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This study was conducted to investigate the effects and mechanisms of glutamine (Gln) on the repair of acid-induced injury in dairy cow ruminal epithelial cells (RECs) in vitro. Dairy cow RECs were cultured in a medium with pH of 5.5 for 3 h and subsequently treated with various concentrations of Gln (4, 8, 12, 32 mmol/l) for 12 h. The messenger ribonucleic acid (mRNA) expression levels of occludin (OCLN), claudin 1 (CLDN1), toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and genes for inflammatory factors were measured using reverse transcription-quantitative real-time PCR (RT-qPCR). The results showed that cellular activity and OCLN expression were significantly highest at 8 mmol/l Gln (P < 0.05). CLDN1 expression was significantly higher at 4 mmol/l Gln compared to the other groups (P < 0.05). The relative expression levels of tumour necrosis factor (TNF), interleukin 1B (IL1B), C-X-C motif chemokine ligand 8 (CXCL8), TLR2 and TLR4 in the acid treatment group were significantly higher than those in the control group (P < 0.05), but they were lower in the Gln-treated groups than in the acid treatment group (P < 0.05). These findings demonstrate that Gln promotes the proliferation of RECs, enhances the expression of epithelial cell junction proteins, and inhibits the expression of inflammatory factors and surface receptors. In conclusion, Gln shows a potential for repairing acid-induced injury in RECs.

Contour tillage is an agricultural practice that significantly contributes to enhancing water retention, reducing the risk of flooding, and mitigating soil erosion. Information about the tillage direction is used for modelling water and tillage erosion. In our study, we used Light Detection and Ranging (LiDAR) visualizations, originally developed for archaeological research, to visualise tillage traces and map tillage direction in the Nitra district (SW Slovakia). The tillage traces were visible in all agricultural parcels, on various agricultural fields, under various agricultural crops. The LiDAR visualisations also revealed pre-collectivization field patterns and even prehistorical field patterns in certain areas. Among the 5 961 investigated points, we recorded the application of contour tillage in 30.63% of the cases. The preference for contour tillage varied among farmers, with the highest reported percentage reaching 49.74%. Our analysis did not reveal a significant correlation between the preference for contour tillage and the slope steepness.

The study aims to identify the difference between the current and future strategic management of forest ecosystem services (FES) portfolios in public enterprises of Slovakia. The case studies focus on forest enterprises in Košice, Banská Štiavnica, Kremnica, and Bratislava, representing the best practice examples of providing cultural services in the country. A marketing decision-making model, Boston Consulting Group (BCG) matrix, was used to analyse each company's current FES portfolio. Content analysis assessed the data collected from interviews with managers of the public forest enterprises. Duncker's forest management approaches classification was applied to evaluate the future strategic management of the FES portfolio, and a horizon of 10 years was set. According to the results, the urban forest enterprise in Bratislava is an example of receiving a subsidy from the capital for building and reconstructing recreational facilities. Therefore, this FES portfolio is and will be the most balanced. The portfolio of the forest enterprises in Košice, Kremnica, and Banská Štiavnica will remain unchanged for the next decade, and they plan to use a combination management approach that caters to economic, ecological, and social needs and objectives. Urban forests in Bratislava will focus even more on close-to-nature forestry.

Global climate change (GCC) is putting increasing pressure on forest ecosystems, leading to more frequent disturbances such as pest outbreaks and other climate-related stressors, all of which threaten forest stability. This study examines how different technical water retention measures (infiltration pits) can enhance the resilience of European beech (Fagus sylvatica L.) to these climatic challenges, focusing on their impact on radial growth, sap flow, and acclimatisation to moisture conditions at two sites in Czechia (430–440 m a.s.l.). Three treatments were compared: a water infiltration pit under a culvert mouth, an infiltration pit without a culvert and a control plot without a technical solution. Results showed that maximum daily transpiration rates of beech ranged between 90–120 L per day. Air temperature had a stronger influence on beech radial growth than precipitation, particularly at the waterlogged sites. The lowest radial growth occurred in the treatment involving a water infiltration pit under a culvert mouth, while treatments with an infiltration pit without a culvert demonstrated notable seasonal stem shrinkage and swelling (tree water deficit – TWD), especially in early spring. On the other hand, no differences were found between the three treatments including the control variant in the maximum growth or the context of minimum TWD. In conclusion, these technical measures had limited or short-term effects on the growth and physiological processes of European beech. Despite the high costs of implementation, sap flow and dendrochronological measurements do not support the construction of infiltration pits as a means of improving water retention in forest ecosystems.

The aim of the research was to demonstrate the possibility of using hydrogen peroxide with silver (H₂O₂-Ag+) and selected fungicides for treating narcissus bulbs and their impact on plant growth and development. In the experiments were used compounds such as hydrogen peroxide with silver (H₂O₂-Ag+) and captan (Biszop 80 WG), pyraclostrobin + boscalid (Signum 33 WG) and methyl thiophanate + tetraconazole (Yamato 303 SE) to soak narcissus bulbs before planting for the period of 20 minutes. The research showed that stimulation of growth and development by some of the tested concentrations of H₂O₂-Ag+ used to treat bulbs was shown with regard to plant height, leaf length, fresh flower weight, dry flower weight, the number of days from taking plants out of cold storage to flowering, the weight of fresh above-ground parts without flowers, the weight of dry above-ground parts without flowers, the diameter of the flowers, the height of the flowers, petals length, the width of the petals and the length of the corolla. In turn, the tested fungicides stimulated the length of leaves, the fresh weight of plants without flowers, and Signum 33 WG and Biszop 80 WG also the dry weight of flowers. No phytotoxicity of hydrogen peroxide with silver and tested fungicides was found for narcissus.

Drawing upon data on co-signed patents in China's agricultural sector between 2015 and 2022, this paper explores the structural characteristics and determinants of the patent collaboration network in agricultural technology involving universities (U), enterprises (E) and research institutes (R). The results of social network analysis (SNA) revealed that the patent collaboration network is expanding in scale, but innovators are sparsely connected to others. Although the subnetwork linked by enterprises is the largest, universities and research institutes are more likely to play roles as hubs and bridges in the network. Furthermore, quadratic assignment procedure (QAP) regression revealed that prior collaboration experience and geographical proximity are key factors that promote co-patenting in the agricultural sector. Compared with U–U partnerships, E–E and E–R partnerships are associated with decreased patent collaboration. In the agriculture and forestry industries, the U–U and U–R partnerships are most likely involved in co-patenting, followed by the R–R and U–E partnerships. In the animal husbandry and fishery industries, no significant difference was found between the partnerships of U–U, R–R, U–E and U–R in their collaborative propensity.

Nanotechnology plays a vital role in enhancing plant tolerance to salt stress; however, comparative studies on zinc oxide bulk particles (ZnO bulk) and zinc oxide nanoparticles (ZnO NPs) in this context remain unexplored. Since zinc (Zn) is an essential micronutrient involved in enzyme activation, photosynthesis, and antioxidant responses, it is important to understand how ZnO bulk and ZnO NPs influence chickpea growth under salt stress. This study investigated the morphological and physiological responses of chickpea seedlings treated with ZnO bulk (50 mg/L) and ZnO NPs (50 mg/L) under varying salt concentrations (20, 40, 80, and 120 mmol/L). Salt stress significantly inhibited chickpea growth, reducing the relative growth rate, net assimilation rate, total chlorophyll content, and potassium (K) and zinc ion levels while increasing sodium (Na), chlorine (Cl), malondialdehyde (MDA), and proline content. However, the application of ZnO bulk and ZnO NPs improved these parameters, mitigating the negative effects of salt stress. Furthermore, exogenous ZnO bulk and ZnO NPs to salt-stressed (20, 40, 80, and 120 mmol/L) chickpea resulted in decreased malondialdehyde content by 30, 32, 47, 34%, and 58, 31, 48, 47%, proline content by 4, 6, 1.6, 4% and 22, 21, 22, 28%, respectively, in comparison to the control. Notably, ZnO bulk and ZnO NPs enhanced antioxidant enzyme activities, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione peroxidase, and glutathione reductase. These findings suggest that foliar application of ZnO bulk and ZnO NPs helps alleviate salt stress in chickpeas, promoting better growth and physiological performance under saline conditions.

The darkness of the kernel and the lack of simultaneous ripening of the mesocarp and endocarp are among the most significant challenges in Iranian walnut orchards. The mentioned problems appear more in areas with hot and humid summers. The research focused on the quality of the kernel in terms of colour and marketability, as well as the dry matter of the kernels. Additionally, the ease of mesocarp separation from endocarp was examined. Experimental trials were designed with 27 treatment combinations to address the mentioned issues using varying concentrations of 2-chloroethylphosphonic acid (ethephon) at different harvest dates. In this study, using 1 920 ppm of ethephon on August 22nd resulted in the best quality walnut kernels and mesocarp. Researchers in this project successfully achieved the highest quality of walnut kernels and nuts in a region where over 90% of the harvest consisted of dark-kernel walnuts.

A free electricity tandem-twin-hybrid-solar-biomass dryer comprised of two drying rooms and operated with solar and biomass energy combustion of 10 kg rubber wood per hour separately to dry Robusta coffee cherries with 3, 6, 9, and 12 cm bed thicknesses were studied with the drying completion time (tc), number of defects (ND), and colour parameters, i.e., lightness (L*), hue angle [H(^o)], and chroma (C), used as the performance indicators. The experimental results indicated that the drying room, bed thickness, and drying room-bed thickness interaction significantly affected the tc and ND and bed thickness only significantly affected C for both the solar energy drying and the biomass energy drying. The solar energy drying generated a drying air temperature of 44.6 ± 3.5 °C with a tc of 70.9–90.2 h for the front drying room and 40.1 ± 2.8 °C with a tc of 77.2–116.5 h for the rear drying room, whereas the biomass energy drying produced a drying air temperature of 57.2 ± 3.6 °C with a tc of 34.1–44.9 h for the front drying room and 45.6 ± 6.0 °C with a tc of 56.3–96.6 h for the rear drying room. Both drying processes produced coffee beans with the NDs less than 11 qualified for Grade 1 with similar colour characteristics.

To promote the sustainable consumption of prepared dishes, this study examines how green attribute information (GAI) and live streaming scenes experience (LSSE) influence consumers' green perceptions (GP) – including perceived green value (PGV) and perceived green trust (PGT) – and subsequently affect their purchase intention (PI). A green perception model was developed and tested using a mixed-method approach. Word cloud analysis was conducted on consumer comments collected from e-commerce platforms to extract key descriptors and concerns, which informed the design of a structured questionnaire. A total of 291 valid responses were analysed using partial least squares structural equation modelling (PLS-SEM). Results show that: (i) GAI does not directly impact PI, indicating that GP is a necessary mediator; (ii) GAI significantly influences PGV, but not PGT; (iii) GP fully mediates the GAI–PI relationship, although PGV has a negligible direct effect on PGT; (iv) LSSE significantly enhances both GP and PI; (v) GP partially mediates the LSSE–PI relationship. These findings highlight the dual role of LSSE in facilitating information adoption and emotional engagement, and suggest that enhancing LSSE design and enriching GAI are effective strategies to increase consumers' green perception and purchase intention towards prepared dishes.

The Ili region hosts China’s largest lavender cultivation base, yet soil bacterial diversity in its primary cultivation areas remains understudied. To address this, we compared soil bacterial communities across four major cultivation counties (Chabuchar, Agricultural Research Institute, Yining, and Huocheng). Essential oil profiles, soil properties, and bacterial community characteristics were analysed to elucidate microbial variations and environmental interactions. The results showed that: (1) The essential oil yield (1.14%) and linalool content (41.04%) in the Huocheng County cultivation area were significantly higher than those in other areas, and the essential oil quality was relatively the best; (2) the soil bacterial communities in different main cultivation areas shared certain commonalities. At the phylum level, Proteobacteria, Acidobacteriota, Gemmatimonadota, and Actinobacteriota were the dominant phyla, and their relative abundances varied by region and soil layer, and (3) the redundancy analysis results showed that soil bacterial communities were comprehensively affected by environmental factors such as pH, total nitrogen, total phosphorus, soil organic carbon, longitude, and altitude. The significant positive correlations between the abundance of Vicinamibacteraceae (Acidobacteriota) in Huocheng County soils and both soil total phosphorus and linalool content suggest a putative mechanism whereby this bacterial taxon enhances lavender terpenoid synthesis by facilitating phosphorus cycling. Overall, these results suggest that geographically driven climatic variations dynamically alter the soil bacterial community, thereby influencing lavender growth and the final essential oil quality.

This paper analyses the market entry strategy of the Spanish wineries and their destination markets. For this purpose, channel adjustment was analysed. The novel aspect of this research is the use of exports on a winery level, basing the explanation of their export performance on a combination of internal decisions of the companies, and characteristics of the wine destination markets, in accordance with the purchasing patterns of their consumers. The results revealed the importance of strategic adjustment between winery and market destination, even though the importance of adjustment depends on the level of exports. Thus, channel adjustment is important for the probability of export and export intensity.

This study aims to investigate using different ratios of urea-molasses in silage of food in vitro fermentation, feed value and quality production in feed. After being withered a little, the fodder plant was fragmented into 2–3 cm long pieces. 0, 2 and 4% molasses and 0, 0.5 and 1% urea were added. The treatment groups were UM1 (control), UM2, UM3, UM4, UM5, UM6, UM7, UM8 and UM9 supplemented with 0, 0, 0, 1, 1, 1, 1.5, 1.5, 1.5% urea and 0, 2, 4, 0, 2, 4, 0, 2, 4% molasses respectively, and a total of 54 silage samples, 9 × 6 parallel, were prepared, vacuumed in ready-made silage bags, and stored at 25 ± 2 °C for 60 days. After the silages were opened, pH, nutrient contents, Fleig scores (FS), in vitro digestibility parameters, energy contents, feed value and feed quality were determined, then the data were subjected to analysis of variance. The effects of urea and molasses additives added to the mixed forage plant silage on all parameters were found to be significant (P < 0.05, P < 0.01). The crude protein (CP) increased its application by UM7. Cell wall component (insoluble fibre in acid detergent (ADF) and neutral detergent insoluble fibre NDF) reduced its application by UM9. All these applications increased the FS. The addition of molasses increased the in vitro gas production, while the addition of urea decreased the production. True organic matter digestibility increased with the addition of molasses. As a result, it was concluded that UM9 application in silages would be appropriate for increasing feed value and feed quality, and the UM3 application in silage would be appropriate for improving true organic matter digestibility (TOMD).

The rise in demand for animal products associated with global population growth has driven the world toward precision livestock farming, where convolutional neural networks (CNN) have gained increasing attention due to their potential to enhance animal health, productivity, and welfare. However, the effectiveness and generalizability of CNN applications in cattle production are limited by several challenges and limitations, which require further research and development to address. This systematic literature review aims to provide a comprehensive overview of the applications of CNN in cattle production. It identified some potential applications of CNN in this field and highlighted the challenges and limitations that need to be addressed to improve the effectiveness and efficiency of CNN applications in cattle production. It also provides valuable insights for researchers, practitioners, and policymakers interested in the use of CNN to enhance cattle production practices, animal welfare, and sustainability. Additionally, it also provides the reader with a summary of the literature on the fundamental concepts of convolutional neural networks and their commonly used model architectures in cattle production. This is because agriculture digitalisation is going more multidisciplinary and people from different areas of expertise may find it helpful to learn more from a combined source.

Low water availability is a significant constraint on global crop production. Exploration is needed regarding plant responses to drought in interaction with biochar, encompassing optimised water use and carbon allocation strategies. The size of the biochar particles also plays an important role, especially in influencing the dynamics of water and plant growth. This study explored the potential impact of biochar treatment on radish growth and drought tolerance. Finer biochar particles lead to the most substantial available water content for plants, increasing at around 30%, while medium and larger fractions increase by about 22% and 16%, respectively, compared to control soil. The chlorophyll fluorescence technique showed improved water management of drought stress at larger fractions of biochar. Our research underscores the potential of biochar treatments for environmental stresses and water scarcity in modern agriculture.

The paper deals with a new method of innovative assessment of the supported effects and ecosystem services of forests using the actual amount of their increased costs. Among the most important research findings and significance of the new method is that it contributes to the body of knowledge on valuation and payment for ecosystem services. The focus of the research task is to help address the current difficult economic situation of forest owners resulting from the impacts of climate change. The study aims to determine what work quantifies the amount of compensation for the provision of supported ecosystem services that is sufficient for owners by using hypothesis testing. The evaluation of the supported effects was carried out in a case study in the area of the Chrudim-Podhůra Recreational Forests in the Czech Republic. The results of the evaluation were quantified in two experimental spreadsheets of the results, a comparative analysis of the evaluation results was performed, and hypothesis testing was conducted. The innovative methodological approach to the assessment of supported effects can be easily modified and used to assess the actual amount of increased costs of other ecosystem services of the forest.

The global demand for high-quality, nutritious food is increasing, and mushrooms have gained popularity as a healthy dietary option; mushroom consumption is increasing in Pakistan owing to their rich nutritional profile, phytochemicals presence and antioxidant capacity. This study was carried out to determine comprehensive nutritional analysis, antioxidant parameters, amino acids, and fatty acid composition of commonly grown mushroom varieties oysters (Pleurotus ostreatus) and white buttons (Agaricus bisporus) in Pakistan. The study results indicated mushrooms are perishable, high in protein and fibre content, along with a low caloric value. Also, they are rich in essential minerals like potassium, phosphorus, iron, and water-soluble vitamins. In addition, both mushrooms demonstrated significant antioxidant activity, with oyster mushrooms having a higher potential. Additionally, profiling of fatty acids and amino acids showed mushrooms having low caloric value and powerful nutrition composition that makes them suitable for developing healthy dietary choices for better human health.

The present investigation explored the possible use of Thymus vulgaris essential oil (TVEO) as a beverage antifungal preservative instead of chemical ones. The chemical profile of TVEO exposed carvacrol (60.47%) as the predominant compound. The antifungal properties of TVEO were assessed on various food spoilage yeast and mould species using two tests. TVEO showed a powerful antimicrobial effect against all the fungal strains at the three volumes of essential oil (EO) used (i.e. 10, 20, and 30 µL). The minimum inhibition concentration (MIC) of TVEO was also evaluated and ranged from 0.0625% to 0.015% (v/v). Furthermore, the potency of TVEO as a beverage antimicrobial preservative was tested at four distinct concentrations (0.6, 1.25, 4, and 6 μL·mL^–1) against Saccharomyces cerevisiae alone and combined with medium heating (70 °C for 2 min) in a real food matrix (Orangina^® drink) for eight storage days. TVEO exhibited a significantly higher preservative effect than chemical preservatives (sodium benzoate and potassium sorbate). Lastly, a molecular docking examined the mechanism of action of carvacrol against two crucial enzymes in S. cerevisiae viability [ERG2 (sterol C8-isomerase) and ERG3 (sterol C5-desaturase)] compared to a chemical preservative (potassium sorbate). The two ligands highly interacted with the two target enzymes. However, carvacrol achieved a better score than potassium sorbate against ERG2 and ERG3, with binding energy of –10.19 kcal·mol^–1 and –11.73 kcal·mol^–1, respectively. Our results open up the perspective of using TVEO as a natural food preservative.

Choerospondias axillaries (CA) is an important fast-growing afforestation tree species in southern China, and its fruit has medicinal and edible value. High performance liquid chromatography was used to determine the composition and content of sugar and acid in CA fruits from different provenances, and cluster analysis was conducted on different provenances. The results showed that the total sugar content of CA fruit ranged from 49.31 to 139.41 mg/g, with sucrose accounting for the highest proportion of total sugar, followed by glucose, and fructose was the lowest. The total acid content of CA fruit ranged from 47.97 to 82.81 mg/g, with citric acid accounting for 67.09% of the total acid, followed by ascorbic acid, quinic acid, tartaric acid, and malic acid. Cluster analysis was conducted on 20 CA fruits, which were divided into 4 categories. It was recommended to develop N19 fruit had the highest content of sucrose and glucose, and the highest sweetness value, sugar-acid ratio and sweet-acid ratio. It can be suggested to be developed as a high-sugar fresh food source. N02 fruit with high sugar and high acid content can be used as a raw material for fruit cake processing. This result provides an important reference for the quality evaluation and rational development and utilization of CA.

Knowing the relationship between forest soil properties and their stand conditions is relevant for the sustainable exploitation and management of forest soils. This study examines the influence of stand environmental factors on soil properties within forest environments. We further assessed the spatial variability of these soil properties and their controlling factors. A harmonised soil database on the entire forest areas of the Czech Republic was considered; however, only 851 sampling points with complete data on soil properties was used out of the more than 8 thousand sampling points in the database. The topsoil mineral layer of 0–30 cm was analysed. Principal component analysis was used to determine the relationships between the forest soil properties and their stand controlling factors. The nugget ratios for the semivariograms and cross-variograms were used to evaluate the spatial dependence of soil properties, and their relevant controlling factors. Forest types influence soil reaction and the availability of cations within the topsoils. Phosphorus is influenced by aluminium and cation exchange capacity. There are higher concentrations of total phosphorus and aluminium under broadleaved forest.

The potential use of volatile compounds released from milled seeds of mustard (Brassica juncea cv. Malopolska) obtained from three different companies was tested in in vitro and in vivo experiments for their inhibitory effect on Botrytis cinerea growth on agar media and its infection on vegetable leaves of cucumber, bean and spinach. In the experiments with spinach, the effect of volatiles from mustards on the storage and sensory quality of fumigated leaves was evaluated. The antifungal effect of the volatiles depended on the source and dosage of mustard seeds and biofumigation time. The most efficient inhibition of B. cinerea mycelium growth on agar media and vegetable leaves was mustard S from SHR company. The development of grey mould on spinach leaves was inhibited in the treatment with 4 h biofumigation with the volatiles from mustard S seeds in experiments conducted at 10 °C and also at 18 °C. In the sensory and storage quality analysis, the spinach leaves treated with volatiles from mustard seeds showed acceptable parameters that predisposed the product to consumption. The results show that it is possible to reduce the incidence of vegetable grey mould with the treatment of milled mustard seeds, opening a potential application of biofumigation in the control of B. cinerea in vegetables.

Calcareous soils, typically characterized by low fertility, low organic matter and nitrogen content, and often deficient in phosphorus, zinc, and iron, as well as having low microbial activity, require the development of sustainable soil conditioners to improve fertility. To address these shortcomings and promote sustainable agriculture, biochar, especially with acidic character, may offer a promising solution. This study investigates the effects of modified biochar by H₂SO₄ and ZnSO₄ on soil properties and wheat yield under field conditions. For this purpose, biochar (B), acidified biochar (AB), Zn enriched biochar (BZn), and acidified-Zn enriched biochar (ABZn) were applied to the field at two different doses (0.5 and 1.0%) together with the control treatment (Ck) without biochar application. AB_1.0% was determined as the most effective treatment in decreasing soil pH (0.15 units), while B1.0% was determined as the most effective treatment in increasing organic carbon and cation exchange capacity, 13% and 32%, respectively. The effect of the treatments varied for specific nutrients. The highest antioxidant enzyme activities were found in acidified biochars where the lowest yields were obtained. Compared to the Ck, the highest catalase (CAT) (32%) was determined in ABZn_1.0%, ascorbate peroxidase (APX) (56%) and glutathione peroxidase (GPX) (36%) were determined in ABZn_0.5%, and superoxide dismutase (SOD) (28%) was determined in AB_0.5%. The highest proline (PRO), with the least decrease in yield, was found in the AB_1.0% application, which is 205% more than Ck. B and BZn treatments all increased the grain yield, and the highest increase was 20% in B_1.0% when compared to the Ck.

The availability of a broad variety of cultivars in many ornamental species has increased recently, in particular for attractive annuals, which are valued commercially for their use as cut flowers, potted plants, loose flowers, and in landscape gardening. The breeding of ornamental plants in the current scenario is a challenging endeavour with constantly evolving new obstacles. Modern genomic technologies provide prospects for improved precision breeding and selection for characteristics that are more difficult to determine. Traditionally, ornamental breeding has been focused on increasing resistance to biotic or abiotic stress, novelty, yield, and quality. However, accomplishing these objectives necessitates tedious cross-breeding, and exact breeding methods have been noticed to be not applied constantly. Though the purposes of ornamental crop breeding may vary, the process generally does not differ from the breeding of other crops. Furthermore, vegetatively propagated ornamentals constitute most of the crops. The expanding interest in ornamental crops that are produced by modern crop breeding methods such as genome editing, chromosome manipulation, molecular marker-assisted breeding, mutation breeding, and exploiting somaclonal variations, particularly in relation to altering desirable plant features and producing new ornamental traits of the crops which is the main objective of crop improvement practices. Hence, it has become obligatory to evaluate the current state of any technology created following an in-depth study carried out by several research organisations.

The increasing global demand for energy and the negative environmental impacts of fossil fuel exploitation have driven interest in sustainable energy solutions, such as wood. The Russian Federation, as one of the world's largest pellet producers, has an opportunity to utilise pellets domestically. This study addresses the lack of publicly available life cycle assessment (LCA) studies on pellet production and utilisation in Russia, specifically examining the environmental impact of residential heat production from locally produced wood pellets. Utilising primary data from the Northwest region, the study follows ISO 14040 and 14044 standards and employs the ReCiPe 2016 (H) Midpoint v. 1.1 method to assess environmental impacts. The results indicate that the production of pellets is the dominant contributor to the global warming impact category, marine eutrophication, and fossil resource scarcity, while transportation has the least impact across all categories. Sensitivity analyses confirm the robustness of these findings, revealing that using natural gas for pellet drying increases emissions for global warming and fossil resource scarcity, and increasing transportation distance significantly raises emissions across all categories. The findings provide valuable insights for policymakers and stakeholders aiming to enhance the sustainability of similar bioenergy systems.

This study examined the genetic variability, heritability, and genetic advance in the F₄ generation of sorghum to enhance grain productivity. The study was conducted at Citayam Research Station, Depok Indonesia (March–September 2023), and evaluated 102 progenies of the F₄ population from a Bioguma (a mutant line) × Gando Keta (a local variety) cross using an Augmented block design with four replications. The results of this experiment indicated that the F₄ generation outperformed the Gando Keta grain yield components, but remained inferior to Bioguma. The yield traits were influenced by the non-additive gene action, with genetic factors playing a significant role in grain weight variation. The top 20% of F₄ progenies exhibited improved yield characteristics, including thicker stems, larger panicles, and increased grain weight.

Sufficient available water in the soil is critical for plant growth, especially soon after planting. Water absorbed by organic hydrosorbents has the potential to reduce drought stress after planting and improve seedling survival. This research evaluates the effectiveness of granular organic hydrosorbents (GOH) in increasing soil-water retention in a pot experiment with boxwood (Buxus sempervirens L.) seedlings. The leaf chlorophyll content was also evaluated to determine plant growth. The following fertilisers were used: GOH 1 comprised 50% biogas products and 50% wheat straw, and GOH 2 combined post-harvest grain, legume and oilseed residues. The fertilisers were applied to the bottom of the pot or mixed with the soil. Both types doubled the initial water retention compared to the control. While the control water retention dropped below zero after 18 days without watering, retention in the fertilised treatments ranged from 0.5 to 0.76 L. Re-watering part of the trial after the 18 days then induced increased retention to the initial levels in all treatments and the control. However, the fertilised plant’s water retention was up to half a litre greater than in the control when the 62-day experiment ended. The effect of GOH application was also manifested in the chlorophyll content. In the no watering mode, the chlorophyll content was, on average, 20% and 13% higher in the treatments with GOH 1 and GOH 2 than in the control. In the watering regime, the application of GOH 1 and GOH 2 increased the chlorophyll content by an average of 31% and 26%, respectively. Finally, these trials established that the GOH applications significantly delayed boxwood-seedling drying and increased their water retention and chlorophyll content in unwatered and re-watered plants.

Corn poppy (Papaver rhoeas L.) is one of the most problematic weed species, mainly in rainfed Moroccan and Tunisian cereal crops. The overuse of acetolactate synthase (ALS) inhibiting and/or auxinic herbicides led to the spread of corn poppies resistant to both chemical families in this region. In order to identify and understand the selection drivers of resistance, appropriate characterisation of the resistance profile is necessary. Two experiments were carried out: biological sensitivity tests with ALS inhibiting herbicides (tribenuron-methyl and florasulam) and auxinic herbicides (2,4-d) were carried out with populations sampled in the field where the herbicide failure was observed. Bioassay tests confirmed resistance in all studied populations with an average frequency of 75.13, 30.81, 33.17 and 11.52% with tribenuron, florasulam, 2,4-d and florasulam + 2,4-d, respectively. Corn poppy sampled from both countries exhibited similar frequencies within populations for each tested herbicide. The molecular analysis was conducted with next-generation sequencing (Illumina), allowing massive, precise and rapid sequencing regions of the ALS gene carrying resistance codons. Using this technology, ALS mutant alleles were found in all populations at frequencies ranging from 1.4% to 63.3%, with an average of 16.7%. This study highlights the need to elucidate resistance mechanisms to understand herbicide responses and develop effective strategies for managing resistant corn poppy in rainfed cereals as an essential step to maintain the effectiveness of these molecules as long as possible.

Fusarium wilt disease presents a substantial challenge to tomato production, especially in an open field environment. The peroxidase (POD) activity and total phenolic compounds (TPCs) play a crucial role in measuring the antioxidant capacity of plants. Understanding the variations in the POD and TPC levels during disease-induced stress becomes important for effectively managing Fusarium wilt and enhancing tomato production. This study investigates the impacts of Trichoderma harzianum inoculation through the root drip method on five tomato cultivars. It compares these cultivars to their non-treated counterparts when they are subjected to infection by Fusarium oxysporum f. sp. lycopersici (Fol). The results showed that the level of resistance to Fol is based on the specific tomato cultivar. Notably, ‘MT26’ exhibited the lowest disease severity index (DSI), indicating a strong response, whereas ‘CLN3682F’ showed notable susceptibility. Regarding the POD and TPC activity, its exhibition differed in compatibility with the response of each tomato cultivar to Fusarium wilt disease. The resistant cultivars increased the POD activity after the Trichoderma induction before the Fol inoculation, and this activity was further boosted when exposed to disease conditions. Consequently, enhancing the POD and TPC levels during the initial stages could potentially serve as a systemic defence mechanism of tomatoes against the Fusarium wilt disease.

The current investigation was done to study the impact of adding banana peel powder (BPP) and treatment with microbial transglutaminase (MTGase) on the structure, physicochemical, antioxidant, and sensory qualities of yoghurt prepared from camel milk powder during cold storage. BPP analysis showed that it contained high amounts of total phenolics, which were accompanied by strong antioxidant activity. When employed alone or with BPP, MTGase polymerises yoghurt proteins, forming a structure with a high molecular weight above the stacking gel and lacking pores. The levels of protein, total phenolic compounds (TPC), total solids, ash, and antioxidant activity (DPPH – 2,2-diphenyl-1-picrylhydrazyl) increased after the addition of BPP to yoghurt, even after 21 days of storage. Adding BPP to yoghurt initially decreased thiobarbituric acid reactive substances (TBARS) levels, which then increased with the storage time. Yoghurt made with BPP and treated with MTGase was more stable with enhanced physicochemical, antioxidant, and sensory qualities during storage when compared to yoghurt made with MTGase alone. The results of the study demonstrated that MTGase is capable of cross-linking yoghurt proteins and that BPP can be applied as a stabiliser, antioxidant, and functional component in yoghurt production.

The purpose of this study was to assess the amino acid and fatty acid profiles of swamp buffalo meat (Bubalus bubalis) obtained from a local market. The extractable free amino acids and free fatty acids of raw and cooked loin (Longissimus lumborum: LL) and round (Semimembranosus: SM) buffalo meat were analysed. These experiments were performed in a 2 × 2 factorial in randomised complete block design (RCBD) and there were four treatments combinations, including raw-loin, cooked-loin, raw-round and cooked-round with five replications. The results revealed the amino acid composition for the raw-loin included leucine [4.51 mg·(100 g)^–1 sample) and isoleucine [4.56 mg·(100 g)^–1 sample], whereas methionine, aspartic acid and asparagine were the least common amino acids found in both raw and cooked swamp buffalo meat [0.002, 0.020 and 0.034 mg·(100 g)^–1] of the sample. The amino acid composition of the cooked meat decreased by 50% as compared to raw meat. Swamp buffalo meat contains 10 fatty acids, including saturated fatty acids (SFAs) such as myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0) and docosanoic acid (C22:0); monounsaturated fatty acids (MUFAs) such as palmitoleic acid (C16:1, n-7) and oleic acid (C18:1); and polyunsaturated fatty acids (PUFAs) such as linoleic acid (C18:2, n-6, ω6), gamma-linolenic acid (C18:3, n-6, ω6), eicosatetraenoic acid (C20:5, n-3, ω3) and docosahexaenoic acid (C22:4, n-6, ω6). Stearic acid (C18:0) was the most abundant saturated fatty acids found in the lipid component of buffalo meat. Other medium- and long-chain saturated fatty acids (C14:0, C16:0, C22:0) contributed to around 3–4% of the total fatty acid composition. The most abundant MUFAs and PUFAs were oleic acid (C18:1) and eicosapentaenoic acid (C20:5). The PUFA/SFA ratio, total polyunsaturated fatty acids n-3, total polyunsaturated fatty 64 acids n-6, and n-6/n-3 ratios were not significantly different between raw and cooked buffalo meat.