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Inhibition of the in vitro growth of Salmonella enteritidis D by goat and cow milk fermented with probiotic bacteria Bifidobacterium longum Bb-46Vedran Slačanac, Jovica Hardi, Darko Čuržik, Hrvoje Pavlović, Mirela Lučan, Mato VlainićCzech J. Food Sci., 2007, 25(6):351-358 | DOI: 10.17221/752-CJFS This study was carried out to determine the influence of goat and cow milk fermented by Bifidobacterium longum Bb-46 on the pathogenic Salmonella enteritidis D strain. The basic hypothesis of this study was that fermented goat milk could possibly have a stronger inhibitory effect on the growth of Salmonella enteritidis D than fermented cow milk. The correlation between the inhibitory effect and some fermentation parameters (number of viable cells of Bifidobacterium longum Bb-46 and pH of fermented milk) was also analysed. S enteritidis D strains were isolated directly from the faeces of an infant with diagnosed salmonellosis. The inhibitory effects of goat and cow milk fermented with Bifidobacterium longum Bb-46 were determined on Salmonella-Shigella agar after 0, 5, 10, 15, 20, and 25 h from the start of fermentation. Bifidobacterium longum Bb-46 count and pH values were also measured in samples of goat and cow milk during fermentation. The results obtained have shown a considerably higher inhibitory effect of fermented goat milk on the growth of Salmonella enteritidis D as compared to that of fermented cow milk. At the same time, higher acidity and CFU of Bifidobacterium longum Bb-46 were noted in fermented goat milk in all the phases of the fermentation process. The inhibitory effects of the fermented goat and cow milk on Salmonella enteritidis D growth increased rapidly with the fermentation time. The results indicated high sensitivity of Salmonella enteritidis D to acidity of both fermented milks. Consequently, a significant correlation between the inhibition degree and pH values of fermented goat and cow milk was noted. |
Toxicity and spraying schedules of a biopesticide prepared from Piper guineense against two cowpea pestsAlphonsus Mbonu OparaekePlant Protect. Sci., 2007, 43(3):103-108 | DOI: 10.17221/2251-PPS The toxicity of three concentrations (5%, 10% and 20% w/v) and spraying schedules (2, 4 and 6 weekly applications) of an extract from West African black pepper, Piper guineense, for managing two major post-flowering pests of cowpea, Vigna unguiculata, was investigated in two cropping seasons at the Research Farm of the Institute for Agricultural Research, Ahmadu Bello University, Zaria, Nigeria. The insect pests were the larvae of Maruca vitrata (Lepidoptera: Pyralidae) and the cowpea coreid bug, Clavigralla tomentosicollis (Hemiptera: Coreidae). The higher concentrations (10% and 20% w/v) and more frequent applications (4 and 6/week) significantly (P < 0.05) reduced the numbers of the two insect pests compared to the untreated control in both years. Pod damage was significantly reduced and grain yields consequently increased in treated plots compared with the other extract treatments and the untreated control. West African black pepper extract applied at higher concentrations and more frequently could play an important role in integrated management of pests' infestations on field cowpea managed by limited resource farmers in third world countries. |
Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassayV.B. Kandimalla, N. Kandimalla, K. Hruska, M. FranekVet Med - Czech, 2007, 52(10):445-450 | DOI: 10.17221/2045-VETMED During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. Hence, cost effective and simple screening methods are required for residual analysis of environmental and food samples on-site. Here, a simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase tracer conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 µg/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick. |
Influence of Cadophora finlandica and other microbial treatments on cadmium and zinc uptake in willows grown on polluted soilM.N. Dos Santos Utmazian, P. Schweiger, P. Sommer, M. Gorfer, J. Strauss, Wenzel W.W.Plant Soil Environ., 2007, 53(4):158-166 | DOI: 10.17221/2310-PSE We conducted a pot experiment to evaluate the Cd and Zn accumulation in leaves and roots of Salix smithiana (BOKU-03DE-001) and S. caprea (BOKU-01AT-004) clones grown on a metal-contaminated soil as affected by native microbes extracted from the same experimental soil, and the fungus Cadophora finlandica. Plant biomass production of S. smithiana was decreased in all the treatments compared to the sterilized control. In contrast, S. caprea grew best on the non-sterilized soil. Similar effects were observed for plant Zn and Cd contents. Microbial treatments affected metal accumulation differently in the two Salix species. The effects of the microbial treatments on biomass and metal content of leaves were not related to the degree of mycorrhization. A comparison with literature data suggests that the plant response to microbial inoculation in terms of metal accumulation may depend on the plant-internal metal concentration. Our findings also illustrate a difficulty of successful rhizosphere management using metal-tolerant microbial isolates to further enhance the phytoextraction process. |
Freezing point of raw and heat-treated goat milkB. Janštová, M. Dračková, P. Navrátilová, L. Hadra, L. VorlováCzech J. Anim. Sci., 2007, 52(11):394-398 | DOI: 10.17221/2324-CJAS The freezing point (FP) was established in 48 bulk tank samples of raw and 48 samples of pasteurized goat milk that were collected in the course of lactation. Alongside, non-fat solids (NFS) content was monitored. Milk freezing point measurements were carried out using the thermistor cryoscope method in compliance with the standard CTS 570538 (1998). The mean freezing point of raw milk was found to be in an interval of -0.5513 ± 0.0046°C, variation ranged from -0.5466°C to -0.5567°C, with higher values in the spring months and a drop at the end of lactation. FP corresponded to the NFS content. The average freezing point of goat milk heat-treated on the farm to the temperature of 72°C over a period of 20 s was -0.5488 ± 0.0046°C, pasteurisation brought an average increase in FP by 0.0025°C. |
Orchard performance and fruit quality of 50 apple cultivars grown or tested in commercial orchards of the Czech RepublicJ. Blažek, I. HlušičkováHort. Sci. (Prague), 2007, 34(3):96-106 | DOI: 10.17221/1888-HORTSCI Tree vigour, occurrence of mildew and scab, yields, yield efficiency, fruit weight, flesh firmness and soluble solids content of apple trees on M 9 rootstock were monitored between 1998-2005 in 42 commercial orchards situated in all the major growing regions of the Czech Republic. Altogether 50 cultivars were included into the evaluation. The characteristics of newly bred or newly introduced cultivars were compared to the cultivars of standard assortment such as Gloster, Golden Delicious, Idared, Jonagold, Melrose, Rubín and Šampion. The group of late ripening cultivars imported from France (Baujade, Early Smith, Granny Smith and Red Winter) proved to be unsuitable for climatic conditions of the Czech Republic. Cultivars of the Rubín group (Bohemia, Gold Bohemia) showed a better quality of fruits than the cultivars of the Jonagold group, but were significantly inferior regarding yields and yield efficiency. The cultivars Rucla, Pinova and Rubinstep seemed to be potential competitors of Jonagold or Rubín; their fruit quality is similar to Rubín, and their yield efficiency is comparable to that of Jonagold. Topaz, which is resistant to scab and has recently spread in the Czech Republic, is specific for its early yield and a very good productivity observed in a majority of the orchards. In addition, characteristics of other evaluated cultivars and their potential prospects for growing in the Czech Republic are briefly discussed. |
Revised soil erodibility K-factor for soils in the Czech RepublicOriginal PaperJan Vopravil, Miloslav Janeček, Martin TipplSoil & Water Res., 2007, 2(1):1-9 | DOI: 10.17221/2100-SWR In the territory of the Czech Republic there are more than 50% of agricultural soils exposed to water erosion; it is a very urgent problem both at present and for the future. It must be solved now when there is still something to be protected. It is rather complicated to describe the soil properties in terms of soil susceptibility to water erosion because it is a complex relation in which many factors participate. For the complex evaluation of all main factors participating in erosion origination it is possible to apply the Universal Soil Loss Equation (USLE). It consists of six factors interacting with each other and participating in the origination of soil erosion. One of these factors is the soil erodibility factor (K-factor), the revision of which for soil conditions of the CR is the subject of this study. In total ca. 5000 soil pits from the whole territory of the country were processed and evaluated in detail. The main results of this study are K-factor values (means and variances) for the soil types, subtypes and varieties (represented in the database) according to the Taxonomic Classification System of Soils of the Czech Republic. |
Analysis of selected demand factors of wine market of the Czech RepublicT. Pyšný, Z. Pošvár, S. GurskáAgric. Econ. - Czech, 2007, 53(7):304-311 | DOI: 10.17221/1151-AGRICECON This paper analyzes the data on the development of the main factors of the demand for wine and wine consumption in the Czech Republic. The average annual wine consumption, one of the wine demand factors, grew between1991 and 2005 from 14.8 l to 17 l per capita. Following this trend, we can expect the average annual wine consumption 17.4 l per capita in year 2010. However there are critical factors in the development of the demand for wine demand here. Specifically it is the daily feasible ratio of alcohol in wine and other alcoholic drinks, especially beer that we can treat as the substitute of wine. The ethanol consumption in beer accounts for 50% of the total ethanol consumption in alcoholic drinks in the Czech Republic. The negative influence on wine demand growth has been the price of other alcoholic drinks, especially beer that is the cheapest form of ethanol. |
The influence of gypsum treatment on the acquirement of nutrients from soils by barleyJ. Matula, M. PechováPlant Soil Environ., 2007, 53(2):89-96 | DOI: 10.17221/2299-PSE The aim of the present paper was to determine the impact of gypsum treatment of soils on initial growth and nutrient uptake by spring barley. Topsoil from 36 different farmed fields was used for the research. Two variants were established for each soil: control - without gypsum application, and response variant - with the application of 3.3 ppm CaSO4.2 H2O. Barley was grown on these soils for 21 days in a plant growth chamber under controlled conditions of cultivation. Concentrations of nutrients (N, NO3-, P, S, K, Mg, Ca, Na, Fe, Mn, Zn, Cu, B and Mo) were determined in the yield of barley shoot biomass. Paired t-test was used for the evaluation of results. After the gypsum treatment the yield of barley shoot biomass was significantly higher (by 15% on average) and nitrogen utilisation was better on all soils. The concentration of sulphur increased five times on average and Ca concentration increased by 22%. Significant increases were measured in Mg, Mn and Cu. Insignificant differences were recorded in K, Na, Fe and Zn. The uptake of anion nutrients (P, B and Mo) was influenced significantly. The concentration of P and Mo decreased on average by 28% and 31%, respectively. B concentration was higher by 10% on average. |
Performance and carcass quality of Czech Fleckvieh, Charolais and Charolais × Czech Fleckvieh bulls fed diets based on different types of silagesL. Bartoň, V. Kudrna, D. Bureš, R. Zahrádková, V. TeslíkCzech J. Anim. Sci., 2007, 52(9):269-276 | DOI: 10.17221/2267-CJAS A total of thirty-four Czech Fleckvieh (CF), Charolais (CH) and Charolais × Czech Fleckvieh (CH × CF) bulls with an average weight of 284 kg were included in the experiment and fed ad libitum two mixed diets: MS (based on maize silage) and LCS (based on legume-cereal and lucerne silages) with different concentrations of dietary energy until slaughter at the target live weight of 600 kg. The CF bulls consumed more dry matter (DM) of feed daily than the CH bulls (P < 0.05) and gained weight less efficiently than the CH × CF and CH animals (P < 0.01) during the entire experiment. The killing-out percentage was lower in the CF than in the CH × CF and CH (P < 0.05). The CH bulls received a higher score for carcass conformation and a lower score for carcass fatness (P < 0.01) than the CF bulls, had lower proportions of kidney and cod fat (P < 0.01) than the CH × CF and CF bulls, and produced the highest percentage of high-priced meat (P < 0.05) and the lowest percentage of separable fat (P < 0.001). The bulls on the MS diet were younger than the others at the end of the experiment (P < 0.01), gained weight more rapidly (P < 0.001), consumed less DM (P < 0.001) daily, utilized nutrients more efficiently over the entire experimental period (P < 0.001), and had a higher proportion of internal fat than the LCS bulls. It can be concluded that purebred CH bulls were superior to the other breed groups in most of the traits observed. The intensive diet based on maize silage increased average daily gains, reduced the time needed to achieve the target slaughter weight, and improved the feed efficiency of bulls. |
Regime and dynamics of soil moisture in forest ecosystems of Záhorská lowlandL. TužinskýJ. For. Sci., 2006, 52(3):108-117 | DOI: 10.17221/4492-JFS The paper describes the regime and dynamics of the soil moisture content of sandy soils in Záhorská lowland during different growing seasons. Research plots are situated near Kamenný mlyn, approximately 3 km from Plavecký Štvrtok and 8 km southward from the town of Malacky. Changes in the soil moisture content are described by soil moisture constants (MCC, PDA, WP) and its relation to atmospheric precipitation and to the character of undergrowth is shown. The low water-holding capacity of sandy soils and their high drainage together with dense root system do not allow the sufficient saturation of soil during the growing season. The low wilting point value (2%) leads to the consumption of all available water in the soil. The most frequent is the semiarid interval of soil moisture (PDA -WP) with reduced availability of water to plants (> pF 3.1). The arid interval (< WP) occurrence on hot summer days results in a decrease in transpiration and assimilation intensity of plants, their physiological weakening and premature fall of assimilation organs. |
Mineral nutrition in relation to the Norway spruce forest decline in the region Horny Spis (Northern Slovakia)Ľ. Ditmarová, J. Kmeť, M. Ježík, J. VáľkaJ. For. Sci., 2007, 53(3):93-100 | DOI: 10.17221/2148-JFS In this contribution we present the results of analyses of selected mineral nutrients in assimilatory tissues of spruce trees at different developmental phases (plants, adult trees) in the region Horný Spiš. The very close connection between mineral nutrient cycling and other physiological processes in the plants has been well recognised. The presented analyses of mineral nutrient cycling were done within comprehensive eco-physiological research assessing the physiology and health status of spruce stands in the study area. The research was conducted directly in the stand (Hliníky locality, Horný Spiš - two research plots: 1. plot with spruce stand in advanced decline, 2. control plot - without visible decline symptoms) and, at the same time as a pot experiment. The objective of the pot experiment was to verify the supposed negative influence of soil environment (in the locality with advanced decline of spruce stands) on the growth of spruce trees and their mineral nutrient conditions. The analyses of the material sampled from the stands revealed high amounts of manganese (MnT) that were in the toxicity range on both examined plots. Another negative finding was high amounts of toxic aluminium, primarily in needles of adult trees growing on the plot with symptoms of acute stand decline. This reflects a very low value of pH/H2O - 3.7 (pH/KCl - 3 to 2.8) and total exhaustion of the soil suffering, moreover, from the lack of soil water. As for the differences in amounts of individual macronutrients between the plot with intensive decline and the control plot, no significant differences were found, with the exception of Fe. On the other hand, evident significant differences in risk elements Pb, Hg and Al were found. As for the differences in nutrient contents in spruce seedlings in the pot experiment (variants 1 to 6), we can see significant differences in macronutrient contents (N, P, Ca, K, Mn), in some cases also in risk element contents (Al). |
Possibilities of using rooted cuttings of European beech (Fagus sylvatica L.) for stabilisation of forest ecosystemsA. JurásekJ. For. Sci., 2007, 53(11):498-504 | DOI: 10.17221/2021-JFS Vegetative propagation of forest tree species is of great importance not only for breeding programmes but also for the conservation of valuable populations of tree species. Important is also a possibility of improving the genetic quality of established stands. Beech is a tree species that can substantially increase the stability of forest ecosystems. Minimal information is available about the ontogenesis of rooted cuttings of beech in forest stands for the time being. In our experiments that were gradually established since 1993 we acquired the first significant results of the evaluation of health status, phenological phenomena and growth of these plants. Our findings indicate that it is possible to use rooted cuttings as an element stabilising man-made forest stands including the transferred genetic quality. The evaluation of the 2nd generation rooted cuttings of beech also provided positive results. |
Enforcement of the 2003 CAP reform in 5 countries of the West European Union: Consequences on land rent and land marketJ.P. Boinon, J.C. Kroll, D. Lepicier, A. Leseigneur, J.B. ViallonAgric. Econ. - Czech, 2007, 53(4):173-183 | DOI: 10.17221/860-AGRICECON This paper analyses the enforcement of the 2003 CAP reform in 5 countries of the West European Union: France, Germany, Italy, Spain and United Kingdom. The reform gives multiple possibilities of adaptation at a national or regional level. Two standard strategies are foreseen: that of the States which mobilized to the maximum the innovations that the reform allowed, and that of the States which have chosen the option of a minimal application, to limit the effects of reorientation of the productions (maximum sectors remain coupled) or of the redistribution of the payments (historical references). The great diversity of the conditions of agricultural production is one of the main explanations of the differences of enforcement of the reform. We analyse the first impacts of the reform. One can generally expect that the market of entitlements will be a priori limited, because of the links of the entitlements to land. The regionalisation of the calculation of the entitlements is incontestably the mechanism, which introduces the most redistributive effects, compared to the individual historical references. We examine also the consequences of the Single Payment System (SPS) on land rent and land market. |
Determination of volatile organic compounds in the crude and heat treated amaranth samplesM. Ciganek, B. Pisarikova, Z. ZralyVet Med - Czech, 2007, 52(3):111-120 | DOI: 10.17221/1869-VETMED The present study concentrated on the development of an analytical method for determination of emissions of volatile organic compounds from crude and heat treated amaranth (genus Amaranthus L.) samples. Emitted substances were collected by solid-phase microextraction (SPME) method and identified by gas chromatography with mass spectrometry. The list of identified abundant organic compounds exceeds one hundred substances of different classes. Total concentrations of quantified volatile organic compounds ranged between 2.2 and 68.9 μg/g of dried sample. Hexanal and acetic acid were found as the most abundant compounds detected in amaranth samples. It was found that heat treatment (popping) of amaranth samples changed their composition of volatile organic compounds dramatically. The highest volatile organic compound emissions were found in popped grain amaranth in comparison to all crude grains and amaranth biomasses. |
Role of rock phosphate in alleviation of heavy metals stress on Fusarium oxysporumHasan H.A.H.Plant Soil Environ., 2007, 53(1):1-6 | DOI: 10.17221/2288-PSE An endophytic fungus of soyabean (Glycine max) roots, Fusarium oxysporum, was used to study its activity under heavy metals (Cd2+, Mn2+ and Zn2+) stress and the role of rock phosphate (RP) in alleviation of the stress. F. oxysporum growth, amino acids and protein were increased by increasing RP concentration (1-6 g/l) after 8 and 14 days. Heavy metals (HM) have a stressing effect on F. oxysporum - a significant decrease of amino acids, protein and accumulation of sugar at 1mM/l follows a descending order of Cd2+ > Mn2+ > Zn2+. RP is altering the stressing effects of metal on F. oxysporum by a significant growth improvement at 3 and 6 g/l RP, increase of amino acids, protein and decrease of sugar. The solubilization of RP increased under HM application, which may be attributed to an increase of the adsorption of HM by increasing RP concentration. RP has the highest adsorption affinity for Cd2+ (81%) followed by Zn2+ (71%) and Mn2+(55%). |
An influence of cisplatin on the cell culture of Nicotiana tabacum BY-2P. Babula, V. Šupálková, V. Adam, L. Havel, M. Beklová, Z. Sladký, R. KizekPlant Soil Environ., 2007, 53(8):350-354 | DOI: 10.17221/2213-PSE The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied on plant cell lines. When plant cell suspension culture of Nicotiana tabacum BY-2 was treated with cisplatin a decrease of viability with its increasing concentration was observed as well as the mitotic index. The structure of nuclei has also been changed in dependence on cisplatin concentration. |
The effect of animal age on air pollutant concentration in a broiler houseM. Vučemilo, K. Matković, B. Vinković, S. Jakšić, K. Granić, N. MasCzech J. Anim. Sci., 2007, 52(6):170-174 | DOI: 10.17221/2318-CJAS The results of a study assessing the level of airborne contamination in intensive broiler breeding facilities are presented. The content of corpuscular particulates of various origin (dust, bacteria, fungi), ammonia and carbon dioxide was determined. The investigations were conducted in a poultry house on a family farm in the area of moderate continental climate during spring 2006. The air concentration of bacteria ranged from 1.7 × 104 to 2.2 × 105 cfu/m3, of fungi from 9.8 × 103 to 8.5 × 104 cfu/m3, of dust from 1.8 to 4.8 mg/m3, and of ammonia from 4 to 27.47 ppm. Total dust and fungi concentrations measured at the end of fattening period were almost identical to the initial ones, whereas the concentrations of bacteria and ammonia showed a sinusoidal rise from the beginning to the end of fattening period. In general, the analyzed air pollutants reached relatively high levels in the mid-fattening period and also show significant differentiation between fattening periods as demonstrated by t-test yielding statistical significance at a level of P < 0.05. |
Dynamic laboratory measurement with dielectric sensor for forage mass flow determinationF. Kumhála, Z. Kvíz, J. Kmoch, V. ProšekRes. Agr. Eng., 2007, 53(4):149-154 | DOI: 10.17221/1959-RAE A new parallel plate capacitance sensor was built consisting of two metal sheets. The sensor - a capacitor and the whole oscillating circuit was driven at 27 MHz frequency. Dynamic laboratory experiments were performed with grass from a natural meadow in order to evaluate the possibility of the forage mass flow determination by means of this sensor. The results revealed a relatively strong linear relationship between the feed rates of the wet forage crop material passing through the sensor between its plates and the measured capacitance sensor circuit output frequency. The coefficients of determination (R2) varied from 0.9 to 0.96. Further improvement of the electronic circuit connection and further investigation of the sensor can be recommended. |
Development assistanceV. Jeníček, V. KreplAgric. Econ. - Czech, 2006, 52(5):209-224 | DOI: 10.17221/5018-AGRICECON Development assistance achieved remarkable success in different periods. For example, Botswana and South Korea reached the great development in the 60s after very bad situation, Indonesia in the 70s, Bolivia and Ghana at the end of the 80s, Uganda and Vietnam in the 90s. In these countries development assistance played important role in economic transformation in formulation of the development of politics. The development assistance contributed educational programs and financially supported the development of public sector. The "Green Revolution" - by means of innovations in agriculture, investments and political changes - improved the live conditions of millions people thanks to the collaboration of many bilateral and multilateral donors. But there are some failures with the foreign aide. While the formed dictator of Zaire Mobutu Sese Seko became one of the richest people in the world (and invested his property in abroad), the development assistance did not stop for many years, Zaire (now the Democratic Republic of Congo) is only one example of the situation, where the permanent flows of assistance ignore or support the corruption and in suitable politics of governments. Tanzania received two milliards dollars for building the roads destiny the twenty years. But the roads were destroyed sooner, than the works could be finished because of insufficient maintenance. The study of World Bank brings the conclusions of the new conception of the development assistance: financial assistance works only in suitable political world; the lowering of poverty is possible only with working institutions - political and economic; effective assistance complete the private investments; receiving country is obliged to have public sector in function; the function of public sector is developing on the activity of civil society; patience and good ideas, not only money, can help to reforms in very unfavorable conditions. |
International brand strategy of Taiwanese hi-tech enterprisesJ. Tsai, H.-Y. HungAgric. Econ. - Czech, 2007, 53(2):83-88 | DOI: 10.17221/903-AGRICECON This paper compares two Taiwanese high-tech enterprises, Acer and Trend Micro, which choose different international brand marketing strategies due to the diverse managing types and product characteristics. The hardware products and business software sold by Acer and Trend Micro are discussed to reveal their international brand development and marketing strategy. The research findings can be provided for different enterprises for decision-making when they consider their internationalization and brand strategy. |
Limiting factors in the field of business activities in rural tourismK. RyglováAgric. Econ. - Czech, 2007, 53(9):421-431 | DOI: 10.17221/628-AGRICECON This paper deals with problems of rural tourism development. The works is focused on researching the situation in the area of rural tourism in the Czech Republic, on understanding the attitudes of entrepreneurial sphere and rural population to this entrepreneurship and mainly on the determination of limiting factors that prevent this form of tourism from a more distinctive development. Rural tourism in the Czech Republic is still in the initiating stage of its development and it is not as developed as in some countries in West Europe. This goal has been reached with the help of primary questionnaire inquiry among business and agricultural subjects in rural areas in the individual regions and the detail results of this questionnaire inquiry are enclosed to the following paper. These identified factors were mainly insufficient financial means and at the same time insufficient state assistance connected with this. Insufficient awareness of this support as well as its inaccessibility for small business appears to be a difficulty, too. From the other obstacles, we can mention the current legislation system, bureaucracy, the state of communication and tourist infrastructure, fears of neighbours' intolerance and losing privacy and also unfamiliarity and little experience. |
European brown hare as a potential source of zoonotic agentsF. Treml, J. Pikula, H. Bandouchova, J. HorakovaVet Med - Czech, 2007, 52(10):451-456 | DOI: 10.17221/2050-VETMED There has recently been a growing interest in checking the state of health of European brown hares in hunting grounds because they are a susceptible bio-indicator of environmental changes and because of the dramatic decline in populations across Europe. A total of 1 051 (384, 302, and 365, respectively) blood sera were collected from hares during autumn hunting events and examined for tularaemia, brucellosis and leptospirosis in 2004, 2005 and 2006. Antibodies were found in 6.5, 1.6 and 7.5% of cases, respectively. Antibodies against Francisella tularensis, Brucella suis and leptospires were found in 7.9, 1.7 and 7.7% of females and 4.5, 1.4 and 7.9% of males, respectively. Higher seroprevalence of tularaemia was found in females (P = 0.05). Brucellosis was more prevalent in adult animals compared to subadult ones (P = 0.05). Only the L. grippotyphosa serotype was found and the titres mostly ranged from 100 to 400 (89.9%). Higher titres from 800 to 3 200 were found in the remaining 10.1% of the examined European brown hare sera. Antibodies against F. tularensis as well as B. suis were most frequent in low titres of 10 to 80. Higher titres were only exceptional. Confirming that hares are susceptible to various zoonotic agents, it is necessary to be careful when handling the animals killed. On the other hand, blood sera from hares may be used to survey the occurrence of natural nidi of zoonoses in hunting grounds. |
Root excretion and plant tolerance to cadmium toxicity - a reviewJ. Dong, W.H. Mao, G.P. Zhang, F.B. WU, Y. CaiPlant Soil Environ., 2007, 53(5):193-200 | DOI: 10.17221/2205-PSE Significant quantities of Cd have been added to soils globally due to various anthropogenic activities, posing a serious threat to safe food production and human health. Rhizosphere, as an important interface of soil and plant, plays a significant role in the agro-environmental system. This article presents a review of relationship between root excretion and microorganisms and plant resistance to Cd toxicity and possible mechanisms. Root exudates markedly altered in species and quantity under Cd stress. Root exudates can affect Cd absorption by plants through changing the physical and chemical characteristics of rhizospheres. The influence of root exudates on Cd bioavailability and toxicity may include modifying the rhizosphere pH and Eh, chelating/complexing and depositing with Cd ions, and altering the community construction, the numbers and activities of rhizospheric microbes. In this paper, the methods to reduce the transfer of Cd in soil-plant system by adjusting rhizosphere environment are discussed, and some aspects are also proposed that should be emphasized in the future research work. |
The effects of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoramAli R.M., Abbas H.M., Kamal R.K.Plant Soil Environ., 2007, 53(12):529-543 | DOI: 10.17221/2188-PSE he study was undertaken to determine a possible role of polyamines (putrescine, spermidine, spermine) as antioxidants in salt tolerance of Chamomilla recutita and Origanum majorana. Salinity generally induced variable changes in growth, contents of oil and flavonoids of both plants; foliar application of any polyamines counterbalanced the effects of salinity. In general, the degree of stimulation differed according to the type and concentration of the used additive and the type of the plant. |
Functional state of mammary gland of sows dried off by different techniquesA. Rekiel, K. Czumińska, J. Więcek, W. Bielecki, J. KulisiewiczCzech J. Anim. Sci., 2007, 52(3):62-69 | DOI: 10.17221/2358-CJAS Sows were dried off by a traditional method (control - C, restricted feeding before, during and after weaning) or by a modified one (experimental - E, intensive feeding before, during and after weaning). On day 5-6 after weaning of the 3 |
Supplemental wheat bran and microbial phytase could replace inorganic phosphorus in laying hen dietsJ.H. Yao, J.C. Han, S.Y. WU, M. XU, L.L. Zhong, Y.R. Liu, Y.J. WangCzech J. Anim. Sci., 2007, 52(11):407-413 | DOI: 10.17221/2321-CJAS An experiment was conducted to determine effects of wheat bran (WB) phytase on production performance and nutrient utilization in laying hens. Three hundred and seventy-five Lohmann hens at 32 weeks of age were randomly allotted to treatments of fifteen hens per pen with five pens per treatment. Five experimental diets were formulated. Diet one (control) contained 0.19% inorganic phosphate (Pi) from dicalcium phosphate. On the basis of diet 1, diet 2 and 3 were formulated to contain WB 5% and 10%, respectively. In diet 4 and 5, the WB was fixed at the level of 10% with Pi level adjusted to 0.14% in diet 4 and completely replaced with 500 U/kg microbial phytase in diet 5. The results showed that treatment three improved egg yield (P = 0.142), feed conversion ratio (FCR) (P = 0.011), utilization of crude protein (CP) (P = 0.060) and total phosphorus (tP) (P < 0.001), and serum Pi concentration (P = 0.016) compared with the control. Ten percent of WB replacing 0.05% Pi did not influence either egg yield or nutrient utilization. Compared with the control, treatment five improved FCR (P = 0.011) and utilization of CP (P = 0.060) and tP (P < 0.001), but did not influence either performance or serum parameters. The current study suggests that wheat bran could be used successfully in laying hen diets and wheat bran and microbial phytase supplemented together could replace inorganic phosphate completely. |
In vitro selection of NaHCO3 tolerant cultivars of Morus alba (Local and Sujanpuri) in response to morphological and biochemical parametersP. Ahmad, S. Sharma, P. S. SrivastavaHort. Sci. (Prague), 2007, 34(3):114-122 | DOI: 10.17221/1889-HORTSCI In vitro experiments were conducted to study the effect of NaHCO3 (alkalinity) stress on saplings of Morus alba (cv. Local and Sujanpuri) cultured from nodal explants. For shoot multiplication 2.5 mg/l of 6-benzylaminopurine (BAP) with 0.3 mg/l gibberellic acid (GA3) were used and root formation was induced with 1.0 mg/l of indolebutyric acid (IBA). NaHCO3 salt was added to the culture medium in three concentrations, i.e. 3.57, 20.0 and 59.0mM that increased pH to 6.2, 7.2 and 8.2, respectively. The increased salt concentration affected survival and growth parameters, subsequent cultures promoted them. The cultured biomass was analyzed for proline, protein, sugars and chlorophyll content. The results indicate an increase of proline, protein and sugars; however, they declined at higher concentrations of NaHCO3. A decrease of chlorophyll was observed at all stress regimes. |
Processes of loss, recruitment, and increment in stands of a primeval character in selected areas of the Pieniny National Park (southern Poland)A. Jaworski, R. PodlaskiJ. For. Sci., 2007, 53(6):278-289 | DOI: 10.17221/2017-JFS Studies were carried out during 1987-1997 in four stands situated in the lower mountain zone, and representing the association Carici-Fagetum abietetosum (sample plots Facimiech and Walusiówka) and the community of a transitory character between Dentario glandulosae-Fagetum and Carici-Fagetum (sample plots Gródek and Przelecz Sosnów). The greatest volume increment was found in a pure fir (Abies alba) stand of Facimiech (9.4 m3/ha/year, i.e. 1.4% of actual stand volume determined in 1997) being in the optimum stage, phase of aging and regeneration, and the smallest one stand of Gródek (5.3 m3/ha/year, i.e. 0.9% of actual stand volume) being in the growing up stage, phase of selection forest. The stand of Przelcz Sosnów was characterized by the greatest mortality of trees (volume of loss, i.e. 13.5 m3/ha/year). In this stand of a transitory character between the growing up and optimum stages, due to rapid mortality of fir the break up of the stand took place, and in consequence the growing up stage, phase of a little diversified stratified structure was developed. Volume of recruitment was the greatest in the stand of Facimiech, i.e. 0.05 m3/ha/year. In three fir (Abies alba)-beech (Fagus sylvatica) stands the proportions of fir and beech in stand increment differed from their proportions in stand volume. The percentage of fir in volume increment was smaller, and that of beech greater, than their percentages in stand volume. Processes of increment and mortality of fir and beech pointed to a progressive process of changes taking place in stand species composition, expressed by the increase of beech and the decrease of fir. The knowledge about values of loss, recruitment, and increment, expressed by the number of trees and volume units, may be of help in determination of the amount of cut in productive and protective forests managed according to a close-to-nature silviculture. |
