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Soil salinity is a growing problem for agricultural production in irrigated areas of arid and semi-arid regions. The extent of salinity levels has not been fully studied in the Kesem irrigation scheme in Ethiopia's Afar region. The objective of the study was to identify the main issues related to soil salinity and their variations, and to assess the influence of environmental variables on soil salinity using multivariate analysis (MVA). The dominant cations in the soil were found to be soluble Na⁺, Ca²⁺ and K+ while SO₄^2-and Cl^- were the dominant anions. These ions are responsible for the salinity in the scheme. Groundwater table surveys showed that cultivated fields experienced greater fluctuations in groundwater levels compared to abandoned land due to frequent irrigation. The first two principal components (PCA) explained approximately 60% and 63% of the total variation in salinity for the top and bottom layers, respectively. The difference between the top and bottom layers suggests a management influence. According to redundancy analysis (RDA), the groundwater depth and length of irrigation years were identified as the major environmental factors contributing to 99% and 52% of the variability in salinity, respectively. These findings highlight the importance of considering the rising groundwater levels in future land management decisions.

The aim of this trial was to verify the influence of various autumn-applied nitrogen fertilisers on the growth, yield and quality of winter oilseed rape. In the three years, small-plot field trials were carried out at the Research Station Červený Újezd (50.0697044N, 14.1659086E). The hybrid cultivar DK Exstorm was chosen, with a sowing rate of 50 seeds/m2. Five fertilisation regimes were tested: (1) nitrogen-free control; (2) CAN (calcium ammonium nitrate); (3) ANU (ammonium nitrate urea); (4) U (urea), and (5) US (urea with N-(n-butyl)thiophosphoric acid triamide (NBPT) inhibitor). A uniform dose of 40 kg N/ha was applied at the end of October. Fertilisers U (leaf length, root collar diameter, leaf and root dry weight) and US (number of leaves and root length) had the best growth outcomes. The highest seed yields were obtained with US (5.83 t/ha) and ANU (5.82 t/ha) applications, which outperformed the unfertilised control by 0.65 and 0.64 t/ha, respectively. CAN fertiliser appears to be unsuitable for autumn fertilisation in terms of yield. There were no statistically significant differences in oil content (%) or thousand seed weight (g) between the treatments in any of the experimental years.

The genus Alternaria has a global distribution and consists of a diverse group of pathogens. Plant-pathogenic Alternaria spp. can reduce the crop yield and pose serious threats to agricultural production. The pathogen A. mali is recognised as the key the pathogenic mechanism in the early defoliation of apples, which produces the host specific toxin (HST) that was named as an apple specific toxin (a specialised toxin of A. alternata pv. mali, AM toxin). The phenomenon of horizontal transfer of the AM toxin gene from different strains of A. alternata was found, and the relationship between the AM toxin and pathogenicity was confirmed. The representative strain A. tuberculata with the AM toxin gene was co-cultured with sixteen Alternaria strains without the AM toxin gene. As a result, four strains from different Alternaria species obtained the AM toxin gene, which indicated that the AM toxin gene can transfer among different Alternaria species. The AM toxin gene is easy to be lost after subculture, and high temperature and low nutrition can promote this loss. The symptoms of the Alternaria pathogen with or without the AM toxin gene are obviously different on the host. When infected by a pathogen with the AM toxin gene, green spots formed on the apple leaves, and rotten disease spots appeared in the fruit carpels. Contrary to this, when infected by a pathogen without this gene, only small epidermal spots without chlorosis formed on the apple leaves, and mildew-heart spots appeared in the fruit carpels.

The aim of this paper is to investigate whether the more pro-environmental attitudes of farmers influence the technical efficiency of agricultural sectors in EU regions (NUTS-2). To answer this, I employed data envelopment analysis (DEA) for efficiency analysis combined with the double bootstrapped truncated regression to investigate the relationship between environmental attitudes and technical efficiency. I found that this relationship is positive, i.e. pro-environmental attitudes were related to greater efficiency. An increase in the environmental attitude variable by one standard deviation led to an improvement in efficiency of 2.8–6 p.p. The higher share of farmers with formal training also proved to be a positive and significant determinant of efficiency. The share of arable land on which conventional tillage was used, and soil erosion proved to be significant but negative determinants of efficiency. Policymakers should present to farmers the environmental benefits of agricultural policy but should also highlight that greater environmental awareness translates into a more efficient operation.

Phytophthora blight of pepper, caused by Phytophthora capsici Leonian, is a destructive disease in pepper production. Extracts of the plant species Stellera chamaejasme Linn, previously known in China as a source of herbal medicine, were also used in former years as a toxin against aphids, mites, and plant-pathogenic fungi. Extracts of S. chamaejasme (SC) and seven commercial fungicides were investigated for their inhibition of mycelial growth and germination of cysts of the pathogen in vitro. The SC alone, infinito (fluopicolide + propamocab + hydrochloride), fluazinam, dimethomorph, and their mixtures with SC strongly inhibited both mycelial growth and germination of cysts of P. capsici. Tests were conducted in a commercial field in Fenggang County, Zunyi City, Guizhou Province, China, in 2019, 2020, 2021, and 2022 to validate these results for growers. A soil application (7 days before transplanting) and three foliar sprays at 7, 10, or 15 days were tested. One soil application of SC followed by three successive foliar sprays of infinito, fluazinam, and dimethomorph at 7- or 10-day intervals, as well as tank-mixed applications of these synthetic fungicides with SC at 15-day intervals, limited Phytophthora blight incidence to < 10% on the non-treated control treatment. Disease incidence was limited to < 3% to < 5% when alternating SC, SC + infinito, SC + fluazinam, and SC + dimethomorph three times at 7-, 10- or 15-day intervals, plus one soil application of SC. When treatments were applied from early April to May, disease incidence was < 10% in the non-treated control during June and July. The optimal concentration of SC was determined to be 0.25 g/L, which effectively controlled Phytophthora blight and protected marketable yield. SC also significantly (P < 0.05) outperformed treatments in a nearby commercial pepper field that relied on applications of a single synthetic chemical fungicide. The findings of this study provide a foundation for guiding growers to implement an efficient and environmentally safe spray program against Phytophthora blight of pepper in Guizhou Province.

This work aimed to evaluate the influence of soil characteristics and the applied amount of nicosulfuron on the degradation rate in soil. Soil samples were collected at three localities in Bosnia and Herzegovina – Manjača, Kosjerovo and Tunjice. The experiment was carried out under controlled laboratory conditions. Plant protection product based on nicosulfuron (40 g a.s./L, OD) was applied in concentrations of 0.075, 0.15, and 0.30 mg a.s./kg of soil. Nicosulfuron residues were analysed by the modified QuEChERS method, followed by LC-MS/MS. Soils are classified as silty loams, with variations in mechanical composition and chemical properties. In slightly alkaline soil, the half-life (DT50) of nicosulfuron has increased (43.31 days) compared with DT50 (9.43–16.13 days) in acidic soils. The results indicate that soil characteristics and applied concentration significantly influence nicosulfuron persistence. Hence, it can be considered that nicosulfuron, applied to silty loam soils of Bosnia and Herzegovina, poses a low risk to subsequent crops and the environment.

To investigate the role of the HvnAnt2 gene in the formation of different qingke barley grain colours, HvnAnt2 was isolated from the leaves of the White 91-97-3 (white), Blue qingke (blue), Kunlun 17 (black), and Purple qingke (purple). The HvnAnt2 gene encodes a hydrophilic unstable protein consisting of 561 amino acids, without a transmembrane structure and without a signal peptide, with one bHLH-MYC_N and one HLH domain at amino acids 22–204 and 387–436, respectively. The HvnAnt2 of qingke barley was most closely related to Hordeum vulgare and most distantly related to Triticum monococcum; it was mainly related to light responsiveness and methyl jasmonate responsiveness. The Ant2 gene was highly expressed in Kunlun 17 and Purple qingke barley grains during the later stage of development. The protein interaction prediction showed that flavonoid 3’-monooxygenase, anthocyanin biosynthesis gene regulators, and key enzymes in folate metabolism interacted with the Ant2 protein. This study provides a reference for further analysis of the Ant2 gene in the anthocyanin synthesis pathways of qingke barley with different grain colours.

This research investigates the high prevalence of morphological defects in the sperm of a particular rabbit breed, namely the Zemplin rabbit (ZR). We tried to uncover the possible causes and consequences of the observed abnormalities. Ejaculate samples from ten adult males were divided into two qualitative groups according to the motility parameters revealed by CASA (computer-assisted sperm analysis). Group A contained samples with total motility (TM) above 50% and progressive motility (PM) above 30%. Group B contained samples with TM and PM below these values. Viability (SYBR-14/DRAQ5^TM and SYTOX^TM Green/DRAQ5^TM staining) and apoptosis (YO-PRO ^TM-1/DRAQ5 ^TM stainig) analyses through flow cytometry served as an additional insight into the characteristics of the qualitative groups. In both cases, we noted significant differences (P < 0.05) between groups which corresponded to motility values. Both groups showed an increased incidence of abnormal spermatozoa, and we did not observe any significant differences between them. Simultaneously, we assessed the practical implications of these sperm defects on artificial insemination (AI), and also, we did not notice any significant differences in these parameters. The use of transmission electron microscopy enhanced our understanding of the morphological defects at the ultrastructural level. In summary, the study provides an understanding of the qualitative differences in rabbit semen, highlighting variations in quality parameters. While these differences exist, they may not necessarily translate into significant variations in AI outcomes. Our study shows higher values of cytoplasmic droplets in ZR semen, but it also shows that these conditions do not necessarily mean a fundamental obstacle to fertility in the case of AI.

The development of intelligent systems for the tracking of free-range livestock is a challenge to both information and communication technology (ICT) scientists and those in the animal sciences. Cyber-physical systems make it possible to track and control processes involving intelligent objects from the physical and virtual worlds. In the case of free-range grazing, it is necessary to manage processes in two domains ‒ that of the intelligent pasture management and that of the animals. Due to the differences in the conditions of different types of pastures – plain or high land and the characteristics of the cattle breeds, ready-made models cannot be used, but it is necessary to build a specific multi-aspect model for the behaviour and life cycle of cows. Our team organised their research on cows from two different breeds (Rhodope Shorthorn Cattle and Bulgarian Rhodope Cattle) raised in similar technologies, grazed on two different types of pasture. The aim of the study is to develop a comprehensive model for determining cattle behavioural activities on pastures using sensor groups, by incorporating physical observations and appropriate statistical models.

The dominant agricultural sector in Slovakia is crop production, with the majority of arable land dedicated to cultivating cereals (57%), followed by fodder crops (20%) and industrial crops (19%). Slovakia has technical and biophysical potential for expanding biomass production. However, it is crucial to identify optimal production practices, alternative costs, and environmental outputs. Farms become more vulnerable to the worldwide threat posed by climate change. Based on research, farmers can effectively mitigate the adverse effects of climate change by making necessary adjustments to their current farming techniques. Thus, by using an advanced tool like integrated farm models, farmers can evaluate and manage a range of risks related to their activities. This paper aims to present the application of integrated modelling frameworks at the farm level and propose a framework for studying the consequences of climate change through a scenario-based approach at the farm level. Integrated assessments provide new insights that complement those derived from more detailed assessments. Based on the model developed, the results of two research questions are provided. Since, from the viewpoint of the farmer, risk and unpredictability associated with lower yields are frequently the main causes of lower income, spatially explicit integrated modelling is applied, enabling economic optimisation of crop production on a selected farm with a focus on maximising net returns while considering biophysical parameters. The modelling results depict the distribution of selected crops on arable land and the most suitable management practices for crop production in terms of nitrogen application and irrigation utilisation. Additionally, we develop an integrated model proposing the estimation of the risk of yield variability and nitrogen emissions for three climate change scenarios for the simulated period of 2020–2100 on the model farm. In response to the problems posed by climate change, this integrated approach can assist evidence-based decision-making and sustainable agriculture practices.

Innate immunity is the organism’s first line of defence, and the receptors of immune cells play a key role. Interleukin 1 receptor-associated kinase 1 and 4 (IRAK1 and IRAK4) are necessary for signal transmission from Toll-like receptors, which are the most important receptors of immune cells. The main objective of this study was to investigate the gene polymorphisms of IRAK1 and IRAK4 in Czech Fleckvieh cattle (CF) and to compare their diversity with other cattle breeds as there is still little information about these genes. PacBio Technology of next-generation sequencing (NGS) was used to detect genetic variability in 164 CF bulls. The results revealed 17 single nucleotide variants (SNVs) in the IRAK1 gene and 12 SNVs and three deletions in the IRAK4 gene. Three methods were then used to determine allelic variability: Sanger sequencing, the primer extension method and Illumina DNA BeadChip microarray in cooperation with the Czech Moravian Breeder’s Corporation. Sanger sequencing enabled analysis of the population variability in single nucleotide polymorphisms (SNPs) rs210710958 and rs48726521 of IRAK1 in 164 CF bulls. SNaPshot genotyping was employed to determine the allelic frequencies of SNP rs211379365 and a novel polymorphism in IRAK1, and rs380202447 in the IRAK4 gene. DNA microarray method, which was applied to 74 CF dairy cows, revealed the allelic frequency of IRAK1 rs110533802 including the frequency 0.07 of mutant allele T. Additional microarray data allowed the comparison of the allelic frequencies among the Czech and Hungarian populations of Holstein dairy cows and beef breed populations. The results show that the functionally significant diversity in the IRAK1 and IRAK4 genes in the studied CF population is rather limited. The higher frequency 0.4 of the T mutant allele of SNP rs110533802 of IRAK1 in Holstein cattle could be the consequence of breeding for milk performance.

In this study, a synergist made of itaconic acid, maleic acid, acrylic acid and other active ingredients polymerised was sprayed on the surface of nitrogen (N) fertiliser particles to make synergistic nitrogen fertilisers (SNF). To explore the effect of SNF on N metabolism of wheat in saline-alkaline land, five treatments were set up: CK – ordinary N fertiliser (299.86 kg N/ha); T1 – SNF (299.86 kg N/ha); T2 – SNF (239.89 kg N/ha); T3 – SNF (179.92 kg N/ha); T4 – SNF (119.94 kg N/ha). The aboveground dry weight of wheat, the photosynthetic characteristics of wheat flag leaves, the activity of the N metabolism enzyme of wheat flag leaves, the expression of N transporter-related genes in wheat roots, and the N accumulation and transport of plants were determined. The results showed that the T1 treatment performed the best. During the two years, the N translocation from stems and leaves to spikes of plants at maturity in T1 was 33.18–45.55% higher than that of CK. The N content of wheat spikes was 12.01–12.66% higher than that of CK. The activities of nitrate reductase, glutamine synthetase, glutamate synthetase and the expression of nitrate transporter gene TaNRT1.1 and ammonium transporter gene TaAMT1.1 were significantly higher than that of CK. The aboveground dry weight of wheat and photosynthetic characteristics of flag leaves were significantly higher than those of CK in T1, whereas the intercellular CO2 concentration was significantly lower than that of CK. The application of SNF positively affected N accumulation and transport in wheat, wheat yield, and fertiliser utilisation, as well as reduced N loss in saline-alkaline land.

Copper contamination stemming from copper-based pesticides poses a grave concern in vineyards and orchards, causing toxicity to soil organisms. Here, we present a comprehensive review of global data encompassing copper levels in these soils, coupled with variables such as the age of agricultural establishments, climate, soil organic matter content, soil pH, and farming practices (organic vs. conventional). The results suggest that there are three pivotal determinants driving copper content in vineyard and orchard soils: climate, the age of agricultural establishments, and soil organic matter content. It was impossible to estimate soil pH’s effect on soil copper content because of its dependence on precipitation. Copper content in vineyard and orchard soils worldwide follows a direct correlation with precipitation while inversely correlating with aridity (i.e. potential evapotranspiration divided by precipitation). Furthermore, a clear linkage emerges between farm age and increased copper content in soils globally. Intriguingly, the increased soil organic matter content has shown inverse impacts on soil copper levels. These effects of soil properties on soil copper contents were discussed in terms of copper losses from soil via surface runoff. However, no discernible disparities in soil copper content between organic and conventional farming systems were found. This worldwide survey not only underscores the established influence of climate on European vineyards but also sheds novel light on the historical legacy of copper contamination in these landscapes.

The high plasticity, or the ability to differentiate into various cell types, and capacity to become both gametes of early-stage germ cells (eGCs) allows them to be used for the long-term storage and recovery of genetic information. In this study, species-specific protocols (isolation period, enzymatic separation solution, incubation period, appropriate cryoprotectant, and cryopreservation protocol) were established for the isolation and long-term preservation of Black Sea trout eGCs. In addition, the difference between the application of cryopreservation to the whole gonad tissue and to the enzymatically separated cells has been shown in terms of viability and the number of cells obtained. According to the cell count made from the histological sections of the testicular tissue throughout the year, the period with the highest concentration of eGCs in the tissue is between May and June. To identify the optimal enzymatic dissociation solution, we subjected testicular tissues to digestion using various combinations of trypsin (T) and collagenase (C) (0.3–0.5% T and 0.1–0.3% C). Our findings revealed that the 0.3% C provided the highest yield of viable cells (90.9%). We cryopreserved the enzymatically dissociated cells (EDCs) using six different cryoprotectants (Dimethyl sulfoxide, Glycerol, and CryoSOfree™, in both L-15 and phosphate-buffered saline – PBS mediums). Additionally, whole tissue exclusively with CryoSOfree™. After storing the samples at –152 °C for two years, the group of EDCs cryopreserved in L15+Glycerol exhibited the highest viability at 93.3%. Meanwhile, the whole tissue group yielded the greatest number of cells per ml after thawing, with a count of 33 210 000 cells. When considering both the viability (91.11%) and the number of cells obtained (33 210 000 cells/ml), the whole tissue cryopreservation group outperformed all the other methods.

A pot experiment was carried out to determine the effect of Serendipita indica on the salt response of Lolium multiflorum Lam. Although the salinity decreased the root colonisation of S. indica by 28.34%, successful colonisation of S. indica increased the seed germination rate, fresh weight, leaf relative water content and chlorophyll content by 28.09, 59.01, 38.78 and 28.80%, respectively, compared with uncolonised seedlings. Under salinity, leaf malondialdehyde content, leaf relative electrical conductivity, as well as Na+ content and Na⁺/K⁺ ratio in leaves and roots of S. indica-colonised seedlings were decreased by 33.99, 33.31, 63.40% and 47.42, 85.66 and 55.88%, respectively, compared with uncolonised seedlings. Meanwhile, compared with uncolonised seedlings under salinity, the contents of proline in leaves, N, P and K⁺ in leaves and roots of the S. indica-colonised seedlings were increased by 47.47, 45.69 and 30.05%, and 41.77, 19.51, 19.18 and 155.00%, respectively. These results indicate that S. indica colonisation confers salt tolerance in L. multiflorum seedlings by enhancing osmotic adjustment via actively accumulating proline and K⁺, increasing the uptake of nutrients such as N and P, and improving Na⁺/K⁺ homoeostasis. The study would provide a new idea for the combined application of salt-tolerant plants and symbiotic microorganisms in the ecological restoration of saline-alkali lands.

Scattered Greek juniper (Juniperus excelsa M. Bieb.) trees have ecological values, such as soil protection and soil erosion reduction in the Irano-Turanian region; however, intensive exploitation puts their habitats at risk. Therefore, knowing the soil characteristics of these habitats plays an important role in their management. The aim of this study was to investigate the soil quality of semi-arid woodlands dominated by a pure Juniperus excelsa community. At three sites in the Irano-Turanian region, soil samples were randomly taken from a depth of 0–20 cm in the eastern direction under the crowns of Greek juniper trees and from bare soil. The evaluated physical properties included bulk density (Bd), moisture, sand percentage, silt percentage and clay. Chemical properties included pH, electrical conductivity (EC), organic carbon (SOC), total nitrogen (TN), and nutrient concentrations of bare soil compared to the below-crown soil. Additionally, we investigated basal respiration (BR) and microbial biomass carbon (MBC) to evaluate the influence of Greek juniper trees on soil microbial activity. The bulk density value in samples taken under crowns was significantly lower by 42% compared to bare soil; however, significantly higher soil moisture (+39.8%) was obtained under tree crowns. Although clay and sand content under tree crowns was significantly higher than that of bare soil, sand content under tree crowns was significantly lower than in bare soil. According to our findings, soil pH under tree crowns and in bare soil was 7.13, which increased to 7.67 in bare soil. Soil EC (+15%), SOC (+76%), and TN (+29%) were significantly higher under tree crowns. The concentrations of magnesium and sodium were similar between bare and below-crown soils, but significantly higher phosphorus (+46%), potassium (+41%), calcium (+31.1%), iron (43.3), and zinc (+56.6%) were observed under Greek juniper crowns. Soil microbial activity was higher under tree crowns compared to bare soil, as evidenced by significantly increased BR (+49.1%) and MBC (+43.5%). Our findings indicate that scattered Greek juniper trees can improve soil properties, and their destruction leads to a significant decrease in soil quality. Generally, planting Greek juniper seedlings in degraded areas can help restore the soil quality.

The aim of this study was to evaluate the postoperative temporal changes in the tibial plateau angle (TPA) after a tibial plateau levelling osteotomy (TPLO) in dogs. The TPA of thirty-two dogs were measured before surgery (TPA₁), immediately after the TPLO (TPA₂), and later at the healing of the osteotomy site (TPA₃). Three observers carried out three TPA measurements for each patient at the time of the evaluation. TPA changes were observed over the bone healing (2.05 ± 3.05°); however, no statistical significance was noted when comparing TPA₃ and TPA₁. There were no statistical differences in the inter-observer measurements among TPA₁, TPA₂, and TPA₃ (P > 0.05). The comparison between the preoperative TPA₁ and the rock back showed a low positive correlation (S = 4 735.9, P = 0.471 5, r = 0.131 976 9). There was also a low positive correlation between the body weight (kg) and the change in the TPA during the bone healing (S = 4 581.8, P = 0.381, r = 0.160 234 7). Although changes in the TPA have been observed over the time of the bone healing, they were not influenced by the preoperative and postoperative TPA, or by the body weight. The magnitude of the variation in the TPA is not determined by the previous inclination or by the degree of rotation in the plateau.

The history of a site includes not only preserved historical buildings, but also areas with an assortment of plants, which have been preserved for many years. Rural settlements make up 80% of all the municipalities in the Czech Republic, with greenery covering 50-70% of their area. There are dominant trees and shrubs and an herb layer. The herb layer has high species diversity and species typical of the area, but at the same time, its long-term survival is in jeopardy. The continuity of rural settlements has been disturbed since the second half of the 20th century. Rural spaces often become uniform and impersonal, without links to the surrounding countryside, traditions, and history. This study included a total of 124 perennial families within three studied regions.

Aggregate Stability Index (ASI) and particulate organic matter (POM) are strongly influenced by land use and management. This work illustrates the effects of plantations on ASI and POM-C and POM-N in northern Iran. Three plantations of P. deltoides (PD), T. distichum (TD), A. subcordata (AS), and a fourth site ‒ adjacent abandoned lands (BL, as control) were selected. Soil samples were taken within 16 quadrats of each plantation and BL from the two depths of 0-15 cm and 15-30 cm during the summer. Soil C was significantly higher under TD (2.10%) than under BL (2.02%) > PD (1.61%) > AS (1.30%). Soil N was found in ranked order of AS (8.99%) > TD (7.82%) > PD (5.30%) > BL (3.68%) (P < 0.019). The significantly higher ASI was found under TD (57.49) in comparison with PD (53.10), BL (51.23), and AS (36.57). The POM-C was as follows: TD (0.209%) > PD (0.141%) > AS (0.139%) > BL (0.075%) (P = 0.020). The highest POM-N was found under TD (0.035), followed by AS (0.0284%), PD (0.0288%), and BL (0.007%). The results indicate the positive effect of afforestation on soil ASI and POM-C and POM-N, especially in the surface layers of soil.

In the current studies, heavy metal tolerance level, accumulation efficiency and sexual reproduction were determined in Viola × wittrockiana, a non-metallophytic ornamental cultivar in comparison to V. tricolor, a metallophyte, after zinc (Zn) or lead (Pb) treatment (0, 10, 100 and 1 000 ppm) in pot experiments. The seed germination frequency that was not reduced in comparison to the control, the effective Zn absorption from the soil and exclusion strategy for Pb, as well as the regular sexual reproduction of V. × wittrockiana treated with heavy metals all indicate the tolerance of this plant to heavy metals. The lack of a seed set under experimental conditions of V. × wittrockiana was due to the absence of pollinators, rather than the negative impact of heavy metals, as pollen viability and ovule development were normal under the treatments. The results indicate that V. × wittrockiana represents similar tolerance to Viola metallophytes and could be considered as a good material for the reclamation of polluted areas. The exceptional tolerance to heavy metals, the ability to initiate new generations in heavy-metal-burdened soil, which are additionally coupled with the unique beauty, make the garden pansy a good candidate to be potentially used in the future for phytoremediation purposes.

Despite recent issues, Norway spruce remains the most important commercial tree species which might be demanded henceforth for its broadly utilizable wood. Even before foresters faced both the bark beetle outbreaks and spruce decline, spruce monospecific stands were known to be prone also to other damage due to snow and wind. On this basis, measures that help prevent such failures were looked for, which resulted in the establishment of international IUFRO experimental series focused on impacts of different thinning regimes on stability and production of spruce stands. The thinning treatments differed in numbers of trees removed and retained on the site when dominant height of crop trees was reached or allowable cut in non-crop trees was accumulated. Also effects of different width of skid trails were tested. The study summarizes the results from the two IUFRO experiments in the Czech Republic. Effects of thinning regimes on spruce were found positive though thinning reduced the total volume production of wood while improving crop-tree stability which enhanced production safety. Different widths of skid trails had no effect on wood increment. Early thinning of spruce can be used to prevent their damage. No such measure, however, can alleviate the spruce decline.

The impact of precipitations and air temperatures on winter wheat yields was evaluated in a 34-year long- term field trial with mineral and organic fertilization established at two experimental sites with different soil-climatic conditions: Ivanovice na Hané with well fertile soils (degraded Chernozem), higher average year temperatures and lower precipitations; Lukavec situated in Bohemian-Moravian highlands with less fertile soils (Cambisol), lower temperatures and higher precipitations. At both sites, a significant positive effect of used fertilizers was noted from the dose of 80 kg N/ha; the best yields were generally obtained at 120 kg N/ha and 160 kg N/ha. The wheat yields at the Ivanovice site were negatively affected by the decrease of precipitations, namely in more fertilized treatments, particularly farmyard manure + mineral nitrogen, from the dose of 80 kg N/ha. A different trend was obtained at the Lukavec site where better winter wheat yields were obtained under lower precipitations. The air temperatures played a positive role at the Lukavec site, but no significant effect of temperature was observed at the Ivanovice site. The less productive areas in highlands can become more interesting for agriculture production with changing climate. However, the soils generally having lower quality and nutrient content can be a limiting factor for obtaining high yields.

In Turkey, almost half of which has semi-arid conditions, steppe areas have continuously been increasing. These areas need to be afforested to prevent desertification. For this purpose, this research presents specifically the Anatolian black pine (Pinus nigra J.F. Arnold subsp. pallasiana /Lamb./ Holmboe) as a suitable forest tree species for afforestation activities, due to wide distribution both in Turkey and the broader region. After a thorough investigation of past and recent literature, and onsite activities, the review focuses on the production of seeds and seedlings of the species, land preparation and planting technique, post-planting site maintenance, success in the afforestation areas and considerations on Anatolian black pine seed transfer regioning. In conclusion, local ecological conditions regarding the species should always be taken into consideration. Another important issue for the success of afforestation in semi-arid lands is that the preparation activities of the land for afforestation are carried out with appropriate techniques timely and painstakingly. Moreover, after the 2000s, Turkish forestry focused on afforestation in semi-arid and anthropogenic steppe areas. Anatolian black pine is the most widely used tree species in semi-arid lands and anthropogenic areas and plays the major role in successful afforestation.

The paper deals with the determination of the amount of logging residues including merchantable timber volume (brush) left in the beech stands for further use. Logging residues were recorded in all types of intentional felling starting with intentional improvement felling up to 50 years and ending with intentional regeneration felling. The potential amount of biomass left was divided into the share of brush and the share of small wood. The parameter was monitored for 3 years and all measured values were then statistically evaluated and related to 1 m3 of intentional felling in beech stands. A partial goal of this publication is characterization of economic impacts of the proposal for processing the recorded volume of logging residues left in the beech stands on the forest branch in Smolenice. The economic impacts are characterized in two proposed and mutually compared scenarios. The first scenario considers framework contractors for logging works with their own machines, i.e. by assignments, as a mediation of service invoiced for the assumed amount (m3) forwarded to the roadside landing, with the ownership of the forwarded dendromass belonging to Lesy SR (Forests of the Slovak Republic). Then, the dendromass is sold from the roadside landing to customers who are going to transport and process it at their own expense. The second scenario considers sales of assumed amounts of left dendromass including brush to customers who will provide for its transport and processing on the identified roadside landing within a set deadline under obligation to return the site to its original condition if there is possible damage. In this case, Lesy SR would be only a control body supervising labour quality and set-up technological procedures.

The goal of the study was to compare the dynamic changes in individual biometric indices of Scots pine in different natural zones of Ukraine, Polissya and Steppe. Scots pine stands were mainly concentrated in the Polissya zone, and their area was 3.6 times larger than that of forests in the Steppe zone, and the total wood stock by 4.6 times. The regression equations for biometric indices of artificial modal stands were developed. A comparison of the average height, diameter, and wood stock for pine stands of site index І^а, I, and II growing in Polissya and Steppe was made. It was found that the average differences in the average height (from 1.9 to 2.6 m) are observed at the age of 15-20 years. For the average diameter, the difference increases with age and the growth of the site index. The difference in the average stock is constantly increasing with age. At the age of 20 years this difference is 20-30 m³, and at the age of 120 years from 100 to 120 m³ depending on the site index. The developed growth models can be used in planning and prioritizing appropriate forestry activities for pine stands growing in specific regional conditions.

PRDX6 is a bifunctional protein involved in antioxidant regulation and phospholipid metabolism. Previous studies have shown that PRDX6 is involved in some biological pathways and networks related to lactation. The aim of this study was to explore the characteristics, function, tissue expression and variation of buffalo PRDX6 gene. We cloned and characterized the complete coding sequence (CDS) of buffalo PRDX6. The CDS of PRDX6 for swamp and river buffalo is the same, which consists of 675 nucleotides and encodes a protein of 224 amino acids. Buffalo PRDX6 contains one PRX_1cys functional domain (AA 7-222), which is probably related to the regulation of oxidative stress. Multi-tissue differential expression analysis showed that buffalo PRDX6 was highly expressed in the muscle, brain, lung and small intestine during non-lactation and lactation, and there were significant differences in expression in all the tissues except the small intestine between the two periods. It is worth noting that the mRNA abundance of buffalo PRDX6 in non-lactating mammary gland is higher than that in lactating mammary gland. Among the two single nucleotide polymorphisms (SNPs) identified in the CDS in this study, c.261C>T is shared by the two types of buffalo with different allelic frequencies, and c.426T>G is found only in river buffalo. The c.426T>G is non-synonymous, resulting in the amino acid substitution p.Asn142Lys. Only one nucleotide differential site is identified in PRDX6 gene between buffalo and other species of Bovidae. Phylogenetic analysis indicated that buffalo PRDX6 has a closer genetic relationship with that of the species in Bovidae. These results indicate that PRDX6 probably plays a crucial role in the mammary gland of buffalo. This study provides the foundation for further functional studies of PRDX6 in buffalo.

The aim of this study is to solve the forest park site selection problem using a Fuzzy analytic hierarchy process (FAHP) framework in the Galegol Basin, Lorestan province, Iran. The Delphi screening method was used to select the most relevant criteria and sub-criteria to the forest park problem. Using the FAHP weighting approach, the weight of each criterion and sub-criterion was calculated. Then, the suitability map of forest park location was mapped by the weighted linear combination (WLC) method. The results revealed that 7 criteria (climate, water resources, physiography, landscape, vegetation cover, wildlife and economic criteria) and 16 sub-criteria received the required values and can be involved into the decision-making process of the forest park site selection problem. Using the derived weights of sub-criteria by FAHP and the WLC method, the final results showed that most of the study area is moderately suitable for the forest park location problem. The results of this study can be valuable in the planning of local forest park and future land use planning.

Site conditions (topography, aspect, moisture availability, humus thickness, light exposure, and grazing activities) play a vital role in the germination and regeneration process. The research was conducted in the Himalayan moist temperate forest. The research site was divided based on the silvicultural system (group selection system and single-tree selection system) into 148 plots and 150 plots, respectively. The group selection system was examined on the site of 2 ha which was clear-felled under a project in the 1980's. The present study examined the impact of silvicultural systems on regeneration. The frequency table was used, and relative frequency was calculated for the species and silvicultural system, density per m2 was also calculated. Diversity indices were calculated through taxa, dominance, Simpson's index, Shannon index, evenness, equitability, and fisher alpha. Ten taxa were found in both silvicultural systems, with individual repetition of 17 and 15 taxa, respectively. Group selection is more compact visibly as compared to the single-tree selection system. The single-tree selection system is more diversified in species composition, stand structure, moisture availability, and less humus availability. The study also highlights future predictions for the conservation of these forests, which are highly sensitive and a hotspot for wildlife and climate change phenomena. Silvicultural practices such as silvicultural system, cleaning, weeding, thinning operations are regularly practiced, which can reduce the negative impact on these productive forests.

Knowledge of the molecular mechanisms of response to environmental stress is fundamental for the development of genetically stress-tolerant crops. This study aims to find vegetable soybean accessions tolerant to cultivation in stressful tropical environments. Fourteen accessions of the vegetable soybean (Glycine max (L.) Merrill) were grown in mineral and beach ridges interspersed with swale (BRIS) soils. The genetic diversity, estimated using inter-simple sequence repeat (ISSR) markers, revealed 42.50% polymorphism and was regarded as moderate. The unweighted pair-group method arithmetic average (UPGMA) analysis allocated the tested accessions into five major clusters at a similarity coefficient level of 0.43. The lowest values of the genetic distance were between IIUMSOY11 and IIUMSOY13 & IIUMSOY13 and IIUMSOY14, indicating that these accessions were more genetically distant from the other accessions. Ten differentially expressed proteins were identified in the three selected accessions IIUMSOY1, IIUMSOY11 and IIUMSOY14 using mass spectrometry, revealing a unique expression of the proteins involved in the storage, flavonoid metabolism, protein modification, oxidative stress defence, carbohydrate metabolism and respiratory chain. The findings may be valuable for the selection of genetically diverse accessions, to enhance the breeding of vegetable soybean genotypes suitable for stressful tropical environments.

The tillage depth plays a critical role in solving soil compaction - a global problem of soil degradation. However, to date, there are few research reported about tillage depth, and the standard of optimum tillage depth is lacking. Therefore, we conducted a meta-analysis to quantify the effect of tillage depths on crop yield across a global scale, and then to analysis their influence factors such as local climate, soil properties, and managements. Moreover, a global distribution of the optimal tillage depths was estimated by using a random-forest model. Overall, our result demonstrated that crop yield first increased within tillage depths from 25 to 35 cm, and then reduced under higher depth of deep tillage compared to conventional tillage, according to 1109 wheat, maize and soybean (WMS) yield observations from 202 studies and 109 publications. Visibly, 35 cm hence became the optimum tillage depth of WMS across the world, while it varies with different regions. Furthermore, higher crop yields observed in areas with a humid climate, high clay contents, and large bulk density under the optimal depth 40, 35 and 45 cm, respectively. In contrast, a lower yield was observed in areas with arid climates, silty and sandy soils, and lower bulk density within optimal depth of 25 cm, 30 cm, and 25 to 35 cm. Human management efforts, including fertilizer addition, irrigation, straw returning, and changing of cropping system or crop species mostly increased the crop yield under deep tillage. Particularly, our meta-analysis indicated that straw returning needs a greater depth. Finally, we predicted the distributions of optimum depths, which showed that 30 and 35 cm were the optimum tillage depths in the temperate and tropical regions, and the total crop yields of global WMS increased by 2689 million tons per year under the optimal tillage depth, compared with the conventional tillage.