured and apposed after debridement. To minimize suture failure, FFATA (cut to sufficient size) was placed across the primary suture with tension and sutured to the host tendon. In addition, Case 2 received a Krackow suture through a transverse bone tunnel made in the tibia to fix the patellar tendon along with the tibial tuberosity in situ. The surgical areas healed without any evidence of exaggerated inflammatory response or clinical signs consistent with rejection of the allograft. Both the dogs had normal ambulation and weight bearing on the affected limb 12 weeks postoperatively. No postoperative complications were observed during a one-year follow up period except for slight contracture of the carpus and digits of the affected limb in Case 1. Thus, ruptured tendons can be successfully repaired using suture and augmentation with FFCTA. Augmentation with FFATA may provide additional stability, which counters tension on the primary repair and reduces the chance of gap formation or suture failure in case of reconstruction of the damaged tendon in dogs.

Here, we describe a case of a wild female badger (a sow) with disseminated serous adenocarcinoma of the ovary which corresponds to a group of low grade serous carcinomas of the ovary in humans. Beside grossly apparent dissemination of the disease we observed a scale of histological features classifiable as a precursor lesion - borderline serous tumour of the ovary with implant metastases at the peritoneum, and features of the borderline tumour transformation in the carcinoma. The latter features included invasion of some of the metastatic peritoneal implants into the adipose tissue of the mesentery, retroperitoneum, and in the muscle of diaphragm with lymphangioinvasion and with blood-borne metastatic disease in the lungs. The primary tumour and its metastases had a uniform cytological appearance without atypia of the tumour cells. Mitotic activity was exceptional. The proliferation activity as demonstrated by immunohistochemical investigation of Ki-67 protein expression (revealing all active phases of the cell cycle - G1, S, G2, M) showed a low proliferation activity of the tumour cells, comparable with findings in low grade carcinomas or borderline tumours of the ovaries in women. WT1 protein was expressed in the whole tumour cell population. All these features were diagnostic of serous carcinoma of the ovary with low grade malignant potential. Tumours of the ovaries in wildlife have been described previously but they are infrequent and are rarely classified histopathologically. This case report offers a parallel with serous carcinomas in human pathology including features of transformation from a precursor lesion of a borderline serous tumour into a serous low grade carcinoma.

The aetiology of Crohn's disease and paratuberculosis are the subjects of intensive study and also frequently, of dispute. However, a number of other nosological entities have a similar history, namely type 1 diabetes, multiple sclerosis, sarcoidosis, asthma, psoriasis, spondylarthritis, Blau syndrom etc. The zoonotic risk of Mycobacterium avium subsp. paratuberculosis (MAP) has been discussed for more than one hundred years. "The problem remains open, further research is needed", is the sentence which seems to be obligatory in the conclusions of many scientific articles. A number of hypotheses have been suggested, all with a grain of truth in them. The infection hypothesis has many supporters and opponents, but it does not fit to all Crohn's disease cases. The contribution of the genetic factor has been admitted a long time ago and has been experimentally confirmed by recent excellent studies. An environmental factor is expected and has been often mentioned, but has yet to be discovered. Muramyl dipeptide, derived from peptidoglycans of the bacterial cell wall is one of the triggers, mentioned in connection with chronic inflammatory diseases. The immunomodulatory ability of this compound has been recognised for decades and is exploited in Freund's adjuvant. A critical amount of muramyl dipeptide can affect immunity during some bacterial infections but the long latent period between infection and onset of the clinical form of the disease could explain why a causative relationship between the primary infection and chronic inflammation is not considered. Different species of mycobacteria can be found in the environment, in water, dust, soil and aerosol. Although severe infections with mycobacteria have been described, these species are not thought to be typical zoonotic pathogens. Muramyl dipeptide derived from mycobacteria obviously plays a starring role as a bacterial trigger in the aetiology of many autoimmune and autoinflammatory diseases. Paratuberculosis in cattle and other ruminants is a source of enormous contamination of the environment but also of milk and meat by MAP. Muramyl dipeptide from mycobacteria, namely MAP, and Crohn's disease as a representative of diseases often called civilization threats, are important pieces of the gigantic puzzle. Mycobacteria in the environment and foodstuffs have to be acknowledged as a public health risk, which can never be completely eliminated. There is no reason to push the panic button, but we must learn how to live together with this microorganism, how the pool of immunomodulator sources can be diminished, and how the pathogenic relationship between triggers and target tissues can be disrupted. The dissemination of knowledge, the availability of rapid and inexpensive tools for identification of mycobacteria in different matrices, and the establishment of a maximal allowed limit for mycobacteria in milk and meat should contribute to food safety and consumer protection.

A study was conducted to investigate the influence of sodicity induced changes in soil physical properties on paddy root growth in the normal agriculture, semi-reclaimed and sodic soils. The root growth (length, length density, biomass and distribution pattern) were unfavourably affected by the soil physical properties (bulk density, soil aggregate stability, available water content, hydraulic conductivity and soil water retention potential) in the case of sodic soil. The microbial biomass carbon, bacterial, fungal population and dehydrogenase activity showed the lower values in the case of sodosol compared to the normal soil. These soil biological properties tend to sustain paddy root growth in normal and semi-reclaimed soils. Principal component analysis revealed that soil physical properties accounted for 98.2% of total variance in root growth. The study revealed that salt stress induces changes in soil physical properties limiting paddy root growth in the salt affected soils. It is important to reclaim sodosols to alleviate salt induced physical stress for optimum paddy root growth.

An eleven-year-old, 3-kg, intact female Maltese was presented for depression, anorexia and dyspnoea. Thoracic radiographs showed right-side pleural effusion, a vesicular emphysema pattern and consolidation of the right middle lung lobe. A presumptive diagnosis of lung lobe torsion (LLT) in the right middle lobe was made using a computed tomography scan. Thoracoscopic exploration was performed without one-lung ventilation in the right hemithorax. Pleural fluid and engorgement of the torsed lung lobe were observed. A total lung lobectomy of the right middle lung lobe was performed with a thoracoscopic-assisted procedure using hem-o-lok clips applied to the hilus. The dog was discharged three days after surgery, and no recurrence of the clinical signs of disease related to the respiratory system was observed during the follow-up time period of six months. Based on these findings, we conclude that thoracoscopic-assisted lung lobectomy using hem-o-lok clips is a minimally invasive surgical alternative to thoracotomy and successful operative intervention for lung lesions in small breed dogs.

Despite some progresses in the political situation, the effects of the economic crisis and widespread food insecurity are expected to persist. Humanitarian assistance is now needed to support the needs of the most vulnerable. In the longer term, countries have the significant goal of consolidating peace and security and strengthening the overall governance, while at the same time, reconstructing and rehabilitating their economy.

Potential application of a metal oxide semiconductor based electronic nose (e-nose) as a non-destructive instrument for monitoring the change in volatile production of banana during the ripening process was studied. The proposed e-nose does not need any advanced or expensive laboratory equipment and proved to be reliable in recording meaningful differences between ripening stages. Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), Soft Independent Modelling of Class Analogy (SIMCA) and Support Vector Machines (SVM) techniques were used for this purpose. Results showed that the proposed e-nose can distinguish between different ripening stages. The e-nose was able to detect a clear difference in the aroma fingerprint of banana when using SVM analysis compared with PCA and LDA, SIMCA analysis. Using SVM analysis, it was possible to differentiate and to classify the different banana ripening stages, and this method was able to classify 98.66% of the total samples in each respective group. Sensor array capabilities in the classification of ripening stages using loading analysis and SVM and SIMCA were also investigated, which leads to develop the application of a specific e-nose system by applying the most effective sensors or ignoring the redundant sensors.

Wild boar is a native species in the fauna of the Czech Republic. It is becoming a problematic game species both in the Czech Republic and in other European countries due to its harmful impacts. So far no suitable method of its sustainable management has been devised. Yet, a correct abundance estimate is essential for proper management, field crop damage prevention and proper hunting planning. This paper employed faecal pellet group count to estimate the wild boar density in a forest environment. The research was conducted in a study area covering 12.83 km², which is surrounded by farmland. The count was done under ideal conditions in early spring, after snow had melted. The study area was organised into several differing environments in which different dung densities were expected. The presence of feeding sites, where wild boars concentrated, was taken into account. The conducted count confirmed differences in the utilization of different habitats within the study area. The resulting wild boar abundance was estimated at 64.3 (± 8.9; 95% CI) indd.km^-2.

Communities of xylotrophic fungi were studied in wood of Pinus mugo of different qualities: (i) living stems, (ii) cut stumps, (iii) burned snags, (iv) cut burned stumps, (v) stems recently killed by root rot, and (vi) old snags of root rot-killed trees. A total of 277 isolates representing 58 fungal taxa were obtained from 300 wood samples (50 samples per each substrate category). Results of the present study suggested that following different disturbances (tree felling, forest fire or root rot), fungal communities likely evolve in different directions: depending on its origin (cut, burned or killed by the disease), dead wood might be inhabited by principally different microbial assemblages, and that fire has less effect on community structures than tree felling or root rot.

A long-term experiment was used to evaluate the effects of different nutrient management practices on the distribution of soil organic N fractions and their contribution to N nutrition of a rice-wheat system. Continuous rice-wheat cultivation for 13 years without any fertilization was unable to maintain total soil nitrogen level to its original level and resulted in a decrease at 8.3 mg N/kg/year. Likewise, amino acid N, amino sugar N, ammonia N, hydrolysable unknown N, total hydrolysable N and non-hydrolysable N decreased by 37.2, 29.6, 33.7, 10.4, 26.6 and 20.4%, respectively over their initial status. However, application of inorganic fertilizers alone or in combination with organic manures led to a marked increase in total N and its fractions. The increase in total N with the application of farmyard manure, press mud and green manure along with inorganic fertilizer over treatment with inorganic fertilizer alone was 23.1, 34.4 and 7.0%, respectively. These results imply that integrated use of inorganic fertilizers with organic manures represent a sound practice for sustaining N reserves in soil. On average, amino acid-N, amino sugar-N, ammonia-N and hydrolysable unknown-N constituted about 27.9, 10.7, 28.7 and 32.7% of the total hydrolysable-N, respectively.

Equine grass sickness (EGS) is a degenerative polyneuropathy affecting postganglionic parasympathetic and sympathetic neurons. The major clinical signs relate to dysfunction of the gastrointestinal tract and the condition is frequently fatal. EGS has been reported in different parts of the world including Europe. This paper describes the first case of equine dysautonomia in the Czech Republic. The ante mortem diagnosis was based on typical clinical signs and a positive phenylephrine eye-drop test and was confirmed at necropsy following observation of pathognomic histopathological lesions in the enteric neural system of the ileum.

Public-Private Partnerships (PPPs) are based on co-operation between the public and private sector. The reason for using it is a lack of public financial sources. For this reason, in most PPPs the management and financing of the project is entrusted to the private sector. In the Czech Republic, the widest development area for PPPs in the scope of the Ministry of Agriculture is probably water supply. The further areas for using of PPPs in the scope of this Ministry are the following: forestry, flood protection, adjustment of water flow, security of water sources, builging of the strategic foodstuff store. Important attempts have been made within the last year to increase the implementation of PPPs in water supply. These attempts are based on operation models similar to the BOT (Build Operate Transfer) and the DBFO (Design Build Finance Operate). In addition, the Czech Parliament adopted a law No. 139/2006 Coll., on concessions procedure and concession treaty which entered into force in July, 1^st, 2006 and which brings the legal framework for realisation of the PPPs. There are some legal barriers which limited wide using of the PPPs. It is in the case of public-private venture companies (the limitation is in public procurement law). Public-private venture companies - which refer to the situation where both the private and the public sector holds equity, and, consequently, the company is controlled by the private as well as the public sector - should be the ideal form of PPPs in the areas which are in the scope of the Ministry of Agriculture.

We measured the long-term (28 years) sustainability of rice-wheat cropping system under integrated nutrient management practices emphasizing the trends in grain yields, sustainable yield index (SYI) and nutrient budgeting. The data of long-term experiment revealed that grain yield of both rice and wheat declined under control and sub-optimal fertilizer inputs (50% or 75% recommended fertilizer NPK). Negative yield trend (slope) was observed in control plots for rice (-0.0296) and wheat (-0.0070); whereas positive yield trend was observed under treatments receiving organic supplements. The SYI values indicate that rice yields are more sustainable than wheat. Data on apparent nutrient balance showed a deficit of N (-42.2 kg/ha/year), P (-9.1 kg/ha/year) and K (-52.2 kg/ha/year) under control plots. Surprisingly, there was net depletion of K under the organic supplemented plots. Correlation study revealed that apparent balance of K was negatively correlated with SYI (r = -0.921 for rice; r = -0.914 for wheat) and yield slope (r = -0.870 for rice; r = -0.896 for wheat). If the trend of K imbalance is not reversed, the potential to improve N and P fertilizer use efficiency and crop yields will be limited.

A 13-year-old spayed bitch was referred for evaluation of an abdominal distension with a palpable, continuously growing mass. Abdominal ultrasonography revealed a 30 × 20 cm mass directly connected to the spleen. Surgical exploration confirmed the sonographic diagnosis with adhesions to the omentum and the liver. Pathohistological samples revealed well differentiated adipose tissue and variably differentiated collagenous and myxomatous tissue. Immunohistochemically, vimentin and in some regions alpha smooth muscle actin were expressed indicating smooth muscle differentiation. The results support the diagnosis of a malignant mesenchymoma composed of liposarcoma, mixosarcoma and leiomyosarcoma. No local recurrence or metastasis occurred during a nine month follow-up. So far, only two pathological retrospective studies describing the common prevalence and properties of canine splenic malignant mesenchymomas were found in the literature. However, this rare tumor entity has to be considered as a differential diagnosis in cases of large splenic masses.