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The subject of this study is to point out the issue of various impacts of musculoskeletal disorders in chainsaw operators and their impact on human health. The first goal was to find out whether the body mass index is responsible for excessive stress to individual parts of the body of chainsaw operators at work. The second goal was to determine whether the excessive load to the individual body parts is affected by the time of working with the chainsaw. Questionnaires created for the fulfilment of the goals were distributed to professional forest loggers working in the Czech Republic, which were filled in by 161 of them. Questions in the first part of the questionnaire were formulated in such a way that answers to them would clearly identify information on the individual respondents. The second part of the questionnaire was focused on the assessment of the load upon 12 body parts of the operators (each part being split into the right and left sides). The results of the statistical analysis showed that neither the body mass index (BMI) nor the length of time working with the chainsaw affect the load on the individual parts of the body of the chainsaw operators.

As a consequence of climate change and damage to coniferous forests, European beech (Fagus sylvatica L.) is the preferred plant species for forest restoration in Central Europe. European beech is generally regarded as pest-resistant. However, its vulnerability to secondary pests, for instance, gall-forming midges, may increase with environmental stress such as long drought periods. We analysed the abundance of two gall-forming insects, Mikiola fagi and Hartigiola annulipes, on European beech at 26 forest sites across the Czech Republic, spanning diverse climatic and environmental conditions, using generalised linear mixed models to evaluate the effects of abiotic factors and host tree characteristics. The results revealed that M. fagi was more abundant on younger trees, in stands with lower canopy closure, and under warmer spring conditions. In contrast, the abundance of H. annulipes declined in drought-affected areas. These patterns demonstrate species-specific responses of gall midges to host tree characteristics and climatic variables, suggesting that climate change may favour higher M. fagi abundance. Accordingly, our findings support the establishment of young beech stands under higher canopy closure, for example, beneath the shading of mature trees.

The testing and evaluation of native populations of perennial ryegrasses (Lolium perenne L.) collected from the Republic of Srpska, Bosnia and Herzegovina were conducted from 2020 to 2022 at experimental fields and laboratories of the Institute for Genetic Resources and the Agricultural Institute of the Republic of Srpska in Banja Luka. Six native populations (labelled G1–G6) and two cultivars, Esquire and Tivoli (labelled G7 and G8), were analysed in this study. The following traits were examined: the plant height (cm), number of generative stems per plant, inflorescence length (cm), number of spikes per inflorescence, seed mass per inflorescence (g), and seed yield per plant (g). The results showed that all the native populations exhibited higher average plant heights compared to the Esquire standard (G7). Population G2 had the highest average number of spikes per inflorescence (26.8), while population G4 achieved the greatest average inflorescence length (27.7 cm). Statistically significant differences in the inflorescence length were observed between populations G2, G3, G4, and G6, compared to the standards G7 and G8 (Esquire and Tivoli). The genotype and year had a significant impact on the seed yield per plant. The average yield ranged from 32.4 g (G7) to 53.4 g (G4). The seed yield per plant showed a strong positive correlation with the number of generative stems per plant, the inflorescence length, and the number of spikes per inflorescence. The native populations G4 and G5 achieved significantly higher seed yields per plant compared to the other populations and may serve as highly valuable material for breeding programmes.

Malus domestica is one of the world’s most important deciduous fruit trees. Over a four-year period (2017–2020), temporal variations in flowering were evaluated in ‘Honeycrisp’ apple trees on eight rootstocks (G.30, G.969, G.202, G.41, G.11, M.9T337, M.26 EMLA and V.6) planted in 2014 in Chihuahua, México. Among the variables evaluated were the probability of late-spring frost, winter chill units, growing degree days, flowering period, foliar nutrient concentrations, trunk cross-sectional area, number and weight of fruit per tree, and production efficiency. Significantly different chill unit accumulations occurred over the four years, with values falling between 974 and 1 415, where for the latter value, the start of flower opening was earlier, but there was a higher risk of damage by temperatures ≤ –2 °C. There was no effect of rootstock on the time of onset and end of flowering. The most productive combinations were ‘Honeycrisp’ on G.969, G.11 and V.6 with yield estimates of 35 300, 34 200 and 33 600 kg/ha, respectively. The commercial production of ‘Honeycrisp’ apple trees requires the evaluation of their agronomic performance with different rootstocks. Flowering is particularly important since this phenological stage is so closely linked to productivity and is strongly affected by variations in winter temperatures.

This study aimed to investigate the effects of phytase supplementation in phosphorus (P)-reduced diet on the productive performance, egg quality, calcium (Ca) utilisation, and blood profile of Japanese quails. Phytase breaks down phytic acid; thereby, increasing the availability of its bound nutrients, enabling poultry to hydrolyse and utilise these nutrients effectively. A total of 480 female Japanese quails (Coturnix coturnix japonica; 28-day-old and average body weight = 94.3 ± 5.7 g) were assigned to four dietary regimens, each consisting of six replicates of 20 birds. The control diet (T0) contained 0.5% nonphytate P. The other experimental diets included: T1 = comprising 0.4% nonphytate P supplemented with 0.1% phytase equivalent to 500 Phytase Unit (FTU)/kg; T2 = comprising 0.3% nonphytate P supplemented with 0.15% phytase equivalent to 750 FTU/kg; and T3 = comprising 0.2% nonphytate P supplemented with 0.2% phytase equivalent to 1 000 FTU/kg. The addition of phytase to P-reduced diets did not affect the overall productive performance in quails. Moreover, yolk weight increased by 3.04% to 10.5% (P = 0.01) and the haugh unit increased by 0.56% to 1.11% (P = 0.04) compared to the control, whereas other quality traits, such as albumen weight, albumen and yolk indices, and eggshell weight, thickness, and breaking strength, remained unaffected following the supplementation of phytase in the P-reduced diet. Additionally, an increase in Ca output in eggshells (5.26% to 15.79%; P = 0.14) and the ratio of Ca in eggshells to Ca intake (5.59% to 16.47%; P = 0.49) correlated with the increase in blood Ca levels in the quails on the P-reduced diet (P = 0.16). In conclusion, the addition of phytase to P-reduced diets has proven effective in maintaining the laying performance, egg quality, and blood biochemical profiles of Japanese quails.

Forest transformation commonly occurs in subtropical areas due to extensive human disturbance. However, we know little about how forest transformation may affect the soil water-holding capacity. Here, we evaluated the effects of forest transformation from natural forests to secondary forests, Castanopsis carlesii plantations, and Cunninghamia lanceolata plantations on the soil water-holding capacity, including the soil water content (SWC), maximum water holding rate (R_t), capillary holding rate (R_c), and non-capillary water holding rate (R_n), and assessed the influences of soil properties and stand characteristics on the forest transformation effects. The results showed that (i) the soil water-holding capacity in secondary forests increased significantly (SWC: 27.3%; R_t: 50.9%; R_c: 36.9%; R_n: 14.0%), but decreased in the Cunninghamia lanceolata plantations (SWC: 24.6%; R_t: 47.0%; R_c: 34.0%; R_n: 13.0%), compared to the nature forests (SWC: 26.0%; R_t: 48.3%; R_c: 34.9%; R_n: 13.4%); (ii) the soil water-holding capacity was positively correlated with the soil porosity, soil total nitrogen concentration, stand density, but negatively influenced by the soil bulk density and diameter at breast height (DBH); and (iii) the stand density, DBH and litterfall amount were the major factors regulating the soil water-holding capacity after the forest transformation. Overall, these results indicated that the soil water-holding capacity would be strongly altered by the forest transformation, but it depends on the soil properties before the transformation and the characteristics of the transformed forests. Our findings will help to better understand the functions of forests in water source conservation under the pressures of human disturbances and environmental changes.

The study tested the effect of daily feed ration (DFR: 0.5%, 0.75%, 1.0%, 1.25%, and 1.5% of fish biomass) on juvenile pikeperch (Sander lucioperca) with an initial body weight of 21.5 ± 5.1 g and total length of 144.5 ± 8.5 mm. The pikeperch were fed floating feed at 8-hour intervals for a duration of 84 days. By the end of the experiment, the group fed DFR of 1.5% exhibited the highest body weight (51.5 ± 16.1 g) and total length (188.2 ± 17.8 mm). The weight heterogeneity, measured as the coefficient of variation (CV), ranged between 30.29 and 33.24%. The specific heterogeneity rate (SHR) ranged from 304.44 to 334.94‰/day. The group with DFR of 1.0% exhibited the highest degree of heterogeneity. Minor fin erosion was observed in the caudal fin by the end of the experiment. No significant differences were revealed in selected biochemical parameters indicating the liver, spleen, and intestinal function. All the fish tested were adequately fed, being provided sufficient nutrients for the proper growth of pikeperch. The DFR of 1.5% was evaluated as the most favourable. This amount of feed supported a higher number of values for Fulton’s coefficient (FC), specific growth rate (SGR), thermal growth rate (TGR), fish weight heterogeneity, growth of total fish biomass (BG), and optimum level of biochemical parameters in blood plasma.

Scots pine (Pinus sylvestris L.) is one of the most widespread and economically important coniferous species in the Northern Hemisphere. However, its regeneration success has recently been increasingly affected by global climate change (GCC), particularly through increasing mortality. This research aimed to evaluate the early establishment success of 30 400 bare-root and containerised Scots pine seedlings and saplings of different height classes, planted at various times on acidic and gleyed sites (479–610 m a.s.l.) across four locations in the Czech Republic. On acidic sites, seedlings exhibited 16% higher height growth and 11% lower mortality compared to gleyed sites. Containerised planting stock achieved significantly (P < 0.05) greater height growth (by 83%) and lower mortality (by 36%) than bare-root stock. For containerised material, spring plantings showed superior growth and vitality compared to autumn plantings. Planting stock height class had a stronger influence on height growth than on mortality. Based on the results, planting containerised saplings of 26–35 cm or 36–50 cm height in April is recommended for optimal establishment success of Scots pine under GCC.

Strawberry mild yellow edge virus (SMYEV) was detected in 116 samples out of 423 collected from strawberry plants grown in commercial and experimental plantations in seven provinces of Poland. The number of samples infected with strawberry mottle virus (SMoV) accounted for 84.6% of the 26 SMYEV-positive samples selected for sequence analysis. The nucleotide sequence similarity of the coat protein (CP) gene of 26 selected SMYEV isolates ranged from 84.8% to 100%, and 81.4–99.5% identity was found between these isolates and 48 SMYEV strains from different countries. The CP region's phylogenetic analysis showed that most isolates from Poland clustered within group I (type D74). In contrast, Talis and 3233CL isolates represented group III (type MY18), and the San isolate was clustered in group V (type ABY1-01). Recombination analysis of the CP gene sequences detected two possible recombination events. One was noticed in the Argentinian strain 53, which formed group III with isolates from Chile, and Polish isolates Talis and 3233CL. Another was identified in the Chinese strain sy02 sequence with evidence of the same recombination event in Canadian strains, and the Polish isolate San (V group). Leaf epinasty, mottling, and yellowing of the young leaves and dieback of the older leaves were observed on Fragaria vesca 'Alpine' and 'EMC' indicator plants grafted with leaves of strawberry plants co-infected with SMYEV and SMoV. A single infection with SMYEV induced milder symptoms based on these indicators. 

Developing natural herbicides offers a potential solution to mitigate the drawbacks associated with synthetic pesticides used in an excessive quantity to safeguard agricultural crops. In this study, essential oils extracted via hydrodistillation from Pinus ponderosa needles were investigated for their chemical composition and phytotoxic activity. Gas chromatography with mass spectrometry detection (GC/MS) identified twenty-three constituents, constituting 93.87% of the total oil. The predominant components were oxygenated monoterpenes (64.66%), with α-pinene (37.78%), β-pinene (24.32%), and sesquiterpenes hydrocarbons, particularly germacrene-D (7.26%). The phytotoxic effects of P. ponderosa essential oil were tested on Phalaris canariensis L., Trifolium campestre Schreb., and Sinapis arvensis L. The essential oil exhibited a significant inhibitory effect on seed germination and seedling growth in a dose-dependent manner. A low concentration of essential oil reduced the germination and seedling growth of all tested weeds. Additionally, the essential oil treatment impacted malondialdehyde content and electrolyte leakage in the seedlings. These preliminary findings suggest that essential oils from forest trees, particularly Pinus ponderosa, could serve as an eco-friendly alternative to chemical herbicides. This approach may contribute to addressing the challenges associated with synthetic pesticides while promoting sustainable and environmentally friendly agricultural practices.

Nitrogen management and irrigation methods play crucial roles in determining rice’s grain yield and quality (Oryza sativa L.). However, limited knowledge exists on how interactions between nitrogen forms and irrigation regimes regulate starch-metabolising enzyme activity to influence rice yield and quality. A soil-growth experiment was conducted using a high-lodging-resistance rice cultivar under three irrigation methods, namely, submerged irrigation (0 kPa), alternate wetting and moderate drying (−20 kPa), and alternate wetting and severe drying (−40 kPa), as well as three nitrogen forms, namely, ammonium nitrogen (NH₄⁺-N), mixed ammonium + nitrate (50 : 50), hereafter denoted as 50 : 50, and nitrate nitrogen (NO₃^–-N). Results indicated that compared with the other treatments, alternate wetting and moderate drying interacted with 50 : 50 treatment, resulting in the following: improved grain yield by 11.7–21.0%, milling, appearance, eating and cooking, and nutritional qualities including milled-rice and gel consistency; and decreased chalky rice, chalky size, chalky degree, amylose content, and protein content by 20.0–23.1, 29.6–33.3, 44.1–48.5, 6.2–9.6 and 10.1–13.9%, respectively. The activities of adenosine phosphate glucose pyrophosphorylase (AGPase), starch synthase (SS), starch-branching enzyme (SBE), and adenosine triphosphate (ATP) enzyme in the grains also improved, with an increase of 20.0–35.0, 11.8–20.0, 13.6–26.3 and 21.2–39.6%, respectively. Conversely, severe drying and NO₃^–-N treatment negatively impacted grain yield and quality due primarily to decreased SS activity in grains under each irrigation method. Correlation analysis showed that starch-metabolising enzyme (AGPase, SS and SBE) activity at 14 days after anthesis (DAA) and 28 DAA exhibited a positive correlation with grain yield, milling quality and gel consistency, whereas negatively correlated with appearance and nutritional qualities. In summary, the adoption of alternate wetting and moderate drying and 50 : 50 interaction treatment can synergistically boost grain yield by increasing the filled-grain rate and 1 000-grain weight and enhance grain quality of rice by upregulating the activities of starch-metabolising enzyme activity.

To evaluate the effects of organic fertilizer and microbial agents on soil water and salt distribution, microorganisms, and crop yield on coastal saline-alkaline land, eight treatments were established, i.e., two maize varieties (Dajing Jiu 26 (J) and Quchen Jiu (Q)) and four organic fertilizer and microbial agent application methods (no organic fertilizer or microbial agent application (O₀M₀), single organic fertilizer application (O₁M₀), single microbial agent application (O₀M₁), and combined organic fertilizer and microbial agent application (O₁M₁)). The soil water content in the 40–50 cm soil layer under JO₁M₁ was 3.35% greater than that under JO₀M₀ at the mature stage. The soil organic carbon (SOC) and soil total nitrogen (STN) in the 0–10 cm soil layer under JO₁M₁ were 16.69% and 21.37% greater, respectively, than those under JO₀M₀ at the jointing stage. The actinomycete content was 58.79% greater in QO₁M₁ than in QO₀M₀. The urease activity was greater in O₁M₀ than in the other management practices. Compared with that in JO₀M₀ and QO₀M₀, the alkaline phosphatase activity in JO₁M₁ and QO₁M₁ was 47.36% and 33.97% higher, respectively. Compared with those of JO₀M₀, the catalase activity and sucrase activity of JO₁M₁ were 57.62% and 22.78% higher, respectively. Compared with JO₀M₀ and QO₀M₀, JO₁M₁ and QO₁M₁ increased the grain yield by 20.69% and 16.42%, respectively, and increased the biomass by 23.36% and 26.45%, respectively. In summary, organic fertilizer and microbial agents provide a scientific model for the rational use of saline soils and the development of their potential.

To better explore the effects of adding phytosterol (PS) microcapsules to feed on pig growth performance, nutrient apparent digestibility, and serum biochemical indicators, 200 healthy ternary hybrid Duroc × Landrace × Yorkshire piglets with an average initial weight of 7.53 ± 0.57 kg) were used as experimental subjects, and randomly divided into five groups with four replicates in each group. The control group of pigs was fed the basic diet, while the experimental group received diets supplemented with different PS levels. They were divided into experimental groups 1 to 4 (100 mg/kg to 5 502 mg/kg) according to different dosage added components. The duration of the experiment was 54 days. The results showed that the total triglyceride (TG) index and diarrhoea status in the experimental group were significantly improved compared to the control group (P < 0.05). The increase in PS addition levels was more significant in the change of pig average daily gain (ADG) (P < 0.05), and the difference in average daily weight gain and feed conversion ratio between PS-400 and PS-550 was significant (P < 0.05). Compared with the control group, the experimental group showed significant differences in crude fibre (CF), ether extract (EE), dry matter (DM), organic matter (OM), average digestible energy (ADE), metabolizable energy, xylan, and calcium (P < 0.05), with a maximum increase of 7.02% in calcium content. The addition of PS can be effective in making the experimental and control groups show a significant difference in value changes in high-density cholesterol (HDL-C) and low-density cholesterol (LDL-C) indicators (P < 0.05), while no significant changes were revealed in the other indicators (P > 0.05). From the above, plant sterol microcapsules can effectively improve pig growth performance and nutrient apparent digestibility, and improve their blood lipid status.

Recently, protected cultivation of bananas under subtropical conditions has gained popularity due to the impact of global climate change. Several factors influence the yield and quality of banana cultivation. Among these factors, variety plays an important role along with cultural practices. This study aimed to determine the performance of ‘Azman’, ‘Bango’, ‘Bonus’, ‘Grand Nain’, ‘Lider’, and ‘Paşa’ banana varieties in terms of morphological and yield characteristics under protected cultivation, as well as to elucidate the relationship between yield and factors affecting yield. The research was conducted between 2020 and 2022 in the Manavgat district of Antalya. The research findings showed that each morphological parameter examined varied significantly. However, the ‘Bango’ variety exhibited superior results in terms of finger circumference (13.23 cm), finger length (21.08 cm), bunch weight (39.33 kg), and yield per hectare (72 t/ha). Moderate to high-level phenotypic correlations were found between bunch weight and pseudo stem girth, pseudo stem height, number of fingers, finger weight, finger circumference, and finger length, with values of 0.544, 0.478, 0.326, 0.669, 0.581, and 0.543, respectively. Positive and significant phenotypic correlations were determined between finger length affecting export quality, bunch weight, finger weight, and finger circumference, with values of 0.543, 0.799, and 0.625, respectively. It can be concluded that the ‘Bango’ variety exhibited better results as compared to other varieties in terms of both yield and quality criteria. Additionally, positive phenotypic correlations were observed between bunch weight (which directly influences yield) and finger length (which affects export quality), as well as various morphological and pomological characteristics.

Anthocyanins have received an increased attention not only because of its antioxidant activity; but because fortification of food products by minerals is important due to the lack of some minerals in population. The addition of these minerals can affect the sensorial and nutritional composition of food. The influence of calcium fortification on anthocyanins and colour changes in strawberry puree were assessed by accelerated storage test. The quantification of anthocyanins was performed by high-performance liquid chromatography with diode-array detection (HPLC-DAD) and colour changes were measured spectrophotometrically (CIE L* – lightness, a* – redness, b* – yellowness). The kinetical parameters (velocity constants and activation energies) were calculated. The activation energies of degradation of anthocyanins were calculated as pelargonidin-3-glucoside (26.24 ± 0.57, 21.18 ± 1.07, and 24.53 ± 1.33 kJ·mol^–1), cyanidin-3-glucoside (16.10 ± 0.96, 11.61 ± 0.74, and 13.34 ± 1.72 kJ·mol^–1), and pelargonidin-3-rutinoside (8.91 ± 0.17, 7.39 ± 0.98, and 8.23 ± 1.72 kJ·mol^–1) of the control sample, calcium carbonate and calcium citrate respectively. The results showed that the addition of calcium salt had a statistically significant (P ≤ 0.05) effect on the degradation of anthocyanins.

The clonal propagation of guava (Psidium guajava L.) is essential to avoid segregation, and its multiplication through cuttings is the easiest and cheapest way to raise a nursery, as other propagation techniques are laborious and need expertise. The current study was planned to evaluate the best rooting stimulators and rooting substrates for guava nursery propagation. In this experiment, the guava-prepared cuttings were treated with rooting stimulators, indole butyric acid (IBA) and paclobutrazol (PB) with concentrations (0, 200, 400, and 600 ppm) and were planted in independent rooting substrates (sand, silt and topsoil) under polytunnels. The results revealed that the highest number of roots, root length and root weight were achieved in cuttings planted in the sand with IBA 400 ppm concentration. In contrast, the highest survival percentage (50%) was obtained in silt substrate with a similar concentration of IBA. However, sand’s lowest and highest concentrations of both rooting hormones produced zero plants. In shooting capacity, maximum leaves (13.6) and sprouts (3.76) were obtained using IBA 400 ppm in silt substrate. The highest sprouting length (6.80 cm) and cutting height (26.2 cm) were attained with sand using PB 200 ppm. In the overall comparison, rooting stimulator IBA 400 ppm and rooting substrate silt performed better than other rooting stimulators and rooting substrates.

This research work evaluated some engineering properties of breadfruit starch flour following standard procedures with the aim of providing engineering data that would help its usage in food processed industries. Starch was extracted from matured breadfruit fruits, modified using acid and engineering properties were examined following standard procedures. Results of moisture contents, loose and packed bulk densities, density ratio and porosity were ranged from 8.23–9.13% dry basis (DB); 0.44–0.51 g·mL–1; 0.60–0.66 g·mL–1; 70.01–80.14% and 19.87–29.99%, respectively. Similarly, Carr index values ranged from 19.44–30.61% and Hausner ratio values ranged from 1.25–1.43, indicating that the flour samples investigated had poor (native), fair (modified) and good (potato) flow properties, using Carr index standard. Amylose, amylopectin and amylose-amylopectin ratio contents values ranged from 21.52–29.77%; 70.23–78.48% and 0.28–0.42%, respectively. Also, the flour samples thermal properties values using Differential Scanning Calorimeter (DSC) ranged from 106.4–129.09 °C; 31.93–78.36 °C; 106.2–175.75 °C; 214.90–278.6 J·g–1; 35.5–148.82 °C; 2.26–10.95 J·g–1·K–1; 0.305–3.0 (×10–6) W·m–1· °C–1, and 0.095–1.32 (×10–6) m2·s–1 for peak temperature, onset temperature, end temperature, enthalpy, temperature range, specific heat capacity, thermal conductivity and thermal diffusivity, respectively. Viscosity values as influenced by concentration and temperature ranged from 25.8–149 mPas for native starch and 41.9–109.6 mPas for modified starches. Hence, this research work provides engineering data that would help in process control, process design and bulk handling of breadfruit starch flour so as to promote its usage in food process industries.

Due to the earthworms’ implications in nutrient cycles through their burrowing and casting activity, earthworms are worth considering when urban biogeochemical cycles are analysed. Several chemical parameters and their relationships were analysed in earthworm casts of two urban parks, namely the pH, total organic carbon (TOC), total nitrogen (Nt), plant available phosphorus (P), plant available potassium (K), and calcium water soluble (Ca). It was statistically significantly found that the TOC, Nt, P and K are reciprocally determined in the earthworm casts: 74.4% of the pH variability is co-determined by the N, P, K, TOC, and Ca contents; 95.9% of the Nt variability is co-determined by the pH, P, K, TOC, and Ca contents; 95.4% of the P variability is co-determined by the pH, N, K, TOC, and Ca contents; 94.5% of the K variability is co-determined by the pH, N, P, TOC, and Ca contents; 86.6% of the TOC variability is co-determined by the pH, N, P, K, and Ca contents. This study revealed the complexity of the chemical relationships inside earthworm casts, their reciprocal dependencies, and highlighted the complexity of the earthworms’ contribution to biogeochemical cycles in urban areas. Our findings propose earthworms as indicators of the integrative conservation management of urban ecosystems.

The objective of the study is to determine the impact of shock from climate change, such as drought and flood, on various technology adoptions in rice production in Vietnam. This study utilised VARHS (The Vietnam Access to Resources Household Survey) panel data from 2012 to 2018 and employed regression analysis following the application of propensity score matching (PSM) to address potential selection bias caused by drought and flood shocks. The findings indicated that households tend to adopt the improved variety of rice and organic fertilisers since households suffer the shock from the drought. Another finding showed that households used more chemical fertiliser and reduced the probability of using improved seeds in rice production since the household was affected by floods. Based on the findings, policies should prioritise promoting and implementing environmentally friendly farming methods that are customised to address specific climate-related difficulties.

Oat (Avena sativa Linnaeus) has distinctive multifunctional characteristics and nutritional profile, as well as a large amount of oat-processing by-product comprises hulls, which contain lipids and other nutrients. In this study, the lipid content and fatty acid (FA) profiles of six naked oat varieties (Kamil, Marco Polo, Oliver, Patrik, Santini, and Saul), two hulled oat varieties (Atego and Korok), and their dehulled grains and hulls were analysed. The findings of the study demonstrated that the lipid content varied from 4.14 g·100 g^–1 dry matter (DM) (Santini) to 6.68 g·100 g^–1 DM (Kamil) in naked oats; 3.61 g·100 g^–1 DM in Atego and 3.47 g·100 g^–1 DM in Korok with hull; 0.70 g·100 g^–1 DM in Atego hull and 0.71 g·100 g^–1 DM in Korok hull. Dehulled oats had a higher lipid content than hulled oats. Linoleic and oleic acids were the predominant FAs in analysed samples. Oat hulls contained maximum amounts of saturated FAs (SFAs) (26% in Korok and 25.6% in Atego). Elimination of hulls raised the amount of linoleic acid and decreased the amount of oleic acid. Oat hull contained the least amount of linoleic acid and the highest amount of C20 : 0 (eicosanoic acid) and C22 : 0 (docosanoic acid). Oats are a significant source of lipids, predominantly comprising unsaturated fatty acids (UFAs). Moreover, oat hulls contribute to the lipid content although their FA composition, with higher palmitic acid and lower linoleic acid levels, differs from that of naked, hulled, and dehulled oats.

Polyphenols and carotenoids are important bioactive compounds in potato tuber. Knowledge of these features makes it possible to select cultivars for cultivation, consumption, and processing with desirable properties. For this purpose, the content of polyphenols and carotenoids in edible potato tubers from different countries was analysed. The research material consisted of eight cultivars, including one light yellow – Eurostar, two with red flesh – Rote Emmalie and Herbie 26 and five cultivars with purple flesh – Provita, Salad Blue, Blue Annelise, Vitelotte Noire and Bora Valley. They were cultivated in a three-year, single-factor field experiment. The content of polyphenols in the analysed cultivars ranged from 165.0 to 283.8 mg/kg of fresh weight and depended on the cultivar, year of research and cultivar-year interactions. The highest average amounts of these compounds were noted in the Herbie 26, Rote Emmalie and Vitelotte Noire cultivars. The content of carotenoids ranged from 1.122 to 3.173 mg/kg of fresh weight and depended significantly on the cultivar, weather conditions in the years of the study and the interaction of cultivars with years. The highest amounts of carotenoids were determined in the purple-fleshed cultivars Blaue Annelise and Vitelotte Noire and the red-fleshed cultivar Rote Emmalie.

Mastitis, mainly caused by contagious bacteria, is an important disease in dairy goat production, especially subclinical mastitis. The aim of the research was to study the relationship between somatic cell count (SCC) and presence of mastitis pathogens in goat milk during the period 2022–2024. A total of 987 udder-half milk samples were obtained from 5 goat farms in Slovakia. The presence of pathogens was determined by bacterial cultivation and grown colonies were identified by MALDI-TOF MS at the species level. Fossomatic 7 was used to determine SCC. To evaluate the frequency distribution of the samples, the following groups of SCC were considered: SCC1 < 500 × 10³ cells/ml, SCC2 ≥ 500 < 1 000 × 10³ cells/ml, SCC3 ≥ 1 000 < 2 000 × 10³ cells/ml, SCC4 ≥ 2 000 × 10³ cells/ml. The individual milk sample were 42.2% in SCC1, 17.0% in SCC2, 16.7% and 24.0% in SCC3 and SCC4, respectively. Of the total number of samples, 23.0% were bacteriologically positive. The most frequently identified pathogens were Staphylococcus spp., especially non-aureus staphylococci and mammaliicocci (NASM). Among the NASM, Staphylococcus epidermidis (40.3%), Staphylococcus caprae (27.5%) and Staphylococcus simulans (10.4%) were the most prevalent species. Only 2 samples were positive for Staphylococcus aureus. A higher occurrence of pathogens was identified in SCC ≥ 1 000 × 10³ cells/ml (SCC3 and SCC4) compared to SCC < 1 000 × 10³ cells/ml (SCC1 and SCC2). However, approximately 34.8% of uninfected half udders with no bacteriological findings had SCC ≥ 1 000 × 10³ cells/ml. In conclusion, NASM were the most common bacterial causative agents of subclinical mastitis in goats and it is not easy to set a specific SCC for subclinical mastitis diagnosis as it is in dairy cows.

The aim of this research was to quantify the bioactive compounds; antioxidant, antibacterial, and antihypertensive capability of different cahuiche (Vaccinium leucanthum Schltdl.) extracts in vitro. Aqueous, ethanolic, and methanolic extracts were obtained from cahuiche berries (Vaccinium leucanthum Schltdl.). Total phenols, flavonoids (quercetin and catechin), and anthocyanins contents were determined, and antioxidant activity was also determined using ABTS [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic) acid] and DPPH (2,2-diphenyl-1-picrylhydrazyl), antibacterial activity, and antihypertensive potential, using angiotensin I-converting enzyme (ACE) inhibitor activity (%) in vitro. Methanolic extract showed the highest total phenols (1187.88 ± 87.48 mg gallic acid equivalents), catechin (372.17 ± 35.23 mg catechin equivalents), anthocyanins (578.89 ± 38.5 mg cyanidin-3-glucoside equivalents), ABTS (98.26 ± 0.00%) and DPPH (44.06 ± 3.95%) antioxidant activity, and ACE inhibitor activity of 80.20 ± 2.46%. All extracts presented antibacterial activity against Staphylococcus aureus (ATCC 25923) and Listeria monocytogenes (ATCC 19115) with inhibitor zones of > 11 and 13 mm, respectively. Cahuiche berries are potentially an ideal food or functional ingredient due to their biological activities as natural antihypertensive and antibacterial agent, and high bioactive compound contents.

The present study aimed to determine the chemical composition and in vitro antimicrobial potential for the first time of essential oils (EOs) from six cultivars (Druzhba, Raya, Hebar, Hemus, Yubileina, Sevtapolis) of lavender (Lavandula angustifolia Mill.) cultivated in Türkiye (Tekirdağ) against a panel of pathogenic and non-pathogenic microorganisms. The chemical composition of EOs, analysed using gas chromatography/mass spectrometry (GC/MS), revealed 34 different components in the six cultivars. The results indicate that the main major constituents of all EOs were monoterpenoid linalool (47.60–64.13%) and linalyl acetate (12.92–26.08%). Based on principal component analysis (PCA) cvs. Druzhba, Raya and Hebar were situated in the centre right quadrant of the plot and were characterised by linalool and linalyl acetate in subgroup one. The results of antimicrobial assays revealed that lavender EOs were active against all strains of bacteria tested. For bacteria, the strongest activity of cv. Hemus was observed against Enterococcus faecalis (IZ = 19 ± 0.10 mm, MIC = MMC = 6.25 (µg/mL), while the weakest potency was seen against the gram-negative Salmonella enterica (IZ = 21 ± 1.00 mm, MIC = MMC = 31.25 (µg/mL). Based on PCA, the first subgroup of cvs. Hebar and Raya was characterised by Lactobacillus rhamnosus, E. faecalis and Lactobacillus pentosus and was situated in the bottom right quadrant of the plot.

Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.

The environment is characterised by subtle and major mutations that cause changes in land use/land cover. Analysis of its dynamics and identification of vulnerable areas are critical to maintaining ecosystem services. The aim of this research is to quantify and qualify land cover dynamics over a 30-year period. It will also highlight forest degradation from a supervised classification of Landsat satellite imagery (L5 TM1987, L7 ETM+ 2000, and L8 OLI/TIRS 2019). The dynamics of land use/land cover were investigated by a maximum likelihood approach using geographic information system (GIS) and remote sensing (RS). Six major land use and land cover (LULC) types were mapped (build-up, agriculture, forest, clearing, matorral and olive cultivation). The classification reports made it possible to assess a reduction in forest cover (from 14 470.11 ha to 5 203.26 ha) and an increase in buildings (from 6 033.69 ha to 9 515.61 ha), and agricultural land (from 9 517.59 ha to 12 338.19 ha). The results were validated by a kappa coefficient of 0.93, 0.91, and 0.96, which showed that the model had successfully predicted LULC changes. We anticipate that the results will provide a basis for decision-making as well as a starting point for further in-depth studies in sustainable management and development of natural resources in the study region.

o uncover the regulatory metabolism of poly-glutamic acid (PGA) in protecting wheat crops against salt stress (SS) at the physiological level, we utilised hydroponic experiments to explore the roles of PGA in regulating the photosynthetic performance, water physiology, antioxidant metabolism and ion homeostasis of wheat seedlings exposed to SS for 10 days. The findings demonstrated that SS inhibited the photosynthetic performance of wheat seedlings. In contrast, different doses of PGA all improved the photosynthetic performance, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS decreased nonphotochemical quenching (q_N) by 26.3% and respectively increased photosynthetic rate (Pn), soil and plant analyser development (SPAD) value, maximum photochemical efficiency of photosystem II (PSII) (F_v/F_m), photochemical quenching (q_P) and actual photochemical efficiency of PSII (Y(II)) by 54.0, 27.8, 34.6, 42.4 and 25.8%. For water metabolism, SS destroyed the water balance of wheat seedlings. In contrast, different doses of PGA enhanced water balance, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS decreased leaf water saturation deficit (LWSD) by 35.5% and respectively increased leaf relative water content (LRWC), transpiration rate (T_r), stomatal conductance (g_s) and the contents of soluble sugars (SSS) and proline (Pro) by 15.9, 94.7, 37.5, 44.6 and 62.3%. For antioxidant metabolism, SS induced the peroxide damage to wheat seedlings. In contrast, different doses of PGA all mitigated the SS-induced peroxide damage, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS respectively decreased superoxide anion (O₂^–), hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents and electrolyte leakage (EL) by 39.1, 29.6, 46.2 and 36.3%, and respectively increased superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductases (DHAR) and monodehydroascorbate reductase (MDHAR) activities, and antioxidants ascorbic acid (AsA) and glutathione (GSH) contents by 69.2, 49.2, 77.8, 80.6, 109.5, 121.7, 104.5, 63.8 and 39.6%. Besides, SS destroyed the ion homeostasis of wheat seedlings. In contrast, different doses of PGA all maintained ion homeostasis, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS reduced Na+ content by 40.7% and respectively increased K⁺, Ca²⁺ and Mg²⁺ contents by 64.4, 82.6 and 105.6%, thereby respectively increasing K⁺/Na⁺, Ca²⁺/Na⁺ and Mg²⁺/Na⁺ ratios by 177.6, 209.4 and 244.8%. In the above ways, SS inhibited wheat height and biomass. In contrast, different doses of PGA all improved wheat height and biomass under SS, especially for 0.3% PGA. Compared with SS, 0.3% PGA plus SS, respectively, increased wheat height and biomass by 27.4% and 41.7%. In the above ways, PGA mitigated salt toxicity in wheat seedlings. The current findings implied that there was a potential for the use of PGA in real situations to improve wheat salt tolerance, especially for the 0.3% dose.

Optimum nutrition is essential for quality cut flower production and for improving soil health. The study aims to evaluate the interaction between bio-organic and inorganic nutrient sources in enhancing alstroemeria (Alstroemeria hybrida L.) cut flower production and soil health for sustainable cultivation practice. Randomised block methodology involving 20 treatment combinations of inorganic fertilisers (NPK) and biostimulants (Panchgavya and Jeevamrit) applied at varying concentrations. Significant outcomes emerged from the combined influence of inorganic and organic sources of nutrients. The application of the 100% recommended dose of fertiliser (RDF) with 75 mL of Panchgavya resulted in substantial improvements in alstroemeria growth parameters, including plant height (7.8%), early flowering (13.08%), flower diameter (20.03%) and the number of flowering stems (25.3%) over the control (100% RDF). Plant spread (24.1%) and number of florets/stem (26.6%) were improved with the application of 100% RDF with 50 mL of Panchgavya when compared with the control. Soil nutrient content, i.e. available nitrogen (11.5%), phosphorus (28.7%), potassium (13.8%) and microbial populations, i.e. fungal (35.4%) and bacterial (28.2%) colonies also exhibited noteworthy enhancements with the application of 100% RDF with 75 mL of Panchgavya over the control. The study concludes that the application of 100% RDF with 75 mL of Panchgavya increased quality cut flower production in alstroemeria.

This study investigated the potential risks of cadmium (Cd) toxicity in buffaloes grazing on forages that were cultivated in soils irrigated by various sources of wastewater. The absorption of trace metals (TM) by plants and their subsequent entry into the food chain pose a significant danger to grazing animals through the accumulation of contaminated fodder. The mean concentration of Cd in the water ranged from 0.18–1.78 mg/L, in the soil 0.63 to 1.87 mg/kg, in the forage 0.20 to 1.32 mg/kg, and in the blood 0.26 to 1.98 mg/L. Among all three sites, canal water (CW Site I), groundwater (GW Site II), and sewage water (SW Site III), the concentration factor (CF) values were below the threshold of 1 (CF < 1), indicating the nominal environmental concern regarding Cd contents in the soil-plant interface. In addition, a prominent variation was noticed in the transfer factor (TF) of Cd across different sites, with the highest TF observed in Avena sativa L. at SW Site III (0.8) and the lowest in Pennisetum glaucum L. at CW Site I (0.27). Furthermore, the hazard quotient (HQ) exhibited a substantial fluctuation, ranging from 0.39 to 2.6, reflecting varying levels of potential health risks associated with Cd exposure. The outcomes of the current investigation suggested that the prominent increase in Cd levels was recorded at sampling site SW Site III due to continuous wastewater irrigation. Prolonged exposure and increased Cd absorption in buffaloes grazing at these sites could have harmful long-term effects on their health. The correlation analysis between Cd concentrations in water, soil, forage, and blood showed a positive but non-significant relationship for water-soil, soil-forage, and forage-blood interactions. This highlights the need for further research to assess the long-term implications of wastewater irrigation on heavy metal accumulation in livestock.

Sufficient available water in the soil is critical for plant growth, especially soon after planting. Water absorbed by organic hydrosorbents has the potential to reduce drought stress after planting and improve seedling survival. This research evaluates the effectiveness of granular organic hydrosorbents (GOH) in increasing soil-water retention in a pot experiment with boxwood (Buxus sempervirens L.) seedlings. The leaf chlorophyll content was also evaluated to determine plant growth. The following fertilisers were used: GOH 1 comprised 50% biogas products and 50% wheat straw, and GOH 2 combined post-harvest grain, legume and oilseed residues. The fertilisers were applied to the bottom of the pot or mixed with the soil. Both types doubled the initial water retention compared to the control. While the control water retention dropped below zero after 18 days without watering, retention in the fertilised treatments ranged from 0.5 to 0.76 L. Re-watering part of the trial after the 18 days then induced increased retention to the initial levels in all treatments and the control. However, the fertilised plant’s water retention was up to half a litre greater than in the control when the 62-day experiment ended. The effect of GOH application was also manifested in the chlorophyll content. In the no watering mode, the chlorophyll content was, on average, 20% and 13% higher in the treatments with GOH 1 and GOH 2 than in the control. In the watering regime, the application of GOH 1 and GOH 2 increased the chlorophyll content by an average of 31% and 26%, respectively. Finally, these trials established that the GOH applications significantly delayed boxwood-seedling drying and increased their water retention and chlorophyll content in unwatered and re-watered plants.