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The nutritional status of winter oilseed rape (WOSR) during its vegetative period is crucial for plant growth and can be used for the seed yield prediction. This hypothesis was verified based on the data from long-term field experiments. The experiment consisted of four potassium (K) treatments based on the progressive K supply potential to plants from soil and fertilizer and two magnesium treatments (-Mg, +Mg) conducted in 2013-2015. The content of nutrients (N_tot, P, K, Mg, Ca, Fe, Mn, Zn, Cu) was determined at the rosette stage (BBCH 30) for leaves and separately for leaves and stems in the late stage of inflorescence growth (BBCH 57-59). The low K content appeared as the key limiting nutrient in WOSR plants in the rosette stage due to the insufficient soil fertility level, depended even more on weather conditions. This negative K nutritional trait persisted through the whole vegetative WOSR growth. Its detection was possible, because stems were included in the diagnostic procedure. The most reliable prognosis of WOSR yield was conducted based on the nutritional status of stems in the late stage of the inflorescence development.

The aim of this study was to evaluate the applicability of B-mode, Doppler, acoustic radiation force impulse elastography and contrast-enhanced ultrasound exams in the differentiation of neoplastic and non-neoplastic benign mammary lesions in bitches. This research was conducted as a prospective secondary observational cohort (2014-2016) study, which included 36 mammary lesions, evaluated physically and by ultrasound (B-mode, Doppler, contrast-enhanced ultrasound and acoustic radiation force impulse) exams prior to mastectomy and histopathological classification as neoplastic or non-neoplastic lesions. All ultrasonographic parameters studied were compared between histopathological classifications using Fisher's or Student's tests and differences were considered significant when P < 0.05. Out of 36 benign mammary lesions evaluated, 25 were classified as neoplastic and 11 as non-neoplastic. The qualitative and quantitative parameters evaluated using the different ultrasound methods were not effective (P > 0.05) in differentiating between neoplastic and non-neoplastic mammary masses in bitches. Nevertheless, some B-mode variables, such as longitudinal length (P = 0.0292), width/length ratio (P = 0.0001) and width/height ratio (P = 0.0001) showed limited efficacy in the differentiation of mammary lesions types. In conclusion, ultrasonographic evaluation of benign canine mammary lesions did not allow differentiation between neoplastic and non-neoplastic tissues and only a few B-mode variables may aid in the prediction of specific tumour types.

The aim of this study was to evaluate the effectiveness of deslorelin acetate in the regulation of reproductive activity in captive leopard geckos (Eublepharis macularius). Fourteen healthy adult females were separated into two groups. Under general anaesthesia, deslorelin acetate implants (4.7 mg) or placebo implants were administered into the coelom of ten female geckos and four female geckos, respectively. One healthy adult male Leopard gecko was added to each group of females (five females with GnRH implants and two females with placebo implants). The geckos were regularly monitored over two breeding seasons (visual examination, weight control). Nesting sites were checked daily. There were no postoperative complications or any other health problems during the study. Implant administration did not result in long-term suppression of reproductive function. No significant differences were found in the number of clutches between the female groups (deslorelin implants versus placebo implants) or in the number of clutches between the two breeding seasons. Deslorelin acetate implants did not interfere with ovarian activity in captive female leopard geckos. The use of GnRH agonist implants is not an appropriate method for control of reproductive function in female leopard geckos.

European fruit lecanium, Parthenolecanium corni (Bouché, 1844) is among the leading worldwide pests of fruits such as plums, currants, jostaberry, grapevine and many others. This study is focused on testing the effectiveness of natural insecticide treatments compared with conventional insecticides on overwintering nymphs of the European fruit lecanium. In February of 2017, two experiments were conducted under laboratory conditions. The tested products were Boundary SW^® (plant extract), Konflic^® (plant extract), Ekol^® (canola oil) and a tank-mix of Ekol^® + Reldan 22^® (canola oil and chlorpyrifos-methyl) as a chemical standard. The most effective product tested was the chemical standard tank-mix of Ekol^® and Reldan 22^®, which was 100% effective in all experiments. High efficiency was also achieved with Ekol^® (canola oil) in high concentrations. We can summarize, that using canola oil in high concentration has almost the same effect as the chemical standard. Using canola oil against European fruit lecanium meets the principles of Integrated Pest Management (IPM) and also the need to design cropping systems that are less dependent on synthetic pesticides.

Milk production is one of the most important areas of the Czech agrarian sector, as evidenced by its 50% share (at 2017 prices) in revenues from livestock production. As for any business, a certain level of profitability is a prerequisite for long-term and sustainable development of dairy farms. This study's aim was to evaluate the economic efficiency of milk production from both Czech Fleckvieh (C) and Holstein (H) cows based on data collected each year from 48 to 70 Czech dairy farms in the period from 2012 to 2017. Total costs per feeding day and litre of milk, level of profitability, and income over feed costs were calculated. The influences of herd size and milk yield on profitability and break-even points were examined while sensitivity analysis and model calculations were utilised to predict profitability. The farms with higher average milk yields (>7 500 and >9 500 L per lactation for C and H, respectively) had higher costs per feeding day, lower costs per litre of milk, and improved profitability (p < 0.05). Average break-even points were estimated for milk price (0.31 and 0.32 EUR) and milk yield (7 257 and 9 209 L) in C and H herds, respectively.

In June 2018, the presence of one adult specimen and one egg cluster of the North-American oak lace bug Corythucha arcuata were recorded near the village of Mužla, close to the Danube River, in southern Slovakia. They were found on leaves of Quercus cerris growing in a mixed stand. In August 2018, other specimens (nymphs and adults) were observed on Quercus robur leaves, near Čičarovce, in eastern Slovakia. This is the first record of C. arcuata in our country. The oak lace bug probably reached us while invading from Hungary, where heavy infestation is reported from several parts of the country.

The antioxidant properties of cool-climate Regent wines from Podkarpacie region, Poland were investigated. Total polyphenols, total flavonoids, total monomeric anthocyanins, trans-resveratrol concentration, radical scavenging ability using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and total antioxidant capacity with TEAC and FRAP methods were measured. The average contents of the compounds determined for Regent wines were comparable to the respective data available in literature found for warm-climate wines. Correspondence Analysis of the data revealed the relation of total polyphenols content and antioxidant capacity and radical scavenging ability of studied wines, and in lesser extent correlation with total flavonoids content. The total monomeric anthocyanins content and resveratrol concentration were poorly related to the total polyphenols content and antioxidant capacity.

To determine the best phosphorus (P) fertilizer management strategy in chernozem soil in the semi-arid region of Northeast China, a field experiment under film mulched drip irrigation was conducted for two consecutive years. Five P application methods were tested, including no P fertilizer applied (P0); P fertilizer one-time basal application (P1); one fertigation one time (P2); fertigation twice (P3) and fertigation four times (P4). The shoot dry matter weight, phosphorus accumulation, yield and POlsen in soil were measured regularly during the maize growing season. The results demonstrated that P fertilizer application methods imposed significant effects on dry shoot matter, the apparent P loss, P fertilizer use efficiency and yield of maize (P < 0.05). The yield, P agrinomic efficiency and P recovery efficiency of P4 treatment were significantly higher than P1 treatment by 4.2, 39.7, and 66.4% across two year. In addition, P4 treatment significantly enhanced the shoot dry matter weight after V12 stage, post-silking P uptake and reduced the apparent P loss. In conclusion, P fertigation and a rational frequency (e.g., fertigation four times) enable to keep the maximum grain yield, the shoot dry matter, and recovery efficiency of P fertilizer (66.4%) though changing agronomic methods for efficient acquisition of P toward a sustainable and productive agricultural fertigation system.

Autologous lamellar corneal grafts were performed on four dogs with two deep corneal ulcers and two corneal perforations to achieve better visual and aesthetic outcomes. The donor corneal graft was harvested from the relatively normal corneal region of the affected eye and used to cover the corneal defect. At the two-year follow-up examination, ultrasound biomicroscopy was performed to confirm the thickness and endothelial continuity of the transplanted grafts and donor site of the cornea. The evaluations revealed that the dogs had healed without incident and that their vision had been maintained. Furthermore, the owners were satisfied with the aesthetic outcomes in all cases. Our findings show that autologous lamellar corneal grafts are useful and effective in treating canine corneal ulcers and perforations.

Techniques of drip irrigation are broadly applied for the reclamation of saline-alkali lands, during which effective management of water use to accelerate salt leaching is essential for crop production. In 2017, a field experiment with five treatments of soil matric potential (SMP) levels of -5, -10, -15, -20, and -25 kPa was conducted in heavy saline silty soil land in Bohai Bay, China to study the effects of drip irrigation on salt leaching. The results showed that salt leaching was enhanced with increasing SMP, particularly under an SMP of -5 kPa within a 30 cm soil profile depth and 15 cm distance from the dripper, and the average electrical conductivity of saturated paste extracts (ECe) decreased from 13.8 to 1.52 dS/m. Water consumption increased with increasing SMP, but the yield of oil sunflower did not differ significantly between SMPs of -5 and -10 kPa. These findings indicated that a relatively high crop yield of oil sunflower and effective salt leaching can be achieved if the SMP can be controlled at -10 kPa in heavy saline silty soil.

The aim of the research was the determination of the tolerance of different pear grafting combinations to various levels o lime concentration in calcareous soils. The experiment was carried out under pot conditions for a two-year period (2015-2016). Two factors including combination of rootstocks/scions (3 × 3) and levels of lime concentration were employed to evaluate leaf responses including total iron (Fe), available Fe, chlorophyll (Chl) (a, b, total), and chlorophyll fluorescence attributes (F0, Fm, Fv/Fm) as well as annual growth and internode length of current shoots. Results showed that soil lime significantly reduced tree growth but conversely intensified iron chlorosis. Also, contrary to our expectations, the Pyrodwarf/Pyrus communis L. (Dargazi) combination displayed more tolerance to high lime concentrations, whereas the OH × F/Williams Duchesse combination did not exhibit suitable tolerance. As a pear rootstock native to Iran, Dargazi seedling rootstock in combination with different scions was found to have relatively better growth under low lime concentrations, but its response under high lime was not as favourable as expected. Cultivar Dargazi combined with different rootstocks showed a better response to high lime stress compared to other scions, while cultivar Williams Duchesse exhibited lower tolerance to high lime concentration when combined with different pear rootstocks.

Common wheat, a fundamental commodity on international markets, is increasingly differentiated into commercial types on domestic markets to meet the demand of processing companies. Improver wheat, biscuit wheat, ordinary and superior bread-making wheat are commercial varieties with specific technological characteristics. Wheat farming systems are constantly evolving, and as a result, related environmental issues emerge. We applied an LCA (Life Cycle Assessment) analysis, where the functional unit was 1 tonne of grain for each typology and system boundaries were from cradle to farm gate. Primary data were used in the study, and special attention was paid to fertiliser use. From an LCA perspective, our findings show that nitrogen (N) plays an essential role in plant production although producing different waterborne and airborne emissions and nitrate leaching, for the 4 commercial typologies studied. Furthermore, the impact can be differentiated based on the technological features of the commercial types. Our results led us to observe that the four wheat types show contrasting economic and environmental performances.

The objective of this study was to evaluate potential changes in intraocular pressure in spontaneously breathing dogs undergoing laparoscopic ovariectomy with capnoperitoneum. Twenty bitches undergoing laparoscopic ovariectomy were enrolled in a prospective clinical trial. The dogs were under general anaesthesia and were breathing spontaneously throughout the entire period of the procedure. Capnoperitoneum was then established with an intra-abdominal pressure setting of 10 mmHg. Collected data included intraocular pressure, pupil size, heart rate, respiratory frequency, systolic and diastolic arterial pressure, end-tidal CO₂, oxygen saturation of haemoglobin and tidal volume. Data were recorded 5 min before starting CO₂-insufflation (baseline), after capnoperitoneum establishment (T0), and at further 5-min intervals (T5, T10, T15, T20, T25, T30). Compared to baseline, pupil size was increased at T30 (P = 0.03) and respiratory frequency at T25 (P < 0.01) and T30 (P = 0.02). No other significant changes were found regarding intraocular pressure. Our data show that the induction of an intra-abdominal pressure of 10 mmHg for a 30-min laparoscopy with the use of medetomidine-butorfanol-propofol-isoflurane in spontaneously breathing dogs in the horizontal position does not result in any important changes in intraocular pressure or end-tidal CO₂.

Shoulder joint disorders are a major cause of forelimb lameness in dogs, and osteochondrosis, degenerative joint disease, and bicipital tenosynovitis are common joint disorders that have been reported in dogs. Many studies have investigated pain management after arthroscopy in human medicine, but reports from veterinary medicine are rare. Ropivacaine is a new amide local anaesthetic drug and a single isomer drug that is used more widely than bupivacaine in human medicine because it has fewer side effects. The present study was conducted to evaluate the analgesic effect of intra-articular injection of ropivacaine after arthroscopic surgery in dog shoulder joints. To accomplish this, ten dogs were randomly divided into two groups of five who underwent the same anaesthesia protocol and shoulder arthroscopic examination. After shoulder arthroscopy, ropivacaine or 0.9% NaCl was injected into the shoulder joint cavity and the dogs were evaluated at one hour, two hours, four hours, six hours, 12 hours and 24 hours after surgery. The evaluated parameters were heart rate, respiratory rate, lameness score, visual analogue scale and the short form of the Glasgow composite measure pain scale. Ropivacaine showed a higher analgesic effect than 0.9% NaCl, indicating that it may be useful for pain management following arthroscopic surgery in dogs.

The present study aimed to evaluate the effect of two dietary Ca levels (3.00% vs 3.50%) in three hen genotypes (ISA Brown, Bovans Brown and Moravia BSL) on their laying performance and quality of their eggs. In order to evaluate the response to dietary Ca, an experiment was conducted with 300 laying hens. One hundred birds from each genotype were divided into 10 replicates (10 birds/cage/Ca level). To reveal the differences, the following characteristics were measured: hen-day egg production percentage, daily feed intake, egg weight, albumen and yolk weights, Haugh unit, eggshell colour, eggshell weight, eggshell thickness, eggshell strength, egg shape index and eggshell index. A higher Ca level significantly increased the hen-day egg production percentage of Bovans Brown and Moravia BSL, while ISA Brown laying hens had the higher egg production percentage at a lower dietary Ca level (85.97% vs 82.41%). No significant effect of dietary Ca level was observed on any internal egg quality measurements except the Haugh unit. However, the dietary Ca level significantly affected some of external egg quality measurements, namely eggshell colour (P < 0.001), eggshell weight (P = 0.003) and eggshell strength (P = 0.012). The results of the present study show that laying performance and egg quality parameters responded differently according to hen genotype and dietary Ca level. Bovans Brown and Moravia BSL laying hens required higher levels of Ca to keep egg production high, while the eggshell quality measurements were better at the lower dietary Ca level. Thus, it is recommended to adjust the Ca levels in feed to the nutritional requirements of laying hens.

In raspberry, the expression of the primocane fruiting trait is influenced by the environment. Although there are several factors that influence the expression of this character, it is well known that low temperatures that occur during the growth season of the primocane, are important and affect the flowering. In this study, plants in their early stages of development were exposed to low temperatures (2°C) for one month, in a dark cold chamber. The following genotypes primocane and floricane were used: 'UC103', 'Autumn Bliss', 'Heritage', 'Meeker' and 'Tulameen'. Flowering and growth were recorded until the end of the season and the morphology of the meristem was characterized in this moment. Interaction between cold and genotype was detected in all parameters studied. In 'Heritage', a slight primocane, growth and flowering were favoured by exposure to cold. Thereby, low temperature affects flowering, but this effect depends on primocane fruiting degree of each genotype, slight primocane the cold favored flowering and growth. However, strongly primocane the cold had no effect on flowering and growth.

The paper is devoted to an analysis of the development of agrarian farms in Visegrad Group (V4) countries, primarily in terms of results and the most important production inputs of production factors and their efficiency in the period from 2004 to 2013 based on the EU Farm Accountancy Data Network (FADN). The results of the analysis show that if farms in the V4 countries want to achieve the same performance as developed countries, they must invest more in purchasing intensification factors and adjust the structure of assets production specification. It will be necessary to stop the reduction in the numbers of livestock and to strive for growth in gross farm income, mainly through the processing of agricultural raw materials.

Soil erosion is one of the major environmental problems in open-cut mines in tropical regions. It causes negative impacts including the removal of nutrient-rich topsoil, destroys aquatic habitat, dam and pond siltation, clogs river by deposition of sediment, and causes water pollution in the rehabilitation process. Soil texture is an important factor to affect soil erosion. In this study, artificial rainfall experiment in the laboratory scale was conducted to clarify the mechanism of soil erosion under the different soil composition and to discuss the methods for minimizing soil erosion. The obtained results showed that the soil seal generated due to the presence of fine particle under high rainfall intensity is the main contributor to accelerate the soil erosion. Additionally, the surface coverage by the cover crops is the most effective measure to reduce soil erosion because both the coarse and fine contents runoff can be minimized while arranging of the slope angle is effective for reducing the runoff of coarse contents and the soil compaction is effective to reduce that of fine contents. Soil erosion can be minimized by selecting prevention method considering the type of soil because the prevention effect on soil erosion is different depending on the type of soil.

The purpose of this research was to find a suitable protocol to enhance frozen rabbit sperm preservation analysing the role that seminal plasma (SP) plays and the effect of different cryoprotectant agents on sperm quality 0 and 2 h after thawing. Sperm samples were pooled and divided in eight fractions. Four of them were diluted with BotuCrio^® (extender A), INRA 96^® plus 6% glycerol (extender B), 6% N, N-dimethylformamide (extender C) and 6% N-methyl-2-pyrrolidone (extender D), respectively. The other four fractions were centrifuged and the supernatant was discarded in order to eliminate SP. Each sample was then resuspended with extender A, B, C and D. Samples were cooled progressively, loaded into 0.5 ml freezing straws and frozen with liquid nitrogen vapour. Thawing was performed by placing the straws into a bain-marie at 37°C for 21 s. Straws were dried and sperm samples placed into Eppendorf tubes to be analyzed by ISAS software, vitality test, HOS test and acrosome integrity test. The best motility and velocity parameters were obtained by extender A (P < 0.050) even when the motility parameter was compared with previous studies using other diluents. Additionally, sperm quality decreased over incubation time (P < 0.050) and no differences were found in samples processed with or without SP. This research revealed that BotuCrio^® could be used for rabbit sperm cryopreservation and moreover the improvement of the cryopreservation process of rabbit sperm due to the demonstration that SP removing is not required.

One of the important issues both in the political discussion about climate change and in forest ecosystem research is carbon sequestration. In this paper, we estimated soil carbon sequestration (SCS) in two Persian oak forest stands of different origin (seed and coppice). Soil samples were taken at two soil depths (0-15 and 15-30 cm) and locations (under the tree crown and open area) in each oak stand. Results showed that surface layers (0-15 cm) had the highest soil carbon sequestration ranging from 41.2 t.ha-1 to 47.9 t.ha-1 for both oak forests. The total SCS was higher (between 79.5 and 89.07 t.ha-1) in open areas of the two forest stands than under the crowns of oak trees. Finally, the amount of total SCS in seed originated forest (SOF) (86.52 t.ha-1) was significantly greater (P < 0.05) than in coppice forest (CF) (77.70 t.ha-1). The results indicate that a relatively large proportion of C loss in CF is due to overgrazing, forest degradation and conversion to coppice forests in the study area.

Onion skin is a waste produced in thousands of tons annually in the European Union. It is a rich natural source of flavonoids and its water extracts (as an environmentally friendly solvent) could be used as an antioxidant material for meat products. Therefore, antioxidant properties of onion skin water extracts (OSWEs) were tested on cooked pork patties. Pork patties were divided into five treatments: control (no antioxidant), 10 and 20% (w/w) of yellow OSWE, and 10 and 20% (w/w) of red OSWE. Antioxidant activity, total polyphenols, thiobarbituric acid reactive substances (TBARS), and sensory analysis were assessed. Patties with added antioxidants showed significantly (P < 0.05) higher antioxidant activity and total polyphenol content. Samples with OSWEs, after 5-day storage (5°C), had significantly (P < 0.05) lower TBARS values compared to control. Two main phenolic compounds were identified in OSWEs by liquid chromatography - mass spectrometry using electrospray ionisation in negative mode: quercetin (m/z 301) and quercetin monoglucoside (m/z 463). OSWEs demonstrated the potential to be used as a source of natural antioxidants with strong antioxidant activity in meat products.

An intracardiac communication between the left ventricle and the right atrium (Geborde defect) was diagnosed in a 9-year Yorkshire Terrier with a history of chronic exercise intolerance. The history, clinical examination, and diagnostic imaging confirmed the diagnosis and did not reveal evidence of trauma or endocarditis which could lead to this special type of left-to-right shunting. A Gerbode defect is a very rare finding in human beings and animals. In the veterinary literature all reports about this condition were related to thoracic trauma or valvular infection. According to the authors, this would be the first clinical case of congenital Geborde defect in a dog.

Mechanical transplanted seedling must meet the requirements of standard seedling block with uniform distribution of seedlings and inter-twisting roots for rolling. This study was conducted to identify the effect of growing media on mat type seedling raised for mechanical transplanting at Bangladesh Rice Research Institute (BRRI), Gazipur during the period of 2012-2014 covering two dry and cold seasons (Boro) and one wet season (Aman). Seedling were raised on plastic tray using sandy loam and clay loam soil mixed with decomposed cow-dung, mustard cake, rice straw organic fertilizer, rice bran, poultry litter and vermicompost at the rate of 0.0, 10, 20, 30 and 40%. Rolling quality of the seedling mat decreased and seedling height increased with the increased of mixing rate of organic fertilizer except rice bran and mustard cake. Averaged across three seasons, 10 to 30% cow-dung, rice straw organic fertilizer, vermicompost, 10% poultry litter and 20 to 30% rice bran with both types of soil was found suitable for seedling mat and seedling height. However, seedling varied among the organic fertilizers with both types of soil in the order of cow-dung > rice bran > vermicompost > poultry liter > rice straw organic fertilizer > mustard cake. Clay loam soil showed better performance on rolling quality over sandy loam soil.

Research in southern Italy assessed the effects of biodegradable mulch on fruit yield and quality of two greenhouse tomato cultivars, 'Coronel F₁' and 'Kero F₁'. Three mulching types (two MaterBi biodegradable black films, MB N2/12 amnd MB N8; black polyethylene film, low-density polyethylene (LDPE)) and not mulched control were compared. 'Coronel F₁' showed higher values of fruit yield, total crop biomass and leaf area index (LAI). MB N8 and LDPE films led to the highest fruit yield and growth indexes, whereas not mulched control to the lowest. Fruit dry residue and soluble solids were highest under MB N2/12 and MB N8, titratable acidity was highest under MB N8. Fruits grown under MB N8 and LDPE mulches attained the highest levels of colour components "L" and "b" respectively, and MB N8 the highest fruit firmness. MB N2/12 and MB N8 showed the highest levels of antioxidants and antioxidant activity. Biodegradable polymers improved root growth conditions and fruit quality, showing suitable features for sustainable vegetable production.

A public private partnership can be an effective approach to deal the projects with modern agricultural development in Sub Saharan Africa. A former financial analysis of a development project, carried out by the authors, showed that public and private partners can effectively join in a mutually satisfactory venture capital. The same project is now complemented with a bankability study, considering lenders options, equity allocation, collaterals and likely applicable interest rates, available cash flow and sustainable debt service repayment to provide a through financing scenario for each partner's perspective assessing the relevant Debt Service and Loan Life Cover Ratios. Cash flow and interest rates fluctuation impacts are eventually investigated with a sensitivity analysis to prove the robustness of the proposed scenario.

Pot experiments were carried out to study the effects of a novel stabilizer of 2,4,6-trimercaptotriazine, trisodium salt, nonahydrate (TMT) on the bioavailability of heavy metals (Cu, Zn, As, Cd and Pb) in soils and heavy metals accumulation in tobacco. The results showed the optimal TMT dosage 200 mL/kg could effectively reduce the bioavailability of heavy metals in soils, and the bioavailability of Cu, Zn, As, Cd and Pb in soils was reduced simultaneously by up to 73.1, 63.2, 48.0, 68.9 and 57.2%, respectively. Application of TMT could significantly decrease Cu, Zn, As, Cd and Pb contents of all parts of tobacco. Furthermore, the contents of available Cu, Zn, As, Cd and Pb in soils were significantly positively correlated with Cu, Zn, As, Cd and Pb contents in upper, middle and lower leaves and stems of tobacco. There were no significant differences among plant height, number of leaves, stem girth, length and width of maximum leaves of tobacco under TMT treatments and that under control (P < 0.05), which showed that the TMT did not promote and inhibit the growth of tobacco.

Based on a long-term fertilization experiment on sandy soil, research concerning the effect of variable fertilization on the soil content of silicon extracted by CaCl₂ solution was performed. The content of plant available silicon was evidenced to depend on the applied fertilization and soil properties. Plant silicon supply coefficient varied depending on the fertiliser combination. In the case of potatoes this coefficient was smaller then 1 but for oat was usually higher then one. It suggesting that the amount of available silicon in soil is sufficient to cover the nutritional needs of potatoes but not sufficient for oat.

Athletes, including equine athletes need high intensity training in order to achieve high performance. However, continuous high intensity training often results in injuries to the locomotive system. The buoyancy of water reduces the force born on joints; therefore, training in water has been used for rehabilitation purposes both in humans and horses. The few studies dealing with water treadmill training of horses suggest that the fitness of horses can be improved using this training method, but none tested the subsequent performance of horses after water training. Therefore, the aim of this study was to test the effect of water training of varying intensity on the fitness-related parameters of show jumpers during training and after competition. Four similarly trained show jumper sport horse (aged between 7-11 years) competing at the same level (110 cm) were selected. Horses were subjected to 44-min deep water treadmill training with three intensities (9, 11, 13 km/h maximum speed) three times a week in addition to their normal training. At the conclusion of the week, horses participated in a two-day indoor show jumping event. Blood samples (4 ml) were taken from the jugular vein during the third water training and before and after the completion of the show jumping course on each day. From the blood plasma, lactate dehydrogenase (LDH), creatine kinase (CK) and aspartate aminotransferase (AST) activities, as well as lactate, glucose and triglyceride levels were determined. Data analysis was carried out with SAS (SAS Inst. Inc., Cary, USA) using the GLM procedure and Duncan's new multiple range test. Pearson correlation coefficients were calculated between the same blood parameters from different sampling times. No interactions were detected between training intensity and sampling time during water training. Plasma lactate and glucose levels were decreased during the water training, while values increased afterwards. In contrast, heart rate, triglyceride and cortisol levels were elevated as a result of water training. Increasing the maximum speed of the water treadmill had no influence on the average heart rate of horses subjected to the training. Plasma lactate levels decreased with the increased maximum speed of the water trainer. Activities of AST, CK, LDH, and levels of cholesterol, cortisol and bilirubin decreased when the maximum speed of the treadmill was set to 11 km/h compared to the 9 km/h training. Water training resulted in lower heart rate measured right after completing the show jumping course when horses were subjected to medium intensity water training. The AST, CK and LDH activities measured before and after water training had only weak to moderate positive correlations with values measured after competition. In conclusion, our results indicate that deep-water training alters the biochemical processes and can improve the aerobic energy supply of show jumpers. Water training is a strenuous exercise, which initially leads to increased muscle damage. However, this initial phase is followed by subsequent adaptation.

The aim of this study was to evaluate the response pattern of diazotrophic microbes, denitrifiers and nitrifiers to different types of land use management, such as soybean monoculture (M) during 5 and 24 years (M5 and M24) and soybean-maize rotation (R) during 4 and 15 years (R4 and R15) in two subsequent years at the time point of flowering. Soil samples from a site recently introduced into agriculture (RUA) and a pristine soil under native vegetation (NV) were used as controls. Abundances of different functional groups of microbes were assessed using the direct quantification of marker genes by quantitative real-time PCR using extracted DNA from rhizosphere samples. In addition, soil chemical and physical properties were analysed and correlated with the abundance data from the functional microbial groups under investigation. Overall, the results indicate that the abundance of nifH genes was higher under R treatments compared to M treatments. The abundance of ammonium monooxygenase genes amoA (AOA) was generally higher under rotation systems and decreased under M24. RUA evidenced a negative effect on the establishment and development of AOA communities. The influence of land use on nirS abundance was inconsistent. However, R treatments showed a high abundance of nirK genes compared to M treatments. In both growing seasons, the abundance of nosZ genes was higher under NV compared with the other treatments. Furthermore, M24 treatment was related to strongly changed chemical and physical soil properties compared with the other sites. As expected, soil samples from RUA showed the strong dynamics of measured parameters indicating the high sensitivity of soils under transition to environmental parameters. Our results also indicated that the long-term crop rotation modified the abundance of the investigated microbial groups compared to the monoculture and increased soil chemical and physical quality. Therefore, our results provide evidence for a stimulatory effect of the long-term crop rotation on the abundance of microbes involved in N transformation.

In a two-year pot experiment, the effect of five growing media on the growth, flowering, decorative value of Pelargonium peltatum cv. Maxime as well as on their uptake of the nutrients and heavy metals were studied. The media were prepared from four composts (made from: sewage sludge 70% or 35%, potato pulp 35%, straw 30% or sawdust 30%) and peat in 1:1, V:V ratio. In the 1^st year of research 7-month-old composts and in the 2^nd year 18-month-old composts were used. Plants cultivated in 7-month-old composts showed better growth-related parameters, created more inflorescences and were more decorative than those cultivated in 18-month-old ones. The medium with compost consisting of 70% sewage sludge and 30% straw gave the best results. Composts application increased nutrients and heavy metals content in pelargonium leaves. Heavy metals content was definitely lower than the value considered toxic to plants.