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Synergic strategies in reinforcing the pluralistic paradigm, inclusion and diversity as a catalyst for social sustainability focusing on agribusinessesOriginal PaperHana Urbancová, Pavla Vrabcová, Peter Madzík, Ale¹ KocourekAgric. Econ. - Czech, 2025, 71(5):273-284 | DOI: 10.17221/371/2024-AGRICECON Addressing social sustainability, gender dynamics, strengthening educational diversity and ensuring inclusive partnership structures are all key components of promoting inclusion, not only in the agricultural sector. Diversity management is a human resource trend based on respect for inclusive culture, gender and ethnic diversity, multifaceted equality or the use of virtual teams under the right working conditions, but when not applied correctly it may produce undesired effects in agribusinesses as well as in other economics sectors. This paper aims to identify effective approaches to setting working conditions used by organisations to promote inclusion, management diversity and the development of social sustainability. The data were obtained through quantitative research using the Computer Assisted Web Interviewing method (n = 202) and processed using the chi-square test at a 0.05 significance level as well as through qualitative research involving focus groups (n = 10). The study results have demonstrated that investment in diversity education remains crucial. Not only agricultural but also other businesses have begun to use metrics to monitor their diversity and inclusion progress. Organisations are beginning to use artificial intelligence (AI) to remove prejudices in recruitment, identify inequalities in remuneration and ensure inclusion in online communication. |
Classification of peanut variety based on hyperspectral imaging and improved extreme learning machineOriginal PaperMengke Wang, Hongrui Zhang, Hongfei Lv, Chengye Liu, Jinhuan Xu, Xiangdong LiCzech J. Food Sci., 2025, 43(1):17-28 | DOI: 10.17221/109/2024-CJFS Peanut as an important crop, plays an important role in agricultural production, which is rich in edible vegetable oil and protein. The variety of peanut affects the content of vegetable oil and protein. Therefore, the classification of peanut variety can better promote the sustainable development of agriculture. In this study, hyperspectral imaging technology is used to achieve peanut variety classification. In addition, the spatial-spectral extreme learning machine (SS-ELM) is proposed to process the hyperspectral data to get the final classification label. To fully explore the spatial structure information of hyperspectral data, propagation filtering is integrated into the framework of extreme learning machine (ELM). The average accuracy of the improved ELM model on five varieties of peanuts dataset (Luhua 11, Dabaisha, Xiaobaisha, Fenghua, and Luohanguo 308) is 98.32%, which is higher than other classic models. The experimental results show that the improved ELM can classify peanut of different varieties by hyperspectral imaging. |
Analysis of ejaculate parameters and sperm morphology in roosters of initial laying strainsOriginal PaperLadislav Máchal, Tomá¹ Kopec, Zuzana Reèková, Radek Filipèík, Milan Veèeøa, Vojtìch Pe¹an, Eva TùmováCzech J. Anim. Sci., 2025, 70(2):72-81 | DOI: 10.17221/203/2024-CJAS
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Whole-genome resequencing data reveal the genetic diversity of local chickens in southern Zhejiang and surrounding areas in ChinaOriginal PaperYawen Zhang, Bo Zhang, Ziwei Li, Fengxiang Hou, Yan Zhao, Junjie Jin, Bin Song, Wenjie GuCzech J. Anim. Sci., 2025, 70(3):113-120 | DOI: 10.17221/186/2024-CJAS China’s local chicken breeds, especially those in the Zhejiang Province, are rich in genetic diversity owing to environmental heterogeneity and complex ecosystems. Distinctive local breeds have emerged through long-term natural selection and domestication. We investigated the genetic diversity and population structure of local chickens in southern Zhejiang and surrounding areas using whole-genome resequencing of 129 chickens from seven populations. A total of 1.8 terabytes of raw data was obtained, and 4 802 728 single nucleotide polymorphisms were detected. The Xianju chicken population exhibited the highest genetic diversity, while Yandang Partridge chickens were genetically distant from other chicken breeds. This study provides valuable information for conserving poultry genetic diversity and informs about breeding programmes of local Chinese chicken breeds. |
Reciprocal hybridisation of Aloe species (Aloe arborescens with A. vera) and their characterisation in a highland region of VenezuelaOriginal PaperJosé Imery-Buiza, Wendy Ozols-NarbonaCzech J. Genet. Plant Breed., 2025, 61(2):100-109 | DOI: 10.17221/7/2025-CJGPB Aloe vera and A. arborescens are succulent plants widely used in cosmetics, pharmaceuticals, and food supplements. The objective of this study was to perform interspecific crosses and agronomically characterise three genotypes cultivated in a Venezuelan highland region (altitude 1 727 m, 13–17.9 °C). Successful hybridisation was achieved only when A. arborescens pollen (P1) was used on A. vera pistils (P2), whereas the reciprocal cross was largely unsuccessful. Hybrid seed germination reached 11.86%, and adult hybrids exhibited significant vegetative superiority over P1, particularly in leaf volume, leaf weight, and flower number. The progeny outperformed both parents in leaf base width and lateral tooth size, thereby enhancing its ornamental value. The expected 2n = 2x = 14 karyotype was confirmed in the root meristems of hybrids. The analysis of leaf pulp processing indicated that the hybrid was promising for juice production (39.8% yield, 1 203 ppm acemannan), thus highlighting its potential for agro-industrial applications in tropical highlands or comparable temperate regions. Other agronomic traits, including number, thickness, colour, and shape of leaves; sprouting of stem branches and basal suckers, flowering period, inflorescence, eggs/ovary, and details of the colour and dimensions of bracts, perianth, pedicel, and ovary, were also evaluated. |
Perception of bimodal warning cues during remote supervision of autonomous agricultural machinesOriginal PaperAnita Chidera Ezeagba, Cheryl Mary Glazebrook, Daniel Delmar MannRes. Agr. Eng., 2025, 71(2):69-79 | DOI: 10.17221/73/2024-RAE Agricultural machines that are fully autonomous will still need human supervisors to monitor and troubleshoot system failures. Recognising the emergency as soon as possible is crucial to reduce adverse effects. The ability of humans to detect visual, auditory, or tactile cues is usually enabled by warning systems. The effectiveness of different warning cues varies in terms of prompting a quick response. The study’s objective was to compare the effectiveness of two bimodal warnings (i.e., visual-auditory and visual-tactile) at eliciting supervisor perception (which equates to level one situation awareness). Twenty-five participants engaged in an autonomous sprayer simulation. Two realistic remote supervision scenarios (i.e., in-field and close-to-field) were used to examine two bimodal warning cues: (i) visual-auditory and (ii) visual-tactile. The effectiveness of each bimodal warning was assessed based on two measures: (i) response time and (ii) noticeability. There was no significant difference between the bimodal warning cues in terms of response time when tractor sound was present in the experimental environment (reflecting the in-field remote supervision scenario); however, visual-tactile cues yielded shorter response times than visual-auditory cues when the experimental environment was quiet (reflecting the close-to-field remote supervision scenario). There were no statistically significant differences between visual-auditory and visual-tactile warnings concerning noticeability. Participants’ subjective answers indicated they preferred the visual-tactile cues better than the visual-auditory cues. It is concluded that visual-tactile warnings are preferred over visual-auditory warnings to enable perception during remote supervision of autonomous agricultural machines (AAMs). |
Cytotoxic and apoptotic effects of etoposide and ellagic acid alone or with possible synergistic/additive combinations on a canine D-17 osteosarcoma cell lineOriginal PaperGS Ekren Asici, F Kiral, A Bildik, PA UlutasVet Med - Czech, 2025, 70(8):279-293 | DOI: 10.17221/24/2025-VETMED Osteosarcoma (OSA) is the most prevalent and aggressive malignancy of canine bones. Etoposide is an effective chemotherapeutic agent for cancer treatment, whereas ellagic acid is a naturally occurring compound with antioxidant and anticancer properties; however, both are inhibitors of the topoisomerase enzyme. In this study, the synergistic/additive effect of etoposide, known to have a growth-inhibitory impact in canine osteosarcoma (OSA) cells, and ellagic acid was investigated. The mechanism by which this effect inhibits cell growth at lower etoposide doses was also examined. The IC₅₀ values of both agents were determined, and possible combination doses were generated accordingly and applied to canine OSA cells. The apoptotic effects of the combinations were evaluated based on DNA breaks and the activity levels of caspase 3, 8, and 9. These findings were supported by the expression levels of Bcl-2, Bax, and Bid genes, as well as the AO/EtBr staining method. The effects on cell cycle and proliferation were analysed through survivin and NF-κβ gene expressions. Antimetastatic effects were determined using invasion and migration assays. EA is a potential therapeutic agent for cancer treatment. In combination with ET, a higher anticancer efficacy was demonstrated compared to etoposide alone. Potential treatment side effects can be reduced by enabling the use of lower drug doses. |
The impact of the integration of science, technology, and finance on innovation in listed agricultural enterprises through the lens of digital transformationOriginal PaperFanye Kong, Huini Zhong, Nana Chai, Baofeng ShiAgric. Econ. - Czech, 2026, 72(2):84-100 | DOI: 10.17221/22/2025-AGRICECON Digitisation enables the integration of science, technology and finance, and this trend toward integration can in turn enhance the innovation efficiency of listed agricultural enterprises. By leveraging the quasi-natural experiment of China's 2011 pilot program for technology finance – also known as science, technology, and finance, this paper uses the difference-in-differences (DID) model to demonstrate that the science and technology finance policy positively impacts the innovation levels of agriculture-related listed enterprises. That said, the policy's effect varies across different regions and enterprise sizes. Furthermore, moderating-effect analysis reveals that the digitalisation level of agriculture-related listed enterprises is a critical factor, while the evolutionary game model elucidates this factor's underlying mechanism. Based on these findings, policy recommendations are proposed to further develop science and technology finance platforms for agricultural-related listed enterprises. |
Free electricity tandem-twin-hybrid solar-biomass dryer increased the performance of coffee cherry dryingOriginal PaperYuwana Yuwana, Syafnil SyafnilRes. Agr. Eng., 2025, 71(3):174-187 | DOI: 10.17221/98/2024-RAE
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Efficacy of a new ready-to-use PCV2 and Mycoplasma hyopneumoniae vaccine under field conditionsOriginal PaperP Trampus, A Csagola, T Szalai, N Palmai, A Toth, N Terenyi, Z Nagy, NA Szeplaki, G Somogyi, E Rausch, Z Penzes, R KrejciVet Med - Czech, 2025, 70(11):401-407 | DOI: 10.17221/25/2025-VETMED Coinfection of porcine circovirus type 2 (PCV2) with Mycoplasma hyopneumoniae (M. hyo) causes major worldwide economic losses within the swine industry. This study aimed to assess the safety and efficacy of a single dose of a bivalent vaccine containing PCV2d and M. hyo antigen (Cirbloc® M Hyo) under field conditions. Two studies were performed under the GCP (Good Clinical Practice) requirements on farrow-to-finish farms in Hungary and Cyprus. On both farms, the presence of both PCV2 and M. hyo infection was demonstrated. For both studies, safety parameters were observed and measured from inclusion at 21 (±3) days of age until 14 days after vaccination. Efficacy parameters were observed and measured from inclusion until slaughter. Administration of the vaccine was safe in both studies, as no general, immediate, or local reactions were observed. The efficacy of the vaccine was confirmed in both studies as the following parameters were significantly reduced in the vaccinated groups compared to the control groups: viraemia, faecal shedding, viral load in lungs and in all collected lymphoid tissues, M. hyo-specific lung lesions, and average daily body weight gain. These results collectively support the vaccine’s potential as an effective tool for disease control. |
Rainfall pattern impact on runoff and sediment of the sloping cropland in Northeast ChinaOriginal PaperXu Fan, Wei Hu, Zhongzheng Ren, Yuan Chen, Qingsong Shen, Xingyi ZhangSoil & Water Res., 2026, 21(1):9-19 | DOI: 10.17221/39/2025-SWR Rainfall is a major contributor to water erosion of sloping cropland in Northeast China. Identifying how rainfall and slope gradient (S) influence runoff depth (RD) and sediment yield (SY) is crucial for preventing water erosion. Field measurements from runoff plots were collected from 2023 to 2024, and K-means clustering was applied to clarify the rainfall patterns. Response of RD and SY to the rainfall pattern and S were analysed. Key factors impacting RD and SY were explored. The results showed that three rainfall patterns were identified for 34 erosive rainfall events: A (41.2%, medium duration, medium rainfall intensity, and medium rainfall amount (RA)). B (50.0%, short duration, high rainfall intensity, and low RA) and C (5.4%, long duration, low rainfall intensity, high RA). Furthermore, the cumulative RD and SY increased with S for the same rainfall pattern. The cumulative RD and SY responded similarly to rainfall patterns for the same S. The contribution of the rainfall pattern to the cumulative RD and SY decreased in the order of C, A, and B. In addition, rainfall duration (D) and maximum 30-minute rainfall intensity were the key factors affecting RD and SY for rainfall pattern A, respectively. Rainfall erosivity (R) was the key factor affecting RD and SY for rainfall pattern B and C. R and RD were the dominant factors influencing the RD and SY for all rainfall events, respectively. |
Foliar silicon modulates structural and biochemical responses of buckwheat to water deficitOriginal PaperJiri Krucky, Vaclav Hejnak, Pavla Vachova, Jana Ceska, Jan Kubes, Milan SkalickýPlant Soil Environ., 2026, 72(1):66-75 | DOI: 10.17221/539/2025-PSE Drought is a major abiotic stressor that limits crop growth and is often associated with oxidative stress. We evaluated whether foliar silicon (Si) application affects primary root anatomy, plant height, and phenolic metabolism in three common buckwheat (Fagopyrum esculentum) cultivars (La Harpe, Panda, and Smuga) exposed to water deficit. Plants were grown under controlled conditions in four treatments: control; drought; control + Si, and drought + Si. Qualitative anatomical assessment revealed that Si promoted more advanced development of the primary root central cylinder, most notably in La Harpe under drought conditions, where a continuous ring of secondary xylem and a well-developed pith were observed. Drought significantly reduced plant height in all cultivars; Si partially alleviated this reduction in La Harpe and Panda, but not in Smuga. Drought generally increased total phenolic content (TPC) and phenolic acid content (PAC) in both leaves and roots, and Si further enhanced these responses, with the highest values under drought + Si. Overall, the results indicate cultivar-dependent effectiveness of foliar silicon (Si) and suggest that Si contributes to coordinated structural and biochemical adjustments under water deficit conditions. To assess the transferability of these responses, further verification across a broader range of genotypes and under different intensities and durations of drought is warranted. |
Modelling the hydration process of wheat grain with layer-dependent diffusion coefficientsOriginal PaperBakhtiyar Ismailov, Abdushukur Urinboev, Khairulla Ismailov, Akmaljon KuchkarovRes. Agr. Eng., 2026, 72(1):25-40 | DOI: 10.17221/101/2025-RAE This study develops and validates a multilayer diffusion model of wheat grain hydration that incorporates layer-dependent diffusion coefficients for bran, endosperm, and germ. The moisture transport is formulated using Fick’s law with two interface formulations: (i) classical continuity of the concentration and flux and (ii) an interlayer resistance formulation that permits concentration discontinuities. Diffusion coefficients and geometric parameters were determined experimentally; A 3D grain model (structured-light scanning, COMSOL Multiphysics) informed the computational domain. Numerical solutions combined eigenfunction expansions with finite-difference discretisation near the interfaces. Across eight winter wheat varieties, the diffusion coefficients spanned 11.6 – 20.5 × 10–12m2·s–1 (mean 16.27 ± 3.08 × 10–12m2·s–1 ). Relative to the continuity model, the resistance model reduced the early-stage endosperm over-prediction by ~ 0.6–1.0 % (absolute) and lowered the whole-grain RMSE by ~ 20–30% over 0–240 min. These results support the role of thin moisture-retaining films as active barriers and yield smooth, real-time-ready outputs suitable for the automated control of pre-milling hydration; the framework is extensible to full 3D transient simulations. |
Evaluation of the effect of different thinning types on dendrometric parameters and subsequent spontaneous growth in a beech-oak-linden standOriginal PaperKateøina Novosadová, Jiøí Kadlec, Petr Sýkora, Martin Kománek, Radek PokornýJ. For. Sci., 2024, 70(6):299-316 | DOI: 10.17221/10/2024-JFS Due to an increasing risk of further damage to forests, forest managers are considering introducing an alternative direction for their future development – via the cultivation of mixed forests. At middle altitudes in the Czech Republic, an oak-beech-linden stand is the most natural type, and we tried to answer three main questions: (i) How the various thinning types affect dendrometric parameters and quality of the stand; (ii) How long thinning works on this stand until it loses its effect; (iii) How the stand develops spontaneously after abandonment. This experiment was conducted at the Training Forest Enterprise in the Czech Republic in Drahanská vrchovina (highlands in central Moravia). In 1988, four plots were established in a 49-year-old stand where, in three of the plots, different types of thinning (crown, low and heavy crown) were performed, leaving one (reference plot) to develop naturally. The height, the height of the crown base and diameter at breast height (DBH) were measured, and the shape and quality of the trunk and crown were estimated on each tree. Measurements were carried out in 1989, 1994, 1999, 2005, 2010, 2015, and 2020. In the first 10 years, the DBH and height of the crown base did not show any differences, and the linden at the heavy crown plot outgrew the linden trees at the other plots in height. After these 10 years, the thickest linden, the tallest beech and linden, and the greatest height of the crown base of beech and linden were all found at the heavy crown plot. The shape and quality of the trunks and crowns of beech, oak and linden were similar in all plots (including the reference plot) during the entire experiment. After thinning, the plots were left to grow spontaneously. The heavy crown thinning removed a greater number of thicker trees at the middle level, thus supporting the trees growing in the lower part of the middle level and in the below level (i.e. the beech and linden). These trees then grew more quickly compared to the others, but their quality decreased, as did that of the others. Therefore, a forest left to grow and develop spontaneously is practically unusable for commercial purposes. |
Impacts of management and changed hydrology on soil microbial communities in a floodplain forestOriginal PaperJiøí Volánek, Martin Valtera, Ladislav Holík, Martin Kománek, Hana Burdová, Josef Trögl, Diana Polanská Nebeská, Jitka Novotná, Pavel Samec, David JuøièkaJ. For. Sci., 2024, 70(11):574-592 | DOI: 10.17221/44/2024-JFS Long-term human activities substantially altered floodplain regions of temperate Europe. Forest management and extensive changes in hydrology greatly affected natural floodplain soil properties, in which microbes play key roles. This study aims to assess the effects of human activities through a gradient of forest management intensity on soil microbial community (SMC), its biomass, activity, and structure. Soil chemical and physical-chemical properties were used to explain the general associations and within-site variation using principal component analysis (PCA), linear regression (LR) and linear mixed-effect regression (LMER) models. It was found that forest management application, regardless of its intensity, led to significant microbial biomass reduction. PCA revealed that microbial biomass, expressed as a sum of phospholipid fatty acids along with recalcitrant carbon fraction (ROC) best explained the variability in data. LR and LMER highlighted that bacteria are affected by floodplain forest management more than fungi, and that bacterial response to pH was highly diversified. Also, pH was identified as the best predictor of SMC structure and activity but not of its size. The study calls for further investigation in SMC interactions with ROC, soil-available Fe and Mn, and the role of redox-active metals in soil organic carbon degradation. |
Dissecting the osmotic and oxidative stress responses in salt-tolerant and salt-sensitive wheat genotypes under saline conditionsOriginal PaperUlkar Ibrahimova, Javanshir Talai, Md. Mahadi Hasan, Irada Huseynova, Vaseem Raja, Anshu Rastogi, Hamideh Ghaffari, Marek Zivcak, Xinghong Yang, Marian BresticPlant Soil Environ., 2025, 71(1):36-47 | DOI: 10.17221/459/2024-PSE Salinity represents a significant abiotic stress that markedly influences plant growth through osmotic stress induction. Plants commonly undergo osmotic adaptation when subjected to prolonged periods of saline stress. The current experiments were conducted on five wheat (Triticum aestivum L.) genotypes with contrasting salt tolerance capacities – Mirbashir 128, Gobustan, Gyzyl bughda, Fatima, and Zirva 80 under salinity stress caused by 150 mmol NaCl. The relative water content and osmotic potential were found to decrease significantly in salinity-sensitive genotypes (Fatima and Zirva 80) compared to salinity-tolerant ones (Mirbashir 128, Gobustan, and Gyzyl bughda) when treated with 150 mmol NaCl. Salinity also caused the accumulation of soluble sugars and proline, the amounts of which were observed to be higher in salinity-tolerant genotypes than sensitive ones, while lipid peroxidation was higher in salinity-sensitive genotypes. In salinity-tolerant genotypes, 150 mmol NaCl caused increased antioxidant enzyme activities and accumulation of flavonoids, including anthocyanins, confirming the rapid development of the stress reactions in these plants. Differences in the osmoregulation indicators and antioxidant responses between salinity-tolerant and sensitive plants are assumed to be related to their salinity-tolerance traits. This investigation provides pivotal foundational insights for enhancing the salt tolerance of wheat genotypes, thereby potentially enhancing both yield and quality in diverse wheat cultivars thriving in saline environments. |
Fractal parameters of soil particle size distribution in karst area, and implications of soil water repellency by plantationsOriginal PaperZhuo Tian, Haitao Deng, Shuaipu Zhang, Qinxue Xu, Xin JinSoil & Water Res., 2025, 20(2):93-104 | DOI: 10.17221/84/2024-SWR As a critical and universal soil physical property, soil water repellency significantly affects soil and water erosion and vegetation restoration, particularly in the karst region. This study analysed soil properties, namely, particle size distribution (PSD) in abandoned farmland and different plantations in karst areas, and their impact on water repellency to provide references for its ecological restoration. First, for the shaddock plantation, citrus plantation, ginkgo plantation, Robinia pseudoacacia plantation, and abandoned farmland, the research objects, soil particle size and water drop penetration time were measured, and the fractal parameters of soil PSD were computed. Soil PSD characteristics’ influence on water repellency was inferred according to, for example, correlation, redundancy, and stepwise regression analyses. Sand content in the shaddock plantation and clay content in the citrus plantation was the highest. The soil particles of abandoned farmland and plantations were primarily sand. The values of volume dimension D(0) in descending order were citrus plantation, shaddock plantation, ginkgo plantation, R. pseudoacacia plantation, and abandoned farmland. The values of information dimension D(1), correlation dimension D(2), and information dimension/volume dimension D(1)/D(0) in descending order were R. pseudoacacia plantation, ginkgo plantation, citrus plantation, abandoned farmland, and shaddock plantation. No significant differences were found in the symmetry degree Df, the spectral width Da of the singular spectral function, and the single fractal dimension D among the abandoned farmland and different plantations. Slight water repellency was observed in the abandoned farmland and different plantations. The degree of water repellency in descending order was R. pseudoacacia plantation > ginkgo plantation > abandoned farmland > citrus plantation > shaddock plantation. Significant correlations were found between fractal parameters and water repellency, and fractal parameters were suitable potential indicators for soil water repellency. |
Shared knowledge and competitive advantages for economic diversification through olive oil tourismOriginal PaperEdianny Carballo-Cruz, José Domingo Sánchez-MartínezAgric. Econ. - Czech, 2026, 72(2):69-83 | DOI: 10.17221/344/2024-AGRICECON The economic diversification of rural territories specialised in olive growing is key to the sustainability and resilience of these communities. In this context, olive oil tourism is presented as an innovative strategy to add value to the olive sector, attract visitors and promote public–private synergies. This study aims to determine the competitive advantages of olive oil tourism in the province of Jaén, Spain, using strategic analysis tools that align tourism resources with effective management strategies. Hence, in addition to a questionnaire completed by experts and a specialised literature review, we used the Quality Function Deployment (QFD) matrix, adapted to territorial analysis focused on tourism. This methodology correlates the territorial resources of olive oil tourism (TRs, the ‚what') with management specifications (MSs, the ‚how'). The analysis determines the relative importance of the TRs, the objective values and the technical importance of the MSs, and the direction of improvement. Furthermore, it includes an evaluation of competitiveness and estimates the technical-competitive positioning index. Nine TRs and eight MSs were identified and correlated, and Jaén's performance was compared to that of six other Spanish olive-growing provinces. Nine competitive advantages were identified, linked to five TRs (olive grove heritage landscape, tasting areas, exhibition centres, olive oil mills and greenways) and four MSs (olive mills, environmental education, diversification of supply and heritage promotion and innovation). This work proposes a useful tool to align resources and management, improve decision-making and move towards a more strategic governance in the olive oil tourism sector. |
Effect of treatment of narcissus bulbs with hydrogen peroxide with silver and fungicides on plant growth and developmentOriginal PaperAdam Tadeusz Wojdy³a, Jacek Stanislaw Nowak, Jan Bocianowski, Artur Kowalski, Jacek Wi¶niewski, Emilia WaszkiewiczHort. Sci. (Prague), 2025, 52(1):81-90 | DOI: 10.17221/43/2024-HORTSCI The aim of the research was to demonstrate the possibility of using hydrogen peroxide with silver (H2O2-Ag+) and selected fungicides for treating narcissus bulbs and their impact on plant growth and development. In the experiments were used compounds such as hydrogen peroxide with silver (H2O2-Ag+) and captan (Biszop 80 WG), pyraclostrobin + boscalid (Signum 33 WG) and methyl thiophanate + tetraconazole (Yamato 303 SE) to soak narcissus bulbs before planting for the period of 20 minutes. The research showed that stimulation of growth and development by some of the tested concentrations of H2O2-Ag+ used to treat bulbs was shown with regard to plant height, leaf length, fresh flower weight, dry flower weight, the number of days from taking plants out of cold storage to flowering, the weight of fresh above-ground parts without flowers, the weight of dry above-ground parts without flowers, the diameter of the flowers, the height of the flowers, petals length, the width of the petals and the length of the corolla. In turn, the tested fungicides stimulated the length of leaves, the fresh weight of plants without flowers, and Signum 33 WG and Biszop 80 WG also the dry weight of flowers. No phytotoxicity of hydrogen peroxide with silver and tested fungicides was found for narcissus. |
The economic aspects of organic farms selling their products to organic or conventional marketCase StudyZuzana Fuksová, Iveta Bo¹ková, Jana Hlaváèková, Marek NovákAgric. Econ. - Czech, 2025, 71(4):218-227 | DOI: 10.17221/370/2024-AGRICECON Organic farming is a topic often discussed in the scientific community and public space. The current European policy strongly supports the enhancement of organic farming concerning the environment, sustainability, and social aspects. It is generally assumed that a lower intensity of production, yields, livestock utility, fertilisation, and total costs should be compensated by premium prices, subsidies, or higher economic performance. Research revealed that achieving premium prices for organic products on the market is not obvious for organic farmers as some organic commodities have been sold on the conventional market. Our study focused on the price difference reached by the sample of farms on the organic and conventional markets for two organic commodities (winter wheat and milk) and the farm's economic results. We combined and compared data from two unique databases on organic farming in the Czech Republic. We used the statistical methods of ANOVA and regression for analysis. We found the difference between evaluated market prices. Price variability is wide in both types of organic and conventional markets but the influence of the prices on farm performance was not significant. |
Environmental DNA (eDNA) contamination patterns of African swine fever virus (ASFV) in swine transport vehicles in the PhilippinesOriginal PaperMJ Bartolome, LAM Aguirre, CM Poliquit, I Besas, JG Angeles, J Rabajante, S Camba, F Del Valle, A Ambrocio, F Caguicla, MG Bustamante, D UmaliVet Med - Czech, 2025, 70(5):156-165 | DOI: 10.17221/84/2024-VETMED Since its introduction in 2019, African swine fever (ASF) has spread to all regions of the Philippines, affecting 73 out of its 82 provinces. To assess the environmental DNA (eDNA) contamination patterns of the ASF virus (ASFV) in swine transport vehicles and evaluate its measures of association, a total of 450 environmental swabs from 30 transportation vehicles were tested using qPCR. Five out of 30 vehicles (16.67%) tested positive in at least one of the following areas: cargo area or sidecar walls (6.67%), cargo area or sidecar floors (6.67%), hauling personnel’s hands (6.67%), steering wheel or handlebars (3.33%), gear shift levers (3.33%), floor mats or footpegs (3.33%), dashboards (3.33%), door handles or sidecar gate bolts (3.33%), tyres/wheels (3.33%), fenders (3.33%), and hauling personnel’s footwear (3.33%). All investigated risk factors were analysed using Chi-square, Fisher’s exact test, and odds ratio and were found to be insignificant, including the frequency of swine transportation per week, frequency of cleaning per week, cleaning materials used, disinfection practices, the number of pigs transported, and whether hauliers owned pigs (p > 0.05). This study illuminates the environmental contamination patterns of ASFV in swine transport vehicles, underscoring the need for targeted biosecurity protocols in areas with a high risk of contamination, particularly the cabin areas. More effective vehicle disinfection systems that will not damage equipment or pose health risks to hauliers are recommended to reduce the risk of ASF disease transmission between countries and geographic regions. |
Influence of the amount of water on the growth of one species and four hybrids of Paulownia in the first year of cultivation in Central EuropeOriginal PaperJiøí Kadlec, Kateøina Novosadová, Martin Kománek, Radek PokornýJ. For. Sci., 2025, 71(6):286-296 | DOI: 10.17221/19/2025-JFS
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Salt stress mitigation in chickpea seedlings: a comparative study of zinc oxide nano and bulk particlesOriginal PaperNadiyah M. AlabdallahPlant Soil Environ., 2025, 71(6):398-408 | DOI: 10.17221/115/2025-PSE Nanotechnology plays a vital role in enhancing plant tolerance to salt stress; however, comparative studies on zinc oxide bulk particles (ZnO bulk) and zinc oxide nanoparticles (ZnO NPs) in this context remain unexplored. Since zinc (Zn) is an essential micronutrient involved in enzyme activation, photosynthesis, and antioxidant responses, it is important to understand how ZnO bulk and ZnO NPs influence chickpea growth under salt stress. This study investigated the morphological and physiological responses of chickpea seedlings treated with ZnO bulk (50 mg/L) and ZnO NPs (50 mg/L) under varying salt concentrations (20, 40, 80, and 120 mmol/L). Salt stress significantly inhibited chickpea growth, reducing the relative growth rate, net assimilation rate, total chlorophyll content, and potassium (K) and zinc ion levels while increasing sodium (Na), chlorine (Cl), malondialdehyde (MDA), and proline content. However, the application of ZnO bulk and ZnO NPs improved these parameters, mitigating the negative effects of salt stress. Furthermore, exogenous ZnO bulk and ZnO NPs to salt-stressed (20, 40, 80, and 120 mmol/L) chickpea resulted in decreased malondialdehyde content by 30, 32, 47, 34%, and 58, 31, 48, 47%, proline content by 4, 6, 1.6, 4% and 22, 21, 22, 28%, respectively, in comparison to the control. Notably, ZnO bulk and ZnO NPs enhanced antioxidant enzyme activities, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione peroxidase, and glutathione reductase. These findings suggest that foliar application of ZnO bulk and ZnO NPs helps alleviate salt stress in chickpeas, promoting better growth and physiological performance under saline conditions. |
Effect of dehulled lupin seed meal in feed mixture on muscle fat quality of Cherry Valley ducksOriginal PaperEva Straková, Lucie V¹etièková, Pavel Suchý, Martin Kutlva¹rCzech J. Anim. Sci., 2025, 70(7):276-285 | DOI: 10.17221/76/2025-CJAS
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Consumer sensory evaluation of flavour enhancers derived from snail protein hydrolysate using the Rate-All-That-Applies methodOriginal PaperDedin Finatsiyatull Rosida, Dina Mustika Rini, Dwi ErnawatiCzech J. Food Sci., 2025, 43(4):263-273 | DOI: 10.17221/235/2024-CJFS Snails, which are high in protein, have the potential to be developed as a flavour enhancer through the hydrolysis process. However, consumer acceptance of the flavour enhancer needs to be fully evaluated. The aim of this study was to determine the consumer acceptance of a snail protein hydrolysate from different snail species (golden apple, apple, and freshwater) and at different hydrolysis durations (3, 6, and 9 h), and to identify the drivers of liking of snail protein hydrolysates through descriptive profiling using Rate-All-That-Apply (RATA) method and consumer testing using Hedonic test. The RATA intensity data were subjected to analysis using analysis of variance, followed by a Tukey's post hoc test (P < 0.05). Furthermore, the sensory profile data were analysed using principal component analysis and preference mapping. Snail species and hydrolysis time influenced the sensory profile of snail protein hydrolysate, with the longer hydrolysis time being the most liked. The most liked flavour enhancer derived from golden apple snail with 9 h hydrolysis time had a strong savoury aroma, salty taste, umami taste, lingering mouthfeel, and yellow colour. Additionally, it exhibited a moderate intensity of seafood aroma, a garlic taste, an umami aftertaste, a liquid mouthfeel, and a salty aftertaste. However, it had a low intensity of bitter aftertaste and burnt taste, and a very low intensity of sweet aroma, sweet taste, bitter taste, and bland taste. Thus, these findings highlight the importance of evaluating the efficacy of flavour enhancers and facilitate the identification of the optimal snail species and hydrolysis time according to consumer preference. |
Effect of microbial transglutaminase and banana peel powder on the structure and oxidative potentiality of camel milk yogurt during cold storageOriginal PaperRehab S. Alsulami, Elfadil E. Babiker, Isam A. Mohamed Ahmed, Tawfiq S. Alsulami, Fahad Y. Al-JuhaimiCzech J. Food Sci., 2025, 43(5):365-375 | DOI: 10.17221/100/2025-CJFS The current investigation was done to study the impact of adding banana peel powder (BPP) and treatment with microbial transglutaminase (MTGase) on the structure, physicochemical, antioxidant, and sensory qualities of yoghurt prepared from camel milk powder during cold storage. BPP analysis showed that it contained high amounts of total phenolics, which were accompanied by strong antioxidant activity. When employed alone or with BPP, MTGase polymerises yoghurt proteins, forming a structure with a high molecular weight above the stacking gel and lacking pores. The levels of protein, total phenolic compounds (TPC), total solids, ash, and antioxidant activity (DPPH – 2,2-diphenyl-1-picrylhydrazyl) increased after the addition of BPP to yoghurt, even after 21 days of storage. Adding BPP to yoghurt initially decreased thiobarbituric acid reactive substances (TBARS) levels, which then increased with the storage time. Yoghurt made with BPP and treated with MTGase was more stable with enhanced physicochemical, antioxidant, and sensory qualities during storage when compared to yoghurt made with MTGase alone. The results of the study demonstrated that MTGase is capable of cross-linking yoghurt proteins and that BPP can be applied as a stabiliser, antioxidant, and functional component in yoghurt production. |
Soil nutrient contents in a long-term field experiment following the suspension of phosphorus and potassium fertilisationOriginal PaperGabriela Mühlbachová, Helena Kusá, Pavel Rù¾ek, Martin Ká¹, Radek VaveraPlant Soil Environ., 2025, 71(11):770-781 | DOI: 10.17221/310/2025-PSE The effect of mineral, organic (manure or straw + intercrop) and combined fertilisation on the development of soil nutrient contents over time and their mutual ratios was evaluated in a long-term field trial, IOSDV (established in 1984 at two sites), differing in the soil-climatic conditions. Three cropping cycles, from 2016 to 2018, 2019 to 2021, and 2022 to 2024, were studied in the following crop rotation: winter wheat-winter barley-root crop (sugar beet at Ivanovice na Hané and potatoes at Lukavec). Potassium and phosphorus in mineral fertilisers have not been applied since the year 2020 due to their high content found in soils after dry years with low yields. Consequently, their content decreased, most in the third rotation, both by the Mehlich 3 method and especially the exchange fraction extractable with NH4-acetate (Ivanovice: P 5–14%, 32–40% and K up to 12%, 9–20% determined by Mehlich 3 and NH4-acetate, respectively; Lukavec: Mehlich 3 – P increase: 5–16%, K decrease: 0–8%; NH4- acetate decrease – P: 10–13%, K 8–23%). The mutual ratio of nutrients equivalents K : Mg : Ca was lower than required values 1 : 2–3 : 10–15 at both sites and all studied treatments, however a slight increase was observed during the studied period, above all in system with only mineral fertilisation (Ivanovice: K : Mg : Ca from 1 : 1.2 : 5.6 to 1 : 1.4 : 6.8, Lukavec: from 1 : 1.0 : 7.7 to 1 : 1.0 : 9.6). A correctly balanced ratio of nutrients in the soil is important for maintaining soil fertility. In this long-term field experiment, the increase in nutrient levels in soils over reasonable levels was observed, highlighting the necessity of regular nutrient testing in agricultural soils, especially when multiple types of fertilisers are used simultaneously. |
Presumed atypical cor triatriatum dexter in a geriatric dog: A case reportCase ReportY Jeong, D Kwon, K Park, K Lee, H YoonVet Med - Czech, 2026, 71(1):27-34 | DOI: 10.17221/50/2025-VETMED Membranous structures within the right atrium (RA), such as the Chiari network, eustachian valve, Thebesian valve, and cor triatriatum dexter (CTD), can present with overlapping imaging features and complicated diagnoses. A 17-year-old male Maltese presented with a cardiac murmur, cough, anorexia, and exercise intolerance. Echocardiography revealed a mobile membrane in the RA, dividing it into two chambers. Computed tomography (CT) confirmed this finding, demonstrating differential attenuation of contrast between the chambers. The vascular connections and anatomical relationships of the structure differed from those of previously reported CTD types. Although other sinus venosus valve remnants were considered, the findings supported a provisional diagnosis of atypical CTD. This case highlights the utility of multimodal imaging for the characterisation of right atrial membranous structures. In human medicine, a detailed anatomical comparison of sinus venosus valve remnants aids in the differential diagnosis. Applying a similar approach in veterinary medicine, especially in geriatric patients, may improve diagnostic accuracy. Comprehensive imaging evaluations, including echocardiography and CT, are recommended to assess RA membranes that may be misinterpreted as variants of the CTD or other embryological remnants. |
The impact of pre-dry-off weather patterns on subsequent lactation udder health in dairy cows, and their comparison with milkability, milk quality and udder health parameters – A pilot studyOriginal PaperMatú¹ Ga¹parík, Jaromír Ducháèek, Ludìk Stádník, Radim Codl, Iveta Szencziová, Kateøina Cihláøová, Nikola Mare¹ováCzech J. Anim. Sci., 2026, 71(1):21-29 | DOI: 10.17221/169/2025-CJAS
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Assessment of mineral nutrients and risk elements in plants growing on soils polluted by magnesite emissionsOriginal PaperMargita Kuklová, Ján Kukla, Jana Luptáková, Franti¹ek Hnilièka, Tomá¹ RýglPlant Soil Environ., 2026, 72(2):77-86 | DOI: 10.17221/443/2025-PSE Changes in the content of mineral nutrients (Ca, Mg, K, Na) and risk elements (Mn, Cd) in the assimilatory organs of selected plant species were studied along the altitudinal gradient of A‒D zones polluted by alkaline emissions from the magnesite factory Lubeník (Slovak Republic). Multivariate statistical analysis and comparison with background values in other studies demonstrate persistent intoxication of some plants by Mg (all study plants), K (Lactuca saligna, Dryopteris filix-mas), Mn (Quercus polycarpa, Carpinus betulus, Betula pendula, Lactuca saligna) and Cd (Quercus polycarpa, Carpinus betulus, Betula pendula, Lactuca saligna). Overall, Lactuca saligna accumulated the highest amounts of Mg, Cd, Na and K near the magnesite plant, suggesting its potential as an effective bioindicator of elemental pollution. Unbalanced Ca/Mg ratios, lower than 1, were recorded predominantly in all plant species sampled near the magnesite plant; unbalanced K/(Mg + Ca) ratios were predominantly in woody species. |
