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Electroretinography (ERG) in the form of full-field, flash ERG is the most commonly used technique in veterinary ophthalmology for diagnosing the functioning of the outer retina. Under light stimulation spatially distributed different cell types within the retina produce time-varying electric responses. These are recorded in the form of ERG traces consisting of a series of positive and negative wavelets. The possibility of selective stimulation of individual types of retinal cells and the analysis of constituent components of ERGs are the basis for determining the source of abnormalities and diagnosis of various types of dysfunction. In many cases, the ERG allows diagnosis of hereditary retinal disorders in dogs before the appearance of behavioural and ophthalmoscopic symptoms. This review is an introduction to the electrophysiology of vision, intended for small animal practitioners, and aimed at presenting the benefits of ERG for early ophthalmic diagnostics in dogs.

Forty six Holstein Friesian repeat breeding cows (the average AI/conception was 5.2 ± 0.2) were investigated using the progesterone assay after AI to determine possible differences in plasma progesterone profiles between summer and winter seasons. Twenty eight (60.9%) and 18 (39.1%) cows were followed in summer (June-August) and winter (December-February), respectively. In the summer season, the total progesterone concentrations were higher (P < 0.05) in pregnant cows with normal luteal function compared to those in non-pregnant animals with abnormal luteal function. In contrast, in the winter season, there was no difference (P = 0.12) in total progesterone concentrations between pregnant and non-pregnant cows with normal or abnormal luteal functions. When the progesterone concentrations were compared, the pregnant and non-pregnant cows with normal luteal functions exhibited no difference (P = 0.92) in summer and winter seasons. Thus, the present study indicates that there is no effect of season on plasma progesterone profiles in repeat breeding cows; however in the summer season, the total progesterone concentrations were considerably higher in pregnant cows with normal luteal function compared to non-pregnant cows with abnormal luteal function.

Acute colic in horses, especially presentations requiring surgical correction, such as large colon volvulus, very often involves defective or excess platelet activation. The PFA-100 is a new point-of-care analyser that evaluates platelet function by measuring closure time (CT) in the whole blood of healthy horses in a standardised manner. However, there are no reports on platelet function in horses with colic measured by the PFA-100. The aim of the present study was to investigate platelet function in a group of horses suffering ascending colon displacement (ACD) and who underwent surgery compared to a group of control (C) horses. Thirty ACD cases and twenty untreated clinically healthy control horses were included. Blood samples were collected from the ACD horses prior to and 24, 48 and 72 h after laparotomy. Red blood cell count (RBC), haematocrit (HCT), haemoglobin (Hb) concentration, white blood cell count (WBC), total plasma protein (TP) concentration, platelet count (PLT), platelet volume (MPV) and ADP closure time (CT-ADP) were measured. CT was determined on a PFA-100® device using collagen/ADP cartridges. There were no significant differences in RBC, PLT and MPV between C and ACD horses pre-operatively. Reduced PLT was observed in the post-operative period. The mean CT-ADP of C horses was 96.43 ± 12.53 and 91.43 ± 2.51 seconds in the ACD group prior to surgery. CT-ADP was changed after surgery whereby CT-ADP time was more than doubled. We conclude that the PFA-100^® can be used to detect platelet function defects in horses.

The cooperative movement in Poland has a long but difficult history, which has caused farmers to have an aversion to cooperatives. Nonetheless, in the early 1990s, the first farmers' cooperative marketing organizations, which were called agricultural producer groups, appeared in the market. These groups are bottom-up, voluntary organizations the primary purpose of which is to jointly sell their members' output. In this paper, it is investigated why the new forms of governance, namely cooperative arrangements, were chosen, and we evaluate the implications of these choices on the market success of these groups. Empirical data were collected from 62 producer groups in one Polish province. We found that the groups were typically functioning as associations, unions, and limited liability companies. The factors that had an impact on the choices made were the number of members and the specific investment per member. Additionally, if the initial investment level was low, not only set-up and operational costs but also tax considerations played a role in the decision. Therefore, we argue that the new bottom-up cooperatives, which are theoretically suitable when the start-up capital is high or the number of members is large, will gradually be recognized and accepted in the market despite the fact that these cooperatives have a "bad reputation" caused by the socialist legacy. The new cooperative development trend confirms this argument.

Potential application of a metal oxide semiconductor based electronic nose (e-nose) as a non-destructive instrument for monitoring the change in volatile production of banana during the ripening process was studied. The proposed e-nose does not need any advanced or expensive laboratory equipment and proved to be reliable in recording meaningful differences between ripening stages. Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), Soft Independent Modelling of Class Analogy (SIMCA) and Support Vector Machines (SVM) techniques were used for this purpose. Results showed that the proposed e-nose can distinguish between different ripening stages. The e-nose was able to detect a clear difference in the aroma fingerprint of banana when using SVM analysis compared with PCA and LDA, SIMCA analysis. Using SVM analysis, it was possible to differentiate and to classify the different banana ripening stages, and this method was able to classify 98.66% of the total samples in each respective group. Sensor array capabilities in the classification of ripening stages using loading analysis and SVM and SIMCA were also investigated, which leads to develop the application of a specific e-nose system by applying the most effective sensors or ignoring the redundant sensors.

One of the aims in red clover (Trifolium pratense) breeding is to increase the polyphenol oxidase (PPO) activity, which may effectively reduce protein breakdown in silage and when cattle are fed by fresh clover. We analysed total PPO activity spectrophotometrically and on the level of gene expression using real-time quantitative PCR in single plants derived from an interspecific T. pratense × T. medium hybrid. Experiments were performed for two years and evaluated according to the general linear model with three factors (family, year, and cut). The analysis revealed considerable variability in total PPO activity between individuals and between families. Four families and two individuals with significantly higher PPO activity were selected. Their PPO activity ranged from 3.411 to 3.547 mkatal/min/g and from 4.041 to 5.731 mkatal/min/g, respectively, in comparison with the control variety Amos (2.370 mkatal/min/g). The majority of PPO transcripts were expressed by the two genes PPO1/5 and PPO2. In some genotypes, the PPO5 gene was expressed. Quantitative PCR confirmed the highest activity of PPO genes in seven hybrid plants with higher DNA contents corresponding to 30 chromosomes with 815 013 copies per plant. Our results indicate the suitability of combining two methods for improved selection: initial expression analysis to assess the PPO transcript level indicating gene activity and subsequent enzymatic assay.

Cereal/legume intercropping may improve resource use efficiency in agroecosystems and increase yield per unit surface area and yield stability. Two field bean (Vicia faba L.) and four barley (Hordeum vulgare L.) cultivars were mono- and intercropped (additive design) in a 2-year lysimeter experiment on a sandy loam soil. The aim was to test the effect of the cropping system on dry matter and N yield of forage, the residual effect on the subsequent ryegrass crop (Lolium multiflorum Lam. westerwoldicum), and NO₃-N leaching in the rotation. Land equivalent ratios were 1.65 for dry matter and 1.67 for N yield, indicating a clear advantage of the intercrop over sole crops. Both species suffered from competition, especially in terms of N resources, but barley was less affected. Nitrate leaching was the lowest from intercrop. Preceding crop significantly affected dry matter, N content and NO₃-N leaching of ryegrass. Field bean sole crop gave the highest benefits to ryegrass in terms of forage dry matter and N content, but also the highest NO₃-N leaching, followed by the intercrop and the barley sole crop. Barley/field bean intercropping may be an effective strategy to reduce land requirements, N leaching losses and fertilizer inputs, thereby increasing the sustainability of farming systems.

The aim of the research conducted on eutric brown soil on the experimental facility of Faculty of Agriculture, University of Zagreb in 2012 and 2013, was to determine the optimal sowing density for two cultivars of white lupin (Teodora and Energy) and a cultivar of narrow-leafed lupin (Arabella). The study included three sowing densities: 60, 75 and 90 germinated seeds/m². The cultivars of white lupin achieved significantly higher seed yield, higher 1000 seed weight, higher weight of seeds per plant and higher protein content in the seed, compared to the cultivar of narrow-leafed lupin. The sowing density had no effect on seed yield, but the increase in sowing density above 60 germinated seeds/m² significantly reduced the number of pods, the number of seeds and seed weight per plant.

Phosphorus (P) is essential for crop production. Adequate application P rate is critical for enhancing productivity and profitability of snap bean (Phaseolus vulgaris L.). The goal of this study was to optimize P application rate for commercial snap bean production in south Florida. Six trials were conducted on sandy soils and muck soils in Hendry County and Palm Beach County, Florida, USA. Before planting cv. Caprice snap bean, plots were fertilized with different P application rates in the form of triple superphosphate (0-45-0). An increase in P₂O₅ application rates up to 134 kg/ha P₂O₅ significantly increased the marketable bean yields. However, beyond that point, significant field gains did not occur with further increased application. At 134 kg/ha P₂O₅, bean appearance quality was the best compared to the other treatments. These results indicate that 134 kg/ha P₂O₅ produced best bean appearance quality and was the optimum rate for commercial production of snap bean in high-pH soils in south Florida.

The aim of this investigation was the analysis of the variability within the internal and external ophthalmic artery in the chinchilla (Chinchilla laniger, Molina). The head vasculature of 65 individuals was analysed, with particular emphasis on the internal ophthalmic artery originating from the central and rostral part of the cerebral arterial circle. Head blood vessels were filled with acrylic latex for vascular corrosion casting. The results showed ten variants of blood supply for the orbit, with a predominance of the first variant (66.1%) = bilateral presence of the external ophthalmic artery originating from the maxillary artery. Other variants differed in symmetry and asymmetry, sites of origination and the coexistence of both internal and external arteries. Vascularisation of the brain in chinchillas originates mainly from the vertebra-basilar system. The observed variability seems to confirm the role of the basilar artery in the arterial blood supply of the brain in this species.

The objective of this study was to determine the effect of canine inflammatory bowel disease (IBD) on the density of cocaine- and amphetamine-regulated transcript (CART) immunoreactive nerve fibres in the mucosa of different sections of the gastrointestinal tract. Fibre density was analysed in mucosal specimens from the duodenum, jejunum and descending colon of healthy dogs and patients with inflammatory bowel disease of varying intensity. Nervous fibres were stained in single-cell immunofluorescence assays. The density of CART-immunoreactive fibres was determined using a semi-quantitative approach by counting the number of fibres in the field of view (0.1 mm²). An increase in the density of CART-immunoreactive fibres was observed in dogs with moderate and severe IBD in comparison with healthy subjects. The results suggest that the presence of CART in enteric nerve fibres could play a role in the pathogenesis and development of canine IBD.

Physical properties of soil can be affected by machines that are used for skidding which have the potential to impact soil sustainability and forest productivity. The present study evaluated the effect of timber skidding by a rubber-tired skidder and farm tractor on the soil physical properties. The study site was located in Hafdaghanan plantation region in the north of Iran. Two treatments that consisted of skidding by HSM 904 skidder and ITM285 farm tractor equipped with trailer were applied. The trails were included in three traffic levels: primary, secondary and tertiary. Treatment effects were tested using analysis of variance (ANOVA) with skidding machine types and traffic levels. Results indicated that machine type and traffic intensity are the effective factors on soil physical properties. The results showed that with an increase of traffic frequency, bulk density increased but total porosity and soil water content decreased. The results of the study provide clear evidence that farm tractor is a more environmentally friendly machine than rubber-tired skidder in the plantation area.

The fungus Fusarium oxysporum f.sp. pisi (FOP), the pea wilt pathogen, causes appreciable yield losses under favourable environmental conditions in Algeria. Studies on the pathogen variability and distribution of races are essential to identify effective sources of resistance to this disease. In this study, a survey was conducted during the period 2007-2011 in four different agro-climatic zones. Pathogenic variability in 52 isolates of FOP, collected from different pea-growing areas of western Algeria, were evaluated using 7 genotypes as differential hosts. Results indicated that the disease was prevalent in all fields prospected and isolates obtained were assigned to Races 1, 2, 5 or 6 by their pathogenicity. It was found out that Races 1 and 2 were more common in all areas with 61.5 and 19.2%, respectively. This study is the first report of pea Fusarium wilt races distribution in Algeria.

The quantitative and qualitative accuracy of the routine histological method for the determination of unauthorised animal and herbal content in minced meat was to evaluated. Laboratory adulterated minced beef meat; each containing 5, 10, 15 and 20% of soya and chicken gizzard was prepared. Then each sample was divided into three parts and four paraffin embedded blocks were prepared from each part. The sections were stained using haematoxylin and eosin, toluidine blue and Masson's trichrome. The histological examination revealed the soya and gizzard tissues clearly in all the samples. The histometrical analysis showed that there was no significant difference between the estimated percentages of both additive tissues and the real related percentages. Overall, neither was there any significant difference between the data of the three parts of each sample and the real percentages. The findings of the present research suggest the histological technique as an effective method for qualitative and quantitative evaluations of minced meat.

The wheat cultivar Weimai 8 is a good source of adult-plant resistance to leaf rust in China. In order to understand the genetic background of the resistance genes, 179 F2:3 families derived from the cross Weimai 8 and Zhengzhou 5389 (susceptible to leaf rust) were used to construct a linkage map. Results showed that there was a major QTL on chromosome 2AS, temporarily named QLr.hbau-2AS, responsible for the resistance. QLr.hbau-2AS from the resistant parent Weimai 8 was between the SSR markers Xcfd36 and Xbarc1138, with an interval length of 2.58 cM. In the 2010-2011, 2011-2012 and 2012-2013 crop seasons, it explained 25.79, 71.55 and 60.72% of the phenotypic variation, respectively. The location of QLr.hbau-2AS was close to the leaf rust resistance gene Lr37. However, they may not be the same since QLr.hbau-2AS has a different virulence test response from Lr37. This study identified two closely linked flanking molecular markers QLr.hbau-2AS and these molecular markers will help marker-assisted selection in breeding resistant cultivars in the future.

The long-term effect of 87.3 kg/ha P on the yield elements and nutrient content of maize was studied at the National Long-Term Fertilization Experiment of the Karcag Research Institute in Hungary. The soil of the experiment site is non-calcareous Luvic Phaeosem, and its soluble phosphorus (P) and zinc (Zn) content in 0-20 cm soil layer are: ammonium lactate P: 141.1 mg/kg and diethylene triamine pentaacetic acid (DTPA)Zn: 0.85 mg/kg, respectively. The effect of foliar Zn fertilization was studied at three levels of nitrogen (150, 200 and 250 kg/ha) and under 87.3 kg/ha P and 82.6 kg/ha K application in four replications. The applied Zn amount was 700 g/ha. We measured the grain yield and the thousand-kernel weight. Leaf and grain samples were analyzed for phosphorus, zinc, potassium, calcium, magnesium and manganese content. Foliar Zn application did not increase the yield significantly, but it enhanced the thousand-kernel weight. The element content did not change significantly - neither in leaves nor in kernels. Under the examined habitat circumstances even the long-term application of 87.3 kg/ha P dosage did not cause Zn deficiency to such an extent which would lead to significant yield depression of maize.

This study was aimed to determine the impact of risk element content on the prevalence of the selected microbial groups in industrial soils of one of the steelworks in Poland. The concentration of heavy metals: Cd, Cr, Cu, Hg, Fe, Ni, Pb and Zn along with soil pH and the number of mesophilic bacteria, fungi, actinomycetes and Azotobacter was assessed in 20 soil samples. The limit concentrations of Cd, Pb and Zn were exceeded in five sites. However, even in these strongly contaminates sites, the studied microorganisms were abundant. The correlation between the concentration of heavy metals and microbial numbers was very weak and statistically insignificant. High numbers of fungi were observed in the contaminated sites, which was particularly interesting in one of the sites, strongly contaminated with Cd, Pb and Zn.

Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first tested with positive and negative control DNA and its sensitivity and specificity were determined. A new polymerase chain reaction protocol was developed by combining the new primer pair sets with bovine (12SM-FW and 12SBT-REV2) and ruminant host-specific primer sets (12S-FW and 12S-REV). In conclusion, the developed polymerase chain reaction assays can potentially be used for direct detection of Chlamydophila abortus in bovine and ruminant samples.

Spruces (Picea spp.) are considered to be the primary host trees of the double-spined bark beetle Ips duplicatus (Sahlberg, 1836), but occurrences on pines (Pinus spp.) are often reported. This paper documents the first recorded successful development of I. duplicatus on Douglas fir (Pseudotsuga menziesii) (Mirbel) Franco. Two densely infested Douglas fir trees were found in a forest dominated by Norway spruce (Picea abies) (L.) H. Karst., close to the village of Valšovice (Czech Republic). Ips duplicatus is an oligophagous species that attacks different coniferous tree species but was not previously reported to develop on Douglas fir. The infestation of Douglas fir recorded in the present study was most likely promoted by a large population of I. duplicatus in the studied region combined with a local lack of primary host trees as a consequence of bark beetle outbreak.

The comprehensive risk analysis of a business such as farming entails questions on what is at stake, how important is the risk concern and how to deal with it. We performed a sequential mixed method, with the in-depth interviews in the first stage (n = 35), followed by a survey on the Flemish FADN (n = 614) in the second, to investigate the farmers' risk perception, the attitudes towards risk and the perceived usefulness of the risk management strategies. We find that, rather than the short-term volatility in prices, the longer term co-evolution of expenses versus receipts is of a major concern to farmers, next to the land availability and the policy risks. Farmers are shown to be only slightly risk averse, rather risk neutral even. Further, our results suggest that farmers do not consider extensively studied risk management strategies such as contracts, futures and insurances, a valid option for their farm, and put more faith in internal strategies such as the debt management, the liquidity management and diversification. Last, risk management is to a substantial degree performed at the household level, rather than at the farm level, with strategies such as cutting the private expenses and the off-farm employment. These results hardly differ according to the farm and farmer characteristics.

The occurrence of chestnut blight (Cryphonectria parasitica) was studied in 2011-2012 at 13 locations in the main chestnut growing areas of Romania. Infections were detected at four localities. The symptoms and the fungus were detected on European chestnut (four localities) and also on oak trees (two localities). A total of 89 isolates of C. parasitica were isolated and characterised. Based on canker and isolate morphology (culture morphology and the Bavendamm test), both virulent and hypovirulent samples were isolated; hypovirulent isolates were found at only one locality. Two vegetative compatibility types corresponding to EU-12 and EU-2 were identified among isolates. Both mating types were found, with a dominance of MAT-1 in southern Romania and MAT-2 in northern Romania.

We investigate the effects of consumption of MRPs from the glucose-lysine model system heated 15°C-90 min on protein digestibility and its utilisation in young (3-week) and adult (12-week) rats. Nitrogen faecal excretion significantly increased after MRP consumption, especially during the third week. Protein digestibility was lower in both age groups studied, but the utilisation was unaffected. Parallelly, the nitrogen retention and its net utilisation for the entire experimental period did not vary. In young rats the faecal nitrogen exceeded the amount of ingested nitrogen coming from MRPs, suggesting that digestibility of undamaged nitrogen was affected. The same action is suspected in adult animals, but the results were not quantitatively conclusive, and therefore the effect should be moderate in this period.

The water of the breads was replaced with phenolic aqueous extracts from chicory, cabbage, celery, fennel, olive leaf, or grape marc wastes obtained through microwave-assisted extraction. The highest phenolic concentrations were found in the grape marc and in the bread enriched with it. The highest antioxidant activity values were measured in the crust of bread produced with the grape marc extract and in the crumb of bread with olive leaf extract. Generally, the replacement of water caused significant decreases of the specific volume, the shift of the crumb colour towards redder and yellower tones, and modification of gustatory and tactile attributes.

Three-year strict experiments with winter rapeseed were conducted in three experimental stations. In the research, winter rape fertilization with elemental sulphur (S) in the doses: 20, 40 and 60 kg S/ha, boron (B) - in the dose of 2 kg B/ha and copper (Cu) - 5 kg Cu/ha were applied. Microelements were introduced separately, in treatments B and Cu, as well as in combination B + Cu. The experimental soils featured low content of sulphate sulphur SO₄^2--S and boron, medium and low copper content. Rapeseed fertilization with sulphur, boron and copper resulted in their increased concentration in plants. Sulphur at 40 and 60 kg S/ha doses affected the increase in oilseed rape grain yield by 11-12% compared to the not fertilized treatment. The dose of 20 kg S/ha did not show any significant influence on yield. The yield increased by more than 10% in the comparison to the treatment without B and Cu, was obtained due to boron, as well as combination of boron and copper fertilization. A significant increase in fat content, in relation to not fertilized treatment, ranking from 1.0-1.4% dry matter, was recorded after fertilization with the highest sulphur dose - 60 kg S/ha, as well as after application of boron and copper fertilization.

This study compares the effect of application of digestate, straw, cattle slurry and inorganic fertilizers on crop yield and soil organic matter content. Total organic carbon (C), total organic nitrogen (N), hot water soluble C, microbial biomass C and hydrophobic soil components were evaluated in soil from the field experiment in Prague-Ruzyně (Orthic Luvisol, clay loam) with winter wheat. All fertilized treatments significantly increased grain yield above the level of non-fertilized control (5.68 t/ha), and the sequence was as follows: digestate (9.88 t/ha) > NPK(9.80 t/ha) > cattle slurry (9.73 t/ha) > digestate + straw (9.35 t/ha). Average organic C content in the soil ranged from 1.668-1.704% and the effect of different fertilization was not significant. The highest increase of microbial biomass C was recorded in digestate + straw (43.2% increase compared to control). Highly significant correlations were found between hydrophobic soil components and hot water soluble C (r = 0.988; P ≥ 0.05) and microbial biomass C (r = 0.964; P ≥ 0.05). Total organic N content ranged from 0.157-0.160% and differences among treatments were insignificant. Fertilization with digestate itself brings an effect in increasing crop yield, but does not improve the level of soil organic matter significantly.

Intensive tilapia egg incubation techniques create favourable conditions for microbial proliferation which often leads to massive mortalities of fish larvae. The effects of Nile tilapia (Oreochromis niloticus) eggs exposure to bronopol on decontamination of their bacterial surface and survival of larvae were observed. Immersion treatments of fertilized eggs were applied at 10, 25, 50, 100, and 250 mg/l of bronopol for 10, 20, and 30 min. This treatment substantially reduced the number of bacteria on Nile tilapia eggs. The greatest reduction in bacterial numbers (1.58 × 10³ colony forming units/g of egg) was observed at the maximum treatment dosage, i.e. 250 mg/l for 30 min, but this was not significant when compared with treatments of 100-250 mg/l bronopol for 10-30 min. Treatments of 50-250 mg/l bronopol provided better larval survival (89.33-94.67%) than those of < 50 mg/l (P < 0.05). The larval survival obtained from the Nile tilapia eggs disinfected with 10 and 25 mg/l bronopol for 10-30 min was similar to that of negative controls (79.33-80.97%). Additional in vitro test was performed to determine the inhibitory potency of bronopol against bacteria cultured from fertilized Nile tilapia eggs. Twenty-two bacterial isolates (Aeromonas hydrophila (n = 12), Staphylococcus aureus (n = 6), Escherichia coli (n = 2), and Micrococcus spp. (n = 2)) responded to minimum inhibitory concentrations of bronopol ranging from 64 to 128 µg/ml. The study demonstrates that the immersion treatment of Nile tilapia fertilized eggs with 250 mg/l for 10-30 min, the concentration proven effective against bacteria cultured from fertilized eggs, significantly reduced bacterial load and improved larval survival.

The impact of material solution of cover shells on stable daylight. Light is supplied to the stable via a roof skylight, where wired glass is the infill of skylight structure, through open side walls, whereby the influx of light on one side is influenced by the shelter, and through the open gates, which are located in the front walls of the stable were assessed. Measurements of light intensity inside the stable were carried out in two height levels. Simultaneously, measurements were performed outside, on non-shadowed plane. Daylight factor was calculated from the measured values of internal and external comparative illuminance. Resulting values were processed into tables and graphs. The roof skylight has clearly the greatest influence on the lighting of the stable. Its disadvantage is that the stable overheats in these places in summer. Here, it would be appropriate to replace the fill of the skylight with translucent light elements.

The authors present results of long term monitoring of beech stems bark necrotic disease of maternal stand in Czech Republic, Slovakia, Poland, Hungary, Romania, Serbia and Bulgaria. Overall, 10,863 beech trees were evaluated at 121 localities. The most trees (6679) were evaluated at 55 localities in Slovakia and 2684 trees at 50 localities in Bulgaria. In each country, we noted a wide interval of values of the beech stems necrotization index (I_SN) - e.g. in Bulgaria, from 0.22 to 1.50 and in Slovakia from 0.53 to 1.97. The average value I_SN in Slovakia (1.22) was out of all countries surpassed only by a value I_SN found out in Czech Republic (1.35). Overall, in Bulgaria, we registered more favourable state of beech bark necrotic disease than in Slovakia. As much as 80% of Bulgarian localities had values I_SN < 1.1 compared to only 49.1% of Slovakian localities. At the same time, 12.7% of Slovakian localities had values I_SN > 1.5, whilst there was no Bulgarian locality recorded in this interval. We consider Slovakia and Bulgaria the countries where the issue of the beech bark necrotic disease of tracheomycotic type is relatively well processed.

The contribution is focused on the food problem in the least developed countries, on the chosen areas where the overall situation is the most problematic. It deals with Burundi, belonging to the low income food deficit countries with one of the world's lowest rates of the gross domestic product per capita. The paper defines the food security situation in the global connection, representing a wide complex of economic, social, demographic, technologic and political aspects of production, distribution, shift and consumption of foodstuffs. The inter-related causes of food insecurity are mainly the long lasting civil wars, a limited access to land, environmental degradation, climatic shocks and the rapid population growth resulting from the high birth rates and the return of refugees. Subsistence crops and livestock products represent the main source of income for most households. The performance of these subsectors is very low, and generates chronic food deficits. Agriculture is thus the key sector in the predominantly rural economies and there is still a significant room for growth, diversification, increasing productivity and improving competitiveness.

Field validation of spot-fumigation with phosphine (PH₃) applied by a subcontracted pest-control company in a farm grain store infested by pests was conducted. Inside and outside of the fumigated grain spot, containers with adults of 5 species of coleopteran stored-product pests were regularly spaced. The beetle pests were the internally feeding Sitophilus granarius and Rhyzopertha dominica, and the externally feeding Tribolium castaneum, Oryzaephilus surinamensis, and Cryptolestes ferrugineus. A 100% mortality of all pest species inside the fumigated spot under the sheet used for the application was stated. The efficacy (maximal) sharply declined with the increasing distance from the fumigated spot: 50% mortality was observed at 5 m from the spot, 38% mortality at 10 m, and a mortality of 23% was observed at 15 m. Mortality was different among the species, and the most sensitive was O. surinamensis, whereas the most tolerant were S. granarius, R. dominica, and T. castaneum. Although an efficient fumigation within the spot was found, the efficacy was low in the grains surrounding the spot. The practical implications of the findings are discussed.