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Results 631 to 660 of 4099:

Determinants of pollutant emissions in the Spanish agri-food sector: The role of international tradeOriginal Paper

Yolanda Sorroche-del-Rey, Laura Piedra-Muñoz, Emilio Galdeano-Gómez, Eva M. García-Granero

Agric. Econ. - Czech, 2025, 71(3):130-141 | DOI: 10.17221/353/2024-AGRICECON

This study aims to analyse the key company-level variables influencing pollutant emissions in the Spanish agri-food sector and investigate the bidirectional relationship between international trade and environmental performance. Using panel data from 2007–2020, we employ discrete choice models to test causal relationships between business variables and environmental impact. Empirical findings show a negative correlation between internationalisation and polluting emissions from agri-food companies. Additionally, other factors, such as company age and size, also influence this index. These results provide valuable insights for economic decision-makers in the agri-food market, highlighting the implications of international trade and business variables on pollution levels.

Understanding societal priorities for forest ecosystem services: Survey insights from 'Forestry Days 2024' in SlovakiaOriginal Paper

Zuzana Sarva¹ová, Jozef Pajtík, Zuzana Dob¹inská

J. For. Sci., 2025, 71(4):195-204 | DOI: 10.17221/7/2025-JFS

Understanding society's demand for forest ecosystem services (FES) is crucial for effective forest management and the development of supportive instruments, such as payments for ecosystem services (PES). This study surveyed visitors at 'Forestry Days 2024' in Slovakia through face-to-face questionnaires, capturing their views on FES and necessary management changes. Results show that regulating services and biodiversity are prioritised over cultural and provisioning FES. Respondents indicated a clear need for changes in forest management to reflect their FES preferences. Notably, perceptions of specific FES varied by gender, age, forestry background, and forest ownership but not by residence type. Public PES were considered the most acceptable policy for enhancing FES management, providing valuable insight for aligning public preferences with sustainable practices in Slovakia.

New knowledge in dragon tree researchReview

Petr Madìra, Fabio Attorre, Kay Van Damme

J. For. Sci., 2025, 71(5):205-217 | DOI: 10.17221/9/2025-JFS

Dragon trees, arborescent members of the genus Dracaena (Asparagaceae), provide a wide range of ecosystem services and have been ethnobotanically important plants since ancient times. Currently, their relictual distribution is fragmented, populations are isolated and often under threat. We provide a brief overview of scientific studies and the state of knowledge on dragon trees published since 2020, when the last review was published. More than 120 papers dealing with dragon trees have appeared, indicating a significant interest in their research, cultural uses and conservation. The most intensively investigated species are Dracaena cochinchinensis, D. cinnabari, D. draco and D. cambodiana. Almost half of the papers deal with the chemical composition of resin and its bioactivity, in addition to studies on ecology, conservation and genetics. Only a few studies are devoted to taxonomy and ethnobotany.

Forest certification management experience in Asia Pacific and Europe: A case study of Malaysia and SwedenOriginal Paper

Shah Badri Mohd Nor, Ahmad Fariz Mohamed, Shamsul Khamis

J. For. Sci., 2025, 71(6):269-285 | DOI: 10.17221/3/2025-JFS

The forest certification is a voluntary programme to ensure that the timber harvested from forests is processed in a sustainable manner by following specific standards. The Programme for the Endorsement of Forest Certification (PEFC) was introduced in 1999 using the bottom-up approach, and it gives flexibility for a country to set up their own PEFC standard based on current practices in sustainable forest management (SFM), regulation, and governance. This practice has led to the development of various PEFC standards for each country; thus, the impact of PEFC certification differs from country to country. In accordance with that, this study aimed to evaluate the PEFC standard application in developed and developing countries, namely in Sweden and Malaysia. The study also analysed the impact of forest certification on governance and economic aspects within Asia Pacific and Europe. The outcome of this study indicates that the PEFC standards developed by these countries showed similarities; for example, both countries are focusing on social and environmental requirements. Meanwhile, the differences were shown in the criteria, terms used, and the focus area. The impact analysis exhibited that countries are facing similar issues, especially market access and premium prices. This study revealed that each country develops different standards based on current practices, forest diversification, and regulations.

Effect of application of microbiological preparation and different nitrogen fertilisation on wheat yield elementsOriginal Paper

Suzana Kristek, Jurica Joviæ, Josipa Janto¹, Marina Martinoviæ, Zdenko Lonèariæ

Plant Soil Environ., 2025, 71(7):500-508 | DOI: 10.17221/18/2025-PSE

Two-year experiments were conducted on two different soil types during 2021/22 and 2022/23 to study the impact of microbiological preparations, Mycor-FAZOS and Vitality-FAZOS, on the yield and quality elements of winter wheat cultivar Maja (Agrigenetics). The experiment was set up in a split-block design with four repetitions across two soil types, with 12 different variants on each soil type. The base plot area was 40 m2, and the effective plot area was 27 m2. The research factors included: A (soil type): A1 – gley soil; A2 – eutric cambisol; B (disease control): B1 – control; B2 – chemical pesticides; B3 – biopreparates (seed treatment + foliar treatment) and C (nitrogen fertilisation): C1 – based on soil analysis; C2 – 70% of recommended application. Variables included grain yield (t/ha), grain protein content (%), hectoliter weight (kg), and plant height (cm). The highest average grain yield was achieved with variant B3C2, with a 12.50% increase compared to variant B2C1. Differences between these variants included 6.0% for protein content, 5.97% for hectoliter weight, and 7.93% for plant height. It was observed that Mycor-FAZOS seed treatment promoted root development, resulting in healthier, taller, more robust plants with a more uniform growth and darker colour than untreated seed variants (indicating increased photosynthesis index). The protection achieved with Vitality-FAZOS biopreparations (fungicidal microorganisms synthesising growth hormones) ensured healthier wheat plants, leading to higher seed yield and better quality parameters.

Haemotropic Mycoplasma species in cat blood samples by PCROriginal Paper

O Gulaydin, M Yesilyurt, G Akgul, O Erdeger, K Eercan

Vet Med - Czech, 2025, 70(8):294-301 | DOI: 10.17221/7/2025-VETMED

Haemotropic mycoplasmas can cause severe anaemia in pets and also have zoonotic potential. The determination of these microorganisms from clinical samples is of critical importance. In this study, the presence of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm), and Candidatus Mycoplasma turicensis (CMt) in blood samples collected from 62 cats was investigated. Haemotropic mycoplasmas were identified by PCR amplifying the 16S rRNA gene, and sequence analysis was applied for confirmation and differentiation of the species. Six (9.67%) blood samples were positive for haemotropic mycoplasmas. CMhm and CMt were identified in five (8.06%) samples and one (1.61%) sample, respectively. Mhf was not detected in the samples. Additionally, a co-infection was not observed in any of the cats. It was found that the PCR positivity was not related to the sex or clinical status of the cats. To our knowledge, this is the first report investigating haemotropic mycoplasmas in blood samples from cats in Siirt Province of Türkiye. It is suggested that the data obtained from this study will contribute to clinicians working on pet animal health in this province.

Prevalence of lumbosacral transitional vertebrae and association with hip dysplasia in Rhodesian Ridgebacks in GermanyOriginal Paper

SK Slunsky, P Slunsky, E Fort, L Brunnberg

Vet Med - Czech, 2025, 70(10):369-378 | DOI: 10.17221/30/2025-VETMED

Congenital anomalies affecting the spinal column are frequently observed in certain dog breeds. One such condition occurs at the junction between the lower back and the sacrum, where vertebrae exhibit mixed structural characteristics. This retrospective study analysed imaging records of Rhodesian Ridgebacks collected over 28 years, selecting only those that met strict positioning standards for evaluation. A total of 2 012 cases were analysed using established classification methods, identifying three distinct structural variations: a typical junction, a bilaterally altered morphology, and an asymmetrical form affecting one side. The prevalence of these variations was 7.4%, with symmetrical alterations found in 5% of cases and asymmetrical alterations in 2.4%. Statistical analysis did not reveal a significant association between these anatomical variations and canine hip dysplasia (CHD) (P = 0.170 0). These findings support previous research indicating that there is no direct link between these structural variations and joint disorders in this breed. The study represents the most extensive investigation of its kind in this canine population, highlighting that such vertebral variations are frequently observed in routine radiographic screenings, but despite decades of research, consistent clinical correlations remain elusive – underscoring the need for further systematic investigation.

Influence of nitrogen, sulphur, and selenium foliar application on yield and accumulation of selenium in spring wheat grains (Triticum aestivum L.)Original Paper

Ladislav Ducsay, Alexandra Zapletalová, Mária Vicianová, Ladislav Varga, Marek Slepèan, Marek Ra¹ovský, Vladimír Paèuta, Dávid Ernst, Peter Hozlár, Da¹

Plant Soil Environ., 2025, 71(10):750-758 | DOI: 10.17221/67/2025-PSE

The study is focused on the evaluation of selenium, nitrogen and sulphur effects on yield, macro- and micronutrient content (N, P, K, Ca, Mg, S, Zn, Fe, Mn, Cu) and quality (Se content, starch, fibre, ash and fat) in wheat grain. Small-plot field experiments (10 m2 each plot) were established on loam to clay loam mollic soil with total Se content 0.21–0.22 mg/kg in ®eliezovce on the land of the Central Control and Testing Institute in Agriculture of the Slovak Republic. The effect of growing season on two sources of selenium, in the form of sodium selenite (Na2SeO3 · 5 H2O) and sodium selenate (Na2SeO4), was monitored during the growth phase BBCH 29 (the end of the tillering phase) in a two-year experiment. The experiment included six foliar treatments in four repetitions, which were differentiated as follows: T1 – 30 kg N/ha; T1 Se032– – 30 kg N/ha and 20 g Se/ha; T1 Se042– – 30 kg N/ha and 20 g Se/ha; T2 – 30 kg N/ha and 10 kg S/ha; T2 Se032– – 30 kg N/ha, 10 kg S/ha and 20 g Se/ha; T2 Se042– – 30 kg N/ha, 10 kg S/ha and 20 g Se/ha. A statistically significant difference in yield was found between the growing seasons. Statistically non-significant impact of treatments on achieved yields was found. The highest average Se content in grain, 0.90 ± 0.28 mg/kg, was achieved on treatment T2 Se042–. The application of sodium selenite appeared to be less effective than selenate form in the evaluation of average Se content in grain, where statistically significantly higher Se contents (T1 Se042– 0.78 ± 0.22 mg/kg; T2 Se042– 0.90 ± 0.28 mg/kg) were found after selenate application. The application of two types of fertilisers and two forms of selenium did not significantly increase the content of N, P, Mg, and S in grain. The Fe content in the grain was increased by treatment T2 Se032–. The application of sodium selenate compared to sodium selenite significantly increased the starch content (T1 Se042– 56.39 ± 4.44%; T2 Se042– 55.87 ± 4.05) in the grain of spring wheat.

Nonlinear effects of bank loans on county agrifood SMEs innovation: Empirical evidence from ChinaOriginal Paper

Xi Chen, Kai Wang, Lirong Xing, Jianzhen Lu

Agric. Econ. - Czech, 2025, 71(11):604-617 | DOI: 10.17221/324/2024-AGRICECON

Innovation is the driver of sustainable business development and is essential to promote high quality econo-mic development in the country. Are more bank loans better for small and medium-sized enterprises (SMEs) innovation? Therefore, based on mixed cross-sectional data of county sweet potato processing enterprises, this study applied the econometric model to explore the impact of bank loans on county agrifood SMEs innovation. We find that there is an 'inverted U-shaped' relationship between bank loans and county agrifood SMEs innovation. The analysis of the mechanism shows that bank loans can not only alleviate the problem of innovation financing constraints for agrifood SMEs, but also provide financial support for the innovation activities of agrifood SMEs as a result of trade openness. This study has important practical implications for promoting county agrifood SMEs innovation in China and promoting high-quality county economic development.

Exploring the impact of potassium fertiliser rate and split ratio on rice yield and quality in China: a meta-analysisOriginal Paper

Lijuan Deng, Duoji Wu, Weiqi Yuan, Zongqiang Wei, Yanlan Huang, Zhihua Hu, Jianfu Wu

Plant Soil Environ., 2025, 71(12):891-904 | DOI: 10.17221/315/2025-PSE

Potassium (K) is crucial for rice yield and quality, but continuous yield increase reduces protein content, challenging the balance between high yield and quality. This study analysed 3 178 case studies (1994–2024) on K management impacts on rice yield, grain protein, and amylose content, evaluating effects of K fertiliser rates, base-topdressing ratios, planting regions, and soil properties. The results showed that K application significantly increased rice yield, protein content and amylose content by 11.6, 2.0 and 1.0%, respectively. Importantly, we identified targeted K fertilisation strategies tailored to different quality goals: optimising for eating quality, nutritional quality, or synergistic improvement of yield and comprehensive quality. This study provides a scientific basis for precision K management to help growers balance rice yield with specific quality needs.

Allometric coefficient in broilers and development of white striping and wooden breast myopathiesOriginal Paper

Fernando Alberto Benitez dos Santos, Claudia Marie Komiyama, Vivian Aparecida Rios de Castilho Heiss, Maria Fernanda Castro Burbarelli, Rodrigo Garófallo Garcia, Deivid Kelly Barbosa, Bruna Barreto Przybulinski, Leonardo de Oliveira Seno, Andrey Sávio de Almeida Assunção, Erika Rosendo de Sena Gandra

Czech J. Anim. Sci., 2023, 68(5):212-221 | DOI: 10.17221/182/2022-CJAS


The aim was to study the evolution and severity of white striping and wooden breast myopathies and to carry out a study on the parameters of meat quality and allometric growth of male and female broilers at different ages. The experiment was conducted in a broiler house in a completely randomized design, using 960 one-day-old chicks of the Cobb® strain, with 480 chicks of each sex. 96 birds per treatment were slaughtered weekly at the age of 21 to 49 days. The present study analyses the occurrence of myopathies, their scores, and their evolution over the age of the birds, comparing males and females. Further assessments included the allometric coefficient and meat quality analyses such as physical measurements (breast length, width, and thickness), pH, colour (L*, a*, and b*), drip loss, water-holding capacity, cooking loss, and shear force. The results demonstrate that the sex of birds did not affect the occurrence of white striping and wooden breast myopathies. However, the degree of myopathies evolved with advancing age, suggesting the weight as a predisposing factor for these myopathies. The emergence of white striping myopathy occurred as early as in the third week of the birds’ life. Wooden breast did not develop as isolated myopathy, occurring only in the concomitant presence of white striping. The allometric coefficient demonstrated that regardless of the sex and age, broiler breast had positive heterogonic growth, i.e. late growth in relation to the other parts of the body.

Synergism of plant microbe interactions for remediation of potentially toxic elementsReview

Ranjna Kaundal, Vipin Parkash, Supriti Paul, Meghna Thapa

J. For. Sci., 2023, 69(4):127-143 | DOI: 10.17221/1/2023-JFS


Industrialization and urbanization are important for economic development which makes the human life easy by providing different job opportunities, increasing the production level of cheaper goods and standard of living. Despite its many positive effects, industrialization has had a negative impact on the natural ecosystem through environmental pollution. It is responsible for a greater input of potentially toxic and non-toxic substances into essential environmental components such as air, soil and water. Continuous industrialization has resulted in significant environmental problems due to the release of pollutants and extremely difficult treatment of contaminated areas. This review focuses on the recent literature dealing with the role of Plant Growth Promoting Microbes (PGPMs), i.e. bacteria and Arbuscular mycorrhizal Fungi (AMF) in the remediation of polluted sites.

Sensitivity analysis and performance evaluation of neural networks for predicting forest stand volume – A case study: District 2, Kacha, Guilan province, IranOriginal Paper

Sima Lotfi Asl, Iraj Hassanzad Navroodi, Aman Mohammad Kalteh

J. For. Sci., 2024, 70(5):209-222 | DOI: 10.17221/111/2023-JFS

Tree volume is a characteristic used in many cases, such as determining fertility, habitat quality, growth size, allowable harvesting, and the principles of forest trade. It is imperative to develop methods that predict forest stand volume to obtain this extensive information quickly and cost-effectively. This study used supervised self-organising map (SSOM), multi-layer perceptron (MLP), and radial basis function (RBF) neural networks to predict forest stand volume based on physiography, topography, soil, and human factors. A sensitivity analysis method called the importance of prediction was used to determine how input variables influenced network output. First, the map of homogeneous units was prepared with ArcMap (Version 10.3.1, 2015) by combining digital layers to measure the tree's volume per hectare. Then, separate tree species in different diameter classes were measured in a circular grid of 200 m × 150 m, 0.1 ha of coverage, 3.3% sampling intensity, and a diameter at breast height (DBH) greater than 7.5 cm using systematic sampling on a homogeneous unit map in a regular random method. The neural network modelling results showed that SSOM, MLP, and RBF predicted forest stand volume most accurately according to physiography, topography, soil, and human factors. Furthermore, the sensitivity analysis results found that altitude above sea level, soil depth, and slope are the most influential input variables. In contrast, soil texture variables are the least effective at predicting forest stand volume.

Comparison of selected data acquisition models using on-farm production records on qualitative parameters of oocytes in dairy cowsOriginal Paper

Ludìk Stádník, Veronika Kinterová, Jiøí ©ichtaø, Jaromír Ducháèek, Matú¹ Ga¹parík, Lucie Nìmcová, Radek Procházka, Radim Codl

Czech J. Anim. Sci., 2024, 69(1):1-10 | DOI: 10.17221/156/2023-CJAS

Dairy cows enter the negative energy balance in the early post-partum period, which negatively affects milk yield, health status and reproduction. This study aimed to determine if milk yield (MY) and fat/protein (F/P) ratio could be used as reliable indicators for predicting the quality of aspirated oocytes and their further use for in vitro fertilisation. A secondary goal was to compare different periods before oocyte aspiration to determine which period had the most significant impact on the oocyte development in donor cows. All tested Holstein cows were at their first lactation (n = 68). The first ultrasound-guided follicular aspiration in donor cows was done on average at 58.7 days in milk (DIM). Usually, a second aspiration followed a week later if no problems were detected. In total, 102 aspirations were performed. Three model equations with different F/P ratio distributions and variants of MY production were tested in statistical evaluation. On average, 3.6 oocytes were retrieved per donor per aspiration, with a minimum of 1 and a maximum of 12. The maturation rate was 62.2%, with a high probability of reaching metaphase II (90.45%). The highest number of oocytes was obtained from low-yielding cows with a low F/P ratio in all evaluated periods (P < 0.01). The quality and expansion of cumulus-oocyte complexes were unaffected by the metabolic status. However, a tendency for better quality oocytes in donors with lower MY and F/P ratios was observed in all models. Our preliminary results showed that the prediction of oocyte quality in a homogeneous group of the first-lactation cows was related to the chosen data acquisition model. Data about milk yield and F/P ratio obtained from on-farm systems provide a valuable source of information for the selection of oocyte donors.

In vitro antifungal activity and mechanism of action of carvacrol against Sclerotinia sclerotiorum (Lib.) de Bary

Lei Yang, Xue Ma, Lijun Wang, Guogen Yang, Lijun Zhou, Zhilin Zhang, Xiaomeng Li

Plant Protect. Sci., 2024, 60(2):172-180 | DOI: 10.17221/121/2023-PPS

This study aimed to examine the antifungal effects of nine monoterpenes on Sclerotinia sclerotiorum via the mycelial growth rate method. The effects of carvacrol on the morphological structures of hyphae were investigated by scanning electron microscopy (SEM). The oxalic acid (OA), total protein contents, and the activity of cell wall-degrading enzymes, including chitinase, cellulase and β-1,3-glucanase, were assessed. The results showed that the antifungal rates of carvacrol, thymol and eugenol reached 100% at a concentration of 400 mg/L, and the EC50 values of carvacrol, thymol, and eugenol were 43.40, 56.22, and 86.63 mg/L, respectively. The treatment of S. sclerotiorum with carvacrol had no significant effect on sclerotia formation, but the mycelial surface was shrivelled, uneven and broken, with cytoplasm flowing out. The OA content of S. sclerotiorum was significantly reduced to 133.78 µg/mL after treatment with carvacrol. Additionally, the total protein content of S. sclerotiorum mycelia in the carvacrol treatment group was significantly reduced to 15.67 µg/mL compared with that of the control group, and the activity of cellulase in the carvacrol treatment group was significantly higher than that in the control group.

Environmental problems and biodiversity conservation through silviculture in Sri Lanka: Current state, struggles and cooperation with Czech foresters – A reviewReview

Channa Suraweera, Josef Gallo, Stanislav Vacek, Martin Balá¹

J. For. Sci., 2024, 70(6):281-298 | DOI: 10.17221/19/2024-JFS

Sri Lanka, as a developing tropical country, faces serious environmental challenges. Forests, as important ecosystems of Sri Lanka, are under pressure from intensive human activities and climate change. Invasive species represent a great hazard to autochthonous plant species and ecosystems, which are, at the same time, hosts to extraordinarily high biodiversity, of which a high percentage is endemic. In this paper, we reviewed the most challenging environmental problems of Sri Lanka and suggested the improvement of a number of them through silvicultural measures and cooperation with international experts, namely Czech foresters. We also reviewed the main forest biomes and addressed the specific problems and management of different forest types.

Selectivity and efficiency: evaluation of synthetic Grapholita molesta sexual pheromonesOriginal Paper

®aneta Pra¾anová, Hana ©efrová

Plant Protect. Sci., 2024, 60(3):267-277 | DOI: 10.17221/47/2024-PPS

The effectiveness of pheromone lures for Grapholita molesta (Busck 1916) from Propher (Czech Republic) and Pherobank (Netherlands), the attractiveness of green and transparent pheromone traps and the representation of non-target species were evaluated in three study areas of southern Moravia (Czech Republic) in 2019–2021 (24 traps per year). A total of 6 536 Grapholita molesta and 946 individuals of 17 non-target species were collected. Grapholita molesta was frequently captured in green delta traps with a Pherobank pheromone lure. Therefore, pheromone lures from different manufacturers have different efficacy and require the independent setting of harmfulness thresholds. An overview of non-target species recorded by other authors was compiled. Among the non-target species, morphologically similar species of Tortricidae dominated, causing considerable problems in the signalling of control measures of the target species.

The effect of parameter adjustment in sago palm classification-based convolutional neural network (CNN) modelOriginal Paper

Sri Murniani Angelina Letsoin, David Herák

Res. Agr. Eng., 2024, 70(3):123-133 | DOI: 10.17221/65/2023-RAE

In our study location, Merauke Regency, the easternmost city in Indonesia, the sago palm is associated with different types of ecosystems and other non-sago vegetation. During the harvesting season, the white flowers blossoming between the leaves on the tops of palm trees may be distinguished manually. Four classes were determined to address the visual inspections involving different parameters that were examined through the metric evaluation and then analysed statistically. The computed Kruskal-Wallis test found that the parameters vary in each network with a P-value of 0.00341, with at least one class being higher than the others, i.e., non-sago with a P-value of 0.044 with respect to precision, recall, and F1-score. Thus, the general linear model (GLM) was tested specifically in trained Network-15 and Network-17, which have similar parameters except for the batch size. It indicated the two networks' differences based on their prediction results, classes, and actual images. Accordingly, a combination of learning rate (Lr) and batch size improved the reliability of the training and classification task.

A study on the measurement of the reference range of the whole blood viscosity in Thoroughbred horsesOriginal Paper

Y Ha, K Yi, C Park, G Kim, D Choi, J Lee, N Kim

Vet Med - Czech, 2024, 69(11):381-388 | DOI: 10.17221/24/2024-VETMED


This study aimed to measure the whole blood viscosity (WBV) in racehorses using a new viscometer and establish reference values, as well as to investigate the correlation between the WBV and the haematological parameters and serum chemistry. WBV measurements were conducted on 51 Thoroughbred horses using a novel U-shaped scanning capillary-tube viscometer. The reference values for the WBV were determined at various shear rates ranging from 1 s–1 to 1 000 s–1. Correlation analyses were performed to examine the correlation between the WBV and the haematological and serum chemistry parameters. The findings provide valuable reference data for the WBV in Thoroughbred horses and enhance understanding of the relationship between the WBV and routine blood tests in equine health assessment.

Biochemical and antioxidant responses of common carp (Cyprinus carpio) exposed to sublethal concentrations of the antiepileptic and analgesic drug gabapentinOriginal Paper

J Blahova, P Mikula, P Marsalek, Z Svobodova

Vet Med - Czech, 2025, 70(1):20-29 | DOI: 10.17221/75/2024-VETMED

The present study aimed to evaluate the biochemical and antioxidant responses of common carp after exposure to the antiepileptic and analgesic drug gabapentin at concentrations of 0.1, 1, 10, and 100 μg/l for 4 weeks. The exposure to the highest two concentrations resulted in significant changes in plasma indices such as glucose (only group 100 μg/l), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lipase, creatine kinase (CK), amylase as well as butyrylcholinesterase (BChE). Similar trends were found in both groups exposed to the environmentally relevant concentrations (i.e., 0.1 and 1 μg/l). In addition, a significant increase in the ferric-reducing power of the plasma was noted in all treated groups. Numerous changes in antioxidant enzymes, superoxide dismutase, catalase, glutathione-S-transferase, as well as lipid peroxidation, were observed especially in the caudal kidney in the group exposed to 100 μg/l. Significant findings were also confirmed in the group exposed to an environmentally relevant concentration (1 μg/l), with a decrease in superoxide dismutase in the gill and an increased lipoperoxidation in the caudal kidney. Our research shows that subchronic exposure to gabapentin may pose a significant risk to non-target aquatic organisms, such as disruption of metabolic pathways or induction of oxidative stress, even at environmentally relevant concentrations.

The insertion of an ancestral gene in Nicotiana tabacum plants reduces free radicals during saline irrigationOriginal Paper

Claudia Marissa Calderón Torres, Victoria Abril Mancilla Galván, Miguel Murguía Romero

Czech J. Genet. Plant Breed., 2025, 61(1):43-49 | DOI: 10.17221/107/2024-CJGPB

Transgenic organisms modified with ancestral genes for nitrogen metabolism are rare. Previously, it was reported that genetically modified Nicotiana tabacum with the ARO4 gene of aromatic amino acid synthesis from the yeast Debaryomyces hansenii increases its growth during moderate salt stress. In this investigation, it was explored if the changes in the expression of the gene DhARO4 in Nicotiana tabacum, during saline irrigation, are related to the chlorophyll content and the total reactive oxygen species production. Seedlings of transgenic and wild type Nicotiana tabacum germinated in standard conditions were divided into two irrigation groups, with 100 mM of NaCl and with tap water; and, after 50 days, in the non-senescent adult leaves of the plants, the total chlorophyll a and b and the total chlorophyll content were determined by spectrophotometry and the reactive oxygen species production (•OH, 1O2, H2O2) was quantified by a 2',7'-dichlorodihydrofluorescein assay. The expression of the DhARO4 gene was verified with a salt shock of 100 mM of NaCl for 24 hours in the transgenic and wild type plants in the tap water irrigation group. The DhARO4 gene transcript increased (P < 0.05) in the transgenic plant; meanwhile, the average concentration of chlorophyll a increased (P < 0.05), and the average production of reactive oxygen species decreased (P < 0.05).

Comparison of three different methods for the determination of sulphur dioxide in fruit and vegetable productsOriginal Paper

Novel Kishor Bhujel, Eugene Okraku Asare, Tereza Podskalská, Kshitiz Pokhrel, Filip Beòo, Vojtìch Kru¾ík, Ale¹ Rajchl, Helena Èí¾ková

Czech J. Food Sci., 2025, 43(1):1-7 | DOI: 10.17221/113/2024-CJFS

Sulphite is a food additive used worldwide. Globally, for concentrations above 10 mg·kg–1, sulphite compounds must be labelled as sulphur dioxide (SO2) on the packaging due to their potential health risks. This study compares spectrophotometric (S), titration (T) (modified optimised Monier-Williams, OMW), and reflectoquant (R) methods for measuring sulphur dioxide in twenty fruit and vegetable products. The samples comprise sulphited, unsulphited, and naturally sulphur-containing products (Allium genus such as garlic and onion). The article discusses the strengths and weaknesses of the method used. Allium genus samples yielded false-positive results, especially in fresh garlic samples with average SO2 concentrations of 46, 1 152, and 40 mg·kg–1 obtained by titration, spectrophotometric, and reflectoquant methods, respectively, therefore, none of the methods is suitable for testing this type of vegetables or products containing a low proportion of them. For other types of samples, the methods showed acceptable working characteristics. Recovery tests showed 89.5, 82.0, and 75.2% recovery with 2.8, 3.9, and 13.2% repeatability and the limit of quantification of 1, 10, and 25 mg·kg–1 in the spectrophotometric, titration, and reflectoquant methods. The result highlights the importance of method selection based on sample characteristics and regulatory compliance.

Diameter growth performance of northern red oak (Quercus rubra L.) in northeastern HungaryOriginal Paper

Tamás Ábri, Zsolt Keserû, Veronika Honfy, Károly Rédei

J. For. Sci., 2025, 71(8):384-392 | DOI: 10.17221/39/2025-JFS

Northern red oak (Quercus rubra L.) is an important introduced tree species in Hungary, particularly in the Nyírség region. This study investigates its diameter growth (DBH) in six forest subcompartments, with stand ages ranging from 20 to 71 years. 5- and 25-year diameter increments were calculated. The distribution of diameter and diameter increment values was also determined. The results show significant growth across all sites. For example, quadratic mean diameter (QMD) increased from 16.4 cm to 30.4 cm over 25 years in the forest subcompartment Encsencs 12A, and from 20.3 cm to 38.4 cm in Ófehértó 2A. Five-year DBH increments ranged from 1.8 cm (± 0.8) in older stands to 4.2 cm (± 0.5) in younger or middle-aged stands. The findings confirm Q. rubra's capacity for steady diameter growth in northeastern Hungary and contribute valuable growth data for future yield modelling and forest management strategies.

Effects of praziquantel on early life stages of Grass carp, Ctenopharyngodon idellaOriginal Paper

J Velisek, A Strouhova, M Sandova, E Zuskova, P Dvorak, A Stara

Vet Med - Czech, 2025, 70(3):93-100 | DOI: 10.17221/85/2024-VETMED

This study aimed to assess the toxicity of the anthelmintic drug praziquantel in the early life stages of grass carp (Ctenopharyngodon idella). The toxicity was evaluated based on mortality, early ontogeny development, growth, oxidative stress biomarkers, antioxidant enzymes and histopathology. Praziquantel at all tested concentrations (1, 2, 4 and 6 mg/l) showed no significant adverse effects on the hatching of grass carp. Concentrations of 2, 4 and 6 mg/l praziquantel caused significantly (P < 0.01) higher mortality and slower growth compared with controls. Praziquantel at concentrations (4 and 6 mg/l) showed a significant (P < 0.01) delay of early ontogeny of grass carp. Concentration of 2 mg/l praziquantel caused significantly (P < 0.01) higher GST activity than controls. Among the groups, no histological changes were detected in tissues. For the early life of grass carp, praziquantel is safe at concentrations ≤1 mg/l.

Prevalence of subclinical mastitis, related risk factors, and antimicrobial susceptibility of bacteria isolated from the milk of dairy cows in KosovoOriginal Paper

N Mazreku, D Sylejmani, A Robaj

Vet Med - Czech, 2025, 70(4):117-123 | DOI: 10.17221/102/2024-VETMED


The purpose of this study was to determine the prevalence and risk factors associated with subclinical mastitis, as well as the antimicrobial susceptibility of bacteria isolated from the milk of dairy cows in the Republic of Kosovo. The study involved 242 lactating cows from 16 farms. Data were collected through questionnaire interviews, the California mastitis test (CMT), and microbiological analysis. For the detection and identification of bacteria, conventional methods and biochemical tests were used. The disc diffusion method was used to test the susceptibility of isolated bacteria. The prevalence of subclinical mastitis (SCM) at quarter and cow level was 22.3% and 10.5%, respectively. Factors including breed, age, parity, milk production, and lactation stage were significantly correlated with the occurrence of subclinical mastitis in cows (P < 0.05). Major bacterial isolates were Staphylococcus aureus (34%), coagulase-negative staphylococci (CNS, 21.3%), Escherichia coli (18.1%), Streptococcus uberis (14.9%), and Streptococcus agalactiae (4.3%). All bacterial isolates showed high susceptibility to gentamicin, tetracycline, amoxicillin/clavulanic acid, and sulfamethoxazole/trimethoprim and low susceptibility to penicillin and streptomycin.

Crystallinity of starch, food composition, and digestibility of starchReview

Ev¾en ©árka, Petra Smrèková, Marcela Sluková

Czech J. Food Sci., 2025, 43(2):90-104 | DOI: 10.17221/33/2025-CJFS

Starch granules in their native state are insoluble and semi-crystalline. There are three forms of starch/amylopectin in nature: A, B, and C: in cereals (A), tubers and high amylose starches (B), and some varieties of peas and beans (C). Crystallinity and rate of starch hydrolysis depend on the plant species and growing conditions. The changes during food preparation include gelatinisation, the formation of amylose-lipids and amylose-protein complexes, and resistant starch (RS) origin. They are accompanied by changes from crystal to amorphous form and vice versa. Starch in human food is mostly rapidly or slowly digestible. Rapidly digestible starch is formed by gelatinisation, cooking extrusion, breaking down of starch granules, or hydrolysis to maltodextrins. By definition, RS is not digested in the small intestine. This review addresses the influences of biochemical processes in the human body on starch digestibility. It is strongly influenced by the degree of chewing, the activity of α-amylase in the intestine, and transit time through the stomach and small intestine. Resistant starch and endogenous intestinal mucus support the growth of specialist microbes in the large intestine that produce a variety of short-chain fatty acids, causing the perception of satiety, lowering pH, and inhibiting pathogens in the colon.

Seed germination strategy as an indicator of suitability for restoration of species-rich meadowsOriginal Paper

Faisal Anggi Pradita, Maria Janicka

Plant Soil Environ., 2025, 71(5):353-362 | DOI: 10.17221/46/2025-PSE

Restoration of Arrhenatherion meadows is limited both by the lack of local seed availability in natural habitats for self-restoration purposes and the lack of information on the germination of target species in these meadows. Understanding germination strategies can optimise local seed use. This study aimed to define germination strategies for groups of species based on relevant six germination parameters: germination capacity (GC), fresh ungerminated seed (FUS), median germination time, germination velocity, germination synchrony and germination uniformity. The germination test of 23 meadow species was performed according to ISTA (International Seed Testing Association) rules. The hierarchical clustering method and PCA biplot divided the species into five groups. Based on the Kruskal-Wallis and Dunn’s test, the evaluation of six parameters in five groups showed that species such as Arrhenatherum elatius, Centaurea jacea, Plantago lanceolata, Tragopogon pratensis and Dianthus deltoides differed significantly in terms of higher GC, lower FUS and faster germination velocity than Lathyrus pratensis, Vicia angustifolia and Geranium pratense. Conversely, these three species had more synchronous germination than species such as Knautia arvensis and Briza media and expressed the shortest peak of germination period among other species. These six parameters potentially describe germination strategies across groups of species.

Enhancing the destructive egg quality assessment using the machine vision and feature extraction techniqueOriginal Paper

Ehsan Sheidaee, Pourya Bazyar

Res. Agr. Eng., 2025, 71(2):95-104 | DOI: 10.17221/86/2024-RAE

The rapid growth of the food industry necessitates rigorous quality control, particularly in egg production. This study explores advanced methodologies for egg quality assessment by integrating the Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), and k-Nearest Neighbour (KNN) with machine vision techniques. While traditional destructive methods like measuring the Haugh unit (HU) offer direct insights, but render eggs unusable, non-destructive techniques, such as imaging and spectroscopy, allow continuous quality monitoring. Over a 20-day period, egg samples were evaluated using a digital camera to capture key parameters like the albumen and yolk heights. The study’s image processing involved noise reduction, feature extraction, and calibration. The PCA captured 90.18% of the data variability, while LDA achieved 100% classification accuracy, and KNN demonstrated 80% accuracy. These findings underscore the effectiveness of combining machine vision with statistical methods to enhance the egg grading accuracy, contributing to consumer safety and industry standards.

Effects of flour, starch and pea (Pisum sativum L.) protein as fat substitutes during storage of pork sausagesOriginal Paper

Gema Morales-Olán, María Antonieta Ríos-Corripio, Marlon Rojas-López, Joel Velasco-Velasco, Aleida Selene Hernández-Cázares

Czech J. Food Sci., 2025, 43(3):194-204 | DOI: 10.17221/211/2024-CJFS

Efforts are being made to replace the fat in meat products such as sausages with vegetable compounds to generate healthier foods. In this work, the effects of including flour, starch, and proteins isolated from pea seeds as partial fat substitutes in pork sausages was evaluated by studying the proximate composition, energy content, total cholesterol, lipid oxidation, and physicochemical, textural, and structural properties during refrigerated storage. The results showed significant differences in the composition of the sausages. Low-fat flour (LFF), starch (LFS), and pea protein (LFP) sausages had approximately 18% lower energy content than high-fat (HF) sausages. Cholesterol content was not significantly different in the treatments. Cooking yield, pH, and water activity were not affected by the inclusion of the replacements. LFF sausages had the highest purge losses and LFP sausages the lowest. The addition of pea starch improved the luminosity of the sausages, but the addition of pea protein resulted in darker sausages. After 12 days of storage, no differences were found between the hardness of LFP and HF sausages. The replacements did not affect lipid oxidation. The results suggest that replacing fat with pea seed components may be an alternative to producing low-fat sausages with health benefits.

Forest as a biological asset – An accounting and tax perspective in the Czech RepublicOriginal Paper

Petra Hlaváèková, Jitka Fialová, Jiøí Schneider

J. For. Sci., 2025, 71(7):336-346 | DOI: 10.17221/31/2025-JFS

This paper analyses the accounting and tax treatment of forests as biological assets in the Czech Republic under Act No. 563/1991 Coll., on Accounting, and its implementing regulations. It compares national approaches with the International Financial Reporting Standards (IFRS), focusing on the International Accounting Standard IAS 41 – Agriculture. A comparative-analytical method was used to identify key differences in classification, valuation, and reporting practices. Czech legislation values forests at historical cost and classifies them as non-depreciable land, whereas IFRS requires fair value measurement, capturing biological transformation and the economic potential of forest ecosystems. The findings show that while the Czech framework ensures legal clarity and tax alignment, it lacks integration with environmental accounting frameworks such as the United Nations System of Integrated Environmental Economic Accounts (SEEA) and does not reflect ecosystem services or ESG (environmental, social, governance) reporting needs. In contrast, IFRS provides a dynamic representation of forest assets, supporting transparency and comparability in international contexts. The study contributes to the ongoing harmonisation debate by offering recommendations to improve the alignment of Czech accounting with international standards, thus supporting sustainable forest management and enhancing investment credibility. Its originality lies in combining legislative analysis with international practice review, highlighting the gap between ecological value and financial reporting in forestry.

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