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The subject of this paper was to assess the effects of pulverised dolomitic limestone applications on prosperity of Norway spruce (Picea abies L.) plantation and on the soil in extreme ecological conditions of the Jizerské hory Mts. The characteristics such as plant mortality, annual height increment, diameter of root collar (stem base diameter), crown diameter, nutrient analyses and some data from soil analyses are evaluated in this paper. An eleven-year period of observations is summarised. According to the observations and measurements carried out until now, the effect of limestone applications (1 kg per tree) on the spruce plantation prosperity seems to be positive without any serious negative impacts on the soil.

Previous experiences with breeding of lime tree (Tilia cordata Miller) in European countries are shown in this paper. The first results were obtained from the evaluation of juvenile planting of lime tree (Tilia cordata Miller) in the Czech Republic, founded in the School Forest Enterprise Kostelec nad Černými lesy. Lime tree clones (initially cultivated in vitro) were evaluated for growth parameters and also according to the growth form of the different clones. Individual clones were also checked for mortality.

Various attacks of pathogenic fungi on the larch crown were observed in Trentino/South Tyrol, Italy, over the past few years. In 1999, severe defoliation was caused by Mycosphaerella laricina, a rarely investigated pathogen, whose action was enhanced by high temperatures and humidity in August. In July 2000, larch woods showed crown yellowing due to Meria laricis. Lower temperatures and heavy precipitation were probably the predisposing factors in this case. Hypodermella laricis was observed in 2002 as a localised attack, while Lachnellula wilkommii is commonly recorded. The impact of these diseases and the environmental factors involved in their occurrence are discussed in this paper.

The article summarises the results of the analysis of value added (VA) in agriculture and food industry and the share of intermediate consumption in the value added. The results show that during 1993-2000, the Slovak agricultural sector (farming, hunting, forestry and fisheries), where farming is clearly dominant, together with the construction sector, reported the most significant decline in their relative contribution to the overall value added created in the national economy, whereas the contribution of market services increased. The moderate increase (1998, 1999) in the contribution of the food sector to the total value added in the national economy, as well as to industrial production (2000), has been brought to a halt and, eventually, began deflating. The tendency of declining participation of the agricultural sector in value added was also typical for the EU member states and for the CEFTA countries. Agriculture remains dominant in terms of its contribution to value added in the agri-food sector (54.5% in the year 2000). In the category of land-farming holdings, the share of intermediate consumption in value added increased (both in co-operatives and business companies, whether loss-making or profitable). The farmers in mountainous areas spent per 1 SKK of value added 0.65-1.42 SKK more of intermediate consumption than farmers in the maize production area and 0.54-1.32 SKK more than farmers in the sugar-beet production area. In the production of foodstuffs, beverages and tobacco processing, which is more demanding in terms of consumed inputs than agricultural production (the same is true for developed economies), the ratio between intermediate consumption and value added differed depending on the branch concerned. Agriculture was one of the most demanding sectors in terms of fixed asset investments necessary to generate value added. The initial decline was brought to stop in 1998 and the relative share of fixed assets (FA) in value added (VA) started to increase, particularly in the year 2000, mainly under the influence of increased support to farmers' capital investments. Although the creation of value added in agricultural co-operatives initially demanded more investments in fixed assets than it was the case in farming business companies, the difference narrowed over the time. The group of loss-making entities reported the FA/VA ratios twice as high as the group of profitable entities, which sends a signal to the former to revise the structure of their production and to improve management practices. Similarly as in the case of intermediate consumption, also the FA/VA ratio increased as the production conditions deteriorated. The ratios in the production of foodstuffs and beverages (without tobacco processing) were significantly lower than those in the farming sector. In 1999, the lowest FA/VA ratio occurred in the production of durable bakery products and the highest in the production of fruit and vegetable juices.

The objective of the article is to describe the possibilities of the rural development in the Bratislava region. The rural region is represented by 71 municipalities in districts Malacky, Pezinok, Senec and by parts of the city Bratislava - Záhorská Bystrica, Vajnory, Jarovce, Rusovce and Čunovo. The analyses of the rural region are the segments of the Single Programming Document (SPD) for the 2004-2006. It aims to support the areas with the main economic and social problems according the Goal 2. The article deals with the development possibilities in industry, market services, tourism and agriculture. The branch structure, main economic parameters of effectiveness and production, employment, wages are analysed in period 1999-2002. The article contains also the results of SWOT analysis and the short-term activities to supporting rural development.

The paper analyses formation of specialized activities of Slovak agrarian services. The paper focuses attention on the definition of agrarian services, evaluation of their position and progress of their transformation, their quantitative development and distribution in the regions of the Slovak Republic, as well as on their economic results during the period from 1995 to 2001. Biological, technical, and other specialized services significantly influence the reproduction process of the plant and animal production even in the period of European integration.

The presence and distribution of PPV-M strain of Plum pox virus were investigated in selected orchards of apricots and peaches in the Czech Republic. PPV-M was found to be distributed in apricot and peach orchards planted with nursery material imported from abroad. The presence of PPV-M was not proved in orchards planted with trees from local nurseries. The absence of PPV-M in a majority of spontaneous PPV hosts was proved previously. PPV-M strain appears to have been introduced recently.

Power saws require a relatively large amount of oil for their operation the reason being idle lubrication of the cutting chain. Oil leakages in the environment can result in soil and water contamination and are known to impact both flora and fauna. Many countries in Europe struggle to minimize the damage by imposing the use of biologically degradable oils for these purposes. The work presents an assessment of these biologically degradable oils from the viewpoint of their possible occurrence in environment and from the viewpoint of their technical characteristics which affect reliability and economy of working with power saws. Oil dispersion is evaluated especially from the viewpoint of the concentration of its occurrence on the soil surface after cutting with the power saw. Technical properties evaluated include heat-oxidation stability and pumping properties of oils at low temperatures. There are original methods of measurement developed for these purposes, which facilitate high repeatability of results. The effort aims at oils with core technical parameters at least comparable with mineral oils used so far, which would represent a lesser burden on natural environment.

This article presents information about the development and the structure of indices regarding natural and legal persons in the years 2000 and 2002. Finally, the development of indices is compared with the year 1990. The article characterizes the development of companies, which are farming land, like cooperatives, business companies, state owned companies and natural persons. It demonstrates the number and structure of companies in the branches of the agri-food sector divided into agriculture, agricultural services and food industry. Moreover, the article presents agricultural land area used by natural and legal persons. This contribution draws information from the results of the questionnaire, which was made by the regional offices of the Ministry of Agriculture. In conclusion, the article suggests the possible orientation of organizations in the short time period

The paper is a part of solution of the grant awarded by the Ministry of Agriculture (NAZV) No. QF 3276 and analyzes the influence of the price level of imported bottled wine on the competition in the wine-production sector in the Czech Republic. The comparison of the industrial producers' prices in the Czech Republic with the average import prices of bottled wine in particular years brings us to conclusion that a threat for Czech producers is first the price of the imported table wine (white and red) at present. The average import prices of this wine category varied under the minimal average industrial producers' prices in 1998-2003. The average import price of the white table wine in containers up to 2 liters was 19 CZK per liter in 2003 and the minimal average industrial producers' price was 26.90 CZK per liter in the same year. The price level is higher in case of the red table wine in general, but the average annual import prices (in 2003, 23 CZK per liter) also varied under the minimal average industrial producers' prices in all analyzed years (in 2003, 29.70 CZK per liter). The situation is more positive for the Czech wine producers in case of the quality wine. There is a space for an increase in price. The average import prices were by 25 CZK per liter per year higher in average than the maximal industrial producers' prices in the Czech Republic in all analyzed years. However, while the average annual import price of the white quality wine increases (50 CZK per liter in 1998; 93 CZK per liter in 2003) and creates a bigger space for the Czech wine producers in the price policy, the average annual import prices of the red quality wine varied around 80 CZK per liter in all analyzed years.

This article sets out to analyze the value added generation in the Slovak agrarian sector at the time of the accession to the EU compared to which we are severely lagging behind in the overall generation of gross value added. A more detailed analysis of the value added generation over the recent years in a group of agricultural enterprises being legal entities indicates that the enterprise efficiency expressed in terms of value added generation is markedly differentiated depending on the natural conditions of the locations where individual businesses operate. Great differences in the added value between enterprises can also be found in the group of enterprises having the same natural conditions. The reason for this is a differentiation in the degree to which intensification inputs are utilized due to high prices, lack of financial resources, inefficient combination of inputs used and insufficient recognition of the importance of the value added by managers, particularly in planning of production structures and production finalization grade.

The requirements for compliance with the manufacturing and quality standards and the related investments in the hygienic and structural upgrading of establishments before Slovakia's accession to the European Union meant that the Slovak food processors had to intensify their effort, revise their objectives (where necessary) and increase the volume of investments, particularly during 2003. Many entrepreneurs, particularly in the animal production, decided to go out of business, mainly because the continued operation of obsolete slaughterhouses would require considerable investment effort; at the end of the day, some meat producers closed their slaughter operations and focused on specialised meat processing. Compared with the year before, the profit of food processors declined by 23%. This was due to the faster decline in revenues than expenses, increased cost of revenues, and the reduced output. Although profitable companies still prevailed, the percentage of loss-making companies increased in comparison with the previous year. The starch industry, sugar industry and the confectionery (including coffee substitutes) industry were some of the sectors where all operators were profitable.

A possibility to increase the efficiency of plant regeneration from microspore-derived embryos of selected botanical varieties of Brassica oleracea was investigated from 2001 to 2004. More than 400 regenerants of R₁ generation were derived in kohlrabi, cabbage and cauliflower by means of different modifications of microspore culture technique. Distinct genotype differences in embryogenic responsibility and regenerative ability of microspore embryos to whole plants were detected. The highest frequency of embryogenesis and subsequent regeneration of plants were achieved in cauliflower cultivar Siria F1, kohlrabi line P7 and some experimental F1 hybrids of cauliflower. The best production of embryos was obtained when donor plants were grown in the growth chamber under controlled light and temperature conditions. The regeneration of plantlets was considerably improved by repeated subculture of cotyledonary embryos on media with various combinations of phytohormones and excision of the cotyledons from mature embryos. The percentage of plant regeneration from subcultured embryos in kohlrabi ranged from 11.11 to 63.64%, in cauliflower from 23.53 to 46.19% and in cabbage from 5.88 to 52.00%. The utilization of regenerants for doubled haploid line production is often complicated by male sterility also in plants with the normal diploid chromosome number.

The object of this study was to investigate the effect of pruning on heartwood formation in mature Scots pine (Pinus sylvestris L.) trees. Fifty trees were treated by three different intensive pruning regimes: 42, 60 and 70 percentage of defoliation. After five growing seasons numbers of growth rings were counted and the width and the area of sapwood and heartwood were calculated. The results did not show any proportional increase or decrease in the heartwood area or in the number of growth rings in heartwood associated with the pruning. A statistically significant negative effect of pruning was found on the width of the five most recently formed sapwood growth rings. This decreased growth rate did not influence the ratio of sapwood and heartwood. However, it cannot be excluded that the proportion of heartwood may increase during a longer period. It is concluded that pruning is not a practicable silvicultural method for regulating heartwood formation in mature Scots pine trees.

Dothistroma needle blight caused by Mycosphaerella pini E. Rostrup was observed for the first time in the Czech Republic on an imported Pinus nigra Arnold in 1999. In 2000, it was also found in the open planting. During three years, it became an important pathogen of pines in the Czech Republic. Its occurrence was noticed in more than 50 localities, above all in the region of Moravia and Silesia and eastern Bohemia. In total, it was found on 10 species of pine (P. nigra Arnold, P. banksiana Lamb., P. contorta Loudon, P. mugo Turra, P. leucodermis Ant., P. sylvestris L., P. cembra L., P. aristata Engelm., P. ponderosa P. et C. Lawson and P. jeffreyi Grev. et Balf.). Also Picea pungens Engelm. was noticed as a host species. In the Czech Republic, Pinus nigra is the most frequent host species of M. pini (80% localities) followed by Pinus mugo (27% localities). On Scots pine P. sylvestris, M. pini was noticed at two localities. The critical period for infection is in the Czech Republic from the second half of May until the end of June. The incubation period lasts about 2-4 months depending on climatic conditions. The first symptoms on the needles infected in the current year appear in August being clearly expressed from September to November.In the CR, Dothistroma needle blight spread probably with infected planting stock obtained from import at the end of the 80s and at the beginning of the 90s.

The paper focuses on identification of the current key movement forces in wine-production industry in the Czech Republic, and its goal is to analyse these key influences, and to identify the most important ones (long term industry growth-rate, technological changes, regulation influences and changes of government policy, and changes of societal priorities and life style). Based on the identified key factors influencing future development and changes in this industry, recommendations for firms are formulated.

A study of diprionids associated with spruce was done at 31 sites of the eastern part of the Czech Republic in 1988-2003. In total 45 specimens belonging to Gilpinia abieticola (6 specimens), G. hercyniae (3) and G. polytoma (36) were collected using Malaise traps, emergency traps, bark-beetle pheromone baited traps or sweeping. The spruce diprionids were recorded in 30% of the studied sites only. G. polytoma was the most abundant and it is probably bivoltine with adult activity from April to August. The results of emergence trap-monitoring in Paskovský les indicate that this species is in latency.

Except of 2001 and 2002, the agriculture has produced losses of SKK 38.8 billion over the entire process of transformation. The losses in agriculture were caused by major disparities between the price of supplies to agriculture and prices of agricultural products, plus the restrictive subsidy and loan policy adopted in the early years of the economic reform. The economic situation has improved over the last two years. This was caused by the increase in subsidies, as well as by the continuing restructuring process (sales and liquidation of dubious assets, optimised production), reduction in numbers of loss-making enterprises, by growth and increase in efficiency of production and due to a substantial increase in earnings of many partnerships. However, even despite the positive trends current financial position of most agricultural enterprises does not meet the requirements for development in agriculture, with the rate of TFA (tangible fixed assets) depreciation achieving 50% (of that, depreciation of machinery stands at 70%). The earnings are moderate even in profit making enterprises, with 75% of those enterprises making only up to SKK 1.5 million in earnings.

The paper includes partial results of the research oriented on the habits and behaviour of consumers in the wine market in the Czech Republic. Based on direct questioning of 1 000 respondents from the whole Czech Republic, we found that 11.3% of respondents drink wine several times a week, 29.8% of respondents at least once a week, and 23.2% drink wine several times a month. Only 2.7% of respondents do not drink wine at all. 30.3% of respondents drink wine more often than before, especially because of the fact, that they like wine more, it is of a better quality, and the offer is bigger. 34.1% of respondents buy wine several times a month especially in supermarkets or hyper- markets (45.2%) and in special vine-stores (23.8%). 52.5% of respondents prefer quality varietal wine. Rank of the top ten wines most often bought by the selected sample of respondents is: Frankovka, Cabernet Sauvignon, Müller Thurgau, Veltlínské zelené, Rulandské bílé, Svatovavřinecké, Modrý Portugal, Chardonnay, Ryzlink vlašský, Bohemia sekt.

Competitiveness can be analysed at various levels of the economy: at the product level, the enterprise level, the sector level, or the level of the entire economy. Several measures exist for each of these levels. This paper focuses on those used for the analysis of enterprises, particularly agricultural enterprises farming in Slovakia. Our research has been done on a selected sample of agricultural enterprises farming in Slovakia. Using "Recourse Cost Ratio" (RCR coefficient) has allowed investigating the differences in competitiveness between co-operatives and business companies, between the enterprises farming in better and worse soil and natural conditions as well as among various size groups of 111 agricultural enterprises. The research has proved that the optimal values of RCR coefficient revealed, that the common feature of all the competitive enterprises was their type of production oriented on solely plant production, possibly plant and meat production (there was no case of competitive enterprise with solely meat production). As for the different legal forms, we found out that more competitive were business companies than co-operatives. Considering different soil and natural conditions, there were better results observed in the group of enterprises farming in better soil and natural conditions.

N fertilization has a substantial influence on sunflower (Helianthus annuus L.) seed yield and quality. It was also well established that high-yielding sunflower hybrids had more N requirement than old cultivars such as open-pollinated ones. However, in Turkey, no sufficient information regarding the response of new developed oilseed sunflower hybrids to nitrogen fertilization under irrigated conditions. Therefore, a 2-year study was conducted to determine the effects of nitrogen application rates on the growth, yield, and yield components of two oilseed sunflower hybrids (AS-508 and Super 25) under irrigated conditions. In this study, all plant parameters were significantly influenced by applied nitrogen fertilizer rates. Yield response to nitrogen rates was positive and linear. Our research data indicated that under irrigated conditions N rate of 120 kg/ha was adequate for sunflower production in this region.

The fulfilment of the subject of the Act No. 76/2002 Dig. on integrated prevention reduces energy consumption and increases hygienic safety of a thermal treatment of animal tissues at veterinary decontamination institutes. By making use of a back supply of heated rendering-plant fat to the continuous disc drier KDS 250 the so-called fritting effect is induced speeding up the evaporation of residual water and reducing the redrying time of the meat and bone paste. An analysis proved a reduced consumption of specific energy, whereby the demand for the best available technique (BAT) is met.

A total of 15 strains of Psychrobacter immobilis isolated from animal sources, e.g. cheese, fish and poultry, were tested. A commercial diagnostic kit NEFERMtest 24, conventional tests and determination of fatty acids were used for identification. By using the results of NEFERMtest 24 and numerical identification system TNW version 6.0 the identification was successful on the species level (46.7%) while the correct species identification by using conventional tests increased up to 86.7%. All 9 saccharolytic strains including 7 Czech isolates were identified in most cases on an excellent or very good level by both methods based on biochemical reactions. On the other hand, the identification of 6 asaccharolytic strains was unsuccessful especially by NEFERMtest 24. While 4 asaccharolytic strains were identified correctly on the basis of conventional tests on species or genus level, incorrect identification on species level, for example Ralstonia paucula, Comamonas terrigena, Oligella ureolytica, Moraxella lincolnii andPsychrobacter phenylpyruvicus, was found by using NEFERMtest 24. Determination of fatty acid composition by MIDI System confirmed the species identification of 9 out of the 10 tested strains of P. immobilis and 1 tested strain of Psychrobacter sp.

In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector. Recombinant protein was expressed in E. coli cells and soluble fraction of the protein was subsequently purified by Ni2+-chelate affinity chromatography. Purified protein was then used as antigen in an indirect ELISA test. One hundred fifty ovine serum samples were screened for antibodies to p25 protein of the virus. Immunoblot with whole virus antigen was used as a gold standard. The total number of positive results in the ELISA was 38 (25.33%). Immunoblot failed to confirm a positive result in 2 (1.33%) of them and these results were therefore considered to be false positive. The number of true positive results in the ELISA was thus 36 (24%). All immunoblot positive samples were also positive by ELISA test. In conclusion, recombinant His-tagged capsid protein showed very high sensitivity and specificity in detecting antibodies to Maedi-Visna virus.

A daily dose of 14 mg atrazine and 2.5 mg zearalenone, given p.o. during 5 days of estrous cycle to female rats, changed their estrous cycle in comparison with control animals. On day -1 of expected estrus, a significantly lower (p < 0.05) concentration of 17b-estradiol compared with the control group was recorded in all experimental groups of animals. In the group of animals administered zearalenone, the concentration of 17b-estradiol on the day of expected estrus was significantly higher (p < 0.05). In the group administered a combination of atrazine and zearalenone, the concentration of 17b-estradiol on the day after expected estrus was significantly higher (p < 0.05) than in the control group. In the group of animals receiving atrazine, complete absence of the onset of estrous cycle was recorded, whereas in the group given zearalenone the onset of estrous cycle was delayed by 24 hours. The combination of atrazine and zearalenone induced similar effects as atrazine, however, with the onset of estrous cycle being delayed by 48 hours. Neither of these two groups of animals reached the level of 17b-estradiol recorded in the control group.

The paper widely introduces European experience in wild cherry breeding because it is the first one from a prepared series of articles aimed at wild cherry breeding in the Czech Republic. Beginnings of wild cherry breeding program for the Czech forestry are described. Plus trees were certified, seed orchards, clone archives, progeny and clonal tests were established. Clones were tested for fl owering of grafts in reproductive plantations. Progenies and clones were tested for growth parameters in progeny and clonal tests. Progenies and clones in every breeding plantation were tested for damage by aphids.

The basic principle of the economic reform of state forests of the CzechRepublic after 1990 was to separate ma­nagement in forests from the implementation of production activities. A state enterprise Forests of the Czech Republic with its headquarters in Hradec Králové was charged to manage state forests. From the former 7 enterprises of state forests 78 joint-stock companies have been established that carry out silvicultural and logging activities in state forests and in forests of other owners under the conditions of competition environment and on the basis of contracts. The paper deals with the exact evaluation of partial and present results of the development of forestry economic reform, aimed at the study of the behaviour of transformed and privatised forest joint-stock companies.

Yellowing of Norway spruce is a relatively common problem in many regions of the Czech Republic. At some places, it is also connected with forest decline. This unfavourable phenomenon is initiated by several ecological as well as anthropogenic processes. One of the most important is the imbalance of forest stand nutrition. Particular reasons lead to nutrient deficiency, especially deficiency of magnesium. Solution to this undesirable trend is a profound ecological analysis and cause-oriented treatments. Besides decreasing air pollution impacts, fertilisation with deficient nutrients represents a successful treatment in the forest stand management in affected areas. In the area of interest, application of relatively small amounts of appropriate fertiliser (SILVAMIX Mg) led to considerable improvement in the defoliation dynamics and yellowing progress since the first year after application.