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The paper is focused on the problems of income disparity in agriculture. This economic as well as social phenomenon is often discussed, especially at the administrative level, nevertheless, it has not been exactly defined and methodically delimited yet. The comparison of average wages of farmers with average wages in inhomogeneous industry and with average wages in the very sector-differentiated national economy used today can be considered as problematic. In the paper, the possible system of income disparity monitoring is suggested which would remove the current deficiencies. In its frame, a special attention is paid to regional aspects of income disparity and the relation of wages and labour productivity.

Three hundred and thirty-one (331) horses out of 371 cases (i.e. 351 individuals discharged from the clinic) were followed up in order to evaluate long-term survival rate and complications after colic surgery. Twenty horses were subjected to surgery twice during the period of this study. Out of the sample of 331 patients, 302 animals (91.2%) survived at least 11 months after the operation. Twenty-nine horses (8.8%) died or had to be euthanized. Colic episode was considered to be the cause of death in 23 (79.3%) out of 29 patients. Seven colic patients had to be reoperated. The remaining six patients died of a cause unrelated to the gastrointestinal disease. Seven out of 302 long-term surviving patients needed the second operation within the first 11 months after the first surgery. Colic episode was reoperated in another 15 patients after 12 months to 4 years after the first operation. Conservative treatment was necessary in another 10 long-term surviving horses. In eight long-term surviving horses and in one patient that did not survive 11 months, incision hernias were identified as the postoperative complication. Twenty-one horses out of 331 were subjected to relaparotomy during their first hospitalization. Eighteen out of these (85.7%) survived 11 months after the surgery; the remaining three were euthanized within six months due to colic.

The genetic diversity of 140 accessions of Triticum turgidum ssp. carthlicum Nevski em. A. Löve & D. Löve and 159 accessions of T. turgidum ssp. polonicum L. em. Thell. was evaluated by the analysis of HMW glutenin subunits. Seven allelic variants were found among the carthlicum accessions: three at the Glu-A1 locus (two of them were novel alleles) and four at the Glu-B1 locus (one of them novel). More variability was found among the polonicum accessions with 16 allelic variants: six at the Glu-A1 locus (three of them novel), and ten at the Glu-B1 locus (five of them novel). Totally, ten new alleles were found, one of which appeared in both subspecies. Out of 19 different combinations of alleles detected in both subspecies, 14 were novel. Based on the available passport data, the carthlicum accessions could be separated by origin into 18 groups, and the polonicum accessions into 33 such groups. The genetic diversity was lower among the carthlicum (Ht = 0.174) than among the polonicum accessions (Ht = 0.562). In both subspecies, most diversity was present between groups differing in origin, whereas diversity within the groups was very low. The detected variability offers possibilities for the improvement of bread making quality in durum wheat through introduction of newly detected alleles and for the broadening of genetic diversity in this wheat species.

The first part of the study concerns the effect of the stage of maturity on nutrient content and leaf percentage of lucerne (Medicago sativa). The plants of lucerne were harvested and analysed at several stages of growth. The crop yield and changes in the concentration of nutrients were determined. The leaf percentage from whole herbage and leaf yield were determined as well. The leaf percentage at the growth stage of small buds was significantly (P < 0.05) higher than at the growth stage of bloom in all three cuts (1^st cut 52.7% vs. 46.62%; 2^nd cut 52.03% vs. 44.70%; 3^rd cut 50.58% vs. 46.26%). Crude protein content of lucerne was decreased significantly (P < 0.05) from the large bud growth stage (small buds 219.6 g/kg DM and large buds 203.1 g/kg DM vs. bloom 173.5 and after bloom 154.2). In the second part of the study, the effect of silage additives on fermentation characteristics of lucerne was investigated. The bacterial inoculant (containing homo- and heterofermentative lactic acid bacteria), chemical additive (containing formic acid, propionic acid, ammonium formate and benzoic acid) and the bacterial inoculant with benzoic acid were used for the improvement of fermentation process.The addition of the inoculant with L. buchneri increased acetic acid (P < 0.05) concentration compared to the silage with chemical additive (group I with inoculant - 1.22%; group Ch with chemical additive - 0.84%; group ICh with chemical additive and inoculant - 1.43). Control silage (C) without additive contained 1.14% acetic acid in dry matter.

More than 1,300 seedlings of European plum originating from crossing combinations with at least one parent showing hypersensitivity resistance against PPV were analyzed for their reaction to artificial inoculation with PPV using the double grafting method with virus infected interstem. It was shown that the hypersensitivity resistance against the virus is a phenotypically quantitative trait. The different kinds of symptoms observed in the test system, which contribute to the hypersensitivity resistance, range from weak necrosis on the leaf blade and on the stem to the death of the whole young shoots. A hypersensitivity index was developed which helps to determine the degree of hypersensitivity resistance of an individual genotype. Its use is strongly recommended as selection tool in breeding for hypersensitivity resistance.

The Pinus mugo complex (P. mugo Turra sensu lato, and/or species aggregate or group - P. mugo agg., including its internal as well as external aggregate hybrids) is an important fragment of European dendroflora. For its high variability this complicated group has not been solved in taxonomical terms satisfactorily yet. Most problems are mainly caused by a large amount of taxa of uncertain value described in the past, which often more or less overlap or are based on insufficiently researched and unsuitably interpreted characters. Such facts coincidently cause further problems in applied disciplines. Specialized European literature already contains a large amount of information referring to individual taxa of this complex. Much information is listed under various, often commonly not known or not used scientific names. In order to make the information available and useful, it was necessary to collect the most frequent names used in the past and to assort them in a tabular manner into a convenient synoptic schedule respecting the present concepts of the complex and assign them with the present most commonly used names.

The paper characterizes the natural conditions and current state of floodplain forests in the area of Litovelské Pomoraví and proposes the protective management of the area in accordance with the European Natura 2000 system. The paper describes the geographical location and the natural conditions of the area of interest (climate, geology, geomorphology, hydrology and flood regime, soils, and vegetation). Six groups of geobiocene types were identified, classified and described in detail. Assessment of the ecological stability of the forest stand was carried out using biogeographical differentiation of the landscape and the outline of the forest ecosystem management is drawn based on the differentiation of the protected landscape area into zones with various levels of protection.

The dynamics and forming of European agriculture are determined by many considerably heterogenous and complicated processes and trends which influence mutually and moreover they work in a different way in developed and developing countries. An attention will be paid to basic global trends, the role of the Common Agricultural Policy, the influence of agrarian markets, the promotion of multifunctional agriculture etc.

The agricultural production in less favoured areas (LFA) should, first of all, fulfil the non-production goals, as is the nature and environment protection and job opportunities generation. These goals could be achieved within the conditions of sustainable agricultural production systems. This paper is aimed on the analysis of how the companies operating in marginal conditions realize commercial principles and targets specified for the LFA conditions. The obtained knowledge indicates that the managers of majority of the companies do not respect the recommendations and persist in the intensive production in spite of the unfavourable conditions.

The aim of the study was to verify under experimental conditions the hypothesis that a reliable protection of piglets against Actinobacillus pleuropneumoniae (App) infection can be obtained by colostrum-derived antibodies and/or a low-dose infection from sows naturally infected with App. Twenty-eight piglets were allocated into four groups. Piglets of groups 1A and 1B were the progeny of App-seronegative sows, piglets of groups 2A and 2B originated from App-seropositive sows. At the age of 4 weeks, piglets in groups 1A and 2A were exposed to a low infection dose with the pathogenic strain of App serotype 9 (4 × 10⁴ CFU/ml). At the age of 8 weeks, all four groups of piglets were exposed to the App serotype 9 challenge (2.5 × 10⁷ CFU/ml). We compared the differences in clinical, radiological, pathological changes and cytological findings in blood and bronchoalveolar lavage samples between groups with different levels of antigen-specific immunity after challenge. Piglets that obtained colostrum-derived antibodies and experienced a low-dose App infection were best protected against App challenge. More apparent clinical and pathological changes were observed in groups protected with either only colostrum-derived antibodies or active immunity induced by a preceding low-dose infection. However, none of the types of protection prevented developing of the disease and characteristic changes in the lungs. Cell counts changes in blood and lavage gave evidence of only bacterial infection in progress; however, between-group differences were not marked. Antibodies (IgG, IgA) present in the respiratory tract at the time of challenge played a major role in protection against App infection.

Malachite green has been used as an effective compound to control external fungal and protozoan infections of fish since 1933 but it has never been registered as a veterinary drug for use in food fish because of its potential carcinogenicity, mutagenicity and teratogenicity in mammals. The present paper reviews negative side-effects of malachite green including its accumulation and persistence in fish that have been treated and describes other alternative substances for the treatment of fish and fish eggs.

The review focuses on recent progress in the breeding of small grain cereals (barley, wheat, oats) for resistance to the barley yellow dwarf virus (BYDV). First, the symptomatology of barley yellow dwarf (BYD) disease is briefly described and the genome of BYDV, its serotypes and mechanisms of its replication and translation in host plants are characterized. Great attention is paid to the description of resistance genes and sources of BYDV resistance that are currently used in some breeding programmes of barley, wheat and oats. In barley, the introduction of the Ryd2 gene into high-yielding cultivars is still desirable. An example of recent success reached in a European programme aimed at a pyramiding of resistance genes is the registration of the Italian feeding barley cultivar Doria, carrying resistance genes Ryd2, rym4 and Rdg1. The release of this cultivar resulted from the cooperation between EICR, Fiorenzuola d'Arda and CRI in Prague-Ruzyně in the field of virus resistance. Finally, some experiments employing transgenic techniques in the construction of resistant plants are mentioned. In conclusion, the advantages and disadvantages of classical breeding methods using crossing and transgenic techniques are compared and newly arising approaches are discussed.

A three-year field experiment was conducted from 2000 to 2002 in North-East Poland. Each year three sulphur fertilization rates in the form of sulphate (S- SO₄^2-) and pure sulphur (S-S⁰) were applied: 40, 80 and 120 kg/ha. The most beneficial effect on the yields of cabbage, onion and barley was produced by the rates of 40 and 80 kg S/ha, while the dose of 120 kg S/ha (especially when applied as S-SO₄^2-) reduced the yields of these crops. Increasing rates of sulphur used as a fertilizer caused increased concentration of sulphates in plants up to their luxury accumulation. Irrespective of the test crop species or form of sulphur applied, once the crops terminated their vegetative season, the plants fertilized with sulphur typically contained more total N than those fertilized only with NPK. The S-SO₄^2- fertilization tended to raise the accumulation of N-NO₃^- in the crops, especially during the juvenile phase. The application of 120 kg S-SO₄^2-) /kg caused depressed amounts of potassium in cabbage, onion and in barley during the heading phase.

The efficiency of embryo recovery in a superovulatory treatment was studied by perfusing the oviduct or the uterine horn in 3.5 and 7 days after sponge withdrawal, respectively. Eighty-four and seventy ewes of Aragonesa breed were used as embryo donors and receptors, respectively. The donors were distributed in 3 replications. The oestrus was synchronized with the insertion of FGA intravaginal sponges (30 mg) for 13 days. Six intramuscular injections of 18 mg pFSH were applied in decreasing doses at 12 hour intervals starting 48 hours after sponge withdrawal. The recovery rate (RR) (83.2 vs. 75.8%), the viability rate (VR) (73.5 vs. 47.2%) and the number of viable embryos (VE) per donor ewe (5.9 ± 0.79 vs. 3.0 ± 0.37) were higher (P < 0.01) in ewes whose perfusion was done directly in oviducts. The interval from sponge withdrawal to oestrus had no influence on RR in any groups; however it had an effect on the VR in 3.5 and 7 days old embryos. Embryos from ewes showing the oestrus within 20 hours after sponge withdrawal had a higher viability. On the other hand, using morphologic criteria, 26.5% and 52.7% of 3.5 and 7 days old embryos, respectively, were discarded before transfer. The percentage of ewes in each group that became pregnant was similar in both groups. We conclude that in superovulated ewes the embryo recovery rate, viability and number of viable embryos are higher in embryos recovered from the oviduct compared to those recovered from the uterus, without decreasing the conception rate and the viability rate is higher in ewes that show the oestrus 20 hours before sponge withdrawal.

The results of research on the value production of the stands of poplar clones Robusta and I-214 carried out in Slovakia are presented. Models of value yield tables were constructed separately for each clone. The models simulate gross and net financial yield of wood production in dependence on the site index and age of stand. They were constructed on the basis of the models of assortment yield tables, timber prices according to assortments and the models of own costs of timber felling and processing. The clone I-214 produces a faster and higher proportion of thicker assortments of average and below-average quality, and therefore it has the higher value production at a younger age only. Robusta produces smaller diameter but higher quality assortments and has the higher value production only at an older age. The site index of the stand is the most important factor in the value production of poplar clones. Differences in the production between site indexes are much greater than between the clones.

The aim of the study was to assess terrestrial ecosystem development (mainly vegetation and soil characteristics) in the area of a sand mine cast (located in southern Poland) that has been either reclaimed or left for natural succession. A total of 20 sites in a chronosequence of 5, 17, 20 and 25 years were set up in two site categories: reclaimed and non-reclaimed sites. Selected properties of initial soils and features of vegetation were measured and they included carbon accumulation in soil; biomass and diversity of communities were also estimated. Next, based on carbon accumulation, the energy trapped in ecosystem components was estimated. Although the results of plant community investigation did not show the same distinct differences between site categories, the case study suggests that reclamation significantly accelerates ecosystem development. In comparison with spontaneous succession, the complete forest reclamation was found to increase the amount of carbon accumulation, thickness of humus horizon, and energy trapped in soil organic carbon and plant biomass in the developing ecosystem 2-3 times and nitrogen accumulation 5 times.

The power function was used to show the dependence of gross, final and sold output on the gross value of total fixed assets and on the ratios of this value to the productivity of these three production categories. Further elaboration included the characteristics of variable features. The system of independent variables employed in the study allowed for the estimation of both extensive and intensive utilisation of fixed assets in Polish agriculture from 2002-2005. The study showed the diminishing impact of the productivity of fixed assets and the decreasing productive efficiency of Polish agriculture from 2002-2005. This situation was caused by the relative stability in the generic structure of fixed assets and by a slow average annual rate of increase in new fixed assets (8.74%).

Two cases of botulism in horses are described in the article. In the first case two horses died, one survived and recovered after four weeks. Botulotoxin type B was detected using a mouse bioassay in the gastrointestinal content of both dead horses; Clostridium botulinum bacteria were cultivated from one of them. In the second case two horses were affected. One of them was euthanized because of persistent recumbency, the second one recovered after six weeks. Detection of botulotoxin in the serum of the dead horse using the mouse bioassay was not successful.

Fluorescent Maillard compounds measurement provides more specific information on the extent of the Maillard reaction than other unspecific tools to monitor the reaction, and is suitable, as the first approach, to assess the nutritional quality of foods as related to protein damage. This work presents an optimised laboratory procedure for the measurement of total fluorescent intermediate compounds (FIC) associated with Maillard reaction, described and evaluated in a cereal-based product. Total FIC are evaluated using increased pronase E concentrations and different incubation times for the enzymatic hydrolysis, as well as three different sample clean-up steps after the enzymatic digestion. The effects of basic/acid media are considered for the stability of the fluorescent compounds. The standardised procedure is finally applied to breakfast cereals as a model of cereal-based products, analysing the correlation between total FIC production and fibre and protein contents. It is demonstrated that fluorescent compounds are mainly linked to the protein backbone in ready-to-eat breakfast cereals. Fluorescence measurement is presented as an inexpensive, rapid and accurate procedure to study the extent of Maillard reaction in breakfast cereals.

Otiorhynchus sulcatus is considered as an important pest of strawberry fields and on potted ornamental plants. The efficacy to control this pest by new Polish products containing azadirachtin and the entomopathogenic fungus Beauveria brongniartii were tested. The aim of the laboratory investigation was to assess the influence of different forms of azadirachtin (A and B) and the entomopathogen on mortality and physiological development of the insect. Mortality after treatment ranged between 86-93%. There were significant differences in the mean number of surviving stages of the insect between Beauveria and neem treatments, but the final total mortality was not statistically different. Sensitivity of all developmental stages to the fungus was observed. In the neem treatments the physiological development of larvae was completely stopped. The level of control achieved by Beauveria and neem for use in outdoor containers is acceptable for practical application.

The evaluation of the effect of the stage of lactation on milk composition, its properties and the quality of rennet curdling was carried out over the period of three successive years using milk samples (n = 162) obtained from a total of 27 ewes of the East Friesian (EF) breed, reared on a small sheep farm in Juřinka in the region of Wallachia. The stage of lactation had a highly significant effect on the contents of all milk components. However, only the contents of total solids (TS), solids non-fat (SNF), fat (F), protein (P) and casein (CN) gradually increased with the advancement of lactation. The stage of lactation also had a highly significant effect both on all the properties of milk and the rennet curdling quality (RCQ). All phenotypic correlations between the particular contents of TS, SNF, F, P, CN and urea nitrogen (UN) were positive and high (P ≤ 0.001). On the other hand, all phenotypic correlations between milk yield and particular contents of TS, SNF, F, P, CN and UN were negative and high (P ≤ 0.001). The majority of phenotypic correlations between rennet clotting time (RCT) and the other particular parameters was insignificant. However, the phenotypic correlations between lactose (L) and RCT and between pH and RCT were positive and high (P ≤ 0.001) whereas the phenotypic correlation between titratable acidity (TA) and RCT was negative and high (P ≤ 0.001). The majority of phenotypic correlations between the rennet curdling quality (RCQ) and the other particular parameters was insignificant. Nevertheless, the phenotypic correlations between pH and RCQ and between RCT and RCQ were positive and high (P ≤ 0.001) whereas the phenotypic correlation between TA and RCQ was negative and high (P ≤ 0.001).

Forty female chickens were allocated to four groups of ten birds each. The control group (K) was fed a basal diet without supplementation. The second control group received a basal diet with humic acid (HA) at a dose of 0.5 g per chicken/day. The first experimental group was fed the basal diet with methyl-mercury (MeHg) at a dose of 0.02 mg per chicken/day and the group of chickens MeHg + HA received 0.5 g HA per chicken/day. After slaughter, mercury levels were assessed in liver, kidney, brain and muscle tissue samples. After 10-day treatment of the chickens with MeHg, significantly increased (P < 0.001) Hg concentrations were detected in all examined tissues in comparison with the groups K and HA. The average concentrations in liver, kidneys, brain and muscle tissues were 424, 398, 81.6 and 79.2 µg Hg/kg, respectively. After concurrent treatment with HA and MeHg, Hg concentrations were lower by 20.6%, 23.8%, 23.0% and 18.6% in liver, kidneys, brain and muscle tissues (P < 0.001). Biological accumulation of Hg was 25.5% and 20.4% in MeHg and MeHg + HA groups, respectively.

The evaluation of nutritional quality of Chinese cabbage and the effects of cultivar, weed incidence, plant density and growing season were observed in integrated cultivation system. Mean contents of analysed compounds were as follows: 6% of dry matter, 10% of crude fibre, (in mg/kg of f.m.): 2,199 mg K, 289 mg Ca, 146 mg Mg, 111 mg Na, and 316 mg of vitamin C. Mean content of nitrates reached the value of 647 mg/kg. A significant effect of cultivar on the content of all observed substances in cabbage heads excluding magnesium was thus confirmed; however, a decrease of nitrates was found in the treatment with higher plant density. Weed cultivation caused slightly (insignificantly) higher nitrates content in cabbage heads by 100 mg/kg. Growing season showed a significant effect on content of some evaluated compounds. Integrated cultivation of Chinese cabbage could be an adequate approach to ensure nutritionally valuable products with low nitrates content.

Important parts of the forest sector are forest engineering constructions and capital construction of forest road network which improve the optimal management of forests and forest stands and their reasonable opening by a forest road network. The basic frameworks of forest road network are forest transport roads of various grades and categories. The paper discusses the basic parameters of forest transport roads such as the length of forest transport roads and their density outspread to the area of a particular district above all. The conditions of forest opening in 41 Natural Forest Regions of the Czech Republic and in the Czech Republic as a whole are shown and confronted on the basis of these parameters. A dilemma of the double division of forest transport roads according to the standard ČSN 73 6108 - Lesní dopravní síť (Forest Transportation Network) and according to the methodology used by Forest Management Institute in Brandýs nad Labem is also described in the paper. Although it is not suitable to use two methods of evaluation, there is no tendency to unify it.

The paper evaluates the development of the tree numbers, secondary stand and species composition on eight partial control plots in Klepačov where the conversion of even aged forest managed under the system involving coupes to selection forest has been implemented within the framework of a long-term project. The plots capture various stages of conversion. Plots A, D, and H still possess structures nearing the forest of age classes. The assessment was made of the measurements taken in 1974, 1994, and 2004. Tree numbers on the partial control plots in the diameter degrees 10-70+ decreased in the first inventory period as compared with both the model situation and the initial situation in 1974 due to increased felling volumes in that period. The felling and the subsequent opening of stands resulted in a rapidly developing regeneration, which manifested itself by the increase in the number of trees in diameter degrees 2 and 6 above the values given by the model curve for these diameter degrees. In the second inventory period, tree numbers in these diameter degrees were lower by 11% as compared with the model situation and slightly lower as compared with the initial situation. Moreover, the results of the survey show that the forest stands under the selection system of management are massively invaded by broadleaved species, namely the beech and that danger exists that broadleaves will dominate in the future. This is generally considered undesirable because the quality of broadleaved stems in the stands under the selection system is low.

The article presents an overview and brief characteristics of the selected soil information systems in the Czech Republic. It suggests synchronisation of their development, particularly some convergence of the Land Evaluation Information System and Soil and Terrain Digital Database. In the pilot area of Litoměřice district, it demonstrates the application of the SOTER methodology for the construction of middle- and detail-scale soil maps, using the data from the General survey of agricultural soils. It not only shows the variety of the district soil conditions, but it also supplements them with the data gathered in the 2006 soil survey.

The common denominator of all rather different names and understandings of it (the problem of the "model of man and society development", problem of the "lack of the civilisation concept" etc.) is that there is no clear understanding regarding the question of the perspective approach (or approaches), the relevant basic development trends of humankind in the material as well as non-material sphere, i.e. in the basic question of many philosophers "Who are we, whence and where are we going". We mention only some of the problems, which are at present directly connected to the "problem of the future of man". These are the problems from the sphere of all-human social, ethical, humanitarian and cultural aspects of the human civilisation development, which are today (but need not be already in the near future) in the centre of attention: the problem of social inequality extension, the problem of spreading epidemics and drug abuse, the problem of the uncontrollable migration and city agglomerations growth, the problem of terrorism.

Migration to herbage of Cyathostominae from experimentally deposited fresh or incubated faecal samples containing a known number of cyathostome L3 was studied in the Czech Republicfor up to 1 year. It was found out that most larvae remained quite close to the faecal samples. Of all larvae recovered from herbage 89.18% were collected within 10 cm of the faeces. Temporal variation in the presence of Cyathostominae larvae on vegeta­tion may account for poor recovery of Cyathostominae L3 in the field. A few infective larvae (0.05?2.74% of the larvae placed on the plot) were found as far as 30 cm from the faeces after 1 or 2 weeks. The number of larvae was significantly higher in June, with maximum recoveries of 4.97% (P < 0.05). Time of day was also significantly related to the number of L3 recovered, larval recovery was greater in the morning than at noontime, the highest number of L3 was observed at 8 am. Moisture and temperature were the most important weather factors associated with lateral larval migrations. There was a closer relation between the larval yields and monthly rainfall (r = 0.47) than between the larval recoveries and weekly rainfall (r = 0.23, r = 0.24). A significant amount of migration occurred during dew. An insignificant amount of migration occurred during dry weather.

This paper presents an overview of methods for detection and identification of the pathogenic bacterium Francisella tularensis such as cultivation tests, enzyme-linked immunosorbent assays, flow cytometry, polymerase chain reaction, immunosensor, microarray, mass spectrometry, and chromatography. Included references are chosen according to their practical importance or perspectives for the future.

A new probiotic, bacteriocin-producing strain of Enterococcus faecium EF9296 (isolate of our laboratory) was used in grass ensiling as an inoculant. The antimicrobial effect as well as the nutritive quality of silage was evaluated along with the aerobic stability test. Grass was ensiled with EF9296 strain (10⁹ colony-forming units per gram of fresh material) and the silage without EF9296 was also prepared. The silages were processed in 1-litre plastic bottles. The samples were collected at days 0-1 (start of experiment), 7, 14 and 105 of ensiling to check the microbial status and chemical parameters of silage. At the end of ensiling period (day 105) the silages were subjected to an aerobic stability test for 7 days. The EF9296 strain survived and colonized the silage in sufficient counts (10⁹-10¹⁰ cfu/g). The total counts of enterococci and lactic acid bacteria were increased. In the silage inoculated with EF9296 strain, a reduction in E. coli, enterobacteriae, staphylococci and Bacillus-like bacteria was noted mainly at day 7 and 14 of ensiling. Moreover, the counts of Listeria and moulds were reduced during the aerobic stability test. The silage inoculation resulted in a more rapid drop of pH, higher level of lactic acid and in a decrease in the concentration of acetic and butyric acid. The effect of E. faecium EF9296 on the value of pH was prolonged until the end of the experiment (including the aerobic stability test, below 4.3). During the whole ensiling period an increase in DM content in the experimental silage was detected. The E. faecium EF9296 strain represents a promising silage additive to produce silage of good quality and/or to prevent silage contamination.