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A field trial with four potato cultivars (Faluka, Manitou, Madeleine and Stirling) was conducted in two consecutive years (2012 and 2013) at a laboratory field of Biotechnical Faculty in Ljubljana. The aim of the study was to evaluate different requirements of the selected cultivars in regard to the ridge and tuber cluster. The following parameters were defined and monitored: area of the form surrounding the tubers (ellipse), cross-sectional area of the ridge, vertical and horizontal tuber span in the ridge, the length of semi-axes (a) and (b) of the ellipse, minimum distance of tubers and ellipse from the outer ridge side. The results of 2012 trial indicated that the minimal ellipse method defining the ellipse was not satisfactory as statistical significance was limited due to a large volume of vacant space in the ridge not occupied by potato tubers. Therefore, the mathematical model was upgraded in 2013 and a physical parameter (tuber mass) was incorporated in the equation to better depict the tuber cluster. The trials were designed as a randomized block with five repetitions. The largest ellipse, horizontal tuber span in the ridge and the longest semi-axis (a) were recorded for the cv. Manitou resulting in the highest yield. However, no statistical differences were recorded in the horizontal tuber span, the length of the semi-axis (a) or cross-sectional area of the ridge among cultivars analysed. It was determined that different cultivars require specific growth space in the potato ridge. Moreover, the newly developed method proved efficient for determining growth requirements of potato tubers in the ridge.

A rare case of a liver biliary duct cystoadenoma in a dog is reported. Tissue changes are described with an emphasis on immunohistochemical patterns that aid in the identification of the tumour and differentiation from similar macroscopical features that arise due to congenital abnormalities. Finally, we provide hints on comparative pathology.

A total of 120 weanling pigs [(Landrace × Yorkshire) × Duroc], 21 days of age with an average initial body weight (BW) of 6.52 ± 0.22 (SE) kg were selected to investigate the effects of extruded rice product as a replacement for dried whey on growth performance, coefficient of total tract apparent digestibility (CATTD), blood profiles, faecal shedding of Lactobacillus and Escherichia coli (the microbial counts of digesta were expressed as log₁₀ colony forming units per gram), and faecal scores of weanling pigs. Pigs were randomly allocated to one of four dietary treatments, with six replicates per treatment and five pigs per pen. Pigs in the control group were fed a diet based on corn, soybean, and 20% dried whey. Experimental groups received the same diet as the control group, but dried whey was replaced by 3%, 6%, and 9% extruded rice. Throughout the experimental period (six weeks), no differences were observed in the average daily gain, average daily feed intake, and gain/feed ratio. Pigs fed 6% and 9% extruded rice had a lower (P < 0.05) CATTD than pigs fed the control diet on Day 14. The blood creatinine concentration of pigs fed 9% extruded rice was higher (P < 0.05) than that of pigs in the control group on Day 14. Pigs fed the diets containing 6% and 9% extruded rice had decreased (P < 0.05) faecal E. coli counts on Day 14 compared with pigs fed the control diet. The current results indicate that feeding extruded rice can decrease faecal E. coli counts without negative effects on growth performance in weanling pigs.

Compost leachate is produced by conversion of municipal waste to compost. It contains significant amounts of organic materials, nutrients, soluble salts and small amounts of some heavy metals. Adding compost leachate to the soil can pollute it, and also improve soil fertility. In this study, we investigated absorption and growth performance of Populus deltoides under irrigation with leachate of the Compost Plant of Rasht. At the beginning of the growing season, in mid-March 2013, cuttings were processed from young, one-year-old seedlings of Populus trees in the greenhouse of the Safrabaste Poplar Research Station. Cuttings were planted in pots filled with loamy-sandy soil in the vicinity of the area with 40cm depth. Compost leachate was taken from a collection reservoir where leachate from the open composting of organic municipal wastes and various gardening and plant wastes had been collected. Five treatments were applied to plants: (C) tap water (control), (P) pure leachate, (3:1) three units (by volume) of leachate mixed with one unit of tap water, (1:1) and (1:3). The growth of tree diameter and height was measured bi-monthly. Aboveground (stem, branches and leaves) and root biomass was inventoried at the end of the growing season. Concentration of elements was determined every three months. Overall, growth parameters in 1:1 and 1:3 treatments were similar together and there were no significant differences between them (P < 0.05). Pure treatment had minimum growth because of a high concentration of solvable salts in leachate such as sulphur that causes salinity and high EC in soil. Treatment1:3 exhibited the greatest overall aboveground and root dry mass. Total aboveground dry mass of leachate treatments and water was 202.25 and 47.58 g, respectively.

Scaphoideus titanus Ball, the main vector of Flavescence dorée phytoplasma, was monitored in Slovakia during 2013 and 2014. The species was present in the vineyards of eastern Slovakia but it was rarely found in the south of central part of the country. It was also found at many locations of western Slovakia but not in the southeast of western Slovakia. Higher abundance of S. titanus was detected in the vineyards with biological control. Varietal preference of the leafhopper was not confirmed. The first nymphs were found on June 5 and the last nymphs on July 17, adults occurred from the end of June to the end of September. There is one generation of S. titanus per year in Slovakia.

Long-term field experiments are important for assessing the yield response of crops to different tillage systems and pre-crops. An experiment was established in 1996 in Raasdorf (Austria) on a chernozem with four tillage treatments (mouldboard ploughing (MP), no-till (NT), deep conservation tillage and shallow conservation tillage) and two crop rotations. Winter wheat yields were generally at similar levels with all four tillage systems in most years between 1998 and 2012. Yields increased with higher amounts of rainfall during the vegetation period (from October until June) with the smallest increase among tillage treatments in NT. This indicates that MP can be superior to NT regarding yield at higher amounts of rainfall. Pre-crops considerably influenced winter wheat with higher yields after maize, soybean and winter wheat than after sugar beet. In one year with high rainfall, a tillage × pre-crop interaction showed that yields were lower after maize in NT than in other tillage systems whereas yields after sugar beet tended to be higher with NT in years with low rainfall.

The yield forming response of maize cultivar to zinc (Zn) application depends on its timing. This hypothesis was validated in 2007, 2008, 2009 and 2010 growing seasons. The zinc treatments as the first factor were: NPK; NPK + Zn applied before sowing; NPK + Zn applied to maize at the stage of 4^th leaf. The second factor was the maize type: stay-green (modern cultivars) - Paroli, Veritis, Anamur; classical (old cultivars) - Inagua, Kirola. The grain yield of modern cultivars responded the best to zinc applied before sowing, whereas the old ones, when applied to foliage. The yield of the stay-green maize depended upon the number of kernels per row, whereas the classical one on all yield structural components. The zinc management in the modern cultivars should be oriented towards maximization of the number of kernels per row, whereas in the old one on its optimization with the simultaneous kernel weight increase. The positive impact of zinc application before sowing on dry matter translocation from vegetative tissues to growing kernels underlines its practical usefulness, especially in areas with frequent water shortage during maize growth.

The structure of the dry material plant is made up of a cell tissues system. The walls of the cells are formed by cellulose and lignin, ingredients difficult to biodegrade. Therefore, the aim of the breeders performed a variety of energy plants, providing a high yield of dry matter in the green state. This applies particularly to herbs, grown on agricultural and arable land (for example cereals, maize, sorghum, hemp, amaranthus, mallow, sugar beet, potatoes, fodder crops on arable land, permanent grassland and other). The subject of the experiment was to evaluate selected varieties of maize and sorghum and to determine the suitability of the materials on the device model for anaerobic fermentation mixed with beef slurry, and ferment from the biogas plant. Among the varieties of maize there are the significant differences in the production of biogas in the same conditions. Of the three maize varieties (Fixxif, Saxxo, Benicia), the most powerful was Saxxo.

The J wave is a deflection immediately following the QRS complex of the surface ECG. The J wave has been observed in humans under physiological and pathophysiological conditions. We describe in this paper the ratio of incidence of this phenomenon in healthy dogs and dogs with pathological disease and the effect of exercise on its size and shape. At rest, a J wave was observed at the R-ST junction of the ECG in 11 of 34 adult dogs, usually in leads I, II, III, aVR, and aVF and left lateral precordial leads. After a submaximal exercise test there were no variations in the shape or the size of the J wave.

Canine aggression is a widespread problem which receives a lot of attention from the media. It has important consequences in terms of public health and animal welfare. To address this issue it is necessary to first determine its epidemiological characteristics in the target population. This study was aimed at exploring the characteristics and determining the risk factors for dog aggression in the Slovak Republic and, in particular, for aggression directed at humans. For this purpose, we used a questionnaire directed to dog owners which included information about dog and owner characteristics, housing, training and fear behaviour. From the 177 analysed questionnaires, 67% portrayed dogs with some type of aggressive behaviour. Half of the animals showed some degree of owner-directed aggression and almost 40% were aggressive towards unfamiliar people. The approach of an unfamiliar male was the situation that stimulated most dogs to behave aggressively (33.3%) and a small percentage (2.3%) of the animals always showed aggression when approached by an unfamiliar child. Multivariate logistic regression models found that "owner's age" (OR, 0.96; 95% CI, 0.93 - 0.99) and "neuter status" (OR, 0.36; 95% CI, 0.13-0.98) were significantly associated (P < 0.05) with the exhibiting of aggressive behaviour in different contexts. "Dog's sex" (OR, 2.16; 95% CI, 1.09-4.27) and "type of training" (OR, 2.32; 95% CI, 1.19-4.80) were significantly associated (P < 0.05) with the exhibiting of aggressive behaviour towards familiar people. Younger owners were more likely to have aggressive dogs and neutered dogs were less likely to be aggressive in different contexts. Male dogs and dogs with informal training were more likely to be aggressive towards familiar people. Manifestations of fear were significantly associated with the expression of aggressive behaviour in all models. This study provides the first preliminary results on the characteristics and potential risk factors for human-directed aggression in Slovakia. The analysis of these data may help in determining which preventive measures should be given priority in this country.

Despite some progresses in the political situation, the effects of the economic crisis and widespread food insecurity are expected to persist. Humanitarian assistance is now needed to support the needs of the most vulnerable. In the longer term, countries have the significant goal of consolidating peace and security and strengthening the overall governance, while at the same time, reconstructing and rehabilitating their economy.

The wheat stem rust pathogen, Puccinia graminis f.sp. tritici, is a significant and devastating disease of wheat crops worldwide. Wheat has many wild relatives in which to source new resistance genes, including the cereal crop of rye in the tertiary genepool. The aim of this study was to assess the reaction of 1BL.1RS and 2RL.2BS double wheat-rye translocation lines to virulent stem rust races from Africa and North America. BC₁F₃ and BC₁F₄ populations from a cross between the line KR99-139 (a double wheat-rye translocation line with 1BL.1RS and 2RL.2BS) and the bread wheat cultivar Topper were used in the study. Several of the populations homozygous for 1BL.1RS and heterozygous for 2RL.2BS showed resistance and low severity adult plant resistance (20RMR-50MSS) to the African stem rust race TTKSK in the field. None of the tested populations with varying chromosome combinations showed seedling resistance to any of the tested stem rust races. Thus, these resistant populations likely carry gene/s effective at the adult plant stage since all stage resistance genes with major effect appear to be absent based on the seedling assays. Resistant lines combined three chromosomes (1RS, 2RS and 2BS) which make their direct use in breeding more complicated. Mapping studies followed by potential transfer of genes between 2R and 2B will make the identified minor genes more useful in wheat breeding.

The present studies determined the antagonistic activity of selected fungi of the soil environment of root chicory -a high-inulin plant, with considerable pro-health values - towards such fungi pathogenic towards this plant as: Altenaria alternata, Botrytis cinerea, Fusarium culmorum, F. oxysporum, Thanatephorus cucumeris and Sclerotinia sclerotiorum. The cultivation of root chicory took into consideration soil mulching with cover crops (oats, tansy phacelia and common vetch) as well as the conventional cultivation, i.e. without any cover crops. The total population of fungi after soil mulching with common vetch was almost twice as small as in the control and smaller than with phacelia as a cover plant. Antagonistic Clonostachys spp., Myrothecium spp., Penicillium spp. and Trichoderma spp. displayed differentiated activity towards the studied fungi. The greatest antagonistic effect was observed after the mulch of oats. Besides, oats and common vetch the most positive effect on the antagonistic activity was that of Clonostachys spp., Myrothecium spp., Penicillium spp. and Trichoderma spp. Those fungi were most effective in inhibiting the growth and development of F. oxysporum, T. cucumeris and S. sclerotiorum.

In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. - two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage.

Aneurysmal bone cysts are expansile osteolytic bone lesions that can manifest clinically as lameness, pain and swelling. In humans, aneurysmal bone cysts are considered benign tumours that have been classified as primary or secondary to a previous coexisting lesion. Local recurrence after treatment or malignant transformation has been reported in both humans and in a dog. Different treatment options have been proposed. This report describes the treatment of an 11-month-old Irish wolfhound with an aneurysmal bone cyst with a combination of computed tomography-guided cementoplasty and radiation therapy, 30 Gy delivered in 10 fractions. The dog recovered completely, with no lameness observed 30 months after treatment.

The high prophylactic doses of ZnO commonly used to control post-weaning diarrhoea (PWD) in piglets have become an environmental problem. The possibility of partial replacement of ZnO by sodium humate (HNa) in PWD treatment was investigated in this study. Weaned piglets (32) were challenged with two enterotoxigenic E. coli strains (ETEC/O149/F4/LT and ETEC/O147/F18/LT) and allocated into four treatments maintained for three weeks: C (no supplementation); ZnO2.5 (2.5 g ZnO/kg diet); HNa + ZnO1.0; HNa + ZnO1.5; HNa + ZnO1.7 (20 g HNa and 1.0, 1.5 and 1.7 g ZnO/kg diet, respectively). The effects on incidence, severity and duration of diarrhoea, faecal shedding of total E. coli and both ETEC strains, growth rate and selected blood parameters were investigated. In contrast with ZnO2.5 and HNa + ZnO1.7, high daily diarrhoea scores, incidence and duration and mortality due to severe dehydration were seen in C, HNa + ZnO1.0 and HNa + ZnO1.5 groups. The administration of ZnO and HNa did not affect the faecal shedding of the challenged ETEC strains for eght days, even in clinically healthy piglets in ZnO2.5 and HNa + ZnO1.7 groups. Signs of growth depression were found in C; HNa + ZnO1.0 and HNa + ZnO1.5 groups during the first week. No difference in growth performance was observed in ZnO2.5 and HNa + ZnO1.7 piglets. Most of the selected biochemical and haematological parameters did not differ significantly among the treatments. However, a significantly higher serum Zn as a result of high dietary ZnO intake in the ZnO2.5 group compared to the control and HNa groups was detected. Significantly lower serum P in ZnO2.5; HNa + ZnO1.7 and HNa + ZnO1.0 groups compared to the control group was most likely induced by the increased Zn in serum. The results indicate the possibility of reducing the high pharmacological levels of ZnO in the prophylaxis of PWD through partial replacement with HNa. Such a treatment maintains the favourable prophylactic effect while lowering the Zn content in faeces.

We conducted to describe methods used to differentiate the zones of juvenile and mature wood in stems of coniferous species and to present the importance of good identification of the shares of both types of wood to ensure a rational use of the raw material and final quality of wood products. This study describes in more detail a novel method to separate the juvenile and mature wood tissue in stems of European larch using cluster analysis in the form of the k-means algorithm. Moreover, guidelines were also shortly described for forest management which could result in a reduction of the share of juvenile wood in stems of forest trees.

The concept of sustainable development is currently known and used in many contexts across a wide range of industries. Nevertheless, specific approaches to achieving the strategy of sustainability can be found in the individual sectors. The beginnings of sustainable management in forestry go back to the mid-18^th century. Currently, certification systems contribute to sustainable behaviour in forests. In the Czech Republic, this concerns in particular PEFC and FSC certification systems. Both these certification systems include two different processes, forest management certification and chain of custody certification, i.e. including the wood-processing companies, where only certified wood must strictly be used. This article aims to evaluate the importance of certification for sustainable development in forestry. Given the clear superiority of the PEFC system in the Czech Republic, this paper focuses on the potential benefits for PEFC certificate holders. To determine the benefits of PEFC certification for sustainable development, a questionnaire survey was chosen. Assessment of the benefits for sustainable development depends on the subjective assessment of certification holders. The results suggest that the beneficial effect on sustainable development in forest management is clear in the forest property area of over 500 ha.

A method for the rapid and nondestructive determination of total nitrogen content in soy sauce was explored. Prediction models were established using near-infrared spectroscopy combined with each of the following techniques: partial least squares (PLS), interval PLS, synergy interval PLS (siPLS), and backward interval PLS. Results showed that each improved forecast model was better than the PLS model. The siPLS method exhibited the best performance. The full spectrum of light soy sauce was divided into 20 subintervals. The combination of four subintervals, namely, 6, 11, 13, and 18, showed the best effect, with a correlation coefficient of 0.9977 and an RMSECV of 0.0198. The full spectrum of dark soy sauce was divided into 20 subintervals. The combination of three subintervals, namely, 14, 17, and 19, showed the best effect, with a correlation coefficient of 0.9818 and an RMSECV of 0.0640. Therefore, the siPLS method can realise a rapid and accurate detection of total nitrogen content in soy sauce.

Downy mildew [Pseudoperonospora cubensis (Berk. & Curt.) Rostov] is a major destructive disease of cucumber (Cucumis sativus L.) worldwide. Resistant cultivars were recently introduced into Egypt, but yield losses were high if no fungicides were used. The objective of this study was to identify sources of resistance to downy mildew among Plant Introduction (PI) cucumber accessions from the U.S. National Plant Germplasm System. We evaluated 133 such accessions for downy mildew resistance under natural field epidemics during the summer 2013 at El-Beheira Governorate, Egypt. Mean ratings for downy mildew leaf damage ranged from 1 to 9 on a 0 to 9 scale. We classified 18 PI accessions (13.5%) as highly resistant (rating of 1.0-2.0), 46 (34.5%) as moderately resistant (rating 2.1-4.0), 40 (30%) as intermediate (rating 4.1-6.0), 12 (9%) as moderately susceptible (rating 6.1-7), and 17 (13%) as highly susceptible (rating > 7.1). The most resistant PI accessions were PI 432870, PI 432873, PI 432878, PI 432884, and PI 432886 with a rating of 1, which originated from China. The most susceptible PI accessions were Ames7736, PI 211979, PI 288991, PI 288992 and PI 289698 with a rating of 9. The five most resistant and five most susceptible accessions were further evaluated in replicated experiments during the summer 2014. Results from the repeated test confirmed the results from the first screening. No PI accession was found immune to downy mildew. However, high levels of resistance were observed in several PI accessions that could be useful for the breeding for resistance to P. cubensis in cucumber.

Winter Orychophragmus violaceus (OV)/spring maize is a novel eco-agricultural system in North China Plain, but little is known about OV's nitrogen (N) effects on succeeding maize growth and its contribution to maize N uptake and utilization during the growth. A pot experiment using ¹⁵N was conducted, including five treatments: (1) Control - without OV incorporation and N fertilizer; (2) G - high ¹⁵N labelled-OV incorporation; (3) 50%G - lower ¹⁵N labelled-OV incorporation; (4) F - ¹⁵N labelled urea fertilizer merely; (5) G + F - high OV incorporation combined with urea fertilizer. Increasing OV incorporation rate increased maize dry matter, yield, and total N uptake. Approximately 84-97% of the released OV's nitrogen was absorbed before the V8 (the 8^th leaf fully expanded) stage in G and 50%G treatments. However, only 19% of the released OV's nitrogen was taken up at this period in G + F, and the rest was absorbed from V8 to maturity. G treated maize doubled the OV's nitrogen uptake than the 50%G at maturity, and also had higher percentage contribution of OV's N to total N accumulation. The G and 50%G maize obtained similar OV's nitrogen use efficiency of 30%; however, this efficiency was further improved to 43% in the G + F. And more, G + F treatment further improved the maize yield by 9% compared to F treatments.

The field experiment with different soil tillage treatments has been carried out in Prague-Ruzyně locality (Czech Republic) since 1995. Data of two growing cycles in the years 2007-2010 and 2011-2014 were evaluated. Tillage technique was decisive for changes in soil characteristics and crop yields. Bulk density, organic carbon (C_org) and microbial biomass C (C_mic) were more equilibrated throughout all tested soil layers (0-0.1; 0.1-0.2 and 0.2-0.3 m) in conventional tillage (CT). In reduced tillage (RT) and no-tillage (NT) treatments significant accumulation of C_org and increase of C_mic in the surface layer were found, compared to CT. No significant differences in C_org and C_mic between two growing cycles were determined; however, mostly higher values were obtained in the top layer of NT during the second growing period. Higher bulk density under conservation tillage techniques did not negatively affect soil characteristics and should be taken in consideration for data evaluation as it can alter interpretation of their changes in the soil profile. Crop yields were comparable in CT and RT. Yield decrease in NT was mostly observed for winter wheat and pea. Beneficial effects of RT and NT conserving soil moisture on crop yield were not observed in dry years.

Autotransplantation of teeth is the most natural technique to for replacing missing teeth in exposed parts of the dental arch. Reports from human patients indicate great progress toward successful transplantations. However, complications such as inflammation and ankylosis still occur. To understand regenerative processes after autotransplantations, several animal models have been used (monkeys, rodents, rabbits, cats and dogs) and histological/molecular methods have been established. This review aims to summarise knowledge from animal models and discuss their advantages or disadvantages with respect to possible usage in research.

In this work we compared two species of fish with different feeding habits: Flathead mullet (Mugil cephalus) and Gilthead sea bream (Sparus aurata). The aim of this study was to evaluate total oxidant status (TOS), total antioxidant capacity (TAC) and TOS/TAC ratio (OSI), in order to highlight the presence of any differences and correlations in these two different species of fish. Thirty adult fish of Mugil cephalus and thirty of Sparus aurata were used. From each fish 0.6 ml of blood was collected. TOS and TAC indicators were measured in serum obtained from samples previously clotted and centrifuged. Our results showed statistically significant differences between the two species in TAC. TOS and OSI did not show significant differences between Gilthead sea bream and Flathead mullet. A positive relationship between TOS and TAC was found in Flathead mullet (Mugil cephalus), and a negative relation between TOS and TAC in Gilthead sea bream (Sparus aurata). Our study indicates that the oxidative status and the relationship between total oxidant status (TOS) and total antioxidant capacity (TAC) in serum are probably dependent on the fish species and are affected by different feeding habits.

Sustainable food consumption is a core policy objective of the new millennium in the national and international agenda, as a consequence, the attention towards sustainable consumption has significantly increased in the last decade. However, the consumer attitudes and buying behaviours of sustainable food are still not completely understood. In this context, this paper aims to investigate the factors affecting consumers' attitudes towards food products with sustainable attributes. The first part of the work presents an overview of the theoretical concepts of sustainable consumption. Subsequently, the results of an empirical analysis with a sample of 300 consumers aimed at assessing factors' affecting consumer attitudes towards food products with sustainable attributes, such as Organic, Fair Trade and typical products, are presented. The results presented in this paper are valuable for both producers in the formulation of marketing strategies and for public institutions in the planning of the programs of education and information for the promotion of sustainable consumption.

The contents of nitrogen (N), potassium, phosphorus, calcium, magnesium and sodium were estimated in tubers of Jerusalem artichoke Helianthus tuberosus coming from the field experiment conducted over 2010-2012. The experimental factors were the cultivars of Jerusalem artichoke cvs. Albik and Rubik and different nitrogen fertilization levels, against phosphorus and potassium fertilization and the full dose of manure. Determination of elements in the soil and the dry weight of the aerial parts are performed by standard methods. When using a fixed level of phosphorous-potassium fertilizer, the highest sodium content was obtained at a level of 50 kg N/ha, magnesium and sulphur at a dose of 100 kg of N, nitrogen - 150 kg N/ha, potassium and calcium - in the objects of fertilizer phosphorus-potassium, and phosphorus - in the building control without fertilization. Cv. Albik proved to be more abundant in mineral elements than cv. Rubik. The latter was characterized by a higher stability of characteristics.

This study evaluated the effects of biochar application on some properties of an alkaline soil and on lentil (Lens culinaris Medik) growth. Lentils were grown in the soil amended with the rates of 0.4, 0.8, 1.6, 2.4, and 3.3 weight percent of two biochars (B₁ and B₂), produced from rice husk under different pyrolysis conditions. Lentils were harvested after 70 days. Soil samples were also analysed for changes in physico-chemical properties. The results indicated that biochar application significantly increased soil organic carbon, cation exchange capacity, available potassium and below ground biomass of lentil, while it decreased soil bulk density. The results suggested that biochar application to alkaline soils has benefits to both soil quality and plant growth.

Polymorphic SNPs were identified using BovineSNP50 BeadChip in three groups of cervids: farmed Red deer (n = 3), and free range Red deer (n = 5) and Fallow deer (n = 2). From the total of 54 609 SNPs, 53.85% could be genotyped. Out of 28 502 successfully genotyped autosomal SNPs only 5.3% were polymorphic. The average minor allele frequency within cervids was 0.23 (number of polymorphic SNPs ranged from 467 to 686). Results of the molecular variance analysis showed that 67.38% of variation occurred within individuals and the rest was explained by a species difference (F_ST = 0.32). The value of F_IT (0.33) indicated a higher proportion of homozygote genotypes in the analyzed dataset. Pairwise F_ST values showed very clearly the genetic differentiation between Red and Fallow deer which ranged from 0.06 (farmed and free range deer) to 0.74 (farmed Red and Fallow deer). A similar result was found for Nei's genetic distances that ranged from 0.01 (among Fallow deer) to 0.79 (among farmed Red and Fallow deer). The genetic differentiation of the analyzed cervid species was evaluated also by the principal component analysis with the involvement of 6 other species from the family Cervidae, which showed a division of the Cervidae cluster into 7 subpopulations. The panels of SNPs primarily produced for a model species are becoming the marker of choice for the application in other species, but the best methods of their discovery, validation, and genotyping in non-model species need further investigations.

The objective of this work is the evaluation of threats in the field of safety, fire safety, quality and environment in the post-harvest treatment of grain, with an emphasis put on pre-cleaning and drying of grain. The paper describes the technology of pre-cleaning and drying of grain. Risks for individual machines were evaluated using a failure mode and effects analysis (FMEA), which is used to evaluate the threats in terms of fire risk, occupational safety, the effect of machine on the quality of final product, and effect on the environment. We have proposed measures and solutions to eliminate or minimise the consequences of threats. Risk rates are evaluated before and after measures adopted. It can be stated based on the results that risk was successfully reduced to an acceptable level after adopting measures.

The interactions of different organic acids such as citric, malic, oxalic, and fulvic with aluminium were studied using ion-exchange chromatography (IC) and high performance liquid chromatography (HPLC). The experiments were carried out at low pH (1.5-3.1). The results of IC experiments on the interaction between Al and oxalate, citrate, and malate were compared with model chemical equilibrium calculations. The strongest effect on Al speciation was observed with oxalic acid and fulvic acid. These two ligands formed more than one type of complex with Al. In contrast, there was no significant effect of malic acid on Al speciation and a rather weak effect caused by citric acid. The studies show that the formed complexes are stable even at low pH.