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Results 601 to 630 of 4099:

Probiotic lactic acid bacteria in biotechnology and the food industry: A reviewReview

Damla Avci, Simona Gillarová, Svatopluk Henke, Zdeněk Bubník, Marcela Sluková

Czech J. Food Sci., 2025, 43(2):75-89 | DOI: 10.17221/17/2025-CJFS

This review explores the diverse applications and health benefits of probiotic lactic acid bacteria (LAB) through biotechnological applications in the food industry. While all LAB are indispensable for the production of fermented foods thanks to their ability to produce lactic acid and bacteriocins that act as natural preservatives, specific strains of probiotic LAB offer targeted health benefits. In addition to general benefits of LAB, probiotic strains significantly enhance gut microbiota, enhance human immunity, and exhibit antimicrobial properties. This review also delves into the mechanisms of action of probiotic LAB, focusing on adhesion, colonisation, and antioxidant production, emphasising their potential to advance nutritional innovations. Beyond food production, the broader category of LAB has transformative potential in industrial applications, particularly in the sugar industry, where their metabolic activity can improve sucrose extraction processes, promote microbial management, and reduce unwanted by-products. By understanding these aspects, the review underscores the importance of probiotic LAB in promoting health, efficiency, and sustainability across sectors.

Antibacterial activity and compositional analysis of Acer truncatum bunge leaf extract against Staphylococcus aureusOriginal Paper

F Liao, J He

Vet Med - Czech, 2025, 70(5):166-176 | DOI: 10.17221/65/2024-VETMED


Staphylococcus aureus is a zoonotic pathogen that can cause various diseases in both humans and animals. This experiment evaluates the antibacterial activity of extracts from Acer truncatum leaves against S. aureus, including the minimum inhibitory concentration (MIC), growth curve, and cell membrane integrity assessment, alongside the identification of the extract components by LC-MS. Results demonstrated that the MIC of the n-butanol extract from the ethanol extract of Acer truncatum leaves against S. aureus was 3.125 mg/ml, with a minimum bactericidal concentration (MBC) of 6.25 mg/ml. Over 16 h, the extracts at concentrations of 0.25 MIC, 0.5 MIC, and 1 MIC effectively inhibited S. aureus growth. The fluorescence staining revealed that the extracts at different concentrations compromised the structural integrity of the cell membrane. The LC-MS analysis identified the principal constituents of the extract as betaine (27.189%), 3,4-dihydroxymandelic acid (16.112%), quercitrin (14.768%), chlorogenic acid (8.778%) and neochlorogenic acid (4.452%). The study indicated that Acer truncatum leaf extract has good potential for application in natural antibacterial drugs.

The effect of soya curd substitution for milk on physical and sensory properties of vanilla gelato productOriginal Paper

Hega Bintang Pratama Putra, Anang Muhammad Legowo, Ahmad Ni'matullah Al-Baarri, Siti Susanti

Czech J. Food Sci., 2025, 43(3):187-193 | DOI: 10.17221/209/2024-CJFS

The investigation was aimed to evaluate the physical (total solids, melting rate, viscosity, and overrun) and sensory properties of vanilla gelato (VG) made by substituting soya curd (SC) for milk. Samples were VG without SC (F0) and with SC, i.e. 25% (F1), 50% (F2), and 75% (F3). Each sample criterion consisted of 5 repetitions (n = 20). Results showed that F3 possessed the lowest total solids, viscosity, and overrun while its melting rate was the highest among other SC substitution levels (P < 0.05). The sensory test delineated that F3 had a less soft texture, although its aroma and flavour were not different from others (P < 0.05). Thus, the greater portion of SC substitution for milk in the VGmaking process would substantially affect the physical properties of its final product in which the total solids, viscosity, and overrun were lower while the melting rate was higher. Substituting SC for milk in VG did not affect the sensory properties, except the texture. The higher SC amount used for milk substitution would result in VG with a less soft texture. SC at a certain level of substitution was potentially expected as an innovation of the phyto-gelato product, namely soy gelato.

MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensisOriginal Paper

Praphat Kawicha, Ladawan Rattanapolsan, Ratri Boonruangrod, Yube Yamaguchi, Kusavadee Sangdee, Aphidech Sangdee, Thanwanit Thanyasiriwat

Plant Protect. Sci., 2025, 61(3):262-277 | DOI: 10.17221/179/2024-PPS

Peptides play regulatory roles in various plant development and defence processes. They function as molecular messengers that detect threats and trigger defence responses. This study aimed to identify the genes encoding endogenous plant elicitor peptide precursors (PROPEPs) in bananas and their role in inducing resistance to Ralstonia syzygii subsp. celebesensis (Rsc). Two precursor genes, MaPROPEP1 and MbPROPEP1, were discovered and predicted to encode the precursor proteins of elicitor peptides, namely, MaPep1 and MbPep1. Both elicitor peptides contained 23 amino acids of the active elicitor peptide, which activated innate immune responses in banana resistance to Rsc. The disease assessment was conducted by inoculating banana plants with Rsc isolate MY4101 using the root-stabbing method. The results demonstrated that MaPep1 and MbPep1 pretreatment enhanced resistance to banana blood disease, as indicated by reduced disease severity and the absence of wilting for 7 days after infection. The expression of the MaPROPEP1, MbPROPEP1, MaLOX7, and Pr-10 genes was evaluated using qPCR and found to be upregulated by MaPep1 and MbPep1 injection followed by Rsc infection in aboveground banana tissues within 7 days. These findings prove that MaPep1 and MbPep1 are members of the Pep family and exhibit conserved functions across various plant species. This approach may be used to develop strategies for enhancing disease resistance in banana cultivation.

Understanding meat and fish consumption: Socio-demographic and value insights from five European countriesOriginal Paper

Diana Kmeťková, Milan Ščasný, Iva Zvěřinová, Vojtěch Máca

Agric. Econ. - Czech, 2025, 71(7):394-409 | DOI: 10.17221/348/2024-AGRICECON

Current dietary patterns in developed countries, characterised by high intakes of processed and animal-source foods, are linked to increased obesity and diet-related non-communicable diseases, as well as environmental burdens. This paper investigates determinants of red meat, white meat, and fish consumption across five European countries, using representative survey data from over 10 000 individuals. Our findings reveal that men consume more red meat and fish than women, though, when adjusted for body weight, women consume significantly more white meat and fish. While vegetarians are mostly people younger than 35 years, meat eaters in the same age category tend to eat more red meat than older people. Cross-country differences highlight the need for localised policy approaches. Individual values also shape dietary choices. Security-oriented people prefer red meat, while altruistic individuals consume less of it. Biospheric values, while strongly associated with being vegetarian, show no significant association with meat or fish intake. To reduce red meat consumption, policies should highlight health benefits of eating less meat, with messages tailored to specific demographic groups. Additionally, enhancing meat alternatives' affordability, taste, and appearance is essential for promoting dietary shifts.

Rainfall induced changes in soil moisture: A comparative study of conventional and strip tillageOriginal Paper

Vojtěch Štros, David Kincl, David Kabelka, Jan Vopravil

Soil & Water Res., 2025, 20(4):234-242 | DOI: 10.17221/56/2025-SWR

Strip tillage is a very popular form of conservation tillage that is used in places with a higher risk of soil erosion. It is commonly accepted that strip tillage reduces the effects of water erosion; however, the exact way this effect is produced is very hard to quantify. This study focuses on the way strip tillage influences soil moisture and the way it changes with different intensities of rainfall, in comparison with conventional tillage. This study was conducted near Petrovice, Středočeský kraj, Czechia, over the course of four years (2021–2024). The conditions of all four test sites were comparable, both in terms of slope and soil type present. The soil moisture of strip tillage in a depth of 15 cm was changing differently in comparison with conventional tillage. During lower intensity rainfall events, the soil moisture of the strip tilled plot changed significantly less in comparison with conventional tillage. On the contrary, when more intense precipitation occurred, the soil moisture in the strip-tilled plot responded with significantly higher changes in comparison with conventional tillage. Soil drying after precipitation was also studied, with the speed of drying of strip tillage being higher than that of conventional tillage. These findings help better understand the changes strip tillage introduces into the soil and to the crops it is used with.

Effects of nutrient solutions on growth, yield and quality of yardlong bean plant (Vigna unguiculata subsp. sesquipedalis L.) grown in a hydroponic systemOriginal Paper

Thi Thuy Le, Phuong Thao Nguyen, Thi Van Pham, Thi Oanh Nguyen

Hort. Sci. (Prague), 2025, 52(4):333-341 | DOI: 10.17221/1/2025-HORTSCI

This study was conducted to evaluate the effects of three nutrient solutions (Hoagland, Knop, and Hydro Umat F) on the growth, yield, and fruit quality of hydroponically cultivated yardlong bean (Vigna unguiculata subsp. sesquipedalis L.). Results showed that Hydro Umat F extended the plant lifespan (105.4 days) and flowering time (58.65 days), increased the number of branches per plant, and enhanced leaf SPAD values. Plants grown in Hydro Umat F also exhibited the highest yield, as indicated by the number of flowers per plant (46.12), number of pods per plant (20.81), pod length (54.15 cm), pod weight (20.06 g), and total pod weight per plant (417.45 g). The Hoagland solution also promoted plant growth and yield, with a growth duration of 96.74 days, an average of 17.63 pods per plant, a pod length of 47.34 cm, a pod weight of 17.14 g, and a total yield of 302.17 g per plant. In contrast, the Knop solution, containing only six essential nutrients, shortened the plant’s growth duration (76.37 days) and significantly reduced the growth and yield of the hydroponic yardlong bean. Regarding pod quality, the pods from plants grown in Hydro Umat F had higher vitamin C content (4.12 mg/g), total protein content (3.21 mg/100 g), and reducing sugar content (13.06 mg/g) compared to those grown in Hoagland and Knop solutions. The findings suggest that Hydro Umat F is a suitable nutrient solution for hydroponic cultivation of yardlong bean, contributing to increased plant yield and fruit quality. These results suggest that Hydro Umat F is a promising nutrient solution for enhancing yield and pod quality in hydroponic yardlong bean cultivation, particularly in the context of the decline in available agricultural land and the rise of urban agriculture in Vietnam.

Comparison of experimental and numerical results on flow uniformity of seeds transmitted from the studded feed rollerOriginal Paper

Mustafa Gokalp Boydas

Res. Agr. Eng., 2024, 70(1):43-52 | DOI: 10.17221/34/2023-RAE

Studded feed rollers are widely used in seed metering units of seed drills. The flow evenness is an important indicator of the performance of studded feed rollers. With this research, the effects of studded feed rollers with different stud numbers (27, 36, and 45 studs) on flow evenness were investigated both in the laboratory and by simulation in case of using different ground speeds (1.5, 2, and 2.5 m·s–1). While the experiments were carried out on the seed drill model prepared in the laboratory, the simulation was done with the Rocky DEM software program. In the laboratory and simulation studies, it was determined that the flow evenness increased with the increase in the number of studs and the ground speed. The results obtained from the laboratory and simulation studies show parallelism with each other. However, it was seen that the results obtained in the laboratory were slightly higher than the results obtained from the simulation. With this study, it has been seen that it would be very beneficial to use the DEM model to improve the performance of the seed metering unit and to develop a new seed metering unit.

The content and potential ecological risk assessment of heavy metals in coastal wetlands around the Bohai SeaOriginal Paper

Yingchun Lv, Mengyi Wang, Wanxin Tian, Haibin Hui

Plant Soil Environ., 2024, 70(6):356-365 | DOI: 10.17221/24/2024-PSE

Coastal wetlands play a vital role in the migration and transformation of heavy metal pollutants in watersheds. There were 30 surface sediment samples that were analysed to investigate the distribution and ecological risks of heavy metals in the coastal wetlands around the Bohai Sea. Our results showed that the average concentrations of Pb, Cr, Ni, Cu, Zn, and Cd in these wetlands were 17.92 ± 5.81, 50.29 ± 20.50, 31.53 ± 9.71, 25.37 ± 4.29, 80.13 ± 15.11, and 0.92 ± 0.54 mg/kg, respectively. Relative to other wetlands, Pb (25.43 ± 2.68 mg/kg) and Cd (1.67 ± 0.06 mg/kg) contents were higher in the Liaohe Delta wetland (LHDW). Cu (28.44 ± 3.71 mg/kg), Cr (83.11 ± 5.80 mg/kg), and Ni (45.91 ± 3.02 mg/kg) contents were higher in the Yellow River Delta wetland (YRDW). The Zn (120.86 ± 7.41 mg/kg) content was higher in the Qilihai wetland. Heavy metal concentrations in coastal wetland sediments are shown to be positively correlated with organic matter content. Our results showed that the concentration of heavy metals decreases with increasing sediment particle size. In this study, Cd showed the highest pollution index and, therefore, more attention should be paid to the potential ecological risks of Cd in coastal wetlands around the Bohai Sea, especially in the LHDW and YRDW.

Commercial thyme essential oil as natural beverage preservative and molecular docking study on its mode of action against Saccharomyces cerevisiaeOriginal Paper

Zineb Lotmani, Mohamed Nadjib Boukhatem, Khaled Boudjema, Hamida Rezzoug, Amina Bouchra Benelmouffok, Pierre Tomi

Czech J. Food Sci., 2024, 42(4):263-272 | DOI: 10.17221/102/2024-CJFS

The present investigation explored the possible use of Thymus vulgaris essential oil (TVEO) as a beverage antifungal preservative instead of chemical ones. The chemical profile of TVEO exposed carvacrol (60.47%) as the predominant compound. The antifungal properties of TVEO were assessed on various food spoilage yeast and mould species using two tests. TVEO showed a powerful antimicrobial effect against all the fungal strains at the three volumes of essential oil (EO) used (i.e. 10, 20, and 30 µL). The minimum inhibition concentration (MIC) of TVEO was also evaluated and ranged from 0.0625% to 0.015% (v/v). Furthermore, the potency of TVEO as a beverage antimicrobial preservative was tested at four distinct concentrations (0.6, 1.25, 4, and 6 μL·mL–1) against Saccharomyces cerevisiae alone and combined with medium heating (70 °C for 2 min) in a real food matrix (Orangina® drink) for eight storage days. TVEO exhibited a significantly higher preservative effect than chemical preservatives (sodium benzoate and potassium sorbate). Lastly, a molecular docking examined the mechanism of action of carvacrol against two crucial enzymes in S. cerevisiae viability [ERG2 (sterol C8-isomerase) and ERG3 (sterol C5-desaturase)] compared to a chemical preservative (potassium sorbate). The two ligands highly interacted with the two target enzymes. However, carvacrol achieved a better score than potassium sorbate against ERG2 and ERG3, with binding energy of –10.19 kcal·mol–1 and –11.73 kcal·mol–1, respectively. Our results open up the perspective of using TVEO as a natural food preservative.

Agronomic and pod traits in relation to pod shattering in cultivated soybeansOriginal Paper

Bingjie Tu, Qingying Zhang, Xiaobing Liu, Shaopeng Yu, Nan Xu, Jia Liu, Changkai Liu

Czech J. Genet. Plant Breed., 2025, 61(2):67-76 | DOI: 10.17221/119/2024-CJGPB

Pod dehiscence or pod shattering from mature soybean (Glycine max L.) is one of the most outstanding disadvantages in domesticated cultivars. Pod shattering in relation to 16 quantitative traits and 3 qualitative traits among 140 cultivars of vegetable soybeans, grain soybeans and small-grain soybeans was evaluated over two years. We found the pod shattering percentage is positively correlated with the number of productive branches, pod width, pod length, pod area, 100-seed weight, 1-seeded-pod percentage, 2-seeded-pod percentage and seed protein content, but negatively correlated with the plant height, pod height at the bottom, number of nodes on the main stem, 3-seeded-pod percentage, 4-seeded-pod percentage and seed oil content. The pod shattering percentage in vegetable soybeans is remarkably high, reaching up to 93%, 7.8 times higher than that of grain soybeans. A schematic model of the characteristics for shatter-susceptible and shatter-resistant soybean cultivars is proposed. The pod shattering in vegetable soybeans is related to the “umbrella-shaped” architecture and pod size. It is suggested to select lines with more 2-seeded and 3-seeded pods for vegetable soybeans, but a higher seed oil content and greater node number on the main stem for grain soybeans and small-grain soybeans, to avoid pod shattering in future breeding programmes.

Kinetics and mathematical models of date paste dried using a convective infrared dryerOriginal Paper

Mahmoud Younis, Khaled Abdel Wahed Ahmed, Isam Ali Mohamed Ahmed, Hany Mohamed Yehia, Diaeldin Omer Abdelkarim, Ahmed Elfeky

Czech J. Food Sci., 2024, 42(6):465-475 | DOI: 10.17221/66/2024-CJFS


Achieving the desired level of caramelisation in a date powder requires considerable effort. Consequently, an assessment was conducted on efficacy of thin-layer infrared dehydration for date paste. Various parameters were considered, including airflow velocities of 0.5, 1.0, and 1.5 m·s–1, radiation intensities of 0.076, 0.1528, and 0.228 W·cm–2, and date paste layer thicknesses of 3 mm and 5 mm. The study's findings indicated a positive correlation between drying rate and lowering airflow velocity and a negative correlation between drying time and decreasing airflow velocity, the thickness of date paste, and rising intensity of infrared. The effective moisture diffusivity (Deff) was caculated by employing Fick's diffusion equation. For all situations under investigation, the mean values were within the range of 3.94 × 10–11 m2·s–1 to 6.01 × 10–10 m2·s–1. A relationship has been established between Deff and moisture content. Seven distinct mathematical models were subjected to rigorous validation by applying non-linear regression analysis, aiming to accurately characterise the drying process of date paste. The modified two-term model provided the most accurate forecast of the drying process for date paste layers.

Influence of phosphite and phosphate fertilizers at three different pH levels under floating system on the growth, yield, and nutrient concentration of broccoliOriginal Paper

Amin Jahanian, Seyed Jalal Tabatabaei, Nosratollah Najafi, Martina Bučková, Farzad Rasouli, Jiří Mlček, Sezai Ercisli

Hort. Sci. (Prague), 2025, 52(2):120-130 | DOI: 10.17221/16/2024-HORTSCI

This study was conducted to determine the effects of phosphite and phosphate fertilisers on broccoli’s yield, growth, and nutritional status (Brassica oleracea L. var. italica cv. ‘Fiorentino’). In factorial combinations, experiments were conducted at three pH levels (5.5, 7.0, and 8.5) and three phosphorus source levels [Pi (phosphate), Phi (phosphite), and Pi + Phi]. Phi had a statistically significant negative effect on yield, producing immature and button-like flower heads. Decreases in chlorophyll index, Fv/Fm [ratio of variable fluorescence (Fv) to maximum fluorescence (Fm)], and leaf area were observed using the Phi treatment. These results indicate that fertilisation with Phi alone did not affect plant growth and yield. P (phosphorus) and K (potassium) concentrations in plants were increased by root fertilisation with Phi (floating system), but did not affect growth characteristics; increased Pi uptake in Phi-treated plants increased P and K concentrations in the sink source, resulting in reduced growth, phytotoxicity, and no head formation. While it may appear that Phi is upsetting the balance of solution nutrients and stressing the plants, the Phi-induced stress condition was identified by measuring proline levels and electrolyte leakage. Thus, phosphite could not be used as a P source for plants. However, Phi could be used as a plant nutrient source combined with conventional Pi fertilisers.

Genome-wide identification and in silico expression analysis of CCO gene family in Citrus sinensis (orange) in response to citrus greeningOriginal Paper

Sadaf Sarwar, Muhammad Shafiq, Layba Tasawar, Iram Gull, Jannat Akram, Maha Al-Roshdi, Gabrijel Ondrasek, Muhammad Shafiq Shahid

Czech J. Genet. Plant Breed., 2025, 61(3):113-127 | DOI: 10.17221/11/2025-CJGPB

Citrus sinensis (L.) Osbeck (sweet orange) is the most important cultivated citrus fruit in the world. However, Hanglongbing (HLB) disease, caused by Candidatus Liberibactor asiaticus (CLAs), poses a major threat to sweet orange production, by hindering colour, quality and export. Carotenoid cleavage oxygenases (CCOs), which include carotenoid cleavage dioxygenases (CCDs) and 9-cis-epoxycarotenoid dioxygenases (NCEDs), are essential for plant growth, development, and adaptation to phytohormonal, biotic, and abiotic stresses. This study identified 14 CsCCO genes in C. sinensis. Structural and conservation studies were conducted using gene structure and conserved domain analysis. Genomic localisation, gene duplication, and similarity among these genes were also examined. Gene ontology analysis predicted that CsCCOs could be involved in the carotene catabolic process. Analysis of cis-regulatory elements revealed that most CsCCO genes are involved in responses to stress, light signalling, and plant growth regulation. Genes in the 9-cis-epoxycarotenoid dioxygenase (NCED) subgroup are predominantly localised in chloroplasts, whereas genes in other subgroups are primarily found in the cytoplasm. All 13 of the CsCCOs genes identified were regulated by 25 microRNAs, indicating the crucial role of microRNAs in gene regulation in Citrus sinensis. The expression patterns of CsCCO genes in response to biotic and abiotic stress were studied. Transcriptome analysis demonstrated that CsNCED3 and CsNCED10 were up-regulated in response to HLB. This provides insight into the function of CCO genes in C. sinensis and identifies potential candidate genes for combating citrus greening.

Chlorhexidine dihydrochloride's effect on clinical, veterinary and food-origin Staphylococcus aureusOriginal Paper

Marta Štindlová, Václav Peroutka, Simona Lencová, Kamila Zdeňková

Czech J. Food Sci., 2025, 43(5):326-335 | DOI: 10.17221/201/2024-CJFS

Chlorhexidine (CHX) is a bactericidal agent used as a common disinfectant since the 1950s. However, its effectiveness may have diminished over the time due to the rise of microbial resistance even among nonantibiotics. In this study, we evaluate the response of 46 Staphylococcus aureus isolates to CHXdihydrochloride according to their origin and phenotype (haemolysis induction, coagulase production, methicillin resistance and biofilm formation). Following classification, the influence of seven CHX concentrations (10.00–0.50 mg·L–1) on planktonic cell growth and biofilm formation was evaluated spectrophotometrically at 620 nm and 595 nm (24 h). Even though the effect of CHX was strain-specific irrespective of origin or phenotypic profile, concentrations above 2.50 mg·L–1 were almost uniformly determined as bactericidal. Although the non-bactericidal concentrations did not indicate any statistically significant differences, they did promote biofilm formation in some cases. Overall, our results suggest that CHX is still an effective disinfectant and an antimicrobial agent against S. aureus.

Species-specific responses of wheat and maize to thallium stress under elevated CO2: effects on yield, photosynthesis, and metabolismOriginal Paper

Samy Selim Abdelsalam, Soad K. Al Jaouni, Seham M. Hamed, Emad A. Alsherif, Afrah E. Mohammed, Modhi O. Alotaibi, Danyah A. Aldailami, Wael A. Obaid

Plant Soil Environ., 2025, 71(9):666-679 | DOI: 10.17221/328/2025-PSE

Heavy metal stress inhibits plant growth, but this impact is less studied and pronounced under climate change conditions. The present study investigates the physiological, biochemical, and agronomic responses of wheat (C3) and maize (C4) exposed to varying thallium (Tl) stress (60 and 120 mg/kg) under ambient (aCO2) and elevated (eCO2, 710 µmol/mol) CO2 levels. High Tl exposure markedly reduced grain yield by 58% in wheat and 68% in maize at 120 mg/kg under aCO2. However, eCO2 partially offset the negative effects, increasing yield by ~20% in wheat and 36% in maize at 60 mg/kg Tl. eCO2 enhanced photosynthetic activity under eCO2, which increased the accumulation of soluble sugars under TI stress. These provide carbon skeletons for the synthesis of primary metabolites such as amino acids, organic acids and fatty acids. Although total fatty acid content declined under stress, the metabolic crosstalk initiated by improved photosynthesis and sugar availability enables plants to maintain key fatty acids (such as palmitic, linolenic, and oleic acids) essential for membrane stability and function. Amino acids, especially proline and cysteine, accumulated significantly under Tl stress. These primary metabolites, in turn, feed into secondary metabolic pathways, promoting the formation of phenolic acids and flavonoids that enhance antioxidant defence and stress tolerance. This metabolic cascade explains eCO2’s capacity to alleviate TI stress and improve crop performance, and underscores the value of leveraging eCO2 environments to support agricultural productivity and food security under challenging conditions.

Sensory properties of pork sausage after sea buckthorn extract additionOriginal Paper

Marek Bobko, Lukáš Jurčaga, Alica Bobková, Alžbeta Demianová, Melina Korčok, Judita Lidiková, Miroslav Kročko, Ondřej Bučko, Andrea Mendelová, Terézia Švecová, Andrea Mesárošová

Czech J. Food Sci., 2025, 43(5):320-325 | DOI: 10.17221/11/2025-CJFS

The meat industry is actively trying to replace synthetic antioxidants with natural counterparts. Sea buckthorn is an excellent source of polyphenols and its high antioxidant properties. Various authors tested the antioxidation effect of sea buckthorn on lipids in meat products. The problem of sea buckthorn addition to food is its effect on sensory parameters. Our study incorporated sea buckthorn extract into pork sausage, stored for 21 days, and observed sensory properties and their changes. We mechanically measured the pH, colour, and textural properties of pork sausage samples with minimal variability observed. Also, sensory analysis by informed panellists was conducted on multiple storage days. Sensory evaluation revealed significant deterioration of sensory quality. Sea buckthorn remains an interesting option for the meat industry. However, further multidisciplinary research is still needed.

Colostrum quality in Northern Italy: The role of dry cow nutrition and colostrum feeding managementOriginal Paper

Martina Pavesi, Giulia Gislon, Serena Bonizzi, Stefania Colombini, Nicola Palladini, Maddalena Zucali

Czech J. Anim. Sci., 2025, 70(11):459-466 | DOI: 10.17221/104/2025-CJAS


This study investigated the effects of dry cow feeding on colostrum quality. A survey was conducted, and samples of colostrum and feed ingredients were collected from 25 dairy farms in Northern Italy. Colostrum was analysed for quality in terms of Brix value and gamma globulins, while diets and feed ingredients were analysed for their chemical constituents. The mean colostrum quality (n = 163) was 24.3 ± 4.41% Brix and 54.2 ± 20.8 g/l gamma globulin. The refractometer method effectively assessed colostrum quality, correlating accurately with laboratory gamma globulin measurements (R = 0.729). Factor analysis indicated that key nutrients, such as protein and fat daily intakes in dry cow diets, influenced colostrum immunoglobulin G. Furthermore, supplementation of selenium, vitamin A, vitamin B12, and vitamin E also improved colostrum quality. Colostrum feeding occurred, on average, 5.44 ± 2.63 h post-calving, with a quantity of 2.74 ± 0.71 l per meal, which is below the recommended 10% of the calf body weight. However, the maximum feeding time was 12 h, and the minimum colostrum quantity was 1.25 l, highlighting considerable room for improvement.

Enhancing vitamin C stability through liposomal encapsulation with optimised pressure and cycle conditionsOriginal Paper

Özlem Üstün-Aytekin

Czech J. Food Sci., 2025, 43(6):419-427 | DOI: 10.17221/42/2025-CJFS

Encapsulation technology offers an effective strategy to enhance the bioavailability and stability of vitamin C by addressing its sensitivity to environmental factors. This study investigates the impact of formulation parameters, particularly lecithin concentration and high-pressure processing conditions, on the physicochemical properties, gastrointestinal stability, cytotoxicity, and shelf life of liposomal vitamin C formulations. Among the tested samples, Sample 1, prepared with 20% soybean lecithin and 20% ascorbic acid and processed at 400 bar with a single cycle, demonstrated superior performance. It exhibited a high zeta potential (−23.17 mV), uniform size distribution (317.5 ± 8.863 nm) and encapsulation efficiency of 77.6%, along with 85% vitamin C retention under simulated gastrointestinal conditions. Cellular uptake in Caco-2 cells reached 30%, and structural integrity was preserved for 240 days at 40 °C, indicating strong thermal stability. The results underscore that lecithin concentration had the most significant influence on encapsulation efficiency and liposome stability, compared to pressure intensity or the number of processing cycles. Furthermore, modulating the zeta potential through lipid composition and the energy applied to phospholipid solutions was found to be critical for improving bioavailability and ensuring long-term dispersion stability. In conclusion, the optimised liposomal formulation offers a promising vehicle for advanced vitamin C delivery with enhanced protection, bioaccessibility, and storage potential.

Deconstruction of China's agricultural bioeconomy policies in the context of sustainable systems transition – Based on policy texts analysisOriginal Paper

Xiuxiang Li, Yanhan Hu

Agric. Econ. - Czech, 2025, 71(12):664-679 | DOI: 10.17221/423/2024-AGRICECON

Countries are calling for a sustainable transition of agri-food systems due to the volatility of food security in the context of climate change, and the agricultural bioeconomy may be a more dependable solution. By capturing, coding, and displaying word clouds of Chinese agricultural bioeconomy policy texts, this study deconstructs the development framework of China's agricultural bioeconomy policies based on the agricultural biosystem dimension, the policy instrument dimension, and the comprehensive dimension. It concludes that there is a clear trend of sustainable transformation and cross-sectoral linkage in China's agricultural bioeconomy policies. Overall, China's agricultural bioeconomy policies have the following comprehensive features: first, the policy is deeply integrated, considering both economic development and environmental friendliness; second, the policy is safety-oriented, taking into account competitiveness and strategy; and third, the policy is innovation-driven, taking into account fundamentality and foresight. Efforts should be made in the future to enhance the competitiveness of the agricultural bioeconomy within the framework of an all-encompassing approach to food and to help China's agricultural bioeconomy on the road to sustainable transition by improving fiscal and tax support, advocating the use of financial instruments, creating an alliance for the agricultural bioeconomy, and promoting international trade exchanges and cooperation.

Soil pore structure and its research methods: A reviewReview

Nannan Wang, Tibin Zhang

Soil & Water Res., 2024, 19(1):1-24 | DOI: 10.17221/64/2023-SWR

Soil pore is an important part of soil structure. According to the causes of formation, soil pores can be divided into biological pores formed by animal movement and plant root development and non-biological pores formed by dry-wet and freeze-thaw alternation or artificial tillage. The soil pore structure affects the migration of water, gas, nutrients and so on in the soil, especially the macropores can also produce water or solute preferential migration. Studying soil pores is of great significance for predicting soil hydraulic properties, reducing groundwater pollution and soil nutrient loss. Based on previous studies on soil pore structure, this paper systematically summarized the role of soil pores, influencing factors and the advantages and disadvantages of various research methods. This paper not only introduces traditional methods (including direct and indirect methods), but also summarizes the new research on soil pores combined with computed tomography (CT) technology and other science and technology in recent years. Finally, the prospect and development trend of soil pore research in the future were predicted, so as to provide reference for further research on soil pore structure.

Applying the European Union (EU) assessment initiative of forest sustainability in Africa: A case study of the timber harvesting impact on the environment in Sierra LeoneOriginal Paper

Petra Hlaváčková, Sheku Kemoh Mansaray, Samuel Antwi Darkwah, Iva Živělová

J. For. Sci., 2024, 70(2):79-90 | DOI: 10.17221/74/2023-JFS

The European Union (EU) provides both financial and expert support for sustainable forest management initiatives and also boosts compliance with such initiatives in Africa. Thus, there is a need to monitor the progress of implementing such initiatives. The objective of this paper is to describe the role of timber harvesting entities in environmental impact discussions, and to also identify the role of EU institutions in promoting forest sustainability in Sierra Leone. Data were collected in a controlled survey from four communities, using a questionnaire formulated according to guidelines recommended by Babbie and Mouton (2010). The reliability test shows a 0.68 internal consistency among the latent variables (Cronbach alpha test). The selected four communities were Bambawo, Dia, Basara, and Menima, with a population of 6 000, 5 400, 7 500, and 5 600 inhabitants, respectively (Stats SL 2021). A Confirmatory Factor Assessment (CFA) was used for a descriptive statistical analysis of the data. The results showed that the environmental impact has an eigenvalue of 1.08, where only variables with a factor loading exceeding 0.5 were used. The paper concludes with discussions and relevant recommendations on mitigating the impacts of timber harvesting on the environment within the study area.

Enhancing cattle production and management through convolutional neural networks. A reviewReview

Jean de Dieu Marcel Ufitikirezi, Roman Bumbálek, Tomáš Zoubek, Petr Bartoš, Zbyněk Havelka, Jan Kresan, Radim Stehlík, Radim Kuneš, Pavel Olšan, Miroslav Strob, Sandra Nicole Umurungi, Pavel Černý, Marek Otáhal, Luboš Smutný

Czech J. Anim. Sci., 2024, 69(3):75-88 | DOI: 10.17221/124/2023-CJAS

The rise in demand for animal products associated with global population growth has driven the world toward precision livestock farming, where convolutional neural networks (CNN) have gained increasing attention due to their potential to enhance animal health, productivity, and welfare. However, the effectiveness and generalizability of CNN applications in cattle production are limited by several challenges and limitations, which require further research and development to address. This systematic literature review aims to provide a comprehensive overview of the applications of CNN in cattle production. It identified some potential applications of CNN in this field and highlighted the challenges and limitations that need to be addressed to improve the effectiveness and efficiency of CNN applications in cattle production. It also provides valuable insights for researchers, practitioners, and policymakers interested in the use of CNN to enhance cattle production practices, animal welfare, and sustainability. Additionally, it also provides the reader with a summary of the literature on the fundamental concepts of convolutional neural networks and their commonly used model architectures in cattle production. This is because agriculture digitalisation is going more multidisciplinary and people from different areas of expertise may find it helpful to learn more from a combined source.

Molecular detection and characterisation of Mycoplasma species in community owned dogs of Kerala, a South Indian StateOriginal Paper

GS Kumar, A Varghese, PK Hembram, KGA Kumar, CK Deepa, A Varghese, R Ravindran

Vet Med - Czech, 2024, 69(9):321-328 | DOI: 10.17221/116/2023-VETMED

Haemotropic mycoplasmas (haemoplasmas) are obligate epierythrocytic bacteria that infect a wide range of vertebrate hosts. The molecular characterisation of Mycoplasma organisms in dogs has not previously been attempted from India. Hence, in the present study, the molecular characterisation of Mycoplasma spp. in dogs of different zones of Kerala was attempted using 16S rRNA gene. An overall prevalence of 18% for M. haemocanis was detected. The NCBI-BLAST analysis of all the selected sequences revealed > 99% identity with the sequences of M. haemocanis. The phylogenetic analysis revealed clustering of M. haemocanis and M. haemofelis in a single clade indicating low genetic variability. It was further supported by the genetic distance data and haplotype analysis.

Potential for monetisation and utilisation of wood raw material from pioneer tree species stands in the Czech RepublicOriginal Paper

Roman Dudík, Petra Palátová, Aleš Kůs, David Homolka, Andreas Nikodemus

J. For. Sci., 2025, 71(2):99-111 | DOI: 10.17221/101/2024-JFS

Pioneer tree species in the Czech Republic include birch, alder and aspen. From an economic point of view, birch seems to be the most promising. Greater use of timber from pioneer tree species in higher value-added products is currently limited by the low stocks of birch, alder and aspen in forest stands. A price survey was carried out in the Western Highlands region in the central part of the Czech Republic to obtain basic information on the prices of assortments of pioneer tree species. The prices of five assortments of raw timber were surveyed for the period 2019–2023. Very few of the prices of pioneer tree species were found for assortments of II and IIIC; these assortments are not actually produced and, therefore, not traded by the respondents. For the roundwood assortments (quality II, IIIAB), it cannot be clearly stated that any of the preparatory timber species (birch, alder, aspen) always achieved a higher price during the observed period. Only in 2023 was it possible to see higher prices for quality IIIAB assortments of alder. Birch of quality V had the highest price of the observed prices for this timber assortment for the pioneer tree species during the period under review. When using a system approach, considering the information on the prices of the timber assortments is also important for deciding on the optimal species composition of the regenerating forest stands. In the case of the Czech Republic's commercial tree species, this information is available at the national level. However, the situation is quite the opposite for the timber assortments of pioneer tree species, where information on their prices is completely lacking. This confirms the fact that the market for assortments of timber from pioneer tree species in the Czech Republic is basically non-existent, the main reason being the low volume of these assortments placed on the market.

Study on the main physicochemical characteristics of different plant cultivation substrates and their effects on standard rosesOriginal Paper

Yingguo Wang, Tingting Cao, Juan Li, Hang Zhou, Haiou Zhang

Plant Soil Environ., 2024, 70(12):799-808 | DOI: 10.17221/258/2024-PSE

Standard roses are a widely used ornamental plant in urban landscapes, valued for their attractive flowers and adaptability to various environmental conditions. This study investigated how different substrate types affect the growth and development of standard roses and their potential to improve the ecology of urban landscapes. Nine substrate conditions (rotted corn stover, decomposed shiitake mushroom residue, perlite, and combinations) were compared with field soil as a control treatment. The physical and chemical characteristics of each substrate were analysed, and the growth and development of standard rose plants were observed over six months. The results indicated that the substrate T4 (70% rotted corn stover, 15% decomposed shiitake mushroom residue, 15% perlite) achieved the highest evaluation index, leading to superior plant growth compared to other substrates. This combination provided optimal water retention, aeration, and nutrient supply, making it the most effective substrate for cultivating standard roses. Additionally, the use of these substrates can improve soil quality and reduce environmental pollution, offering a sustainable option for urban landscape management.

Ecophysiological aspects of some sweet cherry cultivars from the North-East of RomaniaOriginal Paper

Iulia Mineata, Ionel Perju, Sorina Sîrbu, Iuliana Elena Golache, Ionut Vasile Ungureanu, Stefanica Ostaci, Carmen Doina Jitareanu

Hort. Sci. (Prague), 2024, 51(4):305-313 | DOI: 10.17221/113/2023-HORTSCI

The physiological performance, growth and competitive ability of fruit trees are increasingly affected by the effects of global climate change, very different at a regional level, which mainly causes considerable changes in water availability. The purpose of this study was the evaluation of three sweet cherry cultivars from the Research Station for Fruit Growing (RSFG) Iași, Romania under the climatic conditions of 2022–2023 by performing physiological investigations into the water regime and the transpiration process through the stomatal conductance, the water content of the leaves as well as their dehydration rate after 24 hours. The obtained data were statistically interpreted taking three experimental factors into account: factor A consisted of three distinct phenological stages (full flowering, fruit about 80% of the final size and fruit ripening); factor B constituted by the three cultivars (‘Van’, ‘Andreiaș’ and ‘Margonia’); and factor C was constituted by the canopy area from the samples (internal and external). The experimental variants statistically interpreted by Duncan’s test (P ≤ 0.05) registered significant differences. The Pearson correlation coefficient (R2) between the measured variables obtained positive distinctly significant values of R2 = 0.686 (with the stomatal conductance) and negative distinctly significant values of R2 = –0.874 (with the water content). The obtained results will support the development of predictive models for different irrigation and breeding strategies to improve the sweet cherry production in temperate continental climates.

Effects of natural plant growth regulator iron chlorin on photosynthesis, yield, and quality of watermelons grown in greenhousesOriginal Paper

Qian Feng, Lu Lu, Qingyun Li, Liu Wang, Qingyu Pei, Shoujiang Pan, Jing Tian, Shaowei Lu, Shao Li

Hort. Sci. (Prague), 2024, 51(4):341-347 | DOI: 10.17221/132/2023-HORTSCI

Iron chlorin is known to affect plant growth, but its potential applications in watermelon production have rarely been explored. To better understand its effects on the growth, photosynthesis, yield, and quality of watermelon in a greenhouse setting, a series of experiments were conducted using the variety ‘Sumeng 6’. At the flowering and early fruit expansion stages, the plants were sprayed with iron chlorin with mass concentrations of 0.001, 0.002, 0.004, and 0.008 μg/L (T1, T2, T3, and T4). Control plants were sprayed with water (CK). The growth index, root activity, photosynthetic pigment content, photosynthetic parameters, yield, and fruit quality of all plants were measured. The results showed: compared with CK, the T3-treated plants showed significant improvements in several aspects: the leaf contents of photosynthetic pigments, chlorophyll a, total chlorophyll, and carotenoid, increased by 19.51%, 14.29%, and 29.17%, respectively (P < 0.05); the net photosynthetic rate (Pn) increased by 23.60% (P < 0.05); and the soluble solids content, vitamin C content, and yield increased by 7.89%, 34.13%, and 16.27%, respectively (P < 0.05). In summary, it was found that spraying 0.004 μg/L iron chlorin on facility watermelon plants at the flowering and the early fruit expansion stages has a significant effect on the promotion of growth and development, leading to improved quality and yield. This study provides a theoretical reference and technical guide for high-quality and efficient watermelon production.

The potential of volatiles from Brassica juncea seeds against grey mould agent Botrytis cinerea and their effect on storage and sensory quality of spinach leavesOriginal Paper

Beata Kowalska, Magdalena Szczech, Maria Grzegorzewska, Anna Wrzodak, Kalina Sikorska-Zimny

Plant Protect. Sci., 2025, 61(1):66-76 | DOI: 10.17221/44/2024-PPS

The potential use of volatile compounds released from milled seeds of mustard (Brassica juncea cv. Malopolska) obtained from three different companies was tested in in vitro and in vivo experiments for their inhibitory effect on Botrytis cinerea growth on agar media and its infection on vegetable leaves of cucumber, bean and spinach. In the experiments with spinach, the effect of volatiles from mustards on the storage and sensory quality of fumigated leaves was evaluated. The antifungal effect of the volatiles depended on the source and dosage of mustard seeds and biofumigation time. The most efficient inhibition of B. cinerea mycelium growth on agar media and vegetable leaves was mustard S from SHR company. The development of grey mould on spinach leaves was inhibited in the treatment with 4 h biofumigation with the volatiles from mustard S seeds in experiments conducted at 10 °C and also at 18 °C. In the sensory and storage quality analysis, the spinach leaves treated with volatiles from mustard seeds showed acceptable parameters that predisposed the product to consumption. The results show that it is possible to reduce the incidence of vegetable grey mould with the treatment of milled mustard seeds, opening a potential application of biofumigation in the control of B. cinerea in vegetables.

Effects of exogenous 2, 4-epibrassinolide and its inhibitors on the seed setting and yield of Tartary buckwheatOriginal Paper

Peiuyn He, Huangdanni Ran, Kaifeng Huang

Plant Soil Environ., 2025, 71(3):161-170 | DOI: 10.17221/669/2024-PSE

The aim of this study was to evaluate the effects of exogenous 2, 4-epibrassinolide (EBR) on the yield of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.). A 2-year field experiment was conducted on Tartary buckwheat (cv. Jinqiao 2) with different concentrations (0, 0.1, 0.5, 1.0, and 2.0 mg/L) of EBR and brassinolide (BRZ, inhibitor of brassinolide synthesis). The seed setting rate, agronomic traits, and yield initially increased and then decreased with an increase in the EBR application rate. The seed setting rate, agronomic traits, and yield decreased gradually with an increase in BRZ concentration, and yield was the lowest at 2.0 mg/L. The appropriate application of exogenous EBR could promote the increase of Tartary buckwheat yield. Compared with 0 mg/L (control), the 0.1, 0.5, and 1.0 mg/L treatments increased yield by 13.53, 32.73, and 7.08%, respectively, while the high-concentration treatment (2.0 mg/L) decreased by 4.13%. In conclusion, the appropriate concentration of EBR treatment (0.5 mg/L) delayed the senescence of Tartary buckwheat by increasing its root activity and the activity of antioxidant enzymes in leaves. Simultaneously, it increased the chlorophyll content of Tartary buckwheat leaves, enhanced photosynthesis, increased nonstructural carbohydrate content, and augmented the "source," increasing the seed setting rate and yield of Tartary buckwheat. This concentration is recommended for use in the production of Tartary buckwheat.

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