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Results 661 to 690 of 4099:

Conduction block and thin and hypokinetic myocardial segments in feline hypertrophic cardiomyopathyCase Report

Y Sung, J Park, Y Chae, T Yun, BT Kang, H Kim

Vet Med - Czech, 2025, 70(8):302-306 | DOI: 10.17221/14/2025-VETMED

A 12-year-old castrated male domestic shorthair cat was referred for respiratory distress. Physical examination revealed a systolic heart murmur at the left apex and crackles in all lung fields. Thoracic radiography showed Valentine-shaped cardiomegaly, pulmonary oedema, and pleural effusion. Echocardiography revealed focal thickening of the interventricular septum [11.01 mm; reference interval (RI) = 3.00–5.20 mm] and left ventricular posterior wall (7.41 mm; RI = 3.00–5.10 mm) during diastole. In the apex region, the free wall was focally thinned to approximately 1.6 mm with hypokinetic myocardial movement, indicating thin and hypokinetic myocardial segments. Additionally, decreased left atrial fractional shortening (12.5%; RI = 23.9–34.9%) and an increased left atrial-to-aortic ratio (2.87; RI = 0.88–1.43) were observed, along with spontaneous echocardiographic contrast in the left atrium, indicating increased thrombotic risk. The electrocardiogram showed a left axis deviation with small R waves and deep S waves in lead II, which is consistent with a left anterior fascicular block caused by delayed conduction in the left anterior fascicle. This case report describes the coexistence of a left anterior fascicular block and thin, hypokinetic myocardial segments in feline hypertrophic cardiomyopathy, suggesting a possible pathophysiological link.

Development of sessile oak [Quercus petraea (Matt.) Liebl.] seed coating material against rodents and evaluation of its performance on seed germination and emergenceOriginal Paper

Mesut Tandoğan, Mehmet Özdemir, Vedat Aslan, Ahmet F. Hacıyusufoğlu, Ali Ayhan Kul, Emine ªen, Kaan Polatoğlu, Duygu Sultan Oran

J. For. Sci., 2025, 71(9):417-425 | DOI: 10.17221/42/2025-JFS

Protecting sessile oak [Quercus petraea (Matt.) Liebl.] seeds from rodents is crucial to ensure successful germination and emergence in activities such as artificial regeneration, afforestation, and seedling production. This study examined the effects of 12 natural or nature-identical substances, believed to have repellent properties, on the germination, emergence, and survival of sessile oak acorns under both laboratory and field conditions. Acorns were coated using a diatomaceous clay-based pellet system, and a Y-maze experiment was conducted to evaluate rodent behaviour. As a result of the research, among the tested substances, Ferulago confusa and Foeniculum vulgare were recommended as rodent repellents due to their success in laboratory and field trials. Diesel fuel + hair, a conventional repellent, showed poor performance and is not recommended. This study underscores the potential efficacy of natural or nature-identical coatings for protecting seeds from pests in forestry applications.

Impact of the adoption of chemical inputs on crop yield downside riskOriginal Paper

Olha Aleksandrova, ©tefan Bojnec

Agric. Econ. - Czech, 2025, 71(10):527-536 | DOI: 10.17221/298/2024-AGRICECON

The study aims to analyse the impact of application of chemical inputs like fertilisers or crop protection products on farm crop yield productivity in Estonian and Slovenian agriculture. We combined the propensity score matching (PSM) method with an inverse probability weighted regression (IPWRA) model to derive treatment effects of the adoption of these critical inputs using Farm Accountancy Data Network data. Results exhibit consistency across estimation techniques. Estimates of both IPWRA and PSM models showed that adoption of at least one of the chemical inputs decreases volatility of crop yield output and downside risk. The results are more robust for Estonian than for Slovenian farms suggesting on possible impacts of other exogenous factors such as climate change on mitigating the crop yield downside risk.

Coagulation profile in bitches with pyometra: Standard tests and thromboelastographyOriginal Paper

A Ramesova, K Machackova, I Vanova, M Lacinova, A Bartoskova, R Novotny, R Vitasek, K Rehakova, J Doubek

Vet Med - Czech, 2025, 70(11):408-416 | DOI: 10.17221/16/2025-VETMED

This study aimed to determine coagulation changes in bitches with pyometra based on a series of coagulation tests and thromboelastography (TEG), and to assess the incidence of disseminated intravascular coagulation (DIC). Eighteen bitches with pyometra and thirty-four control bitches were examined. Haematological, biochemical, and following haemostasis parameters were measured, including: prothrombin time (PT), activated partial thromboplastin time (aPTT), antithrombin activity (AT), and levels of fibrinogen (FBG), d-dimers (DD), tissue factor (TF), plasminogen (PLG), tissue plasminogen activator inhibitor 1 (TPAI-1), and thromboelastography. DIC was considered present if three or more of these parameters were abnormal: platelet count (<153 × 109/l), PT (>8.1 s), aPTT (>25.5 s), FBG (<0.6 g/l), DD (>0.2 mg/l), and AT (<107%). Significant differences were found in the PT, aPTT, FBG, DD, TPAI-1, clotting time, α-angle, and maximal amplitude. According to our scoring system, two patients were DIC positive. The study found alterations in several coagulation tests and hypercoagulable TEG tracings in bitches with pyometra, which point to excessive activation of coagulation, delayed fibrinolysis, and the presence of DIC. No patient bled abnormally, which may suggest that DIC is not overt in the majority of pyometra patients.

Occurrence of herbicides and their transformation products in sewage sludge: a reviewReview

Madhusha Mihirani Subasinghe, Filip Mercl, Sreynet Nang, Altyn Taisheva, Pavel Tlusto¹

Plant Soil Environ., 2025, 71(12):849-859 | DOI: 10.17221/485/2025-PSE

The beneficial reuse of sewage sludge in agricultural soils is limited by the accumulation of micropollutants of emerging concern, which may pose significant environmental and human health risks. This review summarises recent advances in understanding the occurrence, persistence, and fate of herbicides and their transformation products in sewage sludge. Data from various geographic regions are discussed, with a focus on implications for the safe reuse of biosolids in agriculture. Most available studies have been conducted in European Union countries, where land application of biosolids is a common practice. Twelve groups of herbicides and their transformation products have been identified in sewage sludge, including glyphosate and aminomethylphosphonic acid (AMPA), phenylureas, phenoxy acids, chloroacetamides, triazines and their metabolites, triazinones, phenylcarbamates, isoxazolidinones, benzoic acids, dinitroanilines, benzofurans, phenyl ethers, and other herbicides. Among these, triazines and their metabolites were the most frequently detected, with concentration ranges of 0.01–277 ng/g and not detected (n.d.)–237 ng/g, respectively. Glyphosate and AMPA were found at particularly high concentrations (n.d.–35 000 ng/g). Phenylurea herbicides (e.g., diuron and isoproturon) were detected in a limited number of studies, with concentrations ranging from not detected to 102 ng/g. Substantial concentrations of phenoxy herbicides (2,4-d, 2,4-db, and 2,4,5-t) were also reported in sewage sludge, ranging from 50.5–864 ng/g. The available scientific literature on the occurrence of herbicides in sewage sludge focuses mainly on older, often already banned compounds, while data on currently approved herbicides remain scarce. This review highlights the need for more comprehensive global assessments of herbicides and their transformation products in sewage sludge to ensure the safe agricultural use of biosolids and minimise risks to plants and other organisms. The current lack of systematic monitoring and documentation represents a critical knowledge gap in evaluating environmental exposure and associated risks.

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A reviewReview

S Kumar, MS Chaves, AFB da Silva, WG Vale, STR Filho, JC Ferreira-Silva, LM Melo, VJF Freita

Vet Med - Czech, 2023, 68(2):45-56 | DOI: 10.17221/48/2022-VETMED


Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes.

Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumesOriginal Paper

Melike Bakır, ªehriban Demir, Cebrail Yıldırım, Abdullah Kahraman

Czech J. Genet. Plant Breed., 2024, 60(1):42-49 | DOI: 10.17221/57/2023-CJGPB

Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition, we tested the transferability of 86 SSR markers, including the 33 newly developed ones, to other legume species. Transferability rates of lentil SSR markers to other species varied between 13.76% (Vicia sativa L.) and 2.58% (Phaseolus vulgaris L. and Trifolium pratense L.). These new SSR markers could be used in further molecular breeding, population genetics, and genetic mapping studies in lentils and other legume species.

Approximation of the soil particle-size distribution curve using a NURBS curveOriginal Paper

Adéla Marie Marhoul, Tomá¹ Herza, Josef Kozák, Jaroslava Jankù, Jan Jehlièka, Lubo¹ Borùvka, Karel Nìmeèek, Miroslav Jetmar, Petr Polák

Soil & Water Res., 2025, 20(1):16-31 | DOI: 10.17221/139/2024-SWR


Soil particle-size distribution or soil texture presents one of the most important physical properties. There are various systems of the classification systems for soil particle-size fractions with different boundaries. Our effort was concentrated on the mathematical approach to evaluate the existing data and convert it to the form of a reconstructed cumulative particle-size curve which will allow reading concentration of any desired particle size. Non-Uniform Rational B-Splines (NURBS) curves therefore represent a generalization of B-splines and Bézier curves by extending the definition by an element of rationality, which is represented by the weights of the control points, and a nodal vector of parametrization, which represents the element of uniformity. The NURBS curve was used for smooth (depending on the degree of the curve used) and as tight as possible approximation of the arranged control points, the connecting lines of which forms a convex envelope for its individual parts. The NURBS approximation curve is therefore determined by the ordered control points and their connecting lines, the weights of these points, the degree of the curve and the nodal vector of parametrization. However, the construction of the approximation curve is primarily dependent on a limited number of points of the experimentally determined particle-size distribution curves, and for curves with significant breaks in the course, one must consider either a lower accuracy of the approximation or the necessity of “improving” the approximation using the weights of individual points, inserting additional points or working with a nodal vector of parametrization. For basic approximation, the PUGIS system (Czech soil information system) offers automatic approximation using all variants mentioned in the article as well as the possibility of individual changes in the weights of control points, in their number and position, and in the nodal vector of parametrization.

Accidental salinomycin intoxication in European fallow deer (Dama dama L.)Case Report

M Svoboda, O Huml, P Chomat, A Honzlova, J Illek, Z Svobodova, L Hofmannova, H Modra

Vet Med - Czech, 2024, 69(1):18-29 | DOI: 10.17221/100/2023-VETMED


Salinomycin, belonging to ionophore antibiotics, has been used as a feed additive for poultry for its coccidiostatic effect. Poisoning by ionophore antibiotics has been reported in cattle and other sensitive animals due to the replacement of medicated feed and/or accidental overdoses. The aim of this paper is to report the toxicity of salinomycin for fallow deer and to describe the different levels of sensitivity of cervids to this substance. In the presented case study, a medicated feed containing ivermectin used for deworming red deer and fallow deer was accidentally contaminated with sodium salinomycinate in a concentration of 252.6 mg/kg. The contaminated feed was consumed by the animals over a period of four days. The mortality of fallow deer within 12 days was 58%. No mortality was recorded in the red deer. In the affected animals, clinical signs associated with acute and congestive heart failure were observed. The biochemical examination indicated prerenal azotaemia caused by circulatory insufficiency and ion imbalance. The histological examination revealed pronounced focal acute cardiomyopathy and massive subacute myopathy in the skeletal muscles.

Controlling Phytophthora blight of pepper in Guizhou Province of China using Stellera chamaejasme extracts and synthetic chemical fungicidesOriginal Paper

Jiling Dang, Xingren Shi, Yuquan Lin, Mark L. Gleason, Jia Feng

Plant Protect. Sci., 2024, 60(1):53-64 | DOI: 10.17221/84/2023-PPS

Phytophthora blight of pepper, caused by Phytophthora capsici Leonian, is a destructive disease in pepper production. Extracts of the plant species Stellera chamaejasme Linn, previously known in China as a source of herbal medicine, were also used in former years as a toxin against aphids, mites, and plant-pathogenic fungi. Extracts of S. chamaejasme (SC) and seven commercial fungicides were investigated for their inhibition of mycelial growth and germination of cysts of the pathogen in vitro. The SC alone, infinito (fluopicolide + propamocab + hydrochloride), fluazinam, dimethomorph, and their mixtures with SC strongly inhibited both mycelial growth and germination of cysts of P. capsici. Tests were conducted in a commercial field in Fenggang County, Zunyi City, Guizhou Province, China, in 2019, 2020, 2021, and 2022 to validate these results for growers. A soil application (7 days before transplanting) and three foliar sprays at 7, 10, or 15 days were tested. One soil application of SC followed by three successive foliar sprays of infinito, fluazinam, and dimethomorph at 7- or 10-day intervals, as well as tank-mixed applications of these synthetic fungicides with SC at 15-day intervals, limited Phytophthora blight incidence to < 10% on the non-treated control treatment. Disease incidence was limited to < 3% to < 5% when alternating SC, SC + infinito, SC + fluazinam, and SC + dimethomorph three times at 7-, 10- or 15-day intervals, plus one soil application of SC. When treatments were applied from early April to May, disease incidence was < 10% in the non-treated control during June and July. The optimal concentration of SC was determined to be 0.25 g/L, which effectively controlled Phytophthora blight and protected marketable yield. SC also significantly (P < 0.05) outperformed treatments in a nearby commercial pepper field that relied on applications of a single synthetic chemical fungicide. The findings of this study provide a foundation for guiding growers to implement an efficient and environmentally safe spray program against Phytophthora blight of pepper in Guizhou Province.

Understanding the cross-cultural chicken consumers' behaviorOriginal Paper

Fallah Samuel Kassoh, Baichen Jiang, Achara Boonkong, Hui Li, Abdelrahman Ali, Tanapon Srisukwatanachai

Agric. Econ. - Czech, 2024, 70(2):73-90 | DOI: 10.17221/331/2023-AGRICECON


Concerns for human health and food safety are gaining momentum in emerging and developing nations as well as in food research due to the reputations that products have established over time. Understanding cross-cultural consumers’ purchasing behaviour for chicken meat (CM) could provide valuable insights into the global food sector since it is the most consumed meat worldwide. This study examines the effect of buying features (BF), culture factors, behavioural attitudes (BA), subjective norms (SN) and perceived behavioural control (BC) on CM actual purchase behaviour (APB) in China and Sierra Leone. Primary data were sourced from 588 Chinese and 410 Sierra Leonean participants. We adopt structural equation modelling (SEM) to verify the model and test the associations between the variables. The SEM results confirm that BF, BA, SN and BC stimulate customers’ intent and actual purchase of CM in both nations. The findings indicate that cultural factors (CF) significantly moderates the association between the independent constructs (BF, BA, SN and BC) and APB in Sierra Leonean markets. This study provides significant insights for chicken industry stakeholders that can help them increase CM sales along the supply chain via a marketing strategy focused on cultural and psychological factors.

Can income growth and environmental improvements go hand in hand? An empirical study of Chinese agricultureOriginal Paper

Chenchen Su, Jinchuan Shen, Fei Wang

Agric. Econ. - Czech, 2024, 70(7):321-333 | DOI: 10.17221/438/2023-AGRICECON

Advancing agricultural carbon emission efficiency and farmers’ income simultaneously is crucial for the sustainable progress of agriculture. Our study centered on 31 provinces and cities in China and investigated regional variances and the dynamic evolution aspects of coordinated development in farmers’ income and agricultural carbon emission efficiency, utilising panel data from 2005 to 2021. The analysis revealed the following trends: Firstly, China’s overall agricultural carbon emission efficiency was steadily increasing, mainly because of technological advancements. Secondly, the correlation between farmers’ income and agricultural carbon emission efficiency in China had notably improved from 0.318 to 0.775. Throughout the observation period, disparities in the levels of coordinated development were observed in various provinces with a diminishing fluctuation, indicating regional disparities as the primary instigator. Thirdly, the level of coordinated development highlighted positive clustering attributes, demonstrating an ‘east high, west low’ pattern; the evolution of coordinated development levels exhibited stability in maintaining the current status. This study holds significant value for developing countries in enhancing farmers’ income and agricultural carbon emission efficiency in a coordinated manner.

Enriching wheat flour with grape pomace powder impacts a snack's chemical, nutritional, and sensory characteristicsOriginal Paper

Amal Hassan Alshawi

Czech J. Food Sci., 2024, 42(4):243-250 | DOI: 10.17221/103/2024-CJFS


Because grape pomace powder (GPP) contains abundant phenolic chemicals and fibres, GPPs can serve as a filler in developing novel food products. This study examined how the GPP amounts affected a composite flour's physicochemical properties and bakery snacks' chemical, technical, and sensory properties. The experimental procedure involved replacing wheat flour (WF) with GPP at 5–20% while maintaining 100% WF as the control. The addition of GPP resulted in a significant decrease in the oil absorption capacity (P ≤ 0.05), while the rehydration index and water absorption capacity increased (P ≤ 0.05). The peak length (5.44–5.90 min), pasting temperature (70.20–80.92 °C), peak viscosity (124.72–172.80 RVU; RVU – relative value unit), trough viscosity (60.76–82.04 RVU), breakdown viscosity (69.56–93.74 RVU), final viscosity (162.70–222.30 RVU), and setback viscosity were measured. The addition of GPP to the composite flour and snacks decreased the lightness (L*) and increased the redness (a*) and yellowness (b*). The items' higher dietary fibre (DF) allowed them to claim 'high fibre content' when the maximum GPP was added. The GPP also increased the snacks' total solids, protein, ash, fibre, total phenolics content (TPC), and antioxidant capacity. The sensory acceptability of the snacks made with 5–10% GPP instead of WF was higher.

Characteristics of the workplace of harvester operators in PolandOriginal Paper

Dominika Gaj-Gielarowiec, Karol Gielarowiec, Grzegorz Szewczyk, Pawe³ Tylek

J. For. Sci., 2024, 70(10):501-511 | DOI: 10.17221/49/2024-JFS


The objective of the presented research was to create an average image of a harvester operator in Poland and to identify factors influencing the degree of mental workload. We used a survey of environmental assessments of workload. The creation of an electronic survey and the support of a company that indirectly employs operators (State Forests) made it possible to reach a large group of surveyed people. The respondents indicated the occurrence of ailments typical of sedentary work at the researched workstation. These included mainly monotony, back pain and numbness in the upper limbs. Stress at work was felt by the majority of respondents; hence the analyses of mental stress, which also translates into other diseases, should indicate key responses to questions regarding comprehensive ergonomic assessments at the researched workstation.

Successful pregnancy of a Maine Coon queen despite feline mammary fibroadenomatous hyperplasia recurrence after treatment with aglepristoneCase Report

P Socha, P Mossakowski

Vet Med - Czech, 2025, 70(1):30-34 | DOI: 10.17221/51/2024-VETMED


One of the disorders of the mammary gland in the queen is feline mammary fibroadenomatous hyperplasia (FMFH), caused by an increasing concentration of progesterone (P4) and some other local growth factors. It occurs mostly during puberty after the heat characterised by spontaneous or provoked ovulation, as a result of exogenous progesterone intake and sometimes during pregnancy. To diagnose a 14-month-old intact Maine Coon queen with extensive mammary gland hyperplasia, a clinical examination, analyses of the progesterone (P4) concentrations and ultrasound examination were performed. Feline mammary fibroadenomatous hyperplasia associated with a high P4 concentration after spontaneous ovulation was confirmed. After 24 days of therapy with a progesterone antagonist, aglepristone, the symptoms of FMFH resolved. After the next eight weeks, the queen was mated after the owner’s decision. In the third week of pregnancy, a relapse was detected (mammary gland enlargement, pain, discomfort). At the same time, no abnormalities in the uterus or embryos were detected via ultrasound. The P4 concentrations were under regular control. For the next two weeks, only conservative treatment with NSAIDs was used. The queen spontaneously delivered six kittens without any difficulties or perinatal complications 67 days after the first mating. The cat previously treated with aglepristone for FMFH was successfully bred, but FMFH symptoms returned when progesterone concentrations increased during pregnancy.

Optimising sunflower yields: insights from meta-analysis on fertilisation impact and planting strategies for enhanced crop productivity in ChinaOriginal Paper

Shun Li, Zongqing Liu

Plant Soil Environ., 2025, 71(1):48-57 | DOI: 10.17221/303/2024-PSE

Sunflower serves as a valuable rotational crop, suitable for snack processing or sunflower seed oil extraction, proving to be a lucrative cash crop. To address sunflower yield uncertainties, this study employs meta-analysis to examine the impact of fertilisation. Utilising 41 studies and 392 pairs of observations based on four criteria, we found an overall 27% increase in sunflower yield with fertiliser application. Nitrogen (N), phosphorus (P), and potassium (K) individually applied raised yield by 23.37, 20.92, and 11.63%, respectively. Combined fertilisers (NP, NK, NP, and NPK) enhanced yield by 29.69, 28.40, 17.35, and 41.91%, respectively. Sunflower type minimally affects yield, while planting density significantly influences it. Combining local soil conditions and environmental factors with appropriate planting densities ensures maximum sunflower yield, fostering economic benefits for farmers. This study holds constructive implications for sunflower cultivation in China, contributing to increased yield.

The role of seed transmission in the spread of cereal viruses: Global challenges and prevalent threats in UkraineReview

Halyna Snihur, Tetiana Shevchenko, Oleksiy Shevchenko, Anhelina Kyrychenko

Plant Protect. Sci., 2025, 61(3):201-221 | DOI: 10.17221/51/2025-PPS

The transmission of plant viruses through seed plays a fundamental role in virus spread, persistence, and survival, particularly in economically important crops. Besides its considerable ecological significance, seed transmission influences plant and virus evolution. Virus contamination of the seed also has critical epidemiological implications, especially when combined with subsequent or additional insect vector spread. Plants grown from contaminated seeds serve as primary viral inoculum sources, facilitating the introduction of viruses into new regions and triggering disease outbreaks with substantial economic losses for growers. Changes in environmental conditions increasingly influence plant virus epidemiology by affecting vector populations, host susceptibility, and transmission dynamics, thus increasing virus transmission risks in cereal crops. This review explores the mechanisms of seed transmission and its consequences, with a focus on key cereal viruses in Ukraine: barley stripe mosaic virus, wheat streak mosaic virus, High Plains wheat mosaic virus, sugarcane mosaic virus, and maize dwarf mosaic virus. Hereby, the biological properties of these viruses, the risks posed by seed transmission, and the economic impact on crop production are discussed. Given the widespread distribution of these pathogens, presented data will also be valuable for other cereal-growing regions, particularly those bordering Ukraine and engaged in seed import/export. This review underscores the global need to manage seed-transmitted viruses to safeguard cereal crop productivity and food security. Future research should focus on developing resistant cultivars and advanced diagnostics to control their spread.

Prevalence and risk factors of Cryptosporidium spp. and Giardia infestation in cattle and in broiler chickens in AlgeriaOriginal Paper

R Baazizi, M Taibi, N Senouci, D Baroudi, S Khodja, R Belala, D Khelef, N Mimoune

Vet Med - Czech, 2025, 70(2):68-76 | DOI: 10.17221/82/2024-VETMED

Cryptosporidiosis and giardiasis are zoonotic protozoan diseases with significant public health and economic concerns. In Algeria, epidemiological data on these parasites in livestock are limited. This study aimed to estimate the prevalence of Cryptosporidium spp. and Giardia in dairy calves and broiler chickens and to identify the associated risk factors. A microscopic analysis of 200 faecal samples revealed a Cryptosporidium spp. prevalence of 56% in calves and 60% in broiler chickens, while the Giardia prevalence was 8% in calves and absent in chickens. In cattle, the data showed that age constituted a potential risk factor for both parasites (P < 0.000 1 for Cryptosporidium; P < 0.000 5 for Giardia). Interestingly, the risk of a Cryptosporidium infection decreased with age, while the Giardia infestation risk increased. The sex was not a significant factor for Cryptosporidium (P = 0.115 0), but was impactful for Giardia (P < 0.000 1), with males at higher risk. These results highlighted the distinct epidemiological characteristics of Cryptosporidium and Giardia infestations in Algerian livestock. The contrasting age-related risks and sex-specific susceptibility to Giardia underline the need for targeted, age and sex prevention strategies. This study provides valuable data to inform public health policies and to improve livestock management practices in Algeria, contributing to the wider understanding of these zoonotic parasites in North African agricultural farming.

Biocontamination in the dairy industry: The effect of raw milk conditioning film on the adhesion of Escherichia coliOriginal Paper

Zakariyae El hafa, Ikhlas Hani Chennoufi, Hafida Zahir, Mostafa El louali, Hassan Latrache

Czech J. Food Sci., 2025, 43(2):112-117 | DOI: 10.17221/91/2024-CJFS

Conditioning films on surfaces employed in the dairy industry serve as the precursors to the formation of pathogenic biofilms that impact product quality and consumer safety. Conditioning films have been studied from several aspects. However, there has been no study that evaluated the effect of raw milk conditioning film on the adhesion of Escherichia coli. This study investigated the adhesion of E. coli on glass and stainless-steel surfaces conditioned with raw milk and explored the surface properties potentially influencing this adhesion using the contact angle method. The results showed that after treating surfaces with raw milk, the adhesion of the bacteria on stainless steel and glass was significantly altered. Adhesion increased significantly on stainless steel (from 0.55 log10 to 2.8 log10) but it decreased on glass (from 1.56 log10 to 0.8 log10). Significant alterations were observed in the physicochemical properties of the surfaces. Glass was initially relatively hydrophilic (46.33°), while stainless steel was relatively hydrophobic (82.5°). After treatment, the glass became relatively more hydrophobic (74.6°), and stainless steel became relatively more hydrophilic (69.4°). The electron donor/acceptor components of glass decreased after the treatment, while these components increased for stainless steel. The significant changes in adhesion were hypothesized to be due to the modification of surface properties by the raw milk.

The potential of probiotics derived from functional foods for skin healthReview

Eunhye Son

Czech J. Food Sci., 2025, 43(4):235-245 | DOI: 10.17221/35/2025-CJFS

Probiotics, widely recognised for their benefits in gut health, have gained increasing attention for their comprehensive role in skin health maintenance and improvement. This review explores the multifaceted impact of food-derived probiotics on various aspects of skin health, including anti-aging, inflammation regulation, barrier function enhancement, and hydration. Probiotics exert protective effects against oxidative stress and inflammatory responses through modulation of the gut-skin axis, enhancement of the skin microbiome balance, and immunomodulatory properties. Additionally, functional foods enriched with probiotics have demonstrated efficacy in promoting skin hydration, elasticity, collagen production, and overall resilience. By synthesising recent research findings, this review highlights the potential of probiotic-enriched foods as a natural approach to supporting comprehensive skin health and addressing age-related skin changes. The implications for functional food development and dietary interventions are also discussed, providing insights into future research directions in probiotic-based holistic skin care strategies.

Balancing quality and safety: Optimising drying and sodium metabisulphite use in low-fat desiccated coconutOriginal Paper

Indriati Cahyadewi, Waqif Agusta, Rumpoko Wicaksono, Tantry Eko Putri Mariastuty, Lusiana Kresnawati Hartono, Herdiarti Destika Hermansyah, Farah Nuranjani

Res. Agr. Eng., 2025, 71(3):130-142 | DOI: 10.17221/3/2025-RAE


Indonesia, as a leading coconut producer, generates a substantial amount of coconut pulp from coconut milk extraction, which can be further processed into low-fat desiccated coconut. However, the drying process induces browning, reducing its whiteness and overall quality. Sodium metabisulphite is commonly used to prevent browning, but excessive use raises health concerns. In this study, the optimisation of the drying temperature and sodium metabisulphite concentration was conducted to minimise the browning while maintaining the quality of low-fat desiccated coconut. Using Response Surface Methodology (RSM) with a Central Composite Design (CCD), responses such as the browning index (BI), whiteness index (WI), moisture content, yield, free fatty acids (FFAs), ash, fat, protein, total phenolic content (TPC), and crude fibre were examined. The results showed that both the drying temperature and sodium metabisulphite concentration significantly influenced the physicochemical properties. The optimal conditions were identified at 62.505 °C and 380.059 ppm sodium metabisulphite, resulting in a whiteness index of 87.219, browning index of 5.1025, yield of 43.125%, moisture content of 2.3%, and free fatty acid content of 4.45%. These findings highlight an effective strategy for reducing the additive dependency while maintaining the physicochemical quality of low-fat desiccated coconut.

Unveiling green perception effect: How live streaming scenes and green information influence purchase intention of prepared dishesOriginal Paper

Shuo Hao, Pei Li, Nannan Zhang, Yang Du, Rui Liang, Ziyang Liu

Agric. Econ. - Czech, 2025, 71(10):537-549 | DOI: 10.17221/247/2024-AGRICECON

To promote the sustainable consumption of prepared dishes, this study examines how green attribute information (GAI) and live streaming scenes experience (LSSE) influence consumers' green perceptions (GP) – including perceived green value (PGV) and perceived green trust (PGT) – and subsequently affect their purchase intention (PI). A green perception model was developed and tested using a mixed-method approach. Word cloud analysis was conducted on consumer comments collected from e-commerce platforms to extract key descriptors and concerns, which informed the design of a structured questionnaire. A total of 291 valid responses were analysed using partial least squares structural equation modelling (PLS-SEM). Results show that: (iGAI does not directly impact PI, indicating that GP is a necessary mediator; (iiGAI significantly influences PGV, but not PGT; (iiiGP fully mediates the GAIPI relationship, although PGV has a negligible direct effect on PGT; (ivLSSE significantly enhances both GP and PI; (vGP partially mediates the LSSEPI relationship. These findings highlight the dual role of LSSE in facilitating information adoption and emotional engagement, and suggest that enhancing LSSE design and enriching GAI are effective strategies to increase consumers' green perception and purchase intention towards prepared dishes.

Detection of heat-stressed chickens in poultry house based on deep network and optical flow vectors in the Fourier domainOriginal Paper

Ngo Quoc Viet, Thai Yen

Res. Agr. Eng., 2025, 71(4):189-199 | DOI: 10.17221/46/2025-RAE

The productivity and quality of the entire flock are negatively impacted by heat stress in chickens, which can have major repercussions, particularly in crowded farming settings where diseases are easy to spread and hard to control. This study uses deep networks and optical flow to identify heat stress in chickens. The technique focuses on identifying obvious signs of heat stress, such as panting and open-mouth breathing in chickens. There are two phases to the suggested approach: (1) using a deep network to detect open-mouth breathing in chickens; (2) using the Gunnar Farnebäck algorithm to compute the optical flow vectors of the wattle, the breathing frequency is estimated in the Fourier domain for the detection of panting chickens. The proposed method was tested on the obtained dataset and demonstrated its ability to recognise heat-stressed chickens in crowded conditions, achieving an overall performance metric of 0.90 by integrating the results of both phases. The two-phase approach, which incorporates the open-mouth breathing behaviour and panting frequency, improves the efficiency and assures robust, reliable heat stress detection.

A comparative applied analysis of six robotic-assisted weeding systems in sugar beetsOriginal Paper

Sonja I. Kimmel, Matthias Schumacher, Michael Spaeth, Markus Sökefeld, Oyebanji O. Alagbo, Alicia Allmendinger, Dionisio Andujar, Therese W. Berge (ORCID: 0000-0002-8780-

Plant Soil Environ., 2025, 71(11):782-792 | DOI: 10.17221/335/2025-PSE

Effective weed management is crucial in the critical period of sugar beet production, but often lacks sustainability and environmental protection. Recent advancements in sensor-based weed control systems have rendered the latter a realistic prospect, which demands detailed analyses, especially under suboptimal field conditions. The present study analysed six robotic-assisted weed control systems (RAWS) in three experiments on sugar beets in 2024, conducted under dry soil and high weed pressure. The experiments included sensor-based inter-row and intra-row hoeing, spot- and band-spraying and were compared to a broadcast herbicide treatment and an untreated control. Weed control efficacy (WCE) in the intra- and inter-row areas, as well as weed species composition and crop plant damage, were assessed after treatment. The data show that intra-row WCE of two hoeing robots (Farming GT® and Robovator®) equipped with selective intra-row blades achieved up to 80%, which was higher than the broadcast herbicide control with 67% WCE. In the inter-row area, Farming GT® robotic hoeing and ARA® spot-spraying resulted in more than 90% WCE, which was equal to the broadcast herbicide application. Weed species composition was not affected by the different RAWS. Crop plants were affected by all hoeing treatments with maximum non-lethal burial rates of 33%. The highest lethal uprooting of crop plants occurred after Farming GT® robotic hoeing, at 5.5% overall. The results demonstrate the great potential of robotic weeding to replace broadcast herbicide applications.

Enhancing rheological properties of dough and quality of potato fibre-enriched breadOriginal Paper

Omar Saleh, Hossam S. El-Beltagi, Abdelrahman R. Ahmed, Hefnawy Taha, Haiam O. Elkatary, Abdalla El‐Hadary, Hoda A.S. Farag, Mahmoud Sitohy, Ahmed Mahmoud Ismail, Ali Osman

Czech J. Food Sci., 2025, 43(6):462-469 | DOI: 10.17221/29/2025-CJFS

This study investigates the effect of varying concentrations of potato fibre (PF) (1, 2, and 3%) on wheat flour (WF) dough rheological properties and the resultant pan bread quality. Chemical analysis, physical characteristics (weight, loaf volume, and specific volume), and colour attributes were estimated to assess bread quality. The impact of PF on bread sensory evaluation was estimated. The addition of PF significantly affected the properties of the dough. Moreover, the data indicated a substantial increase in water absorption (P ≤ 0.05) from 57.2% for the control to 65.5, 73.3, and 77.6% with the addition of 1, 2, and 3% PF to WF, respectively. Protein, crude fibre, and ash levels significantly increased (P ≤ 0.05) with higher PF ratios. Moisture and carbohydrate contents were markedly reduced (P ≤ 0.05). The specific volume considerably decreased from 3.15 cm3·g–1 in the control to 2.9, 2.74, and 2.53 cm3·g–1 with the addition of 1, 2, and 3% PF to WF, respectively. All replacement samples exhibited no significant alterations (P ≤ 0.05) in L* values and substantially (P ≤ 0.05) increased values of yellowness (b*) in all fortified samples relative to the control. Sensory assessments revealed that PF can be incorporated into WF at concentrations of 1% and 2% without compromising the overall acceptability of bread compared to the control.

Closed-circle bioeconomy: Applied aspects of agricultural implementationReview

Yelizaveta Chernysh, Viktoriia Chubur, Miroslav Hájek, Hynek Roubík

Agric. Econ. - Czech, 2025, 71(12):680-690 | DOI: 10.17221/36/2025-AGRICECON

In the face of global challenges, innovative methods of production and consumption are vital, and the bioeconomy signifies a transformational shift based on biological processes, minimal energy use and full integration of resources into ecosystems. This study focused on a sectoral analysis of bioeconomy opportunities with a focus on bioenergy and waste management in an agriculture sustainable approach. Literature-based analysis was used to approach the research objectives of the roadmap design methods. A comprehensive sectoral analysis of the bioeconomy was conducted with a special emphasis on bioenergy and food security. A conditional roadmap of bioeconomy implementation clusters was presented, which is in line with global trends.  Agriculture, being an integral part of the bioeconomy, faces challenges due to ecosystem degradation, water scarcity, and poverty. The global trend of biotechnology adoption in agriculture offers benefits for sustainable bio-economic development, including soil conservation, cost reduction, job creation and improved food quality. Thus, the bioeconomy has significant potential to address global challenges and achieve sustainable development, which requires innovation, regional adaptation and a commitment to harmonise economic growth with environmental conservation. Further research into the involvement of the rural areas in the development of bioeconomy is required.

Sorption isotherm modelling of dried tomatoesOriginal Paper

Ahmad Khalid Nayab, ¥ubomír Valík, Pavel Aèai

Czech J. Food Sci., 2024, 42(1):21-30 | DOI: 10.17221/109/2023-CJFS


The sorption isotherm (SI) of dried tomatoes was studied at three different temperatures, 15, 25, and 35 °C, using a static gravimetric method. The modified forms of the Guggenheim-Anderson-de Boer (mGAB), Halsey (mHAL), Henderson (mHEN), and Oswin (mOSW) models that incorporate the temperature term in their equation were selected and used to describe the experimental data of dried tomatoes. The mGAB model best described the SI of dried tomato samples at individual temperatures, having the highest coefficient of determination (R2) and the lowest sum of squares of errors (SSE), the root mean square error (RMSE), and the corrected Akaike information criterion values (AICc). However, based on the statistical indices, three other tested models outperformed the mGAB model in describing the multi-temperature estimation to differentiate the temperature effect. The mOSW and mHAL models were superior in this case.

Comparative study on the large-scale intensive culture of pikeperch (Sander lucioperca) larvae: Evaluation of two different live exogenous food optionsOriginal Paper

Václav Kuèera, Tomá¹ Pìnka, Oleksandr Malinovskyi, Carlos Yanes-Roca, Zhaxygali B. Kuanchaleyev, Tomá¹ Policar

Czech J. Anim. Sci., 2024, 69(6):243-254 | DOI: 10.17221/42/2024-CJAS

This study is focused on the comparison of production efficiency (growth, survival, and development) and economic evaluation of large-scale larvae culture in pikeperch (Sander lucioperca) using Artemia (Group A) compared to using rotifers and the subsequent combination of rotifers and Artemia (Group R) as a first exogenous feed following weaning and intensive culture of juveniles. Both experimental groups were stocked with the same initial density (100 pcs per litre) of larvae at 4 days post hatching (DPH). Each group was cultured in triplicate until 65 DPH. Assessed parameters included FBW (final body weight), FTL (final total length), SGR (specific growth rate), SR (survival rate), CR (cannibalism rate), SBI (swim bladder inflation rate), and TDR (total deformity rate). Production costs were confronted with total number of all produced and high-quality juveniles to determine the cost per one juvenile. Juveniles in Group A displayed more rapid growth (significantly higher FBW, FTL and SGR). On the other hand, parameters concerning quality of the fish (SBI, SR, Share of high quality juveniles and TDR) were significantly higher in Group R. Statistical difference in CR also benefits Group R. Production of bigger share of high-quality juveniles in Group R decreased overall production cost per one high-quality juvenile by 59.7%, from 0.72 EUR in Group A to 0.29 EUR in Group R. Use of established feeding protocols confirmed significant production and economic improvements in pikeperch larvae culture and production of high-quality juveniles. Even though the price of rotifer cultivation significantly exceeded the price of Artemia purchase and cultivation, the production cost of high-quality pikeperch juveniles significantly decreased.

Assessment of agricultural land salinization via soil analysis and remote sensing data: Case study in Pavlodar region, KazakhstanOriginal Paper

Dauren Rakhmanov, Boøivoj ©arapatka, Kamilla Alibekova, Jan Èernohorský, Petr Hekera, Zhassulan Smanov

Soil & Water Res., 2024, 19(2):111-121 | DOI: 10.17221/5/2024-SWR

Soil salinization is one of the most widespread soil degradation processes, especially in arid and semi-arid regions. In such climatic conditions, soluble salts accumulate in the soil, leading to deterioration in soil properties and ultimately reduced crop yield. The purpose of this study was to analyse the relationship between the level of soil salinity and the main spectral indicators obtained from Landsat satellite data. The studied area was the Maisky district, which is located in the southeastern part of the Pavlodar region of Kazakhstan. The variants of the research were agricultural lands using sprinkler irrigation and flood irrigation, as well as sites without irrigation. To analyse the relationships, we used the normalized difference vegetation index (NDVI), salinity indices (SI) and soil indices such as SI 1, SI 2, SI 3, SI 4, normalized difference salinity index (NDSI), soil adjusted vegetation index (SAVI), and brightness index (BI). The normalized difference salinity index (R-NIR)/(R + NIR), using a quadratic statistical relationship, showed the best correlation with the laboratory data. The vegetation index NDVI showed the weakest correlation due to dryness or poor crop growth. As a result of the lack of clear control over irrigation and agrotechnical measures, the indicators of cation exchange capacity in irrigated plots using the flooding method were higher than in other irrigation methods. During irrigation, it is necessary to ensure clear rules, according to which the supplied water and fertilizers will have a positive effect on the soil and the entire agroecosystem. The methods used in this research can be useful in mapping and studying saline soils using satellite data in natural and climatic conditions of arid and semi-arid regions.

Quality characterisation of cake glazes containing tropical fruit seed powdersOriginal Paper

Nurulhuda Md. Salleh, Norazlin Abdullah, Nurulain Syuhada Mohamad Yazid, Norhayati Muhammad

Czech J. Food Sci., 2024, 42(4):235-242 | DOI: 10.17221/54/2024-CJFS


This study aims to characterise the physicochemical properties and stability of confectionery glazes made from various tropical fruit seed powders during storage. Physicochemical properties were evaluated at regular intervals throughout frozen storage to monitor quality changes. The mango seed glaze is slightly acidic, and significantly darker, with tones of green and blue. The glaze has a total soluble solid content of 73.37 °Brix, a moisture content of 19%, hardness of 46.69 g, and sagging capacity of 18.33 mm, which ensures both structural integrity and sensory appeal. Its highest viscosity among formulations suggested possible thickening and improved stability. Over 28 days, significant changes occur, with the pH stabilising to a near-neutral 6.8. The hue changes over time, migrating towards reddish tone as indicated by increasing positive a* values, while maintaining a consistent preference for blue-toned hues with continuously negative b* values. Moisture levels remained stable at 18.71% during storage, with minimal total soluble solid content changes, indicating sustained quality. The findings suggest that mango seed glazing is a viable option for quality preservation during storage. This study provides essential empirical insights into the potential changes in the quality characteristics of various fruit seed glazes when subjected to freezing.

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