Fulltext search in archive
Results 4501 to 4530 of 4599:
Analysis of the selected problems of agricultural business entrepreneurship in SlovakiaP. Bielik, M. BeňoAgric. Econ. - Czech, 2002, 48(5):225-228 | DOI: 10.17221/5307-AGRICECON Even ten years after the revolution in 1989, Slovak agriculture is characterized by the predominant position of cooperatives. There were shifts towards other types of agricultural entrepreneurship, and some of these trends we documented in our article, based on the data gained by surveying agricultural enterprises. We drew a conclusion that cooperatives are more labor demanding. This also means that they still preserve a social role as a main employer in rural areas. Our paper also differentiates between Southern and Northern (less favorable for agriculture) regions, represented by the Nitra and Žilina region, respectively. We examined soil quality, labor intensity, wages, and finally main crops yields in these two regions. |
Analysis of the yield milk effect on the economics of milk productionP. KopečekAgric. Econ. - Czech, 2002, 48(10):473-479 | DOI: 10.17221/5355-AGRICECON Relations between the level of milk yield and the economic results of dairy cows breeding were analysed on the base of operational and economic data received from 135 agricultural enterprises. The analysis was aiming at the optimization of milk production expressed by means of the cost function. By evaluating the above-mentioned relations, there was recorded a tendency of the faster growth of milk yield compared to the costs for market milk for one feeding day of a dairy cow. On the base of the expense function, there was expressed the maximum profit for a litre of market milk, the maximum profit for a dairy cow per year and the interval of profitability of milk production in 2000. |
Secondary succession on an early abandoned field: vegetation composition and production of biomassJindra ŠtolcováPlant Protect. Sci., 2002, 38(4):149-154 | DOI: 10.17221/4871-PPS During 1996-2000 the secondary succession on a field left fallow was investigated. The experimental area was divided into a ploughed and an unploughed part. Both parts were divided into 5 × 5 m plots that were either left intact (control), mowed in June or July, or superficially cultivated in June. The position of the plots was identical each year. In successive years total dry matter production decreased in all experimental plots; in unploughed plots more than in ploughed ones. The spread of Cirsium arvense (L.) Scop. was increased by ploughing and superficial cultivation. Ploughing stimulated the spread of perennial dicotyledonous plants more than mowing in June and July. Superficial cultivation increased the spread of perennial dicotyledonous plants in the first years. Later on the proportion of perennial dicotyledonous plants decreased and after 5 years the plots were dominated by Anisantha sterilis (L.) Nevski. The incidence of Elytrigia repens (L.) Desv. was highest in the 5th year on unploughed control plots. The spread of perennial monocotyledonous plants was lowest in ploughed and superficially cultivated plots. |
Genotypic characterisation of the Erwinia genus by PCR-RFLP analysis of rpoS geneM. Waleron, K. Waleron, E. ŁojkowskaPlant Protect. Sci., 2002, 38(11):288-290 | DOI: 10.17221/10470-PPS Genotypic characterisation of the members of the genus Erwinia, based on the PCR-RFLP analysis of a fragment of the rpoS gene was done. PCR primers deduced from described rpoS gene sequences of E. carotovora allowed the amplification of about 880 bp DNA fragments from all tested Erwinia species. The rpoS fragments, amplified from 20 species of the studied Erwinia genus, were compared by RFLP analysis with 4 enzymes (AluI, Hin6I, HinfI, and Tru1I). Restriction analysis allowed drawing 63 common profiles of RFLP products for all tested Erwinia. From 1 to 3 specific RFLP profiles were identified among most of the species tested. However, in two cases: E. chrysanthemi and E. c. subsp. carotovora 15 and 20 specific RFLP groups were detected, respectively. High variability of genetic profiles of the E. chrysanthemi and E. c. subsp. carotovora can be explained by the wide spectrum of plants, which they infect. The results indicated that rpoS PCR-RFLP analysis is a useful tool for identification of species and subspecies belonging to the former Erwinia genus, as well as for differentiation of strains within E. c. subsp. carotovora and E. chrysanthemi. |
Albugo candida (white rust) suppresses resistance to downy mildew pathogens in Arabidopsis thalianaCooper A.J., A. Woods-Tör, E.B. HolubPlant Protect. Sci., 2002, 38(11):474-476 | DOI: 10.17221/10527-PPS Arabidopsis thaliana accessions were inoculated with incompatible isolates of downy mildews, following pre-inoculation with compatible Albugo candida. Three isolates of Hyaloperonospora parasitica subsp. A. thaliana, an isolate of H. parasitica subsp. Brassica oleracea and one Bremia lactucae (lettuce) isolate were included. All downy mildews sporulated on A. thaliana, suggesting A. candida suppresses broad-spectrum downy mildew resistance. The white rust resistance gene, RAC5, is being investigated. The resistance phenotype associated with RAC5 seems not to involve a hypersensitive response. RAC5 has been mapped telomeric of nga106 on chromosome 5, in a region lacking NB-LRR genes, the most common structural class of resistance genes known in A. thaliana. |
Effects of temperature on in vitro response of Trichoderma strains against strawberry pathogen Rhizoctonia solani Kühn.M. Porras, C. Barrau, B. Santos, F.T. Arroyo, C. Blanco, F. RomeroPlant Protect. Sci., 2002, 38(11):620-622 | DOI: 10.17221/10572-PPS Effect of temperature on growth and antagonistic ability of Trichoderma spp. isolated from local strawberry culture and commercial product, against Rhizoctonia solani, strawberry pathogen, was studied in vitro. Trials were carried out twice, at 10, 25 and 30°C. Inhibitor effect was evaluated by radial growth measures of established duals on PDA's dishes, using Royse and Ries formula, to evaluate the percentage inhibition of radial growth. Design of dishes was a randomized complete block, considering 10 replicates. Data were analyzed statistically by two-way analysis of variance. The objective has been to determine the most competitive Trichoderma strain and the best temperature that produce the inhibiting effect on the pathogen growth. Local strain has the best behavior at 10 and 25°C. |
Changes in requirements on vernalization of winter wheat varieties in the Czech Republic in 1950-2000J. Petr, F. HniličkaPlant Soil Environ., 2002, 48(4):148-153 | DOI: 10.17221/4213-PSE The need for vernalization of winter wheat varieties cultivated in the CzechRepublic in 2000 was studied in comparison with the need for vernalization in the past decades since 1950. In 2000, many foreign varieties were cultivated in the Czech Republic, mostly West European. Varieties with a vernalization of 40-50 days and 50-60 days show the highest representation in the assortment (47.3% and 31.6%, resp.). The share of varieties with a long vernalization over 60 days is 15.8%. In around 1990, when varieties of domestic breeding were mostly grown, there were, next to the largest group with a vernalization of 40-50 days, 21.7% of varieties with a vernalization of 30-40 days and the same amount with a vernalization of 50-60 days. During the last ten years, the share of varieties with a longer vernalization has risen, not only due to foreign varieties, but also due to new domestic varieties. It is apparent from a 50-year overview that what has predominated are varieties with a vernalization of 40-50 or 40-60 days, which is a range usual for winter varieties of wheat in Middle and West Europe. After 1950, a departure from original domestic varieties appeared; those were represented by original alternative varieties (in Czech přesívky, in German Wechselweizen, in Russian dvuručki) and half-winter varieties with a shorter vernalization, strictly speaking with a vernalization fixed to a short day, and a strong photoperiodic reaction. Representation of varieties as related to their length of vernalization has changed in the course of the decades following utilization of foreign varieties; this was affected above all by varieties from Russia (the former USSR), Germany, but also Yugoslavia. Varieties from these countries were utilized also as parent components in domestic breeding. |
Barley seed sensitivity to water stress at germination stageV. Hosnedl, H. HonsováPlant Soil Environ., 2002, 48(7):293-297 | DOI: 10.17221/4370-PSE Barley seed sensitivity to water and anoxia was tested. Standard germination, mean time of germination (MTG), germination in sand wetted by water to 100% water capacity (anoxia) or by hydrogen peroxide (wet conditions without anoxia), germination in 0.75% hydrogen peroxide and laboratory emergence (15 and 20°C) were evaluated. Barley seed responds sensitively to stress conditions during germination. Significant germination decrease was found in abundance of water. Percentage of reduction depends on the variety and on the year of seed production. Extreme values of water sensitivity are in interval 4-90%. At wetted sand by 0.75%, solution of H2O2 the germination was significantly less reduced. That means that barley seed is very sensitive to oxygen deficiency above all and is less injured by quick imbibition. Heterogeneity in seed vigour was demonstrated in laboratory emergence tests. Quick test of germination in 0.75% hydrogen peroxide deserves attention for its high correlation coefficient with the seed laboratory emergence. The results significantly demonstrate a higher sensitivity of deteriorated seed to germination in abiotic stresses conditions. Variability in speed of germination is increasing, which unfavourably extends the mean time of germination. |
Rapid hydroponic screening for molybdenum tolerance in rice through morphological and biochemical analysisG.R. Rout, P. DasPlant Soil Environ., 2002, 48(11):505-512 | DOI: 10.17221/4404-PSE High yielding varieties of rice (Oryza sativa) cultivars were tested for their tolerance to different levels of molybdenum (Mo) (0.1µM - control, 0.2, 0.4, 0.8 and 1.6µM) in nutrient solution at pH 6.8. Seeds of rice were germinated and grown in presence of molybdenum under controlled environmental conditions. Standard growth parameters such as root length, shoot length, root/shoot dry biomass and root/shoot tolerance index were tested as markers of molybdenum toxicity. Measurements as early as 48 hours after the germination did not yield consistent results. However, root measurement on 3rd, 6th and 9th day after root emergence showed significant differences among cultivars of rice. Rice cultivars Annapurna, Kusuma, Deepa and Vaghari developed better root system while, Paridhan-1, Pusa-2-21 and Ratna showed poor growth of the roots in presence (0.8µM) of molybdenum. The root tolerance index (RTI) and the shoot tolerance index (STI) in Annapurna, Kusuma and Deepa in rice were high indicating their tolerance to molybdenum; Paridhan-1 and Ratna, however, showed low RTI and STI. Based on the growth parameters, twenty cultivars of rice were ranked in respect of their tolerance to molybdenum: Annapurrna > Deepa > Kusuma > Vaghari > Hamsa > Vikram > Bharati > Paridhan-2 > Aswathi > Subhadra > Sankar > Sakti > Nilgiri > Rudra > Hema > Pragati > Pusa-2-21 > Ratna > Paridhan-1, respectively. Molybdenum toxicity was correlated with increased peroxidase and catalase activity in different cultivars of rice. This method can be employed for quick screening of rice cultivars for molybdenum tolerance in breeding programmes. |
Winter wheat RheiaL. Bobková, V. Šíp, M. ŠkorpíkCzech J. Genet. Plant Breed., 2002, 38(2):90-91 | DOI: 10.17221/6119-CJGPB |
Fruit weight, firmness and soluble solids content during ripening of Karešova cv. sweet cherryJ. Blažková, I. Hlušičková, J. BlažekHort. Sci. (Prague), 2002, 29(3):92-98 | DOI: 10.17221/4470-HORTSCI Changes in fruit weight, firmness and soluble solids content during the ripening period of Karešova cv. Including relationships between these characteristics were studied in 1998-2001. These years differed from each other in the amount and distribution of rainfall and in the course of temperatures. The length of ripening period fluctuated from 14 to 21 days. An increase in average fruit weight during the ripening period was 0.1 g per day. The actual increase in fruit weight was in a close correlation with the amount of rainfall in a few previous days. After heavy rainfalls, above 40 mm, fruit weight increased by nearly 1 g within in a few days. In the course of the ripening period fruit firmness decreased from about 2.5 N at the beginning of the period to about 1.5 N at its end. This decrease seems to be mainly the function of time during ripening. However, a low correlation between the amount of rainfall in the previous days and the decrease in firmness was also found. This correlation could be connected with negative regression of fruit firmness on the weight of the fruit. In the more rainy years 1998 and 1999 these decreases in firmness were more significant than in the other two years. Soluble solids content (SSC) increased from about 12% Brix at the beginning of the period to about 16% at its end. There were also found positive correlations between the weight of fruits and SSC. Taking into account contributions of all studied factors and their changes a conclusion was drawn that Karešova cv. should be harvested within one week from the beginning of its ripening period. |
Age and diameter classes or growth stages as criteria for the implementation of thinningitle not given--Original PaperR. PetrášJ. For. Sci., 2002, 48(1):8-15 | DOI: 10.17221/11852-JFS Age and diameter classes or growth stages as criteria for the implementation of thinning |
Chrysopids and Hemerobiids (Plannipenia) of young spruce forests in the eastern part of the Czech RepublicOriginal PaperJ. Holuša, Ľ. VidličkaJ. For. Sci., 2002, 48(10):432-440 | DOI: 10.17221/11912-JFS Chrysopids and Hemerobiids were studied using Malaise traps in young spruce forests in the eastern part of the Czech Republic. A total of 12 species were found. The most abundant species were eurytopic Chrysopa carnea, Chrysopa perla, Hemerobius humulinus and Hemerobius pini associated with conifers. The seasonal flight activity of these species is discussed. |
Ethnocentrism and consumer evaluations of Czech made yoghurtU.R. Orth, Z. FirbasováAgric. Econ. - Czech, 2002, 48(4):175-182 | DOI: 10.17221/5300-AGRICECON In identifying perceptions and attitudes relevant to consumer acceptance of goods originating in different countries, it would be extremely helpful for marketers to have a meaningful and consistent measure that they could apply. This study examines to what extent consumer ethnocentrism as measured by the CETSCALE singularly, as well as in concert with selected demographic and psycho-graphic variables, can predict consumers' evaluation of domestic versus foreign produce. The findings indicate that consumer ethnocentrism is a strong and significant predictor of consumer product evaluations. Including the ethnocentrism variable in a set of demographic and psycho-graphic variables significantly improves the predictive ability of the set. Potential applications of the concept include identification of market segments that react more favourably to domestic or foreign produce, developing effective marketing communication strategies, and supporting location decisions for retail outlet sites. |
The evaluation of economic situation and comparison of Czech and French agricultural enterprisesF. Simon, J. NovákAgric. Econ. - Czech, 2002, 48(9):389-394 | DOI: 10.17221/5342-AGRICECON The comparison of Czech and French agriculture results shows a higher intensity, productivity and profitability of French agriculture. There are just small differences in the effectiveness of the production. The Czech agricultural enterprises have been in economic distress for several years and only a low portion of them is able to modernize and increase reproduction. There is also highlighted the importance of the EU Common Agricultural Policy for the development and stabilization of French agriculture. |
Authenticity of 100% orange juice in the Czech market in 1996-2001M. Voldřich, P. Skálová, F. Kvasnička, P. Cuhra, M. Kubík, P. PyšCzech J. Food Sci., 2002, 20(2):83-88 | DOI: 10.17221/3514-CJFS Commercial orange juices samples obtained from the Czech market were analysed in the years 1996-2001. The quality and authenticity of samples was evaluated according to the Code of Practice of AIJN (selected main analytical criteria were followed - K+, Na+, Ca2+, Mg2+, citric, isocitric and malic acid, citric acid/isocitric acid ratio, glucose, fructose, saccharose, sorbitol, formol number, flavonoid glycosides - according to Davis and HPLC procedure, refractive index and other). The approach of producers to the quality and authenticity of juices developed during the years of observation. The main cases of adulteration in 1996 were as follows: (i) lower fruit content in juice, (ii) massive addition of sugars masked with addition of citric acid, (iii) several examples of "synthetic" orange juice were found. In the subsequent years the authenticity slightly improved, the main problems in 2000/2001 were: (i) lower refractive index, (ii) pulp wash addition, (iii) lower quality of water used for juice reconstitution, (iv) undeclared addition of sugar. The deviations were found not only in the case of the juices of Czech producers, but also in several discount and low-end products of foreign producers. Possible ways of improving the quality and authenticity are discussed (e.g. the preparation of a Czech National Standard taking over the requirements for juices and nectars according to the Code of Practice of the AIJN, Participation in the European Quality Control System [EQCS], etc.). |
Antibiotic production of the biocontrol agents Epicoccum nigrum and Candida sakeOriginal PaperI. Larena, M. LIÑÁN, P. MelgarejoPlant Protect. Sci., 2002, 38(10):205-208 | DOI: 10.17221/10356-PPS In the framework of the study of the mode of action of biocontrol agents (BCAs) it is important to know if BCAs areantibiotic-producers. Epicoccum nigrum 282 and Candida sake CPA-1 are BCAs effective against post-harvest pathogensof stone and pome fruits. The antibiotics produced by these BCAs and the relationship to biocontrol were studied.Production of antibiotics by E. nigrum in in vitro cultures began at 5 days of incubation being maximal at different timesdepending on media used. However, no antibiotic was detected when E. nigrum was grown in a solid state-fermentationsystem or in peaches. In the case of C. sake, no antibiotic was detected either in vitro, in liquid fermentation culturesof the yeast, or in apples. |
Comparison of two wheat powdery mildew differential sets in seedling testsG. Vida, L. Szunics, M. Gál, O. Veisz, Z. BedőPlant Protect. Sci., 2002, 38(11):417-420 | DOI: 10.17221/10510-PPS Two wheat powdery mildew differential sets were tested in the seedling stage in the 2001/2002 season using 192 monoisolates. The data of genotypes carrying the same Pm gene in different genetic backgrounds were compared. Both varieties with gene Pm8 (Salzmünde14/44 and Disponent) were infected by all the isolates. Less than 10% of the isolates gave different responses on varieties with genes Pm2 and Pm3c (6 and 16, respectively). It is doubtful whether the degree of infection of genotypes carrying genes Pm1, Pm4b, Pm5, Pm6 or Pm7 can be compared, while it is completely impossible to compare the data for varieties from the old and new sets carrying genes Pm3a, Pm3b and Pm4a. |
Occurrence and intensity of more important fungal diseases on soybean (Glycine max (L.) Merrill) cultivarsT. Duvnjak, M. Vrataric, A. Sudaric, J. CosicPlant Protect. Sci., 2002, 38(11):590-592 | DOI: 10.17221/10563-PPS The main objective of this study was to determinate the occurrence and intensity of attack by the more important fungal diseases on soybean in Eastern Croatia. Four-year investigation (1998-2001) was undertaken on 10 domestic soybean cultivars with different level of disease resistance. The investigation was set in two planting date (optimal and delayed) on experimental fields of the Agricultural Institute Osijek. Following more important diseases were established: Downy mildew, Pod and stem blight and Stem canker. Significant differences in disease incidence were caused by genetic diversity of tested materials as well as different climatic conditions among growing seasons. According to planting date, significantly higher incidence of Downy mildew and Pod and stem blight was in optimal planting date. |
The effect of fluridone and flurochloridone on the incidence of albinism in pea (Pisum sativum) and on the abscission of leaves of privet (Ligustrum vulgare)Š. Klíčová, J. Šebánek, M. Hudeová, H. Vítková, H. VlašínováPlant Soil Environ., 2002, 48(6):255-260 | DOI: 10.17221/4237-PSE The effect of fluridone (or flurochloridone), the inhibitor of carotenoid, chlorophyll and abscisic acid synthesis, on the abscission of Ligustrum vulgare leaves was investigated. Both forms of fluridone inhibited the abscission of petioles when they were applied as a 1.0% concentration in lanolin to the leaf blade. Fluridone was capable of inhibiting abscission even when it was applied to the petiole as late as 7 hours after the blade was cut off. Fluridone applied in lanolin to the apical part of intact pea seedlings or on the cut surface after decapitation of the epicotyl caused albinism of the stipules. The degree of albinism decreased according to the concentration of applied flurodine (from the highest - 0.5% to the lowest - 0.03%) and was higher in intact than in decapitated plants. Albinism also appeared in pea seedlings grown from seeds swollen in differently concentrated solutions of flurochloridone, particularly in the basal part of the shoots. Complete albinism occurred only in plants cultivated from seeds swollen in high concentrations (0.06-0.12%). The lowest concentration (0.007%) stimulated root growth of the pea seedlings, but inhibited epicotyl growth. |
Wine volatiles composition in the sensory evaluation of bouquet and flavour of two vine cultivarsJ. Balík, J. Goliáš, J. Veverka, M. Kyseláková, A. Němcová, L. Šuderlová, J. VejrostaHort. Sci. (Prague), 2002, 29(1):4-11 | DOI: 10.17221/4463-HORTSCI Analytic measurement data acquired by an SFE/CGC analysis were compared with sensory evaluation of two wines from South Moravia. The sensory descriptive analysis showed a large variation in sensory quality from neutral character to full expression of the variety. Whereas the cluster analysis of volatiles was sufficient for differentiating both wines, the sensory observation data based on seven descriptors did not suffice either for bouquet or flavour characteristic. The descriptor citrus could be related to analytically established substances whose statistical significance was supported by a stepwise multiple analysis. The possibility of recognising vine cultivars via sensory descriptors is statistically feasible only by nutty and caramel descriptors in the case of bouquet and by big fruit for flavour. |
Globalisation and regionalisationV. JeníčekAgric. Econ. - Czech, 2002, 48(2):87-92 | DOI: 10.17221/5293-AGRICECON What regards the vision of the future relationship of the global and regional liberalisation, two variants are offered. According to the first, multilateralism will go on perpetually around the present trajectory and gradually will, supported by the processes of internationalisation and interdependence, accelerated by the condensing net of trans-national corporations and their activities, suppress regionalism. According to the second, regionalism will, closely connected to multilateralism, spread territorially into the shape of several macro-regions as a transitive stage to the unified liberalised world economy. In both cases, it regards of course the visions of a system and not matter-of-fact type. In the frame of each region, there will further exist different comparative advantages, which will influence the volumes and structure of production and trade, as well as certain specific fields of economic policy. However, that changes nothing of the fact, that namely multilateralism contributes to a considerable extent to reaching a higher equilibrium, adaptability and coherence of the world economy as a whole, even if reaching of this state is connected with considerable, mostly, however, short-time, costs. The contribution characterises globalisation and regionalisation: its contents, types and dimensions, TNC, unequality of the globalised economic development. |
The adjustment of agrarian business structure to the European agrarian structures during pre access period into the EUV. GozoraAgric. Econ. - Czech, 2002, 48(8):333-338 | DOI: 10.17221/5330-AGRICECON The article deals with the preparation of the agricultural business structure for accession into the European agrarian structures in the pre-accession period. Main attention is devoted to the preparation of agrarian management and to changes in the macroeconomic area. From the viewpoint of the information mentioned above, the paper includes the explanation of the agrarian functions in the agrarian sector and prediction of the development of business base in the sectors of agriculture, food processing industry and services. The synthetic part deals with the integration and globalization tendencies in the agricultural and food production. |
The development potential of Czech rural areas and rural tourismT. HájekAgric. Econ. - Czech, 2002, 48(12):559-562 | DOI: 10.17221/5368-AGRICECON In spite of the official goals of support for rural tourism promulgated by governmental bodies, rural tourism remains a marginal phenomenon. This lack of confidence in rural tourism has relatively deep roots. The basis for this is almost of an ontological nature and consists in opposition, although subconscious, against "commodified authenticity". Another source of this lack of confidence lies in the fact that rurality, the main attractive feature of tourism, has been substantially impaired as a result of trends in the 20th century. A third reason follows from the combination of rural tourism and mass recreation, as well as inadequate response by governmental bodies and authorities to the risks for regional stability, based on unmanaged mass tourism. |
The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivarsJiban Kumar KunduPlant Protect. Sci., 2002, 38(1):13-17 | DOI: 10.17221/4816-PPS The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard. These two main pome fruit viruses were detected frequently in all tested apple cultivars. ASGV and ASPV occurred in as many as 16 trees (in the cultivar Spartan) and 13 trees (in the cultivar Idared) out of 20 tested trees, respectively. Mixed infection by ASGV and ASPV was found in all tested cultivars (as many as 9 out of 20 tested trees of the cultivar Spartan). |
Sugar beet as a raw material for bioethanol productionA. Hinková, Z. BubníkCzech J. Food Sci., 2001, 19(6):224-234 | DOI: 10.17221/6612-CJFS Overproduction of sugar causes a reduction in the acreage under sugar beet. That is why new non-food technologies for exploitation of agricultural products are sought. Utilization of beet for liquid fuel production could be one of them. The aim of experiments with sugar beet raw juice fermentation was to verify the possibility to return a part of distiller's slops back to the fermentation process and thereby to obtain stillage with higher content of dry solids. This would bring about energy savings during slops thickening and drying. Tests with recycling of different portions of stillage (20, 25 and 30%) back to the fermentation stage were carried out. No significant increase in dry solids content in mash was found and therefore no energy savings during thickening can be expected. The only savings can be made in water consumption that is replaced by slops. |
Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)Original PaperD. Vašková, J. ŠpakPlant Protect. Sci., 2002, 38(10):S24-S27 | DOI: 10.17221/10313-PPS Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBV-NASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR. |
Population dynamics of the leafhopper Psammotettix alienus Dahlb. and two-year investigations into the occurrence of Wheat dwarf virus (WDV) in crops of winter barley located in the Middle German Dry Region, GermanyS. Mehner, B. Manurung, D. Schmidt, M. Grüntzig, W. Witsack, E. FuchsPlant Protect. Sci., 2002, 38(11):370-374 | DOI: 10.17221/10494-PPS From 2000 to 2001 the population dynamics of Psammotettix alienus Dahlb. were recorded using a sweep-net or a biocoenometer. The investigations were carried out in Zscherben near Halle (Middle German Dry Region). The imagines of the first generation of P. alienus could be observed for the first time at the beginning of May (2000) and at the end of May (2001), respectively. According to our results, in this area three generations of P. alienus are developed. In both years of our observations the barley-strain of Wheat dwarf virus (WDV) occurred. The main important infections appeared in autumn. Furthermore, during the whole period of our investigations the percentage of viruliferous individuals which were caught was recorded by means of a biological test. In June this percentage achieved 84.0% (2000) and 76.7% (2001), respectively. In the course of summer months of both years the percentage of viruliferous P. alienus decreased. In autumn of the year 2000 an increasing portion followed once again. However, in the year 2001 a continual reduction from > 70% in June to < 5% in late autumn could be observed. |
Effect of the product of radiational removal of SO2 and NOx from combustion gases on the population of Globodera rostochiensis nematodes (Woll., Behrens 1975)G. Kaup, K. Janowicz, K. ParuchPlant Protect. Sci., 2002, 38(11):642-644 | DOI: 10.17221/10579-PPS The studies were carried out in a greenhouse of the Agricultural University of Szczecin, during two growing seasons. The aim of this study was determine the effect of the product obtained from radiational SO2 and NOx removal from combustion gases on the populations of Globodera rostochiensis nematodes - one of the most common quarantine organisms. The analysis of the effect of the applied product on the population of nematodes demonstrated their varied character of response. In the combination with the product of radiational purification of combustion gases, also significantly lower population density (number of eggs and larvae in 1 g of soil) and lower fecundity of Globodera rostochiensis females were observed compared to the control combination. And in the second year of the experiment, the number of cysts in the combination with the product of SO2 and NOx removal from combustion gases was significantly lower compared to the control, and was less than half of the quantity of the previous year. |
