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The differences between oak lepidopteran communities were studied in Slovakia in 1993 and 1994. Sampling was undertaken between April and October on 3 oak species (Quercus robur, Q. petraea, Q. rubra). Biological traits of larvae were examined in order to explain differences in seasonal guilds among oaks. Communities varied in structure and abundance. Species richness in four seasonal guilds (flush, late spring, summer and autumn feeders) had a similar pattern on each of the studied oak species. The guild of flush feeders had the richest species assemblage, followed by the late spring feeder guild and both guilds were significantly richer than the summer feeder guild and autumn feeder guild.

The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd²⁺-enriched medium) and rye flour was performed by SEC/ICP-MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1-2 kDa fraction. Molybdenum occurred in all samples in the fraction of 3 kDa. During five days of cultivation in a solution of 30 µmol/l Cd²⁺, the plants accumulated as much as 5 mg/kg fresh matter of Cd, but its soluble portion represented only 12-15%. The prevailing portion of Cd complexes was contained in the fraction of 3 kDa, while the minor part occurred in the fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of the extracts of all samples (i.e. those of 1-2 kDa) were refined by immobilised metal affinity chromatography. The isolated ligands of trace elements were peptides rich in dicarboxylic aminoacids.

Pears cv. Pituralka are autochthon old cultivar from west Slovenian region and northern part of Italy, that were traditionally consumed as cooked or baked meals. In our study we developed new products and defined their nutritional value. Different thermical treatments (cooking and baking) were used for preservation of these products in sugar solution with or without an ascorbic or citric acid and packed in vacuum in a thermostable polyamide/polypropylene (PA/PP) foil. The products made from pears cv. Pituralka were sensory evaluated. The results show that Pituralka pears can be consumed only if they are thermally processed. Containing high amount of total fiber (6-7 g/100 g) products have functional perspective as prebiotics. Having low acidity level (total amount of acids 12.9-15.3 mg/100 g) products from pears cv. Pituralka have very acceptable sensory properties, especially for people with dyspepsia. However low amount of vitamin C (0.5 mg/100 g) has affect on higher sensitivity to oxidation, that needs to be taken into consideration during processing and storage.

During the years 2006 and 2007 the phytoextraction ability of maize (Zea mays), willow-tree (Salix smithiana) and poplar (Populus nigra × P. maximowiczii) to accumulate cadmium, copper, mercury and zinc was investigated. Small scale field experiment was carried out on soil contaminated with chemicals from the waste incineration plant in Hradec Kralové (Czech Republic). Screening of this allotment showed very different contamination of all observed risk elements on places where the material intended to be burnt without safety of leakage into soil. Grown plants showed the different accumulation of observed elements in plant tissues as well as the influence of total content of the risk elements in soil. The highest Cd (1.5-1.73 mg/kg) and Zn (242-268 mg/kg) concentrations were found in willow-tree biomass mainly in the leaves. Cu and Hg were mostly accumulated by maize roots (14.6-15.8 mg Cu/kg and 1.3-7.4 mg Hg/kg) and lower amount was found out in willow-tree leaves again. In reference to total production of each plant the maximum Cd and Zn uptake by aboveground biomass was found in poplars (201 mg Cd/m² and 38 200 mg Zn/m²) and maize, which showed high Zn uptake. The biggest amount of copper (2563 mg Cu/m²) was accumulated by aboveground maize biomass on the collection point with the highest Cu concentration in soil and by poplar (2394 mg Cu/m²) on the other collection point. The highest Hg uptake differs in reference to total Hg content in soil; willow-tree has the highest uptake on the place with lower Hg content in soil (44.6 mg Hg/m²) and maize has the highest uptake on the place with higher Hg content in soil (92 mg Hg/m²).

In an experiment with 45 neonatal male Holstein calves, effects of yeast cultures Yea-Sacc^® 1026 (SC) containing Saccharomyces cerevisiae (strain 1026) and Vitex (KF) containing Kluyveromyces fragilis (strain Jürgensen) on rumen fermentation characteristics were determined. From day 4 to day 56 of age, the calves were allocated to one of the three dietary treatments (Control, SC, and KF) of 15 animals each, placed in individual pens, and fed 4 l of whole milk twice daily and a basal concentrate mixture ad libitum as calf starter feeds. The control treatment was not supplemented with yeast culture. The yeast culture supplements Yea-Sacc^® 1026 and Vitex were top-dressed at 10 g/calf daily on the basal concentrate mixture of treatments SC and KF, respectively. At the end of the experiment (day 56), all calves were slaughtered and the rumen fluid was analysed. The administration of yeast cultures Yea-Sacc^®026 and Vitex to calves did not affect final body weight (BW), BW gain, dry matter intake, feed conversion ratio, ruminal pH, lactic acid concentration and the molar proportion of propionic acid, but it decreased (P < 0.05) the total volatile fatty acid concentration and the molar proportion of butyric acid, and increased (P < 0.05) the molar proportion of acetic acid and the acetate to propionate ratio. In addition, the microbial cellulolytic activity was higher in calves that received both yeast cultures compared to the control treatment. The results of this study suggest that the ruminal fermentation was more stable in calves receiving yeast culture supplements.

In order to determine the environment carrying capacity in terms of biomass production utilizable by game 360 representative sample plots (1 × 1 m) were laid out in the growing season on the basis of typological classification in forest stand of an area of 1,796.49 ha in the studied region of the Soutok Game Preserve, Židlochovice Forest Enterprise, Lanžhot Forest District. On meadows, pastures and others areas producing grass and herbs of a total area of 532.87 ha, other 57 sample plots were laid out and sample of biomass utilizable by game. Quantification of the biomass was carried out on the basis of the area cover of grass and woody undergrowth. In total, forest and non-forest land provides 14,659,851 kg grass and herb utilizable biomass. In forest stands production was found of 6,826,662 kg grass and herb biomass (on average 380 g/m²) and on meadows and pastures 7,833,189 kg (on average 1,470 g/m²). Moreover, production of 1,401,262 kg (on average 78 g/m²). Laboratory analyses were carried out of naturally dried-up samples of biomass and these values available energy were ascertained: the energy of grass and herb biomass amounted to 5.7 MJ/kg, the utilizable energy of wooody origin amounted to 4.03 MJ/kg. In view of the standardized game population size up the available food supply sufficient, because the energy requirement was fully covered by their daily quantitative consumption of biomass.

The diversity of arbuscular mycorrhizal (AM) fungi of Acacia catechu Willd. was studied. Dominant AM spores, the bacterium Rhizobium sp. along with the fungus Trichoderma viride were isolated from the rhizosphere of A. catechu and mass-produced in laboratory. The co-inoculation effect of Glomus mosseae, Glomus fasciculatum, mixed AM (Glomus spp. [except G. mosseae, G. fasciculatum] with Acaulospora spp., Sclerocystis spp. and Gigaspora spp.), Rhizobium sp. and Trichoderma viride was studied as exerted on the growth of A. catechu seedlings. All inoculated seedlings showed improved seedling growth compared to the control. Inoculated seedlings had a pronounced effect on all growth parameters such as height, fresh and dry weight of roots and shoots, AM spore count, per cent mycorrhizal colonization in roots and root nodule number in comparison with uninoculated seedlings. Phosphorus uptake was also higher in inoculated seedlings than in the control. This study provides a good scope for commercially utilizing the efficient strains of AM fungi for beneficial effects with other beneficial rhizosphere microflora in the primary establishment of slow growing seedlings ensuring better survival and improved growth.

The paper focuses on the evaluation of the contemporary situation of two economic sectors in the frame of the European Union, namely the wholesale and retail of agricultural and food products. For these industries, the structural profile with the indicators of turnover, value added and employment was elaborated. Hereafter the costs, productivity and profitability indicators ranking of the top 5 Member States were compared to the EU-25 averages. After a 3.1% increase in the turnover index in 2000, the evolution of sales for the agricultural wholesaling sector recorded a series of small increases and decreases in the turnover, ranging from - 0.6% in 2002 to 1.5% in 2004. Between 2000 and 2005, the agricultural wholesaling sector generally recorded a slower rate of expansion for turnover than the wholesale trade average. The specialised in-store food retailing sector generated EUR 120.4 billion of turnover in 2003. The wealth creation of the specialised in-store food retailing was valued at EUR 23.8 billion; equivalent to 6.5% of the retail trade and repair total, while this activity employed 1.4 million persons, some 9.0% of the retail trade and repair workforce. Half (49.9%) of the turnover in the specialised in-store food retailing sector in the EU was generated by enterprises selling fruit, vegetables, meat, fish or bakery products.

The paper presents the results of the research focused on crisis management, which is based on the process of change management by the author-formulated methodology of the creation, implementation and changes of business strategies and requirements for their improvement due to the incidence of a turbulent management environment and other essential factors. The main factors of crisis and methods of their analysis are formulated. Thereinafter, the strategies of solving crisis situations are formulated. The company Bioveta, Inc. is analysed in detail. The research has proved that companies with modern management need a well-made and working system of crisis management due to their complexity and risks.

The production of organic acids and changes during decomposition of rice straw amended with tricalcium phosphate (TCP) and Udaipur rock phosphate (URP) were studied under laboratory conditions. The organic C content of rice straw decreased and total N increased with time, resulting in a decrease in C:N ratio as the decomposition progressed. The pH decreased to acidic range in all the treatments on day 15, but became alkaline again later on. Soluble P increased at 15 days after incubation, declined later during decomposition, and was highest in the treatments containing TCP and Aspergillus awamori inoculation. Citric, oxalic, formic and maleic acids were detected during decomposition of rice straw and maximum amounts were present on day 15. Citric and oxalic acids were responsible mainly for P solubilization from TCP and URP; generally, citric acid was the most effective in P solubilization.

The objective of this study was to investigate the capability of radish to extract lead from soils contaminated with lead resulting from air pollution. A randomized block experiment design was performed. The soil was contaminated with PbNO₃ and the treatments consisted of 180 (standard), 250, 350, 450, 800 and 1000 mg/kg lead. After development, plants were harvested and divided into shoots and roots. The lead content of each plant part as well as the soil-lead were measured. The results indicated a non-linear positive relation between the lead concentrations in soil and that accumulated in plant roots and shoots. By increasing the lead concentration in soil, its accumulation in plant tissues was also increased. Most of the extracted lead was accumulated in the roots (208.1 mg/kg) compared to shoots (27.25 mg/kg). Since radish can be seeded up to five times a year, and its yield may reach up to 20 t/ha, it can be used to remediate lead-polluted topsoils (0-10 cm).

Actual yields of maize harvested by farmers are at level much below attainable yield potential of currently cultivated varieties. Among many growth factors zinc was recognized as one of main limiting factors of maize crop growth and yielding. This hypothesis has been verified within a three-year field study, where zinc fertilizer was applied to maize plants at the 5^th leaf stage. Maize crop responded significantly to zinc foliar application in two of three years of study. The optimal rate of zinc foliar spray for achieving significant grain yield response was in the range from 1.0 to 1.5 kg Zn/ha. Grain yield increase was circa 18% (mean of three years) as compared to the treatment fertilized only with NPK. Plants fertilized with 1.0 kg Zn/ha significantly increased both total N uptake and grain yield. Yield forming effect of zinc fertilizer revealed via improvement of yield structure elements. The number of kernels per plant showed the highest response (+17.8% as compared to the NPK plot) and simultaneously the highest dependence on N uptake (R² = 0.79). For this particular zinc treatment, however, the length of cob can also be applied as a component of yield structure significantly shaping the final grain yield.

Forty samples of grass silages, made from the five most widely used grass species in ruminant nutrition (Dactylis glomerata L.,Phleum pratense L., Lolium perenne L., Festuca arundinacea L. and hybrid Felina) were tested in the present study. These grass species conserved by an ensiling process were compared among each other according to dry matter (DM) rumen degradability parameters (a = portion of DM solubilized at initiation of incubation, b = fraction of DM potentially degradable in the rumen, c = rate constant of disappearance of fraction b and ED_DM = effective degradability of DM, estimated for each ingredient assuming the rumen solid outflow rates of 0.02 (ED_DM2), 0.05 (ED_DM5) and 0.08 (ED_DM8) h^-1). Based on the chemical composition of grass silages the regression equations for prediction of ED_DM were evaluated. The influence of the ensiling process on dry matter degradability parameters was also assessed. The best values of ED_DM were determined for Lolium perenne (ED_DM2 = 753.2, ED_DM5 = 631.1 and ED_DM8 = 567.7 g/kg DM). The best predictor was NDF (R²-values of 0.757 (ED_DM2), 0.863 (ED_DM5) and 0.906 (ED_DM8)). Using two predictors the accuracy level increased. The combination of CF and NDF gave R²-values 0.892, 0.920 and 0.929 for ED_DM2, ED_DM5 and ED_DM8, respectively. The regression equations based on the most important grass species harvested in different vegetation periods seem to be a useful tool for practical use. No significant P < 0.05) effect of the ensiling process in relation to dry matter rumen degradability parameters was proved.

The basis of the biogas production in agriculture is the processing of waste agricultural products (particularly excrements of farm animals but also phytomass). Different but rather similar is the biogas production from biologically degradable municipal waste (BDMW) and biologically degradable industrial waste (BDIW) coming mainly from food industry. The processing of these wastes in agricultural biogas stations could significantly improve their economy. It is necessary to note that all these biogas stations differ from the wastewater cleaning plants where municipal sludge water from public sewers is processed. The municipal sludge water processing to biogas by anaerobic fermentation is a classical technology introduced all over the world. At present, about 100 wastewater cleaning plants operate in the Czech Republic using regular sludge processing into biogas. Electricity produced is utilised mainly for the needs of own operation of waste water treatment plant (WWTP), partly it is sold into public power net. The heat energy is used for heating in the process and its surplus is utilised for operational and administrative facilities. Usually, the heat and electricity quantities produced do not cover the wastewater cleaning plant operation. Agricultural biogas stations and biogas stations for BDMW processing provide considerably higher gas yields because they work with higher dry matter contents in substratum, i.e. 8-12% (compared with waste water treatment plants - 2-6%), and are able to produce high gas surpluses for following applications. Frequently discussed issue are the processing of slaughter waste and grass (or public green areas at biogas stations).

The study is a follow-up of the production potential of Douglas fir in mesotrophic sites of the Křtiny Training Forest Enterprise (TFE) (Journal of Forest Science, No. 7, 2008). Production parameters (height, dbh, volume) of Douglas fir are also evaluated, but in acid sites of the Hůrky Training Forest District, Secondary Forestry School in Písek in mature stands. In total, 17 mixed stands with the proportion of Douglas fir aged 88 to 121 years were assessed. Comparing 10 Douglas fir trees with 10 Norway spruce, Scots pine or European larch trees of the largest volume, higher and generally markedly higher production potential of the introduced Douglas fir was always found in all assessed stands. Groups where the volume of Douglas fir trees was two to three times higher than the volume of spruce, pine or larch were not an exception. For example, in stand 22B10, the mean volume of the 10 largest Douglas fir trees was 6.30 m³ but the volume of spruce trees was only 1.93 m³ and the volume of larch trees 2.25 m³. Differences between the mensurational parameters of Douglas fir and spruce (or larch) assessed by the ANOVA test were statistically highly significant. At present (based on annual ring analyses), the volume increment of particular Douglas fir trees ranges at level of 0.06 to 0.10 m³/year (i.e. about 0.6 m³ to 1.0 m³ per 10 years) in mature stands.

The article deals with the analysis of food market on the level of consumer prices structure, through the identification of margins in the selected food verticals and through the analysis of consumer behaviour associated with changes in prices and income of population. The article also discusses the factors which may affect the current growing trend in prices of food in the SR and worldwide and subsequently, the reduction of gap between the Slovak and the EU prices.

The absence of good agricultural and manufacturing practices in the production and postharvest handling of fresh produce, such as green asparagus or green onions increase the contamination risk by biological hazards like Salmonella. The objective of this work was to investigate the efficacy of chlorine (200 and 250 ppm), hydrogen peroxide (1.5% and 2%), and lactic acid (1.5% and 2%) sanitisers during different exposure times (40, 60, and 90 s) on the reduction of Salmonella enterica subspecie enterica serovar Typhimurium in inoculated fresh green asparagus and green onions. Washing with clean water only reduced < 1 log10 CFU/g in both vegetables. The most effective sanitiser evaluated for fresh green asparagus and green onions disinfection appeared to be 2% lactic acid reducing Salmonella growth close to 3 log₁₀ CFU/g. Hydrogen peroxide was the least effective agent for Salmonella Typhimurium reduction. No effect was observed of the exposure time of inoculated product to sanitiser up to 90 seconds. These results confirm that lactic acid could be used as an alternative for fresh green asparagus and green onions sanitation.

Within the framework of the research project ELISA methods for the quantitative determination of allergenic substances in foodstuff and raw materials were developed. ELISA kits for allergenic proteins of milk (casein, beta-lactoglobulin and BSA) egg white proteins and mustard proteins were validated and collaborative studies were performed to prove the validation of the ELISA methods developed. Various methods of extraction were tested. The parameters as a limit of detection, as a limit of quantification, robustness, repeatability and accuracy were determined. A broad range of zero matrices for allergens were tested as well. The ELISA kits are suitable for the determination of allergens according to EU legislation Directive 2005/26/EC and Directive 2006/142/EC in the laboratories focused on this topic.

Net N mineralization rate (NMR), net N consumption rate (NCR), microbial biomass carbon (MBC) and nitrogen (MBN), potentially mineralizable N (PMN) and mineral N (N-NH⁺₄ and N-NO^-₃) were measured in paddy soil at five growth stages of rice to determine the effect of long-term fertilization in subtropical China. The studied long-term treatments included CK (no fertilization), N, NP, NPK and NPK + OM (NPK plus organic manure). The NPK + OM treatment gave the highest values of the measured variables among all treatments. There was no significant difference in other treatments except for mineral N and PMN at early growth stages. All these variables were generally highest at transplanting stage as two thirds of fertilization was applied as basal fertilizers and the rice uptake was low. Then they decreased or leveled off with the rice growth stages except for MN in all treatments. Stepwise regression revealed that NMR was significantly correlated with MBC and N-NH⁺₄ (R² = 0.954, P < 0.01) at all rice growth stages. So, mineral plus manure fertilizer application and more mineral fertilizer as topdressing were recommended in subtropical paddy soil.

The seeds of two medicinal plants, namely Chamomilla recutita (Babong) and Origanum majorana (Bardaqoush) were subjected to germination in different NaCl concentrations, polyamines (putrescine, spermidine, spermine) and to combination of both. The results revealed that the growth alterations induced by NaCl were alleviated by various levels of polyamines. The organic solutes of both plant seedlings exhibited somewhat variable responses to various salinity levels or polyamines treatments and in combination of both treatments. Putrescine in Ch. recutita seedlings was more effective in alleviating the stress effects of salinization than spermidine and spermine, while in O. majorana seedlings spermidine was more effective. Generally, the degree of stimulation differed according to the type, concentration of the additive used and the type of the plant tested.

The elimination of Plum pox virus (PPV) in different stone fruit cultivars was verified by the method of thermotherapy in vivo. Trees of two plum cultivars Čačanská lepotica and Švestka domácí, apricot cultivars Leskora and Velkopavlovická, and peach cultivars Redhaven and Earliglo were used. They were infected artificially with two strains of the virus (PPV-D, PPV-M). Two cycles of thermotherapy in vivo were performed. During the first cycle, 16 trees of plum, apricot and peach were treated for 15 days at 37°C. In the second thermotherapy cycle, 10 trees of individual cultivars of plum, apricot and peach were treated for 22 days at 37°C. In the first thermotherapy (T1), 8 trees out of 16 died; PPV was eliminated in 2 trees of cv. Čačanská lepotica, 1 tree of cv. Švestka domácí and 2 trees of cv. Velkopavlovická. In the second thermotherapy (T2), 1 of 10 treated trees died. The virus was eliminated in 2 trees of cv. Čačanská lepotica, 1 tree of cv. Leskora, 2 trees of cv. Velkopavlovická, and 1 tree of cv. Redhaven. Nine (T1) and seven (T2) months after the thermotherapy, the presence of PPV was detected in 6 out of 11 originally recovered trees using ELISA. Out of 26 trees, 4 trees remained recovered: 2 plum trees and 2 apricot trees. One of these trees, apricot cv. Leskora was originally infected with PPV-M strain, whereas the other three with PPV-D strain. None of the 10 peach trees was treated successfully.

In this study, the circulus arteriosus cerebri of the ground squirrel (Spermophilus citellus) was investigated. Five ground squirrels were used as subjects. Coloured latex was injected from the left ventriculi of the hearts of all the squirrels. When the vertebral arteries of two of the animals were ligatured, it was found that there was no internal carotid artery. After careful dissection, the circulus arteriosus cerebri (the circle of Willis) was investigated. The right and left vertebral arteries gave rise to the caudal cerebellar artery before forming the basilar artery. The basilar artery formed the caudal communicans artery that was the caudal part of the circulus arteriosus cerebri on the pontocrural groove (sulcus pontocruralis). The caudal, medial, rostral cerebellar, the common root formed by the caudal cerebral and choroid arteries, the rostral choroid, the rostral and medial cerebral arteries arose from the vertebral, basilar and caudal communicans arteries and dispersed to the cerebrum and cerebellum from caudal to cranial. The termination and the branches of the rostral cerebral artery in ground squirrels varied. It was observed that the internal carotid artery does not supply the circulus arteriosus cerebri in ground squirrels.

The study deals with the monitoring of dissolved organic carbon (DOC) concentrations in seepage water sampled from differently managed forest plots in the Drahanská vrchovina Upland. Simultaneously, the input of DOC in precipitation and throughfall is evaluated. Preliminary results show higher mobility level of carbon substances in forest soil in a pure spruce stand compared to mixed stand or a pure beech stand. DOC can be one of suitable characteristics to evaluate the conversion effectiveness of spruce monocultures.

The aim of this paper is to show some empirical evidence of the sustainability and decision making in dairy production in Turkey. The main material of the study was obtained from the data collected by the survey conducted in the villages in Merkez, Manavgat and Serik districts of the Antalya province. Within the context of the research, 75 farmers were interviewed in the 2007 production season. The research results indicate that the households are the most important decision maker in terms of farming decisions. 56.3% of the farmers in the small farms, 61.5% of the farmers in the medium-size farms and 41.2% of the farmers in the large farm noted that they could quit dairy production activity if they received a fixed salary.

The quality of water has to be controlled and monitored by drinking water suppliers during all stages of the treatment process from the water sources to the end of distribution systems. The research, performed in Czech Republic from 2006 to 2008, deals with the assessment of the affect of water tanks on the quality of water supplied to consumers, specifically from various points of view: microbiological, biological and physic-chemical changes in water accumulation. Also studied was the influence of the air on the quality of accumulated water (secondary contamination), the influence of the structural layout and hydraulic ratios. In the project quick screening methods (paddle testers and BART^TM tests) were applied in the collection of water samples and scrapings from wetted surfaces of water tanks. The results of the contamination degree discovered in the course of the project solution will serve as basic data for a scale that should evaluate the degree of water tank pollution as well as for resulting corrective measures or optimisation of water tank cleaning. The recommendations of limits for a scraping sample are based especially on the microbiological parameters. Secondary air contamination plays an important role in maintains of biologically stable water. Based on the number of microbial contamination discovered water tanks will be categorised and methods of suitable measures to be taken will be stipulated, operation optimisation as well as cleaning (schedule, methods and frequency of cleaning). The water quality in a storage tanks depends on their maintenance, e.g., to prevent the plaster falling on water surface, the use of antifungal surface coatings (prevention the growth of fungi on walls), the use of ceramics surface of reservoir walls, dark conditions (no windows or blue sheets) in all technological units, the prevention of dust fall out, the selection of suitable air condition and special air filters.

Industrial wine yeasts Saccharomyces cerevisiae Syrena, an interspecies hybrid (S. cerevisiae × S. bayanus) HW2-3 and Schizosaccharomyces pombe met 3-15 h^{+>/sup> were examined to determine changes in fermentation profiles in different environmental conditions in YG medium with different concentrations of glucose (2, 6, 40 or 100 g/l), L-malic acid (4, 7 or 11 g/l) and at pH 3.0, 3.5 and 5.0. The results were obtained by HPLC method (organic acids, acetaldehyde, glycerol, diacetyl) and enzymatically (L-malic acid, ethanol). In anaerobic conditions (100 g/l glucose), the optimal parameters for L-malic acid decomposition for S. cerevisiae Syrena and the hybrid HW2-3 were 11 g/l L-malic acid and pH 3.0 and 3.5, respectively. S. pombe expressed the highest demalication activity at 40 and 100 g/l glucose, 7 g/l L-malic acid and pH 3.0. The fermentation profiles of selected metabolites of yeast were unique for specific industrial strains. These profiles may help in the proper selection of yeast strains to fermentation and make it possible to predict the organoleptic changes in the course of fruit must fermentation.}

Citrulline has been recently shown to behave as a novel compatible solute in the Citrullus lanatus (Cucurbitaceae) growing under desert conditions. In the present study we have investigated some aspects of the relationship which might occur in leaves of melon seedlings, also known to produce citrulline, between the capacity to accumulate this ureido amino acid and salt tolerance. With this end in view, salt-induced changes at the citrulline level have been compared in two melon genotypes exhibiting contrasted abilities to withstand the damaging effects of high salinity. Progressive salinization of the growing solution occurred at 23 days after sowing. The final 250 mmol/l external NaCl concentration was reached within 5 days and further maintained for 16 days. In response to this treatment, it was found that the citrulline amount increased in fully expanded leaves of both genotypes according to different kinetics. The salt tolerant genotype Midyat was induced to accumulate citrulline 4 days before the salt sensitive Yuva and as a consequence the final amount of this amino acid was twice higher in the former than in the latter. Compared with citrulline, the free proline level was found to be relatively low and the changes induced in response to the salt treatment exhibited different trends according to the genotypes under study. Thus at the end of the treatment mature leaves of the salt sensitive Yuva contained higher amount of proline than those of Midyat. The changes in the calculated molar ratio between citrulline and free proline suggested that salt tolerance might be associated with high values for this ratio and vice et versa for sensitivity. The interest of citrulline as a biochemical marker for salt tolerance of melon genotypes is discussed.

The effects of putrescine (a polyamine), low temperature and their combinations on the activities of apoplastic antioxidant enzymes were studied in the leaves of two wheat cultivars, winter (Dogu-88) and spring (Gerek-79). Fifteen-day-old wheat seedlings were treated with putrescine solutions (0.1, 1 and 10mM) prior to cold treatment (5/3°C). The activities of apoplastic catalase, peroxidase and superoxide dismutase were determined in the leaves both under normal and cold conditions at 1, 3 and 5 days. The results indicate that cold treatment significantly increased the activities of apoplastic catalase, peroxidase and superoxide dismutase in winter wheat while not generally affecting spring wheat. Under control conditions, the putrescine treatments were more effective in increasing the enzyme activities in winter wheat than in spring wheat. However, under cold conditions, the putrescine treatments surprisingly induced enzyme activities in spring wheat while generally reducing those in winter wheat leaves. The results show that putrescine may act as an agent inducing primary changes in the apoplastic antioxidant system of wheat leaves during reactive oxygen species-mediated damage caused by low temperature stress.

The effect of egg collection time, genotype and housing system on egg quality characteristics was evaluated in an experiment with laying hens of ISA Brown, Hisex Brown and Moravia housed on litter and in conventional cages. The experiment was conducted from 20 to 64 weeks of age. Eggs were collected and recorded daily at 06:00, 10:00 and 14:00 h. Once every four weeks, two days in row, all eggs laid from each pen or cage at each oviposition time were used for egg quality analyses (total 1 694 eggs). Egg collection time was dependent on genotype. Significantly more eggs were laid at 06:00h by ISA Brown while Moravia laid eggs mainly at 10:00 and 14:00 h. Housing on litter postponed the time of oviposition. The highest egg weight (62.09 g) was recorded in Hisex Brown genotype placed in cages and at 06.00 h. Interactions of all factors were determined also in Haugh units (P ≤ 0.041). Eggshell strength was affected by housing system, genotype and egg collection time (P ≤ 0.049). It was higher in cages than on litter, and lower in the Moravia genotype in comparison with the other two strains.

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of F_ST (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.