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Results 481 to 510 of 4598:

Impact of cover crops in inter-rows of hop gardens on reducing soil loss due to water erosionOriginal Paper

David Kabelka, David Kincl, Jan Vopravil, Petr Vráblík

Plant Soil Environ., 2021, 67(4):230-235 | DOI: 10.17221/24/2021-PSE

Soil degradation caused by water erosion in sloping hop gardens is definitely a serious issue because the space in inter-rows is without plant residues for most of the year in traditional cultivation. Cover crops in inter-rows of hop gardens and their efficiency in soil conservation are assessed in this article. There is only little research available in this area, and our data bring unique information on water erosion in hop gardens. Technologies with different types of cover crops were always compared with the conventional cultivation. The research was conducted within the years 2016-2020. A field rainfall simulator was used to determine the soil conservation effectiveness of selected technologies. The simulated rainfall was performed in two stages of cover crops growth with the main aim to measure the overall soil loss. The outcomes from the measurements confirmed that cover crops in inter-rows of hop gardens protect the soil surface from falling raindrops and significantly (P-value < 0.05) reduce soil loss. It can be concluded that this technology had a soil conservation effect already one month after sowing, and it is a basis for sustainable agricultural management on sloping hop gardens.

Efficacy of a therapeutic bath with selected antiparasitic drugs on a Dactylogyrus anchoratus infection in juvenile common carp (Cyprinus carpio)Original Paper

J Kolarova, E Zuskova, J Velisek

Vet Med - Czech, 2022, 67(12):620-627 | DOI: 10.17221/66/2022-VETMED

The study aimed to assess the effects of a therapeutic bath of five different antiparasitic drugs, in different baths and durations: fenbendazole (25 mg l-1, 12 h and 2 × 12 h), formaldehyde (0.17 ml l-1, 15 min), ivermectin (0.031 mg l-1, 1 h), mebendazole (1 mg l-1, 12 h) and levamisole (50 mg l-1, 2 h and 3 × 1 h) on the reduction on the intensity and prevalence of a monogenean infection (Dactylogyrus anchoratus) in juvenile carp. The best effect on reducing the parasite number was achieved with the bath in formaldehyde (0.17 ml l-1, 15 min) and fenbendazole (25 mg l-1, 2 × 12 h with 24 h break), where the infection was reduced by more than 90%. Registered veterinary medicinal products (VMPs) with the active substance of fenbendazole can successfully replace the use of unregistered formaldehyde in the treatment of monogenean infections.

Effect of plant essential oils on the mortality of Ditylenchus dipsaci (Kühn, 1857) nematode under in vitro conditionsOriginal Paper

Ondøej Douda, Miloslav Zouhar, Marie Maòasová

Plant Soil Environ., 2022, 68(9):410-414 | DOI: 10.17221/92/2022-PSE

With the need to obtain new methods to protect seed material from Ditylenchus dipsaci (Kühn, 1857) nematodes, a study was conducted to evaluate the nematicidal effects of several plants' essential oils on the mortality of D. dipsaci. Tests were performed under in vitro conditions; a concentration of 2 000 ppm was tested, nematodes were added into diluted oils, and numbers of living/dead nematodes were scored after 4 and 24 h. The results show a significant effect of several plant essential oils on D. dipsaci mortality, with the highest efficacy found for oil from Cinnamomum cassia ((L.) J. Presl), with 100% mortality observed even after 4 h.

Obituary - Prof. MVDr. Karel Hru¹ka, CSc.Biographical Notice

E Baranyiova

Vet Med - Czech, 2022, 67(7):331-333 | DOI: 10.17221/35/2022-VETMED

With the passing of Professor MVDr. Karel Hru¹ka, CSc., on March 24, 2022, the Czech veterinary community has lost an outstanding figure in basic and applied veterinary research. His remarkable legacy also includes bringing the discipline into the computer era as well as enduring contributions as an educator and editor.

Spruce forest litter structure, distribution, and water retention along hiking trails in the Ukrainian CarpathiansOriginal Paper

Yurii Ivanenko, Ganna Lobchenko, Volodymyr Maliuha, Vasyl Yukhnovskyi

J. For. Sci., 2022, 68(7):241-252 | DOI: 10.17221/12/2022-JFS

Almost 24% of the Ukrainian Carpathian ecosystems are assigned to the conservation fund due to their ecological values and attraction to numerous tourists. The forest litter in mountain forest stands plays an important role in terms of its ability to mitigate the impact of tourist activities, and erosion processes along with its contribution to the soil mineralization. Water interception, infiltration, and retention ability of forest litter have an impact on hydrological processes of forest ecosystems. At the same time, the accumulation and spatial distribution of litter can be affected not only by environmental conditions, but also by tourism. In this study, 13 Norway spruce (Picea abies L.) stands distinguished by average distance to the trail were chosen to investigate whether there are any differences in litter structure, water retention, and infiltration abilities as well as litter accumulation in areas along popular hiking trails in the Ukrainian Carpathians. Results showed that the litter thickness has increased with altitude and slope steepness. Moreover, results of one-way ANOVA demonstrate a significant difference (P < 0.05) in litter stock between two groups of sample plots: established directly to adjoin hiking trails and at a distance. Therefore, the forest litter stock nearby hiking trails may indicate that trampling caused by tourists has a negative impact on litter accumulation. However, no statistical difference was found (P > 0.05) in the accumulation of litter along three trails and altitudes across all surveyed forest stands. Results of the immersion test showed that the litter infiltration rate has a significant negative correlation with the maximum mass of absorbed water (r = -0.62, P < 0.05), litter stock (r = -0.69, P < 0.01), and retained precipitation (r = -0.62, P < 0.05). Despite our assumption, the infiltration rate was lower for sites distanced from the hiking trail in comparison with adjoining ones. According to our measurements, the water holding capacity of the litter varies from 42.3 t.ha-1 to 187.3 t.ha-1 regardless of the stand composition. Further, the precipitation amount retained by litter varies between 4.2 mm and 18.7 mm. Insignificant differences in litter fractional structure and accumulation were observed in pure spruce stands and mixed spruce-beech with an admixture of beech up to 20%. Further observational and modelling studies are necessary to clarify the role of the beech share in stand composition in relation to fractional structure and water interception and retention ability.

Seasonal abundance of insects from a vineyard in southern RomaniaShort Communication

Diana Elena Vizitiu, Ionela-Daniela Sãrdãrescu, Andrei Tiþa, Elena-Cocuþa Buciumeanu

Hort. Sci. (Prague), 2022, 49(1):52-58 | DOI: 10.17221/15/2021-HORTSCI

A field experiment was undertaken in a vineyard in southern Romania to evaluate the insect species abundance in this temperate climate area, over a period of 24 consecutive weeks, starting from April 2nd to September 27th, 2019. A total number of 19 303 insects were collected. After their morphological analysis, 89 species of insects were identified (nine orders and 28 families), of which 52 came from beneficial fauna (six orders and 14 families, 1 316 individuals) and 37 came from pest fauna (six orders and 26 families, 17 987 individuals). The beneficial species richness of the Coleoptera and Hymenoptera orders were the best represented. The pest insects of the Coleoptera and Hemiptera orders registered a high number of families, while the Thripidae family had the highest number of individuals. The highest diversity of insect species was found in the middle of April, and the lowest was found in the middle of August. The Bray-Curtis analysis revealed the stability of the insect community in the studied grapevine growing area. Grouping insects based on their similarities revealed the highest similarity (91.05-97%) in the warmest periods (June-August) and the lowest similarity (33%) in the spring (April).

Measurement of post-dispersal invertebrate seed predation of some relevant weed species in maize fields in Hungary: An ecosystem service provided in crop fields contributing to weed managementOriginal Paper

Mohammed Gaafer Abdelgfar Osman, Mark Szalai, Mihaly Zalai, Zita Dorner, Jozsef Kiss

Plant Protect. Sci., 2022, 58(4):351-359 | DOI: 10.17221/159/2021-PPS

Invertebrate seed predation is a potential ecosystem service that substantially reduces weed seeds in crop fields, decreasing the seedling emergence and, thus, limiting the weed competition next season. It may, thus, be considered as a natural component of the long-term weed management toolbox. This study aimed to measure the post-dispersal invertebrate seed predation levels of the following relevant weed species in Hungarian maize fields: Ambrosia artemisiifolia, Datura stramonium, Chenopodium album, and Echinochloa crus-galli, and to compare the predation levels among them. We hypothesised that invertebrate seed predators will predate weed seeds, but the predation levels may vary with the weed species. Two sampling rounds were performed, in November 2019 and October 2020, in Gödöllõ, Hungary. A total of 100 seed cards/round were placed on the soil surface inside a maize field prior to harvest, 10 m from the field's edge, along 25 transects, with four cards/transect. A distance of 10 m was set between the transects and 1 m between the cards. Twenty seeds of each weed species were glued onto sandpaper (25 × 10 cm, P-60), and a wire mesh was used to exclude vertebrate predators. The seed removal was calculated every 24 h, for seven days in 2019 and for five days in 2020, and then the seed predation was measured using the number of removed seeds on each card. The results showed high seed predation levels on all the seed cards, with an overall average of 85.9 ± 13.7%. Besides, there was a decrease in the % of remaining seeds on the cards starting from the first day after exposure due to seed predation in both years. The optimum exposure period for measuring the seed predation was found to be three to four days, though the number of predated seeds on days 3 and 4 significantly differed between years (P < 0.001), with higher predation rates in 2020 than in 2019. However, no differences were detected in the predation rates among the weed species (P = 0.962, 0.079). These findings indicate the potential contribution of seed predation by invertebrates to weed management in Hungarian maize fields.

The effect of water temperature, prey availability and presence of conspecifics on prey consumption of pikeperch (Sander lucioperca) Original Paper

Oleksandr Malinovskyi, Luká¹ Veselý, Carlos Yanes-Roca, Tomá¹ Policar

Czech J. Anim. Sci., 2022, 67(11):465-473 | DOI: 10.17221/162/2022-CJAS

In this study, the effect of water temperature, predator's sex, prey density, and the presence of conspecifics on prey consumption of pikeperch (Sander lucioperca) was experimentally tested. In Experiment 1, predators of both sexes [males: total length (TL) = 473 ± 22 mm and body weight (BW) = 1 070 ± 100 g and females: TL = 464 ± 12 mm and BW = 1 060 ± 100 g] were kept in separate tanks and exposed to different densities of prey (Pseudorasbora parva; 3, 6, 12, 24 and 48 prey fishes per tank) under fixed water temperatures of 4.5 °C, 8.5 °C, and 12.5 °C. After 63 days of this experiment, it was found that pikeperch predation was significantly affected by increasing water temperature. The effect of prey density was significant at all tested temperatures. Pikeperch females tended to have the higher prey consumption than males, although that trend was statistically insignificant. Results suggest that increased feeding demands at temperatures above 4.5 °C can lead to predator starvation in conditions of low prey availability. Due to the higher prey consumption, pikeperch females could be more vulnerable to low prey availability during their culture. In Experiment 2, pikeperch were kept at different densities of 1, 2, 4, and 8 individuals per tank supplied with a prey rate of 50 individuals per predator, ensuring ad libitum feeding rate. The average daily prey consumption was significantly higher in the tanks with multiple predators, accounting for 17.6 ± 3.57 prey fishes/day compared to 11.6 ± 2.33 prey fishes/day in the tank with a single predator. These results indicate that pikeperch predation activity and prey consumption can be significantly affected by the water temperature, prey availability, and the presence of conspecifics. The findings contribute to understanding the predatory function, natural feeding request of pikeperch and its potential importance for broodstock culture and broodstock final maturation for a successful spawning season. Also, this information can be used for better management of pikeperch pond aquaculture or bio-melioration process in open water bodies and ecosystems.

Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forageOriginal Paper

Nevena Banjac, Rade Stanisavljeviæ, Ivica Dimkiæ, Nata¹a Velijeviæ, Marina Sokoviæ, Ana Æiriæ

Plant Soil Environ., 2021, 67(3):130-136 | DOI: 10.17221/581/2020-PSE

Potential of Trichoderma harzianum IS005-12 (TH-IS005-12) to promote seed germination and seedling growth of Italian ryegrass (IRG) forage was evaluated in vitro. Non-desiccated seeds and those pre-harvestly desiccated with total herbicide were treated 25 days (freshly harvested) and 178 days after harvest (mature) with TH-IS005-12 spore suspensions at 0 (T0), 1.8 × 107 (T1) and 1.2 × 109 (T2) spore/mL. TH-IS005-12 promoted the early and final germination and seedling growth in all non-desiccated and desiccated, freshly harvested as well as mature IRG seeds. It was more effective in pre-harvestly desiccated freshly harvested seeds where T2 treatment increased final germination rate for 24%, root number per seedling 1.6-fold and seedling vigour 1.9-fold compared to the untreated control. Moreover, TH-IS005-12 showed an inhibitory activity against seedborne fungi Alternaria alternata and A. ventricosa suppressing their growth in vitro by 82% and 77%, respectively.

Pea transformation: History, current status and challengesReview

Michaela Ludvíková, Miroslav Griga

Czech J. Genet. Plant Breed., 2022, 58(3):127-161 | DOI: 10.17221/24/2022-CJGPB

This review recapitulates the history, important milestones, the current status, and the perspectives of the pea (Pisum sativum L.) transformation as a tool for pea crop breeding. It summarises the developments of the pea transformation from the first methodological experiments to achieving the complete transformation and regeneration of genetically modified (GM) plants, transformation with the first genes of interest (GOI), to recent techniques of targeted genome editing. We show how recent biotechnological methods and genetic engineering may contribute to pea breeding in order to speed up the breeding process and for the creation of new pea cultivars. The focus is laid on genetic engineering which represents an excellent technology to enhance the pea gene pool with genes of interest which are not naturally present in the pea genome. Different methods of pea transformation are mentioned, as well as various GOI that have been used for pea transformation to date, all aimed at improving transgenic pea traits. Tolerance to herbicides or resistance to viruses, fungal pathogens, and insect pests belong, among others, to the pea traits that have already been modulated by methods of genetic engineering. The production of phytopharmaceuticals is also an important chapter in the use of genetically modified peas. We compare different methods of introducing transgenes to peas and also the usage of different selective and reporter genes. The transformation of other major legumes (soybeans, beans) is marginally mentioned. The effect of genetically modified (GM) peas on animal health (feeding tests, allergenicity) is summarised, the potential risks and benefits of pea modification are evaluated and also the prime expectations of GM peas and the real current state of this technology are compared. Unfortunately, this technology or, more precisely, the products created by this technology are under strict (albeit not scientifically-based) legislative control in the European Union.

Romanian wild cherry genotypes (Prunus avium var. sylvestris Ser.) suitable for processingOriginal Paper

Margareta Corneanu, Elena Iurea, Sorina Sîrbu

Hort. Sci. (Prague), 2022, 49(2):95-101 | DOI: 10.17221/73/2021-HORTSCI

This paper presents the important features of indigenous wild cherry genotypes suitable for processing. The research took place between 2017-2020, having ten Romanian wild cherry genotypes as study material. The harvesting maturity was recorded in the first decade of June for the early cultivar (G10) and decades two and three of June for the other studied genotypes, all of them with a middle season maturation. The number of days between the end of the flowering and the maturation was between 54 days (G10) and 66 days (G9). The average weight of the fruit and the equatorial diameter varied between 2.1 g and 13.80 mm for G4, respectively, and 5.5 g and 21.35 mm for G10. The proportion (%) between the pulp and waste (stone and peduncle) was measured as 81.95% pulp (G4) and 93.64% pulp (G10). The values of the soluble dry substance content were between 13.1-24.0 °Brix, the titratable acidity was between 0.48-1.16 mg malic acid/100 mL juice and the total content of the polyphenols was between 229.00-720.00 mg GAE/100 mL fresh juice. In terms of the bitter taste intensity, G10 has a weak intensity, G3, G7, G8 and G9 have an average intensity and G1, G3, G4, G5 and G6 have a high intensity.

Adaptation analysis of insect-resistant transgenic line after introducing mcry1F gene in maizeOriginal Paper

Daming Wang, Junqi Yin, Fengci Wu, Baifeng Wang, Zhilei Jiang, Jingang Liang, Xinyuan Song

Plant Soil Environ., 2023, 69(1):18-24 | DOI: 10.17221/286/2022-PSE

The ability to adapt, survive, and compete with weeds of transgenic plants is the necessary evaluation content to release transgenic lines in target regions. We compared weediness and agronomic traits of transgenic maize lines G1F-8 and G1F-19 carrying the mcry1F gene with their near-isogenic maize inbred line Zheng 58 in the wasteland and cultivated field under natural conditions for two consecutive years. The results showed that there was no significant difference identified in the species, quantity, and relative coverage ratio (RCR) of weeds between fields with G1F-8, G1F-19, and Zheng 58, regardless of the sowing pattern in the wasteland. Compared with the vigour of weeds, none of G1F-8, G1F-19, and Zheng 58 showed survival advantages, and all showed weak growth potential with no final grain yield. Meanwhile, no volunteer seedlings were found upon investigation in the following year. The simulated seed overwintering experiment in the wasteland further showed that the three kinds of maize could not germinate in the second year. In cultivated land, G1F-8 and G1F-19 had the same growth stages, plant height, and RCR as Zheng 58 throughout two years. In conclusion, the transgenic lines G1F-8 and G1F-19 exhibited no adaptability risk in Gongzhuling, Jilin, China.

Gas exchange and chlorophyll fluorescence of four sorghum genotypes under drought stress and rehydratationOriginal Paper

Franti¹ek Hnilièka, Helena Hnilièková, Tomá¹ Rýgl

Plant Soil Environ., 2024, 70(9):543-551 | DOI: 10.17221/292/2024-PSE

Water deficit (drought) is an important environmental factor affecting physiological processes in plants. The present work focuses on the study of changes in physiological responses of juvenile plants (plants in the vegetative phase of growth BBCH 14–16) of selected sorghum genotypes Dokok, 30485, Barnard Red and Ruzrok to water deficit and after rehydration. Water deficit affected the observed physiological parameters – gas exchange and chlorophyll fluorescence. Genotypic differences were also confirmed, with Dokok appearing to be the more sensitive genotype and Ruzrok and Barnard Red appearing to be tolerant. Following rehydration, these parameters increased but did not reach the levels of the control plants. A significant decrease in photosynthetic rate (Pn), transpiration (E) and fluorescence compared to the control was found in the water-deficient variant twice for 10 days and 6 days between rehydration periods. Only in the variant where water deficit (14 days) was followed by irrigation (10 days) transpiration increased in genotype 30485. Chlorophyll fluorescence (Fv/Fm) also decreased significantly in this cultivar. The results suggest that a rehydration period of 14 days is insufficient to restore the photosynthetic functions of stressed sorghum plants.

Does the payment method matter in online shopping behaviour? Study on the Romanian market of vegetables during the pandemic crisisOriginal Paper

Ioan-Sebastian Brumã, Cristina Cautisanu, Lucian Tanasã, Simona-Roxana Ulman, Meda Gâlea, Alexandra Raluca Jelea

Agric. Econ. - Czech, 2024, 70(1):34-47 | DOI: 10.17221/314/2023-AGRICECON


The present study, centred on customer online shopping behaviours, aims at analysing the frequency of vegetable acquisition and its main determining factors, while considering different methods of payment, i.e. cash, debit card, and bank transfer on the Romanian market. Using data obtained from a questionnaire applied nationwide, a descriptive analysis and logistic regression were performed. An immature market in terms of digitalisation, characterised by the customers’ mistrust in online bank transfers, but also in products sold online was observed. In addition, the income, education, gender, and size of the household appeared to be significant in relation to the frequency of online orders for vegetables. Moreover, our results indicated that a higher customer readiness for a high price, large quantity, and a monthly delivery subscription comes bundled with a higher frequency of acquisition in the case of bank transfers. Our findings are especially focusing on the vegetable local producers’ promotion process, with its main desideratum of properly responding through adaptation to the continuously changing customers’ requirements, particularly when special contexts like the pandemic appear. Accordingly, our paper intended to offer several concrete responses in this regard, indicating some possible paths for better managing the communication process on the vegetable market.

Chemical composition and antimicrobial activity of essential oils from six lavender (Lavandula angustifolia Mill.) cultivarsOriginal Paper

Sıla Barut Gök, Yasemin Erdoğdu

Plant Soil Environ., 2024, 70(2):111-123 | DOI: 10.17221/438/2023-PSE

The present study aimed to determine the chemical composition and in vitro antimicrobial potential for the first time of essential oils (EOs) from six cultivars (Druzhba, Raya, Hebar, Hemus, Yubileina, Sevtapolis) of lavender (Lavandula angustifolia Mill.) cultivated in Türkiye (Tekirdağ) against a panel of pathogenic and non-pathogenic microorganisms. The chemical composition of EOs, analysed using gas chromatography/mass spectrometry (GC/MS), revealed 34 different components in the six cultivars. The results indicate that the main major constituents of all EOs were monoterpenoid linalool (47.60–64.13%) and linalyl acetate (12.92–26.08%). Based on principal component analysis (PCA) cvs. Druzhba, Raya and Hebar were situated in the centre right quadrant of the plot and were characterised by linalool and linalyl acetate in subgroup one. The results of antimicrobial assays revealed that lavender EOs were active against all strains of bacteria tested. For bacteria, the strongest activity of cv. Hemus was observed against Enterococcus faecalis (IZ = 19 ± 0.10 mm, MIC = MMC = 6.25 (µg/mL), while the weakest potency was seen against the gram-negative Salmonella enterica (IZ = 21 ± 1.00 mm, MIC = MMC = 31.25 (µg/mL). Based on PCA, the first subgroup of cvs. Hebar and Raya was characterised by Lactobacillus rhamnosus, E. faecalis and Lactobacillus pentosus and was situated in the bottom right quadrant of the plot.

Water content in soil afforested with a mixture of broadleaves or Scots pineOriginal Paper

Jan Vopravil, Pavel Formánek, Tomá¹ Khel, Karel Jacko

J. For. Sci., 2024, 70(2):91-101 | DOI: 10.17221/114/2023-JFS

Afforestation of agriculturally used land has many benefits which are described in different publications. As stated in these publications, afforestation of agricultural land can influence soil water content because of rainfall interception and concentration, transpiration, and properties of forest floor layer and soil; also, hydraulic lift may occur. In this study, we attempted to find changes in volumetric soil water content values (at depths of 20 cm, 40 cm, and 60 cm) on agriculturally used land afforested with a mixture of forest tree species (Quercus robur L., Quercus rubra L., and Acer platanoides L.) or Pinus sylvestris L. in the year 2011 (and on agriculturally used land = control). This study was performed in the period from the end of March 2021 to the beginning of April 2022. In this study, we proved our hypothesis on the reduction of volumetric soil water content after afforestation; it is probably because of higher rainfall interception and evapotranspiration as described in different studies. Concerning the afforestation, the average volumetric water content was higher on the plot afforested with broadleaves compared with Scots pine. It was especially in the period after defoliation when the proportion of throughfall (and stemflow) increased on the plot with broadleaves. Our results indicate a higher water-storage capacity of forests compared with agriculturally used land. During the leafless period, this capacity was found to be higher after afforestation with Scots pine.

Transitional, fused and/or supernumerary vertebrae in the lumbosacrocaudal region of the spine – A reality in many domestic mammalsOriginal Paper

C Toader Covasa

Vet Med - Czech, 2024, 69(5):156-168 | DOI: 10.17221/102/2023-VETMED

The aim of this study was to identify the presence of transitional lumbosacral and sacrocaudal vertebrae in domestic mammals, to realise a comparative analysis of the localisation and conformation of this abnormal condition. The research included the following species: cattle – 29 specimens, sheep – 32 specimens, horse – 31 specimens, pig – 26 specimens, rabbit – 33 specimens, dog – 89 specimens and cat – 57 specimens. The spine of the animals was analysed post-mortem or radiologically. The investigations revealed the presence of transitional vertebrae as follows: in cattle – 3 cases (8.7%), all being about the lumbarisation of the first sacral vertebra (S1); in sheep – 3 cases (9.37%), two lumbarisation cases of S1 and one caudalisation of S4 (the last sacral vertebra); in horses – 4 cases (12.9%), all about the sacralisation of Cd1 (first caudal vertebra); in pigs – 3 cases (11.53%), two lumbarisation cases of S1 and one sacralisation of Cd1; in rabbits – 3 cases (9.09%), a lumbar supernumerary vertebra (L8) and two cases of caudalisation of S4; in dogs – 4 cases (4.49%), a lumbar supernumerary vertebra (L8) and 3 cases of sacralisation of the last lumbar vertebra (L7 or L8); in cats – 3 cases (5.26%), two sacralisation cases of the last lumbar vertebra and one case of caudalisation of the last sacral vertebra (S3). A strong lumbarisation process was observed in ruminants (especially in cattle), then in pigs, the sacralisation being prevalent in carnivores. The sacrocaudal transitional vertebra was predominant in horses. No evident influence of the sex and age of the animals was observed.

Estimation of genetic diversity of sweet sorghum (Sorghum bicolor (L.) Moench) genotypes as a bioethanol source using SSRs markersOriginal Paper

Herniwati Herniwati, Marcia Bunga Pabendon, Karuniawan Puji Wicaksono, Budi Waluyo, Eko Widaryanto

Czech J. Genet. Plant Breed., 2024, 60(2):86-96 | DOI: 10.17221/79/2023-CJGPB

Sweet sorghum is a cereal crop that can potentially serve as a source for bioethanol production. This study aims to analyse the genetic diversity of promising genotypes of sweet sorghum at the molecular level. The genetic material consisted of 12 sweet sorghum genotypes. The genetic diversity estimated using 59 SSRs markers showed a polymorphism value of 0.48 and the coefficient of genetic diversity was classified as moderate. The unweighted pair group method arithmetic average (UPGMA) analysis assigned the tested genotypes into three major clusters with a similarity coefficient level of 0.596. This indicates that the genetic similarity of the tested genotypes is moderate to high. Eight unique loci were identified with the SSRs markers in six genotypes, which are considered to control high sugar traits.

Tree resources decline in Saudi Arabia: Climate change or pest attack causes?Original Paper

Naimah Alanazi

J. For. Sci., 2024, 70(5):223-234 | DOI: 10.17221/9/2024-JFS

In Saudi Arabia, tree mortality has abnormally increased over the past ten years, exceeding regulatory standards without clear interpretation. The present study aimed to investigate three attacked tree populations in different biogeographic regions of Saudi Arabia. For this purpose, climatic data from the last thirty years, forest densities, soil cover, pest search, and insect damage to trees have been reported. The results of this study show a distinct climate change manifested by an increase of 2 °C in temperature accompanied by a decrease in precipitation and the extension of drought periods during the last 30 years. Analysis of satellite images and the computation by quadrat methods revealed a decline in forest density and soil tree cover caused by dieback associated with an increase in the rate of tree pest attacks. Furthermore, tree investigations show attacks of the xylophagous beetles Steraspis speciosa and Paropta paradoxus on Acacia population in the north and centre of the country. In addition, many strains of termites are attacking the Dobera glabra population in the south of the Kingdom. This study shows that tree dieback, as a response to long periods of drought, when associated with pest attacks, provokes a dramatic decline in forest tree heritage. Climate change is the principal precursor to forest decline and pest invasion in Saudi Arabia.

Molecular basis of differential sensitivity to MeJA in floret opening between indica and japonica riceOriginal Paper

Zhiqiang Yan*, Ruyue Deng, Huihui Tang, Hongwei Zhang, Susong Zhu*

Czech J. Genet. Plant Breed., 2024, 60(3):136-148 | DOI: 10.17221/43/2024-CJGPB

Methyl jasmonate (MeJA) accelerated floret opening in rice, crucial for addressing inconsistent floret opening in hybrid seed production of indica × japonica hybrids. However, indica and japonica rice exhibited notable differences in their sensitivity to MeJA’s impact on floret opening, with indica responding faster. To clarify the molecular basis of MeJA-induced floret opening sensitivity differences between indica and japonica rice, after 1 h of MeJA spraying, transcriptomes and endogenous floret opening regulators were compared between indica and japonica rice, with histology differences observed. The results showed that MeJA quickly activated plant hormones in indica and japonica rice, but JAZ genes in JA signalling pathway upregulation were greater in indica rice. Alpha-linolenic acid, starch and sucrose metabolism pathways responded faster in indica rice. MeJA boosted endogenous JA synthesis more in indica rice, which had 5–6 middle-layer cells, more than japonica’s 3–4 layers, resulting in thicker glumes. The metabolic pathways regulating floret opening, including plant hormone signal transduction, alpha-linolenic acid and starch and sucrose metabolism, along with endogenous JA and soluble sugars, could respond to exogenous MeJA. However, the more intense and rapid responses of these regulatory pathways and endogenous substances to MeJA in indica, resulted in the difference in sensitivity to MeJA between indica and japonica rice.

How the milk chemical composition and fatty acid profile are influenced by physiological factorsin Najdi dairy sheepOriginal Paper

Abdulkareem Mohammed Matar, Faisal Ahmed Alshamiry, Ramzi Ahmed Amran, Riyadh Salah Aljumaah, Muneeb Musthafa, Massimo Todaro

Czech J. Anim. Sci., 2024, 69(7):280-291 | DOI: 10.17221/37/2024-CJAS


Sheep milk production is a complex process that is influenced by various factors. This study aims to investigate how the litter size (single vs. twins), birth type (male vs. female), age of the ewes and body weight affect the milk composition and fatty acid profile of milk fat. Milk samples were collected from 119 Najdi ewes that were the subject of this study. Gas chromatography-mass spectrometry (GC-MASS) was used to quantify the fatty acids (FAs). The results showed that the twins birth type (female/female) has a significant influence (P < 0.05) on the concentration of linoleic acid (LA), alpha-linolenic acid (ALA), behenic acid (C22:0) and polyunsaturated fatty acid (PUFA). On the other hand, saturated fatty acids (SFA), docosahexaenoic acid (C22:4; DHA), and odd-chain fatty acids (OCFA), such as C15:0-antiso and C19:1-cis 10, increased significantly (P < 0.05) with the increasing age and body weight of the ewes, while the ALA and unsaturated fatty acids (UFA) significantly decreased (P < 0.05). The principal component analysis (PCA) revealed a positive association between the age and the OCFA, ALA and small-chain fatty acids (C6:0 and C8:0). In addition, the type of birth showed a positive association with the fat, lactose and palmitoleic acid C16:1 cis9. Conversely, there is a negative association between the UFA, monounsaturated fatty acids (MUFA) and PUFA. In addition, the body weight (BW) and litter size were negatively associated with the protein, SFA and medium-chain fatty acids (C10:0, C12:0, C14:0 and C16:0). The physiological factors generally suggested that the milk quality and essential FA, such as ALA, were influenced by the type of the lamb’s birth and the age of the ewes.

A comprehensive review of soil erosion research in Central Asian countries (1993–2022) based on the Scopus databaseReview

Mukhiddin Juliev, Madinabonu Kholmurodova, Bekmurat Abdikairov, Jilili Abuduwaili

Soil & Water Res., 2024, 19(4):244-256 | DOI: 10.17221/82/2024-SWR

Soil is a valuable resource for food production, and it demands a long time to recover itself. Soil erosion is one of the most important issues for countries based on agriculture. This review article analyzed articles published on the topic of general soil erosion in Central Asian countries in 1993–2022. More than 50% of the articles reflect the results of the conducted practical work. The main content of these scientific works is aimed at the prevention of erosion processes, their evaluation and the development of countermeasures. During the considered period, the number of publications on soil erosion has increased in the last years compared to the first years, which indicates that the attention to the topic has increased in recent years. The purpose of this article is to get accurate information about the state of soil erosion in the countries of Central Asia and to get acquainted with the practical works carried out against erosion in these countries. For this aim, the contents of the articles were reviewed and the results of the scientific works conducted on the topic of erosion in each country were presented. According to the content of the articles, the use of modern techniques and technologies in the evaluation and prevention of soil erosion gives effective results.

Effects of abscisic acid and sodium nitroprusside priming on yield and quality of peanut (Arachis hypogaea L.) under drought stressOriginal Paper

Thanh Thang Tran, Thanh Huong Tran, Trang Viet Bui

Hort. Sci. (Prague), 2024, 51(3):219-227 | DOI: 10.17221/97/2023-HORTSCI

This study tested the impact of priming peanut seeds from the VD01-2 cultivar with either 6 mg/L of abscisic acid or 20 mg/L of sodium nitroprusside to prevent damage from drought stress. Throughout the development of peanuts under drought-stress conditions, various morphological, physiological, and biochemical changes were analyzed. According to the study, it was determined that priming with SNP had a more significant effect than priming with ABA. The use of sodium nitroprusside priming resulted in an improvement in both the yield and quality of peanuts when grown under drought conditions. Furthermore, the findings indicate that the use of sodium nitroprusside priming showed an increase in chlorophyll content, gas exchange capacity, and antioxidant activities while reducing lipid peroxidation.

Potential for monetisation and utilisation of wood raw material from pioneer tree species stands in the Czech RepublicOriginal Paper

Roman Dudík, Petra Palátová, Ale¹ Kùs, David Homolka, Andreas Nikodemus

J. For. Sci., 2025, 71(2):99-111 | DOI: 10.17221/101/2024-JFS

Pioneer tree species in the Czech Republic include birch, alder and aspen. From an economic point of view, birch seems to be the most promising. Greater use of timber from pioneer tree species in higher value-added products is currently limited by the low stocks of birch, alder and aspen in forest stands. A price survey was carried out in the Western Highlands region in the central part of the Czech Republic to obtain basic information on the prices of assortments of pioneer tree species. The prices of five assortments of raw timber were surveyed for the period 2019–2023. Very few of the prices of pioneer tree species were found for assortments of II and IIIC; these assortments are not actually produced and, therefore, not traded by the respondents. For the roundwood assortments (quality II, IIIAB), it cannot be clearly stated that any of the preparatory timber species (birch, alder, aspen) always achieved a higher price during the observed period. Only in 2023 was it possible to see higher prices for quality IIIAB assortments of alder. Birch of quality V had the highest price of the observed prices for this timber assortment for the pioneer tree species during the period under review. When using a system approach, considering the information on the prices of the timber assortments is also important for deciding on the optimal species composition of the regenerating forest stands. In the case of the Czech Republic's commercial tree species, this information is available at the national level. However, the situation is quite the opposite for the timber assortments of pioneer tree species, where information on their prices is completely lacking. This confirms the fact that the market for assortments of timber from pioneer tree species in the Czech Republic is basically non-existent, the main reason being the low volume of these assortments placed on the market.

Green guardians: Bacterial endophytes in protecting vegetable crops against pathogensReview

Sagarika Medari, Krishnan Kalpana, Muthusamy Ramakrishnan, Aravindaram Kandan, Subbiah Ramasamy, Karuppiah Eraivan Arutkani Aiyanathan, Sankarasubramanian Harish, Andithevar Beaulah, Rangaswamy Anandham, Narayanan Manikandaboopathi, Marimuthu Ayyandurai

Plant Protect. Sci., 2025, 61(1):21-43 | DOI: 10.17221/38/2024-PPS

Vegetables are considered as the major source for opportunistic and emerging pathogens due to their diverse microbiome. Utilising bacterial endophytes and other bacterial agents to control a variety of economically important plant diseases is vital for achieving sustainable agriculture. Within internal plant tissues, bacterial endophytes form colonies without apparent injury. These bacteria provide several advantages for plant systems, including the direct stimulation of plant development through the creation of metabolites or phytohormones. Importantly, bacterial endophytes play a dual role by safeguarding their plant host through the biocontrol of pathogens and induction of the plant's innate immune system. This review offers a methodical and inclusive examination of the current state of endophytic diversity of bacteria, their methods of plant colonisation and their potential functions as protective agents against plant diseases. The review concludes by proposing diverse effective strategies for applying endophytic bacteria as a biological agent aiming to safeguard vegetable crop plants and enhancing the resilience of agricultural products.

Long-term effects of sugarcane monoculture on soil pedomorphology and physicochemical properties in tropical agroecosystemsOriginal Paper

Anna Kusumawati, Amir Noviyanto

Plant Soil Environ., 2025, 71(3):213-231 | DOI: 10.17221/648/2024-PSE

This study investigates the impact of prolonged sugarcane cultivation on the pedo-morphological characteristics and physicochemical properties of three soil types: Entisols, Inceptisols, and Vertisols, as a basis for determining the improvement step ensuring the sustainability of sugarcane production in Indonesia. Soil samples were collected from fields of sugarcane cultivated for 10, 20, and 30 years to analyse pedo-morphological and physicochemical properties. The results indicate that while Entisols and Inceptisols exhibited significant changes in soil properties with increasing cultivation duration, the pedo-morphology of Vertisols remained relatively stable. All soil types developed Ap horizons due to sugarcane cultivation, with anthropogenic practices leading to more dynamic changes in surface horizons. Extended cultivation reduced soil organic matter, N-total, and available nitrogen, while phosphorus and exchangeable cation availability were influenced by mineral composition. Notably, cation exchange capacity (CEC) decreased in Entisols and Inceptisols but increased in Vertisols. For productivity, Vertisols demonstrated the most stable and highest sugarcane productivity with long-term monoculture cultivation. There is a need for tailored sustainable soil management across different soil types and practices to mitigate soil degradation and maintain nutrient availability to ensure the sustainability of sugarcane production in Indonesia.

Effect of various lengths of cold stratification period on the germination of wild service tree (Sorbus torminalis) seed samples harvested in the Czech RepublicOriginal Paper

Miroslav ©ulitka, Ivan Kune¹, Martin Balá¹, Kateøina Pe¹ková, Pøemysl ©edivka, Tomá¹ Holeèek, Vilém Podrázský

J. For. Sci., 2025, 71(4):171-181 | DOI: 10.17221/61/2024-JFS

The paper deals with the assessment of the influence of the length of cold stratification period on the germination parameters of seeds of wild service tree [Sorbus torminalis (L.) Crantz]. The germination tests (4 × 100 seeds) were conducted in two runs in 2020 and 2021; they were performed for three seed lots and three cold stratification periods (two, three and four months) before the testing of germination in each run. The values of germination energy and germination capacity were assessed, and the germination rate was computed. The results showed that the two-month cold stratification period has not been sufficient to overcome the dormancy of tested seeds. Regardless of the year in which the tests were conducted, seeds stratified for two months showed almost zero germination. For seeds treated with three- and four-month cold stratification, the germination percentage varied from ca. 5% to 32% and from ca. 13% to 46%, respectively. Our results basically correspond with findings reported in the reviewed literature. In 2020, the seeds subjected to four-month stratification performed significantly better than the seeds subjected to three-month stratification. However, in 2021, the seeds stratified for three months reached slightly better results. Four-month stratification also led to an increased percentage of prematurely germinated seeds during stratification. To conclude, the two-month period of cold stratification proved to be insufficient and at least three months of cold stratification are needed to obtain the adequate germination of seeds in subsequent laboratory tests.

Mesobiliverdin IXα-enriched microalgae feed improves gut health in weaned piglets under LPS challengeOriginal Paper

Tz-Shian Liao, Pei-En Chang, Yi-Ting Chen, Ching-Yi Chen, Cheng-Wei Tom Chang, Jon YutakaTakemoto, Yuan-Yu Lin

Czech J. Anim. Sci., 2025, 70(4):139-146 | DOI: 10.17221/159/2024-CJAS


This study aimed to evaluate the effect of mesobiliverdin IXα-enriched microalgae (MBV-SP) on growth, diarrhoea prevalence, intestinal morphology, antioxidant capacity, and inflammatory cytokines in lipopolysaccharide (LPS)-induced enteritis in weaned piglets. Seventy-two 28-day-old piglets were randomly divided into four groups: control (NC), LPS, tylosin, and MBV-SP, with saline/LPS (100 µg/kg) administered intraperitoneally on day 14. Results showed reduced mortality and diarrhoea rates in the tylosin + LPS and MBV-SP + LPS groups compared to the LPS group. Histological analysis revealed improved villus morphology and decreased crypt depth in the MBV-SP group. Catalase activity was notably higher in the MBV-SP group. Both tylosin and MBV-SP supplementation reduced pro-inflammatory cytokines, with MBV-SP exhibiting a more pronounced effect. In conclusion, MBV-SP mitigated diarrhoea, improved antioxidant capacity, and modulated inflammatory cytokines in weaned piglets with LPS-induced enteritis, highlighting its potential as a dietary supplement for the gut health in piglets.

Application of cold atmospheric microwave plasma on four domestic pigeons (Columba livia domestica) with open woundsCase Report

K Zelaya, JH Han, J Kim, SY Ahn, YD Suh, DN Lee, SC Yeon

Vet Med - Czech, 2025, 70(6):218-225 | DOI: 10.17221/13/2025-VETMED

This paper reports the feasibility of cold atmospheric microwave plasma (CAMP) therapy for birds with open wounds. Four domestic pigeons (Columba livia domestica) with open wounds of varying severity were treated with CAMP as an adjunct to conventional therapy. Wound healing was assessed using a modified scoring system during each treatment session, and the extent of healing was calculated as a percentage. The results revealed variability in wound improvement across cases, influenced by the initial severity of the wounds. In some cases, the outcomes were limited due to underlying conditions that overrode the recovery process, suggesting that the extent of healing may depend more on the initial wound condition than the treatment itself. Nevertheless, other cases showed positive results in healing and recovery under CAMP therapy, highlighting its potential benefits. No adverse reactions or complications were encountered, supporting its safety for avian wound management. These findings suggest that although CAMP can potentially promote wound healing, further studies will be needed to establish standardised treatment protocols.

Isolation and identification of herbicidal active substances of Botrytis strain HZ-011Original Paper

Haixia Zhu, Liang Cheng, Yongqiang Ma

Plant Protect. Sci., 2025, 61(4):387-396 | DOI: 10.17221/183/2024-PPS


The herbicidally active Botrytis strain HZ-011 was isolated from naturally infected leaves of Rumex patientia L., and previous studies found that strain HZ-011 exhibits highly effective herbicidal effects against the broadleaf weeds Amaranthus retroflexus L., Elsholtzia densa Benth, Malva crispa, and Chenopodium album L. In this study, the active components of strain HZ-011 were isolated, purified, and structurally characterised using silica gel column chromatography, thin-layer chromatography, and high-performance liquid chromatography. The extraction test was carried out using four organic solvents with different polarities, and ethyl acetate was ultimately used as the extractant for the bulk preparation of the crude product of the active substances. Thin-layer chromatography yielded 10 different fractions and 26 components, and components B3 and E1 showed pathogenic effects on C. album. High-performance liquid chromatography (HPLC) of compound components B3 and E1 yielded three fractions with larger signal values and peak areas, and raw measurements of them revealed that component 3 was active. By analysing and comparing the spectral data of component 3 from 1H-NMR, 13C-MNR, ESIMS, and EIMS, component 3 was identified as dibutyl phthalate. The activity of the individual compounds was verified. The results of this study lay a theoretical foundation for the discovery and exploration of lead compounds. They will also provide a theoretical basis for developing this strain into a microbially-derived herbicide.

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