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Pot experiments were carried out to study the effects of a novel stabilizer of 2,4,6-trimercaptotriazine, trisodium salt, nonahydrate (TMT) on the bioavailability of heavy metals (Cu, Zn, As, Cd and Pb) in soils and heavy metals accumulation in tobacco. The results showed the optimal TMT dosage 200 mL/kg could effectively reduce the bioavailability of heavy metals in soils, and the bioavailability of Cu, Zn, As, Cd and Pb in soils was reduced simultaneously by up to 73.1, 63.2, 48.0, 68.9 and 57.2%, respectively. Application of TMT could significantly decrease Cu, Zn, As, Cd and Pb contents of all parts of tobacco. Furthermore, the contents of available Cu, Zn, As, Cd and Pb in soils were significantly positively correlated with Cu, Zn, As, Cd and Pb contents in upper, middle and lower leaves and stems of tobacco. There were no significant differences among plant height, number of leaves, stem girth, length and width of maximum leaves of tobacco under TMT treatments and that under control (P < 0.05), which showed that the TMT did not promote and inhibit the growth of tobacco.

The objective of this study was to compare the effects of calf starters of different starch and fibre content on the growth, blood and rumen parameters of Holstein female calves during the pre-weaning period. A total of 60 calves were divided into two groups. Group A/B was fed according to a two-phase calf nutrition system: the calves received calf starter "A" (28.5% starch content, 16% neutral detergent fibre (NDF), 9.2% acid detergent fibre (ADF)) in days 7-45 and then calf starter "B" (14.2% starch content, 32.4% NDF, 14.3% ADF) in days 46-70. Calves of group B received calf starter "B" throughout the experimental period. The calves were weaned between 52 and 61 days of life. The data on body weight, weight gain, starter feed intake, blood metabolites and rumen fermentation parameters were collected individually. In the first phase of experiment, the starter feed consumption of A/B group was higher than that of B group (P < 0.05), but it did not manifest in differences in body weight at weaning time. At some of the sampling times, the different composition of starter diets significantly (P < 0.05) influenced the plasma urea, albumin, glucose and triglyceride concentrations. The consumption of diet with different starch and NDF content had no significant effect either on the composition of the rumen microbiota or on the ruminal concentration of volatile fatty acids (VFA) and ammonia. In some cases significant correlations (-0.83 and -0.93; 0.82 and 0.90) were found between the composition of rumen microbiota and ruminal VFA concentration. Feeding a diet of higher starch content (28.5 vs 14.2%) in the first 45 days of life did not affect rumen fermentation, rumen microbiota and the most important blood metabolites, apart from a few exceptions. The production parameters of calves, including total feed intake and body weight gain, were similar in the experiment.

The pathogenicity of bacterial strains isolated from pathological processes and lesions of horses, strategies for their treatment and the choice of appropriate antimicrobials are frequently a challenging problem for private veterinarians who seek help in our laboratory. Therefore, the aim of this study was to map genera and species of Gram-negative aerobic and microaerophilic microorganisms isolated from pathological processes in horses and to identify the most effective antimicrobial agents for therapy based on antibiotic susceptibility. Between 2009 and 2014 a total of 449 clinical samples (n = 449) were examined; 229 (51%) of them were obtained from the respiratory tract, 121 (27%) from the skin, 40 (8.9%) from the digestive tract, 40 (8.9%) from the eyes, eight (1.8%) from the urinary system, six (1.3%) from the musculoskeletal system, four (0.9%) from the lymphatic system and one (0.2%) from milk. The examination was performed using conventional microbiological culture methods. The identification of isolates was confirmed using MALDI-TOF molecular phenotyping (Bruker Daltonics GmbH, Bremen, Germany). From the 276 Gram-negative isolates (prevalence of 61.5%), the most frequently detected strains were Enterobacter spp., Escherichia spp., Acinetobacter spp., Pseudomonas spp. and Actinobacillus spp. with prevalence rates of 7.6%, 6.7%, 6.7%, 6.0% and 5.8%. In addition, another 20 genera of microorganisms were detected. Susceptibility to antimicrobial agents was determined using the disc diffusion method. The most effective agents were gentamicin (94.1%), enrofloxacin (91.7%), colistin (87.0%), florfenicol (86.2%), neomycin (85.5%), streptomycin (82.4%) and tetracycline (78.5%). A good knowledge of the spectrum of bacterial species participating in pathological processes and lesions in horses and their antimicrobial susceptibility may be of great importance not only in treatment but also in deciding which prophylactic antibiotics to administer after surgical interventions.

The aim of this study was to evaluate the effectiveness of deslorelin acetate in the regulation of reproductive activity in captive leopard geckos (Eublepharis macularius). Fourteen healthy adult females were separated into two groups. Under general anaesthesia, deslorelin acetate implants (4.7 mg) or placebo implants were administered into the coelom of ten female geckos and four female geckos, respectively. One healthy adult male Leopard gecko was added to each group of females (five females with GnRH implants and two females with placebo implants). The geckos were regularly monitored over two breeding seasons (visual examination, weight control). Nesting sites were checked daily. There were no postoperative complications or any other health problems during the study. Implant administration did not result in long-term suppression of reproductive function. No significant differences were found in the number of clutches between the female groups (deslorelin implants versus placebo implants) or in the number of clutches between the two breeding seasons. Deslorelin acetate implants did not interfere with ovarian activity in captive female leopard geckos. The use of GnRH agonist implants is not an appropriate method for control of reproductive function in female leopard geckos.

European fruit lecanium, Parthenolecanium corni (Bouché, 1844) is among the leading worldwide pests of fruits such as plums, currants, jostaberry, grapevine and many others. This study is focused on testing the effectiveness of natural insecticide treatments compared with conventional insecticides on overwintering nymphs of the European fruit lecanium. In February of 2017, two experiments were conducted under laboratory conditions. The tested products were Boundary SW^® (plant extract), Konflic^® (plant extract), Ekol^® (canola oil) and a tank-mix of Ekol^® + Reldan 22^® (canola oil and chlorpyrifos-methyl) as a chemical standard. The most effective product tested was the chemical standard tank-mix of Ekol^® and Reldan 22^®, which was 100% effective in all experiments. High efficiency was also achieved with Ekol^® (canola oil) in high concentrations. We can summarize, that using canola oil in high concentration has almost the same effect as the chemical standard. Using canola oil against European fruit lecanium meets the principles of Integrated Pest Management (IPM) and also the need to design cropping systems that are less dependent on synthetic pesticides.

In the paper, the price volatility was examined. The authors used 650 weekly observations from 2003 to 2015. Such a long period of analysis helped to reveal periods with high volatility. The objective of the paper was to recognize price volatility of agricultural commodities in Poland. The authors chose beef, pork and wheat markets to show the differentiation of price volatility. It revealed periods of large and small volatility. The global market situation impacted Polish agricultural markets with the opening markets and a greater access to the new markets. The periods having the strongest impact on Polish agricultural markets were the integration with the EU, the global crisis in 2008, and problems in the EU zone. The prices of analysed agricultural commodities differed in various EU countries. The prices of wheat increased most in France, Hungary and Lithuania. The prices of store cattle increased most in the years 2004-2015 in Estonia, Sweden and Luxemburg. The prices of pigs increased most in Malta, Sweden and Cyprus.

The paper deals with Czech households' subjective views on food waste. It examines households' attitudes to food waste, including the causes of food waste. The primary data were obtained via a questionnaire survey with 1 582 respondents. The analyses allowed the identification of three categories of Czech households with different attitudes to food waste and its causes. These findings provide a basis for a proper selection of corrective measures aimed at amending or even completely transforming the current behaviour.

Crop growth is a key factor that effects nitrous oxide (N₂O) emission in farmland soil. Clarification and quantification of the impact of maize growth on N₂O emission are important to guide maize planting and patterns, which is also useful for building model to simulate N₂O emission in an agroecosystem. In this study, we carried out a three-year (2013-2015) field experiment to evaluate the contribution of maize growth on N₂O emission using a split-plot design. The factors included planting versus not planting maize, and four rates of nitrogen (N) application (0, 150, 300, 450 kg N/ha). Our results showed the impacts of maize growth on N₂O emission decreased linearly with the growth of maize from the 43^rd day after sowing (y = -1.07x + 26.85, R² = 0.95). Nitrogen fertilizer application can reduce the impacts of maize growth on N₂O emission. The impact of maize growth on soil NH₄⁺-N and NO₃^--N are similar to N₂O emission, and they have a strong correlation. We concluded that maize growth reduces soil N₂O emission but N application can exert an antagonistic effect, and the impact of maize growth on soil NH₄⁺-N and NO₃^--N largely determines the impacts of maize growth on N₂O emission.

The aim of the research was the determination of the tolerance of different pear grafting combinations to various levels o lime concentration in calcareous soils. The experiment was carried out under pot conditions for a two-year period (2015-2016). Two factors including combination of rootstocks/scions (3 × 3) and levels of lime concentration were employed to evaluate leaf responses including total iron (Fe), available Fe, chlorophyll (Chl) (a, b, total), and chlorophyll fluorescence attributes (F0, Fm, Fv/Fm) as well as annual growth and internode length of current shoots. Results showed that soil lime significantly reduced tree growth but conversely intensified iron chlorosis. Also, contrary to our expectations, the Pyrodwarf/Pyrus communis L. (Dargazi) combination displayed more tolerance to high lime concentrations, whereas the OH × F/Williams Duchesse combination did not exhibit suitable tolerance. As a pear rootstock native to Iran, Dargazi seedling rootstock in combination with different scions was found to have relatively better growth under low lime concentrations, but its response under high lime was not as favourable as expected. Cultivar Dargazi combined with different rootstocks showed a better response to high lime stress compared to other scions, while cultivar Williams Duchesse exhibited lower tolerance to high lime concentration when combined with different pear rootstocks.

The study analyses the correlations among different economies of selected EU-12 member states based on comparison of agricultural economics variances, namely the output value of the agricultural industry, productivity of input, agricultural gross value added, subsidies on production, agricultural labour input and agricultural income per annual working unit in the period of 2010-2016, based on the Special Program for Social Sciences, as statistical methods. The EU-12 achieved a higher increase in productivity of input, output value of agricultural industry, agricultural gross valued added, as well as agricultural income per agricultural annual working unit compared to the average results of EU-28 for 2010-2016. The output value of agricultural industry and agricultural gross value added per intermediate consumption decreased by 1.35% and by 3.3%, but the factor income - net value added at factor cost - per annual working unit increased by 21%, because of the subsidies on production increased by 3.4% for 2010-2016. In EU-28, the factor income per annual working unit increased, but most of this income was for developing agricultural production technology.

Soil samples from 100 cultivated and natural sites were assessed for the presence of entomopathogenic nematodes. Heterorhabditid nematodes were recovered from three soil samples during spring months, with the overall positive sample rate of 3%. The isolates of entomopathogenic nematodes were identified as three different strains conspecific with Heterorhabditis bacteriophora (Heterorhabditidae). They were found from natural sites and vineyard, while no recovery occurred from intensively cultivated agricultural fields. The morphometrical characteristics of infective juveniles and males showed differences between all Croatian strains and from the original description. Heterorhabditis bacteriophora ISO9 was bioassayed on Lasioptera rubi (Cecidomyiidae) (the raspberry gall midge) larvae at different nematode concentrations under laboratory conditions. The significantly highest mortality was observed in treatments with 50 and 200 infective juveniles per insect larvae within 8 days after inoculation. This is the first report of entomopathogenic nematodes of the family Heterorhabditidae from Croatia, and susceptibility of L. rubi larvae to entomopathogenic nematodes. The Croatian strain H. bacteriophora ISO9 was proved to possess strong insecticidal properties against L. rubi larvae.

A field experiment was carried out in the years 2012-2018 in Poland in a split-plot design. The aim of the study was to determine the long-term effect of legumes as forecrops on the productivity of rotation with nitrogen fertilization. The rotation included: legumes + spring barley (SB), winter rape (WR), winter wheat (WW) and winter wheat. The study was conducted as a two-factorial field experiment with four replications. The present study showed that legumes as forecrops increased the yield of all after-harvest crops in rotation. Yielding of these crops also depended on nitrogen fertilization and position in the rotation. After comparison of the influence of nitrogen fertilization on yield of cereals, it was observed that the effect of this factor was greater for WW cultivated in the fourth year of rotation than for WW cultivated in the third year of rotation. In relation with control, each dose of nitrogen fertilization caused a significant increase of WR and cereals yield, but the dose of 180 kg N/ha did not increase yield significantly in comparison to the dose of 120 kg N/ha. There was also negative agronomic N-efficiency observed between doses of 120-180 kg N/ha, which means that it is not necessary to use 180 kg N/ha, especially if there are legumes in crop rotation.

The objective of the present study was to estimate the effect of breed on milk fatty acid (FA) composition of dairy (Brown Swiss, Holstein-Friesian, and Jersey) and dual-purpose cows (Simmental and Alpine Grey) in multi-breed herds. Information on individual milk samples was collected during routine cow milk testing between 2011 and 2014, and consisted of 285 606 observations from 17 445 cows in 617 herds. Fixed effects included in the mixed model were breed, parity, stage of lactation and the interaction between parity and stage of lactation, and random effects were cow, herd-test-date and residual. Contrast estimates for the studied traits were used to compare specific sets of breeds. Holstein-Friesian produced more milk than the other cattle breeds, with the greatest trans FA and C18:1 and the lowest C18:0 content. Comparison between the specialised dairy vs the dual-purpose breeds highlighted significant differences for all traits except for polyunsaturated FA and trans FA content. Specialised dairy breeds had greater milk saturated FA, short-chain FA, medium-chain FA, C14:0 and C16:0 content, and dual-purpose breeds produced milk with greater content of monounsaturated FA, long-chain FA, C18:0 and C18:1. Results demonstrated that, although specialised dairy produced more milk than dual-purpose breeds, milk FA profile of the latter was more favourable from a human nutrition point of view.

To assess the response of promising safflower genotypes to late-season drought stress in delayed planting conditions, an experiment was conducted in two years (2016-2017 and 2017-2018) in Iran. The irrigation regime was specified in two levels, including normal irrigation and irrigation cut off in the seed filling stage in main plots, and five safflower genotypes, including Soffe, Goldasht, Golmehr, Padideh, and Parnian were categorized in subplots. Applied drought stress significantly reduced the seed yield and yield components of all genotypes, which accompanied with a substantial decrease in oil content and oil yield of all genotypes. However, the highest seed and oil yield in drought stress conditions obtained in the Parnian genotype by value 2338 and 561 kg/ha, respectively. Moreover, a significant drought-induced increase in palmitic, stearic, and oleic acids, as well as a decrease in linoleic acid content, was observed in all genotypes. Parnian genotype with high unsaturated fatty acids content (90.9%) and the minimum amount of saturated fatty acids (8.7%) might be a promising genotype to starting a formal crop improvement program to achieve more drought-tolerant safflower genotype.

A 6-month-old crossbred of a Holstein and Japanese Black heifer calf weighing 95 kg presented with a history of intermittent abdominal distension and failure to thrive. The physical examination identified a pinging sound over the dorsal left flank. The abdominal radiography showed a huge gas-filled mass. The intravenous urography revealed no communication between the mass and the urinary bladder. Although the visual examination and palpation of the umbilicus did not reveal visible abnormalities, an umbilical disease was suspected because the animal exhibited poor growth, depression, and a hunched back posture. When the eschar adhering to the centre of the umbilicus was removed, the presence of a fistulous tract was revealed. The umbilical ultrasound examination revealed an intra-abdominal abscess and the fistulography demonstrated that the abscess communicated with the umbilicus. The abscess, compressing into the rumen, was observed by computed tomography. From these images, it was diagnosed as an umbilical cord remnant abscess and a definitive diagnosis of a urachal abscess was obtained by open abdominal surgery and the subsequent removal of the mass. The calf was discharged from the university hospital on day 14 after the operation. This case shows that a urachal abscess should be considered when a pinging sound is present, even if the animal exhibits no swelling or pain of the umbilicus.

The effect of taxifolin (TXF) with starter cultures (SC), such as Leuconostoc carnosum, or a mixture of strains Pediococcus pentosaceus and Staphylococcus xylosus, on the TXF stability was evaluated. UPLC analysis demonstrated that after 181 days of storage total TXF content was the highest in samples with TXF and L. carnosum (60%), compared to the 1st day of storage. The sausages with TXF and the mixture of P. pentosaceus and S. xylosus (56%) followed next. The samples treated only with TXF retained 40% of TXF, compared to the 1st day of storage. TXF had no significant effect on the growth of lactic acid bacteria. The accumulation of biogenic amines (BA), including histamine and putrescine, was more effectively reduced in sausages inoculated with the TXF plus P. pentosaceus and S. xylosus mixture. Using this mixture, the rate of lipolysis and processes of lipid oxidation were effectively slowed down. Fatty acid (FA) composition was stable in all cases.

Optimal soil nitrogen management is vital to crop production and environment protection. Little knowledge is available on crop yield, nitrogen uptake, use efficiency and balance in paddy rice-upland wheat cropping system of China. A thirty-five-year long-term field experiment was designed with nine treatments, including an unfertilized treatment (control), nitrogen (N), phosphorus (P), potassium (K) fertilizer, manure (M), and manure combined with mineral fertilizer treatments. Crop yield, N uptake, use efficiency, and N surplus or deficit amount were determined. The results indicated that rice, wheat yield and N uptake amount in the manure combined with mineral fertilizer treatments were higher than that in the manure alone or mineral fertilizer alone treatments. N use efficiency was the highest in the treatment with manure alone. Soil N input indicated a surplus in the mineral fertilizer in combination with manure treatment, but soil N input indicated a deficit in the control, NPK and M treatments. Considering crop yields, N use efficiency and N balance, recommended N application amount is almost 220 kg N/ha/year in the paddy rice-upland wheat cropping system. Taking into account labour and fertilizer sources, half mineral N and half organic N applications were recommended.

The efficacy of using maize (Zea mays L.) as a suitable substitute for other crops with high arsenic (As) accumulation in As-contaminated farmlands remains debated. Here, the As uptake capacity and the stability of accumulated As of different maize cultivars were studied using pot and field experiments, outdoor investigations and literature data analysis. When the total and available soil As levels were 238.8 and 8.1 mg/kg, respectively, grain As ranged from 0.03 to 0.07 mg/kg, significantly lower than the acceptable As limit (0.5 mg/kg) for maize in China. The results of field investigations and literature data analysis also supported this observation. Maize is a crop with low grain As, thus, making it suitable for substitution planting in As-contaminated farmlands. Further, grain As concentration varied among different maize cultivars. The planting of normal and waxy maize is prioritized over the sweet maize as the first one has lower available bio-concentration factor (aBCF) of 0.007 for grain and higher accumulated As stability among its cultivars (CV < 10%) than those for sweet maize (aBCF = 0.01 and CV = 35.5%). Arsenic compartmentalization in the roots and low As upward migration into the grain were responsible for the low grain As of maize.

This study aimed to evaluate the impact of selection on the genome structure of beef cattle through identification of selection signatures reflecting the breeding standard of each breed and to discover potential functional genetic variants to improve performance traits. Genotyping data of six beef breeds (Aberdeen Angus, Hereford, Limousin, Charolais, Piedmontese and Romagnola) were used to perform genome-wide scans for selection signatures. The approaches applied were based on an assumption that selection leads to linkage disequilibrium or to a decrease of genetic variability in genomic regions containing genotypes connected with favourable phenotypes. Thus, the selection signatures were analysed based on Wright's F_ST index, distribution of runs of homozygosity segments in the beef genome and determination of linkage disequilibrium variability between breeds. The number and length of detected selection signals were different depending on the breeds and methodological approaches. As expected due to the breeding goals of analysed breeds, common signals were located on autosomes 2, 6, 7, 13 and 20 close to the genes associated with coat colour (KIT, KDR), muscle development (GDF9, GHRH, GHR), double muscling (MSTN), meat tenderness (CAST) and intramuscular fat content (SCD). But, across the genomes of analysed breeds, unique selection signals were found as well. The subsequent analysis of those single nucleotide polymorphism markers can be beneficial for the genetic progress of studied breeds in future.

Proper vascularisation is necessary for the correct functioning of all organs. The kidneys of various mammalian species have been examined in order to understand the functioning of this organ. This article presents the first classification of the renal artery division in the kidneys of adult cattle. We collected and analysed specimens of arteries from bovine kidneys with the aim of improving our understanding of their morphology and functioning. The study was conducted on 50 kidneys, 25 right ones and 25 left ones, taken from cattle of both sexes. The examined kidneys were dissected and corrosion casts were made. Division of the renal artery into between two and four primary segmental arteries takes place just before entering the renal hilum. Cranial primary segmental arteries number from one to two (most frequently one), whereas the hilar and caudal ones always occur singly. The mentioned vessels are then divided into between one and four secondary segmental arteries running within the renal sinus. The hilar region (mid-zone) of the kidney exhibits the most variation in terms of vascularisation. The vascularisation of the caudal pole exhibits the lowest degree of variation. Taking into consideration the range of vascularisation of the organ by the particular divisions of the renal artery, three renal branching pattern types were distinguished: type I (84.1% of cases; this type consists of Ia, Ib, Ic and Ic subtypes) - two branches - the renal artery is divided into cranial and caudal primary segmental arteries; type II (11.36% of cases; with IIa and IIb subtypes) - three branches - the renal artery is divided into the cranial, hilar and caudal primary segmental arteries; type III (4.54% of cases) - four branches - the renal artery is divided into two cranial, one hilar and one caudal primary segmental artery. The division of the renal artery takes place along the long axis of the organ. Bovine kidneys are characterised by asymmetry, which may influence the length and diameter of the main arteries. The caudal primary segmental artery has the biggest mean length and mean diameter. The division of the renal artery occurs just before it enters the renal hilum or in the renal sinus.

The aim of this study was to assess the effects of four different drugs used for anaesthesia premedication on intraocular pressure and pupil size in dogs. A prospective, randomised, double-blind clinical study was carried out. The subjects were forty client-owned healthy dogs (20 males and 20 females), aged 8.0 ± 2.9 years, with body weights of 11.8 ± 8.5 kg (mean ± SD) and without ocular abnormalities that were scheduled for periodontal treatment. Animals were randomly allocated into four groups and received intravenously either medetomidine 0.01 mg/kg, acepromazine 0.02 mg/kg, fentanyl 0.01 mg/kg or butorphanol 0.2 mg/kg. Intraocular pressure, pupil size, heart rate, respiratory frequency and systolic and diastolic arterial pressures were measured prior to (baseline) and at five and 10 minutes after premedication (T5, T10). Data were analysed by Anderson-Darling, Bartlett's, ANOVA and Dunnett's tests (P < 0.05). Significant increases of intraocular pressure were observed at T5 and T10 in the fentanyl group. Significant decreases of pupil size at T5 and T10 were detected in the fentanyl, butorphanol and medetomidine groups. In the fentanyl group, heart rate dropped significantly at T10, while respiratory frequency was elevated at T5 and T10. In the medetomidine group, heart rate and respiratory frequency were decreased at T5 and T10. In the butorphanol group, systolic arterial pressure was decreased at T5 and diastolic arterial pressure was decreased at T5 and T10. In the acepromazine group, systolic arterial pressure was decreased at T10. Within ten minutes after intravenous administration in healthy dogs, fentanyl significantly increased intraocular pressure and fentanyl, butorphanol and medetomidine decreased pupil size.

A number of variables influences potatoes growing, including natural conditions, used growing technologies and market conditions. The most important parameters for the production of potatoes crops are yield, farmer's price, subsidies and costs. All these parameters can change over time. This means that managers of farms must constantly assess the key parameters affecting the economic outturn and analyse the degree of risk of their achievement. This article analyses the economic risks of potatoes cultivation based on statistical data obtained over the last 10 years. The Monte Carlo stochastic simulation method was used to analyse the risk of gross profits. The results of the calculations confirmed the considerable variability and risk of growing potatoes in the climate conditions of the Czech Republic in general, and especially regarding the first early potatoes and potatoes for starch production.

Cortisol, which is produced in the adrenal glands, is an endogenous glucocorticoid hormone that delivers its hormonal message to cells by acting on glucocorticoid receptors. It is one of the main stress hormones responsible for stress responses in animals and humans, and its overproduction is characteristic of certain diseases. While acute stress disorder can be evaluated by means of measuring the cortisol concentration in blood and urine, chronic stress disorder can be detected by monitoring the cortisol concentration in fur or hair. Hair collection is simple, inexpensive and non-invasive, and can be performed easily and rapidly; thus, it appears to be a suitable method for determining the level of stress in dogs from shelters, abused dogs or dogs involved in different types of animal interactions. Since it is a relatively new method, monitoring cortisol in hair or fur requires further research in order to definitively prove its efficacy, and possibly to determine reference range values for different breeds of dogs.

Two Hanwoo steers were emergency-slaughtered due to symptoms of urethral obstruction. The steers were of the same age and were raised using the same feed management. The remaining 22 cattle in the finishing period showed no symptoms of urinary calculi until 30 months of age and were examined for the presence of bladder calculi when slaughtered. Bladder calculi were observed in 11 of 22 steers at slaughter (50%) and cystitis occurred in 13 of 22 steers (59.1%). There was a significant relationship between the presence of bladder calculi and the incidence of cystitis (P < 0.05).

To describe transscleral ultrasonography as a novel alternative approach for visualising the optic nerve sheath and measuring its diameter and to analyse the linear regressions of the optic nerve sheath diameter value with the weights and morphometric measurements of the globe in dogs. Forty healthy dogs admitted for routine sterilisation were examined. Under general anaesthesia, a B-mode ultrasonography with a linear probe (9-18 MHz) was applied transscleral in the dorso-temporal quadrant. The optic nerve sheath diameter was measured 3 mm behind the caudal aspect of the globe. The morphometric measurements, including the axial globe, lens thickness and vitreous chamber, were estimated by two observers using the direct corneal approach. Univariate and multivariate multiple linear regression analyses were performed to explore the associations of the independent predictors with dependent variables. The optic nerve sheath diameter intraclass correlation coefficient (ICC) analyses revealed interobserver 0.91 (ICC = 0.83-0.95) and intraobserver 0.93 (ICC = 0.87-0.96) reliability. The multiple regression analysis revealed that the optic nerve sheath diameter was associated with the weight (R² = 0.60, P < 0.0001) but not with the axial globe (P = 0.48), the lens thickness (P = 0.73) or the vitreous chamber (P = 0.99). The findings of this study suggest that transscleral ultrasonography may be a valid alternative approach for the optic nerve visualisation and optic nerve sheath diameter measurements with excellent intra- and interobserver repeatability. The optic nerve sheath diameter was associated with the body weight, but not with the morphometric measurements of the globe.

Sweet cherries are self-incompatible, which is determined by a gametophytic self-incompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic S-locus. Knowledge about the S-allele constitution of the cultivars is essential for fruit growers and breeders. Recently, molecular PCR-based methods have been developed to distinguish all S-alleles in sweet cherries. In our work, we analysed S-locus genotypes by 13 universal and allele-specific PCR primer combinations within 117 registered, old and local sweet cherry cultivars from the Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy, the Czech Republic. We confirmed the previous S-genotyping for 66 accessions except for Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Badeborner, Bing, Alfa, Gamma, Huldra, Rivan, Valerij Tschkalov, Viola and Winkler's Frühe. It could be due to either mislabelling or mistakes in the previous analyses. Newly, S-genotyping was determined for 51 accessions in which we found 4 new S-loci combinations. We detected the S-locus combinations in 19 incompatibility groups. The most frequent incompatibility groups were III (S₃S₄), II (S₁S₃), IV (S₂S₃), and VI (S₃S₆) with 22, 20, 12 and 12 genotypes, respectively.

Common wheat, a fundamental commodity on international markets, is increasingly differentiated into commercial types on domestic markets to meet the demand of processing companies. Improver wheat, biscuit wheat, ordinary and superior bread-making wheat are commercial varieties with specific technological characteristics. Wheat farming systems are constantly evolving, and as a result, related environmental issues emerge. We applied an LCA (Life Cycle Assessment) analysis, where the functional unit was 1 tonne of grain for each typology and system boundaries were from cradle to farm gate. Primary data were used in the study, and special attention was paid to fertiliser use. From an LCA perspective, our findings show that nitrogen (N) plays an essential role in plant production although producing different waterborne and airborne emissions and nitrate leaching, for the 4 commercial typologies studied. Furthermore, the impact can be differentiated based on the technological features of the commercial types. Our results led us to observe that the four wheat types show contrasting economic and environmental performances.

Limnocharis flava (L.) Buchenau is among the most problematic rice weeds in Malaysia and is also reported to have developed multiple resistance to AHAS inhibitor bensulfuron-methyl and synthetic auxin 2,4-D. In this study, resistance across different AHAS inhibitors was characterised in a L. flava population infesting rice fields in Pulau Pinang, Malaysia. Dose-response experiments were conducted to determine the level of resistance to sulfonylureas, imidazolinone, triazolopyrimidine, and pyrimidinyl-thiobenzoate. Cross-resistance across different AHAS inhibitors was observed in the resistant L. flava population, exhibiting a high level of resistance to bensulfuron-methyl, while exhibiting a moderate level of resistance to metsulfuron-methyl and a low level of resistance to pyrazosulfuron-ethyl and pyribenzoxim. However, all resistant L. flava individuals were still sensitive to imazethapyr, penoxsulam, and bispyribac-sodium. Based on the results, it is likely that resistance to AHAS inhibitors in L. flava is conferred by target-site resistance mechanisms.

Symptoms of non-specific bark necroses in beech trees of all ages have lately been described. We investigated the occurrence of bark necrotic disease in beech thicket and health status of the trees. Our objective was to examine how bark necrotic wounds persist and the regenerative potential of young beech thicket. The research experiment was carried out at two isolated localities in the Western Carpathians in the period 2015-2017. A terminal shoot (stem) and a lateral shoot (branch) of each of the 30 sampled beech trees were examined. Young beech stands were affected by the necrotic disease in 87-94% cases (stems 69-83%, branches 33-56%). Shoot diameter and age had a significantly negative linear correlation (P < 0.05) with the development of all analysed disease characteristics on the beech bark. The encouraging finding is a positive regenerative capacity of young beech trees. Over the same period more annual shoots were healed in comparison with those that were newly infected (10.8% on stems and 0.6% on branches).

Liquid or frozen storage of poultry semen negatively affects the sperm motility and has crucial role in reducing fertility. Phospholipids in chicken sperm membrane are composed of high proportions of polyunsaturated fatty acids which are susceptible to lipid peroxidation. One of possible ways to improve semen quality is supplementing the ration with antioxidant compounds such as melatonin. In this study, seventy-two roosters were randomly divided into three equal groups. The first group (control; Group C) was exposed to 14 h light and 10 h darkness. The second group (Group M) was exposed to the same lighting period, but in combination with melatonion supplementation (3 mg/kg body weight daily). The third group (Group L) was exposed to 24 h constant light. Semen was collected using abdominal massage and stored for 6 and 12 h at 5°C. Motility, viability, malondialdehyde (MDA) concentration, fertility, and hatchability were evaluated before and after storage. The results showed that the percentage of viable and motile sperms was significantly (P < 0.05) decreased in the control samples after 6 h storage but in Group M these parameters were significantly (P < 0.05) decreased after 12 h. The MDA concentration was significantly (P < 0.05) decreased in Group M compared with Group C after 6 and 12 h storage. The sperm membrane lipid analysis showed that the percentage of unsaturated fatty acids in Group M was significantly (P < 0.05) lower than in Group C. Fertility and hatchability did not change significantly (P < 0.05) both in M and L Groups compared with C Group. Melatonin administration improved semen quality and decreased lipid peroxidation during liquid storage. It also reduced the percentage of polyunsaturated fatty acids in the sperm membrane lipid composition but it did not affect fertility and hatchability.