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The objective of the present work was to evaluate the efficacy of use of the RITA^® temporary immersion system in the large-scale propagation of P. patula. The effects of four concentrations (0.00 µM, 4.50 µM, 9.00 µM, and 13.51 µM) of 6-benzylaminopurine (BAP) on 10 hypocotyl explants were studied using a completely randomised design with three replicates per treatment. Five hypocotyl explants were grown in 250 mL RITA^® containers of Woody Plant Culture Medium (WPM) supplemented with 20 g·L^–1 sucrose and 10 mg·L^–1 vitamins from Murashige and Skoog (MS) culture medium. The frequency of immersion of the explants into the culture medium was 2 min every 8 hours. The number of adventitious buds and calli formed, as well as shoot growth, were evaluated after 6 weeks of in vitro culture. The 4.50 µM concentration of BAP was the best treatment for shoot production (5 shoots per plant) and shoot length (1.32 cm). These results could help the widespread vegetative propagation of this important forest species.

Previous studies of the effects of elevated CO₂ (eCO₂) concentrations and heavy metal stress conditions on plant growth and physiology have mostly focused on crops rather than vegetables. In this study, we investigated the effects of Cd stress on the growth and antioxidant system of pak choi (Brassica rapa L.), water spinach (Ipomoea aquatica Forssk.), cherry radish (Raphanus raphanistrum subsp. sativus (L.) Domin) and pepper (Capsicum annuum L.) growing in pots under ambient CO₂ (aCO₂) or eCO₂ conditions. In general, Cd stress reduced plant biomass and SPAD values under aCO₂ and eCO₂ conditions; however, the reduction was smaller under eCO₂. Cd stress significantly reduced vegetable superoxide dismutase (SOD) and catalase (CAT) activities under both aCO₂ and eCO₂ conditions; however, the decrease in cherry radish and pepper peroxidase and SOD activities and in pak choi SOD and CAT activities was significantly less under eCO₂. The Cd content of the edible parts of pak choi, water spinach and pepper was significantly lower under eCO₂ than under aCO₂. Our data suggest that eCO₂ concentrations could be beneficial for the growth of some vegetables and reduce the Cd content.

Food security is of great importance to all countries. Correspondingly, agricultural price insurance is an important tool to maintain food security. This study adopts the traditional spider web model to establish a mathematical framework for exploring the internal mechanism of agricultural price insurance, which can ease the volatility of the agricultural market. Then, the influence of agricultural price insurance on the supply of agricultural products is examined. The findings show that the supply elasticity of most agricultural products is greater than the demand elasticity, with agricultural product markets presenting a natural divergence. Agricultural price insurance changes the supply curve of agricultural products by reducing their supply elasticity, subsequently positively affecting the reduction of price fluctuation and the stabilisation of outputs. Agricultural price insurance can even change agricultural product markets under certain conditions, allowing a shift from divergence to convergence. Moreover, by adjusting the insurance parameters of agricultural prices, the equilibrium yield and price can be changed, and the planting area and income of farmers can be maximised. The mathematical basis for agricultural insurance derived in this study can support food security strategies at the national level and further provide a theoretical basis to formulate policies and departmental measures.

The negative consequences faced by the entire planet, the intensification of natural disasters, global warming, lowering the level of groundwater, deforestation, deterioration of corals and the underwater world, soil degradation, and the change of species diversity, testify to the necessity of introducing reforms in the agricultural-food complex. Fulfilling such a goal and creating added value of innovation potential can only be created in the conditions of functioning structures strengthened by system support, which requires reviewing and introducing change in food systems. The recent financial crisis, later the pandemic, and the current going military conflict in Ukraine, caused problems in supplier-customer relations, and made humanity feel how the issue of food security and self-sufficiency can be easily and quickly disrupted. If farmers and food producers are to be able to respond to challenges and at the same time ensure the EU’s strategic goals, it is necessary to have support in research and innovation. Agricultural research and innovation in the EU is characterised by a long-term strategic approach that focuses on creating value from the land through sustainable primary production and strengthening rural innovation. It is an important tool in the fight against challenges such as climate change, environmental degradation, and biodiversity loss. In view of the above, it is necessary to transform food systems, apply regenerative agriculture, introduce alternative land-saving farming systems, observe the principles of sowing procedures and greater diversification of crops in the sowing procedure, reduce the consumption of agrochemicals, naturally integrate plant and animal production into agricultural business entities in relation to the circular economy, use farm waste for bioenergy production, protect agricultural land, implement measures to retain water in the country and support regions

This study analyses the perception of the relationship between forest certification and the production of ecosystem services by Programme for the Endorsement of Forest Certification (PEFC) and Forest Stewardship Council (FSC) sustainable forest management (SFM) certification holders. In addition, the psychological constructs that govern the use of certification for ecosystem services are investigated. Specifically, online questionnaire surveys were submitted to a sample of Italian and German forest owners and managers to study reasons for and against and global motives to adopt certification for ecosystem services through the application of Behavioural Reasoning Theory (BRT). Results show that respondents believe that certification can better support ecosystem services related to 'regulation and maintenance' and the conservation of biodiversity. On the other hand, the application of BRT has only been partially useful in explaining the psychological factors towards the adoption of ecosystem services certifications. In any case, attitudes and reasons for certainly had a significant influence on the intention to adopt certifications. Apart from the scientific implications, these results have practical applications for policymakers who can focus on the dissemination of the certification of ecosystem services by trying to support through eco-policies, the attitudes and reasons for expressed by forest owners.

Bone cysts are rare orthopaedic problems in dogs. There are no clear treatment guidelines. A young male Shih Tzu was referred to Small Animal Clinic with fifth-degree lameness (5/5) of the left thoracic limb, and with swelling and deformation of the distal humeral region. The radiological assessment revealed an enlargement of the distal brachium and an extensive hypodense osteolytic lesion in the distal metaphyseal region of the humerus. Diagnosis of the bone cyst was formulated and treated with a mini-invasive method using autologous non-concentrated bone marrow injections. The treatment was successful, and at the three weeks, the cyst significantly changed its structure. The follow-up at 12 weeks after the first injection, and at one year revealed complete recovery. To our knowledge, this is the first evidence of a bone cyst in a young dog successfully treated with a minimally-invasive method by using a non-concentrated autologous bone marrow injection.

The last years have seen a momentous transformation in Common Agricultural Policy (CAP). The focus of interventions has shifted from production toward rural development but recognising the role of farmers. Although the attitudes of farmers to CAP have long been previously studied, little attention has been focused on the views of citizens. Using data from the ‘Special Eurobarometer 520: Europeans, Agriculture and the CAP’, we sought to provide a clearer understanding of citizens’ opinions about the future of agriculture and rural areas in the European Union. In particular, we assessed rural-urban differences and examined disparities across countries, paying attention to the importance of rural development in the planned expenditure under CAP 2023–2027. The results showed that the perceived importance is higher for respondents living in rural areas and that there are disparities across countries. From a policy perspective, targeted efforts should be made in each country to address the specific challenges each country faces.

The city longhorn beetle (Aeolesthes sarta) (CLB) (Coleoptera - Cerambycidae) is a polyphagous invasive pest of both healthy and suppressed broadleaved trees in central Asia. CLB causes a great degree of damage in the countries of its origin. To understand this pest in a better way, a detailed review study has been conducted to gather all the valuable information related to the CLB. This review study covers the main aspects of the CLB, its morphology, ecology, distribution, damage, and control measures. The life span of the CLB is two years. It is mainly distributed in the central Asian countries and preferably damages broadleaved tree species, especially Juglans regia, Populus alba, P. euphratica, Acer spp, Salix alba, S. babylonica, Malus pumila, M. sylvestris, Platanus orientalis and Ulmus spp. Effective control and management practices include cutting, removing + burning of infested trees, cultivating high resilient tree species, use of biocontrol agents (i.e. fungus), and use of aluminium phosphide as a pesticide to significantly help control the CLB population. A CLB invasion in a new favourable habitat could impose a serious threat to broadleaf forests and plantations. Therefore, the keen monitoring and surveying of woodland management techniques are recommended. The strict monitoring and inspection of traded wood material at airports and seaports are highly recommended. There is a need to conduct multiple research studies to profoundly understand the CLB phenology, ecology, transportation mode, and damage severity to bridge the gap.

The human population is highly dependent on electricity, and to maintain the current progress of its use and for future requirements, it is necessary to look for ways to ensure sufficient energy. The aim of the article is to compare nuclear energy as a representative of non-renewable energy sources with photovoltaic energy as a representative of renewable energy sources (RES). The comparison is made in terms of the effect on agricultural land occupation, given that soil as a basic production factor is completely unique and that nuclear energy sources are often used as a backup for RES. We used a quantitative research method based on deduction using available real data to compare agricultural land occupation by these two energy sources. The results indicate that the effect of photovoltaic power plants on agricultural land occupation is 100 times greater than that of nuclear power plants. In terms of the effect on agricultural land occupation, the combination of solar and nuclear power plants seems to be appropriate. This combination partially eliminates the negative agricultural land occupation consequence of using solar power plants.

The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular identification of inoculation strain and investigated rhizobial isolates, obtained from nodules representing inoculated treatments, was performed based on 16S rDNA and enterobacterial repetitive intergenic consensus (ERIC) sequencing. Inoculation strain showed a significant effect on the investigated parameters in both soils. Higher nodule occupancy (45% vs. 18%), nodule number (111% vs. 5%), nodule dry weight (49% vs. 9%), shoot length (15% vs. 7%), root length (31% vs. 13%), shoot dry weight (34% vs. 11%), shoot nitrogen content (27% vs. 2%), and nodule nitrogen content (9% vs. 5%) was detected in soil without previously grown soybean and applied commercial inoculant. Soil had a significant effect on the shoot, root and nodule nitrogen content, while interaction of experimental factors significantly altered dry weight and nitrogen content of shoots, roots and nodules, as well as number of nodules. Nodulation parameters were significantly related with shoot dry weight, shoot and nodule nitrogen content. Symbiotic performance of inoculation strains in the field could be improved through co-selection for their competitiveness and effectiveness.

Pseudomonas aeruginosa is an infectious pathogenic bacteria infecting many different species of animals. Currently, it lacks a commercial vaccine. In this study, three monovalent DNA vaccines (poprL, poprF, and pflgE), three bivalent combination DNA vaccines (poprL+poprF, poprL+pflgE, poprF+pflgE), and a trivalent DNA vaccine (poprL+poprF+pflgE) were constructed. Consequently, we immunised chickens with these DNA vaccines and used inactivated vaccines as the positive controls. Then, the immune efficacy was evaluated through serum antibody detection, a lymphocyte proliferation assay, and cytokine concentration determination. Lastly, we assessed the protection rate through a challenge experiment. Following vaccination, the serum antibody levels induced using these DNA vaccines were different due to the different coating antigens. In the trivalent combination DNA vaccine group, we established that the lymphocyte proliferation (SI values), IFN-γ, IL-2, and IL-4 levels were significantly higher than those of the other six DNA vaccine groups and the inactivated vaccine group. However, the protection provided was slightly lower than that of the inactivated vaccine and higher than those of other DNA vaccines. The protection rate of poprL, poprF, pflgE, poprL+poprF, poprL+pflgE, poprF+pflgE, poprL+poprF+pflgE, and the inactivated vaccine were 50, 45, 60, 75, 80, 80, 90, and 95%, respectively. The results of this study indicated the trivalent DNA vaccine based on oprL, oprF and flgE genes represents a promising approach for the prevention of Pseudomonas aeruginosa infections.

To understand and promote the importance of business sustainability in agriculture as an important societal issue, one must use the circumstances to force modern-minded management to abandon the classical model of only complying with statutory obligations and to structure the knowledge to coordinate interdisciplinary approaches to ensuring sustainable innovation. The paper analyses the role of business sustainability in promoting sustainable innovation in agriculture. The study used a factor analysis applied to a sample of 183 companies (n₁ = 183) and qualitative research through focus groups (n₂ = 5). The main objective was to identify the factors influencing the innovated areas in relation to sustainability. The results of the factor analysis showed a six-factor solution: process approach, corporate social responsibility, quality management system, supply chain operation processes, demand for production, and employee performance.

In recent years, progress has been made towards incorporating the sociocultural perspective in the assessment of ecosystem services (ES) to identify the relevant services according to the different needs and perceptions of the populations consulted. New sociocultural assessment methods are being explored to respond to this emerging line of research, without a generally accepted alternative being found thus far. Aiming to contribute to this line of research, this article proposes a new methodological approach for the sociocultural assessment of ES. The new methodological approach is based on combining two different measurement procedures: a traditional Likert scale and the Kano model. The case of ES provided by forests will be considered for its development and application. With the application of the proposed methodology, the sensitivity and consistency of sociocultural assessments of ES would be improved, and the development of longitudinal analysis would be facilitated.

The objective of this study was to quantify the concentration of acrylamide in biscuits, bread, and cake obtained from industrial and local bakeries, classified based on their colour intensity as light, medium, or dark. The analysis was performed using gas chromatography-mass spectrometry (GC-MS). Sugar, moisture, and asparagine analyses were also carried out simultaneously. The results demonstrated that the acrylamide content in biscuits, bread, and cakes varied according to the colour (light 109.9 ± 7.95, 214.7 ± 27.40, and 128.6 ± 7.05; medium 176.3 ± 15.16, 387.0 ± 87.71, and 804.3 ± 17.16; and dark 407.6 ± 105.13, 555.8 ± 16.20, and 1 015.0 ± 83.68 µg·kg^–1, respectively). Statistically, significant differences were observed between acrylamide content and product colour density (P < 0.05). It can be concluded that the acrylamide content increases as the product colour intensity rises due to the increased baking temperature at which the reaction between reducing sugars and asparagine takes place, resulting in the formation of acrylamide. The results suggest that selecting commercial bakery products based on colour may be beneficial for reducing the daily intake of acrylamide by consumers. Therefore, it is recommended to avoid dark-coloured bakery products.

Grafting is the most important means of breeding peach, plum, apricot, and other fruit trees, and the selection of the rootstock is crucial to the quality of the grafting and the yield of the products. The traditional commonly used peach rootstock is susceptible to root-knot nematode infections, resulting in a decreased yield, while a variety of cherry plum rootstocks, Mirabolano 29C, is resistant to root-knot nematode. In this study, root-knot nematode infection experiments on seedlings of traditional peach rootstocks and Mirabolano 29C confirmed that Mirabolano 29C was indeed more resistant to root-knot nematodes. At the same time, we compared the roots of the root-knot nematode uninfected and infected Mirabolano 29C by transcriptome sequencing and found 3 176 differentially expressed genes. A further functional enrichment analysis of these genes found that the secondary metabolites, phenylpropane and flavonoids, may be responsible for the high resistance of Mirabolano 29C to root-knot nematodes. These results can provide a reference value for the disease resistance breeding of rootstocks.

A number of stimuli lead to what is termed "seasonal fatigue", where the only firm interconnection is the fact that this form of fatigue only occurs during a certain period of the season. The interior of the cabin and its effect on the machine operator is notable. The microclimate of the environment in which the operator works has a direct impact on his/her condition and, consequentially, the extent of his/her fatigue. The development of microclimate in a modern agricultural machine must be identified, and based on such findings, recommendation should be given regarding procedures for improving the current situation in agricultural technology. For testing, the New Holland T6.165 tractor was therefore selected. The individual measurements show that a certain drop of oxygen occurs in the cabin during the first half hour of driving. This leads to  an increase in CO₂, which is in turn caused by the higher ventilation of the given individuals present in the vehicle. To eliminate said drops in O₂, the use of oxygen concentrators may be recommended.

The objective of this study was to evaluate the effects of fat levels (200 g·kg^–1 meat and 500 g·kg^–1 meat) and types [lard, glycerolised lard (GL), and purified glycerolised lard (PGL)] on the quality and sensory characteristics of emulsion-type sausages. As observed, at the same type of fat, the low-fat sausage (200 g·kg^–1 meat) had a significantly higher L*-value (lightness) and lower cooking loss and total expressible fluid (P < 0.05) than the high-fat sausage (500 g·kg^–1 meat) and exhibited a denser and more homogeneous microstructure. Additionally, T₂₃ (relaxation time) of the low-fat sausage shifted toward a slower relaxation time, and higher A₂₃ (peak area) was found, which suggested the water mobility was restricted. However, at the same fat content, the low-GL and low-PGL sausages showed better textural properties and superior overall acceptability from sensory evaluation compared with the low-lard sausage (P < 0.05). Still, they have no significant differences (P > 0.05). Therefore, lard-based diacylglycerol could be effectively applied as a fat replacer in emulsion-type sausages with low-fat contents to produce healthier meat products.

Particle drift happens during herbicide application when droplets travel outside the intended site. Different nozzles produce various range of droplets, so they play a very important role in coverage and drift occasions. When nozzles produce small droplets, the potential for off-target movement is very high. Another important factor determining particle drift is the distance between crops. Wind velocity gives the energy to herbicide particles to move away from the target place. Therefore, a drift simulation of herbicide (mesotrione and rimsulfuron plus thifensulfuron-methyl mixture) was done in a wind tunnel, using different nozzles Extended Range (XR) and Turbo TeeJet Induction (TTI). The wind speed was set at 4.4 m/s, representing the least favourable conditions where applications are possible. In the wind tunnel, eight crops (cantaloupe, cotton, green bean, pumpkin, soybean, sunflower, wheat, and watermelon) were positioned at 4, 6, 9, and 12 m downwind distances from the nozzle, and drift was simulated. Following treatments, plants were returned to a greenhouse for 28 days, and biomass reduction was recorded. Artificial collectors (Mylar cards) and water sensitive cards were positioned alongside plants. According to obtained results, spraying with XR nozzle influences higher injuries than TTI nozzle. Tracer deposition was higher at all distances when XR nozzle was used. Accordingly, droplet numbers, covered area, Volume Median Diameter (VMD), and deposition were higher on water sensitive cards when spraying were done using XR nozzle. As a consequence, higher biomass reduction occurred using the XR nozzle. The most sensitive crops were cantaloupe, pumpkin and sunflower, while the most tolerant were soybean and wheat.

Alfalfa is an important leguminous plant, yield and quality depend on the growing environment, while effects of drought and heat stress on alfalfa leaves are unknown. This study was conducted to evaluate the effect of exogenous ascorbic acid (AsA) on photosynthesis, chlorophyll fluorescence, and xanthophyll cycle in alfalfa leaves subject to under drought and heat stress. The results suggest that drought and heat stress caused decreases in the net photosynthetic rate (P_n) in alfalfa leaves, but stomatal conductance (g_s), transpiration rate (T_r), and intercellular CO₂ concentration (c_i) were increased. The application of AsA could alleviate these changes to some extent. Besides, the decreased photosystem II (PSII) maximum photochemical efficiency (F_v/F_m) and violaxanthin (V) contents and significantly increased non-photochemical quenching (NPQ) levels. The increased NPQ corresponds to the de-epoxidation state (DPS) of xanthophyll pigments. In the AsA-pretreated alfalfa plants, the F_v/F_m and the NPQ were elevated, indicating that AsA could alleviate the adverse effects on photosynthesis induced by this stress. The violaxanthin de-epoxidase (VDE) enzyme activity was inhibited by drought and heat stress, and AsA significantly increased VDE enzymatic activity on the 2^nd and 8^th days. In summary, photoinhibition of PSII occurred in alfalfa leaves under drought and heat stress, resulting in decreased photosynthetic activity. Exogenous AsA can enhance the photosynthetic capacity of the plant, and enhance the drought and heat resistance of alfalfa.

Heat resistance of bacteria is a factor potentially limiting the production of safety foods. We focused on five Enterobacteriaceae strains related to cheese stretching and sub-pasteurisation experimental temperatures of 50–59 °C. Heat resistance was screened and obtained data were fitted to a classical log-linear model with D-values indicating highly heat-resistant strains used. For example in Klebsiella oxytoca S525, D(50)-value was 96.1 min and D(59)-value 5.1 min. In subsequent detailed measurements, the shape of inactivation curves was sigmoid with defined lag, log-linear and stationary phase. We suggest calculating refined D-values (D^r-values) using only data obtained in log-linear phases, namely D^r(temperature; lag phase). In K. oxytoca S525, the obtained results were: D^r(50; 80.9) = 61.7 min, D^r(53; 12.4) = 36.8 min, D^r(56; < 10) = 10.6 min, and D^r(59; < 3) = 4.3 min. The research of particular inactivation phases can provide interesting findings both in science and industrial practice, especially concerning the passage or persistence of hazardous strains in food processing plants.

Identification of the species for dendrochronological studies is of great relevance to understand various aspects of climate change. However, in the northwestern Himalayan region, dendroclimatological investigations are confined to conifer species, with broadleaved species being disregarded. Thus, the present study was conducted to assess the growth response of seven multipurpose tree species (MPTs), namely Bauhinia variegata, Celtis australis, Grewia optiva, Paulownia fortunei, Toona ciliata, Ulmus villosa and Melia composita to local climate variables, viz. temperature as well as rainfall (seasonal, monthly, average) and CO2 level by evaluating the climatic signal in tree ring chronologies at Solan district, India (altitude 1 250 m) in the mid-hills of the northwestern Himalayas. The results indicated that only the maximum, rainy season temperature and CO2 level varied significantly (P < 0.05) between 1991 and 2017. Only G. optiva exhibited a significant (P < 0.05) tendency toward increased growth. C. australis has a remarkable negative correlation with temperature variables, viz. average, maximum, spring season, March temperature, whereas T. ciliata exhibits a positive correlation with temperature variables, such as rainy season, average and April temperature. Similarly, winter, total and December rainfall have a profound effect on P. fortunei, while March rainfall adversely affected the growth of B. variegata. On the other hand, G. optiva demonstrated sensitivity to both temperature (February and May) and rainfall variables (winter, February and May). U. villosa recorded a positive correlation with rainfall (autumn and October rainfall) but a negative correlation with temperature variables (maximum and April temperature). Elevated CO2 levels affected only two species (G. optiva, M. composita) out of the seven selected species. Our findings will contribute to a better understanding of the climate growth relationships of investigated tree species, as a result, to more accurate projections of the effects of climate change on these MPTs and directing future studies.

The intensification of Alopecurus myosuroides Huds. (black-grass) is becoming a major problem due to its growing resistance to a broad spectrum of acetolactate synthase (ALS)-inhibiting herbicides. Hence, the present study was conducted to evaluate the resistance level of a black-grass population to iodosulfuron plus mesosulfuron and to identify the underlying resistance mechanism. Dose-response studies revealed that the resistance population is 22 times less sensitive to iodosulfuron plus mesosulfuron than the susceptible population. The probable resistance mechanism identified was the target-site substitution of proline (Pro) by threonine (Thr) at the 197^th position of the ALS enzyme. Furthermore, whole plant response bioassay experiments demonstrated that this population is also resistant to pinoxaden, chlorotoluron, diflufenican plus pendimethalin plus chlorotoluron, fenoxaprop and flufenacet plus diflufenican. In summary, the current findings recommend using alternative herbicides in integrated weed management to interrupt the possible evolution of herbicide resistance in these species.

The aim of this study was to determine the effect of a granular poultry manure fertiliser on the yield and the quality of white cabbage (Brassica oleracea var. capitata), and to determine the effect on the soil agrochemical properties. The effect of the granular poultry manure fertiliser and mineral fertiliser (applied separately and combined at different times – in early spring and in autumn) on the cabbage yields was determined. Investigations were carried out in the fields of the Institute of Horticulture, the Lithuanian Research Centre for Agriculture and Forestry. The object of the research – white cabbage ‘Socrates’ H. The highest white cabbage marketable yield (80.5 t/ha) and the highest amount of vitamin C (7.80 mg per 100 g of products) was obtained by applying the granular poultry manure fertiliser in the autumn and the mineral fertiliser in the spring, where the granular poultry manure fertiliser applied in the early spring and the granular poultry manure fertiliser applied in the autumn with the mineral fertiliser applied in spring retained a higher content of humus (1.82–1.94%), organic carbon (1.06–1.12%) and total nitrogen (0.099–0.147%). The mineral nitrogen residue in the soil decreased when the cabbage was fertilised in the spring with the mineral fertiliser and when fertilised in the spring with the mineral fertiliser in combination with the granular poultry manure fertiliser by 8.1–10.8 kg/ha. The minimum content of nitrates in the heads of the white cabbage was found after using the granular poultry manure fertiliser in the autumn.

A 9-month-old Holstein heifer with a history of severely poor growth presented with diarrhoea. On physical examination, a metallic pinging sound was heard on the simultaneous percussion and auscultation of the left trunk. On the cranial abdominal radiography, the contour of a gas-filled balloon-like abomasum wall was delineated, which elevated to the dorsal abdomen. Radiopaque sand at the bottom of the abomasum had been pulled up caudodorsally by the gas-filled abomasum. After surgery, the gas-filled balloon-like appearance of the abomasum wall disappeared and the radiopaque sand was located in the normal position. To our knowledge, no reports on a displaced abomasum on plain radiographs are available. The radiographic findings described herein are characteristic imaging findings of a displaced abomasum. Abdominal radiography could be a new option as an auxiliary diagnostic approach for a displaced abomasum.

The case study aims to describe the nephrotic syndrome (NS) in a castrated 3-year-old male Cocker Spaniel dog. The patient arrived at the hospital with a loss of appetite and weakness. Skin oedema with ascites was observed along with hypoalbuminaemia, hypoproteinaemia, hyperlipidaemia, hypercholesterolaemia, and proteinuria (urine protein to creatinine ratio = 22.4). Based on these findings, the patient was diagnosed with NS, although a renal biopsy was not conducted. Prednisolone (1 mg/kg, p.o. q12 h) and mycophenolate mofetil (10 mg/kg, p.o. q12 h) were prescribed as the immunosuppressive drugs, and previously cryopreserved allogeneic adipose tissue-derived mesenchymal stem cells (2 × 10⁷ cells/kg) were injected intravenously. After several weeks of treatment, the patient recovered from NS. This is the first case report on immunosuppressive drugs and allogeneic mesenchymal stem cells being used to treat a dog with NS.

The weevil, Myllocerus fotedari Ahmad, 1974 (Coleoptera: Curculionidae) is widely recognised as one of the major walnut pests. Fungal pathogens have shown great potential for the management of some pest species. In the present study, the efficacy of three entomopathogenic fungi, namely Beauveria bassiana (Balsamo) Vuillemin, Metarhizium anisopliae sensu lato (Metchnikoff) Sorokin and Lecanicillium lecanii (Zimmerman) Zare and Gams were evaluated against the weevil, M. fotedari, under laboratory conditions. Each fungal suspension contained 1.0 × 109 spores of fungi in 1 mL. The experiment comprised of five treatments along with control (distilled water) and three replicates. An insecticide – chloropyrifos 20% emulsifiable concentrate (EC), was also used as positive control in the experiment. The experimental results revealed that the weevil, M. fotedari, showed mortality due to both virulence of entomopathogens and insecticide. The mortality caused by fungi varied from two days after treatment to eight days after treatment. However, the highest mortality of 100% was recorded for B. bassiana, followed by 85.1% for M. anisopliae after eight days of treatment. The efficacy of L. lecani was the lowest, leading to only 51.8% mortality, and was found significantly less virulent compared to the other two used entomopathogens. The present study is an attempt to use entomopathogens to control M. fotedari over conventional chemical insecticides.

The susceptibility of 14 pear cultivars to the bacterium Erwinia amylovora, the causal agent of fire blight disease, was evaluated using three different methods of in vivo inoculation – the leaf inoculation method on detached shoots of pear cultivars in a climate chamber, and the terminal shoot and blossom inoculation methods on potted trees of the same pear cultivars in a net house. The results of the artificial inoculations of the European and Asian pear cultivars were compared using the susceptibility score scale, as well as the ANOVA and post hoc methods. The differences among the pear cultivar susceptibility increased during the evaluation periods in the given year, but there were no significant differences between the annual evaluation in 2020 and 2021. The results of the pear cultivar susceptibility after inoculation of the detached shoots differed significantly from the results of the terminal shoot and blossom inoculation. The detached shoot susceptibility under pathogen-favourable conditions in the climate chamber was lower than the terminal shoot and blossom susceptibility in the net house for most pear cultivars. In both years, the highest values of the terminal shoot and blossom susceptibility were coincidently calculated for the Ananaska česká European old cultivar and the Nijissejkii Asian cultivar.

In 2021, measurements were done at two international provenance research trials for silver fir originating from the same series of experiments. The investigation was carried out in the location Vítkov and Úsov, where both trials were established. Biometric data (tree height, diameter at breast height) were measured and qualitative traits (stem shape, occurrence of stem forking, stem damage, bark pattern, and defoliation) were assessed during the early mature stage of the experiment. Overbark stem volume and per-hectare standing volume were also calculated. Sixty-five provenances of domestic and foreign origin were evaluated in both trials. Although the results do not indicate unequivocally the most suitable or most productive provenance in the trials, provenances of Czech origin including the ones originating from the surrounding natural forest areas perform consistently better than the average. The least productive provenances, on the other hand, were those from parts of Bulgaria, Austria, and especially Italy, which achieved the poorest results even in stem shape. In Czech conditions, therefore, Italian fir provenances have not proved so successful as they have in the United Kingdom.

The excessive use of chemicals in plant production, including mineral fertilizers has a harmful effect on the morpho- and physiological state of strawberry plants, their yielding, and the physicochemical properties of the fruit and soil. Increasing the effectiveness of beneficial microorganisms, i.e. filamentous fungi, arbuscular mycorrhizal fungi (AMF) and bacteria, is an essential method of reducing the amount of fertilizers used to fertilize the soil. The aim of the study was to assess the effect of beneficial soil microorganisms (filamentous fungi and bacteria) applied alone or together with mineral fertilizers on the morpho- and physiological state of ‘Marmolada’ strawberry plants, their yielding, and the physico-chemical properties of the fruit. The experiment included the application of fungi (Aspergillus niger and Purpureocillium lilacinum), bacteria (Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa) alone or together with mineral fertilizers (Polifoska 6, Urea, Super Fos Dar 40). The study investigated the number of inflorescences and flowers, the intensity of the green colour of leaves, fruit yield and weight (g and %), pH, extract (°Brix), titratable acidity (g/100 g), fruit firmness (N), as well as the concentrations of macro-and microelements in the leaves of the plants. The obtained results depend on the type of fertilizer and its concentration. Strains of filamentous fungi or bacterial strains only (without fertilizer), increase the fruit yield by 60 and 35%, respectively. Reduced to 60% doses of Polifoska 6, Urea, and Super Fos Dar 40 combined with beneficial soil microorganisms influence the strawberry fruit yield more beneficially than the fertilizers applied in standard doses (100%). The mineral fertilizers enriched with soil beneficial fungi and bacteria increase the mean fruit weight by 25–30%. Both Super Fos Dar 40 enriched with bacterial strains and Polifoska 6 at 100% positively affect the physical and chemical properties of strawberry fruits. The application of reduced to 60% doses of Fos Dar 40 and Polifoska 6 enriched with beneficial bacterial strains increase the nitrogen and potassium contents in the leaves, respectively. Urea and Super Fos Dar 40 at 100% enriched with beneficial bacteria positively affect magnesium content in the leaves. Application of Polifoska 6 and NPK enriched with bacteria beneficially influences the iron content in the leaves. The calcium content in strawberry leaves decreases due to fertilization.

The aim of the study was to compare the efficacy of the intranasal and parenteral administration of azaperone in order to achieve pig sedation. A total of 32 weaned piglets divided into 4 groups (8 piglets in each group) were used. Group A was injected intramuscularly (i.m.) with azaperone (Stresnil®, 40 mg/ml inj.; Elanco Animal Health) at a dose of 2 mg/kg of body weight (b.w.). Group B received a dose of 2 mg/kg b.w. of azaperone intranasally. Group C was given azaperone intranasally at a dose of 4 mg/kg b.w. Group D was given 1 ml of saline intranasally and served as the control group. The response to the defined stimulus (a blunt blow of a metal rod into a metal edge of a pen), the degree of salivation, movement level, body temperature and serum azaperone concentration were included in the trial. We found that in order to induce an adequate level of sedation comparable to the standard method of application, i.e., 2 mg/kg b.w. i.m., the intranasal administration of azaperone at a dose of 4 mg/kg body weight is required.