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Predators of aphids on apples were studied during the growing season in 2021 and 2022 in the region of East Sarajevo, Bosnia and Herzegovina. Eleven predator species were found on eight apple cultivars in the colonies of three aphid species, Aphis spp., Dysaphis plantaginea Passerini and Dysaphis devecta Walker. Seven species were identified from the family Syrphidae: Episyrphus balteatus (De Geer), Meliscaeva (Episyrphus) auricollis (Meigen, 1822), Eupeodes flaviceps (Rond.), Eupeodes corolae (Fabr.), Scaeva pyrastri (L.), Syrphus ribesii (L.) and Syrphus vitripennis Mg. The larvae of syrphids were more numerous in the Kula location, 99 (52 in 2021 and 47 in 2022), while their number in the Klek location was 59 (35 in 2021 and 24 in 2022). The most abundant species was E. balteatus (40.50%), followed by E. flaviceps (12.65%), S. ribesii (10.75%), M. auricollis (10.12%), S. pyrastri (10.12%) and S. vitripennis (6.96%). The family Coccinellidae is represented by three species: Adalia bipunctata L., Coccinella septempunctata L., and Harmonia axyridis Pallas. From the family Cecidomyiidae, one species Aphidoletes aphidimyza (Rondani), was detected. The largest number of predatory species (nine) was found on the Idared cultivar (in D. plantaginea colonies), where the colonies of this aphid species were the most abundant. The least numerous predatory species were identified on the Gloster cultivar in D. devecta and Aphis spp. colonies. E. balteatus was the most common and numerous of all the predatory species.

Evaluation of genetic resources of eggplant (Solanum melongena L.) from different geographical areas using molecular markers (RAPD) is of great importance in the breeding process. A total of 90 polymorphic amplified products were obtained from 10 decametric RAPD primers, used to analyse the genetic diversity of 20 genotypes of eggplant (16 local and 4 genotypes of foreign origin). The highest polymorphism was determined using the OPAF-16 primer (70.83%). The number of detected bands ranged from 13 (OPF-04) to 24 (OPAF-16), while the average number of bands per primer was 17.2. The lengths of the amplified fragments ranged from 400 to 9 000 bp. The value of the Jaccard’s genetic distance coefficient ranged from 0.095 to 0.35, and the dendrogram constructed using the UPGMA method showed that 16 local and 4 foreign genotypes were grouped into nine groups (clusters). Populations K1, K8/1, K19, K22, K25 and K38 represent genotypes that separated from the others and formed single clusters. The lowest value of the calculated genetic distance was 0.095 between domestic genotypes K13 and K12, which also showed morphological similarity in terms of shape and colour. On the other hand, the highest value of genetic distance was calculated between foreign genotypes K19 and K25 (0.35), K19 and K34 (0.34) and K19 and K38 (0.34). Genetically distinct genotypes identified using RAPD markers could be potential starting genetic material for crossing with other genotypes to obtain new and improved eggplant varieties.

Pumpkin seeds and their oil cake are often treated as by-products, but their composition and high nutritive and biological value makes them a promising material for nutritional enrichment of bakery products. The purpose of this study was to characterise pumpkin seed flour (PSF) and pumpkin seed oil cake flour (PSOCF), and evaluate the effects of their addition on the chemical composition including the fatty acid composition, textural parameters, sensorial characteristics and antioxidant activity of the wheat bread. Six experimental bread samples with 5, 10 and 15% of PSF or PSOCF added to wheat basic formulation, and a control wheat sample, were produced. Results indicated that addition of PSF and PSOCF led to higher ash, cellulose, protein, fat, total carotene and unsaturated fats content, which means that the experimental bread samples had a higher nutritional value. PSF addition had more positive effect on the textural characteristics and antioxidant activity of bread, than PSOCF addition. All bread samples (evaluated by 5-point scale multiplied with the coefficient of importance) had very high sensory quality (89.88–96.59% of maximal possible quality). The results confirmed that PSF and PSOCF are suitable for use in food industry for nutritional enrichment of wheat bread and related products.

Monitoring the genetic diversity in livestock is a critical component for sustainable management of small and endangered breeds. In this paper we follow up the two most often used metrics of genetic diversity, the average inbreeding coefficients (F) and the effective population (N_e) size in 16 sheep breeds from four countries. The analysis was based on single nucleotide polymorphism (SNP) markers, with about 35 to 49 thousand SNPs and 19 to 98 individuals after quality control. The runs of homozygosity (ROH) method with the cgaTOH software was used to estimate F_ROH, for three different time points in the past (3, 6 and 12 generations before present). Three methods were implemented to estimate N_e, using the NeEstimator v2, GONE and CurrentNe software for different time spans in the past. The average F_ROH ranged between 0.001 and 0.035 for 3 generations, 0.003 and 0.059 for 6 generations, 0.005 and 0.074 for 12 generations in the past. The wide range of inbreeding coefficients might be a consequence of different population sizes and breed management strategies in the respective countries. The results for N_e ranged from 7 to 352 for NeEstimator, 25 to 303 for GONE, and 15 to 542 for CurrentNe. Overall, the software showed comparable results for 10 out of 16 breeds, and significantly different results for at least one software for at least one of the methods for 6 out of 16 breeds. These differences show a degree of uncertainty in N_e estimations for certain breeds. Overall, we suggest the continued monitoring of sheep breeds, ideally by routine genotyping in all populations. This monitoring is especially important for small breeds with a greater decline in genetic diversity.

One of the latest environmentally friendly methods in soybean production technology is the pulsed electromagnetic field of low frequencies (PEMP). The paper presents the results of the influence of electromagnetic stimulation of soybean seeds on grain yield, protein and oil yield, depending on different agroclimatic conditions, exposure time and frequency. In the 2012–2017 research period, the soybean cv. Valjevka was used in the Institute of Field and Vegetable Agriculture experimental field, Novi Sad, Serbia. Immediately before sowing, the seeds were subjected to PEMP treatment, with a pulse generator and a tape applicator, in the following variants: electromagnetic field frequencies of 16, 24 and 30 Hz, and exposure time of 0, 30 and 60 min. The most successful variant of seed stimulation for all three examined parameters was at 16 Hz and 30 min, where the research results show that this measure can increase the examined parameters by more than 10%. The average yield of grain for all years of research with seed stimulation was 4.85% (3 338 kg/ha) compared to the control (3 203 kg/ha). The average grain protein yield in the treatments with PEMP was 1 315 kg/ha, which was 4.26% higher compared to the variant without PEMP (1 260 kg/ha), and the treatment was 4% higher in the average oil yield, 703 kg/ha compared to the control 676 kg/ha. Also, the analysis of the mutual dependence of the indicators is in a positive correlation, which is essential for plant breeding and the development of new technologies, which have economic justification, are safe for use and have a positive impact on adverse effects such as drought.

Resveratrol and polydatin are stilbenes with notable antioxidant and health benefits, making them important compounds in health promotion, grapevine production, and oenology. This study aimed to monitor the trans isomers of these compounds in twelve grape varieties (red and white), grown under organic production (OP) and conventional production (CP) systems. A novel high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) method was developed and validated, demonstrating sensitivity and reliability for the quantification of the mentioned stilbenes. The method was applied to real samples, enabling the evaluation of the influence of production systems on the presence of the analysed compounds. The research included some of the most widely cultivated domestic and international varieties in the examined region. The obtained results revealed a significant impact of the production system and grape colour on resveratrol and polydatin content. OP consistently yielded a higher content of both compounds, compared to CP. Furthermore, red varieties showed higher overall stilbene levels than white ones. ‘Shiraz’ variety was an exception, with high levels of both compounds under CP. All the obtained findings highlight the role of the production system in enhancing bioactive compound content in grapes. The study provides valuable insights for optimising viticulture, supporting plant resilience, and improving the nutritional qualities of grape-derived products.

The study tested the effect of daily feed ration (DFR: 0.5%, 0.75%, 1.0%, 1.25%, and 1.5% of fish biomass) on juvenile pikeperch (Sander lucioperca) with an initial body weight of 21.5 ± 5.1 g and total length of 144.5 ± 8.5 mm. The pikeperch were fed floating feed at 8-hour intervals for a duration of 84 days. By the end of the experiment, the group fed DFR of 1.5% exhibited the highest body weight (51.5 ± 16.1 g) and total length (188.2 ± 17.8 mm). The weight heterogeneity, measured as the coefficient of variation (CV), ranged between 30.29 and 33.24%. The specific heterogeneity rate (SHR) ranged from 304.44 to 334.94‰/day. The group with DFR of 1.0% exhibited the highest degree of heterogeneity. Minor fin erosion was observed in the caudal fin by the end of the experiment. No significant differences were revealed in selected biochemical parameters indicating the liver, spleen, and intestinal function. All the fish tested were adequately fed, being provided sufficient nutrients for the proper growth of pikeperch. The DFR of 1.5% was evaluated as the most favourable. This amount of feed supported a higher number of values for Fulton’s coefficient (FC), specific growth rate (SGR), thermal growth rate (TGR), fish weight heterogeneity, growth of total fish biomass (BG), and optimum level of biochemical parameters in blood plasma.

Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are causative agents of the porcine respiratory disease complex. However, information on the prevalence of these pathogens in wild boars is scarce. This study aimed to investigate the presence of antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in wild boars in Serbia. In this study 253 serum samples from wild boars were tested for antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumonia using the ELISA assay. The overall seroprevalence rates of Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae were 4.2% and 56.9%, respectively. Antibodies to both pathogens were detected in 20 sera samples (7.9%). The prevalence of wild boars that were seropositive for Mycoplasma hyopneumoniae differed with age and ranged from 10.7% to 33.3%, and for Actinobacillus pleuropneumoniae, it ranged from 51.8% to 83.3%. Wild boars are hard to control and are considered a high-risk infection source for outdoor and backyard pigs and eventually for commercial indoor farms as well. Thus, the result of this first serosurvey in Serbia should raise awareness of the importance of wild boars as potential reservoirs of bacterial pathogens such as Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. Our data revealed the circulation of both pathogens in wild boars in Serbia, drawing attention to the potential health risk they present for domestic swine health.

Coppice is the oldest form of systematic and sustainable use of forests, and is currently applied on about 29 million ha (about 14% of total forest land area) in Europe. It had its maximum spread in the 16^th century, when an estimated 36% of all forested land in Europe was managed for coppice. Coppice forests were the most important source of fuelwood until the mid-19^th century, when firewood and charcoal were substituted by alternative fuels, and the demand for construction wood increased. Consequently, coppices (both low and coppice-with-standards) started to be converted to high forests, and the process was driven by national policies and/or subsidies, which is still the situation in some European countries. During the 20^th century, coppicing was abandoned in many places across Europe due to the abandonment of the countryside and population migration into cities, as well as changes in socio-economic conditions, technical advances and political restrictions. However, coppicing is still important in many European countries as the main source of firewood for the rural population, who has limited access to other sources of energy. In this context, this paper presents the most important characteristics of this complex abandonment/management/conversion picture, emphasising the pros and cons for the future of coppices across Europe.

This study systematically investigated the synergistic improvement of expansive soil using organic fertiliser (OF), slow-release fertiliser (SRF), and rice straw (RS) through Box-Behnken design (BBD) and response surface methodology (RSM). Key findings include: the quadratic models demonstrated high statistical significance (root density: R² = 0.765, F = 25.84; shear strength: R² = 0.885, F = 18.65; swelling rate: R² = 0.20, F = 15.23; all P < 0.001) with low prediction errors (root content: ± 0.08 mg/cm³; shear strength: ± 0.58 kPa; swelling rate: ± 0.38%); The combination of 12.30% OF + 0.7 kg/m³ SRF + 0.4% RS achieved 58% improvement in shear strength, 32% improvement in root content, 42.7% reduction in swelling rate; OF exhibited negative linear effects on root density (β = – 0.18, P = 0.002) with >10% dosage reducing root growth by 9.0%; SRF showed positive linear impacts on shear strength (β = +0.25, P = 0.001) and root density (β = + 0.12, P = 0.023); RS enhanced shear strength below 0.5% (β = + 0.08, P = 0.042) but impaired root density due to pore clogging (β = – 0.15, P = 0.008). The optimised formulation, validated by triplicate centre-point tests (coefficient of variation ≤ 2.1%), is recommended for slope stabilisation while limiting OF to ≤ 10% to prevent performance degradation. This data-driven approach provides actionable insights for balancing agricultural waste utilisation and geotechnical performance in expansive soil improvement.

Tomato is a major global crop, extensively cultivated in China. However, the molecular mechanisms underlying its responses to high-temperature stress remain poorly understood. This study investigates these mechanisms by examining a heat-resistant tomato variety, Hm 2-2 (R), and a heat-sensitive variety, BY 1-2 (S), under high temperature (40 °C). Total RNA was extracted from samples taken at 0 and 24 h post-treatment, followed by RNA-sequencing (RNA-seq). Differentially expressed genes (DEGs) were screened based on the criteria of |log₂ fold change| ≥ 2 and false discovery rate ≤ 0.05. Gene ontology (GO) function annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway enrichment analysis were performed to explore the biological significance of these DEGs. The results revealed 6 038 upregulated and 2 866 downregulated DEGs in the R-0 (Hm 2-2 plants treated at 40 °C for 0 h) vs. R-24 (Hm 2-2 plants treated at 40 °C for 24 h) group and 5 297 upregulated and 3 920 downregulated DEGs in the S-0 (BY 1-2 plants treated at 40 °C for 0 h) vs. S-24 (BY 1-2 plants treated at 40 °C for 24 h) group, respectively. GO enrichment analysis indicated that the majority of DEGs were associated with biological processes, followed by cellular components and molecular functions. KEGG pathway analysis identified 130, 131, 89, and 115 regulatory (or altered) pathways in the R-0 vs. R-24, S-0 vs. S-24, S-0 vs. R-0, and S-24 vs. R-24 group comparisons, respectively. Notably, pathways related to protein processing in the endoplasmic reticulum and plant hormone signal transduction were significantly enriched, suggesting their critical roles in the tomato’s response to heat stress. Moreover, 156 transcription factors (TFs) implicated in heat stress response were identified, spanning various TF families such as MYB, AP2-EREBP, b-ZIP, bHLH, NAC, and WRKY. Quantitative RT-PCR analysis of 14 randomly selected DEGs validated the RNA-seq results confirming the reliability of the data. In summary, this study provides valuable insights into the molecular mechanisms of tomato’s responses to high-temperature stress, laying a crucial foundation for future research in this area.

Although the effects of fertiliser addition and liming on semi-natural grassland productivity and biomass quality are well documented, less is known about how fertilisers change plant functional groups and mean ecological values. We researched the effects of liming (no lime and lime with 1 t/ha) and mineral fertilisers (control – no fertilisers, PK-P60K60, N20PK-N20P60K60, N80PK-N80P60K60, and N140PK-N140P60K60) for nine years on the Danthonia alpina Vest. grassland community. Based on Brown-Blanquet cover abundance, we calculated Shannon-Wiener evenness and abundance of plant functional groups (based on height, canopy structure, storage organs presence and flowering duration). We also researched Landolt’s ecological indicator values for nutrients, moisture, reaction, light, and temperature. Results revealed that fertilisers stimulated tall species with longer flowering duration. Shannon-Wiener evenness in control was 0.45, and N20PK increased to 0.71 but significantly decreased in treatment N140PK (0.25). Mean Landolt ecological value for nutrients and moisture increased while temperature dropped. The coverage of legumes and Landolt indicator value for nutrients increased because of the lime application, while the lime had no effect on Shannon-Wiener evenness and abundance of functional groups. Greater Shannon-Wiener evenness in treatments of PK and N20PK is a prerequisite for resistance to the effects of extreme climate events.

In this article, we explore multiscale extreme risk interdependence between four soft agricultural markets – coffee, cocoa, cotton and orange juice. Wavelet correlation and cross-correlation are used to investigate this interlink, and dynamic conditional Value at Risk is used to measure extreme risk. Wavelet correlation results suggest a very weak connection between the markets in the short-term and midterm horizons, which means that investors who operate in the short term or midterm do not have to apply hedging measures against extreme risk. However, the situation is different in the long term, where relatively high correlations are found on the highest wavelet scale in all pairs, except coffee–cocoa. Complementary cross-correlation analysis indicates a lead–lag relationship between the markets. The results are mostly in line with expectations, as bigger markets lead smaller markets. Only in the cases of cocoa–cotton and cocoa–orange juice does the opposite happen.

The aim of the research was to examine the influence of different light treatments on the growth, phytochemicals and antioxidant potential of broccoli microgreens. Plants were grown in a growth chamber under LED (light-emitting diode) cold white, red and blue light and under fluorescent cold white light (control). The results showed that white and blue light treatments were the best for microgreen growth. Higher concentration of pigments was recorded in plants grown under LED light compared to those grown under FL (fluorescent lamp) light. The content of phenols and flavonoids had a positive and significant correlation with DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidative capacity (r = 0.66 and r = 0.90, respectively). The first two principal components account for 97.92 % of the total variation of all observed traits in this trial. Based on the PCA (principal component analysis) results, it can be concluded that the traits total phenols content, carotenoid content, chlorophyll a and b content make up the largest share of variability in the obtained results and that the red light conditions were the most unfavourable for the content of phytochemical compounds and antioxidant potential.

This work aimed to evaluate the influence of soil characteristics and the applied amount of nicosulfuron on the degradation rate in soil. Soil samples were collected at three localities in Bosnia and Herzegovina – Manjača, Kosjerovo and Tunjice. The experiment was carried out under controlled laboratory conditions. Plant protection product based on nicosulfuron (40 g a.s./L, OD) was applied in concentrations of 0.075, 0.15, and 0.30 mg a.s./kg of soil. Nicosulfuron residues were analysed by the modified QuEChERS method, followed by LC-MS/MS. Soils are classified as silty loams, with variations in mechanical composition and chemical properties. In slightly alkaline soil, the half-life (DT50) of nicosulfuron has increased (43.31 days) compared with DT50 (9.43–16.13 days) in acidic soils. The results indicate that soil characteristics and applied concentration significantly influence nicosulfuron persistence. Hence, it can be considered that nicosulfuron, applied to silty loam soils of Bosnia and Herzegovina, poses a low risk to subsequent crops and the environment.

The main goal of this study was to evaluate the concentration and release dynamics of molecular hydrogen (H₂, dihydrogen) in dietary evaluate supplements and identify products that provide a biologically significant amount of dihydrogen suitable for human consumption. We examined ten commercial supplements marketed for their dihydrogen content, including slow-release capsules and tablets (4 products), effervescent powders and tablets (5 products), and canned ready-to-drink beverage (1 product). These products were acquired either through online purchases, from retail stores, or obtained free of charge directly from the manufacturers upon request. Dihydrogen concentration was measured using a highly sensitive Clark-type hydrogen microsensor with a detection limit 0.05 µmol·L^–1. Out of the ten products examined, only three (30.0%) exhibited dihydrogen levels surpassing the levels marketed as biologically relevant (500 µmol·L^–1), and one of these products (a canned ready-to-drink beverage) approached this level with a concentration of 439.2 µmol·L^–1. Interestingly, all slow-release capsules yielded negligible amounts of hydrogen (< 2 µmol·L^–1), while a slow-release tablet delivered 43.6 µmol·L^–1 of dihydrogen per single dose. The substantial variance in dihydrogen content among the assessed supplements holds significant implications for the general public, as high-potency products have the potential to provide up to 7 000 times more dihydrogen per single dosage compared to their low-potency counterparts.

We investigated, under field conditions and during four years (2018–2021) the effects of five irrigation levels (T1: 100% of crop water requirement; T2: 80% of T1; T3: 60% of T1; T4: 40% of T1, and T5: 0% of T1 – rainfed) in interaction with three planting densities (PD1: 54 900, PD2: 64 900, and PD3 75 200 plants/ha) on the yield, yield components and water use efficiency (WUE) of maize in Srem, Serbia. The results indicate a large year-to-year variability, mainly due to the total amount and distribution of rainfall. Water regime and PD interacted significantly. Irrigation increased grain yield 28, 34, 30 and 18% for treatments T1, T2, T3 and T4, respectively, compared to the T5; and significantly influenced the yield components. Planting density had significantly lower effects on grain yield compared to irrigation (+1.4–1.8%). WUE is maximised (3.436 kg/m3) at T4 under 75 200 plants/ha. Grain yield and WUE increased significantly with increasing PD, while the number of grains per ear and the weight of 1 000 grains decreased with increasing PD. In conclusion, limited irrigation at T2 under PD2 may be a viable method to maximise production efficiency and maize yield under the environmental conditions of this study and at sites with similar soil and climatic conditions.

This study was conducted to evaluate the efficacy of yeast cell wall parts (YCW) in reducing the severity of coccidiosis in broiler chickens. One-day-old Ross 308 chicks (n = 320) were randomly allocated to 32 floor pens in two rooms with a 2 × 4 factorial arrangement of treatments. Factors were (1) challenge: negative or positive by room, (2) feed additive: control (none), anticoccidial (salinomycin at 60 mg/kg), YCW 0.1% or 0.2%. On day 8, none-challenged room was inoculated with saline, and challenged room was inoculated with 5 000 Eimeria tenella oocysts. Performance results showed that in challenged birds, feed conversion ratio (FCR) and weight gain (WG) were poorer than in unchallenged birds on day 24 and 35. Birds given anticoccidial and YCW had improved FCR and WG (P = 0.01) over the control group. Challenge and additive interactions were observed on day 24 and day 35 for FCR and WG (P = 0.01) and for feed intake on day 24 (P = 0.01). Challenged birds fed YCW on days 24 and 35 had higher WG and better FCR compared with both challenged and non-challenged controls and anticoccidial treatment. Coccidial challenge increased crypt depth (CD), villous tip and base width, and villous surface area and decreased villous height to crypt depth ratio (VH : CD) and villous height (VH). Birds fed YCW exhibited significantly decreased CD, villous tip width, villous base width, villous surface area and increased VH, VH : CD in comparison to the control group. Challenged birds fed YCW had significantly lower bursa of Fabricius follicle length compared to birds fed the control diet. Challenged birds had significantly increased serum alanine transaminase (ALT) and aspartate aminotransferase (AST) concentrations than non-challenged birds. In conclusion, these results demonstrated that the yeast cell wall has the ability to control coccidiosis.

This research evaluated the suitability of commercially available larval feeds, Otohime B2 (OB2), Aller Infa (AI), and Aqua Start (AS), and one Experimental Feed (EF), for the weaning of largemouth bass (Micropterus salmoides), LMB. Feeds were presented with various ω-3 fatty acid levels/bioavailability (high in OB2 and AI), fat percentage (high in OB2 and AS), free amino acid and short peptide (FAA + SP) levels (high in OB2), and various soluble protein (SPR) levels (high in AS and EF). Fish were co-fed Artemia plus OB2 from the 19^th to 22^nd day post-hatching (DPH), then Artemia in addition to one of the four above diets for seven days, with complete Artemia removal on the 30^th DPH. Fish were sampled on the 32^nd DPH. Morphometry, digestive enzyme activities, hormonal status, skeleton, muscle development, and potentially pathogenic Flavobacterium spp. levels were estimated. Survival was high (96% or more) in all the weaning regimes. Weaning to OB2 was linked to a fast fish growth rate (14.29%/day), while both OB2 and AI supported the skeleton development. Weight gain correlated with total fat, ash levels, free amino acids, and short peptide levels in the diet. Larvae weaned to soluble protein-rich AS and EF showed the lowest fish weight gain and skeleton development, and lower growth of potentially pathogenic Flavobacterium spp. This research suggests that the weaning diets for largemouth bass should have a balanced protein content and quality while allowing for the inclusion of fewer marine ingredients.

The present study aimed to assess the effects of thermal manipulation on the vitellogenesis and final oocyte maturation (FOM) in pikeperch. Two groups of fish were stocked in two separate tanks of the climate chamber. One group was stocked at 10-month age (THERMAL), while the other was continually kept under stable photothermal conditions until 19-month-age (CONTROL) and then transferred to a climate chamber. The progress of vitellogenesis was assessed via evaluation of the oocyte diameter at the mid- and late-autumn phases, and the gonadosomatic index (GSI) at the late-autumn phase. Finally, females from the CONTROL group were hormonally stimulated before (WARMING) and after (STABLE) increase of the temperature from wintering to spawning, and the FOM progress, ovulation and plasma levels of 17 α, 20β dihydroxy progesterone (DHP) were assessed. Significantly larger oocytes at the mid-autumn phase (878.8 ± 40.1 μm vs 836.5 ± 46.5 μm) as well as the GSI at the end of the autumn phase (10.5 ± 1.7% vs 7.6 ± 1.1%) were noticed in THERMAL fish. Significantly faster FOM was seen in fish under the WARMING post-stimulation regime, and these fish had higher DHP levels at the moment of hormonal stimulation (5.4 ± 1.4 ng/ml vs 3.8 ± 1.2 ng/ml). According to the obtained results, it appears that photothermal induction of fish at a younger stage might have a positive impact on the first spawning, while the WARMING thermal regime seems to be more efficient in stimulating the FOM in fish upon first wintering.

This study investigated the effects of slaughter weight and breed on carcass characteristics, meat quality, and technological properties in purebred Balkan and F1 crossbred (Balkan × Saanen) male goats. A total of 48 animals, divided into four slaughter weight groups (10, 15, 18, and 22 kg), were analysed for carcass composition, muscle traits, and fat distribution. ANOVA and multivariate analyses (PCA and HCA) revealed significant effects of weight and breed on key carcass and meat quality parameters. Heavier animals exhibited higher intramuscular fat and primal cut yields with superior water-holding capacity (WHC), while lighter goats had leaner carcasses with less muscle pigment. Crossbred F1 goats demonstrated a more balanced muscle-to-fat ratio, whereas pure Balkan goats tended to accumulate fat more rapidly at higher weights. These findings indicate that optimal slaughter weight selection should align with market demands, such as producing leaner meat in lighter Balkan goats, or achieving increased marbling and better economic returns in heavier crossbred animals. Future research should explore genetic effects on muscle and fat deposition and assess consumer preferences to refine breeding and production strategies.

Drosophila suzukii poses a significant threat to berry fruits with its uncontrolled spread. Essential oils (EOs) have emerged as potential bioinsecticides due to their natural origin, mode of action, and biodegradability. Although EOs show potential for use in agriculture due to ecotoxicologically favourable characteristics, additional research is required to enhance their effectiveness, stability, and application for practical implementation in pest management. The primary objective of this research was the development of a bioinsecticide formulation based on a combination of three EOs – Pelargonium graveolens, Anethum graveolens, and Pinus sylvestris followed by the assessment of formulated bioinsecticide physicochemical properties. Using a two-choice bioassay, this study aimed to evaluate the effects of formulated bioinsecticides on D. suzukii, regarding their insecticidal properties through oviposition deterrence. The developed formulation exhibited favourable physicochemical properties and demonstrated a decrease in the number of larvae in fruits. Bioinsecticides present an environmentally friendly approach to pest control. However, further research and development are imperative to fully exploit their potential for effective crop protection in the field, followed by comprehensive research to evaluate the potential side effects on natural enemies, ensuring that their implementation doesn't harm beneficial organisms and maintain ecological equilibrium.

Lactoferrin (LF) is an important bioactive component of colostrum, is critical for the development of immunity in the newborns, and it is an important component of the mammary gland defence system. LF is also an important biomolecule in terms of promoting and restoring the human health. The aim of our study was to monitor the dynamics of changes in lactoferrin concentration in goat milk at varying stages of lactation and its correlation with selected components and physicochemical parameters. Colostrum (n = 24) and milk (n = 120) samples were obtained from 12 goats by hand milking. Lactoferrin was determined using reversed-phase high-performance liquid chromatography with an ion-pairing reagent equipped with a Photodiode Array Detector. The LF concentration in colostrum ranged from 206 mg/l to 1 228 mg/l, and showed a statistically significant decrease in concentration (P < 0.05) over the colostral period. Significant correlation coefficients (P < 0.001) were found between crude protein and LF (r = 0.896), lactose and LF (r = –0.754), as well as between non-fat solids and LF (r = 0.853). The LF content in milk ranged within a relatively wide range of 94 mg/l to 1 115 mg/l although the values were highly variable (vx = 57.0%). Significant correlations were found between fat content and LF in milk (r = 0.429, P < 0.001), crude protein and LF (r = 0.376), non-fat solids and LF (r = 0.361), somatic cell count (SCC) and LF (r = 0.330), as well as log SCC and LF (r = 0.348, P < 0.01).

Riparian vegetation plays a major role in maintaining biodiversity and reducing the negative impact of nutrient leaching into aquatic ecosystems. However, the knowledge on the interactions between riparian vegetation and other environmental factors is still incomplete for planning sustainable riparian forest management. The aim of this study was to explore interactions between riparian forest ecosystem components along a small stream. Interactions between vegetation structure, chemical composition of soil and groundwater, as well as chemical element flows via litterfall and precipitation were studied in seven 50 m long transects located in the riparian forest of different characteristics along a 1.4 km river section in the northern part of Latvia. Our results showed that throughfall input of total nitrogen (TN) and potassium (K) was higher in transects with predominantly deciduous tree stands, but the concentration of TN in forest floor was higher in coniferous tree stands. At some soil layers, a positive correlation between organic soil carbon (OC) and the concentration of TN in groundwater was detected. The concentration of TN and nitrate-nitrogen (N-NO₃^–) in groundwater correlated positively with the deciduous tree basal area. The obtained results suggested that element flows are strongly dependent on tree species' composition and a comparatively small riparian area is able to provide diverse ecological conditions.

In Eastern Europe, near-natural forest patches are decreasing and are gradually replaced by non-native plantations. Tree plantations are commonly thought to be simple ecosystems with low conservation value, although this conclusion is mainly based on simple taxonomic diversity indices, which ignore functional and phylogenetic diversity. In this study, our objective was to compare species composition, diagnostic species, taxonomic, functional, and phylogenetic diversity, as well as naturalness status between two near-natural forest types (Quercus-Tilia and Populus alba) and two common plantation types (non-native Pinus sylvestris and non-native Robinia pseudoacacia) in the Deliblato Sands, Serbia. Our results showed that the species composition significantly differed in the four habitats. Each habitat had some species that were significantly concentrated in them. Most of the diagnostic species in the Quercus-Tilia forests were forest specialist plant species, while those in Populus alba forests were species associated with warmer and drier habitats, whereas the plantations hosted diagnostic species with broader ecological tolerances. Native species richness, total species diversity, and functional and phylogenetic diversity were similar in the four studied habitats, which can be explained by the combined effects of light regime and naturalness. We assessed low naturalness (i.e. high degradation) in plantations, which can be expected to reduce diversity. However, higher light availability was probably able to compensate for this effect. Non-native plantations, especially Robinia pseudoacacia plantations, were the most degraded and hosted the highest non-native species richness, implying that they are ecologically undesirable. In light of our results, we suggest that near-natural forest stands should be protected and efforts to restore these forests should be given high priority. Furthermore, it is advisable to continue with a forestry strategy that involves replacing non-native plantations with native ones, such as Tilia tomentosa, in the Deliblato Sands.

The use of genomic data makes it possible to examine genetic variability and calculate the genetic parameters of the population in an efficient and precise way. The aim of this research was to analyse linkage disequilibrium (LD), contemporary effective population size (Ne_c), haplotype block structure, minor allele frequency (MAF), observed (H_O) and expected heterozygosity (H_E), calculate the genomic relationship matrix and perform a principal component analysis (PCA) in the Serbian Holstein-Friesian cattle population using SNP data from the GGP Bovine 100K chip. After quality control (QC), 83 208 SNPs and 1 575 cows were retained for further analysis. LD on autosomes had an average value of ≥ 0.2 up to a distance of 50–60 kb (r² = 0.211), while on BTX r² ≥ 0.2 was represented at distances of 80–90 kb (r² = 0.211). LD differed between chromosomes. The average H_O for autosomes and X chromosome SNPs was 0.412 and 0.422, respectively. 81.30% of SNPs that passed QC had MAF > 0.2. The total number of haplotype blocks in the studied population was 15 642. On average, blocks contained 2.932 SNPs. The average block length was 32.657 kb and ranged from a minimum of 0.019 kb (BTA21 and BTA26) to a maximum of 999.562 kb (BTX). The estimated value of Ne_c in the this cattle population was 142. The results of PCA showed a significant variability of genotypes in the population, but there was no clear stratification of the population. The obtained results will serve as a basis for future genomic analyses such as the detection of QTLs for important economic traits and the implementation of genomic selection.

Cryopreservation reduces the antioxidant activity of spermatozoa and makes them more sensitive to damage caused by reactive oxygen species (ROS). The addition of antioxidants to the freezing medium could prevent cryo-damage by mitigating the harmful effects of ROS and, thus, protecting the spermatozoa. This study aimed to evaluate the effect of ellagic acid (EA) on the rabbit sperm traits after freezing-thawing. Semen samples collected from New Zealand White rabbit males were cryopreserved in a BotuCrio freezing medium (Nidacon, Sweden) supplemented with different concentrations of ellagic acid (EA at 0, 0.5, 1.5 and 2.5 mM) using the manual slow freezing procedure. After thawing, sperm motility parameters were evaluated by CASA. The parameters of viability (DRAQ7), apoptosis (Yo-Pro-1), acrosome integrity (peanut agglutinin; PNA), intracellular ROS (CellROX) and mitochondrial activity (MitoTracker) were evaluated by flow cytometry. EA added to the freezing medium at all concentrations led to a significant reduction (P < 0.05) in intracellular ROS in frozen-thawed sperm cells. However, this effect was not reflected in motility parameters. Semen supplemented with 1.5 mM EA also yielded a lower proportion of apoptotic cells compared to the control group. In conclusion, EA supplementation of semen extender demonstrated its antioxidative properties protecting spermatozoa against oxidative damage during cryopreservation. Nevertheless, to draw a definitive conclusion regarding the effect of EA on spermatozoa functionality, additional research is necessary.

This article provides a review of recent studies on the extent to which the use of organic acid elicitors such as salicylic, jasmonic, humic and ascorbic acids has been successful in alleviating the exposure of Lamiaceae plants to unfavourable environmental conditions such as drought and salinity. Overall, the results concluded all organic acid elicitors enhanced the morphological and physiological characteristics of biochemical and secondary metabolite contents. These improvements have enabled plants of the Lamiaceae family to adapt to environmental stress conditions to some extent and survive, thus achieving sustainability in the production of plants of this family. It can be recommended to use salicylic acid in concentrations 0.5–2.5 mM, and it should not exceed it so as not to cause poisoning and disruption of the vital and physiological processes within the plant. In contrast, these plants have limited studies on the relationship between jasmonic acid/ascorbic acid and ascorbic acid. Since vitamins such as ascorbic acid are essential for plant metabolism and growth regulation, their effect on these plants remains unstudied at concentrations 2–10 mM under different abiotic stresses. Further research is needed to understand the impact of Nano-SA, JA, HA, ASA, and citric acid on Lamiaceae plants under various environmental stress conditions. Limited studies exist on the relationship between jasmonate/humic acid and Lamiaceae plants under abiotic stress. The Lamiaceae family needs more studies on adaptation to various environmental conditions and the toxicity of stimulants used to confront these conditions. This research contributes to improving agricultural practices in challenging environmental regions.

Common ragweed – Ambrosia artemisiifolia L. (Asteraceae) is an invasive plant species in Europe native to North America. Most of the records of known eriophyid mites on different ragweed species are from their native range. Our field experiments in Slovakia, 2016–2023, aimed to identify specific species feeding on common ragweed. We searched for symptomatic plants and collected growing tips, which were then preserved in 70% ethanol for further study. A recently described species of eriophyid mite, Aceria artemisiifoliae Vidović & Petanović (Acari: Eriophyoidea), was found in western and eastern Slovakia. This is the first record of the species in Slovakia and the second record in the world. It remains unclear whether this species is invasive like Ambrosia, and whether it could be used as a potential biological control agent.