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With development of the market economy, the views concerning the importance of ethical norms in business activity are developing. Ethical orientation in economics, has been popularized for several decades by e.g. French centre "Economie et Humanisme"¹, established by a renowned Dominican, L.J. Lebret. Tourism, including rural tourism, represents an economic activity which has its specific ethical dimension. The goals of this paper are to characterize the basic values that are voluntarily accepted and used in tourism in rural areas in Poland and to identify the ethical norms that guarantee the realization of these values. The authors adopted the basic values-goals that should be recommended in rural tourism. They also formulated specific professional and ethical norms that support realization of the values, which include: humanity and standards for realizing the principle of humanity; the cultural and material conditions of living in rural areas; economic activity, including tourist activity that improves rural living conditions; innovative attitudes towards modernization and improving the quality of rural tourist services; and values that support the development of sustainable tourism in rural areas. The theoretical investigations concerning axiological problems were supplemented with the results obtained from a national-level empirical survey that was conducted in rural areas in Poland.

Chlorine, one of the most abundant elements in nature, undergoes a complex biogeochemical cycle in the environment, involved in the formation of volatile organochlorines (VOCls), which in turn can contribute to environmental problems, contaminate natural ecosystems, and are of public health concern. Several industrial and natural sources of VOCls have already been identified; however, data - particularly on the natural sources - are still scarce. The aim of this study was to investigate the diversity of emission of VOCls from soil and several undergrowth plants collected in temperate spruce forest ecosystem and the effect of salting on the VOCl emission of plants. Undergrowth plants were found to emit chloroform (CHCl₃) in the range of 2.2-201 pmol/day/g dry weight (DW), tetrachloromethane (CCl₄) 0-23.5 pmol/day/g DW, and tetrachloroethene (C₂Cl₄) 0-13.5 pmol/day/g DW; the average emission rates were about 10 times higher than that of soil (2.9-47.2; 0-5.8; 0-3.6 pmol/day/g DW of CHCl₃; CCl₄; C₂Cl₄ emission, respectively). Addition of sodium chloride solution in most cases caused an increase in the emission of CHCl₃ and caused a species specific - effect on the emissions of CCl₄ and C₂Cl₄. The results suggest that the emission of VOCls from spruce forest contribute to the atmospheric input of reactive chlorine; however, on a global scale it is only a minor net source.

The study presents the impact of management practices on greenhouse gas emissions (GHG) and nitrogen (N) losses calculated with a denitrification-decomposition model. Two cropping systems were analysed. The first rotation (A) consisted of potato, winter wheat, spring barley and corn. The second (B) included potato, winter wheat, spring barley and clover with grasses mixture. In A1 and B1 scenarios, fluxes were estimated on the basis of mineral fertilizers input, whereas in A2 and B2 scenarios the assessment of emissions was made with regards to manure. The results indicated that the application of manure in A rotation led to the increase of nitrous oxide (N₂O) emission, N leaching, N surplus, crop yields, and the decrease of nitrogen use efficiency higher than in B rotation. Additional doses of manure in A2 scenario increased the potential of the accumulation of soil organic carbon (SOC) and global warming potential (GWP) by 157%. In B2 scenario, SOC augmented more than three-fold but GWP increased only by 10%. The N losses and GHG emissions could be minimised by controlling N application through the implementation of nutrient management plan in which N doses are defined based on the crop needs and soil quality.

The role of orexin-A in the regulation of prolactin secretion in lambs born in different photoperiods was determined. The experiment was conducted with 30 female lambs. Pituitary glands were collected from 40-day-old lambs in three different seasons (n = 10 per each season) and photoperiods (light (L) hours to darkness (D) hours): long (May - L 14 : D 10), intermediate (August - L 13 : D 11), and short (December - L 10 : D 14). At 40 days of age, the lambs were decapitated, and their pituitaries were dissected and cut in half along the longitudinal fissure so that each half contained both glandular and nervous parts. The in vitro incubation of the glands was performed for 3 h in Parker's medium at 37°C. In each season, the control pituitary glands (K1, K2, K3) were incubated in Parker's medium, while the experimental pituitary glands (Ox1, Ox2, Ox3) were incubated in medium containing 1000 ng/ml of exogenous orexin. The administration of orexin-A during the long photoperiod (May) caused a significant (P ≤ 0.01) increase in prolactin secretion during the first hour of incubation (Ox1: 47.56 ± 8.4 ng/ml vs K1: 36.08 ± 7.8 ng/ml). During the intermediate photoperiod (August), a significantly (P ≤ 0.01) higher concentration of prolactin was observed in the first hour of incubation in the Ox2 group than in the control group (K2). During the first hour of incubation in December, the prolactin concentrations were significantly (P ≤ 0.01) higher in the Ox3 group than in the K3 group. Orexin-A increased prolactin secretion from pituitary explants collected from lambs born under all investigated time-points. The study results indicate that orexin-A is a stimulatory factor of prolactin secretion in sheep.

Forests occupy one third of the world's land area and govern carbon (C) transfers and influence nitrogen (N) content in the biosphere. Afforestation leads to soil changes of specific dynamics, often accompanied by acidification. Especially at higher altitudes this effect is accelerated and increased with the stand age since forestation. The change in soil C and N content following afforestation is controlled by a number of factors, including: previous land use (grasslands, cropland, etc.), tree species, soil cultivation method, soil properties (clay content, pH), stand age, site management, topography, and climate. In the Czech Republic, large area changes in land use took place in the last centuries - forests covering roughly 20% in the 18^th century currently occupy almost 34%, with still increasing tendencies. This paper compares basic soil properties (soil reaction, total soil organic carbon as well as total nitrogen contents) of the agricultural land and land afforested 40-60 years ago. The results confirmed the key role of afforestation in the change of soil organic matter dynamics after establishing new forests on the former agricultural lands in the uppermost mineral soil part of the Orlické hory Mts. region in the Czech Republic. During that time, comparatively substantial changes in soil organic matter and nitrogen were registered. Afforestation considerably increased organic matter content in the studied A-horizons of different land use types. Soil development resulted in a high production of C and N pools under the forest stands, contrary to agricultural land. In general, afforestation caused significant soil acidification. The common tendency of higher acidity of forest soils compared to agricultural ones was documented in the studied case as well. The general tendencies of soil reaction and soil organic matter dynamics at the studied sites are comparable to those in other regions of the Czech Republic.

Electroretinography (ERG) in the form of full-field, flash ERG is the most commonly used technique in veterinary ophthalmology for diagnosing the functioning of the outer retina. Under light stimulation spatially distributed different cell types within the retina produce time-varying electric responses. These are recorded in the form of ERG traces consisting of a series of positive and negative wavelets. The possibility of selective stimulation of individual types of retinal cells and the analysis of constituent components of ERGs are the basis for determining the source of abnormalities and diagnosis of various types of dysfunction. In many cases, the ERG allows diagnosis of hereditary retinal disorders in dogs before the appearance of behavioural and ophthalmoscopic symptoms. This review is an introduction to the electrophysiology of vision, intended for small animal practitioners, and aimed at presenting the benefits of ERG for early ophthalmic diagnostics in dogs.

Forty six Holstein Friesian repeat breeding cows (the average AI/conception was 5.2 ± 0.2) were investigated using the progesterone assay after AI to determine possible differences in plasma progesterone profiles between summer and winter seasons. Twenty eight (60.9%) and 18 (39.1%) cows were followed in summer (June-August) and winter (December-February), respectively. In the summer season, the total progesterone concentrations were higher (P < 0.05) in pregnant cows with normal luteal function compared to those in non-pregnant animals with abnormal luteal function. In contrast, in the winter season, there was no difference (P = 0.12) in total progesterone concentrations between pregnant and non-pregnant cows with normal or abnormal luteal functions. When the progesterone concentrations were compared, the pregnant and non-pregnant cows with normal luteal functions exhibited no difference (P = 0.92) in summer and winter seasons. Thus, the present study indicates that there is no effect of season on plasma progesterone profiles in repeat breeding cows; however in the summer season, the total progesterone concentrations were considerably higher in pregnant cows with normal luteal function compared to non-pregnant cows with abnormal luteal function.

Acute colic in horses, especially presentations requiring surgical correction, such as large colon volvulus, very often involves defective or excess platelet activation. The PFA-100 is a new point-of-care analyser that evaluates platelet function by measuring closure time (CT) in the whole blood of healthy horses in a standardised manner. However, there are no reports on platelet function in horses with colic measured by the PFA-100. The aim of the present study was to investigate platelet function in a group of horses suffering ascending colon displacement (ACD) and who underwent surgery compared to a group of control (C) horses. Thirty ACD cases and twenty untreated clinically healthy control horses were included. Blood samples were collected from the ACD horses prior to and 24, 48 and 72 h after laparotomy. Red blood cell count (RBC), haematocrit (HCT), haemoglobin (Hb) concentration, white blood cell count (WBC), total plasma protein (TP) concentration, platelet count (PLT), platelet volume (MPV) and ADP closure time (CT-ADP) were measured. CT was determined on a PFA-100® device using collagen/ADP cartridges. There were no significant differences in RBC, PLT and MPV between C and ACD horses pre-operatively. Reduced PLT was observed in the post-operative period. The mean CT-ADP of C horses was 96.43 ± 12.53 and 91.43 ± 2.51 seconds in the ACD group prior to surgery. CT-ADP was changed after surgery whereby CT-ADP time was more than doubled. We conclude that the PFA-100^® can be used to detect platelet function defects in horses.

We calculated high-power forwarder productivity in regeneration felling with the objective to set long-term work plans. Based on the time studies of the forwarder operator's work operation the operation time consumption was calculated and operation standards for the studied type of forwarder were designed. The standard values were determined based on the volume weight of harvested timber and the forwarding distance. The resulting time consumption required for forwarding 1 m³ of timber, whose mean tree volume is 0.30-0.99 m³.stem^-1, and upon forwarding distance of 100-1,000 m ranges from 0.05-0.09 Nh.m^-3.

For more than a century, and almost everywhere in the world, rural areas suffered a steady decline, due to the decrease of the real income from the agricultural activities, the lack of serious economic alternatives, and demographic issues. Rural tourism could contribute to the rural sustainable development through small local businesses valorising the natural, cultural and ethnographic resources. However, most of them need the financial support from private creditors (banks). The survey-based research conducted among banks' representatives has identified several strengths of small rural ventures, such as the small scale and the associated risks, the endurance to external shocks, the openness to market opportunities, as well as some of their weaknesses: e.g. a poor management experience, seasonality etc. We have also identified the actions necessary to be performed by these firms to attract financial resources from the banks' side, i.e. the diversification of income sources, the association and the adherence to recognized brands and maintaining a reasonable leverage ratio. It was found that the bank's size does not matter, but the nature of the bank's capital does: the private domestic banks are more willing to finance such businesses. The banks' involvement in co-financing the EU projects could be a way to foster and strengthen the rural businesses.

Planting grasses that require low maintenance is a good option for reducing the management input required. The objective of this study was to obtain information about turfgrass characteristics based on spaced-plant characterization and turf performance in turf plots of two selections (Paula hard fescue and Casero colonial bentgrass) recently released for public distribution by the Plant Production Area of the University of Oviedo. The plants were grown under conditions of no irrigation, no fertilizer, no pesticide application and minimal mowing over a 3-year period (2012-2014). The following morphological measurements were done in a spaced-plant nursery: heading date, plant height, inflorescence length and flag leaf length and width. The turf plots were evaluated for several traits including overall turfgrass quality (turf score), leaf texture and genetic colour. In both the spaced-plant nursery and turf trials, hard fescue genotypes displayed better morphological characteristics (narrow leaves, reduced plant height) and performed better (overall high turfgrass quality, fine leaf texture, dark green colour) than bentgrass genotypes under conditions of low maintenance. For areas in which climate, soil characteristics and management conditions are similar to those encountered in this trial, the selection of Paula hard fescue is recommended for use in low-maintenance turf settings because of the good turfgrass performance and morphological characteristics of this selection.

The aim of the studies was to determine the effect of the used herbicides and their mixtures on the level of glycoalkaloids (TGA) in the leaves and tubers of three edible potato cultivars. The studied factors were: I - three cultivars: Cekin, Satina and Tajfun. II - five ways of care: 1. mechanical care - the control object and four objects using herbicides Command 480 EC, Afalon Dispersion 450 SC and Stomp 400 SC. The content of glycoalkaloids both in the leaves and in the tubers of the edible potato significantly depended on the genotype. The highest content of TGA characterised the cv. Cekin - 623.5 mg/kg and 67.21 mg/kg of fresh weight (FW), respectively, while the smallest was found in cv. Satina - 339.3 mg/kg and 33.60 mg/kg of FW, respectively. Herbicides used in the experiment caused the increase of the content of glycoalkaloids compared to the tubers from the control object. However, a significantly higher concentration of TGA was stated after using the mixture of herbicides Command 480 EC + Afalon Dispersion 450 SC and Stomp 400 SC + Afalon Dispersion 450 SC.

The aim of the study was to evaluate egg shell quality characteristics, mineral content in the egg shell, and serum mineral concentration during the egg formation process as influenced by oviposition time and ambient temperature at the beginning and end of the laying cycle in laying hens and broiler breeders. Egg shell quality deteriorated significantly at the higher temperature and was lower in broiler breeders than in laying hens. The Ca (P = 0.047) and P (P = 0.018) contents of the egg shell were significantly higher at the higher temperature. The highest (P = 0.028) shell Ca content (352 g/kg) was in eggs laid in the morning, but the highest P (P = 0.030; 1.43 g/kg) and Mg (P = 0.001; 3.88 g/kg) contents were in eggs laid in the afternoon. Broiler breeder egg shells contained significantly more P (P = 0.004) and Mg (P = 0.001) than did those from laying hens. Serum Ca and P levels remained constant throughout the day whereas serum Mg and Zn levels decreased, the rate of decrease in Zn content being the same in all treatments, but with the amounts being greater in laying hens than broiler breeders. The results demonstrate that shell quality characteristics are more severely affected by different factors implemented in this trial compared to the shell mineral composition and especially compared to the serum mineral content.

The aim of this investigation was the analysis of the variability within the internal and external ophthalmic artery in the chinchilla (Chinchilla laniger, Molina). The head vasculature of 65 individuals was analysed, with particular emphasis on the internal ophthalmic artery originating from the central and rostral part of the cerebral arterial circle. Head blood vessels were filled with acrylic latex for vascular corrosion casting. The results showed ten variants of blood supply for the orbit, with a predominance of the first variant (66.1%) = bilateral presence of the external ophthalmic artery originating from the maxillary artery. Other variants differed in symmetry and asymmetry, sites of origination and the coexistence of both internal and external arteries. Vascularisation of the brain in chinchillas originates mainly from the vertebra-basilar system. The observed variability seems to confirm the role of the basilar artery in the arterial blood supply of the brain in this species.

The objective of this study was to determine the effect of canine inflammatory bowel disease (IBD) on the density of cocaine- and amphetamine-regulated transcript (CART) immunoreactive nerve fibres in the mucosa of different sections of the gastrointestinal tract. Fibre density was analysed in mucosal specimens from the duodenum, jejunum and descending colon of healthy dogs and patients with inflammatory bowel disease of varying intensity. Nervous fibres were stained in single-cell immunofluorescence assays. The density of CART-immunoreactive fibres was determined using a semi-quantitative approach by counting the number of fibres in the field of view (0.1 mm²). An increase in the density of CART-immunoreactive fibres was observed in dogs with moderate and severe IBD in comparison with healthy subjects. The results suggest that the presence of CART in enteric nerve fibres could play a role in the pathogenesis and development of canine IBD.

We aimed to evaluate the extractability of different extractants for zinc (Zn) and copper (Cu) in soils of long-term field experiments covering different agro-ecological zones of India. The relationships between the amounts of Zn and Cu extracted by Mehlich 3, 0.1 mol/L HCl and AB-DTPA (ammonium bicarbonate-diethylenetriaminepentaacetic acid) with those extracted by DTPA, the conventional extraction method widely used in soil testing laboratories in India, were elucidated. The treatments of the long-term experiments included control (no fertilizer), 100% NPK, 50%NPK + 50% N through FYM (farm yard manure) and a fallow soil. Some important physico-chemical properties of soil like pH, organic carbon, textural class, CaCO₃ content, etc. were analyzed. The NPK + FYM treatment was found to be the most effective treatment in terms of increased content of Zn and Cu in soils. The results showed that the amount of Zn and Cu extracted by Mehlich 3 were significantly correlated with that extracted by 0.1 mol/L HCl (r = 0.970** for Zn and r = 0.914** for Cu). Accordingly, Mehlich 3 and 0.1 mol/L HCl could be used effectively for estimating Zn and Cu availability in soils of India. However, Mehlich 3 was superior to all the other extractants used for the study.

The study was based on soil samples taken after the long-term fertilizer experiment (1974-2014) where different doses of nitrogen and potassium were applied. The experiment was located at the Research Station of the UTP University of Science and Technology in Bydgoszcz (Poland). The long-term application of nitrogen and potassium fertilizers leads to changes in the concentration of mercury, soil acidification, reduction in total organic carbon, total nitrogen as well as affluence of available nutrients (P, K, Mg) and increased mobility of copper and zinc. The significant positive correlation between total mercury content in the soil and the content of N-NO₃, Zn, N-NH₄ and the hydrolytic acidity value were stated.

The interactions between probiotic bacteria and the immune system in animals are known, but particularly in rabbits have not been sufficiently documented. Therefore the present study deals with the effect of the enterocin-producing probiotic strain Enterococcus faecium CCM7420 on the growth performance and jejunal morphometry in rabbits. Forty-eight weaned rabbits of male sex (NZB breed) at 5 weeks of age were randomly divided into experimental (EF) and control group (CG) with 24 rabbits in each group. Each day of the treatment period (21 days), the rabbits in the EF group were administered a fresh culture of the E. faecium CCM7420 strain (5.0 × 10⁸ CFU/animal/day in their drinking water); for the next 21 days after probiotic cessation the rabbits had untreated drinking water (the whole experiment lasted for 42 days). All animals had access to water ad libitum. Three animals from each group were randomly selected for slaughter at days 21 and 42 of the experiment to measure morphological parameters (villus height (VH), crypt depth (CD), VH : CD ratio) of the intestinal tissue in the proximal jejunum. The average daily gain was higher in EF group (39.49 g) than in CG (39.04 g). The jejunum VH, surface area, and VH : CD ratio increased, while CD decreased in EF group compared to CG. Inferring from these results, we conclude that administration of E. faecium CCM7420 strain to rabbits may improve weight gain due to better utilization of feed and larger absorption surface in the gut and also may positively influence the health status via enhancing the gut health in rabbits.

Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first tested with positive and negative control DNA and its sensitivity and specificity were determined. A new polymerase chain reaction protocol was developed by combining the new primer pair sets with bovine (12SM-FW and 12SBT-REV2) and ruminant host-specific primer sets (12S-FW and 12S-REV). In conclusion, the developed polymerase chain reaction assays can potentially be used for direct detection of Chlamydophila abortus in bovine and ruminant samples.

We studied effects of repeated applications of nitrogen on stem growth of mature Scots pine at four sites with yield potential around 3 - 4.5 m³ ha^‑1 yr^‑1. Treatments included control (0) and fertilization with ammonium nitrate at 100 and 200 kg N ha^‑1. Some plots were supplied with corresponding doses of urea. Applications were repeated up to five times, at five to twenty years intervals. Plot size was 22 x 22 m with buffer zones of 4 m. Measurements were made on an inner plot, 14 x 14 m. Tree ring widths usually reached a maximum two to four years after fertilization, returning to about the original level after seven to ten years. Responses were larger to 200 than to 100 kg N ha^‑1. Annual volume increment over the experimental period was 1.5 to 2.0 m³ ha^-1 larger in plots provided with 200 kg N ha^-1 than in control plots, or even more as the buffer zones of 4 m did not completely exclude tree roots from growing into neighbouring plots.

The effects of different concentrations of thyme (group T), clove (group Cl), and rosemary (group R) extracts on the microbiological, chemical, and sensory attributes of fish patty made from the bonito fish (Sarda sarda Bloch, 1793) were investigated. The numbers of total mesophilic aerobic bacteria, coliform bacteria, Staphylococcus-Micrococcus spp. and yeasts and moulds in group R were lower than those of the other groups during storage (P < 0.05). During the storage period, total volatile basic nitrogen (TVB-N), thiobarbituric acid index (TBA-i), peroxide values, and free fatty acid content gradually increased in all groups, and significant differences were found between the groups (P < 0.05). The results of sensory evaluation showed that the shelf life of fish patties was 4 days for the control group, and 8, 10, and 14 days for the groups treated with thyme, clove and rosemary essential oil, respectively (P < 0.05). In conclusion, the addition of essential oils showed a positive effect on the product shelf-life; and in particular, rosemary essential oil produced a remarkable effect.

Phosphorus (P) availability limits crop growth in most of cultivable soils in north-west India. The beneficial rhizosphere microorganisms such as phosphate-solubilising fungi (PSF) were found to increase P availability in soil and improve crop yields. In view of this, field experiments were conducted during 2009-2011 to evaluate the effect of seed inoculation with PSF (Penicillium bilaii) at different rates of fertilizer P on P content in leaves and grain yield of irrigated wheat in India. The soil was low in Olsen P at the Bathinda site and medium at the Ludhiana site. In no-P treatment, PSF significantly increased grain yield by 12.6% over non-inoculated control. The effect of PSF on grain yield was generally more pronounced in a soil with low Olsen-P compared to medium Olsen-P level. Inoculation of PSF along with 50% P fertilizer increased wheat yield equivalent to 100% P with no PSF. Spike density was significantly higher in PSF + 50% P than all the other treatments. There is need to study a long-term effect of Penicillium bilaii on P-fertilizer saving in wheat on soils varying in P availability, pH and P fixation capacity for different wheat-based cropping systems.

Based on a long-term fertilization experiment on a light soil, a study was conducted on the impact of varied fertilization on the levels of silicon forms available in the soil. It was shown that the exchangeable silicon content in the tested soil was very low, which can have a limiting effect on crop yields. Soil pH is a factor that significantly affects the exchangeable silicon content of the soil. Therefore, under the conditions of acidic soils, liming is a treatment that increases the level of silicon forms available to plants in the soil.

The impact of low temperatures combined with higher velocity of ventilated air in winter during severe frost in a free stall barn on the development of cold stress in Holstein-Friesian breed dairy cattle was studied. The study included measurements of outside and inside air parameters and cows' milk yield. Cold stress was evaluated in three technological groups (TG) using the WCT (Wind Chill Temperature) index. During the research, significant temperature and wind velocity differences (by up to 5°C and 0.5 m/s) were recorded in three zones of the barn occupied by the individual TG. All this resulted in different values of WCT. During most severe frosts lasting for 9 days, the average air temperature in the barn was -8.9°C (the lowest value was -17.3°C). The calculated average operative temperature for cows from all TG was as follows: -7.0°C for TG1, -11.1°C for TG2, and -12.3°C for TG3. It was also observed that animals from TG2 suffered from mild cold stress, which resulted in milk yield reduction by approximately 2 kg. It was concluded that there is a strong correlation (r = 0.72-0.89 with P < 0.05) between milk production and the WCT index. The measurements of most important microclimate parameters in the barn were conducted during winter seasons over the period of 2 years. They led to the conclusion that cattle kept in free stall barns are not vulnerable to the combination of low temperature and increased air movements. Research results of the present as well as of other authors were the basis for developing a table determining operative temperature for cows depending on the temperature of the environment and air movement velocity. Yet, the calculations and analysis of results show that there is a need to improve the applied calculation formula for operative temperature during cold weather.

Worldwide increasing energy demand is today permanently covered by a majority of non-renewable energy sources, namely by coal, crude oil and natural gas. This causes the rapid decline of their reserves and the time gets near when they will be run out. Therefore in the last years the exploitation of renewable energy sources has been permanently preferred. One of alternative fuel forms is fuel on the basis of paper waste. In this paper the results of tests are published, which were carried out using six sorts of recovered paper and board (group and grade 1.05, 1.06, 2.02, 2.05, 2.07 and 2.08 according to CSN EN 643:2002), pressed into the form of briquettes. During the tests following briquettes parameters were watched: moisture content, ash amount, gross calorific value, length and diameter, weight, density, rupture force and mechanical durability. It was proved that briquettes made from recovered paper and board compared with briquettes from wood waste are of high density, high mechanical durability and for their rupture, relatively high force is necessary. But at the same time they have high ash amount and low gross calorific value.

Water distribution in landscape is essentially influenced by the nature of the catchment rainfall-runoff process. Firstly, this paper presents an analysis of trends in the rainfall-runoff regime of the Lomnice river basin (South Bohemia, Czech Republic), using a single and double mass curve method. Secondly, the analysis of the rainfall-runoff regime is supplemented by an analysis of the evolution of temperature and snow conditions. Finally, a water balance analysis is performed for three selected years, using the Bilan hydrological model. The aim of the present paper was to find out (1) whether any changes have occurred in the trend of the rainfall-runoff regime of the catchment since 1970, and (2) to what extent these changes may have been caused by anthropogenic activities in the catchment. The results show several changes in the runoff scheme that are not related to the amount of precipitation. This statement follows from the comparison of single mass curves of precipitation and discharges. A comparison of the hydrological balance data for the Lomnice river basin, and the Otava and the Upper Vltava (a higher order stream) river basins led to the following conclusions: (1) There has been a change in the trend of the Lomnice river runoff regime. (2) The runoff regime of the Lomnice river basin differs from the regime of the higher order river basin (the Otava, the Upper Vltava). (3) The changes in the runoff regime in the Lomnice river basin were probably caused mainly by anthropogenic activities, the influence of which disappears within the larger basin (a higher order catchment area). Therefore, when planning sustainable water resources management, it is necessary to consider the impact of human activities not only globally, but also at the local scale.

Climate change, air pollution and global challenges. Understanding and perspectives from forest research R. Matyssek, N. Clarke, P. Cudlin, T.N. Mikkelsen, J.P. Tuovinen, G. Wieser, E. Paoletti Developments in Environmental Science 13. Elsevier Ltd., Oxford, 2013 622 pages, ISBN 978-0-08-098349-3. € 142.31.

The article is focused on food problem in the least developed countries, on the chosen areas where the overall situation is the most problematic. It deals with the Democratic Republic of the Congo, belonging to the low income food deficit countries with one of the world's lowest rates of the gross domestic product per capita. The paper defines the food security situation in a global connection, representing a wide complex of economic, social, demographic, technologic and political aspects of production, distribution, shift and consumption of foodstuffs. Despite some progresses in the political situation, the effects of the economic crisis and the widespread food insecurity are expected to persist. Te humanitarian assistance is now needed to support the needs of the most vulnerable. In the longer term, the countries have the significant goal of consolidating peace and security and strengthening the overall governance, while at the same time reconstructing and rehabilitating their economy.

Doubled haploidy breeding protocols have revolutionized the varietal development programmes in bread wheat, however, the protocols have not proved much efficient in durum wheat. Presently, the durum wheat × maize system is being widely followed for haploid induction but the frequency of haploid development is very poor which has limited its application in durum wheat improvement programmes. In order to formulate an efficient wide hybridization-mediated approach for haploid induction in durum wheat, different genotypes of durum wheat were subjected to hybridization with Imperata cylindrica, a wild perennial grass for the first time in this laboratory. The investigation was carried out for two seasons. During the 1^st year, the investigation was carried out on one genotype only, Langdon, in order to notice the development of haploid embryos and the factors that influence the haploid induction frequency. The most important factor influencing the embryo formation frequency was found to be the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Upon pollinating the emasculated spikes of durum wheat genotypes, various concentrations of 2,4-D were injected into the uppermost internode of wheat culm for three consecutive days after pollination to find out the most responding concentration for haploid induction. During the next year, the protocol was applied to eight durum wheat genotypes. The frequency of haploid induction parameters varied with the durum wheat genotypes as well as 2,4-D concentration used. The mean pseudoseed and embryo formation frequency over all the genotypes ranged from 30.2 to 56.3% and 1.2 to 18.4%, respectively. The average embryo formation frequency over all the genotypes was found to be highest (18.4%) at 250 mg/l 2,4-D whereas it was superior for the genotypes WH 896 and Langdon over all the 2,4-D concentrations. At the most responding 2,4-D concentration (250 mg/l), the genotype A-9-30-1 exhibited the highest embryo formation frequency (32.1%). The ploidy status of the developed embryos was identified using cytological analysis carried out on the rootlets of the tissue culture generated plantlets.

The study evaluates the effect of liming materials application in combination with NPK fertilizer and borax on macronutrient contents (nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg)), in an extremely acid soil and raspberry leaves and fruits during a two-year period. Liming increased soil pH, N mineral content, P, Ca and Mg soil content, while K content either increased (dolomite and borax application), or decreased (lime application). The N and P contents in raspberry leaves after liming increased significantly, but P content remained below the optimal values. Some treatments with lime caused a decrease in K content in leaves, while dolomite and borax application increased K content. Initially optimal Ca content in leaves increased significantly in the treatments with lime, but decreased after dolomite application. The Mg content in leaves increased after dolomite and borax application, but mainly remained below optimal values. Liming either did not alter or only slightly altered macronutrient contents in raspberry fruits.