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Results 541 to 570 of 4099:

Rheological properties of banana fruit in the creep test: Effect of variety and ripeness levelOriginal Paper

Nursigit Bintoro, Bambang Purwantana, Bayu Nugraha, Surya Abdul Muttalib, Slamet Sulistiadi, Vivien Fathuroya

Res. Agr. Eng., 2025, 71(3):163-173 | DOI: 10.17221/12/2025-RAE


Banana quality is influenced by many factors, including variety and level of fruit ripeness. This quality can be evaluated from various points of view, one of which is the rheological consideration. Rheological properties are very important to study because they determine the design of equipment and processes, and minimise product damage. The aim of this research was to analyse and model the effect of variety and ripeness level on the rheological properties of banana fruit by applying a creep test. This research was carried out using a factorial experimental design 3 × 3 with 5 replications. Three varieties of banana, namely Ambon (Musa parasidiaca var. sapientum L. Kunt.), Raja (Musa parasidiaca L.), and Kepok (Musa acuminata balbisiana Colla) and each in three levels of ripeness, namely mature green, half ripe, and ripe. It was found that the parameters of the rheological properties of bananas changed according to the ripeness level (P < 0.05). The values of these rheological parameters decreased as the bananas ripened. Meanwhile, the variety and the interaction between variety and ripeness had no significant influence on the rheological parameters measured (P > 0.05). The constants of the four-element Burger model changed with the ripeness in all banana varieties. The Burger model with four elements could accurately predict the strain value of bananas tested in the creep test.

Carcass traits and meat quality in Balkan goats: A multivariate evaluation of crossbreeding and slaughter weight effectsOriginal Paper

Nikola Stani¹iæ, Bogdan Cekiæ, Nevena Maksimoviæ, Maja Petrièeviæ, Nikola Deliæ, Slavi¹a Stajiæ, Tanja Ke¹kiæ

Czech J. Anim. Sci., 2025, 70(10):438-449 | DOI: 10.17221/53/2025-CJAS


This study investigated the effects of slaughter weight and breed on carcass characteristics, meat quality, and technological properties in purebred Balkan and F1 crossbred (Balkan × Saanen) male goats. A total of 48 animals, divided into four slaughter weight groups (10, 15, 18, and 22 kg), were analysed for carcass composition, muscle traits, and fat distribution. ANOVA and multivariate analyses (PCA and HCA) revealed significant effects of weight and breed on key carcass and meat quality parameters. Heavier animals exhibited higher intramuscular fat and primal cut yields with superior water-holding capacity (WHC), while lighter goats had leaner carcasses with less muscle pigment. Crossbred F1 goats demonstrated a more balanced muscle-to-fat ratio, whereas pure Balkan goats tended to accumulate fat more rapidly at higher weights. These findings indicate that optimal slaughter weight selection should align with market demands, such as producing leaner meat in lighter Balkan goats, or achieving increased marbling and better economic returns in heavier crossbred animals. Future research should explore genetic effects on muscle and fat deposition and assess consumer preferences to refine breeding and production strategies.

Experimental ultrasound stimulator for improving the diffusion of exosomes and drugs into lung tissueOriginal Paper

J Prucha, J Skopalik, T Parak, P Bratka, J Cuprova

Vet Med - Czech, 2025, 70(11):417-426 | DOI: 10.17221/41/2025-VETMED

Pulmonary fibrosis is not only a consequence of the recent COVID-19 pandemic, but is increasingly recognised by both human and veterinary healthcare providers. Pulmonary fibrosis is a progressive condition that leads to a decline in respiratory function and even death. In this work, we compared MSC-derived exosomes with conventional anti-inflammatory drug treatments. Exosomes from stimulated MSCs displayed higher miRNA concentrations (in particular, miRNA-30b was significantly increased). A set of rats with induced lung fibrosis were divided into four groups: NC (control – no treatment), A2 (exosome infusion), A3 (exosome infusion combined with ultrasound stimulation), and F1 (tamoxifen/metformin drug treatment). The rats’ lungs were subjected to histological analysis; the fibrosis scores for groups F1 and A3 were very similar and decreased significantly compared with group NC. Ultrasound-facilitated diffusion of exosomes from the capillaries into the lung tissue could represent an innovative therapeutic approach for slowing fibrosis and prolonging the active life of the organism.

Quantitative and qualitative changes in the green mass protein of white lupin during the growing seasonShort Communication

Eva Straková, Pavel Suchý

Plant Soil Environ., 2025, 71(11):820-828 | DOI: 10.17221/334/2025-PSE

The aim of the study was to assess the quantitative and qualitative changes in crude protein of the white lupin (Lupinus albus L.) green mass during the growing season in stands of three cultivars of white lupin (ZULIKA, AMIGA, DIETA), intended for feeding purposes as protein roughage, when grown under the same soil and climatic conditions in the Czech Republic. Changes in the crude protein and amino acid content were monitored during the growing season from the 9th to the 18th week of stand age. Changes in the crop dry weight were characterised by a statistically significant (P ≤ 0.05) decrease in crude protein from the 9th to the 15th week of stand age (ZULIKA 203.50–176.82 g/kg, AMIGA 190.58–161.59 g/kg, DIETA 201.41–175.84 g/kg). In the following period, during the maturation of lupin pods, from the 15th to the 18th week, the change in the crude protein content of the green matter was not statistically significant (ZULIKA 176.82–162.12 g/kg, AMIGA 161.59–150.95 g/kg, DIETA 175.84–175.24 g/kg). For most of the amino acids studied, a decrease in their content in the dry weight of the green matter was demonstrated from the 9th to the 15th week, with a subsequent statistically significant (P ≤ 0.05) increase from the 15th to the 18th week of stand age. Interesting differences were observed in the arginine content, which showed a statistically significant increase (P ≤ 0.05) during the growing season (ZULIKA 7.93–16.03 g/kg, AMIGA 6.88–13.04 g/kg, DIETA 7.56–17.45 g/kg). Changes in the dry weight of the crop in the crude protein and amino acid content can be considered characteristic of lupin crops because of the identical evidence in all three white lupin cultivars studied.

Effect of harvest time on total phenolic and flavonoid contents and antioxidant capacities of two grape varieties from Trebinje vineyard areaOriginal Paper

Mersija Deliæ, Milenko Blesiæ, Fikreta Behmen, Anstasija Anðu¹iæ, Fahrudin Topèiæ, Senad Murtiæ

Hort. Sci. (Prague), 2025, 52(4):304-312 | DOI: 10.17221/52/2025-HORTSCI

The aim of this study was to analyse the effects of harvest timing on the total phenolic and flavonoid contents, in addition to the total antioxidant capacity in the berries of ‘®ilavka’ and ‘Vranac’ varieties grown in the Trebinje vineyard area. Grape samples for both varieties were collected at three distinct points during their ripening. The chosen harvest dates were set 10 days apart, and the optimal date for grape harvesting was determined by assessing the sugar content and titratable acidity present in the grapes. Total phenolics, total flavonoids and total antioxidant activity of grape samples were determined by the Folin-Ciocalteu method, aluminium chloride method, and ferric reducing antioxidant power assay, respectively. The total phenolic and flavonoid contents, along with the total antioxidant capacity in the ‘Vranac’ grape, increased from the first to the second harvest date, but a decline was noted at the third harvest. ‘®ilavka’ grape exhibited an increase in total phenolic and flavonoid contents, as well as total antioxidant capacity from the first to the second harvest date, with no decrease recorded by the third harvest. As expected, the red grape variety ‘Vranac’ contained higher levels of phenolics and flavonoids than the light-skinned grape variety ‘®ilavka’.


Towards diverging land prices in agricultural districts? Evidence from Italy before and after the great crisisOriginal Paper

Emma Bruno, Rosalia Castellano, Gennaro Punzo, Luca Salvati

Agric. Econ. - Czech, 2023, 69(3):119-127 | DOI: 10.17221/395/2022-AGRICECON


In recent decades, farmland markets have risen sharply due to their attractiveness as safe investment and savings allocation instruments. This growth has occurred globally at different rates due to the uneven expected returns, and potential land uses in different regions. This paper provides evidence of the convergence of Italian farmland markets between 1990 and 2019 by using data on average land prices with a geographically detailed resolution that considers both administrative and altitude aspects of territories. The analysis combines traditional economic convergence approaches with spatial data exploration techniques. The results are consistent and show that territorial disparities in Italian farmland markets tend to increase over the study period. This proves that divergence patterns reflect the well-established North-South dualism and disparities in socio-economic characteristics.

Promotional effects on naturally occurring lactic acid bacteria without impairing chickpea germinationOriginal Paper

Eli¹ka Kováøíková, Veit Ny, Miloslav ©ulc, Jana Rysová, Natálie Peèenková, Milan Hou¹ka

Czech J. Food Sci., 2024, 42(2):85-92 | DOI: 10.17221/12/2024-CJFS


Sprouting has been used widely to enrich the nutritional quality of cereals and legumes. It improves the bioavailability of nutrients, especially those bound to phytic acid. However, sprouting is a good medium for microbial growth; thus, producing safe sprouts from harmful microbial growth is challenging. In food biotechnology, lactic acid bacteria (LAB) can be potentially used to improve nutrition and play a vital role as competitive microbes in food preservation. Therefore, supporting natural LAB growth by adding glucose sources during sprouting can produce a safer sprouting medium. Chickpeas (Cicer arietinum L.) sprouted for up to 50 h with glucose (0.1% and 1%) under aero-anaerobic conditions, with recycled water periodically spraying on the sprouts to support the natural LAB growth. Results show increased LAB counts, lactic acid and acetic acid, and decreased pH. Moreover, the addition of glucose had no significant detrimental effects on sprout quality compared to the control sample relative to nutritional compounds, such as saccharides, which remained similar. This sprouting method can be scaled up to production levels and is considerably cheaper than other treatments.

Assessment of deltamethrin-induced DNA damage, neurotoxic and neuroimmune effects in the brain tissue of brown trout (Salmo trutta fario)Original Paper

T Karatas, M Cakir

Vet Med - Czech, 2024, 69(3):77-83 | DOI: 10.17221/115/2023-VETMED


This study investigated the impact of deltamethrin (DM) toxicity on brown trout (Salmo trutta fario), examining its effects on the immune system, including the white blood cell (WBC), lymphocyte (Lym), total immunoglobulin (T. Ig), and lysozyme levels, as well as its neurotoxic consequences on the brain tissue. The neurotoxic effects encompassed oxidative stress, the activity of the antioxidant enzymes, such as the superoxide dismutase (SOD) and catalase (CAT), acetylcholinesterase (AChE) activity, and DNA damage using 8-hydroxy-2-deoxyguanosine (8-OHdG). The DM exposure led to elevated levels of malondialdehyde (MDA), and 8-OHdG, while concurrently causing a reduction in the AChE activity, protein and lipid content, WBC count, Lym, lysozyme activity, T. Ig levels, as well as the SOD and CAT levels in the brain tissues of groups 2 and 3 when compared to those in group 1. In summary, the findings of this study strongly indicate that DM induces DNA damage, immunotoxicity, and neurotoxicity in the brain tissue of brown trout, primarily due to the excessive production of reactive oxygen species (ROS). Moreover, the observed dose-dependent responses of DM to the environmental concentrations on all the investigated parameters suggest its potential utility in aquaculture risk assessment.

Modelling of desorption isotherms for dried meat: New approach and newly applied modelOriginal Paper

Pøemysl Richtr, Josef Bauer, Svatopluk Henke, Rudolf ©evèík

Czech J. Food Sci., 2024, 42(1):55-63 | DOI: 10.17221/170/2023-CJFS


In this paper, desorption isotherms of two jerky products were studied (whole-muscle jerky – sample 1 and minced jerky – sample 2). The work focused on the comparison of the Dynamic Dewpoint Isotherm (DDI) method and the Saturated Salt Slurry (SSS) method and testing the newly applied model for modelling desorption isotherms for dried meat. Data were statistically processed using 8 models [Guggenheim-Anderson-de Boer (GAB), Double Log Polynomial (DLP), Henderson, Chin, Smith, Oswin, Hasley, and newly applied model] and statistically evaluated using coefficient of determination (R2), root mean squared error (RMSE) and mean relative percentage deviation (P-value). The DLP model (25 °C) reached R2 ≥ 0.999, P-value ≤ 1.84 for DDI, and R2 ≥ 0.998, P-value ≤ 4.37 for SSS method. The GAB model reached R2 ≥ 0.997, P-value ≤ 2.58 for DDI, and for SSS method the GAB model reached R2 ≥ 0.998, P-value ≤ 5.47. The new model reached P-value ≤ 5.73 for DDI and P-value ≤ 3.48 for SSS method. All models reached the P-value < 10% except for Smith and Chin models. The DDI method and newly applied model prove to be a suitable and precise approach to the evaluation of isotherms of dried meat products.

Effect of seed weight and biostimulant seed treatment on establishment, growth and yield parameters of winter oilseed rapeOriginal Paper

David Beèka, Lucie Beèková, Matìj Satranský, Kateøina Pazderù

Plant Soil Environ., 2024, 70(5):296-304 | DOI: 10.17221/98/2024-PSE

This study evaluated the effect of seed weight and seed treatment on yield and growth parameters in a three-year field trial. Seed weight treatments were tested in three levels of thousand seed weight (TSW): low 4.28 g, medium 5.00 g and high 5.69 g, with and without a biostimulant seed treatment of Lumibio Kelta. Although statistically significant differences were not found in all experimental years, the three-year results showed that sowing seeds with higher seed weight increased the initial and final stand counts, root length, seed yield and oil content. The final stand at harvest time averaged over the three years ranged from 23.0 (low TSW) to 29.5 plants per 1 m2 (high TSW). Plants from high TSW seed had an average of 1.1 cm longer roots than plants from low TSW seed. The average yield increased with TSW: 5.49 t/ha (low TSW), 5.86 t/ha (medium TSW), and 5.94 t/ha (high TSW). High TSW also yielded higher oil content (45.77%) compared to the medium (45.25%) and low TSW (45.27%). No statistical difference could be detected in the initial emergence counts or final seed quality according to seed treatment. Seed treatment with the biostimulant increased root length and seed yield. Plants from seeds treated with the biostimulant had roots with an average of 0.6 cm longer, yielding 0.2 t/ha higher than the non-treated ones.

How does single- or double-cropped rice policy influence spatially irrigated land value in China?Original Paper

Yuqun Dong, Yaming Zhuang

Agric. Econ. - Czech, 2024, 70(6):279-290 | DOI: 10.17221/49/2024-AGRICECON


Rice cropped policy has been constantly changing, affecting the value of irrigated land. This study aims to examine distribution patterns of irrigated land value from a new perspective of uncertainties in single- and double-cropped rice policies in China. The real options approach was employed to calculate irrigated land value under policy uncertainty, and Moran’s index was applied to identify the spatial distribution characteristics of irrigated land value at the provincial level. The empirical results were as follows: significantly positive spatial correlations existed between the land value under two cropped rice policies. The northeast region of China had a low-value clustering effect based on geographical characteristics. In contrast, the southern provinces of China had a high-value clustering effect based on the criteria of administrative regions. This study considered policy uncertainty when evaluating the economic effect on irrigated land when revealing spatial correlation in land value. We provide a theoretical and empirical basis for the formulation of cropped rice policies.

Adjusting the sowing date of fresh maize to promote grain filling, key starch synthesis enzymes, and yieldOriginal Paper

Lin An, Hailong Wei, Yi Cheng, Jun Zou, Jin Zuo, Dailing Liu, Bi Song

Plant Soil Environ., 2024, 70(7):438-453 | DOI: 10.17221/490/2023-PSE

Clarifying the effects of meteorological factors on the growth and development of fresh maize after delayed sowing is important for selecting appropriate sowing dates and improving yield. Six sowing dates (B1 (March 10); B2 (March 20); B3 (March 30); B4 (April 9); B5 (April 19), and B6 (April 29)) and three fresh maize cultivars (A1 (Wan Nuo 2000); A2 (Nongke Nuo 336), and A3 (Caitian Nuo 6)) were chosen for experiments conducted between 2021 and 2022 in Guiyang, Qingzhen City, China. The results showed that the whole growth period and sowing-silking period were significantly reduced with delayed sowing, while the grain-filling period was relatively stable. Delayed sowing was beneficial in increasing the number of endosperm cells and the weight of the hundred kernels. The graining filling rate and the activities of four key starch synthesis enzymes (sucrose synthase, ADP-glucose pyrophosphorylase, starch branching enzyme, and starch debranching enzyme) were significantly influenced by light, temperature, and precipitation, and they mainly affected the hundred kernel weight. The yield tended to increase with delayed sowing, and the correlation analysis between precipitation and yield at different sowing periods showed a significant effect of precipitation on yield. Delaying the sowing to mid-early April was more favourable for grain filling, enhanced key enzyme activity, and increased the kernel weight and yield. These results highlight the importance of choosing excellent cultivars and matching them with the most suitable sowing date to fully exploit climatic resources and achieve high-yield and high-efficiency cultivation of fresh maize.

Phytochemical, antioxidant activity, and thermal stability of Clitoria ternatea flower extractsOriginal Paper

Sy-Yu Shiau, Yuhan Wang, Yanli Yu, Songling Cai, Qian Liu

Czech J. Food Sci., 2024, 42(4):284-294 | DOI: 10.17221/68/2024-CJFS


The demand for beneficial, safe natural pigments is growing. Clitoria ternatea (CT) flowers are a promising source of natural blue colours and bioactive compounds. This study extracted intact CT flowers (CTFs) and their powders (CTFPs) with distilled water at various temperatures (30–90 °C) and durations (30–180 min). The total anthocyanin content (TAC) and total phenolic content (TPC) of the CT extracts were analysed by using the pH differential and Folin-Ciocalteu methods, respectively. The antioxidant activity was expressed as the ability to scavenge DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The results showed that different sample types significantly (P < 0.05) influenced the TAC, TPC, and antioxidant activity. The optimal TAC and TPC extraction for CTF was at 90 °C for 90 min, and for CTFP at 90 °C for 30 min. Using hot water instead of 60% alcohol organic solvent was feasible for extracting the phytochemicals, particularly with CTF. Ultrasound-assisted extraction was notably effective for anthocyanins from intact flowers. Higher heating temperatures and pH values increased the anthocyanin degradation rate constants, while a higher pH decreased the activation energy. Thus, blue aqueous CT flower extracts, rich in phytochemicals and antioxidants, have the potential for use in foods and nutraceuticals with neutral and acidic pH values.

Effect of acetic acid immersion on the taste and aroma quality of immature Robusta coffee beansOriginal Paper

Deden Fardenan, Dian Angraini Suroto, Supriyadi Supriyadi

Czech J. Food Sci., 2024, 42(6):391-404 | DOI: 10.17221/60/2024-CJFS


The presence of immature coffee beans reduces the quality of the coffee brew. This study evaluated how the acetic acid affected the aroma and taste of immature compared to mature Robusta coffee. The naturally processed immature Robusta green beans were immersed in acetic acid (0–5%) for 30–90 min. The naturally processed mature Robusta green beans were used as a control treatment. The samples were roasted at a medium level (240 °C, 14 min). The sensory analysis was evaluated by the cupping test by a trained and certified panellist (Q-Grader). Acetic acid immersion significantly improved the sensory quality of immature beans (P < 0.05). Specifically, immersion in 3% acetic acid for 90 min yielded the highest sensory score of 84.92. According to the Coffee Quality Institute (CQI) classification, this score falls into the 'Fine' Robusta classification. The 0% 30-min, 3% 30-min, and 3% 90-min treated immature samples and an untreated control mature sample were analysed for pH, caffeine, chlorogenic acid, and volatile compounds. The treatment significantly decreased the chlorogenic acid (P < 0.05) and increased the volatile compound concentration of furans, ketone, pyrrole, aldehyde, and ester while decreasing pyrazines, sulphur, phenol, and alcohol. This study effectively enhanced immature Robusta taste and aroma quality to a degree comparable with mature Robusta coffee beans.

Claw diseases are the dominant cause of lameness in dairy cows and a risk factor for mastitisOriginal Paper

V Hisira, J Zahumenska, M Kadasi, R Klein, P Mudron, F Zigo

Vet Med - Czech, 2025, 70(2):35-44 | DOI: 10.17221/56/2024-VETMED

Lameness and mastitis are two of the most economically important issues for the dairy industry. This study aimed to obtain a clearer analysis of the link between mastitis and lameness in dairy cows using the Mastitis Score and detecting the pathogens that predominate in dairy cows’ milk samples with claw diseases. During routine claw trimming, milk samples were collected from dairy cows in two Slovak dairy farms. Out of 558 cows presented for claw trimming, 144 cows (25.8%) exhibited lameness from claw diseases. The most prevalent disease in our study was digital dermatitis (43.1%), followed by toe necrosis (41.7%), and Rusterholz ulcer (15.3%). Udder inflammation in clinical form was diagnosed based on clinical examination of individual udder quarters, and sensory evaluation of milk from each quarter and in the subclinical form by the CMT. Mastitis was detected in 80 cows with claw diseases, clinical mastitis in 14 cows (17.5%), and subclinical mastitis in 66 cows (82.5%). On both farms, Mastitis Scores were significantly higher in dairy cows affected by claw diseases than in the non-lame ones. In mastitic cows affected by claw diseases, environmental pathogens dominated the infected milk samples. Our findings showed that cows with claw disease were more likely to have mastitis.

Analysis of the mare breeding population of Haflinger breed in the Czech RepublicOriginal Paper

Michaela Kuli¹»áková, Eva Sobotková, Radek Filipèík

Czech J. Anim. Sci., 2025, 70(3):83-92 | DOI: 10.17221/8/2025-CJAS

The aim of this study was to analyse the conformation traits and body dimensions of Haflinger mares registered in the Czech Republic studbook. The study focused on the effects of age at studbook entry, year of birth, proportion of Arabian genes, and country of origin on various parameters. Data were collected from 825 mares, including scores obtained during the studbook entry evaluation and body measurements such as withers height measured with a stick (WHS), withers height measured with a tape (WHT), chest girth (CG), and cannon bone circumference (CBC). Based on these data, the bone index (BI) and body massiveness index (MI) were calculated. Statistical analysis was performed using multifactor analysis of variance (ANOVA) and Scheffe’s post-hoc testing method. The results showed that the age at studbook entry significantly affected conformation scores, chest girth, cannon bone circumference, and both indices (MI, BI). Mares aged 3–4 years had significantly higher conformation scores compared to mares aged 5–6 years (P = 0.001) and mares aged 7 years and older (P = 0.000). Mares aged 5–6 years and those aged 7 years and older showed higher CG values (P = 0.002, P = 0.000) and CBC values (P = 0.010, P = 0.001). Furthermore, the year of birth had a highly significant impact on all variables studied. The withers height of mares born between 2011 and 2020 was, on average, 3.49 cm higher (144.84 cm) than that of mares born between 1987 and 2000 (141.35 cm) (P < 0.000). Regarding CG, a statistically significant increase was observed in mares born in 1987–2000 and 2001–2010, with chest girth increasing from 180.45 cm to 183.76 cm (P = 0.016). However, a subsequent decrease to 179.91 cm was noted in mares born between 2011 and 2020 (P = 0.002). The influence of the proportion of Arabian genes was significant for WHS, where mares with a higher proportion of Arabian genes (>1.56%) showed lower WHS (141.74 cm) compared to mares with a lower proportion (143.86 cm) or no Arabian genes (143.01 cm). While ANOVA demonstrated a significant effect of Arabian genes on CBC, Scheffe’s post-hoc test did not confirm this finding. No statistically significant differences were observed in other body dimensions based on the proportion of Arabian genes. The influence of the country of origin was statistically significant for all variables except WHS and WHT. Regarding the conformation scores, mares from the Czech Republic scored lower (6.98) than Austrian mares (7.22, P = 0.000). Additionally, CG was significantly lower in Austrian mares (178.58 cm) compared to Czech mares (182.54 cm, P = 0.000). Austrian mares also had significantly lower MI (P = 0.000) and BI (P = 0.040), with Austrian indices at 124.71/13.31 and Czech indices at 127.77/13.41. Based on this empirical evaluation of the current and original population of Haflingers in the Czech Republic, the connections and influence of individual factors during breeding are revealed. The results of our analysis have built an objective scientific basis and they allow the breeding organisation to determine the further breeding process.

Determination of the variation in volatile components during the ripening of Sorbus domestica L. fruitOriginal Paper

Engin Gür, Mehmet Ali Gündoğdu, Tuba Baºaran

Hort. Sci. (Prague), 2025, 52(1):53-60 | DOI: 10.17221/157/2023-HORTSCI

Sorbus domestica L. is a deciduous shrub-like tree in the Rosaceae family that reaches different heights (3–25 m). The service tree commonly grows in the Marmara region, Central and Western Black Sea regions, and Central Anatolia in Türkiye. The fruit of the service tree called Sorbus domestica, has an essential place in regards to human health in terms of its antioxidant values. The aroma components and phenolic compounds it contains display a variety of physiological traits. This study was carried out to determine the changes in the pomological characteristics and volatile compounds of Sorbus domestica L. fruits in the Tokat region at harvest and the consuming maturities in 2019 and 2020. The aroma extraction from the Sorbus fruits identified esters, alcohols, ketones, aldehydes, terpenes, and other volatile aroma compounds. As a result of the study, differences in aldehydes, esters, and terpene compounds were notable within the scope of the volatile aroma compounds. According to the obtained information, our results identified total aldehydes of 70.64%, esters of 7.49%, and terpenes of 21.35% in Sorbus domestica during the harvest maturity. When Sorbus fruits were left at room temperature until consuming for consumption was reached, the volatile compound rates varied which were 60.59% for the aldehydes, 22.49% for the esters, and 13.20% for the terpenes.

Pathogenic bacteria in biogas plants using cattle, swine, and poultry manureOriginal Paper

L Cermak, E Pechouckova, M Marounek, T Paulova

Vet Med - Czech, 2025, 70(5):151-155 | DOI: 10.17221/47/2024-VETMED

Fugate, a waste product from biogas production, regularly used in agriculture as a fertiliser, may contain bacterial pathogens that cause zoonoses. Anaerobic digestion (AD) can inactivate viable pathogens, including parasites, viruses, and pathogens containing antibiotic resistance genes. This study aimed to compare the numbers of pathogenic bacteria and diversity of potential bacterial pathogens in the fugate using three different types of slurry: cattle, swine, and poultry manure. The swine fugate showed higher numbers of Clostridium perfringens and Campylobacter sp. than the poultry and cattle fugate. In the cattle fugate, the lowest total number of pathogenic bacteria and a low number of coliforms were detected after the AD. The use of cattle manure in biogas plants presents a lower potential for soil contamination with pathogens. The fugate produced using poultry or swine manure can be used carefully to avoid possibility of contamination of aquifers or surface waters. Also fugate produced from manure of cows suffering from chronic botulism  can be used only with carefulness because of the presence of Clostridium botulinum spores in biogas waste of diseased cows.

Effects of soaking seeds with selenite on the physiological characteristics and quality of peanut sproutsOriginal Paper

Sanchun Lei, Qiong Wu, Yuehong Liu, Minghao Hao, Rui Liu, Feiyan Yu, Lianhe Zhang

Plant Soil Environ., 2025, 71(6):387-397 | DOI: 10.17221/160/2025-PSE

The aim of this study was to investigate the physiological characteristics and quality of hypocotyls in the production of selenium-enriched sprouts from peanut seeds soaked in selenium (Se) solution. Peanut seeds were soaked with 0, 2.5, 5.0, 7.5, and 10 μmol/L Na2SeO3 for 12 h and then germinated. The results showed that the selenium concentration in peanut shoots increased with increasing levels of selenite soaking, and there existed a crossroads of selenite soaking concentration (5.0 μmol/L) when selenium concentrations in cotyledons and hypocotyls were equal. Below and above this concentration, Se concentrations in shoots were radicle > cotyledon > hypocotyl or cotyledon > radicle > hypocotyl, respectively. In addition, Se significantly promoted the elongation of hypocotyls and radicles, increased shoot biomass, increased the activity of antioxidant enzymes and the concentration of antioxidants in hypocotyls, and decreased malondialdehyde levels. Moreover, Se significantly increased the concentrations of soluble sugars, proteins, free amino acids and resveratrol in hypocotyls. These results indicate that soaking peanut seeds with selenite significantly increased Se concentration, biomass, antioxidant capacity and quality of peanut shoots. This study provides a theoretical basis for the rapid and standardised production of Se-enriched peanut shoots from selenite-soaked seeds.

Chickpeas (Cicer arietinum L.) and oats (Avena sativa L.) pre-gelatinised flour for instant food productsOriginal Paper

Bruna Mayara Roldão Ferreira, Isadora Maria Melo Torres, Gabriel Sarache, Jean Lopes Silva, Claudia Cirineo Ferreira Monteiro, Benício Alves Abreu Filho, Carlos Eduardo Barão, Tatiana Colombo Pimentel (ORCID: 0000-0003-4

Czech J. Food Sci., 2025, 43(4):246-254 | DOI: 10.17221/215/2024-CJFS

Chickpeas and oats are rich in essential nutrients and bioactive compounds, such as phenolics and flavonoids. Extrusion technology enhances food digestibility, nutrition, and shelf life, thus meeting consumer demands. Instant food products are experiencing market growth due to advancements in processing technologies that cater to healthier ingredients. This study aims to evaluate pre-gelatinised flours produced through extrusion using different proportions of chickpeas and oats (100 : 0, 90 : 10, and 80 : 20) and compare them with their respective raw versions. The physicochemical properties, technological characteristics [Rapid Viscosity Analysis (RVA) and Water Absorption Index (WAI)], applications within the instant food industry, and their potential for acceptance were evaluated. The extruded flours showed lower moisture content and water activity – finally, their application in instant soups, mainly at 10%, increased consumer acceptance. Incorporating oats in the flours resulted in higher water activity, WAI, final viscosity, peak viscosity and pasting temperature. Our results demonstrate that flours with suitable physicochemical and technological properties could be obtained using chickpeas, oats, and extrusion. Its incorporation into instant soups resulted in products with suitable acceptance by consumers.

Physiological and biochemical bases of AMF-mediated antimony stress tolerance in Linum usitatissimum: enhancing growth, phytochemical production, and oxidative damage resilienceOriginal Paper

Ahlem Zrig, Shereen M. Korany, Hana Sonbol, Emad A. Alsherif, Foued Hammouda, Danyah A. Aldailami, Marwa Yousry A. Mohamed, Mohamed S. Sheteiwy, Maria Gabriela Maridueña-Zavala, Salma Yousif Sidahmed Elsheikh

Plant Soil Environ., 2025, 71(9):650-665 | DOI: 10.17221/246/2025-PSE

Antimony (Sb) pollution from industrial activities poses a severe global threat, particularly impacting valuable medicinal crops like linseed, which are highly sensitive to heavy metals. This study reveals the remarkable potential of arbuscular mycorrhizal fungi (AMF) as a sustainable solution to this challenge. Our research demonstrates that while Sb stress significantly impairs linseed growth and photosynthesis, it also triggers oxidative damage. AMF improved photosynthetic performance and water status, and notably enhanced the biosynthesis of crucial phytochemicals like phenolics, flavonoids, and citric acid. These compounds are vital for both plant defence and human health. Furthermore, AMF promoted the accumulation of essential detoxifying agents, leading to a better redox balance and significantly reducing Sb uptake and translocation by 47%. This dual action not only bolsters the plant’s tolerance to Sb but also enhances its medicinal value by boosting health-promoting bioactive metabolites. These promising findings underscore AMF’s dual role: a powerful tool for phytoremediation and a natural enhancer of phytochemical quality. Arbuscular mycorrhizal fungi provide a sustainable, nature-inspired approach to safely cultivate medicinal plants in environments contaminated with heavy metals, underscoring the vital role of plant-microbe interactions in alleviating environmental stresses.

Far lateral lumbar intervertebral disc extrusion in a cat: Case reportCase Report

Y Jang, HK Chae, YJ Hong

Vet Med - Czech, 2025, 70(9):335-338 | DOI: 10.17221/107/2024-VETMED

A 4-year-old domestic long-haired cat presented with acute, progressive, non-weight-bearing lameness of the left pelvic limb and reluctance to climb. Magnetic resonance imaging (MRI) revealed decreased T2-weighted signal intensity of the nucleus pulposus in all lumbar intervertebral discs except L7-S1, with far-lateral T2-weighted low-signal material at L6–L7 surrounding the sixth lumbar nerve root. A diagnosis of far-lateral intervertebral disc extrusion at L6–L7 was made. Clinical signs resolved almost completely within 15 days of conservative management. To our knowledge, this is the first report describing MRI findings of far-lateral intervertebral disc extrusion in a cat, highlighting the importance of considering this condition in the differential diagnosis of acute unilateral pelvic limb lameness.

Rural e-commerce and agricultural total factor productivity: Evidence from ChinaOriginal Paper

Jialong Zheng, Xintong Liang, Junkai Ma, Guangsi Li

Agric. Econ. - Czech, 2025, 71(10):550-563 | DOI: 10.17221/289/2024-AGRICECON

Employing the Comprehensive Demonstration of E-commerce in Rural Areas (CDERA) policy as a quasi-natural experiment, this paper explores the impact of rural e-commerce development on agricultural total factor productivity (ATFP) and its underlying mechanisms, using a difference-in-differences model. The analysis utilises panel data from 1 495 counties in China from 2001 to 2021. The findings indicate that the CDERA policy implementation enhanced ATFP in treated counties by approximately 1.6 percentage points compared to control counties, other factors being equal. Mechanism analysis further reveals that the CDERA policy enhances ATFP by improving agricultural technological efficiency and fostering agricultural industrial development. This study highlights the significant role of CDERA policy in enhancing agricultural productivity in China and offers policy insights for advancing rural e-commerce and promoting sustainable agricultural development.

Decision tree analysis of the prospects of organic food: Evidence from China and HungaryOriginal Paper

Yue Wu, Andrea Tick, Katalin Takács-György

Czech J. Food Sci., 2025, 43(6):450-461 | DOI: 10.17221/205/2024-CJFS

With the growing population, increasing income, and high-speed lifestyle, people pay more attention to a healthy diet and nutritional food. Organic food, also regarded as healthy, sustainable, or eco-friendly eating, has gained global popularity. This trend has been particularly amplified by the COVID-19 pandemic, leading to a surge in demand for nutritious foods worldwide. Organic food originated in Europe, with the highest development globally. Its progress varies by country. China's organic food industry began decades ago, growing rapidly. As globalisation advances and China's economy accelerates, it is valuable to examine the organic food industries in Hungary and China. In order to understand the willingness of consumers to buy organic food, we conducted a survey for a month in 2021 from 581 respondents in Hungary (185), China (374), and other countries, compared the respondents' demographic data, organic food consumption and their buying willingness and attitude to recommend organic food to other consumers. The decision tree analysis was deployed to analyse the statistical data via SPSS software. It showed that organic food has a positive demand in both Hungary and China, but some purchase habits are different in these two countries.

The new EU Timber Regulation 2023/1115 and its legal implications for the Czech forestry and timber sectorReview

Petra Hlaváèková, Martin Cempírek, David Bøezina

J. For. Sci., 2025, 71(12):575-588 | DOI: 10.17221/82/2025-JFS

Regulation (EU) 2023/1115 on deforestation-free products (EUDR) establishes a substantially revised legal framework that extends beyond the previous EU Timber Regulation (EUTR 995/2010) by integrating legality, sustainability, and traceability obligations. This article provides a legal and comparative assessment of the implications of the EUDR for the Czech forestry and timber sector. The methodological approach combines doctrinal interpretation of EU legislation, comparative analysis of national implementation models, and an examination of relevant case law. Experiences from selected EU Member States are used to illustrate institutional, technological, and administrative challenges linked to EUDR implementation. The results indicate that, although the Czech Republic has an established regulatory framework under Act No. 251/2025 Coll., effective compliance with the EUDR will require improved coordination among supervisory authorities and the development of a unified digital platform for submitting and verifying due diligence declarations. Evidence from other European countries shows that integrated monitoring systems and the use of third-party certification can enhance both efficiency and transparency. The study concludes that the successful implementation of the EUDR in the Czech context will depend on institutional capacity, digital readiness, and the ability of forest managers and traders to adapt to more stringent environmental and traceability requirements.

Combined effect of nitrogen and phosphorous fertiliser on nitrogen absorption and utilisation in riceOriginal Paper

Yating Zheng, Hong Chen, Guotao Yang, Rudan Wang, Nabi Farhan, Chong Li, Cheng Liang, Kaiqin Shen, Xuechun Wang, Yungao Hu

Plant Soil Environ., 2023, 69(1):25-37 | DOI: 10.17221/420/2022-PSE

The objectives of this study were to investigate the nitrogen (N) and phosphorus (P) balance fertilization strategy in paddy fields, and to evaluate the effects on N uptake and utilization in rice. In 2017-2018, the experiment was conducted using Deyou4727 hybrid rice with four different P fertilizer levels (0, 30, 60, and 90 kg/ha), marked as P0, P1, P2, P3 in turn, and four different N levels (0, 90, 150, and 270 kg/ha), similarly marked as N0, N1, N2, N3 in turn. The results showed that in the N-insufficient (N0, N1) environments, the P1 treatment increased N uptake and promoted transfer to the grain. However, high-P (P3) application increased the dry matter accumulation than other P levels, but limited the production and translocation of dry matter to some extent. In N-sufficient (N2, N3) environments, P2 level increased crop yield and N use efficiency by 11.35% and 37.01%. Unlike P2, none-P (P0) and high-P levels decreased rice dry matter translocation and transport capacity, which further affected N uptake and utilization in N-sufficient environments. Overall, the combination of the N application rate of 90 kg/ha and P application rate of 30 kg/ha, N application rate of 150, 270 kg/ha, and P application rate of 60 kg/ha had a high yield; strong nutrient accumulation and transfer ability. It was more inclined to balance N and P, which was beneficial to plant N absorption and utilization.

Application of the physical properties of local cowpea varieties in the development of a multi-variety cowpea cleanerOriginal Paper

Babatunde Olusola Adetifa, Elizabeth Ayobami Olumomi, Taiwo Moses Samuel, Ayoola Abiola Babalola

Res. Agr. Eng., 2024, 70(2):82-91 | DOI: 10.17221/51/2023-RAE

Cowpea, an annual legume widely grown and consumed in Nigeria, has been observed to contain between 27–33% impurities when freshly harvested and threshed. This poses a threat to humans when consumed and in large-scale agricultural processing. Therefore, this study is aimed at developing and evaluating the performance of a multi-variety cowpea cleaner (MVCC). Using standard methods, some selected engineering properties of the cowpea varieties were examined and used in the design of the MVCC. The cowpea had a moisture content of 8–14%, depending on the locations and varieties. Other properties investigated include the length, width, thickness, sphericity, geometric mean diameter, unit volume, arithmetic mean diameter, aspect ratio, surface area, unit weight, true density, terminal velocity, and angle of repose. The MVCC comprised the hopper, winnower, cleaning unit, fan assembly, and frame. The performance of the MVCC was also evaluated. The efficiency of separating good products of honey, drum, and Sokoto White beans was 95, 91, and 84%, respectively, while separating bad products was 87, 94, and 96%, respectively.


Comparative study on the geographical indication protection between China and the European Union – From the perspective of the China-EU Geographical Indications AgreementOriginal Paper

Weiwen Qian, Yinguo Dong

Agric. Econ. - Czech, 2023, 69(5):185-201 | DOI: 10.17221/98/2023-AGRICECON

Geographical indication (GI) represents the specific good quality and reputation of the regional characteristics of agricultural products, which is a positive approach for stabilising the export of agricultural products, realising high quality and high prices for agricultural products and rural revitalisation. Based on the China-EU Geographical Indications Agreement, this paper discusses the differences between China and the European Union (EU) in terms of GI protection from three aspects: the institutional framework, the operational system, and the operational status of GIs. The purpose of the study is to identify the differences between China and the EU in the protection of GIs, China's shortcomings, and gaps, and to propose policy recommendations for China to protect GI products better, exploit the economic impact of GIs and enhance the competitiveness of China's exports.

Administrative system of afforestation in the Czech Republic: A long journey to a new forestReview

Martin Balá¹, Josef Gallo, Marcin Czacharowski, Michal Pástor, Jaroslav Jankoviè, Igor ©tefanèík, Ivan Kune¹, Hubert Hasenauer

J. For. Sci., 2024, 70(2):41-63 | DOI: 10.17221/75/2023-JFS

This review uses a case study based on an executed afforestation project to comprehensively describe the administrative procedure regarding afforestation of agricultural land in the Czech Republic, Central Europe. We consider the official proceedings for obtaining a permit to land-use change (i.e. changing the nature of land use and the mode of land use in the real estate cadastre) as a very complex and protracted procedure, which can substantially reduce the motivation of people to establish new forests. Therefore, we propose a simplification of the administration process of land-use change. The main idea is to make the procedure as simple as possible in those areas where afforestation is desirable, especially from the environmental point of view. It assumes the possibility of quick, precise and, if possible, automatic distinction whether the afforestation is suitable or not. The situation in the Czech Republic is compared with the surrounding Central-European countries: Poland and Slovakia. In these countries, the official procedure connected with afforestation seems to be rather less complicated.

The effects of biochar grain size on radish plants under low water availabilityOriginal Paper

Lenka Botyanszká, Justína Vitková, Natália Botková, Lucia Toková, Ján Gadu¹

Plant Soil Environ., 2024, 70(4):203-209 | DOI: 10.17221/414/2023-PSE

Low water availability is a significant constraint on global crop production. Exploration is needed regarding plant responses to drought in interaction with biochar, encompassing optimised water use and carbon allocation strategies. The size of the biochar particles also plays an important role, especially in influencing the dynamics of water and plant growth. This study explored the potential impact of biochar treatment on radish growth and drought tolerance. Finer biochar particles lead to the most substantial available water content for plants, increasing at around 30%, while medium and larger fractions increase by about 22% and 16%, respectively, compared to control soil. The chlorophyll fluorescence technique showed improved water management of drought stress at larger fractions of biochar. Our research underscores the potential of biochar treatments for environmental stresses and water scarcity in modern agriculture.

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