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The paper deals with the establishment of the coppice and coppice-with-standards research object under the project Biodiversity and Target Management of Endangered and Protected Species in Coppices and Coppices-with-Standards Included in the System of NATURA 2000. It summarizes reasoning which preceded the selection of the site and provides a detailed description of the methodology of experimental site establishment. It specifies the rules of felling which was planned with the objective to simulate the impact of coppice and coppice-with-standards on biodiversity of endangered and protected species. It also describes the stand condition prior to and after the implemented felling, with additional emphasis on coppice-with-standards. Individual felling variants which were implemented were characterized by varying felling intensity. Close attention is paid to the evaluation of standards which is expressed by a system of score classes.

An experiment with seedlings of Alnus viridis was established on a clear-felled tract situated on a summit tableland of the Jizerské hory Mts. (central Europe, Czech Republic) at an altitude of 980 m a.s.l. The aims of the experiment were (i) to evaluate the response of A. viridis to the application of a basic mixture containing crushed limestone and amphibolite and (ii) to assess the growth potential of the species in a poor acidic site under climatically harsh conditions. Alnus viridis showed a good growth performance and survival rate, its initial response to fertilization was positive. The height growth and expansion of crowns were slightly but significantly stimulated by the treatment, the survival rate was also increased, though not significantly. The amendment application temporarily increased the foliar Ca and Mg concentrations. The content of foliar P, of the most deficient macroelement, was not improved by the treatment. However, the positive effect of fertilization was short-lasting and unconvincing from the aspect of practical use. In the second half of the reference period the control started to gain on the fertilized variant and as for mean height and crown diameter, both variants got almost equalized eight growing seasons after the treatment.

During the archaeological excavation of a multicultural settlement at the Nitra-Chrenova site (south-western Slovakia) an assemblage of animal bone remains was revealed. In one of the settlement features dated to the Roman period a complete horse skeleton was discovered. An investigation was carried out in the hind limb of the horse's skeleton involving macroscopic and radiographic analysis. Exostoses were observed on the tibia, talus, calcaneus, tarsal and metatarsal bones. The articular surfaces were destroyed. The anatomical structure of the talus, calcaneus and tarsal bones was not visible due to new bone formation. Additionally, osteomyelitis was observed in the talus, calcaneus and tarsal bones. It is suggested that the pathological changes developed during the septic inflammation process as a consequence of the complicated wound of the tarsal region or the tarsal joint perforating trauma.

Introgression lines derived from Oryza minuta and O. sativa subsp. japonica var. Junambyeo were crossed for a mapping of the population composed of 112 recombinant lines to identify putative QTLs against rice blast disease using the percentage of diseased leaf area. By using 148 Sequence Tagged Site (STS) and Single Sequence Repeat (SSR) markers, five QTLs on chromosomes 6, 7, 9 and 11 and seven epistatic QTLs were identified against two blast isolates (KI307 and KI209). Of them two QTLs (qKI307-2 and qKI209-3) shared a similar position on chromosome 11. O. minuta introgression contributed the resistance allele for all of these QTLs. Combined phenotypic variations by QTL and (E-QTL) accounted for 56.9% against KI307, and 53.4% against KI209. Each QTL could account for the resistance variation between 11 and 24.6%. The resistance from wild introgressions was attributable to a combination of QTLs and epistatic effects between different loci, capable of inducing hypersensitive reactions. Our findings are in support of the strategy of pyramiding major QTLs to develop improved rice varieties with durable broad spectrum resistance against the blast fungus.

In 2004-2009, a small-plot trial was conducted on permanent grassland dominated by Festuca arundinacea Schreb. on the mesohygrophytic site in order to evaluate effects of four levels of nutrition: F₁ = without NPK fertilization, F₂ = P₃₀ + K₆₀, F₃ = N90 + P₃₀ + K₆₀, F₄ = N₁₈₀ + P₃₀ + K₆₀ kg/ha), four levels of cutting intensity (I₁-4, I₂-3, I₃-2, early, I₄-2, late cut) and their interactive influence, i.e. a total of 16 variants on the production of forage dry matter, production of crude protein (CD) and net energy of lactation (NEL) per hectare. The dominant influence of N-nutrition is documented by significant differences in forage dry matter (DM) production between the variants (with the exception of F₁/F₂) ranging from 4.41-4.80 to 8.44-9.83 t/ha. The effect of different exploitation level on the production is subdominant and no significant differences were found either in the production of forage DM(I₁-6.41 - I₂-6.59 - I₃-6.97 - I₄-7.50 t/ha) or in the production of nutrients. Management models to be recommended for the given type of sward with respect to the interactive influence, efficacy of forage production and quality are as follows: (a) three cuts/180 kg N + PK/ha with forage suitable for dairy cows; (b) two cuts/90 kg N + PK/ha with forage suitable for cattle breeds kept for meat.

The present paper is about the provenance and family diversity of insect occurrence on the offspring of 58 pedunculate oak (Quercus robur L.) trees representing 5 populations from the Polish range of species. Researches were conducted on the Chrostowa II test plot, located in the Brzesko Forest District (southern Poland). Differences in symptoms of trees inhabitation by Andricus kollari (Hartig, 1843) ♀♀, Andricus lignicolus (Hartig, 1840) ♀♀, Andricus foecundatrix (Hartig, 1840) ♀♀, Biorrhiza pallida (Olivier, 1791) ♀♂, Andricus inflator (Hartig, 1840) ♀♂, Andricus conglomeratus (Giraud, 1859) ♀♀, Andricus quercusradicis (Fabricius, 1798) ♀♀, and Lachnus roboris (Linnaeus, 1758) were evaluated. Approximately 45% of oaks grown at the test site were damaged. The majority of trees were inhabited by A. kollari (35.7%) while the lowest number of trees was inhabited by A. inflator, A. conglomeratus and A. quercusradicis (0.1%). Analysis of variance showed that certain species prefer the offspring of certain provenances. Also a high diversity among families within provenances was found. The effect of the family and provenance was statistically significant on oaks with galls caused by A. kollari, A. foecundatrix and B. pallida share. High heritability values were obtained for A. kollari on the population and family level (0.86 and 0.70 respectively) and for the rest of the species only on the provenance level. The presence of L. roboris was greatly influenced rather by environmental than genetic factors, also being characterized by low heritability values.

Based on yield tables for oak high forest and oak coppice (both first site class) and using assortment tables and assortment prices in the Czech Republic in 2009, a set of variants of conversion of high forest to coppice was simulated. Average annual cut and average gross value of annual cut of such conversions were compared with those of well-established (in terms of the age structure balance) variants of coppice and high forest. Under the existing ratio of assortment prices, established coppice does not reach the gross value yield of high forest. No variant of simulated conversions was more financially profitable than the initial high forest. Furthermore, we found out that a +16.8% increase of the current fuel wood price would counterbalance the mean annual increment of gross value of the best coppice and the worst oak high forest variant. On the other hand, a +164.7% fuel wood price increase would be necessary to counterbalance the mean annual increment of gross value of the worst coppice and the best high forest variants.

The study on the relationship between the soil aggregates stability assessed using water stable aggregate (WSA) index and the selected terrain and soil properties was performed on a morphologically diverse study site in Chernozem soil region of Southern Moravia. Soil analyses and detailed digital elevation model processing were the main methods adopted in the study. The soil structure stability is negatively influenced by the soil material removal from the steep parts of the back-slope and re-deposition of the mineral loess material at the base of the slope. The highest aggregates stability was identified in the upper flat parts of the study plot, undisturbed by erosion processes, and at the concave parts of the back-slope with intensive accumulation of organic matter. Statistical analysis showed a significant dependence of aggregates stability on organic carbon content and plan curvature index.

Seeds of three blackgrass populations fromSouthern Bohemiawere collected in 2007-2008. Biological tests with chlorsulfuron were performed at the 2-3 leaf stage. Some plants survived after treatment with the highest dose 37.5 g/ha. Biological tests showed a resistant phenotype to chlorsulfuron. Leaves of these plants were analysed by dCAPS assay. Two domains of ALS gene: domain A ‒ P197 and domain B ‒ W574 were targeted by PCR with regenerated primers P197 containing BamHI site and W574 containing site BstXI. PCR products of all tested samples were cleaved by BamHI in the codon P197. No mutation of proline in P197 was found out. The codon W574 PCR product of the samples was not cleaved by BstXI.

The fruit of apricot (Prunus armeniaca L., Rosaceae) has been used as food in FYR Macedonia since a long time ago. The chemical organic matters from the fruit is a kind material for food processing and has potential nutritional, medical and commercial values. The results based on fruit physical and chemical analyses clearly showed that different apricot genotypes have very important contents of soluble solids, individual sugars, and titratable acidity in limited soil and climatic conditions. In addition, the contents of these chemical compounds in some genotypes were higher than those in the control cultivar Hungarian Best. Using the PC analysis (PC1 = 32.13%, PC2 = 22.86%, and PC3 = 18.32%), apricot genotypes were separated into groups with similar physical and chemical attributes. These relationships may help to select a set of genotypes with better fruit quality performances which, in our study, might be indicated in DL-1/1/04, DL-1/2/03, D-1/04 and K-5/04.

In Central Asia, stem rust (Puccinia graminis f.sp. tritici) causes considerable damage, especially during growing seasons with high rainfall. Ug99 is a race of stem rust that is virulent to the majority of wheat varieties. To develop disease-free germplasm, wheat material was screened using the predominant stem rust races of Kazakhstan and tested in two nurseries; CIMMYT-Turkey and the Plant Breeding Station at Njoro, Kenya. A total of 11 pathotypes of P. graminis f.sp. tritici were identified in Kazakhstan from the stem rust samples collected in 2008-2009. In particular, pathotypes TDT/H, TPS/H, TTH/K, TKH/R, TKT/C and TFK/R were highly virulent. Of the 170 advanced lines of wheat, 21 CIMMYT lines resistant to 5 aggressive Kazakhstani pathotypes of P. graminis were identified. A high level of resistance was observed in 11 wheat cultivars and advanced lines: Taza, E-19, E-99, E-102, E-572, E-796, E-809 (Kazakhstan), Ekinchi (Azerbaijan), Dostlik, Ulugbek 600 (Uzbekistan) and Umanka (Russia). Based on data obtained from Turkey-CIMMYT and the Plant Breeding Station Njoro, Kenya nurseries, out of 13 tested entries, 6 wheat breeding lines which were resistant to both stem and yellow rust and 10 wheat lines which showed high and moderate levels of resistance to Ug99 were selected. Using the sequence tagged site (STS) molecular marker Sr24#12, associated with Sr24/Lr24, seven wheat entries resistant to stem rust were identified. These results will assist breeders in choosing parents for crossing in programmes aimed at developing varieties with desirable levels of stem rust resistance in Kazakhstan and they will also facilitate stacking the resistance genes into advanced breeding lines.

Differences between the oak lepidopteran communities were studied in Slovakia in 1993 and 1994. Sampling was undertaken between April and October on 3 oak species. Biological traits of larvae were examined in order to explain differences in seasonal guilds among oaks. Cluster analysis shows similarities between communities in the flush feeder guild where the results were influenced mainly by temporal variability; in the late spring guild mainly by site; factors influencing summer feeder and autumn feeder similarities remain unclear. Detrended Correspondence Analysis (DCA) was used to investigate the main gradients explaining variability in communities. Results indicated that the host specificity along axis 1 (A1) and larval ballooning along axis 2 (A2) were important in the flush feeder guild; larval feeding strategy along A1 and the degree of defoliation by Lymantria dispar along A2 were important in late spring feeders; the year of study along A1 and host specificity along A2 were important in the summer feeder guild; and adaptation to lower quality of food along A1 and host specificity along A2 for the autumn feeder guild. The results are discussed.

The paper deals with environmental risk assessment in prevailingly unnatural spruce (Picea abies [L.] Karst.) forests in three regions with different patterns of forest damage in the Slovak part of the West Carpathians. Logistic regression was used to estimate the effect of 7 site-related, 5 stand-related and 2 anthropogenic factors on the probability that critical forest damage will occur. The results show that regression models can describe cause-effect relationships in regions with different regimes of forest decline. Stand age, proportion of spruce, and distance from the focus of biotic agent activity predicted decline in two regions with generally lower elevation in northern Slovakia (Kysuce and Orava). In a mountain region (Low Tatras), the importance of factors contributing to the static stability of trees and position towards dangerous winds increased significantly. The quality of the derived models and prospects for their usefulness in risk assessment are discussed.

The aim of this study was to investigate tree-ring width variability and to distinguish groups of trees with similar growth trends in order to study tree growth responses to various stand and site conditions. The methods of cluster analysis were employed for this purpose. Four distinct groups of trees were identified. For each group, the mean tree-ring curve was calculated in order to look for the main signals that distinguish the groups from one another. The idea behind this was to divide the samples into homogeneous groups with similar growth trends, representing typical examples of variability of the studied Norway spruce population. In the next step, several regression functions were studied and compared for their ability to fit the ring-width-age data applied to the mean ring-width curve of each group. Fischer's F-test was used to test the differences in goodness of fit between the equations in each group. From all examined/applied equations, smoothing spline, polynomial of degree 5, and Šmelko-Burgan functions were found to be the most universal and suitable for detrending of all examined ring width curves. Hugershoff function was found to be suitable for curves with one local maximum only. Exponential and Korf's functions were unsatisfactory for the purposes of tree ring curves detrending.

In precise field trials in the CR in the years 2004 and 2005 the effect of site conditions, yellow- and purple-fleshed varieties and fertilization on the content of total polyphenols (TP) in potato tubers was investigated. Comparing four localities, significantly highest TP content (by 5.7 to 13.4% higher than in other localities) was determined in the locality Stachy in both years, which we ascribe to apparently lower temperatures in the vegetation period in this locality of high altitude. In comparison to yellow flesh varieties, in the case of purple flesh Valfi variety TP content higher by 52 to 153% was recorded as the average of both years. As for the group of eight yellow flesh varieties, Karin variety had the highest TP content and showed a significant increase in comparison to Agria, Saturna and Asterix varieties by 67, 60 and 37%, respectively. TP content was not demonstrably affected by fertilization with mineral fertilizers, but we recorded a tendency to lesser TP content (by 3 to 9.9%) in the variant with higher potassium and magnesium fertilization (166 kg K/ha and 60 kg Mg/ha).

Fertilization of crops with pig manure is a common practice throughout the world. Nevertheless, due to the relatively high copper (Cu) and zinc (Zn) contents in pig manure, continuous application of pig manure could have negative effects on soil and plant. The study aimed at the impacts of long-term applying different pig manure rates (equivalently 0, 100, 250 and 500 kg total N/ha/year from 2002 to 2008 and 0, 10, 25 and 50 t fresh weight/ha/year from 2009 to 2011, respectively) on Cu and Zn accumulation in soil and plant. During the 10 years of the experiment, a total of 2.04 to 10.20 kg/ha/year for Cu, 3.15 to 15.73 kg/ha/year for Zn were applied to the soil. Results from this study showed that long-term pig manure application resulted in serious accumulation of Cu and Zn in soil, total Cu and Zn concentrations increased by 204% and 107% at high application rates, respectively. Although topsoil Cu and Zn concentrations were below concentrations considered phytotoxic to crops, according to current Chinese legislation, it would take only less time than 16 and 27 years of high application rates to reach the allowable limits. Our result also suggested that Cu and Zn leaching occurred in the tested soil. The Cu and Zn concentrations in stalks and grains were not affected by the application of pig manure, and these values were lower than the threshold values for animal and human ingestion.

A three-year-old male western black-tailed rattlesnake (Crotalus molossus molossus), which was refusing food and losing weight was presented with a swelling on the right side of the head below the eye. An abscess was suspected and treatment with subcutaneous enrofloxacin was started immediately. After identification of the causative agents as Pseudomonas aeruginosa and Stenotrophomonas maltophilia, and following susceptibility testing, enrofloxacin treatment was replaced with marbofloxacin, intralesional gentamicin/betamethasone treatment and gentamicin administered subcutaneously at an increased terrarium temperature of 35 °C. Seven days later, the formed pus plug was debrided and a combined marbofloxacin/gentamicin/betamethasone treatment was continued for an additional seven days. The swelling disappeared. Marbofloxacin was continued for ten more days, after which time microbiology tests were negative for S. maltophilia and P. aeruginosa. The animal began to eat and gain body weight. To our knowledge, this is the first report of an abscess treatment in a rattlesnake and the first to demonstrate the effective treatment of a S. maltophilia-induced infection with a combination of marbofloxacin and gentamicin.

Tumours of the mammary gland were diagnosed in one female Djungarian hamster (Phodopus sungaros) and in two of her daughters from two litters. Altogether, five tumours were diagnosed. Three of them were adenocarcinomas, one was adenoma with disseminated foci of adenocarcinoma and one was diagnosed as an atypical fibrosarcoma derived from cutaneous ganglion cell-like cells. This was a recurrent tumour in the proximity of the previously extirpated adenocarcinoma. Using specific PCR primers for conserved regions of the mouse mammary tumour virus (MMTV) no endogenous provirus DNA could be detected in DNA samples isolated from formalin-fixed paraffin embedded tissue sections.

Thirty-nine multiparous ewes (19 Pelibuey and 20 Romanov × Pelibuey) treated with fluorogestone acetate impregnated intravaginal sponges were used to evaluate the effects of low pregnant mare serum gonadotropin (PMSG) doses and genotype on their reproductive efficiency under heat stress conditions. The sponge treatment lasted for 12 days, and 24 h before sponge removal, ewes of each genotype were injected with 140 or 280 IU of PMSG. Ewes showing estrus were naturally mated twice. Reproductive performance was not affected (P > 0.05) by the dose × genotype interaction. All treated ewes presented estrus signs within a 48-h period after sponge removal. Shorter (P < 0.05) estrus interval and higher (P < 0.05) fecundity were observed in ewes treated with 280 IU of PMSG compared to those treated with 140 IU. Pelibuey ewes exhibited shorter (P < 0.01) estrus interval and greater (P < 0.01) fertility as compared with Romanov × Pelibuey ewes. The response to estrus, gestation length, prolificacy, and percentage of single and multiple lambing were not affected (P > 0.05) by dose or genotype. In conclusion, under heat stress conditions, low PMSG doses as 140 or 280 IU can be used to successfully induce and/or synchronize the estrus in Pelibuey ewes and their crosses with Romanov, regardless of reduced fertility observed in crossed Pelibuey ewes. If a more predictable and compact estrus is required, administration of 280 IU of PMSG is recommended.

Increasing productivity and reducing cost in long-distance transportation have become increasingly important in the logistics of forest harvesting operations. This paper presents the research results of the performance of loading with Volvo 4500 BM and timber trucking using two types of common trucks in the Nav watershed in Iran. Both trucks can carry logs shorter than 7.8 m in length. The study used a time study based on empirical data for loads collected from one procurement district in the Nav watershed. The models included the following explanatory factors: driving distance, number of logs, and load volume. The time consumption and productivity of loading and timber trucking depends on several variables such as volume and number of logs per cycle. To evaluate the current transportation system in the Nav watershed, the empirical time study was conducted. Since transportation includes several phases and since many factors affect the work performance, significant variation in the total transportation time was observed. This makes planning and cost accounting more difficult. The models developed in the study are a promising way to support route planning and optimization, and cost and profitability calculation for trucking en­trepreneurs and the forest industry. The average productivity of log transportation was 2.84 and 3.4 m3.effective h-1 for the dump truck and chassis truck, respectively. The average hauling unit cost was 18 and 15 USD.m-3 in the dump truck and chassis truck, respectively.

Root net primary productivity (RNPP) was assessed in the wet submontane meadow in the highland region of the Czech Republic. RNPP was studied from 1992 to 1995 with help of the in-growth core technique. The effect of different intensities of fertilization on root growth was covered. In comparison with unfertilized stands, the application of fertilizers (90 kg N/ha) resulted, mostly significantly, in greater root productivity (297 g/m²/year and 2.08 g/m²/day, on average). This represented an increase by 72% and 71%, respectively. Variability in the fraction of RNPP to total net primary productivity was examined. Results indicate that this fraction varied from 0.18 to 0.25 across the compared treatments and decreased with increasing fertilization. Data show how different roles can grasslands play in accumulation of plant matter due to different levels of fertilization.

The paper is the 6^th report on the production potential and stability of mixed forest stands in uplands. A mixed beech/larch stand that was established by natural regeneration in 1934 to 1942 is assessed. The stand is situated at an altitude of 460 m above sea level. It has been left to its natural development since 1961. At that time, the stand was characterized as an individually mixed, diameter- and height-differentiated 25-year pole-stage stand. The proportion of larch and beech amounted to 40% and 17%, respectively. Hornbeam (25%), oak (11%) and to a lesser extent birch (5%) and spruce (3%) also occurred in the stand. In the course of 42 years, the proportion of larch in this stand without planned thinning measures decreased to 35%. On the other hand, the proportion of beech increased to 39%. During all 5-year inventories, the stand could be characterized as a stabilized one with high production potential. Its initial growing stock 63 m³/ha at an age of 25 years increased to 497 m³/ha at an age of 67 years in 2003. At present, current volume increment amounts to 9.8 to 12.5 m³/ha/year.

Biochar (BC), the carbon-rich by-product resulting from pyrolysis of biomass, is used for bioenergy and increasingly as a soil additive for carbon sequestration and soil improvement. However, information about the effects of BC on forest productivity and reforestation success, especially on boreal and temperate forest soils, is scant. We examined the effects of two BC types (Canadian and Finnish) added in proportions up to 60 vol.% into a common alluvial silty soil on the growth of transplanted Norway spruce (Picea abies [L.] Karst.) seedlings in a growth chamber. We found no marked differences in seedling growth among the binary growing media mixes used. Seedling growth attributes (seedling height, terminal shoot growth, root volume) differed consistently only between the BC types in the highest proportion used. The terminal shoot growth differed overall among the two BC types. These results suggest that BC may be applied into mineral soils without detrimental chemical effects on tree plantation success. Our results provide foundation for further field research on the longer-term impacts of adding BCs to boreal forest soils.

Only two cambioxylophagous species (Ips amitinus and Pityogenes chalcographus) were found on sections of Picea pungens and Picea abies that belong to economically important pests of Norway spruce. Representatives of the Isarthron sp. (Cerambycidae) were developed on the examined sections of both P. abies and P. pungens. The monitoring of attractiveness of the spruce species P. pungens and P. abies to cambioxylophages revealed that P. pungens was more intensively attacked by I. amitinus while P. chalcographus was more abundant on P. abies. Both bark beetle species preferred shaded parts of tree trunks, although their development was more successful on the sun-exposed side. On P. pungens, abundance of P. chalcographus decreased and abundance of I. amitinus increased with increasing trunk volume. Blue spruce was more attractive to Isarthron sp. compared to Norway spruce.

Plant's nitrogen (N) requirement that is not fulfilled by available N in soil has to be supplied externally through chemical fertilizers. A reliable estimate of soil N-supplying capacity (NSC) is therefore essential for efficient fertilizer use. In this study involving a pot experiment with twenty acidic soils varying widely in properties, we evaluated six chemical indices of soil N-availability viz. organic carbon (C_org), total N (N_tot), acid and alkaline-KMnO₄ extractable-N, hot KCl extractable-N (KCl-N) and phosphate-borate buffer extractable-N (PBB-N), based on their strength of correlation with available-N values obtained through aerobic incubation (AI-N) and anaerobic incubation (ANI-N), and also with the dry matter yield (DMY), N percentage and plant (maize) N uptake (PNU). In general, the soils showed large variability in NSC as indicated by variability in PNU which ranged from 598 to 1026 mg/pot. Correlations of the N-availability indices with AI-N and ANI-N decreased in the order: PBB-N (r = 0.784** and 0.901**) > KCl-N (r = 0.773** and 0.743**) > acid KMnO₄-N (r = 0.575** and 0.651**) ≥ C_org (r = 0.591** and 0.531**) ≥ alkaline KMnO₄-N (r = 0.394** and 0.548**) > N_tot (r = 0.297** and 0.273*). Of all the indices evaluated, PBB-N showed the best correlations with plant parameters as well (r = 0.790** and 0.793** for DMY and PNU, respectively). Based on the highest correlations of PBB-N with biological indices as well as plant responses, we propose PBB-N as an appropriate index of N-availability in the acidic soils of India and other regions with similar soils.

Soil samples were collected at both jointing and maturing stages of maize and wheat to compare the effects of 4-year no-tillage (NT) and conventional tillage (CT) on seasonal variations of microbial biomass carbon (C), metabolic quotient, and invertase activity in a sandy loam soil in North China. Soil invertase activity significantly increased (P < 0.05) from summer to spring of the next year and then significantly decreased (P < 0.05) from spring to summer. With a delay of about 3 months, soil microbial biomass C and basal respiration altered in a similar pattern, while microbial metabolic quotient changed on the contrary. Compared with CT, the NT practice significantly increased (P < 0.05) soil organic C content, and tended to result in higher soil microbial biomass C and invertase activity, as well as lower soil microbial metabolic quotient, especially at the jointing stage of maize. Our results indicated that NT might play an important role in the improvement of soil microbial efficiency, especially at the maize seedling season.

To investigate the environmental advantages of using grass-clover binary mixtures over pure stands as winter cover crops, a serial of five field experiments (each designed as randomized complete blocks with four replicates) was carried out in eastern Slovenia. Trifolium incarnatum L. and Lolium multiflorum Lam. were sown in late summer as pure stands and binary mixtures. Pooled data calculated from all the experiments revealed that the soil mineral N in spring and accumulation of N by plants decreased with decreasing proportion of T. incarnatum in the binary mixtures, while the C:N ratio of cover crop organic matter increased. C accumulation was the highest when the seeding ratio of the binary mixture of T. incarnatum and L. multiflorum was 50:50. In the C and N environmentally sustainable management efficiency coefficients, three important traits of winter cover crops for environmental pro-tection were given equal importance (low soil mineral N content in spring, high C accumulation in plants, and high N accumulation in plants). The coefficient was higher for binary mixtures of T. incarnatum and L. multiflorum than for pure stands of these crops, proving the complex environmental advantages of binary mixtures over pure stands.

Essential oils (EOs) are important aromatic components of herbs and spices and their biological activities have been known and utilised since ancient times in perfumery, food preservation, flavouring, and medicine. Some of their biological activities include antibacterial, antifungal, anti-oxidant and anti-inflammatory effects amongst others. EOs have received attention in recent years as potential 'natural' alternatives for replacing antibiotic growth promoters (AGPs) in animal diets due to their positive impact on growth performance, gut microbiota and welfare. The purpose of this paper is to provide an overview of our own published and unpublished data on the antibacterial, antifungal and insecticidal activity of thymol and cinnamaldehyde (TC blend), and to describe the effects of this specific EO blend on gut microbiota, growth performance and welfare, carcass characteristics and food safety. The possible modes of action of EOs are discussed and areas for future research are proposed.

Interest in conifer-broadleaf mixedwood forests has greatly increased due to continuous demand for hardwood products and a shift towards more biological or ecosystem-based management. In British Columbia, more than 30% of the productive forest land is a conifer-broadleaf mixture and current forest regulations are more conifer biased rather than maintaining a mixed-species condition. The aim of this study was to examine the impact of paper birch on white spruce growth. Spruce growth data from 10 to 18 years old complex stands indicate that radial, height, and stem volume was not impacted by retaining up to 3,000 stems.ha^-1 of birch. Similarly, growth and yield model projections suggest spruce-birch stands would be more productive up to a threshold birch density (3,000 stems.ha^-1) than pure spruce stands. At a 4% real interest rate, the removal of birch from these stands does not appear to be warranted as an investment. The results suggest that instead of encouraging uniform broadleaf removal across conifer plantations, mixed species management strategies could enhance the forest productivity, stand diversity and resilience.

A field study was conducted to ascertain the effect of three zinc (Zn) levels: 0, 20 kg ZnSO₄/ha and 20 kg ZnSO₄/ha + foliar spray of 0.5% ZnSO₄, on wheat grain Zn content and factors contributing to or hindering in its bioavailability. Increasing Zn levels were established as serviceable in improving the nutritional status of genotypes. Soil application + foliar spray proved to be paramount for all the traits leading to an 80% increase in grain Zn content, 61.3% in methionine content and a decrease of 23.2% in phytic acid as an average of all genotypes and both years. The genotype UP 2382 was found more suited to Zn fertilization in allocating Zn and maintaining a lower phytate to Zn molar ratio.