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Results 1471 to 1500 of 4598:

Genome-wide characterisation, evolution and expression analysis of the leucine-rich repeat receptor-like kinase (LRR-RLK) gene family in cucumbersOriginal Paper

Jia Yu, Bo Zhang, Sisi Liu, Wei Guo, Yifan Gao, Hongyan Sun

Plant Protect. Sci., 2022, 58(2):125-138 | DOI: 10.17221/131/2021-PPS

The leucine-rich repeat receptor-like kinases (LRR-RLKs) compose a large gene family in plant genomes and implement essential functions in diverse plant physiology progress, including defence against pathogens. However, a systematic analysis of LRR-RLKs has not been accomplished in the economically important cucumber. 189 LRR-RLK genes were identified in the cucumber genome and further divided into 22 subgroups based on the sequence similarities in this study. A total of 31 segmental duplication events and 15 tandem duplication events were present in the genome, indicating that the two duplications were the main driving forces for the expansion of the LRR-RLK family in the cucumber. The expression profile analysis revealed that most of the CsLRR-RLKs were upregulated during a downy mildew infection, and resistant cucumbers comprised more upregulated CsLRR-RLKs than the sensitive lines. Taken together, our results provided information on the LRR-RLK gene family in the cucumber and contributed valuable information for the further research of CsLRR-RLKs.

Effects of freeze-thaw on soil properties and water erosionReview

Baoyang Sun, Feipeng Ren, Wenfeng Ding, Guanhua Zhang, Jinquan Huang, Jianming Li, Lei Zhang

Soil & Water Res., 2021, 16(4):205-216 | DOI: 10.17221/143/2020-SWR

Freeze-thaw erosion occurs primarily at high latitudes and altitudes. Temperature controlled freeze-thaw events dislodge soil particles and serve as a catalyst for erosion. This review paper provided an overview of the effects of freeze-thaw on soil properties and water erosion. The process of freeze-thaw cycles results in temporary and inconsistent changes in the soil moisture, and affects the soil's mechanical, physical and chemical properties, such as the soil moisture content, porosity, bulk density, aggregates stability, shear strength and organic matter content and so on. The variation trend and range of the soil properties were related to the soil texture, water content and freeze-thaw degree. Furthermore, the soil erosion was affected by the freeze-thaw processes, as thawing and water erosion reinforce each other. However, research of different experimental conditions on indoor simulations have numerous limitations compared with field experiments. The use of indoor and field experiments to further reveal the freeze-thaw effect on the soil erosion would facilitate improved forecasting.

Glycoalkaloids in leaves and potato tubers depending on herbicide application with biostimulantsOriginal Paper

Krystyna Zarzecka, Marek Guga³a, Iwona Mystkowska, Anna Sikorska, £ukasz Domañski

Plant Soil Environ., 2022, 68(4):180-185 | DOI: 10.17221/52/2022-PSE

The aim of the study was to determine the influence of herbicide application with biostimulants on total glycoalkaloid (TGA) content in potato leaves and tubers. The study was based on a two-factor field experiment established in a split-plot arrangement in three replications. The factors were as follows: factor I - two cultivars of edible potato - Oberon and Malaga; factor II - five methods of using herbicide and biostimulants: 1. control object; 2. herbicide clomazone + metribuzin (Avatar 293 ZC); 3. herbicide clomazone + metribuzin and biostimulant PlonoStart; 4. herbicide clomazone + metribuzin and biostimulant Aminoplant; 5. herbicide clomazone + metribuzin and biostimulant Agro-Sorb Folium. The herbicide clomazone + metribuzin significantly increased the concentration of glycoalkaloids in potato leaves, and the herbicide used together with the biostimulant Aminoplant significantly reduced the content of TGA in potato leaves compared to the control. The herbicide whether applied with the biostimulants (PlonoStart, Aminoplant, Agro-Sorb Folium) contributed to a reduction in the concentration of glycoalkaloids in tubers compared to the control. Leaves and tubers of the cultivar Malaga were characterised by a significantly lower TGA content than the cultivar Oberon.

Prediction of the rhodinol content in Java citronella oil using NIR spectroscopy in the initial stage developing a spectral smart sensor system - Case reportShort Communication

Dedi Wahyudi, Erliza Noor, Dwi Setyaningsih, Taufik Djatna, Irmansyah Irmansyah

Res. Agr. Eng., 2022, 68(4):216-222 | DOI: 10.17221/87/2021-RAE

The rhodinol content is an essential component in determining the citronella oil qualities. This study aimed to develop a model calibrated to predict the rhodinol content in citronella oil using near-infrared (NIR) spectroscopy. This research is the initial stage in developing a spectral smart sensor system that predicts the rhodinol content of citronella oil in the distillation and fractionating process. Citronella oil samples were scanned by NIRFlex liquid N-500 with a wavelength of 1 000-2 500 nm having an absorbance value (log 1/T). The accuracy of the prediction was achieved using the partial least square (PLS) model. Based on the NIR spectrum at a peak of around 1 620 nm, the rhodinol content in the citronella oil was estimated. The finest model to predict the rhodinol content was y = 0.9874x + 15.6439 with a standard error of the calibration set (SEC) = 2.78%, a standard error of the prediction set (SEP) = 2.88%, a ratio of the performance to the deviation (RPD) = 9.23, a coefficient of variation (CV) = 16.81%, and the correlation coefficient (r) = 0.99. The NIR and PLS models are possible to use for the initial stage in developing a spectral smart sensor system to determine the rhodinol content of citronella oils.

Clinical and radiographic evaluation of minimally invasive plate osteosynthesis (MIPO) in dogs with tibial fracturesOriginal Paper

C Istim, M Arican

Vet Med - Czech, 2022, 67(6):316-322 | DOI: 10.17221/42/2021-VETMED

The aim of this study was a clinical and radiographic evaluation of the minimally invasive plate osteosynthesis (MIPO) method for treatment in dogs with tibial fractures. Ten dogs of different breeds, ages, and sex with tibial fractures were used as the subjects. A medial approach to the tibia was used in all the cases. The tibial tuberosity was palpated proximally and a short incision was made along the midline. After reduction of the fracture, proximal and distal insertion incisions were made to approach the tibia. The mean times for the MIPO operations were 21 minutes. The patients were able to stand and walk postoperatively on the first day and recovery was rapid for all of the dogs. The fracture healing was completed on day 45 based on the radiographic evaluations of these cases. Dogs with tibia fractures treated by MIPO healed rapidly without any complications (90%). Correct fracture selection is important for the use of MIPO to be successful. Further studies with a larger group of dogs should be considered to compare the efficacy of MIPO and other fixation techniques and for the long-term evaluation of the repaired tibial fractures.

Food security in a food self-sufficient economy: A review of China's ongoing transition to a zero hunger stateReview

Vasilii Erokhin, Gao Tianming, Luminita Chivu, Jean Vasile Andrei

Agric. Econ. - Czech, 2022, 68(12):476-487 | DOI: 10.17221/278/2022-AGRICECON

In a contemporary globalised world, assuring food security in the conditions of developing a food self-sufficient economic structure represents not only a challenge in terms of the changing agricultural paradigm, but also an important instrument in assuring, in a long-term manner, societal resilience. However, while achieving self-sufficiency in food, China still faces challenges in terms of establishing sustainable food security and transitioning to a zero hunger state. Rapid economic growth and urbanisation have resulted in shifting food consumption patterns from crops towards more nutritious meat and dairy products and higher-qualitative imported foods. In this study, the current state of food security and production and trade in food and agricultural products in China during 2000-2020 have been analysed and an overview of the strategic directions of the domestic sustainable development, food security, and zero hunger policies are highlighted. The main aim of the study is dedicated to identifying the critical shortcomings and gaps in combating hunger and food insecurity in China and proposing policy recommendations regarding improving the stability of the food supply in the country and deigning new possible strategies to achieve it. The results highlight the major trends and shifts of the food security paradigm in the process of developing a food self-sufficient economy.

The effect of defoliation on the 3-isobutyl-2-methoxypyrazine biosynthesis in wine grapesShort Communication

Yujuan Lei, Zhansheng Ma, Pengbao Shi, Yuxia Sun, Ping Wang, Xueqiang Guan

Hort. Sci. (Prague), 2022, 49(2):117-120 | DOI: 10.17221/70/2020-HORTSCI

Field studies were conducted on Vitis vinifera cv. 'Cabernet Sauvignon' to evaluate the effects of the defoliation time (pre- and post-flowering and veraison) and severity on the 3-isobutyl-2-methoxypyrazine (IBMP) concentration and expression levels of the key related genes in grape berries. The IBMP concentration in the grapes decreased significantly at harvest after all the defoliation treatments. The earlier and heavier the defoliation treatment, the lower the IBMP concentration. The relative expression level of VvOMT3 in the berry skin was closely positively correlated with the IBMP accumulation in all the treatments. Early defoliation coupled with a 1-week delay in the harvest time may be an effective management strategy to control the IBMP concentration in grapes.

Preferences in sweet cherry fruits among consumers in Serbia and Bosnia and HerzegovinaOriginal Paper

Gorica Paunoviæ, Ðurað Hajder, Aleksandra Koriæanac, Boris Pa¹aliæ, Ivan Gli¹iæ, Tatjana Jovanoviæ-Cvetkoviæ, Miljan Cvetkoviæ

Hort. Sci. (Prague), 2022, 49(4):189-196 | DOI: 10.17221/8/2022-HORTSCI

The introduction of new varieties in fruit production should consider consumer attitudes regarding the appearance and taste of the fruits. In order to evaluate the consumer attitudes in Serbia and Bosnia & Herzegovina (B&H), on the most important characteristics of cherry fruits, an online survey was conducted with 402 respondents. Participation in the survey was taken by 53.23% of respondents from Serbia and 46.77% from B&H, most of which were women (54.23%). Consumers expressed their views on the most important external (fruit colour, fruit shape, fruit size, presence of a stalk on the fruit, length of the stalk and the presence of damage to the fruit) and internal (fruit taste, fruit firmness) characteristics of the fruit. According to the results, the attitude toward the fruit size, the presence of a stalk and the stalk length, as well as fruit firmness was dependent upon respondents' residence. Both male and female respondents had similar attitudes toward the cherry characteristics except for the fruit taste and the most important cherry characteristics. The age of a respondent influenced the attitude toward the fruit size and the presence of a stalk, as well as the fruit firmness.

Development and evaluation of a combined roaster expeller for castor seeds for biodiesel productionOriginal Paper

Rasheed Amao Busari, Joshau Olarnrewaju Olaoye, Emmanuel Segun Adebayo, Adeshina Fadeyibi

Res. Agr. Eng., 2022, 68(4):169-179 | DOI: 10.17221/83/2020-RAE

A combined roaster and oil expeller was developed with locally available and accessible materials and the efficiency of the machine was evaluated. The obtained result shows that the efficiency of the machine is a function of the roasting temperature, the roasting duration, the moisture contents of the processed seeds and the machine feeding rate. The expeller is movable, simple in design for local fabrication, is easy to operate, requires few repairs and little maintenance and is cost effective. It is powered using a gear reduction electric motor of 5.5 Hp, the expeller has an average oil yield of 25.77% and an extraction efficiency of 70.26% and is designed to work for 8 hours per day of operation. The shaft diameter was designed to be 30 mm, while the roaster heater capacity was 2.8 kW. The designed machine is good for castor oil expression for both small- and medium-scale processing among rural and urban communities.

Understanding psychological factors influencing ecosystem conservation behaviours of forest professionalsOriginal Paper

Norris Igbinosa Erhabor

J. For. Sci., 2022, 68(6):220-227 | DOI: 10.17221/39/2022-JFS

This study was embarked upon to assess forest guards' Knowledge, Attitude and Practice (KAP) behavioural change sequences in Nigeria lowland forest reserves using a structural equation modelling approach. Two research questions were raised and corresponding hypotheses were tested. From the data analysed, it was revealed that both knowledge and attitude variables significantly predicted the behaviour of the forest guards' towards the management of the forest and its resources. Although the attitude variable on its own did not significantly predict the forest guards' behaviours (t = 1.401, P > 0.05), it can be stated that the knowledge of the forest guards towards the forest is a better predictor to their environmental behaviours and their behavioural change sequence (AKP) is inclined towards developing affinity to the forest. Hence seminar and symposiums should be organized for all forest guards with the objectives of having better efforts and persistence, preparation for action, better visualization of success, reduce stressors, and fewer intrusive negative thoughts in managing the forest and its resources. This will help boost their affinity to the forest and self-efficacy as the study revealed that the Attitude-Knowledge-Practice (AKP) sequence explains better the behaviour of the forest guards in protecting the forest reserves.

Assessment of chemical contaminants in fresh and packaged tender coconut (Cocos nucifera) waterShort Communication

Padmaja Rambabu Jonnalagadda, Srujana Medithi, Summaiya Lari, Kalyan Chinthanuri, Yogeswar Dayal Kasa, Raju Nagaraju, Janardhan Vanka, Venkaiah Kodali

Czech J. Food Sci., 2022, 40(2):154-162 | DOI: 10.17221/200/2021-CJFS

Pesticide residues and heavy metals were analysed in both fresh tender coconut water (FTCW) (n = 161) and packaged tender coconut water (PTCW) (n = 126) samples collected from three southern states of India [Andhra Pradesh (AP), Kerala (KL), and Tamil Nadu (TN)]. A method validated in the laboratory using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for pesticide residues, while heavy metals were analysed using a validated method of inductively coupled plasma-optical emission spectrometry (ICP-OES). Significant differences in heavy metal concentrations were assessed using analysis of variance (ANOVA) and post hoc test (between different varieties collected 'within' and 'among' states). FTCW samples [n = 9 (6%)] collected from TN showed Monocrotophos and Malathion residues in the range of 1.0 µg L-1 to 51.6 µg L-1 and 0.5 µg L-1 to 0.6 µg L-1, respectively, while they were detected in n = 5 (4%) of the PTCW samples at a range of 0.90 µg L-1 and 0.82 µg L-1 to 1.56 µg L-1. Heavy metals such as cadmium (Cd), chromium (Cr), lead (Pb), and stannum (Sn) were detected in different varieties collected from all three states. Some of the PTCW samples also contained traces of Cd, Cr, cobalt (Co), and Pb. Arsenic (As) was found in one sample from KL, while none of the samples was contaminated with mercury (Hg). The present study accentuates the need for fixing standards for the pesticide residues in coconut water.

Effect of toys on behaviour and body weight of weaned pigs after mixingOriginal Paper

Hyun-Su Hwang, Jae-Kang Lee, Tae-Kyung Eom, Dong-Ho Lee, Shin-Jae Rhim

Czech J. Anim. Sci., 2021, 66(8):323-330 | DOI: 10.17221/22/2021-CJAS

This study was conducted to evaluate the effects of a toy on the behaviour and body weight of weaned pigs after mixing. Two groups (192 pigs in total, control: without toy; treatment: with toy placed on the floor) of weaned pigs housed in pens (1.8 m × 1.4 m, 4 pigs/pen) were This study was conducted to evaluate the effects of a toy on the behaviour and body weight of weaned pigs after mixing. Two were observed with the aid of video technology for nine consecutive hours in the early (days 1 and 2) and late (days 38 and 39) period after mixing. Bodyweight of pigs at the end of the study (Mann-Whitney U-test, Z = -2.46, P = 0.01) and the average daily gain (Z = -2.75, P < 0.01) were 10% higher in the treatment group than in the control group. The presence of the toy in the pen influenced the pig behaviours. In pens enriched with toys, there was an increase in feeding behaviour and a decrease in agonistic and belly nosing behaviours. Toy supply reduced negative social behaviours and thereby improved the bodyweight of weaned pigs. The results of this study suggest that toys used as environmental enrichment are useful for enhancing pig welfare and effective for farmers.

Responses of nitrogen accumulation and translocation in five cytoplasmic hybrid rice cultivarsOriginal Paper

Noor Muhammad, Yating Zheng, Farhan Nabi, Guotao Yang, Sumbal Sajid, Abdul Hakeem, Xuechun Wang, Youlin Peng, Zartasha Khan, Yungao Hu

Plant Soil Environ., 2022, 68(2):73-81 | DOI: 10.17221/354/2021-PSE

To study the difference among cytoplasm at the different nitrogen conditions, a research experiment was conducted using five different cytoplasmic male sterile (CMS) hybrid rice with nitrogen levels at N0, N1, N2, and N3; the nitrogen application rates were 0, 90, 180, and 270 kg/ha, from 2018 to 2019. Results showed that among tested cultivars of CMS hybrid rice, JW (J803A × Chenghui727) showed the highest yield in both years for the low nitrogen and high nitrogen treatments. The dry matter accumulation and translation of JW type in nutritive organs were higher than that of others during the low nitrogen level (N1). We concluded that the nutrient translocation within plants organs and dry biomass accumulation were highly dependent on CMS type and nitrogen application. This research indicates that selecting a rice cultivar with greater efficiency of nitrogen is favourable for raising the number of grains per panicle, grain yield, and nitrogen use efficiency. JW cytoplasm displayed great efficiency in low nitrogen, which is a potential cytoplasmic resource.

Pyrolysis of maize cob at different temperatures for biochar production: Proximate, ultimate and spectroscopic characterisationOriginal Paper

Timothy Adekanye, Oluwasogo Dada, Jegede Kolapo

Res. Agr. Eng., 2022, 68(1):27-34 | DOI: 10.17221/106/2020-RAE

Adopting the concept of the waste to wealth approach, agricultural waste from maize cob could be transformed into a renewable form of energy through thermo-chemical methods of treating the biomass. This method can be utilised for biochar production. The utilisation of biochar has several significant applications. These applications include the enhancement of the soil through amendment, stimulation of crop production by a variety nutrient inputs in the soil, etc. In this research work, a biochar was obtained through a slow pyrolysis process of maize cob waste. This experiment was carried out using a small-scale muffle furnace and subjecting the feedstock to heating at different temperatures (300, 400, 500 °C). The biochar was produced and characterised by a proximate analysis, scan electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, while the surface area was determined by Saer's method. The effect of the temperature on the yield of the biochar was investigated. The results show that the biochar yield decreases with an increasing temperature for the maize cob biochar at 300, 400 and 500 °C. The results of the physiochemical properties showed that the temperature has a great impact on the physicochemical properties of the biochar. The biochar produced at 300 °C has the highest fixed carbon content of 60.5%. The largest surface area was (281.8 m2.g-1) at 500 °C.

Clinical cases of zoonotic Cryptosporidium parvum (subtype IIdA15G1) infections in Korean goatsCase Report

J Kim, K Lee, SG Roh, HY Kim, JW Kim, BJ So, EJ Choi

Vet Med - Czech, 2022, 67(3):156-162 | DOI: 10.17221/212/2020-VETMED

Cryptosporidium parvum is an enteric protozoan, which causes severe diarrhoea in a wide range of vertebrate hosts including ruminants and humans. C. parvum infections are responsible for immense economic losses to the livestock industry; furthermore, the zoonotic spread of the infection may lead to mortality in immunosuppressed humans. In the present study, we report two cases of severe cryptosporidiosis in goat kids in the Republic of Korea. Both cases were afflicted with severe diarrhoea upon presentation; the histopathological examinations revealed atrophied and fused intestinal villi and numerous circular basophilic organisms in the jejunum and ileum. Both cases were diagnosed with cryptosporidiosis based on the results of the histopathological analysis, amplification of the C. parvum gp60 gene, modified Ziehl-Neelsen staining, and C. parvum antigen ELISA. According to the phylogenetic analysis using the C. parvum gp60 gene for the genetic subtypes, the C. parvum isolates were identified as subtype IIdA15G1 with zoonotic potential. This is the first pathological report of caprine cryptosporidiosis induced by C. parvum subtype IId in the Republic of Korea. Considering the clinical manifestations associated with the pathological lesions and the zoonotic significance of these findings, the continuous monitoring and prevention of C. parvum infections in goats are essential for minimising the economic losses in ruminant farms and in maintaining public health safety standards.

Impact of fertilisers on five turfgrass mixtures for football pitches under natural conditionsOriginal Paper

Barbara Zanelli, Matej Vidrih, Tanja Bohinc, Stanislav Trdan

Hort. Sci. (Prague), 2021, 48(4):190-204 | DOI: 10.17221/160/2020-HORTSCI

For 2 years (2019-2020), a field experiment to test the activities of different fertilisation schemes on the yield, colour and health status of five different grass mixtures for football pitches was conducted. Two grass mixtures were composed of different varieties of perennial ryegrass, one mixture was composed of varieties of perennial ryegrass and Kentucky bluegrass, one mixture consisted of the species Lolium perenne, Festuca rubra, Festuca arundinacea and Poa pratensis, and the seeds of only one variety of perennial ryegrass were sowed in one plot. Three different fertilisation schemes were included in the experiment. The first scheme (A) contained an inorganic fertiliser with added soil improvers, the second scheme (B) included an organic fertiliser with added soil improvers and the third scheme (C) was composed of an inorganic fertiliser. According to this study, the grass yield largely depends on the soil temperature, amount of precipitation and soil water content, and the occurrence of the fungus Laetisaria fuciformis is influenced by the fertilisation scheme, as the % infection with the fungus was highest when organic fertilisers with low % nitrogen was used. The selection of fertilisers is seen as an important factor for the turfgrass colour.

Functional profile of carob (Ceratonia siliqua L.) beans and pod pulp originated from the Republic of MoldovaOriginal Paper

Tatiana Capcanari, Aurica Chirsanova, Oxana Radu, Eugenia Covaliov, Violina Popovici, Rodica Siminiuc

Czech J. Food Sci., 2022, 40(6):465-473 | DOI: 10.17221/139/2022-CJFS

This study provides the first insight into the biologically active potential (total phenolic compounds, flavonoids, tannins and antioxidant activity) of Moldavian сarob beans and pod pulp in comparison with carob grown in Algeria, Spain, and Italy. The results showed that the samples of Moldavian carob contain significant amounts (P ≤ 0.05) of biologically active compounds, the content of some of these compounds is far exceeding that of сarob from the above-mentioned regions. Thus, the total content of phenolic compounds in Moldavian carob samples is 1.4 times higher, of flavonoids 1.9 times higher compared to the imported ones. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant activity of Moldavian carob samples proved to be about 10-12% higher than the antioxidant activity of samples from other regions. It has been proved that Moldavian carob pod pulp and beans have a high biologically active potential making them possible ingredients for functional food products.

Contribution of the forest sector to the Uruguayan economy: A first approach with National AccountsShort Communication

Virginia Morales Olmos, Ernesto Pienika

J. For. Sci., 2022, 68(3):116-119 | DOI: 10.17221/149/2021-JFS

The forest sector development in Uruguay has been based on planted forest areas, mainly with eucalyptus and pine. The Parliament passed the Forestry Law in 1987 and since then the sector has been growing rapidly. The objective of the study is to analyse the contribution of the forest sector in the Uruguayan economy, its evolution between 1997 and 2018 and its distribution in recent years. The research used information from the new National Accounts released by the Central Bank of Uruguay, and, in this framework, the forest sector is studied for the main macroeconomic variables and production. The results show that in the period 1997-2018, while the Uruguayan economy grew 2.6% in constant terms, on average, the forest sector grew 7.6% on average, with peaks in 2007-2008 and 2013-2015. In 2016, the forest production was allocated to exports (46.76%), which surpassed intermediate use (43.05%) after initial operations of the second pulp mill in the country. Furthermore, forest exports represented 10.5% of the country's total exports of goods and services, showing an exporter profile.

Characterisation of the little cherry virus 1 isolate infecting almonds in the Czech Republic Original Paper

Dana ©afáøová, Jakub Lemberk, Milan Navrátil

Plant Protect. Sci., 2022, 58(4):292-297 | DOI: 10.17221/63/2022-PPS

Little cherry virus 1 (LChV1) of the genus Velarivirus is an important pathogen associated with the little cherry disease. It infects various species of the genus Prunus, mainly the sweet cherry and the sour cherry. Recently, plums and apricots have also been reported as natural hosts. In the present work, the LChV1 isolate causing chlorotic mosaics in almonds is characterised. The nearly complete (+)ssRNA genome sequence of the Alm138 isolate, obtained by Sanger sequencing, is 16 878 nt long showing a typical velarivirus structure with 8 ORFs. Among them, the taxonomically important ORFs, ORF1a/1b encoding the polyprotein is 8 421 nt long, with a 0/+1 frameshift position at 6 923; ORF3 encoding the heat shock protein HSP70h is 1 656 nt long, and ORF6 encoding the coat protein duplicate is 1 989 nt long. The genome sequence showed its highest identity with LChV1 isolates Apr184R (96.2%), 19SP003B (92.2%) and Kyoto-2 (92.1%). A similar situation was also found for the ORF1, HSP70h, and CP nucleotide and amino acid sequences. A phylogenetic analysis identified Alm138 as a member of the G5 phylogenetic group, supporting its close relationships with the Czech Apr184R apricot isolate and the other members of this group. This report describes the first little cherry virus 1 isolate infecting almonds and confirms the presence of isolates of the G5 phylogroup within Europe.

Effects of eggshell powder supplementation on nutritional and sensory attributes of biscuitsOriginal Paper

Shahnila, Sana Arif, Imran Pasha, Hira Iftikhar, Fakiha Mehak, Razia Sultana

Czech J. Food Sci., 2022, 40(1):26-32 | DOI: 10.17221/309/2020-CJFS

Chicken eggshell, a poultry waste material, is a potential but poorly recognised source of calcium that can be used by humans to increase their dietary calcium intake by incorporating it into foods. This study was aimed at assessing the effects of eggshell powder supplementation at 5, 10, 15, and 20% levels on the chemical composition, sensory characteristics, and calorific value of the biscuits. Calcium absorption from supplemented biscuits was also determined. The inclusion of eggshell powder resulted in significant variations in the chemical composition of biscuits. It profoundly increased the mineral content, mainly calcium from 43.57 mg 100 g-1 to 1 054.7, 2 186.7, 2 941.6, and 3 843 mg 100 g-1 at 5, 10, 15 and 20% supplementation level, respectively. Substantial changes in the sensory quality of biscuits were also observed with corresponding rises in eggshell addition levels. Biscuits prepared with 5 and 10% supplementation levels were found acceptable in terms of sensory attributes. The in vivo study affirmed the absorption of calcium from eggshell powder and it was found highest (41.83%) at 5% supplementation level. Conclusively, supplementation of biscuits with eggshell powder might be an attractive source of dietary calcium intake without any significant adverse effects on biscuits quality up to 10% supplementation level.

Isolation and characterisation of Brucella melitensis by bacteriological and molecular methods from livestock in North CyprusOriginal Paper

M Demirpence, AM Saytekin, B Sareyyupoglu, OM Esendal

Vet Med - Czech, 2022, 67(10):497-509 | DOI: 10.17221/150/2021-VETMED

In this study, the isolation, biotyping and molecular characterisation of Brucella melitensis from cattle, sheep and goats in North Cyprus are reported on. A total of 319 raw milk samples obtained from seropositive dairy livestock (190 cattle, 74 sheep and 55 goats) and tissue samples including the liver, spleen and abomasal contents obtained from 32 aborted foetal samples (5 cattle, 18 sheep and 9 goats) were analysed for the presence and characterisation of the agent. B. melitensis was isolated and identified from 90 out of 319 (28.2%) milk and 19 out of 32 (59.4%) foetal samples by conventional bacteriological methods. Identification of all 109 isolates was confirmed by using real-time PCR with genus and species-specific primers. Following the preliminary identification, 27 selected isolates representing various counties and herds were further analysed by conventional methods. Twenty (74.1%) isolates were identified as B. melitensis biovar 1 and seven (25.9%) were identified as B. melitensis biovar 3. The Bruce-ladder multiplex PCR assay revealed that all the isolates were field strains. The results of the present study confirmed the presence of B. melitensis in livestock including the cattle population in North Cyprus. Even though the majority of the samples came from seropositive cattle, Brucella abortus was not isolated in the study. The results also revealed the potential public health risk of brucellosis in livestock emphasising the need of implementing strict control and eradication strategies against the disease in animal populations in order to protect human health.

Effects of washing procedures in reducing Listeria monocytogenes on raw leafy vegetablesOriginal Paper

Nguyen Thi Huong Giang, Mahmud Ab Rashid Nor-Khaizura, Nor Ainy Mahyudin, Thi Lam An Vu

Czech J. Food Sci., 2022, 40(6):422-426 | DOI: 10.17221/98/2022-CJFS

The efficacy of potable water and antimicrobial agents, including turmeric extract, black pepper extract, sodium chloride and sodium bicarbonate, in reducing Listeria monocytogenes on the leaf mustard (Brassica integrifolia) and iceberg lettuce (Lactuca sativa L.) was compared. The uncontaminated samples of two raw leafy vegetables were inoculated with L. monocytogenes at a concentration of 5 log and 7 log colony-forming units (CFU) g-1 for 1 h. At the high L. monocytogenes contamination level, the treatment with black pepper extract 0.6 mg mL-1 for 5 min was found to produce the most considerable decrease in L. monocytogenes counts, resulting in 1.44 log and 1.68 log reduction on leaf mustard and iceberg lettuce, respectively. Similarly, the black pepper extract also showed the highest L. monocytogenes reduction, approximately 0.79 log CFU g-1 on two leafy vegetables at the low contamination level. However, the odour of black pepper extract was left on the vegetables after being washed.

Afforestation of agricultural land affects soil structural stability and related preconditions to resist droughtOriginal Paper

Jiøí Holátko, Ondøej Holubík, Tereza Hammerschmiedt, Jan Vopravil, Antonín Kintl, Martin Brtnický

J. For. Sci., 2022, 68(12):496-508 | DOI: 10.17221/156/2022-JFS

Afforestation is important for the EU forest management strategy. Afforestation of abandoned and marginal arable land is a favourable non-agricultural land use option for climate change mitigation. It may prevent threats of drought or erosion e.g. by affecting the water balance in soil via increased structural stability. The structural stability control in afforested soil is related to i.a. organic matter content, nutrient content, soil reaction, planted tree species prosperity, and amelioration. A four-year field small-plot experiment on afforestation was carried out with Chernozem covered with deciduous (oak), coniferous (pine) or mixed planting, amended with 3 doses (no-application, 0.5 kg×m-2, and 1.5 kg×m-2) of alginite. In 2013 and 2016, soil reaction pHH2O, mean weight diameter (MWD), organic matter content (LOI) and total organic carbon (TOC) were determined and related to the soil structural stability to evaluate the soil precondition to sustain drought twice per vegetation period (spring and autumn). Afforestation significantly improved MWD compared to the field soil between 2013 and 2016 from 1.63 ± 0.04 mm to 1.85 ± 0.05 mm. Tree planting significantly neutralized the soil pHH2O, mixed planting appeared to improve LOI and TOC. Four-year afforestation led also to higher structural stability, less alkaline pH and deciduous tree-related increase in LOI, which may indicate better soil sustainability to drought.

Reducing the European Union's plant protein deficit: Options and impactsOriginal Paper

Hans Grinsted Jensen, Christian Elleby, Ignacio Pérez Domínguez

Agric. Econ. - Czech, 2021, 67(10):391-398 | DOI: 10.17221/94/2021-AGRICECON

The EU has a historical deficit of plant protein and is heavily reliant on imports to sustain domestic livestock production. Using an economic model of global agricultural markets, this article investigates three policy drivers that could have an influence on the increased production of protein-rich crops in the EU, namely coupled payments for protein-rich crops, investment in research and development leading to higher yields, and phasing out of imported high indirect land-use change risk biofuel feedstocks. Results indicate that a one per cent annual increase in yields over the medium-term has a much larger effect on EU domestic protein production than additional coupled payments of EUR 75 per ha. Moreover, phasing out palm-based biodiesel only has a small impact on protein self-sufficiency. A significant unknown is how costly it will be to increase the yields on protein crops grown in the EU.

State of the raw wood growing stocks and prediction of further development of cutting in the context of coniferous stands calamity in the Czech RepublicOriginal Paper

Dalibor ©afaøík, David Bøezina, Jakub Michal, Petra Hlaváèková

J. For. Sci., 2022, 68(10):423-435 | DOI: 10.17221/76/2022-JFS

The extremely dynamic development of calamities caused by the effects of global climate change followed by the spread of under-bark insect pests mainly in coniferous stands and the ongoing incidental felling have raised concerns in the woodworking industry about the developments in the source material with respect to ensuring production in a short-term view. Since the overall standing stock in spruce stands of all age classes in the Czech Republic amounts to 399.6 million m3 (2017-2026) and the theoretical outlook of the logging potential based on the percentage of logging accounts for 112.62 million m3 (2017-2026), the concerns might be deemed justified. The article presents an updated view of the current situation based on official statistics and offers an analytic prediction of the possible development, considering the possible consequences, even in the production of the forestry sector. The statistical data on the current situation have been compiled for the last two decades of development. The results show that with a continued high rate of bark beetle calamities, assuming a total annual cutting with a permanent limitation on the planned harvests of more than 30 million m3, the complete stands of spruce from age class 5 onwards could be harvested in approximately 14-16 years.

First sensory analysis of soybean drinks made from commercial grain grown in MexicoShort Communication

Mónica López-Fernández, Octelina Castillo-Ruíz, Homar Rene Gill-Langarica

Czech J. Food Sci., 2022, 40(5):400-406 | DOI: 10.17221/153/2021-CJFS

In Mexico there is an important production of soybeans; however, no evidence of the analysis of organoleptic attributes in Mexican soybean products was found, so the objective of this work was to sensorially characterise soymilk made from commercial beans grown in northern Mexico based on seven organoleptic attributes. A panel of trained judges evaluated 11 soymilks made from commercial soybeans grown in Mexico, two soymilks made from commercial soybeans reported to be free of lipoxygenases, and one control soymilk. Evaluated flavour attributes were beany, milky, greasy, toasted, bitter, metallic and rancid. The results showed that the soymilks from the JP 30790 and JP 28955 genotypes generated low beany notes. The Mexican variety Guayparime S-10 obtained the lowest beany flavour note. Among Mexican soybeans, the soymilks of Huasteca 700 and Vernal varieties got the highest values of the milky flavour and the JP 30790 and JP 28955 materials obtained intermediate values in this attribute; in the rancid attribute, the zero value was given to all the soymilks. The scarce beany notes and the high milky attribute, as well as the zero value in the rancid attribute favour an acceptable flavour in the soymilks.

Comparison of acid and alkaline pre-treatment of lignocellulosic materials for biogas productionOriginal Paper

Barbora Jankovièová, Miroslav Hutòan, Marianna Nagy Czölderová, Kristína Hencelová, Zuzana Imreová

Plant Soil Environ., 2022, 68(4):195-204 | DOI: 10.17221/421/2021-PSE

This work deals with the study of a pre-treatment method promoting degradability of lignocellulosic biomass and hence biogas yield therefrom, as this material is challenging to decompose due to its structure. The investigated pre-treatment methods are hydrolysis of the material in NaOH (0.5% and 5%) and H2SO4 (0.5% and 5%) at temperatures of 90-100 °C for 2 h. This work aimed to compare the effects of these pre-treatment methods on the lignocellulosic composition of maize waste (maize stalks, leaves and cobs), rapeseed straw and wheat straw and the biogas yields from its subsequent anaerobic digestion. Pre-treatment by 0.5% NaOH increased the biogas production the most (by 159% for rapeseed straw, 240% for wheat straw and 59% for maize waste) also the degrees of solubilisation was higher, at the same time the proportions of total organic carbon (TOC) in the solid component appear to be sufficiently high, it could be assumed that there has been sufficient disturbance of the material structure. Alkaline methods have proven to be more suitable compared to acid pre-treatment methods, also the use of alkali with a lower concentration has shown to be more efficient, which is more advantageous for use in practice from an environmental and economic point of view.

Status, challenges and opportunities for apple production in Eastern AlgeriaOriginal Paper

Sanna Abdessemed, Ahmed Fellak, Ala Abdessemed, Awais Khan

Hort. Sci. (Prague), 2022, 49(3):147-153 | DOI: 10.17221/103/2021-HORTSCI

Apples (Malus domestica) are one of the most important temperate fruit crops in the world. Apple production provides, directly or indirectly, employment and improvement in the living standards to millions of people, playing a key role in rural economies. We have conducted an interview-based survey to assess the current status and challenges faced by apple production in the Batna and Khenchela provinces, the two most important apple producing regions of Eastern Algeria. The survey was conducted between March and November 2019. A total of 50 apple growers answered 40 questions, including 13 direct and 20 qualitative questions. A scale ranging from 1 (very low) to 5 (very high) was used as a quantitative measurement for 12 questions. The reliability index of the questionnaire was 0.827. The results identified the main challenges faced by apple growers in Eastern Algeria as: limited access to certified and clean planting material, limited knowledge of disease and pest identification, inadequate access to appropriate disease management, lack of storage facilities and transportation of harvested fruit, and insufficient knowledge of marketing and access to markets. The study also identified a need for the development of cost-effective plant multiplication, horticultural and disease management practices and educational programmes suitable for local conditions to enhance the productivity and economic benefits of apple orchards.

Effects of different substrates on the runner production of the June-bearing strawberry cv. 'Senga Sengana'Original Paper

Vahid Avdiu, Bedri Dragusha, Egzona Hajra, Gezim Hodolli

Hort. Sci. (Prague), 2022, 49(4):197-204 | DOI: 10.17221/27/2022-HORTSCI

Producing high-quality daughter plants of the June-bearing strawberry is very important for fruit production around the year. This study was conducted to investigate the impacts of different substrates on the runner production of the June-bearing strawberry cultivar 'Senga Sengana'. In April 2019, strawberry mother plants were planted while the daughter plants were examined November 2019. The nursery trial field was separated into four substrate treatments, each included ten strawberry mother plants, approximately homogeneous. The place where the mother plants were planted was with the same substrate (PTS substrate "Pindstrup") and separated from the field for the rooting of the daughter plants. In the field for the rooting of the daughter plants, four types of substrates were used: The first substrate treatment (S1) (soil 50% - decomposed manure 50%); the second substrate treatment (S2) (PTS substrate "Pindstrup" with modules (pots) with a volume of 200 mL/daughter plant); the third substrate treatment S3 (river sand 50% - soil 50%); lastly, substrate treatment four S4 (sawdust 100%). During the research, several parameters were determined for the mother plants: the crown diameter, number of flowers, number of runners per plant, number of daughter plants per runner and number of daughter plants per plant. The examination of some of the qualitative parameters was also performed on the daughter plants: the crown diameter, root length and number of roots. Based on these parameters, the daughter plants were categorised according to the standards: A++, A+, A, A-, OS (out of the standard). From the examined results, it was concluded that S3 formed the largest number of daughter plants at 123 (A++ 19.1, A+ 45.1, A 28.7, A- 17.8, OS 12.3 plants); S2 formed 69.4 daughter plants, but with the highest standards (A++ 34.7, A+ 33.6, A- 1.1 plants); S4 formed 74.8 daughter plants (A++ 26.2, A+ 30.4, A 14 OS 4.2 plants), while S1 formed 62 daughter plants and showed the worst results for both the number of plants and the standard (A+ 7, A 3.8, A- 0.8, OS 50.4 plants).

Comparison of the physical properties of soils belonging to different reference soil groupsOriginal Paper

Jan Vopravil, Pavel Formánek, Tomá¹ Khel

Soil & Water Res., 2021, 16(1):29-38 | DOI: 10.17221/31/2020-SWR

Soil properties can be influenced by long-term agricultural management practices as described in pedological literature. In this study, selected physical properties (particle density and bulk density, total porosity, maximum capillary water capacity, minimum air capacity, field capacity, permanent wilting point and available water capacity) of topsoils from different reference soil groups (Cambisols, Luvisols, Fluvisols, Chernozems and Phaeozems, Leptosols, Stagnosols and Gleysols) were sampled and analysed in the years 2016-2017. The topsoil samples were taken from points of so-called S (specific) soil pits to be sampled from the General Soil Survey of Agricultural Soils (GSSAS) which was accomplished in the years 1961-1970. In addition, some of the properties were also compared with those measured during the GSSAS. Recognising the properties, only the particle density, the maximum capillary water capacity, the permanent wilting point and the available water capacity of the topsoil of the individual soil groups were statistically significantly (P < 0.05) different. A comparison of the physical properties with those analysed after more than 40 years was performed, the bulk density increased and the total porosity decreased in the topsoil of the major part of the studied soil groups.

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