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Disturbing the remaining forest ecosystem in the Cibodas Botanical Garden (CBG) has affected the dynamics of the soil macroarthropod communities. This study was conducted in three remaining forest locations in the CBG with different levels of disturbance. Soil macroarthropod samples were collected using the pitfall trap method with 30 traps and analysed using the Shannon-Wiener diversity index, Pielou's evenness, Simpson's dominance, and Margalef's species richness to assess the dynamics of the soil macroarthropod community. This study analysed how these communities respond to different levels of disturbance in the garden, namely Jalan Akar (JA; low), Wornojiwo (WJ; moderate), and Ciismun (CI; high), which were influenced by tourism activities and local environmental conditions. The results showed that individuals from the Hymenoptera group accounted for 60.05% of the total number of soil macroarthropods found. Site WJ, which experienced moderate disturbance, had the highest number of individuals and species richness of soil macroarthropods. In contrast, site CI, which experienced high levels of disturbance, had a lower number of individuals and lower species richness, diversity and evenness indices. Site JA, which experienced low levels of disturbance, exhibited higher diversity and evenness indices. These results demonstrate that disturbance affects the presence of soil macroarthropods at their respective levels of disturbance. However, analysing the spatial distribution of soil macroarthropods in each studied taxon using the Morisita index revealed that they were dominantly clustered and exhibited varied distribution patterns. The study concludes that maintaining minimal disturbance is essential to preserve soil biodiversity and ecological balance in managed forest ecosystems such as the Cibodas Botanical Garden.

Climatic microrefugia allow some forest tree species to persist outside their main distribution range by locally decoupling site conditions from the regional climate. At its western, oceanic range margin in the Swiss Prealps, the Swiss stone pine (Pinus cembra L.) occurs on large boulders embedded within subalpine forests dominated by Norway spruce [Picea abies (L.) H. Karst.]. We hypothesised that these landforms generate a continental-like microclimate enabling P. cembra persistence under otherwise sub-oceanic conditions, and we aimed to quantify this phenomenon. Using high-resolution data loggers, we measured air and soil temperature, thermal amplitudes, and soil moisture on the summits and at the bases of ten limestone boulders over a two-year period. Linear mixed-effects models revealed a pronounced microclimatic decoupling between positions. Boulder summits were consistently warmer and drier during the growing season. In contrast, during winter, summits were significantly colder, while boulder bases remained thermally stable due to persistent snow insulation. The strongest microclimatic divergence occurred in spring, when temperatures at the bases remained stable near 0 °C, whereas summit temperatures were markedly warmer and more variable. This continental-like microclimate likely promotes P. cembra persistence. Our results highlight the importance of topographic heterogeneity for maintaining marginal tree populations.

The suitability of macadamia trees for different climates has been shown to affect tree growth and fruit quality. In this study, the differences in tree survival rate after frost injury, tree growth, nut mass, kernel quality and yield of seven macadamia cultivars were evaluated for field production in the high altitude and high latitude climate of southern Tibet, and the suitability of macadamia cultivars was determined. The cultivars evaluated were ‘Beaumont’ (‘HAES695’), ‘HVA16’ (‘A16’), ‘Hinde’ (‘H2’), ‘Own Choice’ (‘O.C.’), ‘SSCRI-1’, ‘SSCRI-2’ and ‘SSCRI-3’. Of these, ‘Beaumont’, ‘A16’, ‘H2’ and ‘O.C.’ were Australian cultivars and ‘SSCRI-1’, ‘SSCRI-2’ and ‘SSCRI-3’ were Chinese cultivars. The results showed that all seven cultivars grew well under the high altitude and high latitude climate conditions of southern Tibet without continuous frost. ‘A16’ and ‘Beaumont’ in addition to their reasonable yield, nut mass and kernel quality, had the higher tree survival rate after frost injury. ‘A16’ and ‘Beaumont’ were well adapted to the high altitude and high latitude climate of southern Tibet, followed by ‘O.C.’, ‘SSCRI-1’ and ‘SSCRI-2’, but ‘SSCRI-3’ and ‘H2’ were unsuitable. These results indicated that it is possible to produce macadamia in the high altitude and high latitude climate of southern Tibet by planting excellent frost-resistant cultivars, with promising yields and a commercial standard of nut quality.

Clear-cutting is the most common silvicultural system. Sometimes, if the new crop is not established successfully, clearcut is left unreforested. This study focused on a site where early successional species such as silver birch (Bi) and rowan (Ro) were accompanied with Norway spruce (Sp) in 13-year-old stand from natural regeneration at 550 m of altitude at an acidic site with eastern aspect and 25% slope. We found five types of stand composition: treeless gaps, Ro-Bi, Ro-Bi-Sp, Bi-Sp and monospecific Sp. Besides these juvenile ones, adjacent 100-year-old spruce (Sp old) stand representing pre-harvesting conditions was studied. In addition to the performance of trees, organic layer (Hum), topsoil (Ah) and upper subsoil (B) horizons were sampled to study an expected shift of chemical properties after clear-cutting and secondary succession at the site of interest. Birch dominated the natural regeneration; rowan and spruce were present mostly in understorey. Old spruce was more acidic and nutrient-poorer compared to the juvenile treatments. The treeless treatment showed also slightly higher pH and comparable nutrients compared to the young mixtures. Young spruce was higher in nitrogen compared to Ro-Bi-Sp mixture.

Moisture content regulation of root crops is crucial in post-harvest processing operations, not only in the price stipulation but also to avoid aflatoxin contamination. To prolong their storage life, they are processed into dried chips to extend their usability in feed formulations and starches. In this study, we use the capacitance-based method to evaluate the performance of an analogue-based electronic meter for the cassava, sweet potato, and taro chips. The meter was calibrated against the oven-drying method, yielding high R² values of the different root crops. The established calibration models were validated and revealed high R² values with 0.9580 for the cassava, 0.9958 for the sweet potato, and 0.9798 for the taro. The trendline equations are y = 59.44x^0.56, y = 54.38x^0.47, and y = 52.94x^0.62, respectively. The results revealed that the moisture meter is capable of reading the moisture content on a weight basis (% MC_wb) with accuracy and reliability at specified limits of 8% < x < 69% for the cassava, 15% < x < 59% for the sweet potato, and 9% < x < 57% for the taro. This study presents the performance of a portable analogue-based moisture meter as a reliable and accessible solution to small-scale operations, especially for farmers, offering an on-site rapid moisture content measurement in root crop processing.

Reliable indicators of early soil biological change remain limited in tropical agroecosystems, where soil organic carbon (SOC) stocks may respond more slowly than microbial processes. We evaluated whether seasonal vegetation dynamics derived from Sentinel-2 fractional vegetation cover (FVC) are associated with spatial variation in SOC stock and microbial indicators in Jembrana, Bali, Indonesia. We mapped seasonal FVC from 2019 to 2024 and derived site-level metrics of mean cover and temporal variability (standard deviation, anomaly, coefficient of variation, and a temporal stability index). In July 2023, we sampled topsoil (0–30 cm) at 12 sites representing contrasting land uses and topographic settings. We calculated SOC stock from organic carbon concentration, bulk density, and sampling depth, and measured basal respiration and culturable microbial density (colony-forming units, CFU). Vegetation cover peaked consistently during the wet season (December to February), and mean site FVC ranged from 0.31 to 0.99. Mean FVC showed positive but non-significant associations with culturable microbial density (Spearman’s ρ = 0.48, P = 0.114) and basal respiration (ρ = 0.29, P = 0.361), whereas higher vegetation variability metrics tended to coincide with lower culturable microbial density (ρ = –0.43 to –0.51, P = 0.090 to 0.163). SOC stock showed near-zero coefficients and no statistical evidence of association with vegetation metrics (ρ = 0.09, P = 0.781) or microbial indicators (ρ = 0.01, P = 0.975). Principal component analysis of FVC traits explained 99.65% of the variance and separated sites along a gradient from stable, high cover to more variable, lower cover. Overall, FVC stability metrics captured spatial differences that were directionally consistent with microbial indicators, but associations were not statistically significant in this dataset (n = 12). Larger, replicated studies with repeated soil sampling are required to evaluate whether seasonal FVC metrics have robust predictive utility for SOC stock and soil biological indicators.

This study evaluated a novel slow-release copper fertiliser (soileos Cu) as a sustainable alternative to conventional copper sources for improving wheat yield and nutrient use efficiency. Traditional Cu fertilisers are often limited by rapid leaching and low efficiency, especially on sandy soils with low organic matter, contributing to environmental pollution. They also exhibit low plant-use efficiency due to strong adsorption and immobilisation in soils rich in organic matter and clay minerals, thereby reducing copper availability in the soil solution and contributing to environmental pollution. A multi-scale approach was employed, including laboratory incubation, greenhouse experiments, and multi-site field trials. Copper release was quantified in water over 30 days. Greenhouse experiments compared soileos Cu with copper sulfate (CuSO₄) across multiple application rates, assessing grain yield, biomass, spike count, chlorophyll index (SPAD), and tissue and grain nutrient concentrations. Field trials were conducted at four sites in Canada and the United States with contrasting soil Cu availability. Soileos Cu exhibited controlled, non-linear Cu release with substantially reduced leaching compared to CuSO₄. In greenhouse conditions, soileos Cu achieved maximum grain yield, biomass, and spike number at 25–26% lower Cu application rates than CuSO₄, indicating higher nutrient use efficiency. Field trials confirmed that yield responses were strongly dependent on baseline soil Cu levels, with the greatest yield increase (up to 13.3%) observed at a Cu-deficient site. Overall, soileos Cu provides an effective and environmentally responsible strategy for improving Cu nutrition and wheat productivity, particularly under Cu-limiting conditions.

Biomass nutrient loss and retention were studied at nutrient-poor forest site dominated by Scots pine where two methods of logging residues handling after clear-cutting were compared. The experiment was conducted on nutrient-poor pine-oak forest site on deep sandy-gravel unconsolidated sediments at the altitude of 255 m. There were three treatments established such as (i) control - no harvesting, (ii) whole-tree harvesting with ca. 10% of the slash retained unintentionally on site as processing residues and (iii) stem-only harvesting when small-diameter wood and slash were left on site. The third treatment was found to retain much larger amounts of nutrients in logging residues representing 16% of total above-ground dry mass which accounted for 58% of N, 32% of P, 56% of K, 22% of Ca and 28% of Mg left on site.

Long-term human activities substantially altered floodplain regions of temperate Europe. Forest management and extensive changes in hydrology greatly affected natural floodplain soil properties, in which microbes play key roles. This study aims to assess the effects of human activities through a gradient of forest management intensity on soil microbial community (SMC), its biomass, activity, and structure. Soil chemical and physical-chemical properties were used to explain the general associations and within-site variation using principal component analysis (PCA), linear regression (LR) and linear mixed-effect regression (LMER) models. It was found that forest management application, regardless of its intensity, led to significant microbial biomass reduction. PCA revealed that microbial biomass, expressed as a sum of phospholipid fatty acids along with recalcitrant carbon fraction (ROC) best explained the variability in data. LR and LMER highlighted that bacteria are affected by floodplain forest management more than fungi, and that bacterial response to pH was highly diversified. Also, pH was identified as the best predictor of SMC structure and activity but not of its size. The study calls for further investigation in SMC interactions with ROC, soil-available Fe and Mn, and the role of redox-active metals in soil organic carbon degradation.

Forest bathing is gaining attention for its health and well-being benefits, leading to growing interest among academics, policymakers, and practitioners. While most studies have focused on its physiological and psychological effects, less is known about how different stakeholders perceive the characteristics that make forest bathing effective and meaningful. This study explored expert and non-expert perspectives on forest bathing in Italy through the Q-methodology, aiming to identify shared and divergent views on key aspects such as site features, accessibility, and perceived benefits. The results highlighted four distinct viewpoints: a scientific-health-oriented perspective emphasising medical efficacy (mainly experts); a preference for more naturalness and ecological integrity (mainly non-experts); a possible socio-economic opportunity (mainly non-experts); and a preference for a more managed forest environment with supporting structures (mainly experts). Despite differences, consensus emerged on several points, including the cultural and physical relevance of forest bathing and the importance of inclusive accessibility while preserving low-impact environments. The findings highlighted the potential of Q-methodology to capture the plurality of stakeholder voices, offering a basis for more inclusive planning and policy development in the context of nature-based well-being initiatives.

The large number of charcoal kiln sites (CHKS) preserved as cultural heritage monuments demonstrates how extensive forest management for charcoal production has influenced the present forest dynamics and contributed to industrial expansion. Therefore, it is necessary not only to have a reliable methodology for detecting and documenting individual kiln sites for management and protection purposes but also to present the results in a meaningful way to the public. The aim is to optimise the data processing workflow from airborne laser scanning (ALS) point cloud to printable model (from LAS format to STL), determine the influence of vegetation cover at the time of data collection on the quality of the resulting model, verify the quality of printed models using photogrammetry, and finally, produce printed models of CHKS as cultural heritage objects in a form that can be effectively presented to the public. After comparison of different ground filtering methods, we conclude that the most accurate method for creating a precise ground representation for our area of interest was the Cloth Simulation Filtering (CSF) algorithm. From the filtered point cloud, a high-resolution raster surface was generated, which served as the basis for CHKS detection. Using our proposed methodology – combining the topographic position index (TPI) with a 0–5% slope threshold – we achieved a significant improvement in detection performance compared to using a zero-slope threshold alone, with the F1 score increasing from 0.667 to 1.000. Subsequently, the most representative kiln site was selected, which was then processed and optimised using various software tools, resulting in an exchangeable STL file suitable for dissemination and 3D printing. The accuracy of the printable model was then evaluated. We conclude that point cloud from the winter flight campaign achieved higher accuracy. The average height differences were similar; however, the spatial distribution varied between the two clouds. The model from the winter flight campaign had more evenly distributed deviations and overall was better for modelling the CHKS.

This study investigated the potential risks of cadmium (Cd) toxicity in buffaloes grazing on forages that were cultivated in soils irrigated by various sources of wastewater. The absorption of trace metals (TM) by plants and their subsequent entry into the food chain pose a significant danger to grazing animals through the accumulation of contaminated fodder. The mean concentration of Cd in the water ranged from 0.18–1.78 mg/L, in the soil 0.63 to 1.87 mg/kg, in the forage 0.20 to 1.32 mg/kg, and in the blood 0.26 to 1.98 mg/L. Among all three sites, canal water (CW Site I), groundwater (GW Site II), and sewage water (SW Site III), the concentration factor (CF) values were below the threshold of 1 (CF < 1), indicating the nominal environmental concern regarding Cd contents in the soil-plant interface. In addition, a prominent variation was noticed in the transfer factor (TF) of Cd across different sites, with the highest TF observed in Avena sativa L. at SW Site III (0.8) and the lowest in Pennisetum glaucum L. at CW Site I (0.27). Furthermore, the hazard quotient (HQ) exhibited a substantial fluctuation, ranging from 0.39 to 2.6, reflecting varying levels of potential health risks associated with Cd exposure. The outcomes of the current investigation suggested that the prominent increase in Cd levels was recorded at sampling site SW Site III due to continuous wastewater irrigation. Prolonged exposure and increased Cd absorption in buffaloes grazing at these sites could have harmful long-term effects on their health. The correlation analysis between Cd concentrations in water, soil, forage, and blood showed a positive but non-significant relationship for water-soil, soil-forage, and forage-blood interactions. This highlights the need for further research to assess the long-term implications of wastewater irrigation on heavy metal accumulation in livestock.

Coffee leaf rust (CLR), Hemileia vastatrix, is a disease that has severely impacted the major coffee growing areas of the world. The discovery of CLR in Hawaii in October 2020 was devastating news to local growers, as it quickly spread to all coffee-growing areas, threatening the second-highest-valued crop in the State. Although the fungicide Priaxor Xemium had been approved as a Section 18 emergency exemption, there was still an urgent need for true systemic fungicides to provide sustained control of the disease. The study's overall objective was to test the efficacy of various systemic fungicides against CLR in field conditions. During the coffee growing season for two years, various fungicides were tested on two farms in the Kona district of Hawaii. Treated trees showed an 84% reduction in CLR incidence compared to untreated trees in Year 1 and a 79% reduction in Year 2 by the end of the study. Treated trees also had 12% and 38% more total leaves per branch than untreated trees in Years 1 and 2, respectively. All fungicide treatments provided excellent or good control of CLR compared to the untreated control under the climate of Hawaii.

After spruce, the Scots pine (Pinus sylvestris Linnaeus) is the second most important commercial coniferous tree species in the Czech Republic. However, we are finding out that awareness of the variability of properties, and possibilities to affect them, are noticeably small for this type of tree species in our conditions. The goal of this study is to primarily evaluate the importance of site conditions, silvicultural measures and other factors for the density of Scots pine wood in the Doksy locality in the Czech Republic. The Doksy locality is represented by three forest stands with different silvicultural history. Samples were taken from each stand, the basal and central parts of which were subsequently processed for test samples with dimensions of 20 × 20 × 30 mm. Wood density at 12% moisture content was ascertained in the test samples. The highest density value of 0.541 g.cm^-3 was reached in a stand that is regenerated using the shelterwood method with long regeneration period, and the lowest density value of 0.488 g.cm^-3 was recorded in a stand that was regenerated using the clear-cutting method. From a forestry perspective, it can be further stated that the wood density of Scots pine is also affected by the site conditions and position of samples in the trunk.

Near-Infrared (NIR) spectrometry has emerged as a promising tool for the non-destructive and rapid analysis of temperate fruit quality, maturity, and other parameters. The technique provides a wealth of information, including details of chemical composition, without damaging the fruit, making it a highly viable alternative to traditional methods. This paper reviews the recent research and applications of NIR spectrometry for fruit evaluation, highlighting its strengths and potential limitations. The analysis shows a significant potential for NIR spectrometry, especially when combined with machine learning and artificial intelligence to handle complex data and improve predictive models. The development of portable NIR spectrometers allows for in-situ quality assessment, expanding its applicability to various fields including on-site quality control. Despite the benefits, this review identifies key challenges including spectral complexity, fruit variability, and the influence of the external environment. Recommendations for future research include focusing on improving calibration and validation of models, increasing predictive accuracy, and developing user-friendly instruments. In addition, standardization of measurement procedures and analytical methods is needed to ensure comparability and reproducibility of results. Further research is needed to fully realize the full potential of NIR spectrometry in fruit quality control.

The study focused on the relationship between leaf-to-fruit ratio (LFR) and walnut kernel quality in Juglans regia ‘Wen 185’. It was investigated how LFR influences single kernel weight, the contents and percentages of organic matter in kernels, the 13C distribution proportion (¹³C_DP) in crude fat and protein, and the number and size of oil bodies within the kernels. A gradually decreasing LFR led to reduced single kernel weight, the contents of crude fat, crude protein, and soluble sugar dramatically (P < 0.05), with no significant changes in the percentages and (¹³C_DP) of crude fat, crude protein, and soluble sugar (P > 0.05). Moreover, there were no significant differences in the number of oil bodies per unit area and the size of oil bodies in kernel cotyledons and endosperm storage cells among the different LFR (P > 0.05). We propose that the walnut kernel quality depends on the proportion of sugar converted into fat and protein in the kernels, that the changes in LFR affect the amount of sugar accumulated in kernels but not the proportion of sugar converted to fat and protein, and that the LFR, therefore, have no effect on the percentages of crude fat and crude protein in walnut kernels.

The planting of branched nursery trees is an essential component of most high-density orchard planting systems to induce precocity. However, in apples, most cultivars do not produce the desired numbers of sylleptic shoots naturally due to the presence of apical dominance. Benzyladenine (BA) applications alter the apical dominance and consequently encourage sylleptic shoot formation in nursery trees. However, the response to an exogenous BA application can vary with the genotype. Currently, most nurseries produce branched apple nursery trees using the renewal leader method. However, apart from the renewal leader method, branched nursery trees can also be produced using the central leader method. A comparative study of these two methods had not been conducted previously, and this investigation aimed to determine the effects of both the central and renewal leader methods, as well as the genotype, on sylleptic branching in apple nursery trees in response to repeated BA sprays. The genotype showed significant variation in the sylleptic shoot numbers (5.25–9.41), their average length (26.86–33.34 cm), and crotch angle (48.95˚–54.27˚) in response to the BA application. Among the genotypes, ‘Shireen’ produced the highest number of sylleptic shoots, whereas the opposite results were obtained in ‘Top Red’. Furthermore, irrespective of the genotype, the central leader method was found to be more effective than the renewal leader for the development of high-quality branched nursery trees. The central leader method not only significantly increased the number of sylleptic shoots on the tree but also positively affected their length, final tree height, and diameter.

This study aims to compare the soil quality indexing model by adding and weighting the soil under different land uses and slope positions on the southwest slope of Mount Merapi, Indonesia. Soil sampling was carried out based on a landscape analysis divided into four geomorphological units (slopes): upper, middle, lower and foot slopes. The research design was nested where the soil sample was located (surface soil 0–30 cm). Based on the research results, soil quality indices (SQI) of forest on the upper slopes is very high. SQI of dry fields on the middle, lower and foot slopes is low to medium. SQI of mixed gardens on the middle and lower slopes is low to medium. SQI of snake fruit land on the middle, lower and foot slopes is medium to high. SQI of grassland on the lower slopes is medium to high, and SQI of paddy fields on the foot slopes is medium to high. Weighted soil quality index (SQIw) has a higher correlation (R² = 0.90) and can predict soil quality better than the adding soil quality index (SQIa) model (R² = 0.76). Indicators that most influence soil quality are the percentage of sand, total N, C-POM, C-Min, pH, and aggregate stability, that indicators are entirely influenced by organic matter, site-specific management to maintain SQI by maintaining organic matter. The selected indicators in this study can be used to determine the SQI in similar areas.

Resveratrol and polydatin are stilbenes with notable antioxidant and health benefits, making them important compounds in health promotion, grapevine production, and oenology. This study aimed to monitor the trans isomers of these compounds in twelve grape varieties (red and white), grown under organic production (OP) and conventional production (CP) systems. A novel high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) method was developed and validated, demonstrating sensitivity and reliability for the quantification of the mentioned stilbenes. The method was applied to real samples, enabling the evaluation of the influence of production systems on the presence of the analysed compounds. The research included some of the most widely cultivated domestic and international varieties in the examined region. The obtained results revealed a significant impact of the production system and grape colour on resveratrol and polydatin content. OP consistently yielded a higher content of both compounds, compared to CP. Furthermore, red varieties showed higher overall stilbene levels than white ones. ‘Shiraz’ variety was an exception, with high levels of both compounds under CP. All the obtained findings highlight the role of the production system in enhancing bioactive compound content in grapes. The study provides valuable insights for optimising viticulture, supporting plant resilience, and improving the nutritional qualities of grape-derived products.

This study investigated the influence of site conditions on the physical and morphological properties of hawthorn (Crataegus azarolus L.) wood grown in Zagros forests of Iran. Hawthorn is a valuable woody species in Iran's Zagros forests with data deficiency in its wood properties. To our best knowledge, there is no study on the properties of hawthorn wood. Twenty-seven mature trees were randomly chosen and sampled at three altitude levels (1 800-2 000, 2 000-2 200, and 2 200-2 400 m) in the Bazoft region of Chaharmahal Va Bakhtiari, Iran. Dry wood density, volumetric shrinkage, fibre length, fibre diameter, cell wall thickness were then determined. Multivariate analysis of variance (MANOVA) was conducted to evaluate significant differences between the mean values of studied wood properties at different altitude levels and slope classes. Moreover, the relationships between the above-mentioned properties and some site conditions (temperature, precipitation, altitude, and slope) as well as growth parameters of trees (tree height, DBH, age, crown dimensions, volume, etc.) were studied by principal component analysis (PCA). The results indicated that trees growing at the second altitude level had the highest oven-dry density values, and those at the third altitude level had the lowest ones. Results also indicated a significant effect of altitude levels on fibre length, fibre diameter, and cell wall thickness. In contrast, a significant difference was found between the mean values of fibre diameter at different slope classes. PCA indicated that altitude, precipitation, and temperature are the most important environmental site variables affecting the wood characteristics.

Missouri Ozark woodlands are a unique, but imperilled ecosystem type due to fragmentation, lack of proper management and a changing climate. The management, restoration, and conservation of Ozark woodlands is a conservation priority. The Ozark woodlands contribute to the sequestration of carbon and nutrients through their robust productivity, effectively removing carbon dioxide from the atmosphere and storing it in the biomass and soil while cycling essential nutrients to support the ecosystem's health and vitality. We have assessed the over- and mid-storey leaf production, collecting leaves in baskets every autumn in a 1 200-ha conservation area in southwest Missouri since 2000. The leaf production data from 2000 to 2021 were compared among sites; control (not burnt for over 80 years), burnt (fire resumed in March of 1999 and repeated in 2001, 2003, 2008, 2010, 2013, and 2021) and reference (fire resumed in 1980 and repeated every 2 years). The average oak leaf production was statistically higher in the burnt site than the reference site, but only marginally higher than the control site. The leaf production varies statistically between the years. We applied a regression analysis among the productivity, temperature, and precipitation to associate the temporal variability in the weather with the productivity. The reference woodland showed statistical significance with the precipitation, but not with the temperature, while the other sites did not show any statistical significance with the precipitation. No statistically significance difference was observed between the productivity and temperature across any of the woodland burn histories. The March–June, March–May, and June–August precipitation statistically predicted the productivity. The results indicate that long-term burning is predictably associated with woodland leaf production and precipitation, but the precipitation is uncoupled with the productivity in woodlands that were more recently burnt or where burning has been suppressed. Sassafras saplings of approximately 1 m in height have emerged as the dominant species in the understorey of burnt woodlands while being completely absent from the control and reference woodlands. However, the productivity is the highest in the woodlands where burning has been suppressed and 20 years of prescribed fire does not significantly reduce the productivity. Oak regeneration over 20 years of burning is being suppressed by competition with sassafras, which may result in a significant shift in the ecosystem variables.

Ground heat flux (G) is often an overlooked component of the surface energy balance, and its accurate determination remains challenging. In the present study, the accuracy of various G estimation methods is examined using long-term measurements from the Central European mountain meadow. The impact of different G approximation on calculated evapotranspiration by the Penman-Monteith method (ET) is analysed. Soil heat flux measurements and surface temperature data were used to determine G, while net radiation was used to approximate G. Neglecting G led to an overestimation of ET in the daily timestep. On the contrary, the FAO-recommended hourly approximation overestimated G, underestimating ET. Site-specific calibrations of G prediction models improved their accuracy. For daily average G, as well as for hourly average G, simple constant parameter models (i.e., models including a single parameter specifying the fraction of net radiation directly) provide satisfactory accuracy of ET evaluation. However, in an hourly timestep, net radiation fails as a predictor of G shortly after sunrise and before sunset. The findings emphasise the importance of considering G in ET calculations and the need for site-specific calibrations of G estimation models.

In recent years, grafting has emerged as an efficient and alternative tool to the relatively slow conventional breeding methods, aiming to increase tolerance to abiotic stresses and soil pathogens while improving yield and quality attributes in fruit vegetables. In the present investigation, six inter-specific cucurbitaceous rootstocks, viz. sponge gourd (SG), ridge gourd (RG), ash gourd (AG), bottle gourd (BG), and Summerfit (SF), an inter-specific hybrid of snap melon × acidulus melon, were evaluated for cucumber (C) cv. ‘Kashi Nutan’. Experimental findings revealed that cucumber grafted onto inter-specific SF exhibited a 14.63%, 57.5%, and 20.05% increase in vine length, number of branches, and dry matter production, respectively, compared to the self-rooted control. Photosynthetic parameters such as photosynthetic rate (P_n), stomatal conductance (g_s), and maximum quantum efficiency of photosystem II (PS II) (Fv/Fm) were also higher in cucumber leaves grafted onto the SF rootstock. Compared to self-rooted plants, cucumber grafted onto SF recorded 72.3% more fruits, a 36.9% increase in fruit weight, and an 80.9% higher fruit yield. The SF rootstock also showed a 44.54% increase in total root length (TRL), a 77.11% increase in root volume, and a 27.25% increase in average root diameter over self-rooted cucumber.

The ecological significance of Scots pine (Pinus sylvestris L.) in Europe, especially in areas devastated by bark beetles, has led to its consideration as a substitute for Norway spruce. This pioneering species boasts sun and drought tolerance, fast growth, and wood industry value. To gauge its potential, we examined two ecotypes across two test sites over two years, focusing on height and growth increment. Through statistical analysis employing R software and linear mixed models, we assessed heritability, genotype by environment interaction, and spatial correlations. Both ecotypes exhibited significant differences in height and increment, varying by year and site. Heritability was higher in the second year, with increment showing greater stability. Genetic correlations between sites were evident, suggesting stable increment ranking across locations. These findings underscore the role of ecotypic variation in Scots pine growth, advocating for its consideration in reforestation. Acknowledging such dynamics is vital for effective forest management and reforestation in Central Europe, promoting sustainability and informed decision-making. Further research will enhance this understanding of preserving and enhancing the region's tree populations.

The apple scab, caused by Venturia inaequalis, is one of the most devastating diseases of apples because it drastically affects the aerial parts of the tree. Apple breeding programs frequently aim to develop cultivars exhibiting resistance to scab. Thus, controlled pollinations were performed on apple trees in 2021. The experiment was organised into a hybridisation scheme involving six apple cultivars. The scheme consists of: five female genitors (‘Auriu de Bistrița’, ‘Jonathan’, ‘Golden Delicious’, ‘Starkrimson’, and ‘Idared’) and one male genitor (‘Florina’ – donor for the Vf). To quickly identify the Vf gene in the early development stage of progenies, three pairs of primers were used: AM19, AL07, and VFC. The hybrid combinations with the highest percentage of progenies inheriting the Vf resistance gene were: ‘Starkrimson’ × ‘Florina’ (59.2%), followed by ‘Jonathan’ × ‘Florina’ (57.1%). The hybrids that inherited the Vf resistance gene have been transferred to the field and are being further examined for their resistance against scab infections in the field, in the proximity of a natural source of the inoculum infection pressure.

The aim of this study was to analyse the effects of harvest timing on the total phenolic and flavonoid contents, in addition to the total antioxidant capacity in the berries of ‘Žilavka’ and ‘Vranac’ varieties grown in the Trebinje vineyard area. Grape samples for both varieties were collected at three distinct points during their ripening. The chosen harvest dates were set 10 days apart, and the optimal date for grape harvesting was determined by assessing the sugar content and titratable acidity present in the grapes. Total phenolics, total flavonoids and total antioxidant activity of grape samples were determined by the Folin-Ciocalteu method, aluminium chloride method, and ferric reducing antioxidant power assay, respectively. The total phenolic and flavonoid contents, along with the total antioxidant capacity in the ‘Vranac’ grape, increased from the first to the second harvest date, but a decline was noted at the third harvest. ‘Žilavka’ grape exhibited an increase in total phenolic and flavonoid contents, as well as total antioxidant capacity from the first to the second harvest date, with no decrease recorded by the third harvest. As expected, the red grape variety ‘Vranac’ contained higher levels of phenolics and flavonoids than the light-skinned grape variety ‘Žilavka’.

There is significant interest in the propagation of bananas due to their substantial global economic importance, which is why this study aimed to develop an efficient protocol for in vitro propagation through somatic embryogenesis (SE) of the ‘Manzano’ and ‘Pelipita’ cultivars. Immature male flower bud explants were used for embryogenic callus induction. The ‘Manzano’ cultivar showed a higher percentage of embryogenic callus induction than ‘Pelipita’. Stereomicroscopic observations revealed that the embryogenic callus was asynchronous, as various stages of somatic embryos were noted. White translucent pro-embryogenic callus was efficiently utilized to establish embryogenic cell suspension. The development of different stages in the regeneration process of embryogenic cell suspension was also recorded. Embryos in the late coleoptilar stage, characterized by an opaque white morphology, were selected and transferred to an appropriate medium to evaluate the germination percentage. We found that the germination rate was highly efficient for both cultivars, exceeding 90% when using these embryos in the selected stage. Additionally, we observed that embryos that were 90 days old showed a 10–12% increase in germination compared to those that were 60 days old, confirming the importance of embryo maturation duration and the selection of embryogenic aggregates through the sieving of suspended cells to optimize regeneration efficiency.

The natural regeneration of the sessile oak [Quercus petraea (Matt.) Liebl.] is an important aspect of sustainable forest management, especially given the ongoing global climate change and the need to maintain forest stand stability and productivity. This study aimed to evaluate the effect of various regeneration methods on the growth of naturally regenerated sessile oak in the Masaryk Forest Training Enterprise Křtiny, Czech Republic. The research was conducted in seven forest stands where regeneration felling was applied after masting in 2022 (winter 2022/2023) using clear-cut (CC) and shelterwood (S) systems. A total of 531 seedlings were collected from these seven research plots, and the following morphological traits were measured: shoot length, root collar diameter, and biomass allocation. Statistical analysis revealed significant differences (P < 0.05) in shoot length, main root length and total seedling length between the CC and S variants. However, there were no significant differences in root collar diameter or any of the dry-mass parameters between the clear-cut with standards (CC1) variant and the shelterwood plots. Individuals from the CC variants have a 10.3–47.0% wider root collar diameter, 22.3–91.4% more dry mass of the root system, and 51.7–90.4% more dry mass of the aboveground part than individuals in the S variants. These results indicate that the early growth of sessile oak seedlings is greatly influenced by light availability: full sunlight stimulates the development of above- and below-ground parts, whereas canopy cover restricts early growth, particularly in terms of height. The findings highlight the need to consider light and site conditions when planning silvicultural treatments aimed at establishing stable and vigorous oak stands in the face of changing climate conditions.

Recently, protected cultivation of bananas under subtropical conditions has gained popularity due to the impact of global climate change. Several factors influence the yield and quality of banana cultivation. Among these factors, variety plays an important role along with cultural practices. This study aimed to determine the performance of ‘Azman’, ‘Bango’, ‘Bonus’, ‘Grand Nain’, ‘Lider’, and ‘Paşa’ banana varieties in terms of morphological and yield characteristics under protected cultivation, as well as to elucidate the relationship between yield and factors affecting yield. The research was conducted between 2020 and 2022 in the Manavgat district of Antalya. The research findings showed that each morphological parameter examined varied significantly. However, the ‘Bango’ variety exhibited superior results in terms of finger circumference (13.23 cm), finger length (21.08 cm), bunch weight (39.33 kg), and yield per hectare (72 t/ha). Moderate to high-level phenotypic correlations were found between bunch weight and pseudo stem girth, pseudo stem height, number of fingers, finger weight, finger circumference, and finger length, with values of 0.544, 0.478, 0.326, 0.669, 0.581, and 0.543, respectively. Positive and significant phenotypic correlations were determined between finger length affecting export quality, bunch weight, finger weight, and finger circumference, with values of 0.543, 0.799, and 0.625, respectively. It can be concluded that the ‘Bango’ variety exhibited better results as compared to other varieties in terms of both yield and quality criteria. Additionally, positive phenotypic correlations were observed between bunch weight (which directly influences yield) and finger length (which affects export quality), as well as various morphological and pomological characteristics.

In Central Europe, the most important pioneer species are silver birch and European aspen. Changes in disturbance regime and an economic interest for this species have led to studies on this species and stands. Two naturally regenerated dense stands of birch (Betula pendula Roth – silver birch monoculture) and aspen (Populus tremula L. – European aspen monoculture) were selected from a Querceto – Fagetum mesotrophicum site to observe responses under the same conditions in Central Europe. Both stands regenerated after the allochthonous Norway spruce stands dieback at the site in 1999. Within a 10 m × 25 m transect established in both stands, the diameter at breast height (DBH) of all the trees was measured between 2015 and 2020. In addition, the height and position were recorded for all trees, and sample trees of both species were felled for biomass measurement. A higher volume production of aspen at the beginning (107.48/96.80 m³) and at the end of the experiment (178.32/143.08 m³) was accompanied with a lower above-ground wood biomass (WAB). The WAB of birch increased from 81.9 t·ha^–1 to 103.3 t·ha^–1 and aspen allocated 79.5 t·ha^–1 to 94.8 t·ha^–1 of biomass. The current annual increment of biomass for these stands was 4.3 t·ha^–1 and 3.1 t·ha^–1 in the age range of 17 to 22 years. The culmination of the volume increment has not yet occurred in any of the stands, but the mean annual increment of wood biomass has already been reached for both stands. Furthermore, the aspen stand tended to be more dynamic in terms of biomass allocation and mortality. Also, the lower self-tolerance of aspen confirmed our hypothesis: the two native pioneer species differ in their social behaviour within monospecific stands.