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Changes in genetic diversity of peas bred in the Czech Republic and in former Czechoslovakia since the mid-20^th century were analysed using 38 molecular marker loci, including retrotransposons and microsatellites, differentiating a total of 84 alleles. Both marker types were comparably effective in revealing the genetic diversity, with a high correlation (r = 0.81), although the pairwise genetic distances of each marker type differed. In total, 175 accessions, selected from the Czech pea gene bank collection and representing the pea cultivars collected or bred in the country, were divided into three groups according to their date of sampling or variety registration. The first group contained 70 old cultivars and landraces collected prior to 1961. The second group contained 46 cultivars released from 1961 to 1980. The third group contained 59 cultivars released between 1981 and 2004. In spite of the decline in several diversity measures, differences in allele frequencies and even allele loss in three microsatellite loci were recorded over the 70-year period, while these differences between the groups were not statistically significant. In addition, genetic heterogeneity was detected in 29 accessions (15%). This indicates that although no genetic erosion could be observed since then, it is important to monitor the genetic diversity, furthermore it highlights the vital role of germplasm collections for the crop diversity conservation.

The literature linking Bt maize versus non-Bt maize and the changes in the fungal microflora spectrum and in the mycotoxins content have been summarised. The European corn borer reportedly promotes the infection of maize by Fusarium spp. Stalk and ear rots caused by Fusarium spp. are often related to mycotoxin accumulation in maize kernels. As a result, food and animal feed from maize are more severely contaminated with Fusarium mycotoxins: e.g. fumonisins (FUM), deoxynivalenol (DON), and zearalenone (ZEA). Mycotoxins in field maize lead annually economic losses of hundreds of millions of dollars in all regions of the world. The insecticidal proteins in genetically modified hybrid Bt maize reduce insect damage caused by certain Lepidopteran larvae, which in turn can reduce the infection of the grain by the mycotoxigenic fungi. Where such insect damage is a major factor in mycotoxin contamination, Bt maize can lower mycotoxin levels in many cases. The protection of maize plants against insect damage (European corn borer) through the use of Bt technology seems to be one of the ways to reduce the contamination of maize by Fusarium species and mycotoxins.

Soil arsenic (As) levels are particularly high in parts of China, where wheat and rape are widely grown. Understanding the effects of As concentration on the growth of these two major crops is of significance for food production and security in China. A pot experiment was carried out to study the uptake of As and phosphorus (P), and the soil As bioavailability at different growth stages of wheat and rape. The results indicated that winter wheat was much more sensitive to As stress than rape. Wheat yields were elevated at low rates of As addition (< 60 mg/kg) but reduced at high rates of As concentrations (80-100 mg/kg); while the growth of rape hadn't showed significant responses to As addition. Phosphorus concentrations in wheat at jointing and ear sprouting stages increased with increasing soil As concentrations, and these increases were assumed to contribute a lot to enhanced growth of wheat at low As treatments. Arsenic did not significantly affect P concentrations in rape either. The highest As concentrations in wheat shoot and rape leaf were 8.31 and 3.63 mg/kg, respectively. Arsenic concentrations in wheat and rape grains did not exceed the maximum permissible limit for food stuffs of 1.0 mg/kg. When soil As concentration was less than 60 mg/kg, both wheat and rape could grow satisfactorily without adverse effects; when soil As concentration was 80-100 mg/kg, rape was more suitable to be planted than wheat.

The present study was undertaken to quantify changes in the status of soil nutrients, their depletion and build-up after continuous long intensive cropping for last 36 years in a permanent manorial trial which has been in progress since 1972 in an alfisol of western Himalayas. The rotation was maize-wheat which included various combinations of N, P, K, Zn and FYM (farmyard manure). Continuous cultivation influenced pH, OC (organic carbon), available N (nitrogen), P (phosphorus) and K (potassium). An increase in the status of organic carbon was observed in 100% NPK + FYM treatments for more than three decades from initial value of 7.9 to 12.0 g/kg. The use of either FYM or lime alongwith 100% NPK sustained crop productivity or improved nutrient status. However, imbalanced use of nutrients i.e. NP or N alone is adversely affecting the fertility of soil by aggravating the problem of soil acidity. Application of S free P fertilizer DAP (diammonium phosphate) drastically reduced the yield of both the crops. Thus, continuous use of balanced fertilizers is necessary for sustaining soil fertility and productivity of crops.

The aim of the study was to examine for the first time the various aspects of canine atopic dermatitis (CAD) presenting in a Middle Eastern country. Medical records of 164 dogs diagnosed with CAD were evaluated. Associations between signalment, lifestyle, clinical signs and allergens were evaluated statistically. Labrador Retriever, German Shepherd dog, Boxer, French Bulldog, Golden Retriever and Shar-Pei breeds were presented more frequently than the regular hospital population (P < 0.0001), and had an earlier disease onset time (P < 0.01). In 22 dogs (13%) signs of CAD were noticed at less than six months of age. Most dogs (75%) lived indoors. Most dogs (83%) had lesions on the ventral part of the body and 68% had foot lesions. After excluding flea allergy dermatitis by implementing strict flea control, 60% of the dogs presented with dorsal distribution of skin lesions. Dogs with ventral lesions were younger when clinical signs first appeared (P < 0.05). Most of the dogs were allergic to dust and/or dust mites (75.6%), with weeds and trees as the next most common allergens. CAD is similar worldwide, but geographic differences may be attributable to genetic pools and allergen loading. This study also shows that early onset of clinical signs, especially in breeds predisposed to CAD and with a dorsal distribution of skin lesions, should not rule out the diagnosis of CAD.

This paper presents research results of the performance of HSM-904 grapple skidder using a full tree harvesting method in a non-native aspen Populus deltoides plantation in even terrain conditions in Shafaroud, Northern Iran. Patch cutting as a silvicultural method was used in the plantation (2,000 ha). To evaluate the newly introduced machine to a ground skidding system in the aspen plantation and the possibility to increase the production rate, an empirical time study was conducted. The elements of the skidding work phase were identified and 58 cycles were recorded for the study. The models for effective time consumption, total productivity and work phase models are calculated. The average load per cycle was 2.2 m³, the average one-way skidding distance was 253 m. The average travel speed of unloaded skidder was 5.53 km.h^-1 and the average speed of loaded skidder was lower than the speed of the unloaded one by 2.94 km.h^-1. The average output in the study was 7.1 m³ per effective hour and the unit cost was 13.9 USD.m^-3.

Field experiments were conducted at two different locations (Barani Agricultural Research Institute Chakwal and farm field Talagang, district Chakwal) for two crop-growing seasons in northern rainfed Punjab, Pakistan to assess the yield and micronutrient uptake of chickpea (Cicer arietinum). The treatments were four combinations of two levels of sulfur (15 and 30 kg/ha) from two sources (gypsum and ammonium sulfate) and a no-sulfur control. Application of sulfur resulted in a significant increase in seed yield up to 17% over control. Ammonium sulfate was a more efficient source of sulfur as compared to gypsum at both the locations. Sulfur application resulted in a significant increase in micronutrient uptake by plant; however effect of sulfur application on soil pH at the end of experiment was not significant. Availability of soil zinc and copper increased with sulfur application at the end of two year experiment. Tissue copper and iron and soil available copper and iron correlated negatively with soil pH. Sulfur should be applied to chickpea grown under rainfed conditions in order to increase seed yield, to improve nutritional composition of product and to enhance efficiency of other fertilizers.

A three-year-old intact female Great Pyrenees underwent total knee replacement for the treatment of osteoarthritis. The dog contracted septic arthritis 10 weeks following the procedure. Treatments including implant subtraction, debridement of tissue, and insertion of bone cement with antibiotics into the joint space were completed in the joint cavity. A temporary external skeletal fixator was used to stabilise the joint for four weeks. A second attempt at total knee replacement failed due to severe muscle contracture and bone loss. Therefore, it was decided to perform arthrodesis as a salvage procedure. At the 17 month follow-up examination, the patient was able to use the limb while standing and at all gait speeds. Based on these findings, two-stage arthrodesis can be a viable salvage procedure for infected total knee replacements.

The objective of the study was to determine the relationships between age of the goat and faecal egg counts, liver enzymes, and minerals in the wet and dry seasons in male and female Nguni goats of South Africa. Fifty-six female and forty male Nguni goats were used for the study. Faecal and blood samples were collected once in the dry (August) and wet (January) season. Faecal egg counts (FEC) were determined by the modified McMaster technique while trematodes were determined by the sedimentation method. Blood was analyzed for phosphorus, calcium, magnesium, alkaline phosphatase (ALP), alanine transaminase (ALT), creatine kinase (CK), aspartate aminotransferase (AST), and gamma-glutamyltransferase (GGT) levels. Faecal egg counts were significantly higher in the wet compared with the dry season. Most (P < 0.05) of the goats were within the reference values for calcium, phosphorus, and magnesium in both seasons. Phosphorus concentrations were significantly affected by age with higher levels in the young (2.1 ± 0.06) compared to the adult (2.0 ± 0.03) goats. Alkaline phosphate was significantly affected by age with higher levels in young than in mature goats. Higher AST, CK, and GGT concentrations were recorded in the wet compared to the dry season (P < 0.05). Higher CK and AST were recorded in male than in female goats while for ALP, the values were higher in female than in male goats. Linear negative relationships (P < 0.05) existed between age and ALP, phosphorus, and FEC, while quadratic relationships existed between age and strongyles and Strongyloides egg counts. Calcium was linearly related (P < 0.05) to FEC, while CK was related to FEC in a quadratic fashion. Age of the Nguni goats can be used to predict faecal egg counts, phosphorus, and ALP levels.

The effect of Scots pine admixture in European beech stand on the leaching of dissolved organic carbon (DOC), dissolved organic nitrogen (DON), nitrate nitrogen (NO₃-N) and ammonium nitrogen (NH₄-N) from organic and humic horizons of Dystric Arenosols was studied in northern Poland in 2008-2009. Three zero-tension lysimeters under organic and humic horizons were installed in pure beech and mixed pine-beech stands. Water samples were collected after each rainfall, measured volumetrically, filtered and analysed. In each sample pH and concentrations of DOC, DON, NH₄-N and NO₃-N were analysed. Stronger acidification of leachates was observed in mixed stand compared to pure beech. About twice higher concentration of DOC and its fluxes per unit area were determined in mixed stand. The fluxes of DOC from unit mass of soil were less varied. In general, lower concentrations of DON, NH₄-N and NO₃-N as well as fluxes of the components (calculated in mg.kg^-1DM.year^-1 and mg.m^-2.year^-1) were observed in mixed stand.

32 experimental plots were sown at Borovce (district Trnava) in Slovakia during 2008 with 8 maize hybrids, including coleopteran-protected MON 88017, the lepidopteran-protected MON 89034, the stacked product, MON 89034 × MON 88017, that same hybrids isoline that did not have the Bt trait or traits, and 4 commercial hybrids. Part of the fourth repetition was unexpectedly infested and damaged with the Western corn rootworm (WCR). High numbers of the WCR adults were found in the damaged plots on July 8. Adult WCR abundance remained concentrated in this area until August 12-19. The beetles apparently began to disperse during August 19-September 2, and after September 2. The trap captures increased in the uninfested areas up to September 16, no significant difference having been found in the adult WCR captures between the infested and uninfested plots, including the plots with MON 88017. Bt-maize hybrids containing MON 88017 strongly influenced the level of the WCR damage as assessed by lodging of the maize plants, and they also influenced the numbers of the WCR adults before maize flowering. We found that intensive movement of the WCR adults from the place of their origin started later in the vegetation season.

A field experiment was conducted to illustrate the different degree and dynamics of microbial community structure and function in the rhizosphere across four growing stages (before plantation and three growth stages) using a combination of biochemical (enzyme assay and microbial biomass carbon) and molecular approaches of qPCR and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis). Rice plant cultivation promoted higher enzyme activities (invertase and urease), microbial biomass carbon (C_mic), bacterial (16S rRNA) and fungal (ITS rRNA) genes abundances in the rhizosphere compared to unplanted soil. Principal component analyses of PCR-DGGE profile also revealed that structures of bacterial and fungal communities of rice planted soil were well distinct from unplanted soil. Moreover, enzyme activities showed a significant positive correlation with the total microbial biomass in the rhizosphere throughout growth stages of rice plant. Relative fungal: bacterial ratios were significantly higher in rice planted soil compared to unplanted soil, suggesting rice plantation enhanced the fungal community in the rice rhizosphere environment. These results further suggest a significant linkage between the microbial community dynamics and function in the rhizosphere associated with rice plant over time.

The present study was conducted to verify the effect of Ceylon cinnamon on experimentally induced excision wounds in rats. Thirty-two rats were divided into four groups of eight rats each. Group A received a placebo containing 1.5% of cinnamon and Group B a placebo containing 3%. Group C, as the control group, didn't receive any treatment and finally Group D received a blank placebo as the reference standard group. Wound healing was monitored on Days 3, 6, 9 and 14 and histological evaluation was carried out on the samples. The results show that cinnamon extract served to accelerate the wound healing process and specifically increased epithelialization in treatment groups compared to the other groups. Thus, this study demonstrates that Ceylon cinnamon may be effective in stimulating the enclosure of wounds.

The leaf spotting disease complex is a major biotic constrain in enhancing grain production in the major wheat growing regions. Two leaf spotting diseases, tan spot, caused by an ascomycete fungus Pyrenophora tritici-repentis, and Stagonospora nodorum blotch besides causing average yield losses of 5-10%, cause significant losses in grain quality by red smudge, black point and grain shriveling. Conservation agriculture in combination with wheat monoculture involving cultivation of susceptible cultivars has resulted in frequent onset of leaf spots epidemics worldwide. Development of resistant wheat cultivars, in conjunction with crop rotation, will provide an effective, economical, and environmentally safe means of controlling leaf spot. International Maize and Wheat Improvement Center (CIMMYT), Mexico has initiated major efforts to mitigate the threat of tan spot. Efforts include screening of wheat germplasm, identification of new sources of resistance, characterization of new tan spot resistance genes through classical and molecular genetic analysis, incorporation of resistance into adapted cultivars, and assessing the variability in the tan spot fungus. Screening studies reveal that elite CIMMYT germplasm has high level of resistance to tan spot caused by P. tritici-repentis race 1. These germplasm have diverse genetic make-up and the resistance is likely broad based. Association mapping studies done with CIMMYT germplasm reconfirmed the presence of previously identified genomic regions for tan spot resistance; however, novel genomic regions on long arm of chromosomes 6A and 7B have also been identified. Studies done to date indicate that CIMMYT germplasm possess high level diverse genetic based resistance to tan spot of wheat. Efforts are in place to develop desired wheat cultivars with tan spot resistance. Virulence studies indicate presence of P. tritici-repentis race 1 only with some variability in level of toxin Ptr ToxA produced in each of the 76 isolates studied.

Soil quality and fertility are associated with its productivity, and this in turn is connected to the soil biological activity. To study these effects, well designed long-term field experiments that provide comprehensive data sets are the most applicable. Four treatments (tillage methods) were set up: (1) conventional tillage (CT); (2) no tillage (NT); (3) minimum tillage + straw (MTS), and (4) no tillage + mulch (NTM). Our objective was to assess the relationships between soil microbial characteristics and winter wheat yields under these different techniques of conservation tillage within a field experiment, originally established in 1995. The differences in average grain yields over time period 2002-2009 between the variants were not statistically significant. Organic carbon in the topsoil was higher in plots with conservation tillage (NT, MTS, and NTM), than in the conventional tillage plots. There was a statistically significant correlation (P ≤ 0.01) between the grain yields and organic C content in topsoil.

This study was undertaken in order to test whether the development of nodule density over the vegetation period is different in lucerne stands grown for 1, 2 or 3 years continuously. For rapidly assessing nodule density in the field, a modified profile wall method was applied. Nodules were counted on a vertical profile wall, after spraying away a 2 cm layer of soil. For validating this method nodule density was determined on roots washed from monolith samples. Field data indicate that there is a shift of nodulation towards deeper soil layers with increasing maturity of lucerne stands. In 1-year lucerne nodulation was limited virtually to the top 15 cm of soil. In the 15-30 cm soil layer and in the subsoil (30-80 cm), nodule density increased with the cropping duration (1 year < 2 years < 3 years). Temporal decreases in nodule density during the vegetation period associated with dry spells were more pronounced for 2-years as compared with 3-years lucerne.

A canine patient with osteosarcoma of the sacrum was subjected to low-field magnetic resonance imaging with the following sequences: FSE, SE, 3D HYCE, X BONE, Fast STIR, GE STIR and Gradient Echo. Due to the size and location of the tumour, surgery was not performed, and the patient was euthanized. A histopathological analysis of the tumour revealed telangiectatic osteosarcoma.

In this study, productivity rate and operation cost of farm tractor were evaluated in a mountainous natural forest. Data for the study came from a detailed time study in the Research and Educational Forest of Tarbiat Modares University (REFTMU) and was used to develop a multiple linear regression model to predict the skidding cycle time. The results showed that effective independent variables of skidding time were skidding distance and slope of skid trail (P = 0.01). Average productivity rate ranged from 2.43 to 2.60 m².h^-1. Total time and effective time, respectively. Total cost of the system was 10.24 USD.h^-1 whereas 4.58% of the cost of skidding resulted from personal delay. The cost and productivity resulting from this study emphasized the importance for foresters to consider a farm tractor when designing skidding operations in young stands or prescribing a combination of two machines in mature stands.

The objective of the study was to evaluate the growth potential of Carpathian birch (Betula carpatica W. et K.) at an environmentally harsh mountain site and a response of this species to altered soil chemistry after dolomitic limestone and basalt grit applications. The Carpathian birch proved to be a suitable species for the replanting of extreme acidic mountain sites. This birch shows a low mortality rate, grows well in the clear-felled patches and soon forms a cover which is necessary for the reintroducing of more sensitive tree species. The application of dolomitic limestone and basalt grit resulted in the slower growth of Carpathian birch plantations. Liming raised soil reaction, sum of exchangeable bases, base saturation, cation exchange capacity and reduced exchangeable Al content. On the other hand, liming decreased an amount of oxidizable soil organic matter and negatively affected soil N, exchangeable P and K. Basalt grit increased exchangeable P and K contents and raised soil reaction, however only slightly. The influence of basalt grit on the sum of exchangeable bases, base saturation and cation exchange capacity was also less pronounced compared to liming. Basalt grit elevated the proportion of exchangeable aluminium and reduced the content of soil N.

Over 2004-2006, effects of mineral fertilisers (60 N + 30 P + 60 K kg/ha or 120 N + 30 P + 60 K kg/ha) and farmyard manure (12 or 24 t/ha) application on biological and chemical properties of soil were studied in seminatural grassland. The research trial was established at Radvaň site, central Slovakia (altitude 480 m, loamy to sandy-loamy Cambisol). These parameters were investigated during the growing seasons: CO₂ production, intensity of total N mineralisation (TMN), the rate of nitrification (NIT), total C content in microbial biomass (MB-C), C_ox, N_t, P, K, Mg and the humic acids to fulvic acids ratio (HA:FA). At the application of mineral fertilisers, the highest N rate significantly increased TMN and NIT, mainly in 2004 and 2005. The manure application showed the strongest effects on the biological parameters. The manure rate of 12 t/ha significantly increased the soil respiration in 2005 and 2006 as well as TMN and NIT throughout the research. The manure application increased also the soil pH, MB-C, N_t, Mg, P and C_ox.

Freeze-dried cortical bone can be used as a biological plate, either alone or in combination with other internal fixation devices, to stabilize fractures. In addition to it conferring mechanical stability, freeze-dried cortical bone may enhance fracture-healing and increase the bone stock. This study examined the effect of a freeze-dried allograft cortical bone plate (FACBP) on the biomechanical properties of an implant site in a canine bone defect model. Twelve adult mongrel dogs (around 4.8 kg) were used. A segmental critical-size defect (5 mm in length) at ulna diaphysis was created using an oscillating saw. The experimental animals were divided into two groups: eight dogs treated with an absorbable bone plate (FACBP) fixed by metal bone screws (Group A) and four dogs treated with a commercial stainless steel bone plate and metal bone screws (Group B). Bone healing was assessed by radiography, Dual-energy x-ray absorptiometry and a three-point bending test. The FACBP incorporated in the host bone produced complete remodeling of the cortical bone. There was no significant difference in the bone mineral density and biomechanical tests between the FACBP application site and normal ulna or a stainless steel bone plate of the ulna. These results suggest that FACBP facilitates recovery from a bone fracture by assisting in the induction of new bone formation in a defected fracture.

: For the measurement of the infiltration speed under operational conditions, we were equipped by a rain simulator with the measuring surface of 0.5 m². The infiltration speed is determined from the defined rain intensity and water surface runoff from the measured surface. The retained water mass from the surface runoff is recorded at regular time intervals over the whole measuring period. The beginning of the water runoff from the measured surface indicates the beginning of elutriation. The measuring time is finished after the infiltration speed has been stabilised. The beginning of elutriation and infiltration speed stabilisation are typical and mutually comparable parameters for defined soil properties at the site followed.

Spirocerca lupi is accepted in Veterinary Medicine as the cause of true malignancies in dogs. Aortic lesions and esophageal nodular granulomas are pathological lesions of spirocercosis that are considered to be pathognomic for this disease. Post mortem and histopathological studies of 105 stray dogs both male and female of different age groups from various residential areas were performed and their esophagus were examined for any lesions and helminthic parasites. Twenty dogs (19.04%) were infected with S. lupi. The infection rate was significantly higher in dogs greater than one year old (23.5%) and in western areas (36.4%) with similar prevalence rate in males and females. At histopathologic level, two isolate but closely related fibrosarcoma masses were situated at the distal portion of the thoracic esophagus of one dog and in the site of junction of esophagus and stomach of another one. Metastasis were observed in the lung and mediastinal lymph nodes of one of the dogs. Despite presence of large population of stray dogs little information is available about S. lupi in this country, so this study was undertaken to obtain an overall picture of this infection in stray dogs of Shiraz area, southern Iran.

The work aimed to evaluate the state of agricultural soil contamination what is important for the gaining of information needed for growing of hygienic safe raw materials and foodstuffs. Metallic pollution of soil in Zemplínska polluted area has begins by accumulation of heavy metals in soil, mainly resulting from location in vicinity of chemical and industrial factories, as well as from many others sources. The pH value development indicates gradual trend of soils acidification, except of alkalic ones reaching up to 20% from total arable soils in Slovakia. Acidification is process, where acidity of abiotic compounds has been increased. Soil reaction is a significant agrochemical property markedly affecting growing and developing of plants, and has directly effect on soil fertility, influencing the ecological conditions for plants and soil microorganisms. The site had been localised with GPS and 5 sampling places had been fixed. From these sites the soil samples were taken from 1 depth, A horizon (0-0,2 m) and then processed and managed according to particular ISO norms. The soil reaction and the heavy metals contents in solution of aqua regia and HNO₃ in soil samples were assessed. Afterwards the gained results had been compared with limit values from legislative documents.

To better understand the chemical transformation of rainfall after the passage through the canopies it is necessary to study throughfall deposition fluxes within forest stands. The comparison of bulk deposition fluxes of Ca, Mg, K, S-SO₄, N-NO₃ and N-NH₄ in mountain spruce stands of different stand density and bulk deposition fluxes on the open area was made at the study site Bílý Kříž (Moravian-Silesian Beskids Mts., Czech Republic) during the period of 1999-2006. A linear relationship between the amount of rainfall on the open area and the amount of throughfall in the spruce stand was found. Throughfall deposition fluxes of selected elements in the dense as well as in the sparse spruce stands were higher when compared with bulk deposition fluxes on the open area. There were mostly statistical significant differences between the bulk deposition fluxes on the open area and those in the studied spruce stands. The throughfall deposition fluxes of Ca, Mg, K and S-SO₄ were influenced by the spruce stand density.

The study compares two methods for modeling the potential distribution of pests when applied to the European corn borer (Ostrinia nubilalisHubner). The development of the European corn borer (ECB) is known to be closely correlated with daily air temperature as well as other climate variables. The climatic parameters are, therefore, used to predict the potential geographical distribution using tested tools such as CLIMEX or ECAMON. These models consider the climatic suitability of a given site/region for the pest's development and, thus, the possible establishment of a population at a given location. In this study, meteorological data from 1961 to 2000 and from 45 meteorological stations were used to characterise the current climate conditions in the Czech Republic. Validation was based on available field data of the occurrence of ECB in the same period. The climate parameters were later modified according to the estimates based on the combination of three SRES emission scenarios and three global circulation models. Under all climate change scenarios, we noted a marked shift of the pest's potential niches to higher altitudes, which might lead to an increase in the infestation pressure during the first half of this century. The present area of the univoltine population will increase due to temperature increases even above 800 m a.s.l. In addition there is a risk of the establishment of a bivoltine population in the main agricultural areas and 38% of arable land in the Czech Republic before 2050.

A 4-year-old, intact male crossbreed dog, weighing 27 kg, was referred for the treatment of a nonunion fracture. The radiographs revealed displacement of the radius and ulna bone fracture fragment and a sclerotic fracture end of the radius. Autologous adipose derived stem cells (ADSCs) were isolated and expanded ex vivo in a culture. The ADSCs (3.2 × 107 cells) were seeded on a composition scaffold made from hydroxyapatite (HA) and chitosan (CH) fibers. The seeded scaffold with ADSCs was placed on the fracture site and the bone fracture was stabilized. A sample of seeded scaffold with ADSCs was taken to evaluate the extent of cell attachment and morphology on the scaffold using scanning electron microscopy (SEM). SEM showed that ADSCs adhered to the scaffold well and many bone nodules formed from the bone matrix secreted by ADSCs. Three months after surgery, the nonunion had successfully healed with no complications. The application of a composition scaffold of HA and CH containing ADSCs can be used to treat a nonunion fracture by augmenting bone healing and may decrease the risk of surgical failure of nonunion fractures.

The paper suggests and examines a simplified relative indicator of forest production, with special regard to possibilities of its use in projecting future forests. Forest yield index (I_Y), based on an economic parameter "value of final cutting yield" was proposed, and examined in the model territory of Kysuce in north-western Slovakia. The current values of final cutting yield, dependent on tree species, site index and the length of rotation period served as a basis for the assessment of expected yields. The possibilities and limitations of index applicability in long-term strategic forest management decision-making are discussed, considering the uncertainty of ecological and economic conditions during the long forest production cycle, as well as the complexity of tree species growth and production in the mixed forests, uneven aged forests and forests under climate change.

The aim of our study was to determine the total mercury and methylmercury contents in the muscle of indicator fish, to determine the total mercury contents in the sediment, and to evaluate the health risks associated with fish contamination. Chub (Leuciscus cephalus L.) were caught in seven localities on the Svratka and Svitava Rivers in the Brno agglomeration in 2007. The total mercury content was determined by atomic absorption spectrophotometry using an AMA 245 analyser. Methylmercury levels were determined by gas chromatography (using electron-capture detector) after acid digestion and extraction with toluene. The highest levels of total mercury and methylmercury contamination in the fish muscle (0.18 ± 0.09 mg/kg and 0.16 ± 0.09 mg/kg, respectively) were found at the Rajhradice site (the Svratka River, under the Brno city), whereas the lowest contents of mercury and methylmercury (0.08 ± 0.02 mg/kg and 0.04 ± 0.03 mg/kg) were detected at the Modřice site (the Svratka River). Total mercury content in the sediment ranged from 0.06 mg/kg to 1.38 mg/kg, the higher value having been detected in the sediment from the Svratka River at the Rajhradice site above the confluence with the Svitava River. The lowest content was discovered at Kníničky (the Svatka River). The hazard indices calculated for the selected localities showed no health risk to the common consumer or to the fishermen and their families.